Novel nucleotide and amino acid sequences, and assays and methods of use thereof for diagnosis of ovarian cancer

ABSTRACT

Novel markers for ovarian cancer that are both sensitive and accurate. These markers are overexpressed and/or differentially expressed in ovarian cancer specifically, as opposed to normal ovarian tissue. The measurement of these markers, alone or in combination, in patient samples provides information that the diagnostician can correlate with a probable diagnosis, in ovarian cancer. The markers of the present invention, alone or in combination, show a high degree of differential detection between ovarian cancer and non-cancerous states.

CROSS-REFERENCE TO RELATED APPLICATION(S)

THIS APPLICATION IS RELATED TO NOVEL NUCLEOTIDE AND AMINO ACID SEQUENCES, AND ASSAYS AND METHODS OF USE THEREOF FOR DIAGNOSIS OF OVARIAN CANCER, AND CLAIMS PRIORITY TO AND INCORPORATES HEREIN BY REFERENCE (IN THEIR ENTIRETY) EACH OF THE CORRESPONDING NON-U.S. PROVISIONAL APPLICATIONS AND THEIR CORRESPONDING U.S. PROVISIONAL APPLICATIONS NOTED BELOW:

APPLICATION No. 60/620,916 FILED Oct. 22, 2004—DIFFERENTIAL EXPRESSION OF MARKERS IN COLON CANCER

APPLICATION No. 60/628,123 FILED Nov. 17, 2004—DIFFERENTIAL EXPRESSION OF MARKERS IN COLON CANCER II

APPLICATION No. 60/621,131 FILED Oct. 25, 2004—DIAGNOSTIC MARKERS FOR COLON CANCER, AND ASSAYS AND METHODS OF USE THEREOF

APPLICATION No. 60/620,917 FILED Oct. 22, 2004—DIFFERENTIAL EXPRESSION OF MARKERS IN BREAST CANCER

APPLICATION No. 60/628,101 FILED Nov. 17, 2004—DIFFERENTIAL EXPRESSION OF MARKERS IN BREAST CANCER II

APPLICATION No. 60/620,874 FILED Oct. 22, 2004—DIFFERENTIAL EXPRESSION OF MARKERS IN OVARIAN CANCER

APPLICATION No. 60/628,134 FILED Nov. 17, 2004—DIFFERENTIAL EXPRESSION OF MARKERS IN OVARIAN CANCER II

APPLICATION No. 60/620,924 FILED Oct. 22, 2004—DIFFERENTIAL EXPRESSION OF MARKERS IN STOMACH CANCER

APPLICATION No. 60/628,111 FILED Nov. 17, 2004—DIFFERENTIAL EXPRESSION OF MARKERS IN STOMACH CANCER II

APPLICATION No. 60/620,853 FILED Oct. 22, 2004—28814—DIFFERENTIAL EXPRESSION OF MARKERS IN LUNG CANCER

APPLICATION No. 60/628,112 FILED Nov. 17, 2004—DIFFERENTIAL EXPRESSION OF MARKERS IN LUNG CANCER II

APPLICATION No. 60/620,974 FILED Oct. 22, 2004—DIFFERENTIAL EXPRESSION OF MARKERS IN PANCREATIC CANCER

APPLICATION No. 60/628,145 FILED Nov. 17, 2004—DIFFERENTIAL EXPRESSION OF MARKERS IN PANCREATIC CANCER II

APPLICATION No. 60/620,656 FILED Oct. 22, 2004—DIFFERENTIAL EXPRESSION OF MARKERS IN PROSTATE CANCER

APPLICATION No. 60/628,251 FILED Nov. 17, 2004—DIFFERENTIAL EXPRESSION OF MARKERS IN PROSTATE CANCER II

APPLICATION No. 60/620,975 FILED Oct. 22, 2004—DIFFERENTIAL EXPRESSION OF MARKERS IN BRAIN CANCER

APPLICATION No. 60/628,178 FILED Nov. 17, 2004—DIFFERENTIAL EXPRESSION OF MARKERS IN BRAIN CANCER II

APPLICATION No. 60/622,320 FILED Oct. 27, 2004—DIAGNOSTIC MARKERS FOR CARDIAC DISEASE AND/OR PATHOLOGICAL CONDITIONS, AND ASSAYS AND METHODS OF USE THEREOF

APPLICATION No. 60/628,190 FILED Nov. 17, 2004—DIAGNOSTIC MARKERS FOR CARDIAC DISEASE AND/OR PATHOLOGICAL CONDITIONS, AND ASSAYS AND METHODS OF USE THEREOF II

APPLICATION No. 60/630,559 FILED Nov. 26, 2004—DIAGNOSTIC MARKERS FOR CARDIAC DISEASE AND/OR PATHOLOGICAL CONDITIONS, AND ASSAYS AND METHODS OF USE THEREOF II

APPLICATION No. 60/628,231 FILED Nov. 17, 2004—NOVEL DIAGNOSTIC SERUM MARKERS, AND ASSAYS AND METHODS OF USE THEREOF

APPLICATION No. 60/620,918 FILED Oct. 22, 2004—DIAGNOSTIC MARKERS FOR RENAL CANCER, AND ASSAYS AND METHODS OF USE THEREOF

APPLICATION No. 60/628,156 FILED Nov. 17, 2004—DIAGNOSTIC MARKERS FOR RENAL CANCER, AND ASSAYS AND METHODS OF USE THEREOF II

APPLICATION No. 60/620,677 FILED Oct. 22, 2004—DIFFERENTIAL EXPRESSION OF MARKERS IN BLADDER CANCER

APPLICATION No. 60/628,167 FILED Nov. 17, 2004—DIFFERENTIAL EXPRESSION OF MARKERS IN BLADDER CANCER II

APPLICATION No. 60/621,004 FILED Oct. 22, 2004—DIFFERENTIAL EXPRESSION OF MARKERS IN SKIN AND EPITHELIAL CANCER II

application Ser. No. ----NA---- FILED Nov. 17, 2004—NOVEL DIAGNOSTIC MARKERS, AND ASSAYS AND METHODS OF USE THEREOF

APPLICATION No. 60/622,017 FILED Oct. 27, 2004—VARIANTS OF NONSPECIFIC ALKALINE PHOSPHATASE, USE OF DIAGNOSTIC MARKERS, AND ASSAYS AND METHODS OF USE THEREOF

APPLICATION No. 60/539,129 FILED Jan. 27, 2004—METHODS AND SYSTEMS FOR ANNOTATING BIOMOLECULAR SEQUENCES

APPLICATION No. 60/539,128 FILED Jan. 27, 2004—EVOLUTIONARY CONSERVED SPLICED SEQUENCES AND METHODS AND SYSTEMS FOR IDENTIFYING THEREOF AND application Ser. No. 11/050,857 FILED Jan. 27, 2005

FIELD OF THE INVENTION

The present invention is related to novel nucleotide and protein sequences that are diagnostic markers for ovarian cancer, and assays and methods of use thereof. The “Sequence Listing” recited on the computer readable form (CRF) CD filed herewith is incorporated herein by reference in its entirety.

BACKGROUND OF THE INVENTION

Ovarian cancer causes more deaths than any other cancer of the female reproductive system. An estimated 25,580 new cases will be diagnosed during 2004 in the United States, and approximately 16,090 of these women will die of the disease. Despite advances in the management of advanced ovarian cancer, 70% to 80% of patients will ultimately succumb to disease that is diagnosed in late stages. When ovarian cancer is diagnosed in stage I, more than 90% of patients can be cured with conventional surgery and chemotherapy. At present, however, only 25% of ovarian cancers are detected in stage I. Detection of a greater fraction of ovarian cancers at an early stage might significantly affect survival. A worldwide research effort, aiming at early detection of ovarian cancer, is currently being performed; finding molecular markers for the disease is one of the major research topics (J Clin Oncol. 2003 May 15; 21(10 Suppl):200-5).

No single marker has been shown to be sufficiently sensitive or specific to contribute to the diagnosis of ovarian cancer. The marker that is currently most frequently used is CA-125 (Br J Cancer. 2000 May; 82(9):1535-8). Its properties do not support its use for screening, but it is a major diagnostic tool. CA-125 is a member of the epithelial sialomucins markers group and is the most well documented and the best performing single marker from this group. Another name for CA-125 is mucin 16, and although it is a membrane protein, it can be found in the serum. Its greatest sensitivity is achieved for serous and emdometrioid ovarian tumors compared to mucinous or clear cell tumors. Other than diagnosis, it can be used for disease monitoring (Eur J Gynaecol Oncol. 2000; 21(1):64-9). In about 70% of patients, a rising level of CA-125 may be the first indication of relapse, predating clinical relapse by a median of 4 months. The serum concentration of CA-125 is elevated by the vascular invasion, tissue destruction and inflammation associated with malignant disease and is elevated in over 90% of those women with advanced ovarian cancer. Yet, CA-125 is not specific to ovarian cancer. It is elevated in 40% of all patients with advanced intra-abdominal malignancy. Levels can also be elevated during menstruation or pregnancy and in other benign conditions such as endometriosis, peritonitis or cirrhosis, particularly with ascites. CA-125 is not a marker that can be detected through use of urine samples due to a high molecular weight.

There are other ovarian cancer markers originating from epithelial mucins but none can replace CA-125, due to poorer specificity and sensitivity. These other markers may prove complementary to CA-125. CA-50, CA 54-61, CA-195 and CA 19-9 all appear to have greater sensitivity for detection of mucinous tumors while STN and TAG-72 have better sensitivity for detection of clear cell tumors (Dis Markers. 2004; 20(2):53-70).

Kallikreins, a family of serine proteases, and other protease-related proteins are also potential markers for ovarian cancer. Indeed, the entire family of kallikreins map to a region on chromosome 19q which is shown to be amplified in ovarian cancers. In particular, kallikrein 6 (protease M) and kallilrein 10 have been reported to have sensitivity up to 75% and specificity up to 100%. Matrix metalloproteinases (MMPs) are another family of proteases useful in ovarian cancer screening and prognosis. MMP-2 was reported to have 66% sensitivity and 100% specificity in one study. Cathepsin L, a cystein protease, was described to have a lower false positive rate compared with CA-125. Based on their biochemical proteolytic role, it would seem likely that these proteases would be active in invasion and metastasis formation and indeed these markers appear to have higher sensitivity for advanced stages of the disease. Due to their relatively low molecular weight, such proteases are candidates to be urine markers, or markers which can be detected in urine samples (Dis Markers. 2004; 20(2):53-70).

Hormones have a role in normal ovarian physiology. Therefore, it is not surprising that hormones, and growth and inhibition factors as well, are suitable for ovarian cancer detection. Measurements of fragments of gonadotropin in the urine were found to have sensitivity up to 83% and specificity up to 92% for detecting ovarian cancer. Inhibins, members of the Transforming Growth Factors (TGF) beta superfamily, have been shown to have a diagnostic value in the detection of granulosa cell tumor, a relatively uncommon type of ovarian cancer, associated with better prognosis overall. Serum inhibin is an ovarian product which decreases to non detectable levels after menopause, however, certain ovarian cancers (mucinous carcinomas and sex cord stromal tumours such as granulosa cell tumours) continue to produce inhibin. Studies have shown that that inhibin assays which detect all inhibin forms (as opposed to test detecting specific members of the inhibins family) provide the highest sensitivity/specificity characteristics as an ovarian cancer diagnostic test (Mol Cell Endocrinol. 2002 May 31; 191(1):97-103). Measurement of serum TGF-alpha itself was found to have sensitivity up to 70% and specificity of 89% in early stage disease. The growth factor Mesothelin was also found to have diagnostic value but only for late stage disease.

Immunohistochemistry is frequently used to assess the origin of tumor and staging when a pathological tissue sample is available. A few molecular markers have been shown to have diagnostic value in Immunohistochemistry of ovarian cancer, among them Epidermal Growth Factor, p53 and HER-2. P53 expression is much lower at early stage than late stage disease. P53 high expression is more typical or characteristic of invasive serous tumors than of mucinous tumors. No benign tumors are stained with P53. HER-2 is found in less than 25% of newly diagnosed ovarian cancers. Ovarian cancer of type granulosa cell tumor has in general better prognosis with late relapse and/or metastasis formation. However, about 50% of patients still die within 20 years of diagnosis. In this specific tumor type, immunohistochemistry staining of estrogen receptor beta (ERb) and proliferating cell nuclear antigen (PCNA) showed that loss of ERb expression and high PCNA expression, characterized a subgroup of granulosa cell tumors with a worse outcome (Histopathology. 2003 September; 43(3):254-62). Survivin expression was also shown to be correlated to tumor grade, histologic type and mutant p53 but actual correlation to survival is questionable (Mod Pathol. 2004 February; 17(2):264)

Many other markers have been tested over the years for ovarian cancer detection. Some markers have shown only limited value while others are still under investigation. Among them are TPA and TPS, two cytokeratins whose inclusion in a panel with CA-125 resulted in diagnoses with sensitivity up to 93% and specificity up to 98%. LPA—lysophosphatidic acid—was a very promising marker with one study demonstrating 98% sensitivity and 90% specificity. However, this marker is very unstable and requires quick processing and freezing of plasma, and therefore has limited usage.

As previously described, no single marker has been shown to be sufficiently sensitive or specific to contribute to the diagnosis of ovarian cancer. Therefore combinations of markers in panel are being tested. Usually CA-125 is one of the panel members. The best performing panel combinations so far have been CA-125 with CA 15-3 with sensitivity of 93% and specificity of 93%, CA-125 with CEA (which has very little sensitivity by itself) with specificity of 93% and specificity of 93%, and CA-125 with TAG-72 and CA 15-3 where specificity becomes 95% but sensitivity is diminished (Dis Markers. 2004; 20(2):53-70).

SUMMARY OF THE INVENTION

The background art does not teach or suggest markers for ovarian cancer that are sufficiently sensitive and/or accurate, alone or in combination.

The present invention overcomes these deficiencies of the background art by providing novel markers for ovarian cancer that are both sensitive and accurate. These markers are differentially expressed and preferably overexpressed in ovarian cancer specifically, as opposed to normal ovarian tissue. The measurement of these markers, alone or in combination, in patient (biological) samples provides information that the diagnostician can correlate with a probable diagnosis of ovarian cancer. The markers of the present invention, alone or in combination, show a high degree of differential detection between ovarian cancer and non-cancerous states.

According to preferred embodiments of the present invention, examples of suitable biological samples which may optionally be used with preferred embodiments of the present invention include but are not limited to blood, serum, plasma, blood cells, urine, sputum, saliva, stool, spinal fluid or CSF, lymph fluid, the external secretions of the skin, respiratory, intestinal, and genitourinary tracts, tears, milk, neuronal tissue, ovarian tissue, any human organ or tissue, including any tumor or normal tissue, any sample obtained by lavage (for example of the bronchial system or of the female reproductive system), and also samples of in vivo cell culture constituents. In a preferred embodiment, the biological sample comprises ovarian tissue and/or a serum sample and/or a urine sample and/or secretions or other samples from the female reproductive system and/or any other tissue or liquid sample. The sample can optionally be diluted with a suitable eluant before contacting the sample to an antibody and/or performing any other diagnostic assay.

Information given in the text with regard to cellular localization was determined according to four different software programs: (i) tmhmm (from Center for Biological Sequence Analysis, Technical University of Denmark DTU, cbs.dtu.dk/services/TMHMM/TMHMM2.0b.guide.php) or (ii) tmpred (from EMBnet, maintained by the ISREC Bionformatics group and the LICR Information Technology Office, Ludwig Institute for Cancer Research, Swiss Institute of Bioinformatics, ch.embnet.org/software/TMPRED_form.html) for transmembrane region prediction; (iii) signalp_hmm or (iv) signalp_nn (both from Center for Biological Sequence Analysis, Technical University of Denmark DTU, cbs.dtu.dk/services/SignalP/background/prediction.php) for signal peptide prediction. The terms “signalp_hmm” and “signalp_nn” refer to two modes of operation for the program SignalP: hmm refers to Hidden Markov Model, while nn refers to neural networks. Localization was also determined through manual inspection of known protein localization and/or gene structure, and the use of heuristics by the individual inventor. In some cases for the manual inspection of cellular localization prediction inventors used the ProLoc computational platform [Einat Hazkani-Covo, Erez Levanon, Galit Rotman, Dan Graur and Amit Novik; (2004) “Evolution of multicellularity in metazoa: comparative analysis of the subcellular localization of proteins in Saccharomyces, Drosophila and Caenorhabditis.” Cell Biology International 2004; 28(3):171-8.], which predicts protein localization based on various parameters including, protein domains (e.g., prediction of trans-membranous regions and localization thereof within the protein), pI, protein length, amino acid composition, homology to pre-annotated proteins, recognition of sequence patterns which direct the protein to a certain organelle (such as, nuclear localization signal, NLS, mitochondria localization signal), signal peptide and anchor modeling and using unique domains from Pfam that are specific to a single compartment.

Information is given in the text with regard to SNPs (single nucleotide polymorphisms). A description of the abbreviations is as follows. “T->C”, for example, means that the SNP results in a change at the position given in the table from T to C. Similarly, “M->Q”, for example, means that the SNP has caused a change in the corresponding amino acid sequence, from methionine (M) to glutamine (Q). If, in place of a letter at the right hand side for the nucleotide sequence SNP, there is a space, it indicates that a frameshift has occurred. A frameshift may also be indicated with a hyphen (-). A stop codon is indicated with an asterisk at the right hand side (*). As part of the description of an SNP, a comment may be found in parentheses after the above description of the SNP itself. This comment may include an FTId, which is an identifier to a SwissProt entry that was created with the indicated SNP. An FTId is a unique and stable feature identifier, which allows construction of links directly from position-specific annotation in the feature table to specialized protein-related databases. The FTId is always the last component of a feature in the description field, as follows: FTId=XXX_number, in which XXX is the 3-letter code for the specific feature key, separated by an underscore from a 6-digit number. In the table of the amino acid mutations of the wild type proteins of the selected splice variants of the invention, the header of the first column is “SNP position(s) on amino acid sequence”, representing a position of a known mutation on amino acid sequence. SNPs may optionally be used as diagnostic markers according to the present invention, alone or in combination with one or more other SNPs and/or any other diagnostic marker. Preferred embodiments of the present invention comprise such SNPs, including but not limited to novel SNPs on the known (WT or wild type) protein sequences given below, as well as novel nucleic acid and/or amino acid sequences formed through such SNPs, and/or any SNP on a variant amino acid and/or nucleic acid sequence described herein.

Information given in the text with regard to the Homology to the known proteins was determined by Smith-Waterman version 5.1.2 using special (non default) parameters as follows:

model=sw.model

GAPEXT=0 GAPOP=100.0

MATRIX=blosum100

Information is given with regard to overexpression of a cluster in cancer based on ESTs. A key to the p values with regard to the analysis of such overexpression is as follows:

-   -   library-based statistics: P-value without including the level of         expression in cell-lines (PI)     -   library based statistics: P-value including the level of         expression in cell-lines (P2)     -   EST clone statistics: P-value without including the level of         expression in cell-lines (SP1)     -   EST clone statistics: predicted overexpression ratio without         including the level of expression in cell-lines (R3)     -   EST clone statistics: P-value including the level of expression         in cell-lines (SP2)     -   EST clone statistics: predicted overexpression ratio including         the level of expression in cell-lines (R4)

Library-based statistics refer to statistics over an entire library, while EST clone statistics refer to expression only for ESTs from a particular tissue or cancer.

Information is given with regard to overexpression of a cluster in cancer based on microarrays. As a microarray reference, in the specific segment paragraphs, the unabbreviated tissue name was used as the reference to the type of chip for which expression was measured.

There are two types of microarray results: those from microarrays prepared according to a design by the present inventors, for which the microarray fabrication procedure is described in detail in Materials and Experimental Procedures section herein; and those results from microarrays using Affymetrix technology. As a microarray reference, in the specific segment paragraphs, the unabbreviated tissue name was used as the reference to the type of chip for which expression was measured. For microarrays prepared according to a design by the present inventors, the probe name begins with the name of the cluster (gene), followed by an identifying number. These probes are listed below with their respective sequences.

(SEQ ID NO:1012) CCCCAGCTTTTATAGAGCGGCCCAAGGAAGAATATTTCCAAGAAGTAGGG >HSAPHOL_0_11_0 (SEQ ID NO:1013) GGAACATTCTGGATCTGACCCTCCCAGTCTCATCTCCTGACCCTCCCACT >HUMGRP5E_0_0_16630 (SEQ ID NO:1014) GCTGATATGGAAGTTGGGGAATCTGAATTGCCAGAGAATCTTGGGAAGAG >HUMGRP5E_0_2_0 (SEQ ID NO:1015) TCTCATAGAAGCAAAGGAGAACAGAAACCACCAGCCACCTCAACCCAAGG >D56406_0_5_0 (SEQ ID NO:1016) TCTGACTTTTACGGACTTGGCTTGTTAGAAGGCTGAAAGATGATGGCAGG >M77904_0_8_0 (SEQ ID NO:1017) AGTCTGTGTTTGAGGGTGAAGGCTCAGCAACCCTGATGTCTGCCAACTAC >Z25299_0_3_0 (SEQ ID NO:1018) AACTCTGGCACCTTGGGCTGTGGAAGGCTCTGGAAAGTCCTTCAAAGCTG >Z44808_0_8_0 (SEQ ID NO:1019) AAAAGCATGAGTTTCTGACCAGCGTTCTGGACGCGCTGTCCACGGACATG >Z44808_0_0_72347 (SEQ ID NO:1020) ATGTTCTTAGGAGGCAAGCCAGGAGAAGCCGGGTCTGACTTTTCAGCTCA >Z44808_0_0_72349 (SEQ ID NO:1021) TCCTCCAGACCCAAAGCCACAACCCATCGCAAGTCAAGAACACTTTCCAG >S67314_0_0_741 (SEQ ID NO:1022) CACAGAGCCAGGATGTTCTTCTGACCTCAGTATCTACTCCAGCTCCAGCT >S67314_0_0_744 (SEQ ID NO:1023) TGGCATGCTGGAACATGGACTCTAGCTAGCAAGAAGGGCTCAAGGAGGTG >Z39337_0_0_66755 (SEQ ID NO:1024) GCAGGGGTTAAAAGGACGTTCCAGAAGCATCTGGGGACAGAACCAGCCTC >Z39337_0_9_0 (SEQ ID NO:1025) TAATAAACGCAGCGACGTGAGGGTCCTGATTCTCCCTGGTTTTACCCCAG >HUMPHOSLIP_0_0_18458 (SEQ ID NO:1026) AAGGAAGCAGGACCAGTGGATGTGAGGCGTGGTCGAAGAACAACAGAAAG >HUMPHOSLIP_0_0_18487 (SEQ ID NO:1027) ACAGGGGCCAGATGGTGACCCATGACCCAGCCTAAAAGGCAGCCAGAGGG >M78530_0_6_0 (SEQ ID NO:1028) CTTCCTACACACATCTAGACGTTCAAGTTTGCAAATCAGTTTTTAGCAAG >HSMUC1A_0_37_0 (SEQ ID NO:1029) AAAAGGAGACTTCGGCTACCCAGAGAAGTTCAGTGCCCAGCTCTACTGAG >HSMUC1A_0_0_11364 (SEQ ID NO:1030) AAAGGCTGGCATAGGGGGAGGTTTCCCAGGTAGAAGAAGAAGTGTCAGCA >HSMUC1A_0_0_11365 (SEQ ID NO:1031) AATTAACCCTTTGAGAGCTGGCCAGGACTCTGGACTGATTACCCCAGCCT >H61775_011_0

Oligonucleotide microarray results taken from Affymetrix data were from chips available from Affymetrix Inc, Santa Clara, Calif., USA (see for example data regarding the Human Genome U133 (HG-U133) Set at www.affymetrix.com/products/arrays/specific/hgu133.affx; GeneChip Human Genome U133A 2.0 Array at www.affymetrix.com/products/arrays/specific/hgu133av2.affx; and Human Genome U133 Plus 2.0 Array at www.affymetrix.com/products/arrays/specific/hgu133plus.affx). The probe names follow the Affymetrix naming convention. The data is available from NCBI Gene Expression Omnibus (see www.ncbi.nlm.nih.gov/projects/geo/ and Edgar et al., Nucleic Acids Research, 2002, Vol. 30, No. 1 207-210). The dataset (including results) is available from www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE1133 for the Series GSE1133 database (published on March 2004); a reference to these results is as follows: Su et al. (Proc Natl Acad Sci USA. 2004 Apr. 20; 101(16):6062-7. Epub 2004 Apr. 9).

The following list of abbreviations for tissues was used in the TAA histograms. The term “TAA” stands for “Tumor Associated Antigen”, and the TAA histograms, given in the text, represent the cancerous tissue expression pattern as predicted by the biomarkers selection engine, as described in detail in examples 1-5 below (the first word is the abbreviation while the second word is the full name):

(“BONE”, “bone”); (“COL”, “colon”); (“EPI”, “epithelial”); (“GEN”, “general”); (“LIVER”, “liver”); (“LUN”, “lung”); (“LYMPH”, “lymph nodes”); (“MARROW”, “bone marrow”); (“OVA”, “ovary”); (“PANCREAS”, “pancreas”); (“PRO”, “prostate”); (“STOMACH”, “stomach”); (“TCELL”, “T cells”);

(“THYROID”, “Thyroid”);

(“MAM”, “breast”); (“BRAIN”, “brain”); (“UTERUS”, “uterus”); (“SKIN”, “skin”); (“KIDNEY”, “kidney”); (“MUSCLE”, “muscle”); (“ADREN”, “adrenal”); (“HEAD”, “head and neck”); (“BLADDER”, “bladder”);

It should be noted that the terms “segment”, “seg” and “node” are used interchangeably in reference to nucleic acid sequences of the present invention; they refer to portions of nucleic acid sequences that were shown to have one or more properties as described below. They are also the building blocks that were used to construct complete nucleic acid sequences as described in greater detail below. Optionally and preferably, they are examples of oligonucleotides which are embodiments of the present invention, for example as amplicons, hybridization units and/or from which primers and/or complementary oligonucleotides may optionally be derived, and/or for any other use.

Unless defined otherwise, all technical and scientific terms used herein have the meaning commonly understood by a person skilled in the art to which this invention belongs. The following references provide one of skill with a general definition of many of the terms used in this invention: Singleton et al., Dictionary of Microbiology and Molecular Biology (2nd ed. 1994); The Cambridge Dictionary of Science and Technology (Walker ed., 1988); The Glossary of Genetics, 5th Ed., R. Rieger et al. (eds.), Springer Verlag (1991); and Hale & Marham, The Harper Collins Dictionary of Biology (1991). All of these are hereby incorporated by reference as if fully set forth herein. As used herein, the following terms have the meanings ascribed to them unless specified otherwise.

As used herein the phrase “ovarian cancer” refers to cancers of the ovary including but not limited to Ovarian epithelial tumors (serous, mucinous, endometroid, clear cell, and Brenner tumor), ovarian germ-cell tumors, (teratoma, dysgerminoma, endodermal sinus tumor, and embryonal carcinoma) and ovarian stromal tumors (originating from granulosa, theca, Sertoli, Leydig, and collagen-producing stromal cells).

The term “marker” in the context of the present invention refers to a nucleic acid fragment, a peptide, or a polypeptide, which is differentially present in a sample taken from subjects (patients) having ovarian cancer as compared to a comparable sample taken from subjects who do not have ovarian cancer.

The phrase “differentially present” refers to differences in the quantity of a marker present in a sample taken from patients having ovarian cancer as compared to a comparable sample taken from patients who do not have ovarian cancer. For example, a nucleic acid fragment may optionally be differentially present between the two samples if the amount of the nucleic acid fragment in one sample is significantly different from the amount of the nucleic acid fragment in the other sample, for example as measured by hybridization and/or NAT-based assays. A polypeptide is differentially present between the two samples if the amount of the polypeptide in one sample is significantly different from the amount of the polypeptide in the other sample. It should be noted that if the marker is detectable in one sample and not detectable in the other, then such a marker can be considered to be differentially present.

As used herein the phrase “diagnostic” means identifying the presence or nature of a pathologic condition. Diagnostic methods differ in their sensitivity and specificity. The “sensitivity” of a diagnostic assay is the percentage of diseased individuals who test positive (percent of “true positives”). Diseased individuals not detected by the assay are “false negatives.” Subjects who are not diseased and who test negative in the assay are termed “true negatives.” The “specificity” of a diagnostic assay is 1 minus the false positive rate, where the “false positive” rate is defined as the proportion of those without the disease who test positive. While a particular diagnostic method may not provide a definitive diagnosis of a condition, it suffices if the method provides a positive indication that aids in diagnosis.

As used herein the phrase “diagnosing” refers to classifying a disease or a symptom, determining a severity of the disease, monitoring disease progression, forecasting an outcome of a disease and/or prospects of recovery. The term “detecting” may also optionally encompass any of the above.

Diagnosis of a disease according to the present invention can be effected by determining a level of a polynucleotide or a polypeptide of the present invention in a biological sample obtained from the subject, wherein the level determined can be correlated with predisposition to, or presence or absence of the disease. It should be noted that a “biological sample obtained from the subject” may also optionally comprise a sample that has not been physically removed from the subject, as described in greater detail below.

As used herein, the term “level” refers to expression levels of RNA and/or protein or to DNA copy number of a marker of the present invention.

Typically the level of the marker in a biological sample obtained from the subject is different (i.e., increased or decreased) from the level of the same variant in a similar sample obtained from a healthy individual (examples of biological samples are described herein).

Numerous well known tissue or fluid collection methods can be utilized to collect the biological sample from the subject in order to determine the level of DNA, RNA and/or polypeptide of the variant of interest in the subject.

Examples include, but are not limited to, fine needle biopsy, needle biopsy, core needle biopsy and surgical biopsy (e.g., brain biopsy), and lavage. Regardless of the procedure employed, once a biopsy/sample is obtained the level of the variant can be determined and a diagnosis can thus be made.

Determining the level of the same variant in normal tissues of the same origin is preferably effected along-side to detect an elevated expression and/or amplification and/or a decreased expression, of the variant as opposed to the normal tissues.

A “test amount” of a marker refers to an amount of a marker in a subject's sample that is consistent with a diagnosis of ovarian cancer. A test amount can be either in absolute amount (e.g., microgram/ml) or a relative amount (e.g., relative intensity of signals).

A “control amount” of a marker can be any amount or a range of amounts to be compared against a test amount of a marker. For example, a control amount of a marker can be the amount of a marker in a patient with ovarian cancer or a person without ovarian cancer. A control amount can be either in absolute amount (e.g., microgram/ml) or a relative amount (e.g., relative intensity of signals).

“Detect” refers to identifying the presence, absence or amount of the object to be detected.

A “label” includes any moiety or item detectable by spectroscopic, photo chemical, biochemical, immunochemical, or chemical means. For example, useful labels include ³²P, ³⁵S, fluorescent dyes, electron-dense reagents, enzymes (e.g., as commonly used in an ELISA), biotin-streptavadin, dioxigenin, haptens and proteins for which antisera or monoclonal antibodies are available, or nucleic acid molecules with a sequence complementary to a target. The label often generates a measurable signal, such as a radioactive, chromogenic, or fluorescent signal, that can be used to quantify the amount of bound label in a sample. The label can be incorporated in or attached to a primer or probe either covalently, or through ionic, van der Waals or hydrogen bonds, e.g., incorporation of radioactive nucleotides, or biotinylated nucleotides that are recognized by streptavadin. The label may be directly or indirectly detectable. Indirect detection can involve the binding of a second label to the first label, directly or indirectly. For example, the label can be the ligand of a binding partner, such as biotin, which is a binding partner for streptavadin, or a nucleotide sequence, which is the binding partner for a complementary sequence, to which it can specifically hybridize. The binding partner may itself be directly detectable, for example, an antibody may be itself labeled with a fluorescent molecule. The binding partner also may be indirectly detectable, for example, a nucleic acid having a complementary nucleotide sequence can be a part of a branched DNA molecule that is in turn detectable through hybridization with other labeled nucleic acid molecules (see, e.g., P. D. Fahrlander and A. Klausner, Bio/Technology 6:1165 (1988)). Quantitation of the signal is achieved by, e.g., scintillation counting, densitometry, or flow cytometry.

Exemplary detectable labels, optionally and preferably for use with immunoassays, include but are not limited to magnetic beads, fluorescent dyes, radiolabels, enzymes (e.g., horse radish peroxide, alkaline phosphatase and others commonly used in an ELISA), and calorimetric labels such as colloidal gold or colored glass or plastic beads. Alternatively, the marker in the sample can be detected using an indirect assay, wherein, for example, a second, labeled antibody is used to detect bound marker-specific antibody, and/or in a competition or inhibition assay wherein, for example, a monoclonal antibody which binds to a distinct epitope of the marker are incubated simultaneously with the mixture.

“Immunoassay” is an assay that uses an antibody to specifically bind an antigen. The immunoassay is characterized by the use of specific binding properties of a particular antibody to isolate, target, and/or quantify the antigen.

The phrase “specifically (or selectively) binds” to an antibody or “specifically (or selectively) immunoreactive with,” when referring to a protein or peptide (or other epitope), refers to a binding reaction that is determinative of the presence of the protein in a heterogeneous population of proteins and other biologics. Thus, under designated immunoassay conditions, the specified antibodies bind to a particular protein at least two times greater than the background (non-specific signal) and do not substantially bind in a significant amount to other proteins present in the sample. Specific binding to an antibody under such conditions may require an antibody that is selected for its specificity for a particular protein. For example, polyclonal antibodies raised to seminal basic protein from specific species such as rat, mouse, or human can be selected to obtain only those polyclonal antibodies that are specifically immunoreactive with seminal basic protein and not with other proteins, except for polymorphic variants and alleles of seminal basic protein. This selection may be achieved by subtracting out antibodies that cross-react with seminal basic protein molecules from other species. A variety of immunoassay formats may be used to select antibodies specifically immunoreactive with a particular protein. For example, solid-phase ELISA immunoassays are routinely used to select antibodies specifically immunoreactive with a protein (see, e.g., Harlow & Lane, Antibodies, A Laboratory Manual (1988), for a description of immunoassay formats and conditions that can be used to determine specific immunoreactivity). Typically a specific or selective reaction will be at least twice background signal or noise and more typically more than 10 to 100 times background.

According to preferred embodiments of the present invention, there is provided an isolated polynucleotide comprising a nucleic acid sequence in the table below and/or:

Transcript Name H61775_T21 (SEQ ID NO.1) H61775_T22 (SEQ ID NO:2) a nucleic acid sequence comprising a sequence in the table below:

Segment Name H61775_node_2 (SEQ ID NO:3) H61775_node_4 (SEQ ID NO:4) H61775_node_6 (SEQ ID NO:5) H61775_node_8 (SEQ ID NO:6) H61775_node_0 (SEQ ID NO:7) H61775_node_5 (SEQ ID NO:8)

According to preferred embodiments of the present invention, there is provided an isolated polypeptide comprising an amino acid sequence in the table below amino acid sequence comprising a sequence in the table below:

Protein Name H61775_P16 (SEQ ID NO:9) H61775_P17 (SEQ ID NO:10)

According to preferred embodiments of the present invention, there is provided an isolated polynucleotide comprising a nucleic acid sequence in the table below and/or:

Transcript Name HUMCEA_PEA_1_T8 (SEQ ID NO:502) HUMCEA_PEA_1_T9 (SEQ ID NO:503) HUMCEA_PEA_1_T20 (SEQ ID NO:504) HUMCEA_PEA_1_T25 (SEQ ID NO:505) HUMCEA_PEA_1_T26 (SEQ ID NO:506) a nucleic acid sequence comprising a sequence in the table below:

Segment Name HUMCEA_PEA_1_node_0 (SEQ ID NO:507) HUMCEA_PEA_1_node_2 (SEQ ID NO:508) HUMCEA_PEA_1_node_11 (SEQ ID NO:509) HUMCEA_PEA_1_node_12 (SEQ ID NO:510) HUMCEA_PEA_1_node_31 (SEQ ID NO:511) HUMCEA_PEA_1_node_36 (SEQ ID NO:512) HUMCEA_PEA_1_node_44 (SEQ ID NO:513) HUMCEA_PEA_1_node_46 (SEQ ID NO:514) HUMCEA_PEA_1_node_63 (SEQ ID NO:515) HUMCEA_PEA_1_node_65 (SEQ ID NO:516) HUMCEA_PEA_1_node_67 (SEQ ID NO:517) HUMCEA_PEA_1_node_3 (SEQ ID NO:518) HUMCEA_PEA_1_node_7 (SEQ ID NO:519) HUMCEA_PEA_1_node_8 (SEQ ID NO:520) HUMCEA_PEA_1_node_9 (SEQ ID NO:521) HUMCEA_PEA_1_node_10 (SEQ ID NO:522) HUMCEA_PEA_1_node_15 (SEQ ID NO:523) HUMCEA_PEA_1_node_16 (SEQ ID NO:524) HUMCEA_PEA_1_node_17 (SEQ ID NO:525) HUMCEA_PEA_1_node_18 (SEQ ID NO:526) HUMCEA_PEA_1_node_19 (SEQ ID NO:527) HUMCEA_PEA_1_node_20 (SEQ ID NO:528) HUMCEA_PEA_1_node_21 (SEQ ID NO:529) HUMCEA_PEA_1_node_22 (SEQ ID NO:530) HUMCEA_PEA_1_node_23 (SEQ ID NO:531) HUMCEA_PEA_1_node_24 (SEQ ID NO:532) HUMCEA_PEA_1_node_27 (SEQ ID NO:533) HUMCEA_PEA_1_node_29 (SEQ ID NO:534) HUMCEA_PEA_1_node_30 (SEQ ID NO:535) HUMCEA_PEA_1_node_33 (SEQ ID NO:536) HUMCEA_PEA_1_node_34 (SEQ ID NO:537) HUMCEA_PEA_1_node_35 (SEQ ID NO:538) HUMCEA_PEA_1_node_45 (SEQ ID NO:539) HUMCEA_PEA_1_node_50 (SEQ ID NO:540) HUMCEA_PEA_1_node_51 (SEQ ID NO:541) HUMCEA_PEA_1_node_56 (SEQ ID NO:542) HUMCEA_PEA_1_node_57 (SEQ ID NO:543) HUMCEA_PEA_1_node_58 (SEQ ID NO:544) HUMCEA_PEA_1_node_60 (SEQ ID NO:545) HUMCEA_PEA_1_node_61 (SEQ ID NO:546) HUMCEA_PEA_1_node_62 (SEQ ID NO:547) HUMCEA_PEA_1_node_64 (SEQ ID NO:548)

According to preferred embodiments of the present invention, there is provided an isolated polypeptide comprising an amino acid sequence in the table below:

Corresponding Protein Name Transcript(s) HUMCEA_PEA_1_P4 HUMCEA_PEA_1_T8 (SEQ ID NO:550) (SEQ ID NO:502) HUMCEA_PEA_1_P5 HUMCEA_PEA_1_T9 (SEQ ID NO:551) (SEQ ID NO:503) HUMCEA_PEA_1_P14 HUMCEA_PEA_1_T20 (SEQ ID NO:552) (SEQ ID NO:504) HUMCEA_PEA_1_P19 HUMCEA_PEA_1_T25 (SEQ ID NO:553) (SEQ ID NO:505) HUMCEA_PEA_1_P20 HUMCEA_PEA_1_T26 (SEQ ID NO:554) (SEQ ID NO:506)

According to preferred embodiments of the present invention, there is provided an isolated polynucleotide comprising a nucleic acid sequence in the table below and/or:

Transcript Name HUMEDF_PEA_2_T5 (SEQ ID NO:555) HUMEDF_PEA_2_T10 (SEQ ID NO:556) HUMEDF_PEA_2_T11 (SEQ ID NO:557) a nucleic acid sequence comprising a sequence in the table below:

Segment Name HUMEDF_PEA_2_node_6 (SEQ ID NO: 558) HUMEDF_PEA_2_node_11 (SEQ ID NO: 559) HUMEDF_PEA_2_node_18 (SEQ ID NO: 560) HUMEDF_PEA_2_node_19 (SEQ ID NO: 561) HUMEDF_PEA_2_node_22 (SEQ ID NO: 562) HUMEDF_PEA_2_node_2 (SEQ ID NO: 563) HUMEDF_PEA_2_node_8 (SEQ ID NO: 564) HUMEDF_PEA_2_node_20 (SEQ ID NO: 565)

According to preferred embodiments of the present invention, there is provided an isolated polypeptide comprising an amino acid sequence in the table below:

Corresponding Protein Name Transcript(s) HUMEDF_PEA_2_P5 HUMEDF_PEA_2_T10 (SEQ ID NO:567) (SEQ ID NO:556) HUMEDF_PEA_2_P6 HUMEDF_PEA_2_T11 (SEQ ID NO:568) (SEQ ID NO:557) HUMEDF_PEA_2_P8 HUMEDF_PEA_2_T5 (SEQ ID NO:569) (SEQ ID NO:555)

According to preferred embodiments of the present invention, there is provided an isolated polynucleotide comprising a nucleic acid sequence in the table below and/or:

Transcript Name HSAPHOL_T10 (SEQ ID NO:11) HSAPHOL_T4 (SEQ ID NO:12) HSAPHOL_T5 (SEQ ID NO:13) HSAPHOL_T6 (SEQ ID NO:14) HSAPHOL_T7 (SEQ ID NO:15) HSAPHOL_T8 (SEQ ID NO:16) HSAPHOL_T9 (SEQ ID NO:17) a nucleic acid sequence comprising a sequence in the table below:

Segment Name HSAPHOL_node_11 (SEQ ID NO: 18) HSAPHOL_node_13 (SEQ ID NO: 19) HSAPHOL_node_15 (SEQ ID NO: 20) HSAPHOL_node_19 (SEQ ID NO: 21) HSAPHOL_node_2 (SEQ ID NO: 22) HSAPHOL_node_21 (SEQ ID NO: 23) HSAPHOL_node_23 (SEQ ID NO: 24) HSAPHOL_node_26 (SEQ ID NO: 25) HSAPHOL_node_28 (SEQ ID NO: 26) HSAPHOL_node_38 (SEQ ID NO: 27) HSAPHOL_node_40 (SEQ TD NO: 28) HSAPHOL_node_42 (SEQ ID NO: 29) HSAPHOL_node_16 (SEQ ID NO: 30) HSAPHOL_node_25 (SEQ ID NO: 31) HSAPHOL_node_34 (SEQ ID NO: 32) HSAPHOL_node_35 (SEQ ID NO: 33) HSAPHOL_node_36 (SEQ ID NO: 34) HSAPHOL_node_41 (SEQ ID NO: 35)

According to preferred embodiments of the present invention, there is provided an isolated polypeptide comprising an amino acid sequence in the table below:

Protein Name HSAPHOL_P2 (SEQ ID NO:37) HSAPHOL_P3 (SEQ ID NO:38) HSAPHOL_P4 (SEQ ID NO:39) HSAPHOL_P5 (SEQ ID NO:40) HSAPHOL_P6 (SEQ ID NO:41) HSAPHOL_P7 (SEQ ID NO:42) HSAPHOL_P8 (SEQ ID NO:43)

According to preferred embodiments of the present invention, there is provided an isolated polynucleotide comprising a nucleic acid sequence in the table below and/or:

Transcript Name T10888_PEA_1_T1 (SEQ ID NO:44) T10888_PEA_1_T4 (SEQ ID NO:45) T10888_PEA_1_T5 (SEQ ID NO:46) T10888_PEA_1_T6 (SEQ ID NO:47) a nucleic acid sequence comprising a sequence in the table below:

Segment Name T10888_PEA_1_node_11 (SEQ ID NO:48) T10888_PEA_1_node_12 (SEQ ID NO:49) T10888_PEA_1_node_17 (SEQ ID NO:50) T10888_PEA_1_node_4 (SEQ ID NO:51) T10888_PEA_1_node_6 (SEQ ID NO:52) T10888_PEA_1_node_7 (SEQ ID NO:53) T10888_PEA_1_node_9 (SEQ ID NO:54) T10888_PEA_1_node_15 (SEQ ID NO:55)

According to preferred embodiments of the present invention, there is provided an isolated polypeptide comprising an amino acid sequence in the table below:

Protein Name T10888_PEA_1_P2 (SEQ ID NO:57) T10888_PEA_1_P4 (SEQ ID NO:58) T10888_PEA_1_P5 (SEQ ID NO:59) T10888_PEA_1_P6 (SEQ ID NO:60)

According to preferred embodiments of the present invention, there is provided an isolated polynucleotide comprising a nucleic acid sequence in the table below and/or:

Transcript Name HSECADH_T11 (SEQ ID NO:61) HSECADH_T18 (SEQ ID NO:62) HSECADH_T19 (SEQ ID NO:63) HSECADH_T20 (SEQ ID NO:64) a nucleic acid sequence comprising a sequence in the table below:

Segment Name HSECADH_node_0 (SEQ ID NO:65) HSECADH_node_14 (SEQ ID NO:66) HSECADH_node_15 (SEQ ID NO:67) HSECADH_node_21 (SEQ ID NO:68) HSECADH_node_22 (SEQ ID NO:69) HSECADH_node_25 (SEQ ID NO:70) HSECADH_node_26 (SEQ ID NO:71) HSECADH_node_48 (SEQ ID NO:72) HSECADH_node_52 (SEQ ID NO:73) HSECADH_node_53 (SEQ ID NO:74) HSECADH_node_54 (SEQ ID NO:75) HSECADH_node_57 (SEQ ID NO:76) HSECADH_node_60 (SEQ ID NO:77) HSECADH_node_62 (SEQ ID NO:78) HSECADH_node_63 (SEQ ID NO:79) HSECADH_node_7 (SEQ ID NO:80) HSECADH_node_1 (SEQ ID NO:81) HSECADH_node_11 (SEQ ID NO:82) HSECADH_node_12 (SEQ ID NO:83) HSECADH_node_17 (SEQ ID NO:84) HSECADH_node_18 (SEQ ID NO:85) HSECADH_node_19 (SEQ ID NO:86) HSECADH_node_3 (SEQ ID NO:87) HSECADH_node_42 (SEQ ID NO:88) HSECADH_node_45 (SEQ ID NO:89) HSECADH_node_46 (SEQ ID NO:90) HSECADH_node_55 (SEQ ID NO:91) HSECADH_node_56 (SEQ ID NO:92) HSECADH_node_58 (SEQ ID NO:93) HSECADH_node_59 (SEQ ID NO:94)

According to preferred embodiments of the present invention, there is provided an isolated polypeptide comprising an amino acid sequence in the table below:

Protein Name HSECADH_P9 (SEQ ID NO:96) HSECADH_P13 (SEQ ID NO:97) HSECADH_P14 (SEQ ID NO:98) HSECADH_P15 (SEQ ID NO:99)

According to preferred embodiments of the present invention, there is provided an isolated polynucleotide comprising a nucleic acid sequence in the table below and/or:

Transcript Name HUMGRP5E_T4 (SEQ ID NO:100) HUMGRP5E_T5 (SEQ ID NO:101) a nucleic acid sequence comprising a sequence in the table below:

Segment Name HUMGRP5E_node_0 (SEQ ID NO:102) HUMGRP5E_node_2 (SEQ ID NO:103) HUMGRP5E_node_8 (SEQ ID NO:104) HUMGRP5E_node_3 (SEQ ID NO:105) HUMGRP5E_node_7 (SEQ ID NO:106)

According to preferred embodiments of the present invention, there is provided an isolated polypeptide comprising an amino acid sequence in the table below:

Protein Name HUMGRP5E_P4 (SEQ ID NO:108) HUMGRP5E_P5 (SEQ ID NO:109)

According to preferred embodiments of the present invention, there is provided an isolated polynucleotide comprising a nucleic acid sequence in the table below and/or:

Transcript Name R11723_PEA_1_T15 (SEQ ID NO:110) R11723_PEA_1_T17 (SEQ ID NO:111) R11723_PEA_1_T19 (SEQ ID NO:112) R11723_PEA_1_T20 (SEQ ID NO:113) R11723_PEA_1_T5 (SEQ ID NO:114) R11723_PEA_1_T6 (SEQ ID NO:115) a nucleic acid sequence comprising a sequence in the table below:

Segment Name R11723_PEA_1_node_13 (SEQ ID NO: 116) R11723_PEA_1_node_16 (SEQ ID NO: 117) R11723_PEA_1_node_19 (SEQ ID NO: 118) R11723_PEA_1_node_2 (SEQ ID NO: 119) R11723_PEA_1_node_22 (SEQ ID NO: 120) R11723_PEA_1_node_31 (SEQ ID NO: 121) R11723_PEA_1_node_10 (SEQ ID NO. 122) R11723_PEA_1_node_11 (SEQ ID NO: 123) R11723_PEA_1_node_15 (SEQ ID NO: 124) R11723_PEA_1_node_18 (SEQ ID NO: 125) R11723_PEA_1_node_20 (SEQ ID NO: 126) R11723_PEA_1_node_21 (SEQ ID NO: 127) R11723_PEA_1_node_23 (SEQ ID NO: 128) R11723_PEA_1_node_24 (SEQ ID NO: 129) R11723_PEA_1_node_25 (SEQ ID NO: 130) R11723_PEA_1_node_26 (SEQ ID NO: 131) R11723_PEA_1_node_27 (SEQ ID NO: 132) R11723_PEA_1_node_28 (SEQ ID NO: 133) R11723_PEA_1_node_29 (SEQ ID NO: 134) R11723_PEA_1_node_3 (SEQ ID NO: 135) R11723_PEA_1_node_30 (SEQ ID NO: 136) R11723_PEA_1_node_4 (SEQ ID NO: 137) R11723_PEA_1_node_5 (SEQ ID NO: 138) R11723_PEA_1_node_6 (SEQ ID NO: 139) R11723_PEA_1_node_7 (SEQ ID NO: 140) R11723_PEA_1_node_8 (SEQ ID NO: 141)

According to preferred embodiments of the present invention, there is provided an isolated polypeptide comprising an amino acid sequence in the table below:

Protein Name R11723_PEA_1_P2 (SEQ ID NO: 142) R11723_PEA_1_P6 (SEQ ID NO: 143) R11723_PEA_1_P7 (SEQ ID NO: 144) R11723_PEA_1_P13 (SEQ ID NO: 145) R11723_PEA_1_P10 (SEQ ID NO: 146)

According to preferred embodiments of the present invention, there is provided an isolated polynucleotide comprising a nucleic acid sequence in the table below and/or:

Transcript Name D56406_PEA_1_T3 (SEQ ID NO: 147) D56406_PEA_1_T6 (SEQ ID NO: 148) D56406_PEA_1_T7 (SEQ ID NO: 149) a nucleic acid sequence comprising a sequence in the table below:

Segment Name D56406_PEA_1_node_0 (SEQ ID NO: 150) D56406_PEA_1_node_13 (SEQ ID NO: 151) D56406_PEA_1_node_11 (SEQ ID NO: 152) D56406_PEA_1_node_2 (SEQ ID NO: 153) D56406_PEA_1_node_3 (SEQ ID NO: 154) D56406_PEA_1_node_5 (SEQ ID NO: 155) D56406_PEA_1_node_6 (SEQ ID NO: 156) D56406_PEA_1_node_7 (SEQ ID NO: 157) D56406_PEA_1_node_8 (SEQ ID NO: 158) D56406_PEA_1_node_9 (SEQ ID NO: 159)

According to preferred embodiments of the present invention, there is provided an isolated polypeptide comprising an amino acid sequence in the table below:

Protein Name D56406_PEA_1_P2 (SEQ ID NO: 161) D56406_PEA_1_P5 (SEQ ID NO: 162) D56406_PEA_1_P6 (SEQ ID NO: 163)

According to preferred embodiments of the present invention, there is provided an isolated polynucleotide comprising a nucleic acid sequence in the table below and/or:

Transcript Name H53393_PEA_1_T10 (SEQ ID NO: 164) H53393_PEA_1_T11 (SEQ ID NO: 165) H53393_PEA_1_T3 (SEQ ID NO: 166) H53393_PEA_1_T9 (SEQ ID NO: 167) a nucleic acid sequence comprising a sequence in the table below:

Segment Name H53393_PEA_1_node_0 (SEQ ID NO: 168) H53393_PEA_1_node_10 (SEQ ID NO: 169) H53393_PEA_1_node_12 (SEQ ID NO: 170) H53393_PEA_1_node_13 (SEQ ID NO: 171) H53393_PEA_1_node_15 (SEQ ID NO: 172) H53393_PEA_1_node_17 (SEQ ID NO: 173) H53393_PEA_1_node_19 (SEQ ID NO: 174) H53393_PEA_1_node_23 (SEQ ID NO: 175) H53393_PEA_1_node_24 (SEQ ID NO: 176) H53393_PEA_1_node_25 (SEQ ID NO: 177) H53393_PEA_1_node_29 (SEQ ID NO: 178) H53393_PEA_1_node_4 (SEQ ID NO: 179) H53393_PEA_1_node_6 (SEQ ID NO: 180) H53393_PEA_1_node_8 (SEQ ID NO: 181) H53393_PEA_1_node_21 (SEQ ID NO: 182) H53393_PEA_1_node_22 (SEQ ID NO: 183)

According to preferred embodiments of the present invention, there is provided an isolated polypeptide comprising an amino acid sequence in the table below:

Protein Name H53393_PEA_1_P2 (SEQ ID NO: 185) H53393_PEA_1_P3 (SEQ ID NO: 186) H53393_PEA_1_P6 (SEQ ID NO: 187)

According to preferred embodiments of the present invention, there is provided an isolated polynucleotide comprising a nucleic acid sequence in the table below and/or:

Transcript Name HSU40434_PEA_1_T13 (SEQ ID NO: 188) a nucleic acid sequence comprising a sequence in the table below:

Segment Name HSU40434_PEA_1_node_1 (SEQ ID NO: 189) HSU40434_PEA_1_node_16 (SEQ ID NO: 190) HSU40434_PEA_1_node_30 (SEQ ID NO: 191) HSU40434_PEA_1_node_32 (SEQ ID NO: 192) HSU40434_PEA_1_node_57 (SEQ ID NO: 193) HSU40434_PEA_1_node_0 (SEQ ID NO: 194) HSU40434_PEA_1_node_10 (SEQ ID NO: 195) HSU40434_PEA_1_node_13 (SEQ ID NO: 196) HSU40434_PEA_1_node_18 (SEQ ID NO: 197) HSU40434_PEA_1_node_2 (SEQ ID NO: 198) HSU40434_PEA_1_node_20 (SEQ ID NO: 199) HSU40434_PEA_1_node_21 (SEQ ID NO: 200) HSU40434_PEA_1_node_23 (SEQ ID NO: 201) HSU40434_PEA_1_node_24 (SEQ ID NO: 202) HSU40434_PEA_1_node_26 (SEQ ID NO: 203) HSU40434_PEA_1_node_28 (SEQ ID NO: 204) HSU40434_PEA_1_node_3 (SEQ ID NO: 205) HSU40434_PEA_1_node_35 (SEQ ID NO: 206) HSU40434_PEA_1_node_36 (SEQ ID NO: 207) HSU40434_PEA_1_node_37 (SEQ ID NO: 208) HSU40434_PEA_1_node_38 (SEQ ID NO: 209) HSU40434_PEA_1_node_39 (SEQ ID NO: 210) HSU40434_PEA_1_node_40 (SEQ ID NO: 211) HSU40434_PEA_1_node_41 (SEQ ID NO: 212) HSU40434_PEA_1_node_42 (SEQ ID NO: 213) HSU40434_PEA_1_node_43 (SEQ ID NO: 214) HSU40434_PEA_1_node_44 (SEQ ID NO: 215) HSU40434_PEA_1_node_47 (SEQ ID NO: 216) HSU40434_PEA_1_node_48 (SEQ ID NO: 217) HSU40434_PEA_1_node_51 (SEQ ID NO: 218) HSU40434_PEA_1_node_52 (SEQ ID NO: 219) HSU40434_PEA_1_node_53 (SEQ ID NO: 220) HSU40434_PEA_1_node_54 (SEQ ID NO: 221) HSU40434_PEA_1_node_56 (SEQ ID NO: 222) HSU40434_PEA_1_node_7 (SEQ ID NO: 223) HSU40434_PEA_1_node_8 (SEQ ID NO: 224)

According to preferred embodiments of the present invention, there is provided an isolated polypeptide comprising an amino acid sequence in the table below:

Protein Name HSU40434_PEA_1_P12 (SEQ ID NO: 226)

According to preferred embodiments of the present invention, there is provided an isolated polynucleotide comprising a nucleic acid sequence in the table below and/or:

Transcript Name M77904_T11 (SEQ ID NO: 227) M77904_T3 (SEQ ID NO: 228) M77904_T8 (SEQ ID NO: 229) M77904_T9 (SEQ ID NO: 230) a nucleic acid sequence comprising a sequence in the table below:

Segment Name M77904_node_0 (SEQ ID NO: 231) M77904_node_11 (SEQ ID NO: 232) M77904_node_12 (SEQ ID NO: 233) M77904_node_14 (SEQ ID NO: 234) M77904_node_15 (SEQ ID NO: 235) M77904_node_17 (SEQ ID NO: 236) M77904_node_2 (SEQ ID NO: 237) M77904_node_21 (SEQ ID NO: 238) M77904_node_23 (SEQ ID NO: 239) M77904_node_24 (SEQ ID NO: 240) M77904_node_27 (SEQ ID NO: 241) M77904_node_28 (SEQ ID NO: 242) M77904_node_4 (SEQ ID NO: 243) M77904_node_6 (SEQ ID NO: 244) M77904_node_7 (SEQ ID NO: 245) M77904_node_8 (SEQ ID NO: 246) M77904_node_9 (SEQ ID NO: 247) M77904_node_19 (SEQ ID NO: 248) M77904_node_22 (SEQ ID NO: 249) M77904_node_25 (SEQ ID NO: 250) M77904_node_26 (SEQ ID NO: 251)

According to preferred embodiments of the present invention, there is provided an isolated polypeptide comprising an amino acid sequence in the table below:

Protein Name M77904_P2 (SEQ ID NO: 252) M77904_P4 (SEQ ID NO: 253) M77904_P5 (SEQ ID NO: 254) M77904_P7 (SEQ ID NO: 255)

According to preferred embodiments of the present invention, there is provided an isolated polynucleotide comprising a nucleic acid sequence in the table below and/or:

Transcript Name Z25299_PEA_2_T1 (SEQ ID NO: 256) Z25299_PEA_2_T2 (SEQ ID NO: 257) Z25299_PEA_2_T3 (SEQ ID NO: 258) Z25299_PEA_2_T6 (SEQ ID NO: 259) Z25299_PEA_2_T9 (SEQ ID NO: 260) a nucleic acid sequence comprising a sequence in the table below:

Segment Name Z25299_PEA_2_node_20 (SEQ ID NO: 261) Z25299_PEA_2_node_21 (SEQ ID NO: 262) Z25299_PEA_2_node_23 (SEQ ID NO: 263) Z25299_PEA_2_node_24 (SEQ ID NO: 264) Z25299_PEA_2_node_8 (SEQ ID NO: 265) Z25299_PEA_2_node_12 (SEQ ID NO: 266) Z25299_PEA_2_node_13 (SEQ ID NO: 267) Z25299_PEA_2_node_14 (SEQ ID NO: 268) Z25299_PEA_2_node_17 (SEQ ID NO: 269) Z25299_PEA_2_node_18 (SEQ ID NO: 270) Z25299_PEA_2_node_19 (SEQ ID NO: 271)

According to preferred embodiments of the present invention, there is provided an isolated polypeptide comprising an amino acid sequence in the table below:

Protein Name Z25299_PEA_2_P2 (SEQ ID NO: 273) Z25299_PEA_2_P3 (SEQ ID NO: 274) Z25299_PEA_2_P7 (SEQ ID NO: 275) Z25299_PEA_2_P10 (SEQ ID NO: 276)

According to preferred embodiments of the present invention, there is provided an isolated polynucleotide comprising a nucleic acid sequence in the table below and/or:

Transcript Name T39971_T10 (SEQ ID NO: 570) T39971_T12 (SEQ ID NO: 571) T39971_T16 (SEQ ID NO: 572) T39971_T5 (SEQ ID NO: 573) a nucleic acid sequence comprising a sequence in the table below:

Segment Name T39971_node_0 (SEQ ID NO: 574) T39971_node_18 (SEQ ID NO: 575) T39971_node_21 (SEQ ID NO: 576) T39971_node_22 (SEQ ID NO: 577) T39971_node_23 (SEQ ID NO: 578) T39971_node_31 (SEQ ID NO: 579) T39971_node_33 (SEQ ID NO: 580) T39971_node_7 (SEQ ID NO: 581) T39971_node_1 (SEQ ID NO: 582) T39971_node_10 (SEQ ID NO: 583) T39971_node_11 (SEQ ID NO: 584) T39971_node_12 (SEQ ID NO: 585) T39971_node_15 (SEQ ID NO: 586) T39971_node_16 (SEQ ID NO: 587) T39971_node_17 (SEQ ID NO: 588) T39971_node_26 (SEQ ID NO: 589) T39971_node_27 (SEQ ID NO: 590) T39971_node_28 (SEQ ID NO: 591) T39971_node_29 (SEQ ID NO: 592) T39971_node_3 (SEQ ID NO: 593) T39971_node_30 (SEQ ID NO: 594) T39971_node_34 (SEQ ID NO: 595) T39971_node_35 (SEQ ID NO: 596) T39971_node_36 (SEQ ID NO: 597) T39971_node_4 (SEQ ID NO: 598) T39971_node_5 (SEQ ID NO: 599) T39971_node_8 (SEQ ID NO: 600) T39971_node_9 (SEQ ID NO: 601)

According to preferred embodiments of the present invention, there is provided an isolated polypeptide comprising an amino acid sequence in the table below:

Protein Name T39971_P6 (SEQ ID NO:603) T39971_P9 (SEQ ID NO:604) T39971_P11 (SEQ ID NO:605) T39971_P12 (SEQ ID NO:606)

According to preferred embodiments of the present invention, there is provided an isolated polynucleotide comprising a nucleic acid sequence in the table below and/or:

Transcript Name Z44808_PEA_1_T11 (SEQ ID NO:607) Z44808_PEA_1_T4 (SEQ ID NO:608) Z44808_PEA_1_T5 (SEQ ID NO:609) Z44808_PEA_1_T8 (SEQ ID NO:610) Z44808_PEA_1_T9 (SEQ ID NO:611) a nucleic acid sequence comprising a sequence in the table below:

Segment Name Z44808_PEA_1_node_0 (SEQ ID NO:612) Z44808_PEA_1_node_16 (SEQ ID NO:613) Z44808_PEA_1_node_2 (SEQ ID NO:614) Z44808_PEA_1_node_24 (SEQ ID NO:615) Z44808_PEA_1_node_32 (SEQ ID NO:616) Z44808_PEA_1_node_33 (SEQ ID NO:617) Z44808_PEA_1_node_36 (SEQ ID NO:618) Z44808_PEA_1_node_37 (SEQ ID NO:619) Z44808_PEA_1_node_41 (SEQ ID NO:620) Z44808_PEA_1_node_11 (SEQ ID NO:621) Z44808_PEA_1_node_13 (SEQ ID NO:622) Z44808_PEA_1_node_18 (SEQ ID NO:623) Z44808_PEA_1_node_22 (SEQ ID NO:624) Z44808_PEA_1_node_26 (SEQ ID NO:625) Z44808_PEA_1_node_30 (SEQ ID NO:626) Z44808_PEA_1_node_34 (SEQ ID NO:627) Z44808_PEA_1_node_35 (SEQ ID NO:628) Z44808_PEA_1_node_39 (SEQ ID NO:629) Z44808_PEA_1_node_4 (SEQ ID NO:630) Z44808_PEA_1_node_6 (SEQ ID NO:631) Z44808_PEA_1_node_8 (SEQ ID NO:632)

According to preferred embodiments of the present invention, there is provided an isolated polypeptide comprising an amino acid sequence in the table below:

Protein Name Z44808_PEA_1_P5 (SEQ ID NO:634) Z44808_PEA_1_P6 (SEQ ID NO:635) Z44808_PEA_1_P7 (SEQ ID NO:636) Z44808_PEA_1_P11 (SEQ ID NO:637)

According to preferred embodiments of the present invention, there is provided an isolated polynucleotide comprising a nucleic acid sequence in the table below and/or:

Transcript Name S67314_PEA_1_T4 (SEQ ID NO:638) S67314_PEA_1_T5 (SEQ ID NO:639) S67314_PEA_1_T6 (SEQ ID NO:640) S67314_PEA_1_T7 (SEQ ID NO:641 a nucleic acid sequence comprising a sequence in the table below:

Segment Name S67314_PEA_1_node_0 (SEQ ID NO:642) S67314_PEA_1_node_11 (SEQ ID NO:643) S67314_PEA_1_node_13 (SEQ ID NO:644) S67314_PEA_1_node_15 (SEQ ID NO:645) S67314_PEA_1_node_17 (SEQ ID NO:646) S67314_PEA_1_node_4 (SEQ ID NO:647) S67314_PEA_1_node_10 (SEQ ID NO:648) S67314_PEA_1_node_3 (SEQ ID NO:649)

According to preferred embodiments of the present invention, there is provided an isolated polypeptide comprising an amino acid sequence in the table below:

Protein Name S67314_PEA_1_P4 (SEQ ID NO:651) S67314_PEA_1_P5 (SEQ ID NO:652) S67314_PEA_1_P6 (SEQ ID NO:653) S67314_PEA_1_P7 (SEQ ID NO:654)

According to preferred embodiments of the present invention, there is provided an isolated polynucleotide comprising a nucleic acid sequence in the table below and/or:

Transcript Name Z39337_PEA_2_PEA_1_T3 (SEQ ID NO:655) Z39337_PEA_2_PEA_1_T6 (SEQ ID NO:656) Z39337_PEA_2_PEA_1_T12 (SEQ ID NO:657) a nucleic acid sequence comprising a sequence in the table below:

Segment Name Z39337_PEA_2_PEA_1_node_2 (SEQ ID NO:658) Z39337_PEA_2_PEA_1_node_15 (SEQ ID NO:659) Z39337_PEA_2_PEA_1_node_16 (SEQ ID NO:660) Z39337_PEA_2_PEA_1_node_18 (SEQ ID NO:661) Z39337_PEA_2_PEA_1_node_21 (SEQ ID NO:662) Z39337_PEA_2_PEA_1_node_22 (SEQ ID NO:663) Z39337_PEA_2_PEA_1_node_3 (SEQ ID NO:664) Z39337_PEA_2_PEA_1_node_5 (SEQ ID NO:665) Z39337_PEA_2_PEA_1_node_6 (SEQ ID NO:666) Z39337_PEA_2_PEA_1_node_10 (SEQ ID NO:667) Z39337_PEA_2_PEA_1_node_11 (SEQ ID NO:668) Z39337_PEA_2_PEA_1_node_14 (SEQ ID NO:669)

According to preferred embodiments of the present invention, there is provided an isolated polypeptide comprising an amino acid sequence in the table below:

Protein Name Z39337_PEA_2_PEA_1_P4 (SEQ ID NO:671) Z39337_PEA_2_PEA_1_P9 (SEQ ID NO:672) Z39337_PEA_2_PEA_1_P13 (SEQ ID NO:673)

According to preferred embodiments of the present invention, there is provided an isolated polynucleotide comprising a nucleic acid sequence in the table below and/or:

Transcript Name HUMPHOSLIP_PEA_2_T6 (SEQ ID NO:674) HUMPHOSLIP_PEA_2_T7 (SEQ ID NO:675) HUMPHOSLIP_PEA_2_T14 (SEQ ID NO:676) HUMPHOSLIP_PEA_2_T16 (SEQ ID NO:677) HUMPHOSLIP_PEA_2_T17 (SEQ ID NO:678) HUMPHOSLIP_PEA_2_T18 (SEQ ID NO:679) HUMPHOSLIP_PEA_2_T19 (SEQ ID NO:680) a nucleic acid sequence comprising a sequence in the table below:

Segment Name HUMPHOSLIP_PEA_2_node_0 (SEQ ID NO:681) HUMPHOSLIP_PEA_2_node_19 (SEQ ID NO:682) HUMPHOSLIP_PEA_2_node_34 (SEQ ID NO:683) HUMPHOSLIP_PEA_2_node_68 (SEQ ID NO:684) HUMPHOSLIP_PEA_2_node_70 (SEQ ID NO:685) HUMPHOSLIP_PEA_2_node_75 (SEQ ID NO:686) HUMPHOSLIP_PEA_2_node_2 (SEQ ID NO:687) HUMPHOSLIP_PEA_2_node_3 (SEQ ID NO:688) HUMPHOSLIP_PEA_2_node_4 (SEQ ID NO:689) HUMPHOSLIP_PEA_2_node_6 (SEQ ID NO:690) HUMPHOSLIP_PEA_2_node_7 (SEQ ID NO:691) HUMPHOSLIP_PEA_2_node_8 (SEQ ID NO:692) HUMPHOSLIP_PEA_2_node_9 (SEQ ID NO:693) HUMPHOSLIP_PEA_2_node_14 (SEQ ID NO:694) HUMPHOSLIP_PEA_2_node_15 (SEQ ID NO:695) HUMPHOSLIP_PEA_2_node_16 (SEQ ID NO:696) HUMPHOSLIP_PEA_2_node_17 (SEQ ID NO:697) HUMPHOSLIP_PEA_2_node_23 (SEQ ID NO:698) HUMPHOSLIP_PEA_2_node_24 (SEQ ID NO:699) HUMPHOSLIP_PEA_2_node_25 (SEQ ID NO:700) HUMPHOSLIP_PEA_2_node_26 (SEQ ID NO:701) HUMPHOSLIP_PEA_2_node_29 (SEQ ID NO:702) HUMPHOSLIP_PEA_2_node_30 (SEQ ID NO:703) HUMPHOSLIP_PEA_2_node_33 (SEQ ID NO:704) HUMPHOSLIP_PEA_2_node_36 (SEQ ID NO:705) HUMPHOSLIP_PEA_2_node_37 (SEQ ID NO:706) HUMPHOSLIP_PEA_2_node_39 (SEQ ID NO:707) HUMPHOSLIP_PEA_2_node_40 (SEQ ID NO:708) HUMPHOSLIP_PEA_2_node_41 (SEQ ID NO:709) HUMPHOSLIP_PEA_2_node_42 (SEQ ID NO:710) HUMPHOSLIP_PEA_2_node_44 (SEQ ID NO:711) HUMPHOSLIP_PEA_2_node_45 (SEQ ID NO:712) HUMPHOSLIP_PEA_2_node_47 (SEQ ID NO:713) HUMPHOSLIP_PEA_2_node_51 (SEQ ID NO:714) HUMPHOSLIP_PEA_2_node_52 (SEQ ID NO:715) HUMPHOSLIP_PEA_2_node_53 (SEQ ID NO:716) HUMPHOSLIP_PEA_2_node_54 (SEQ ID NO:717) HUMPHOSLIP_PEA_2_node_55 (SEQ ID NO:718) HUMPHOSLIP_PEA_2_node_58 (SEQ ID NO:719) HUMPHOSLIP_PEA_2_node_59 (SEQ ID NO:720) HUMPHOSLIP_PEA_2_node_60 (SEQ ID NO:721) HUMPHOSLIP_PEA_2_node_61 (SEQ ID NO:722) HUMPHOSLIP_PEA_2_node_62 (SEQ ID NO:723) HUMPHOSLIP_PEA_2_node_63 (SEQ ID NO:724) HUMPHOSLIP_PEA_2_node_64 (SEQ ID NO:725) HUMPHOSLIP_PEA_2_node_65 (SEQ ID NO:726) HUMPHOSLIP_PEA_2_node_66 (SEQ ID NO:727) HUMPHOSLIP_PEA_2_node_67 (SEQ ID NO:728) HUMPHOSLIP_PEA_2_node_69 (SEQ ID NO:729) HUMPHOSLIP_PEA_2_node_71 (SEQ ID NO:730) HUMPHOSLIP_PEA_2_node_72 (SEQ ID NO:731) HUMPHOSLIP_PEA_2_node_73 (SEQ ID NO.732) HUMPHOSLIP_PEA_2_node_74 (SEQ ID NO:733)

According to preferred embodiments of the present invention, there is provided an isolated polypeptide comprising an amino acid sequence in the table below:

Protein Name HUMPHOSLIP_PEA_2_P10 (SEQ ID NO:735) HUMPHOSLIP_PEA_2_P12 (SEQ ID NO:736) HUMPHOSLIP_PEA_2_P30 (SEQ ID NO:737) HUMPHOSLIP_PEA_2_P31 (SEQ ID NO:738) HUMPHOSLIP_PEA_2_P33 (SEQ ID NO:739) HUMPHOSLIP_PEA_2_P34 (SEQ ID NO:740) HUMPHOSLIP_PEA_2_P35 (SEQ ID NO:741)

According to preferred embodiments of the present invention, there is provided an isolated polynucleotide comprising a nucleic acid sequence in the table below and/or:

Transcript Name T59832_T6 (SEQ ID NO:742) T59832_T8 (SEQ ID NO:743) T59832_T11 (SEQ ID NO:744) T59832_T15 (SEQ ID NO:745) T59832_T22 (SEQ ID NO:746) a nucleic acid sequence comprising a sequence in the table below:

Segment Name T59832_node_1 (SEQ ID NO:747) T59832_node_7 (SEQ ID NO:748) T59832_node_29 (SEQ ID NO:749) T59832_node_39 (SEQ ID NO:750) T59832_node_2 (SEQ ID NO:751) T59832_node_3 (SEQ ID NO:752) T59832_node_4 (SEQ ID NO:753) T59832_node_5 (SEQ ID NO:754) T59832_node_6 (SEQ ID NO:755) T59832_node_8 (SEQ ID NO:756) T59832_node_9 (SEQ ID NO:757) T59832_node_10 (SEQ ID NO:758) T59832_node_11 (SEQ ID NO:759) T59832_node_12 (SEQ ID NO:760) T59832_node_14 (SEQ ID NO:761) T59832_node_16 (SEQ ID NO:762) T59832_node_19 (SEQ ID NO:763) T59832_node_20 (SEQ ID NO:764) T59832_node_25 (SEQ ID NO:765) T59832_node_26 (SEQ ID NO:766) T59832_node_27 (SEQ ID NO:767) T59832_node_28 (SEQ ID NO:768) T59832_node_30 (SEQ ID NO:769) T59832_node_31 (SEQ ID NO:770) T59832_node_32 (SEQ ID NO:771) T59832_node_34 (SEQ ID NO:772) T59832_node_35 (SEQ ID NO:773) T59832_node_36 (SEQ ID NO:774) T59832_node_37 (SEQ ID NO:775) T59832_node_38 (SEQ ID NO:776)

According to preferred embodiments of the present invention, there is provided an isolated polypeptide comprising an amino acid sequence in the table below:

Protein Name T59832_P5 (SEQ ID NO:778) T59832_P7 (SEQ ID NO:779) T59832_P9 (SEQ ID NO:780) T59832_P12 (SEQ ID NO:781) T59832_P18 (SEQ ID NO:782)

According to preferred embodiments of the present invention, there is provided an isolated polynucleotide comprising a nucleic acid sequence in the table below and/or:

Transcript Name HSCP2_PEA_1_T4 (SEQ ID NO:783) HSCP2_PEA_1_T13 (SEQ ID NO:784) HSCP2_PEA_1_T19 (SEQ ID NO:785) HSCP2_PEA_1_T20 (SEQ ID NO:786) HSCP2_PEA_1_T22 (SEQ ID NO:787) HSCP2_PEA_1_T23 (SEQ ID NO:788) HSCP2_PEA_1_T25 (SEQ ID NO:789) HSCP2_PEA_1_T31 (SEQ ID NO:790) HSCP2_PEA_1_T33 (SEQ ID NO:791) HSCP2_PEA_1_T34 (SEQ ID NO:792) HSCP2_PEA_1_T45 (SEQ ID NO:793) HSCP2_PEA_1_T50 (SEQ ID NO:794) a nucleic acid sequence comprising a sequence in the table below:

Segment Name HSCP2_PEA_1_node_0 (SEQ ID NO:795) HSCP2_PEA_1_node_3 (SEQ ID NO:796) HSCP2_PEA_1_node_6 (SEQ ID NO:797) HSCP2_PEA_1_node_8 (SEQ ID NO:798) HSCP2_PEA_1_node_10 (SEQ ID NO:799) HSCP2_PEA_1_node_14 (SEQ ID NO:800) HSCP2_PEA_1_node_23 (SEQ ID NO:801) HSCP2_PEA_1_node_26 (SEQ ID NO:802) HSCP2_PEA_1_node_29 (SEQ ID NO:803) HSCP2_PEA_1_node_31 (SEQ ID NO:804) HSCP2_PEA_1_node_32 (SEQ ID NO:805) HSCP2_PEA_1_node_34 (SEQ ID NO:806) HSCP2_PEA_1_node_52 (SEQ ID NO:807) HSCP2_PEA_1_node_58 (SEQ ID NO:808) HSCP2_PEA_1_node_72 (SEQ ID NO:809) HSCP2_PEA_1_node_73 (SEQ ID NO:810) HSCP2_PEA_1_node_74 (SEQ ID NO:811) HSCP2_PEA_1_node_76 (SEQ ID NO:812) HSCP2_PEA_1_node_78 (SEQ ID NO:813) HSCP2_PEA_1_node_80 (SEQ ID NO:814) HSCP2_PEA_1_node_84 (SEQ ID NO:815) HSCP2_PEA_1_node_4 (SEQ ID NO. 816) HSCP2_PEA_1_node_7 (SEQ ID NO:817) HSCP2_PEA_1_node_13 (SEQ ID NO:818) HSCP2_PEA_1_node_15 (SEQ ID NO:819) HSCP2_PEA_1_node_16 (SEQ ID NO:820) HSCP2_PEA_1_node_18 (SEQ ID NO:821) HSCP2_PEA_1_node_20 (SEQ ID NO:822) HSCP2_PEA_1_node_21 (SEQ ID NO:823) HSCP2_PEA_1_node_37 (SEQ ID NO:824) HSCP2_PEA_1_node_38 (SEQ ID NO:825) HSCP2_PEA_1_node_39 (SEQ TD NO:826) HSCP2_PEA_1_node_41 (SEQ ID NO:827) HSCP2_PEA_1_node_42 (SEQ ID NO:828) HSCP2_PEA_1_node_46 (SEQ ID NO:829) HSCP2_PEA_1_node_47 (SEQ ID NO:830) HSCP2_PEA_1_node_50 (SEQ ID NO:831) HSCP2_PEA_1_node_51 (SEQ ID NO:832) HSCP2_PEA_1_node_55 (SEQ ID NO:833) HSCP2_PEA_1_node_56 (SEQ ID NO:834) HSCP2_PEA_1_node_60 (SEQ ID NO:835) HSCP2_PEA_1_node_61 (SEQ ID NO:836) HSCP2_PEA_1_node_67 (SEQ ID NO:837) HSCP2_PEA_1_node_68 (SEQ ID NO:838) HSCP2_PEA_1_node_69 (SEQ ID NO:839) HSCP2_PEA_1_node_70 (SEQ ID NO:840) HSCP2_PEA_1_node_75 (SEQ ID NO:841) HSCP2_PEA_1_node_77 (SEQ ID NO:842) HSCP2_PEA_1_node_79 (SEQ ID NO:843) HSCP2_PEA_1_node_82 (SEQ ID NO:844)

According to preferred embodiments of the present invention, there is provided an isolated polypeptide comprising an amino acid sequence in the table below:

Protein Name HSCP2_PEA_1_P4 (SEQ ID NO:846) HSCP2_PEA_1_P8 (SEQ ID NO:847) HSCP2_PEA_1_P14 (SEQ ID NO:848) HSCP2_PEA_1_P15 (SEQ ID NO:849) HSCP2_PEA_1_P2 (SEQ ID NO:850) HSCP2_PEA_1_P16 (SEQ ID NO:851) HSCP2_PEA_1_P6 (SEQ ID NO:852) HSCP2_PEA_1_P22 (SEQ ID NO:853) HSCP2_PEA_1_P24 (SEQ ID NO:854) HSCP2_PEA_1_P25 (SEQ ID NO:855) HSCP2_PEA_1_P33 (SEQ ID NO:856)

According to preferred embodiments of the present invention, there is provided an isolated polynucleotide comprising a nucleic acid sequence in the table below and/or:

Transcript Name HUMTEN_PEA_1_T4 (SEQ ID NO:857) HUMTEN_PEA_1_T5 (SEQ ID NO:858) HUMTEN_PEA_1_T6 (SEQ ID NO:859) HUMTEN_PEA_1_T7 (SEQ ID NO:860) HUMTEN_PEA_1_T11 (SEQ ID NO:861) HUMTEN_PEA_1_T14 (SEQ ID NO:862) HUMTEN_PEA_1_T16 (SEQ ID NO:863) HUMTEN_PEA_1_T17 (SEQ ID NO:864) HUMTEN_PEA_1_T18 (SEQ ID NO:865) HUMTEN_PEA_1_T19 (SEQ ID NO:866) HUMTEN_PEA_1_T20 (SEQ ID NO:867) HUMTEN_PEA_1_T23 (SEQ ID NO.868) HUMTEN_PEA_1_T32 (SEQ ID NO:869) HUMTEN_PEA_1_T35 (SEQ ID NO:870) HUMTEN_PEA_1_T36 (SEQ ID NO:871) HUMTEN_PEA_1_T37 (SEQ ID NO:872) HUMTEN_PEA_1_T39 (SEQ ID NO:873) HUMTEN_PEA_1_T40 (SEQ ID NO:874) HUMTEN_PEA_1_T41 (SEQ ID NO:875) a nucleic acid sequence comprising a sequence in the table below:

Segment Name HUMTEN_PEA_1_node_0 (SEQ ID NO:876) HUMTEN_PEA_1_node_2 (SEQ ID NO:877) HUMTEN_PEA_1_node_5 (SEQ ID NO:878) HUMTEN_PEA_1_node_6 (SEQ ID NO:879) HUMTEN_PEA_1_node_11 (SEQ ID NO:880) HUMTEN_PEA_1_node_12 (SEQ ID NO:881) HUMTEN_PEA_1_node_16 (SEQ ID NO:882) HUMTEN_PEA_1_node_19 (SEQ ID NO:883) HUMTEN_PEA_1_node_23 (SEQ ID NO:884) HUMTEN_PEA_1_node_27 (SEQ ID NO:885) HUMTEN_PEA_1_node_28 (SEQ ID NO:886) HUMTEN_PEA_1_node_30 (SEQ ID NO:887) HUMTEN_PEA_1_node_32 (SEQ ID NO:888) HUMTEN_PEA_1_node_33 (SEQ ID NO:889) HUMTEN_PEA_1_node_35 (SEQ ID NO:890) HUMTEN_PEA_1_node_38 (SEQ ID NO:891) HUMTEN_PEA_1_node_40 (SEQ ID NO:892) HUMTEN_PEA_1_node_42 (SEQ ID NO:893) HUMTEN_PEA_1_node_43 (SEQ ID NO:894) HUMTEN_PEA_1_node_44 (SEQ ID NO:895) HUMTEN_PEA_1_node_45 (SEQ ID NO:896) HUMTEN_PEA_1_node_46 (SEQ ID NO:897) HUMTEN_PEA_1_node_47 (SEQ ID NO:898) HUMTEN_PEA_1_node_49 (SEQ ID NO:899) HUMTEN_PEA_1_node_51 (SEQ ID NO:900) HUMTEN_PEA_1_node_56 (SEQ ID NO:901) HUMTEN_PEA_1_node_65 (SEQ ID NO:902) HUMTEN_PEA_1_node_71 (SEQ ID NO:903) HUMTEN_PEA_1_node_73 (SEQ ID NO:904) HUMTEN_PEA_1_node_76 (SEQ ID NO:905) HUMTEN_PEA_1_node_79 (SEQ ID NO:906) HUMTEN_PEA_1_node_83 (SEQ ID NO:907) HUMTEN_PEA_1_node_89 (SEQ ID NO:908) HUMTEN_PEA_1_node_7 (SEQ ID NO:909) HUMTEN_PEA_1_node_8 (SEQ ID NO:910) HUMTEN_PEA_1_node_9 (SEQ ID NO:911) HUMTEN_PEA_1_node_14 (SEQ ID NO:912) HUMTEN_PEA_1_node_17 (SEQ ID NO.913) HUMTEN_PEA_1_node_21 (SEQ ID NO:914) HUMTEN_PEA_1_node_22 (SEQ ID NO:915) HUMTEN_PEA_1_node_25 (SEQ ID NO:916) HUMTEN_PEA_1_node_36 (SEQ ID NO:917) HUMTEN_PEA_1_node_53 (SEQ ID NO:918) HUMTEN_PEA_1_node_54 (SEQ ID NO:919)) HUMTEN_PEA_1_node_57 (SEQ ID NO:920) HUMTEN_PEA_1_node_61 (SEQ ID NO:921) HUMTEN_PEA_1_node_62 (SEQ ID NO:922) HUMTEN_PEA_1_node_67 (SEQ ID NO:923) HUMTEN_PEA_1_node_68 (SEQ ID NO:924) HUMTEN_PEA_1_node_69 (SEQ ID NO:925) HUMTEN_PEA_1_node_70 (SEQ ID NO:926) HUMTEN_PEA_1_node_72 (SEQ ID NO:927) HUMTEN_PEA_1_node_84 (SEQ ID NO:928) HUMTEN_PEA_1_node_85 (SEQ ID NO:929) HUMTEN_PEA_1_node_86 (SEQ ID NO:930) HUMTEN_PEA_1_node_87 (SEQ ID NO:931) HUMTEN_PEA_1_node_88 (SEQ ID NO:932)

According to preferred embodiments of the present invention, there is provided an isolated polypeptide comprising an amino acid sequence in the table below:

Protein Name HUMTEN_PEA_1_P5 (SEQ ID NO:934) HUMTEN_PEA_1_P6 (SEQ ID NO:935) HUMTEN_PEA_1_P7 (SEQ ID NO:936) HUMTEN_PEA_1_P8 (SEQ ID NO:937) HUMTEN_PEA_1_P10 (SEQ ID NO:938) HUMTEN_PEA_1_P11 (SEQ ID NO:939) HUMTEN_PEA_1_P13 (SEQ ID NO:940) HUMTEN_PEA_1_P14 (SEQ ID NO:941) HUMTEN_PEA_1_P15 (SEQ ID NO:942) HUMTEN_PEA_1_P16 (SEQ ID NO:943) HUMTEN_PEA_1_P17 (SEQ ID NO:944) HUMTEN_PEA_1_P20 (SEQ ID NO:945) HUMTEN_PEA_1_P26 (SEQ ID NO:946) HUMTEN_PEA_1_P27 (SEQ ID NO:947) HUMTEN_PEA_1_P28 (SEQ ID NO:948) HUMTEN_PEA_1_P29 (SEQ ID NO:949) HUMTEN_PEA_1_P30 (SEQ ID NO:950) HUMTEN_PEA_1_P31 (SEQ ID NO:951) HUMTEN_PEA_1_P32 (SEQ ID NO:952)

According to preferred embodiments of the present invention, there is provided an isolated polynucleotide comprising a nucleic acid sequence in the table below and/or:

Transcript Name HUMOSTRO_PEA_1_PEA_1_T14 (SEQ ID NO:277) HUMOSTRO_PEA_1_PEA_1_T16 (SEQ ID NO:278) HUMOSTRO_PEA_1_PEA_1_T30 (SEQ ID NO:279) a nucleic acid sequence comprising a sequence in the table below:

Segment Name HUMOSTRO_PEA_1_PEA_1_node_0 (SEQ ID NO:280) HUMOSTRO_PEA_1_PEA_1_node_10 (SEQ ID NO:281) HUMOSTRO_PEA_1_PEA_1_node_16 (SEQ ID NO:282) HUMOSTRO_PEA_1_PEA_1_node_23 (SEQ ID NO:283) HUMOSTRO_PEA_1_PEA_1_node_31 (SEQ ID NO:284) HUMOSTRO_PEA_1_PEA_1_node_43 (SEQ ID NO:285) HUMOSTRO_PEA_1_PEA_1_node_3 (SEQ ID NO:286) HUMOSTRO_PEA_1_PEA_1_node_5 (SEQ ID NO:287) HUMOSTRO_PEA_1_PEA_1_node_7 (SEQ ID NO:288) HUMOSTRO_PEA_1_PEA_1_node_8 (SEQ ID NO:289) HUMOSTRO_PEA_1_PEA_1_node_15 (SEQ ID NO:290) HUMOSTRO_PEA_1_PEA_1_node_17 (SEQ ID NO:291) HUMOSTRO_PEA_1_PEA_1_node_20 (SEQ ID NO:292) HUMOSTRO_PEA_1_PEA_1_node_21 (SEQ ID NO:293) HUMOSTRO_PEA_1_PEA_1_node_22 (SEQ ID NO:294) HUMOSTRO_PEA_1_PEA_1_node_24 (SEQ ID NO:295) HUMOSTRO_PEA_1_PEA_1_node_26 (SEQ ID NO:296) HUMOSTRO_PEA_1_PEA_1_node_27 (SEQ ID NO:297) HUMOSTRO_PEA_1_PEA_1_node_28 (SEQ ID NO:298) HUMOSTRO_PEA_1_PEA_1_node_29 (SEQ ID NO:299) HUMOSTRO_PEA_1_PEA_1_node_30 (SEQ ID NO:300) HUMOSTRO_PEA_1_PEA_1_node_32 (SEQ ID NO:301) HUMOSTRO_PEA_1_PEA_1_node_34 (SEQ ID NO:302) HUMOSTRO_PEA_1_PEA_1_node_36 (SEQ ID NO:303) HUMOSTRO_PEA_1_PEA_1_node_37 (SEQ ID NO:304) HUMOSTRO_PEA_1_PEA_1_node_38 (SEQ ID NO:305) HUMOSTRO_PEA_1_PEA_1_node_39 (SEQ ID NO:306) HUMOSTRO_PEA_1_PEA_1_node_40 (SEQ ID NO:307) HUMOSTRO_PEA_1_PEA_1_node_41 (SEQ ID NO:308) HUMOSTRO_PEA_1_PEA_1_node_42 (SEQ ID NO:309)

According to preferred embodiments of the present invention, there is provided an isolated polypeptide comprising an amino acid sequence in the table below:

Protein Name HUMOSTRO_PEA_1_PEA_1_P21 (SEQ ID NO:311) HUMOSTRO_PEA_1_PEA_1_P25 (SEQ ID NO:312) HUMOSTRO_PEA_1_PEA_1_P30 (SEQ ID NO:313)

According to preferred embodiments of the present invention, there is provided an isolated polynucleotide comprising a nucleic acid sequence in the table below and/or:

Transcript Name T46984_PEA_1_T2 (SEQ ID NO:314) T46984_PEA_1_T3 (SEQ ID NO:315) T46984_PEA_1_T12 (SEQ ID NO:316) T46984_PEA_1_T13 (SEQ ID NO:317) T46984_PEA_1_T14 (SEQ ID NO:318) T46984_PEA_1_T15 (SEQ ID NO:319) T46984_PEA_1_T19 (SEQ ID NO:320) T46984_PEA_1_T23 (SEQ ID NO:321) T46984_PEA_1_T27 (SEQ ID NO:322) T46984_PEA_1_T32 (SEQ ID NO:323) T46984_PEA_1_T34 (SEQ ID NO:324) T46984_PEA_1_T35 (SEQ ID NO:325) T46984_PEA_1_T40 (SEQ ID NO:326) T46984_PEA_1_T42 (SEQ ID NO:327) T46984_PEA_1_T43 (SEQ ID NO:328) T46984_PEA_1_T46 (SEQ ID NO:329) T46984_PEA_1_T47 (SEQ ID NO:330) T46984_PEA_1_T48 (SEQ ID NO:331) T46984_PEA_1_T51 (SEQ ID NO:332) T46984_PEA_1_T52 (SEQ ID NO:333) T46984_PEA_1_T54 (SEQ ID NO:334) a nucleic acid sequence comprising a sequence in the table below:

Segment Name T46984_PEA_1_node_2 (SEQ ID NO:335) T46984_PEA_1_node_4 (SEQ ID NO:336) T46984_PEA_1_node_6 (SEQ ID NO:337) T46984_PEA_1_node_12 (SEQ ID NO:338) T46984_PEA_1_node_14 (SEQ ID NO:339) T46984_PEA_1_node_25 (SEQ ID NO:340) T46984_PEA_1_node_29 (SEQ ID NO:341) T46984_PEA_1_node_34 (SEQ ID NO:342) T46984_PEA_1_node_46 (SEQ ID NO:343) T46984_PEA_1_node_47 (SEQ ID NO:344) T46984_PEA_1_node_52 (SEQ ID NO:345) T46984_PEA_1_node_65 (SEQ ID NO:346) T46984_PEA_1_node_69 (SEQ ID NO:347) T46984_PEA_1_node_75 (SEQ ID NO:348) T46984_PEA_1_node_86 (SEQ ID NO:349) T46984_PEA_1_node_9 (SEQ ID NO:350) T46984_PEA_1_node_13 (SEQ ID NO:351) T46984_PEA_1_node_19 (SEQ ID NO:352) T46984_PEA_1_node_21 (SEQ ID NO:353) T46984_PEA_1_node_22 (SEQ ID NO:354) T46984_PEA_1_node_26 (SEQ ID NO:355) T46984_PEA_1_node_28 (SEQ ID NO.356) T46984_PEA_1_node_31 (SEQ ID NO:357) T46984_PEA_1_node_32 (SEQ ID NO:358) T46984_PEA_1_node_38 (SEQ ID NO:359) T46984_PEA_1_node_39 (SEQ ID NO:360) T46984_PEA_1_node_40 (SEQ ID NO:361) T46984_PEA_1_node_42 (SEQ ID NO:362) T46984_PEA_1_node_43 (SEQ ID NO:363) T46984_PEA_1_node_48 (SEQ ID NO:364) T46984_PEA_1_node_49 (SEQ ID NO:365) T46984_PEA_1_node_50 (SEQ ID NO:366) T46984_PEA_1_node_51 (SEQ ID NO:367) T46984_PEA_1_node_53 (SEQ ID NO:368) T46984_PEA_1_node_54 (SEQ ID NO:369) T46984_PEA_1_node_55 (SEQ ID NO:370) T46984_PEA_1_node_57 (SEQ ID NO:371) T46984_PEA_1_node_60 (SEQ ID NO:372) T46984_PEA_1_node_62 (SEQ ID NO:373) T46984_PEA_1_node_66 (SEQ ID NO:374) T46984_PEA_1_node_67 (SEQ ID NO:375) T46984_PEA_1_node_70 (SEQ ID NO:376) T46984_PEA_1_node_71 (SEQ ID NO:377) T46984_PEA_1_node_72 (SEQ ID NO.378) T46984_PEA_1_node_73 (SEQ ID NO:379) T46984_PEA_1_node_74 (SEQ ID NO:380) T46984_PEA_1_node_83 (SEQ ID NO:381) T46984_PEA_1_node_84 (SEQ ID NO:382) T46984_PEA_1_node_85 (SEQ ID NO:383)

According to preferred embodiments of the present invention, there is provided an isolated polypeptide comprising an amino acid sequence in the table below:

Protein Name T46984_PEA_1_P2 (SEQ ID NO:385) T46984_PEA_1_P3 (SEQ ID NO:386) T46984_PEA_1_P10 (SEQ ID NO:387) T46984_PEA_1_P11 (SEQ ID NO:388) T46984_PEA_1_P12 (SEQ ID NO:389) T46984_PEA_1_P21 (SEQ ID NO:390) T46984_PEA_1_P27 (SEQ ID NO:391) T46984_PEA_1_P32 (SEQ ID NO:392) T46984_PEA_1_P34 (SEQ ID NO:393) T46984_PEA_1_P35 (SEQ ID NO:394) T46984_PEA_1_P38 (SEQ ID NO:395) T46984_PEA_1_P39 (SEQ ID NO.396) T46984_PEA_1_P45 (SEQ ID NO:397) T46984_PEA_1_P46 (SEQ ID NO:398)

According to preferred embodiments of the present invention, there is provided an isolated polynucleotide comprising a nucleic acid sequence in the table below and/or:

Transcript Name M78530_PEA_1_T11 (SEQ ID NO:399) M78530_PEA_1_T12 (SEQ ID NO:400) M78530_PEA_1_T13 (SEQ ID NO:401) a nucleic acid sequence comprising a sequence in the table below:

Segment Name M78530_PEA_1_node_0 (SEQ ID NO:402) M78530_PEA_1_node_15 (SEQ ID NO:403) M78530_PEA_1_node_16 (SEQ ID NO:404) M78530_PEA_1_node_19 (SEQ ID NO:405) M78530_PEA_1_node_21 (SEQ ID NO:406) M78530_PEA_1_node_23 (SEQ ID NO:407) M78530_PEA_1_node_27 (SEQ ID NO:408) M78530_PEA_1_node_29 (SEQ ID NO:409) M78530_PEA_1_node_36 (SEQ ID NO:410) M78530_PEA_1_node_37 (SEQ ID NO:411) M78530_PEA_1_node_2 (SEQ ID NO:412) M78530_PEA_1_node_4 (SEQ ID NO:413) M78530_PEA_1_node_5 (SEQ ID NO:414) M78530_PEA_1_node_7 (SEQ ID NO:415) M78530_PEA_1_node_9 (SEQ ID NO:416) M78530_PEA_1_node_10 (SEQ ID NO:417) M78530_PEA_1_node_18 (SEQ ID NO:418) M78530_PEA_1_node_25 (SEQ ID NO:419) M78530_PEA_1_node_30 (SEQ ID NO:420) M78530_PEA_1_node_33 (SEQ ID NO:421) M78530_PEA_1_node_34 (SEQ ID NO:422)

According to preferred embodiments of the present invention, there is provided an isolated polypeptide comprising an amino acid sequence in the table below:

Protein Name M78530_PEA_1_P15 (SEQ ID NO:426) M78530_PEA_1_P16 (SEQ ID NO:427) M78530_PEA_1_P17 (SEQ ID NO:428)

According to preferred embodiments of the present invention, there is provided an isolated polynucleotide comprising a nucleic acid sequence in the table below and/or:

Transcript Name T48119_T2 (SEQ ID NO:429) a nucleic acid sequence comprising a sequence in the table below:

Segment Name T48119_node_0 (SEQ ID NO:430) T48119_node_11 (SEQ ID NO:431) T48119_node_13 (SEQ ID NO:432) T48119_node_38 (SEQ ID NO:433) T48119_node_41 (SEQ ID NO:434) T48119_node_45 (SEQ ID NO:435) T48119_node_47 (SEQ ID NO:436) T48119_node_4 (SEQ ID NO:437) T48119_node_8 (SEQ ID NO:438) T48119_node_15 (SEQ ID NO:439) T48119_node_17 (SEQ ID NO:440) T48119_node_20 (SEQ ID NO:441) T48119_node_22 (SEQ ID NO:442) T48119_node_26 (SEQ ID NO:443) T48119_node_28 (SEQ ID NO:444) T48119_node_31 (SEQ ID NO:445) T48119_node_32 (SEQ ID NO:446) T48119_node_33 (SEQ ID NO:447) T48119_node_44 (SEQ ID NO:448)

According to preferred embodiments of the present invention, there is provided an isolated polypeptide comprising an amino acid sequence in the table below:

Protein Name T48119_P2 (SEQ ID NO:450)

According to preferred embodiments of the present invention, there is provided an isolated polynucleotide comprising a nucleic acid sequence in the table below and/or:

Transcript Name HSMUC1A_PEA_1_T12 (SEQ ID NO:451) HSMUC1A_PEA_1_T26 (SEQ ID NO:452) HSMUC1A_PEA_1_T28 (SEQ ID NO:453) HSMUC1A_PEA_1_T29 (SEQ ID NO:454) HSMUC1A_PEA_1_T30 (SEQ ID NO:455) HSMUC1A_PEA_1_T31 (SEQ ID NO:456) HSMUC1A_PEA_1_T33 (SEQ ID NO:457) HSMUC1A_PEA_1_T34 (SEQ ID NO:458) HSMUC1A_PEA_1_T35 (SEQ ID NO:459) HSMUC1A_PEA_1_T36 (SEQ ID NO:460) HSMUC1A PEA_1_T40 (SEQ ID NO:461) HSMUC1A_PEA_1_T42 (SEQ ID NO:462) HSMUC1A_PEA_1_T43 (SEQ ID NO:463) HSMUC1A_PEA_1_T47 (SEQ ID NO:464) a nucleic acid sequence comprising a sequence in the table below:

Segment Name HSMUC1A_PEA_1_node_0 (SEQ ID NO:465) HSMUC1A_PEA_1_node_14 (SEQ ID NO:466) HSMUC1A_PEA_1_node_24 (SEQ ID NO:467) HSMUC1A_PEA_1_node_29 (SEQ ID NO:468) HSMUC1A_PEA_1_node_35 (SEQ ID NO:469) HSMUC1A_PEA_1_node_38 (SEQ ID NO:470) HSMUC1A_PEA_1_node_3 (SEQ ID NO:471) HSMUC1A_PEA_1_node_4 (SEQ ID NO:472) HSMUC1A_PEA_1_node_5 (SEQ ID NO:473) HSMUC1A_PEA_1_node_6 (SEQ ID NO:474) HSMUC1A_PEA_1_node_7 (SEQ ID NO:475) HSMUC1A_PEA_1_node_17 (SEQ ID NO:476) HSMUC1A_PEA_1_node_18 (SEQ ID NO:477) HSMUC1A_PEA_1_node_20 (SEQ ID NO:478) HSMUC1A_PEA_1_node_21 (SEQ ID NO:479) HSMUC1A_PEA_1_node_23 (SEQ ID NO:480) HSMUC1A_PEA_1_node_26 (SEQ ID NO:481) HSMUC1A_PEA_1_node_27 (SEQ ID NO:482) HSMUC1A_PEA_1_node_31 (SEQ ID NO:483) HSMUC1A_PEA_1_node_34 (SEQ ID NO:484) HSMUC1A_PEA_1_node_36 (SEQ ID NO:485) HSMUC1A_PEA_1_node_37 (SEQ ID NO:486)

According to preferred embodiments of the present invention, there is provided an isolated polypeptide comprising an amino acid sequence in the table below:

Protein Name HSMUC1A_PEA_1_P25 (SEQ ID NO:488) HSMUC1A_PEA_1_P29 (SEQ ID NO:489) HSMUC1A_PEA_1_P30 (SEQ ID NO:490) HSMUC1A_PEA_1_P32 (SEQ ID NO:491) HSMUC1A_PEA_1_P36 (SEQ ID NO:492) HSMUC1A_PEA_1_P39 (SEQ ID NO:493) HSMUC1A_PEA_1_P45 (SEQ ID NO:494) HSMUC1A_PEA_1_P49 (SEQ ID NO:495) HSMUC1A_PEA_1_P52 (SEQ ID NO:496) HSMUC1A_PEA_1_P53 (SEQ ID NO:497) HSMUC1A_PEA_1_P56 (SEQ ID NO:498) HSMUC1A_PEA_1_P58 (SEQ ID NO:499) HSMUC1A_PEA_1_P59 (SEQ ID NO:500) HSMUC1A_PEA_1_P63 (SEQ ID NO:501)

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for HSMUC1A_PEA_(—)1_P63 (SEQ ID NO: 501), comprising a first amino acid sequence being at least 90% homologous to MTPGTQSPFFLLLLLTVLTVVTGSGHASSTPGGEKETSATQRSSV corresponding to amino acids 1-45 of MUC1_HUMAN, which also corresponds to amino acids 1-45 of HSMUC1A_PEA_(—)1_P63 (SEQ ID NO: 501), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence EEEVSADQVSVGASGVLGSFKEARNAPSFLSWSFSMGPSK (SEQ ID NO: 1060) corresponding to amino acids 46-85 of HSMUC1A_PEA_(—)1_P63 (SEQ ID NO: 501), wherein said first amino acid sequence and second amino acid sequence are contiguous and in a sequential order.

According to preferred embodiments of the present invention, there is provided an isolated polypeptide encoding for a tail of HSMUC1A_PEA_(—)1_P63 (SEQ ID NO: 501), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence EEEVSADQVSVGASGVLGSFKEARNAPSFLSWSFSMGPSK (SEQ ID NO: 1060) in HSMUC1A_PEA_(—)1_P63 (SEQ ID NO: 501).

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for T46984_PEA_(—)1_P2 (SEQ ID NO: 385), comprising a first amino acid sequence being at least 90% homologous to MAPPGSSTVFLLALTIIASTWALTPTHYLTKHDVERLKASLDRPFTNLESAFYSIVGLSSL GAQVPDAKKACTYIRSNLDPSNVDSLFYAAQASQALSGCEISISNETKDLLLAAVSEDSS VTQIYHAVAALSGFGLPLASQEALSALTARLSKEETVLATVQALQTASHLSQQADLRSI VEEIEDLVARLDELGGVYLQFEEGLETTALFVAATYKLMDHVGTEPSIKEDQVIQLMNA IFSKKNFESLSEAFSVASAAAVLSHNRYHVPVVVVPEGSASDTHEQAILRLQVTNVLSQ PLTQATVKLEHAKSVASRATVLQKTSFTPVGDVFELNFMNVKFSSGYYDFLVEVEGDN RYIANTVELRVKISTEVGITNVDLSTVDKDQSIAPKTTRVTYPAKAKGTFIADSHQNFAL FFQLVDVNTGAELTPHQTFVRLHNQKTGQEVVFVAEPDNKNVYKFELDTSERKIEFDS ASGTYTLYLIIGDATLKNPILWNV corresponding to amino acids 1-498 of RIB2_HUMAN, which also corresponds to amino acids 1-498 of T46984_PEA_(—)1_P2 (SEQ ID NO: 385), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence VCA corresponding to amino acids 499-501 of T46984_PEA_(—)1_P2 (SEQ ID NO: 385), wherein said first amino acid sequence and second amino acid sequence are contiguous and in a sequential order.

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for T46984_PEA_(—)1_P3 (SEQ ID NO: 386), comprising a first amino acid sequence being at least 90% homologous to MAPPGSSTVFLLALTIIASTWALTPTHYLTKHDVERLKASLDRPFTNLESAFYSIVGLSSL GAQVPDAKKACTYIRSNLDPSNVDSLFYAAQASQALSGCEISISNETKDLLLAAVSEDSS VTQIYHAVAALSGFGLPLASQEALSALTARLSKEETVLATVQALQTASHLSQQADLRSI VEEIEDLVARLDELGGVYLQFEEGLETTALFVAATYKLMDHVGTEPSIKEDQVIQLMNA IFSKKNFESLSEAFSVASAAAVLSHNRYHVPVVVVPEGSASDTHEQAILRLQVTNVLSQ PLTQATVKLEHAKSVASRATVLQKTSFTPVGDVFELNFMNVKFSSGYYDFLVEVEGDN RYIANTVELRVKISTEVGITNVDLSTVDKDQSIAPKTTRVTYPAKAKGTFIADSHQNFAL FFQLVDVNTGAELTPHQ corresponding to amino acids 1-433 of RIB2_HUMAN, which also corresponds to amino acids 1-433 of T46984_PEA_(—)1_P3 (SEQ ID NO: 386), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence ICHIWKLIFLP (SEQ ID NO: 1061) corresponding to amino acids 434-444 of T46984_PEA_(—)1_P3 (SEQ ID NO: 386), wherein said first amino acid sequence and second amino acid sequence are contiguous and in a sequential order.

According to preferred embodiments of the present invention, there is provided an isolated polypeptide encoding for a tail of T46984_PEA_(—)1_P3 (SEQ ID NO: 386), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence ICHIWKLIFLP (SEQ ID NO: 1061) in T46984_PEA_(—)1_P3 (SEQ ID NO: 386).

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for T46984_PEA_(—)1_P10 (SEQ ID NO: 387), comprising a first amino acid sequence being at least 90% homologous to MAPPGSSTVFLLALTIIASTWALTPTHYLTKHDVERLKASLDRPFTNLESAFYSIVGLSSL GAQVPDAKKACTYIRSNLDPSNVDSLFYAAQASQALSGCEISISNETKDLLLAAVSEDSS VTQIYHAVAALSGFGLPLASQEALSALTARLSKEETVLATVQALQTASHLSQQADLRSI VEEIEDLVARLDELGGVYLQFEEGLETTALFVAATYKLMDHVGTEPSIKEDQVIQLMNA IFSKKNFESLSEAFSVASAAAVLSHNRYHVPVVVVPEGSASDTHEQAILRLQVTNVLSQ PLTQATVKLEHAKSVASRATVLQKTSFTPVGDVFELNFMNVKFSSGYYDFLVEVEGDN RYIANTVELRVKISTEVGITNVDLSTVDKDQSIAPKTTRVTYPAKAKGTFIADSHQNFAL FFQLVDVNTGAELTPHQTFVRLHNQKTGQEVVFVAEPDNKNVYKFELDTSERKIEFDS ASGTYTLYLIIGDATLKNPILWNV corresponding to amino acids 1-498 of RIB2_HUMAN, which also corresponds to amino acids 1-498 of T46984_PEA_(—)1_P10 (SEQ ID NO: 387), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence LMDQK (SEQ ID NO: 1062) corresponding to amino acids 499-503 of T46984_PEA_(—)1_P10 (SEQ ID NO: 387), wherein said first amino acid sequence and second amino acid sequence are contiguous and in a sequential order.

According to preferred embodiments of the present invention, there is provided an isolated polypeptide encoding for a tail of T46984_PEA_(—)1_P10 (SEQ ID NO: 387), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence LMDQK (SEQ ID NO: 1062) in T46984_PEA_(—)1_P10 (SEQ ID NO: 387).

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for T46984_PEA_(—)1_P11 (SEQ ID NO: 388), comprising a first amino acid sequence being at least 90% homologous to MAPPGSSTVFLLALTIIASTWALTPTHYLTKHDVERLKASLDRPFTNLESAFYSIVGLSSL GAQVPDAKKACTYIRSNLDPSNVDSLFYAAQASQALSGCEISISNETKDLLLAAVSEDSS VTQIYHAVAALSGFGLPLASQEALSALTARLSKEETVLATVQALQTASHLSQQADLRSI VEEIEDLVARLDELGGVYLQFEEGLETTALFVAATYKLMDHVGTEPSIKEDQVIQLMNA IFSKKNFESLSEAFSVASAAAVLSHNRYHVPVVVVPEGSASDTHEQAILRLQVTNVLSQ PLTQATVKLEHAKSVASRATVLQKTSFTPVGDVFELNFMNVKFSSGYYDFLVEVEGDN RYIANTVELRVKISTEVGITNVDLSTVDKDQSIAPKTTRVTYPAKAKGTFIADSHQNFAL FFQLVDVNTGAELTPHQTFVRLHNQKTGQEVVFVAEPDNKNVYKFELDTSERKIEFDS ASGTYTLYLIIGDATLKNPILWNVADVVIKFPEEEAPSTVLSQNLFTPKQEIQHLFREPEK RPPTVVSNTFTALILSPLLLLFALWIRIGANVSNFTFAPSTIIFHLGHAAMLGLMYVYWT QLNMFQTLKYLAILGSVTFLAGNRMLAQQAVKR corresponding to amino acids 1-628 of RIB2_HUMAN, which also corresponds to amino acids 1-628 of T46984_PEA_(—)1_P11 (SEQ ID NO: 388).

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for T46984_PEA_(—)1_P12 (SEQ ID NO: 389), comprising a first amino acid sequence being at least 90% homologous to MAPPGSSTVFLLALTIIASTWALTPTHYLTKHDVERLKASLDRPFTNLESAFYSIVGLSSL GAQVPDAKKACTYIRSNLDPSNVDSLFYAAQASQALSGCEISISNETKDLLLAAVSEDSS VTQIYHAVAALSGFGLPLASQEALSALTARLSKEETVLATVQALQTASHLSQQADLRSI VEEIEDLVARLDELGGVYLQFEEGLETTALFVAATYKLMDHVGTEPSIKEDQVIQLMNA IFSKKNFESLSEAFSVASAAAVLSHNRYHVPVVVVPEGSASDTHEQAILRLQVTNVLSQ PLTQATVKLEHAKSVASRATVLQKTSFTPVGDVFELNFMN corresponding to amino acids 1-338 of RIB2_HUMAN, which also corresponds to amino acids 1-338 of T46984_PEA_(—)1_P12 (SEQ ID NO: 389), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence SQDLH (SEQ ID NO: 1063) corresponding to amino acids 339-343 of T46984_PEA_(—)1_P12 (SEQ ID NO: 389), wherein said first amino acid sequence and second amino acid sequence are contiguous and in a sequential order.

According to preferred embodiments of the present invention, there is provided an isolated polypeptide encoding for a tail of T46984_PEA_(—)1_P12 (SEQ ID NO: 389), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence SQDLH (SEQ ID NO: 1063) in T46984_PEA_(—)1_P12 (SEQ ID NO: 389).

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for T46984_PEA_(—)1_P21 (SEQ ID NO: 390), comprising a first amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence M corresponding to amino acids 1-1 of T46984_PEA_(—)1_P21 (SEQ ID NO: 390), and a second amino acid sequence being at least 90% homologous to KACTYIRSNLDPSNVDSLFYAAQASQALSGCEISISNETKDLLLAAVSEDSSVTQIYHAV AALSGFGLPLASQEALSALTARLSKEETVLATVQALQTASHLSQQADLRSIVEEIEDLVA RLDELGGVYLQFEEGLETTALFVAATYKLMDHVGTEPSIKEDQVIQLMNAIFSKKNFES LSEAFSVASAAAVLSHNRYHVPVVVVPEGSASDTHEQAILRLQVTNVLSQPLTQATVKL EHAKSVASRATVLQKTSFTPVGDVFELNFMNVKFSSGYYDFLVEVEGDNRYIANTVEL RVKISTEVGITNVDLSTVDKDQSIAPKTTRVTYPAKAKGTFIADSHQNFALFFQLVDVNT GAELTPHQTFVRLHNQKTGQEVVFVAEPDNKNVYKFELDTSERKIEFDSASGTYTLYLII GDATLKNPILWNVADVVIKFPEEEAPSTVLSQNLFTPKQEIQHLFREPEKRPPTVVSNTF TALILSPLLLLFALWIRIGANVSNFTFAPSTIIFHLGHAAMLGLMYVYWTQLNMFQTLKY LAILGSVTFLAGNRMLAQQAVKRTAH corresponding to amino acids 70-631 of RIB2_HUMAN, which also corresponds to amino acids 2-563 of T46984_PEA_(—)1_P21 (SEQ ID NO: 390), wherein said first amino acid sequence and second amino acid sequence are contiguous and in a sequential order.

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for T46984_PEA_(—)1_P27 (SEQ ID NO: 391), comprising a first amino acid sequence being at least 90% homologous to MAPPGSSTVFLLALTIIASTWALTPTHYLTKHDVERLKASLDRPFTNLESAFYSIVGLSSL GAQVPDAKKACTYIRSNLDPSNVDSLFYAAQASQALSGCEISISNETKDLLLAAVSEDSS VTQIYHAVAALSGFGLPLASQEALSALTARLSKEETVLATVQALQTASHLSQQADLRSI VEEIEDLVARLDELGGVYLQFEEGLETTALFVAATYKLMDHVGTEPSIKEDQVIQLMNA IFSKKNFESLSEAFSVASAAAVLSHNRYHVPVVVVPEGSASDTHEQAILRLQVTNVLSQ PLTQATVKLEHAKSVASRATVLQKTSFTPVGDVFELNFMNVKFSSGYYDFLVEVEGDN RYIANTVELRVKISTEVGITNVDLSTVDKDQSIAPKTTRVTYPAKAKGTFIADSHQNFA corresponding to amino acids 1-415 of RIB2_HUMAN, which also corresponds to amino acids 1-415 of T46984_PEA_(—)1_P27 (SEQ ID NO: 391), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence FGSGLVPMSPTSLLLLARLYFTWDMLLCWDSCMSTGLSSTCSRP (SEQ ID NO: 1064) corresponding to amino acids 416-459 of T46984_PEA_(—)1_P27 (SEQ ID NO: 391), wherein said first amino acid sequence and second amino acid sequence are contiguous and in a sequential order.

According to preferred embodiments of the present invention, there is provided an isolated polypeptide encoding for a tail of T46984_PEA_(—)1_P27 (SEQ ID NO: 391), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence FGSGLVPMSPTSLLLLARLYFTWDMLLCWDSCMSTGLSSTCSRP (SEQ ID NO: 1064) in T46984_PEA_(—)1_P27 (SEQ ID NO: 391).

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for T46984_PEA_(—)1_P32 (SEQ ID NO: 392), comprising a first amino acid sequence being at least 90% homologous to MAPPGSSTVFLLALTIIASTWALTPTHYLTKHDVERLKASLDRPFTNLESAFYSIVGLSSL GAQVPDAKKACTYIRSNLDPSNVDSLFYAAQASQALSGCEISISNETKDLLLAAVSEDSS VTQIYHAVAALSGFGLPLASQEALSALTARLSKEETVLATVQALQTASHLSQQADLRSI VEEIEDLVARLDELGGVYLQFEEGLETTALFVAATYKLMDHVGTEPSIKEDQVIQLMNA IFSKKNFESLSEAFSVASAAAVLSHNRYHVPVVVVPEGSASDTHEQAILRLQVTNVLSQ PLTQATVKLEHAKSVASRATVLQKTSFTPVGDVFELNFMNVKFSSGYYDFLVEVEGDN RYIANTVE corresponding to amino acids 1-364 of RIB2_HUMAN, which also corresponds to amino acids 1-364 of T46984_PEA_(—)1_P32 (SEQ ID NO: 392), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence GQVRWLTPVIPALWEAKAGGSPEVRSSILAWPT (SEQ ID NO: 1065) corresponding to amino acids 365-397 of T46984_PEA_(—)1_P32 (SEQ ID NO: 392), wherein said first amino acid sequence and second amino acid sequence are contiguous and in a sequential order.

According to preferred embodiments of the present invention, there is provided an isolated polypeptide encoding for a tail of T46984_PEA_(—)1_P32 (SEQ ID NO: 392), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence GQVRWLTPVIPALWEAKAGGSPEVRSSILAWPT (SEQ ID NO: 1065) in T46984_PEA_(—)1_P32 (SEQ ID NO: 392).

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for T46984_PEA_(—)1_P34 (SEQ ID NO: 393), comprising a first amino acid sequence being at least 90% homologous to MAPPGSSTVFLLALTIIASTWALTPTHYLTKHDVERLKASLDRPFTNLESAFYSIVGLSSL GAQVPDAKKACTYIRSNLDPSNVDSLFYAAQASQALSGCEISISNETKDLLLAAVSEDSS VTQIYHAVAALSGFGLPLASQEALSALTARLSKEETVLATVQALQTASHLSQQADLRSI VEEIEDLVARLDELGGVYLQFEEGLETTALFVAATYKLMDHVGTEPSIKEDQVIQLMNA IFSKKNFESLSEAFSVASAAAVLSHNRYHVPVVVVPEGSASDTHEQAILRLQVTNVLSQ PLTQATVKLEHAKSVASRATVLQKTSFTPVG corresponding to amino acids 1-329 of RIB2_HUMAN, which also corresponds to amino acids 1-329 of T46984_PEA_(—)1_P34 (SEQ ID NO: 393).

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for T46984_PEA_(—)1_P35 (SEQ ID NO: 394), comprising a first amino acid sequence being at least 90% homologous to MAPPGSSTVFLLALTIIASTWALTPTHYLTKHDVERLKASLDRPFTNLESAFYSIVGLSSL GAQVPDAKKACTYIRSNLDPSNVDSLFYAAQASQALSGCEISISNETKDLLLAAVSEDSS VTQIYHAVAALSGFGLPLASQEALSALTARLSKEETVLATVQALQTASHLSQQADLRSI VEEIEDLVARLDELGGVYLQFEEGLETTALFVAATYKLMDHVGTEPSIKEDQVIQLMNA IFSKKNFESLSEAFSVASAAAVLSHNRYHVPVVVVPEGSASDTHEQAI corresponding to amino acids 1-287 of RIB2_HUMAN, which also corresponds to amino acids 1-287 of T46984_PEA_(—)1_P35 (SEQ ID NO: 394), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence GCWPSRQSREQHISSRRKMEILKTECQEKESRTIHSMRRKMEKKNFI (SEQ ID NO: 1066) corresponding to amino acids 288-334 of T46984_PEA_(—)1_P35 (SEQ ID NO: 394), wherein said first amino acid sequence and second amino acid sequence are contiguous and in a sequential order.

According to preferred embodiments of the present invention, there is provided an isolated polypeptide encoding for a tail of T46984_PEA_(—)1_P35 (SEQ ID NO: 394), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence GCWPSRQSREQHISSRRKMEILKTECQEKESRTIHSMRRKMEKKNFI (SEQ ID NO: 1066) in T46984_PEA_(—)1_P35 (SEQ ID NO: 394).

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for T46984_PEA_(—)1_P38 (SEQ ID NO: 395), comprising a first amino acid sequence being at least 90% homologous to MAPPGSSTVFLLALTIIASTWALTPTHYLTKHDVERLKASLDRPFTNLESAFYSIVGLSSL GAQVPDAKKACTYIRSNLDPSNVDSLFYAAQASQALSGCEISISNETKDLLLAAVSEDSS VTQIYHAVAALSGFGLPLASQEAL corresponding to amino acids 1-145 of RIB2_HUMAN, which also corresponds to amino acids 1-145 of T46984_PEA_(—)1_P38 (SEQ ID NO: 395), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence MDPDWCQCLQLHFCS (SEQ ID NO: 1067) corresponding to amino acids 146-160 of T46984_PEA_(—)1_P38 (SEQ ID NO: 395), wherein said first amino acid sequence and second amino acid sequence are contiguous and in a sequential order.

According to preferred embodiments of the present invention, there is provided an isolated polypeptide encoding for a tail of T46984_PEA_(—)1_P38 (SEQ ID NO: 395), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence MDPDWCQCLQLHFCS (SEQ ID NO: 1067) in T46984_PEA_(—)1_P38 (SEQ ID NO: 395).

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for T46984_PEA_(—)1_P39 (SEQ ID NO. 396), comprising a first amino acid sequence being at least 90% homologous to MAPPGSSTVFLLALTIIASTWALTPTHYLTKHDVERLKASLDRPFTNLESAFYSIVGLSSL GAQVPDAKKACTYIRSNLDPSNVDSLFYAAQASQALSGCEISISNETKDLLLAAVSEDSS VTQIYHAVAALSGFGLPLASQEALSALTARLSKEETVLA corresponding to amino acids 1-160 of RIB2_HUMAN, which also corresponds to amino acids 1-160 of T46984_PEA_(—)1_P39 (SEQ ID NO. 396).

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for T46984_PEA_(—)1_P45 (SEQ ID NO: 397), comprising a first amino acid sequence being at least 90% homologous to MAPPGSSTVFLLALTIIASTWALTPTHYLTKHDVERLKASLDRPFTNLESAFYSIVGLSSL GAQVPDAKKACTYIRSNLDPSNVDSLFYAAQASQALSGCE corresponding to amino acids 1-101 of RIB2_HUMAN, which also corresponds to amino acids 1-101 of T46984_PEA_(—)1_P45 (SEQ ID NO: 397), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence NSPGSADSIPPVPAG (SEQ ID NO: 1068) corresponding to amino acids 102-116 of T46984_PEA_(—)1_P45 (SEQ ID NO: 397), wherein said first amino acid sequence and second amino acid sequence are contiguous and in a sequential order.

According to preferred embodiments of the present invention, there is provided an isolated polypeptide encoding for a tail of T46984_PEA_(—)1_P45 (SEQ ID NO: 397), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence NSPGSADSIPPVPAG (SEQ ID NO: 1068) in T46984_PEA_(—)1_P45 (SEQ ID NO: 397).

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for T46984_PEA_(—)1_P46 (SEQ ID NO: 398), comprising a first amino acid sequence being at least 90% homologous to MAPPQSSTVFLLALTIIASTWALTPTHYLTKHDVERLKASLDRPFTNLESAFYSIVGLSSL GAQVPDAK corresponding to amino acids 1-69 of RIB2_HUMAN, which also corresponds to amino acids 1-69 of T46984_PEA_(—)1_P46 (SEQ ID NO: 398), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence NSPGSADSIPPVPAG (SEQ ID NO: 1068) corresponding to amino acids 70-84 of T46984_PEA_(—)1_P46 (SEQ ID NO: 398), wherein said first amino acid sequence and second amino acid sequence are contiguous and in a sequential order.

According to preferred embodiments of the present invention, there is provided an isolated polypeptide encoding for a tail of T46984_PEA_(—)1_P46 (SEQ ID NO: 398), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence NSPGSADSIPPVPAG (SEQ ID NO: 1068) in T46984_PEA_(—)1_P46 (SEQ ID NO: 398).

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for M78530_PEA_(—)1_P15 (SEQ ID NO: 426), comprising a first amino acid sequence being at least 90% homologous to MRLSPAPLKLSRTPALLALALPLAAALAFSDETLDKVPKSEGYCSRILRAQGTRREGYT EFSLRVEGDPDFYKPGTSYRVTLSAAPPSYFRGFTLIALRENREGDKEEDHAGTFQIIDEE ETQFMSNCPVAVTESTPRRRTRIQVFWIAPPAGTGCVILKASIVQKRIIYFQDEGSLTKKL CEQDSTFDGVTDKPILDCCACGTAKYRLTFYGNWSEKTHPKDYPRRANHWSAIIGGSH SKNYVLWEYGGYASEGVKQVAELGSPVKMEEEIRQQSDEVLTVIKAKAQWPAWQPLN VRAAPSAEFSVDRTRHLMSFLTMMGPSPDWNVGLSAEDLCTKECGWVQKVVQDLIPW DAGTDSGVTYESPNKPTIPQEKIRPLTSLDHPQSPFYDPEGGSITQVARVVIERIARKGEQ CNIVPDNVDDIVADLAPEEKDEDDTPETCIYSNWSPWSACSSSTCDKGKRMRQRMLKA QLDLSVPCPDTQDFQPCMGPGCSDEDGSTCTMSEWITWSPCSISCGMGMRSRERYVKQ FPEDGSVCTLPTEE corresponding to amino acids 1-544 of Q9HCB6 (SEQ ID NO: 424), which also corresponds to amino acids 1-544 of M78530_PEA_(—)1_P15 (SEQ ID NO: 426), a bridging amino acid T corresponding to amino acid 545 of M78530_PEA_(—)1_P15 (SEQ ID NO: 426), a second amino acid sequence being at least 90% homologous to EKCTVNEECSPSSCLMTEWGEWDECSATCGMGMKKRHRMIKMNPADGSMCKAETSQ AEKCMMPECHTIPCLLSPWSEWSDCSVTCGKGMRTRQRMLKSLAELGDCNEDLEQVE KCMLPEC corresponding to amino acids 546-665 of Q9HCB6 (SEQ ID NO: 424), which also corresponds to amino acids 546-665 of M78530_PEA_(—)1_P15 (SEQ ID NO: 426), and a third amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence RKSWSSSRPITSMFLSPGSPEPASANTARS (SEQ ID NO: 1070) corresponding to amino acids 666-695 of M78530_PEA_(—)1_P15 (SEQ ID NO: 426), wherein said first amino acid sequence, bridging amino acid, second amino acid sequence and third amino acid sequence are contiguous and in a sequential order.

According to preferred embodiments of the present invention, there is provided an isolated polypeptide encoding for a tail of M78530_PEA_(—)1_P15 (SEQ ID NO: 426), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence RKSWSSSRPITSMFLSPGSPEPASANTARS (SEQ ID NO: 1070) in M78530_PEA_(—)1_P15 (SEQ ID NO: 426).

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for M78530_PEA_(—)1_P15 (SEQ ID NO: 426), comprising a first amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence MRLSPAPLKLSRTPALLALALPLAAALAFSDETLDKVPKSEGYCSRILRAQGTRREGYT EFSLRVEGDPDFYKPGTSYRVTLS (SEQ ID NO: 1071) corresponding to amino acids 1-83 of M78530_PEA_(—)1_P15 (SEQ ID NO: 426), a second amino acid sequence being at least 90% homologous to AAPPSYFRGFTLIALRENREGDKEEDHAGTFQIIDEEETQFMSNCPVAVTESTPRRRTRIQ VFWIAPPAGTGCVILKASIVQKRIIYFQDEGSLTKKLCEQDSTFDGVTDKPILDCCACGT AKYRLTFYGNWSEKTHPKDYPRRANHWSAIIGGSHSKNYVLWEYGGYASEGVKQVAE LGSPVKMEEEIRQQSDEVLTVIKAKAQWPAWQPLNVRAAPSAEFSVDRTRHLMSFLTM MGPSPDWNVGLSAEDLCTKECGWVQKVVQDLIPWDAGTDSGVTYESPNKPTIPQEKIR PLTSLDHPQSPFYDPEGGSITQVARVVIERIARKGEQCNIVPDNVDDIVADLAPEEKDED DTPETCIYSNWSPWSACSSSTCDKGKRMRQRMLKAQLDLSVPCPDTQDFQPCMGPGCS DEDGSTCTMSEWITWSPCSISCGMGMRSRERYVKQFPEDGSVCTLPTEETEKCTVNEEC SPSSCLMTEWGEWDECSATCGMGMKKRHRMIKMPADGSMCKAETSQAEKCMMPE CHTIPCLLSPWSEWSDCSVTCGKGMRTRQRMLKSLAELGDCNEDLEQVEKCMLPEC corresponding to amino acids 1-582 of O94862 (SEQ ID NO: 425), which also corresponds to amino acids 84-665 of M78530_PEA_(—)1_P15 (SEQ ID NO: 426), and a third amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence RKSWSSSRPITSMFLSPGSPEPASANTARS (SEQ ID NO: 1070) corresponding to amino acids 666-695 of M78530_PEA_(—)1_P15 (SEQ ID NO: 426), wherein said first amino acid sequence, second amino acid sequence and third amino acid sequence are contiguous and in a sequential order.

According to preferred embodiments of the present invention, there is provided an isolated polypeptide encoding for a head of M78530_PEA_(—)1_P15 (SEQ ID NO: 426), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence MRLSPAPLKLSRTPALLALALPLAAALAFSDETLDKVPKSEGYCSRILRAQGTRREGYT EFSLRVEGDPDFYKPGTSYRVTLS (SEQ ID NO: 1071) of M78530_PEA_(—)1_P15 (SEQ ID NO: 426).

An isolated polypeptide encoding for a tail of M78530_PEA_(—)1_P15 (SEQ ID NO: 426), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence RKSWSSSRPITSMFLSPGSPEPASANTARS (SEQ ID NO: 1070) in M78530_PEA_(—)1_P15 (SEQ ID NO: 426).

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for M78530_PEA_(—)1_P16 (SEQ ID NO: 427), comprising a first amino acid sequence being at least 90% homologous to MRLSPAPLKLSRTPALLALALPLAAALAFSDETLDKVPKSEGYCSRILRAQGTRREGYT EFSLRVEGDPDFYKPGTSYRVTLSAAPPSYFRGFTLIALRENREGDKEEDHAGTFQIIDEE ETQFMSNCPVAVTESTPRRRTRIQVFWIAPPAGTGCVILKASIVQKRIIYFQDEGSLTKKL CEQDSTFDGVTDKPILDCCACGTAKYRLTFYGNWSEKTHPKDYPRRANHWSAIIGGSH SKNYVLWEYGGYASEGVKQVAELGSPVKMEEEIRQQSDEVLTVIKAKAQWPAWQPLN V corresponding to amino acids 1-297 of Q8NCD7 (SEQ ID NO: 423), which also corresponds to amino acids 1-297 of M78530_PEA_(—)1_P16 (SEQ ID NO: 427).

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for M78530_PEA_(—)1_P16 (SEQ ID NO: 427), comprising a first amino acid sequence being at least 90% homologous to MRLSPAPLKLSRTPALLALALPLAAALAFSDETLDKVPKSEGYCSRILRAQGTRREGYT EFSLRVEGDPDFYKPGTSYRVTLSAAPPSYFRGFTLIALRENREGDKEEDHAGTFQIIDEE ETQFMSNCPVAVTESTPRRRTRIQVFWIAPPAGTGCVILKASIVQKRIIYFQDEGSLTKKL CEQDSTFDGVTDKPILDCCACGTAKYRLTFYGNWSEKTHPKDYPRRANHWSAIIGGSH SKNYVLWEYGGYASEGVKQVAELGSPVKMEEEIRQQSDEVLTVIKAKAQWPAWQPLN V corresponding to amino acids 1-297 of Q9HCB6 (SEQ ID NO: 424), which also corresponds to amino acids 1-297 of M78530_PEA_(—)1_P16 (SEQ ID NO: 427).

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for M78530_PEA_(—)1_P16 (SEQ ID NO: 427), comprising a first amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence MRLSPAPLKLSRTPALLALALPLAAALAFSDETLDKVPKSEGYCSRILRAQGTRREGYT EFSLRVEGDPDFYKPGTSYRVTLS (SEQ ID NO: 1071) corresponding to amino acids 1-83 of M78530_PEA_(—)1_P16 (SEQ ID NO: 427), and a second amino acid sequence being at least 90% homologous to AAPPSYFRGFTLIALRENREGDKEEDHAGTFQIIDEEETQFMSNCPVAVTESTPRRRTRIQ VFWIAPPAGTGCVILKASIVQKRIIYFQDEGSLTKKLCEQDSTFDGVTDKPILDCCACGT AKYRLTFYGNWSEKTHPKDYPRRANHWSAIIGGSHSKNYVLWEYGGYASEGVKQVAE LGSPVKMEEEIRQQSDEVLTVIKAKAQWPAWQPLNV corresponding to amino acids 1-214 of O94862 (SEQ ID NO: 425), which also corresponds to amino acids 84-297 of M78530_PEA_(—)1_P16 (SEQ ID NO: 427), wherein said first amino acid sequence and second amino acid sequence are contiguous and in a sequential order.

According to preferred embodiments of the present invention, there is provided an isolated polypeptide encoding for a head of M78530_PEA_(—)1_P16 (SEQ ID NO: 427), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence MRLSPAPLKLSRTPALLALALPLAAALAFSDETLDKVPKSEGYCSRILRAQGTRREGYT EFSLRVEGDPDFYKPGTSYRVTLS (SEQ ID NO: 1071) of M78530_PEA_(—)1_P16 (SEQ ID NO: 427).

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for M78530_PEA_(—)1_P17 (SEQ ID NO: 428), comprising a first amino acid sequence being at least 90% homologous to MRLSPAPLKLSRTPALLALALPLAAALAFSDETLDKVPKSEGYCSRILRAQGTRREGYT EFSLRVEGDPDFYKPGTSYRVTLSAAPPSYFRGFTLIALRENREGDKEEDHAGTFQIIDEE ETQFMSNCPVAVTESTPRRRTRIQVFWIAPPAGTGCVILKASIVQKRIIYFQDEGSLTKKL CEQDSTFDGVTDKPILDCCACGTAKYRLTFYGNWSEKTHPKDYPRRANHWSAIIGGSH SKNYVLWEYGGYASEGVKQVAELGSPVKMEEEIRQQ corresponding to amino acids 1-275 of Q8NCD7 (SEQ ID NO: 423), which also corresponds to amino acids 1-275 of M78530_PEA_(—)1_P17 (SEQ ID NO: 428), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence VRQKNHRMTK (SEQ ID NO: 1073) corresponding to amino acids 276-285 of M78530_PEA_(—)1_P17 (SEQ ID NO: 428), wherein said first amino acid sequence and second amino acid sequence are contiguous and in a sequential order.

According to preferred embodiments of the present invention, there is provided an isolated polypeptide encoding for a tail of M78530_PEA_(—)1_P17 (SEQ ID NO: 428), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence VRQKNHRMTK (SEQ ID NO: 1073) in M78530_PEA_(—)1_P17 (SEQ ID NO: 428).

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for M78530_PEA_(—)1_P17 (SEQ ID NO: 428), comprising a first amino acid sequence being at least 90% homologous to MRLSPAPLKLSRTPALLALALPLAAALAFSDETLDKVPKSEGYCSRILRAQGTRREGYT EFSLRVEGDPDFYKPGTSYRVTLSAAPPSYFRGFTLIALRENREGDKEEDHAGTFQIIDEE ETQFMSNCPVAVTESTPRRRTRIQVFWIAPPAGTGCVILKASIVQKRIIYFQDEGSLTKKL CEQDSTFDGVTDKPILDCCACGTAKYRLTFYGNWSEKTHPKDYPRRANHWSAIIGGSH SKNYVLWEYGGYASEGVKQVAELGSPVKMEEEIRQQ corresponding to amino acids 1-275 of Q9HCB6 (SEQ ID NO: 424), which also corresponds to amino acids 1-275 of M78530_PEA_(—)1_P17 (SEQ ID NO: 428), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence VRQKNHRMTK (SEQ ID NO: 1073) corresponding to amino acids 276-285 of M78530_PEA_(—)1_P17 (SEQ ID NO: 428), wherein said first amino acid sequence and second amino acid sequence are contiguous and in a sequential order.

According to preferred embodiments of the present invention, there is provided an isolated polypeptide encoding for a tail of M78530_PEA_(—)1_P17 (SEQ ID NO: 428), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence VRQKNHRMTK (SEQ ID NO: 1073) in M78530_PEA_(—)1_P17 (SEQ ID NO: 428).

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for M78530_PEA_(—)1_P17 (SEQ ID NO: 428), comprising a first amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence MRLSPAPLKLSRTPALLALALPLAAALAFSDETLDKVPKSEGYCSRILRAQGTRREGYT EFSLRVEGDPDFYKPGTSYRVTLS (SEQ ID NO: 1071) corresponding to amino acids 1-83 of M78530_PEA_(—)1_P17 (SEQ ID NO: 428), a second amino acid sequence being at least 90% homologous to AAPPSYFRGFTLIALRENREGDKEEDHAGTFQIIDEEETQFMSNCPVAVTESTPRRRTRIQ VFWIAPPAGTGCVILKASIVQKRIIYFQDEGSLTKKLCEQDSTFDGVTDKPILDCCACGT AKYRLTFYGNWSEKTHPKDYPRRANHWSAIIGGSHSKNYVLWEYGGYASEGVKQVAE LGSPVKMEEEIRQQ corresponding to amino acids 1-192 of O94862 (SEQ ID NO: 425), which also corresponds to amino acids 84-275 of M78530_PEA_(—)1_P17 (SEQ ID NO: 428), and a third amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence VRQKNHRMTK (SEQ ID NO: 1073) corresponding to amino acids 276-285 of M78530_PEA_(—)1_P17 (SEQ ID NO: 428), wherein said first amino acid sequence, second amino acid sequence and third amino acid sequence are contiguous and in a sequential order.

According to preferred embodiments of the present invention, there is provided an isolated polypeptide encoding for a head of M78530_PEA_(—)1_P17 (SEQ ID NO: 428), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence MRLSPAPLKLSRTPALLALALPLAAALAFSDETLDKVPKSEGYCSRILRAQGTRREGYT EFSLRVEGDPDFYKPGTSYRVTLS (SEQ ID NO: 1071) of M78530_PEA_(—)1_P17 (SEQ ID NO: 428).

According to preferred embodiments of the present invention, there is provided an isolated polypeptide encoding for a tail of M78530_PEA_(—)1_P17 (SEQ ID NO: 428), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence VRQKNHRMTK (SEQ ID NO: 1073) in M78530_PEA_(—)1_P17 (SEQ ID NO: 428).

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for T48119_P2 (SEQ ID NO: 450), comprising a first amino acid sequence being at least 90% homologous to MTRQMASSGASGGKIDNSVLVLIVGLSTVGAGAYAYKTMKEDEKRYNERISGLGLTPE QKQKKAALSASEGEEVPQDKAPSHVPFLLIGGGTAAFAAARSIRARDPGARVLIVSEDP ELPYMRPPLSKELWFSDDPNVTKTLRFKQWNGKERSIYFQPPSFYVSAQDLPHIENGGV AVLTGKKVVQLDVRDNMVKLNDGSQITYEKCLIATGGTPRSLSAIDRAGAEVKSRTTL FRKIGDFRSLEKISREVKSITIIGGGFLGSELACALGRKARALGTEVIQLFPEKGNMGKILP EYLSNWTMEKVRREGVKVMPNAIVQSVGVSSGKLLIKLKDGRKVETDHIVAAVGLEP NVELAKTGGLEIDSDFGGFRVNAELQARSNIWVAGDAACFYDIKLGRRRVEHHDHAV VSGRLAGENMTGAAKPYWHQSMFWSDLGPDVGYEAIGLVDSSLPTVGVFAKATAQD NPKSATEQSGTGIRSESETESEASEITIPPSTPAVPQAPVQGEDYGKGVIFYLRDKVVVGI VLWNIFNRMPIARKIIKDGEQHEDLNEVAKLFNIHED corresponding to amino acids 50-613 of PCD8_HUMAN, which also corresponds to amino acids 1-564 of T48119_P2 (SEQ ID NO: 450).

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for T48119_P2 (SEQ ID NO: 450), comprising a first amino acid sequence being at least 90% homologous to MTRQMASSGASGGKIDNSVLVLIVGLSTVGAGAYAYKTMKEDEKRYNERISGLGLTPE QKQKKAALSASEGEEVPQDKAPSHVPFLLIGGGTAAFAAARSIRARDPGARVLIVSEDP ELPYMRPPLSKELWFSDDPNVTKTLRFKQWNGKERSIYFQPPSFYVSAQDLPHIENGGV AVLTGKKVVQLDVRDNMVKLNDGSQITYEKCLIATGGTPRSLSAIDRAGAEVKSRTTL FRKIGDFRSLEKISREVKSITIIGGGFLGSELACALGRKARALGTEVIQLFPEKGNMGKILP EYLSNWTMEKVRREGVKVMPNAIVQSVGVSSGKLLIKLKDGRKVETDHIVAAVGLEP NVELAKTGGLEIDSDFGGFRVNAELQARSNIWVAGDAACFYDIKLGRRRVEHHDHAV VSGRLAGENMTGAAKPYWHQSMFWSDLGPDVGYEAIGLVDSSLPTVGVFAKATAQD NPKSATEQSGTGIRSESETESEASEITIPPSTPAVPQAPVQGEDYGKGVIFYLRDKVVVGI VLWNIFNRMPIARKIIKDGEQHEDLNEVAKLFNIHED corresponding to amino acids 50-613 of PCD8_HUMAN, which also corresponds to amino acids 1-564 of T48119_P2 (SEQ ID NO: 450).

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for T39971_P6 (SEQ ID NO: 603), comprising a first amino acid sequence being at least 90% homologous to MAPLRPLLILALLAWVALADQESCKGRCTEGFNVDKKCQCDELCSYYQSCCTDYTAEC KPQVTRGDVFTMPEDEYTVYDDGEEKNNATVHEQVGGPSLTSDLQAQSKGNPEQTPV LKPEEEAPAPEVGASKPEGIDSRPETLHPGRPQPPAEEELCSGKPFDAFTDLKNGSLFAFR GQYCYELDEKAVRPGYPKLIRDVWGIEGPIDAAFTRINCQGKTYLFKGSQYWRFEDGV LDPDYPRNISDGFDGIPDNVDAALALPAHSYSGRERVYFFKG corresponding to amino acids 1-276 of VTNC_HUMAN, which also corresponds to amino acids 1-276 of T39971_P6 (SEQ ID NO: 603), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence TQGVVGD corresponding to amino acids 277-283 of T39971_P6 (SEQ ID NO: 603), wherein said first and second amino acid sequences are contiguous and in a sequential order.

According to preferred embodiments of the present invention, there is provided an isolated polypeptide encoding for a tail of T39971_P6 (SEQ ID NO: 603), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence TQGVVGD in T39971_P6 (SEQ ID NO: 603).

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for T39971_P9 (SEQ ID NO: 604), comprising a first amino acid sequence being at least 90% homologous to MAPLRPLLILALLAWVALADQESCKGRCTEGFNVDKKCQCDELCSYYQSCCTDYTAEC KPQVTRGDVFTMPEDEYTVYDDGEEKNNATVHEQVGGPSLTSDLQAQSKGNPEQTPV LKPEEEAPAPEVGASKPEGIDSRPETLHPGRPQPPAEEELCSGKPFDAFTDLKNGSLFAFR GQYCYELDEKAVRPGYPKLIRDVWGIEGPIDAAFTRINCQGKTYLFKGSQYWRFEDGV LDPDYPRNISDGFDGIPDNVDAALALPAHSYSGRERVYFFKGKQYWEYQFQHQPSQEE CEGSSLSAVFEHFAMMQRDSWEDIFELLFWGRT corresponding to amino acids 1-325 of VTNC_HUMAN, which also corresponds to amino acids 1-325 of T39971_P9 (SEQ ID NO: 604), and a second amino acid sequence being at least 90% homologous to SGMAPRPSLAKKQRFRHRNRKGYRSQRGHSRGRNQNSRRPSRATWLSLFSSEESNLGA NNYDDYRMDWLVPATCEPIQSVFFFSGDKYYRVNLRTRRVDTVDPPYPRSIAQYWLGC PAPGHL corresponding to amino acids 357-478 of VTNC_HUMAN, which also corresponds to amino acids 326-447 of T39971_P9 (SEQ ID NO: 604), wherein said first and second amino acid sequences are contiguous and in a sequential order.

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for an edge portion of T39971_P9 (SEQ ID NO: 604), comprising a polypeptide having a length “n”, wherein n is at least about 10 amino acids in length, optionally at least about 20 amino acids in length, preferably at least about 30 amino acids in length, more preferably at least about 40 amino acids in length and most preferably at least about 50 amino acids in length, wherein at least two amino acids comprise TS, having a structure as follows: a sequence starting from any of amino acid numbers 325-x to 325; and ending at any of amino acid numbers 326+((n−2)−x), in which x varies from 0 to n−2.

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for T39971_P11 (SEQ ID NO: 605), comprising a first amino acid sequence being at least 90% homologous to MAPLRPLLILALLAWVALADQESCKGRCTEGFNVDKKCQCDELCSYYQSCCTDYTAEC KPQVTRGDVFTMPEDEYTVYDDGEEKNNATVHEQVGGPSLTSDLQAQSKGNPEQTPV LKPEEEAPAPEVGASKPEGIDSRPETLHPGRPQPPAEEELCSGKPFDAFTDLKNGSLFAFR GQYCYELDEKAVRPGYPKLIRDVWGIEGPIDAAFTRINCQGKTYLFKGSQYWRFEDGV LDPDYPRNISDGFDGIPDNVDAALALPAHSYSGRERVYFFKGKQYWEYQFQHQPSQEE CEGSSLSAVFEHFAMMQRDSWEDIFELLFWGRTS corresponding to amino acids 1-326 of VTNC_HUMAN, which also corresponds to amino acids 1-326 of T39971_P11 (SEQ ID NO: 605), and a second amino acid sequence being at least 90% homologous to DKYYRVNLRTRRVDTVDPPYPRSIAQYWLGCPAPGHL corresponding to amino acids 442-478 of VTNC_HUMAN, which also corresponds to amino acids 327-363 of T39971_P11 (SEQ ID NO: 605), wherein said first and second amino acid sequences are contiguous and in a sequential order.

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for an edge portion of T39971_P11 (SEQ ID NO: 605), comprising a polypeptide having a length “n”, wherein n is at least about 10 amino acids in length, optionally at least about 20 amino acids in length, preferably at least about 30 amino acids in length, more preferably at least about 40 amino acids in length and most preferably at least about 50 amino acids in length, wherein at least two amino acids comprise SD, having a structure as follows: a sequence starting from any of amino acid numbers 326-x to 326; and ending at any of amino acid numbers 327+((n−2)−x), in which x varies from 0 to n−2.

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for T39971_P11 (SEQ ID NO: 605), comprising a first amino acid sequence being at least 90% homologous to MAPLRPLLILALLAWVALADQESCKGRCTEGFNVDKKCQCDELCSYYQSCCTDYTAEC KPQVTRGDVFTMPEDEYTVYDDGEEKNNATVHEQVGGPSLTSDLQAQSKGNPEQTPV LKPEEEAPAPEVGASKPEGIDSRPETLHPGRPQPPAEEELCSGKPFDAFTDLKNGSLFAFR GQYCYELDEKAVRPGYPKLIRDVWGIEGPIDAAFTRINCQGKTYLFKGSQYWRFEDGV LDPDYPRNISDGFDGIPDNVDAALALPAHSYSGRERVYFFKGKQYWEYQFQHQPSQEE CEGSSLSAVFEHFAMMQRDSWEDIFELLFWGRTS corresponding to amino acids 1-326 of Q9BSH7 (SEQ ID NO: 1000), which also corresponds to amino acids 1-326 of T39971_P11 (SEQ ID NO: 605), and a second amino acid sequence being at least 90% homologous to DKYYRVNLRTRRVDTVDPPYPRSIAQYWLGCPAPGHL corresponding to amino acids 442-478 of Q9BSH7 (SEQ ID NO: 1000), which also corresponds to amino acids 327-363 of T39971_P11 (SEQ ID NO: 605), wherein said first and second amino acid sequences are contiguous and in a sequential order.

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for an edge portion of T39971_P11 (SEQ ID NO: 605), comprising a polypeptide having a length “n”, wherein n is at least about 10 amino acids in length, optionally at least about 20 amino acids in length, preferably at least about 30 amino acids in length, more preferably at least about 40 amino acids in length and most preferably at least about 50 amino acids in length, wherein at least two amino acids comprise SD, having a structure as follows: a sequence starting from any of amino acid numbers 326-x to 326; and ending at any of amino acid numbers 327+((n−2)−x), in which x varies from 0 to n−2.

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for T39971_P12 (SEQ ID NO: 606), comprising a first amino acid sequence being at least 90% homologous to MAPLRPLLILALLAWVALADQESCKGRCTEGFNVDKKCQCDELCSYYQSCCTDYTAEC KPQVTRGDVFTMPEDEYTVYDDGEEKNNATVHEQVGGPSLTSDLQAQSKGNPEQTPV LKPEEEAPAPEVGASKPEGIDSRPETLHPGRPQPPAEEELCSGKPFDAFTDLKNGSLFAFR GQYCYELDEKAVRPGYPKLIRDVWGIEGPIDAAFTRINCQGKTYLFK corresponding to amino acids 1-223 of VTNC_HUMAN, which also corresponds to amino acids 1-223 of T39971_P12 (SEQ ID NO: 606), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence VPGAVGQGRKHLGRV (SEQ ID NO: 1076) corresponding to amino acids 224-238 of T39971_P12 (SEQ ID NO: 606), wherein said first and second amino acid sequences are contiguous and in a sequential order.

According to preferred embodiments of the present invention, there is provided an isolated polypeptide encoding for a tail of T39971_P12 (SEQ ID NO: 606), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence VPGAVGQGRKHLGRV (SEQ ID NO: 1076) in T39971_P12 (SEQ ID NO: 606).

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for T39971_P12 (SEQ ID NO: 606), comprising a first amino acid sequence being at least 90% homologous to MAPLRPLLILALLAWVALADQESCKGRCTEGFNVDKKCQCDELCSYYQSCCTDYTAEC KPQVTRGDVFTMPEDEYTVYDDGEEKNNATVHEQVGGPSLTSDLQAQSKGNPEQTPV LKPEEEAPAPEVGASKPEGIDSRPETLHPGRPQPPAEEELCSGKPFDAFTDLKNGSLFAFR GQYCYELDEKAVRPGYPKLIRDVWGIEGPIDAAFTRINCQGKTYLFK corresponding to amino acids 1-223 of Q9BSH7 (SEQ ID NO: 1000), which also corresponds to amino acids 1-223 of T39971_P12 (SEQ ID NO: 606), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence VPGAVGQGRKHLGRV (SEQ ID NO: 1076) corresponding to amino acids 224-238 of T39971_P12 (SEQ ID NO: 606), wherein said first and second amino acid sequences are contiguous and in a sequential order.

According to preferred embodiments of the present invention, there is provided an isolated polypeptide encoding for a tail of T39971_P12 (SEQ ID NO: 606), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence VPGAVGQGRKHLGRV (SEQ ID NO: 1076) in T39971_P12 (SEQ ID NO: 606).

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for Z44808_PEA_(—)1_P5 (SEQ ID NO: 634), comprising a first amino acid sequence being at least 90% homologous to MLLPQLCWLPLLAGLLPPVPAQKFSALTFLRVDQDKDKDCSLDCAGSPQKPLCASDGR TFLSRCEFQRAKCKDPQLEIAYRGNCKDVSRCVAERKYTQEQARKEFQQVFIPECNDD GTYSQVQCHSYTGYCWCVTPNGRPISGTAVAHKTPRCPGSVNEKLPQREGTGKTDDAA APALETQPQGDEEDIASRYPTLWTEQVKSRQNKTNKNSVSSCDQEHQSALEEAKQPKN DNVVIPECAHGGLYKPVQCHPSTGYCWCVLVDTGRPIPGTSTRYEQPKCDNTARAHPA KARDLYKGRQLQGCPGAKKHEFLTSVLDALSTDMVHAASDPSSSSGRLSEPDPSHTLEE RVVHWYFKLLDKNSSGDIGKKEIKPFKRFLRKKSKPKKCVKKFVEYCDVNNDKSISVQ ELMGCLGVAKEDGKADTKKRHTPRGHAESTSNRQ corresponding to amino acids 1-441 of SMO2_HUMAN, which also corresponds to amino acids 1-441 of Z44808_PEA_(—)1_P5 (SEQ ID NO: 634), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence DAMVVSSRPKATTHRKSRTLSRR (SEQ ID NO: 1077) corresponding to amino acids 442-464 of Z44808_PEA_(—)1_P5 (SEQ ID NO: 634), wherein said first and second amino acid sequences are contiguous and in a sequential order.

According to preferred embodiments of the present invention, there is provided an isolated polypeptide encoding for a tail of Z44808_PEA_(—)1_P5 (SEQ ID NO: 634), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence DAMVVSSRPKATTHRKSRTLSRR (SEQ ID NO: 1077) in Z44808_PEA_(—)1_P5 (SEQ ID NO: 634).

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for Z44808_PEA_(—)1_P6 (SEQ ID NO: 635), comprising a first amino acid sequence being at least 90% homologous to MLLPQLCWLPLLAGLLPPVPAQKFSALTFLRVDQDKDKDCSLDCAGSPQKPLCASDGR TFLSRCEFQRAKCKDPQLEIAYRGNCKDVSRCVAERKYTQEQARKEFQQVFIPECNDD GTYSQVQCHSYTGYCWCVTPNGRPISGTAVAHKTPRCPGSVNEKLPQREGTGKTDDAA APALETQPQGDEEDIASRYPTLWTEQVKSRQNKTNKNSVSSCDQEHQSALEEAKQPKN DNVVIPECAHGGLYKPVQCHPSTGYCWCVLVDTGRPIPGTSTRYEQPKCDNTARAHPA KARDLYKGRQLQGCPGAKKHEFLTSVLDALSTDMVHAASDPSSSSGRLSEPDPSHTLEE RVVHWYFKLLDKNSSGDIGKKEIKPFKRFLRKKSKPKKCVKKFVEYCDVNNDKSISVQ ELMGCLGVAKEDGKADTKKRH corresponding to amino acids 1-428 of SMO2_HUMAN, which also corresponds to amino acids 1-428 of Z44808_PEA_(—)1_P6 (SEQ ID NO: 635), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence RSKRNL (SEQ ID NO: 1078) corresponding to amino acids 429-434 of Z44808_PEA_(—)1_P6 (SEQ ID NO: 635), wherein said first and second amino acid sequences are contiguous and in a sequential order.

According to preferred embodiments of the present invention, there is provided an isolated polypeptide encoding for a tail of Z44808_PEA_(—)1_P6 (SEQ ID NO: 635), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence RSKRNL (SEQ ID NO: 1078) in Z44808_PEA_(—)1_P6 (SEQ ID NO: 635).

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for Z44808_PEA_(—)1_P7 (SEQ ID NO: 636), comprising a first amino acid sequence being at least 90% homologous to MLLPQLCWLPLLAGLLPPVPAQKFSALTFLRVDQDKDKDCSLDCAGSPQKPLCASDGR TFLSRCEFQRAKCKDPQLEIAYRGNCKDVSRCVAERKYTQEQARKEFQQVFIPECNDD GTYSQVQCHSYTGYCWCVTPNGRPISGTAVAHKTPRCPGSVNEKLPQREGTGKTDDAA APALETQPQGDEEDIASRYPTLWTEQVKSRQNKTNKNSVSSCDQEHQSALEEAKQPKN DNVVIPECAHGGLYKPVQCHPSTGYCWCVLVDTGRPIPGTSTRYEQPKCDNTARAHPA KARDLYKGRQLQGCPGAKKHEFLTSVLDALSTDMVHAASDPSSSSGRLSEPDPSHTLEE RVVHWYFKLLDKNSSGDIGKKEIKPFKRFLRKKSKPKKCVKKFVEYCDVNNDKSISVQ ELMGCLGVAKEDGKADTKKRHTPRGHAESTSNRQ corresponding to amino acids 1-441 of SMO2_HUMAN, which also corresponds to amino acids 1-441 of Z44808_PEA_(—)1_P7 (SEQ ID NO: 636), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence LLWLRGKVSFYCF (SEQ ID NO: 1079) corresponding to amino acids 442-454 of Z44808_PEA_(—)1_P7 (SEQ ID NO: 636), wherein said first and second amino acid sequences are contiguous and in a sequential order.

According to preferred embodiments of the present invention, there is provided an isolated polypeptide encoding for a tail of Z44808_PEA_(—)1_P7 (SEQ ID NO: 636), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence LLWLRGKVSFYCF (SEQ ID NO: 1079) in Z44808_PEA_(—)1_P7 (SEQ ID NO: 636).

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for Z44808_PEA_(—)1_P11 (SEQ ID NO: 637), comprising a first amino acid sequence being at least 90% homologous to MLLPQLCWLPLLAGLLPPVPAQKFSALTFLRVDQDKDKDCSLDCAGSPQKPLCASDGR TFLSRCEFQRAKCKDPQLEIAYRGNCKDVSRCVAERKYTQEQARKEFQQVFIPECNDD GTYSQVQCHSYTGYCWCVTPNGRPISGTAVAHKTPRCPGSVNEKLPQREGTGKT corresponding to amino acids 1-170 of SMO2_HUMAN, which also corresponds to amino acids 1-170 of Z44808_PEA_(—)1_P11 (SEQ ID NO: 637), and a second amino acid sequence being at least 90% homologous to DIASRYPTLWTEQVKSRQNKTNKNSVSSCDQEHQSALEEAKQPKNDNVVIPECAHGGL YKPVQCHPSTGYCWCVLVDTGRPIPGTSTRYEQPKCDNTARAHPAKARDLYKGRQLQ GCPGAKKHEFLTSVLDALSTDMVHAASDPSSSSGRLSEPDPSHTLEERVVHWYFKLLD KNSSGDIGKKEIKPFKRFLRKKSKPKKCVKKFVEYCDVNNDKSISVQELMGCLGVAKE DGKADTKKRHTPRGHAESTSNRQPRKQG corresponding to amino acids 188-446 of SMO2_HUMAN, which also corresponds to amino acids 171-429 of Z44808_PEA_(—)1_P11 (SEQ ID NO: 637), wherein said first and second amino acid sequences are contiguous and in a sequential order.

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for an edge portion of Z44808_PEA_(—)1_P11 (SEQ ID NO: 637), comprising a polypeptide having a length “n”, wherein n is at least about 10 amino acids in length, optionally at least about 20 amino acids in length, preferably at least about 30 amino acids in length, more preferably at least about 40 amino acids in length and most preferably at least about 50 amino acids in length, wherein at least two amino acids comprise TD, having a structure as follows: a sequence starting from any of amino acid numbers 170-x to −170; and ending at any of amino acid numbers 171+((n−2)−x), in which x varies from 0 to n−2.

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for S67314_PEA_(—)1_P4 (SEQ ID NO: 651), comprising a first amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence MVDAFLGTWKLVDSKNFDDYMKSLGVGFATRQVASMTKPTTIIEKNGDILTLKTHSTF KNTEISFKLGVEFDETTADDRKVKSIVTLDGGKLVHLQKWDGQETTLVRELIDGKLIL corresponding to amino acids 1-116 of FABH_HUMAN, which also corresponds to amino acids 1-116 of S67314_PEA_(—)1_P4 (SEQ ID NO: 651), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence VRWATLELYLIGYYYCSFSQACSKKPSPPLRAVEAGTREWLWVRVVSGGNFLCSGFGL TQAGTQILPYRLHDCGQITFSKCNCKTGINNTNLVGLLGSL (SEQ ID NO: 1080) corresponding to amino acids 117-215 of S67314_PEA_(—)1_P4 (SEQ ID NO: 651), wherein said first and second amino acid sequences are contiguous and in a sequential order.

According to preferred embodiments of the present invention, there is provided an isolated polypeptide encoding for a tail of S67314_PEA_(—)1_P4 (SEQ ID NO: 651), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence VRWATLELYLIGYYYCSFSQACSKKPSPPLRAVEAGTREWLWVRVVSGGNFLCSGFGL TQAGTQILPYRLHDCGQITFSKCNCKTGINNTNLVGLLGSL (SEQ ID NO: 1080) in S67314_PEA_(—)1_P4 (SEQ ID NO: 651).

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for S67314_PEA_(—)1_P4 (SEQ ID NO: 651), comprising a first amino acid sequence being at least 90% homologous to MVDAFLGTWKLVDSKNFDDYMKSLGVGFATRQVASMTKPTTIIEKNGDILTLKTHSTF KNTEISFKLGVEFDETTADDRKVKSIVTLDGGKLVHLQKWDGQETTLVRELIDGKLIL corresponding to amino acids 1-116 of AAP35373 (SEQ ID NO: 1007), which also corresponds to amino acids 1-116 of S67314_PEA_(—)1_P4 (SEQ ID NO: 651), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence VRWATLELYLIGYYYCSFSQACSKKPSPPLRAVEAGTREWLWVRVVSGGNFLCSGFGL TQAGTQILPYRLHDCGQITFSKCNCKTGINNTNLVGLLGSL (SEQ ID NO: 1080) corresponding to amino acids 117-215 of S67314_PEA_(—)1_P4 (SEQ ID NO: 651), wherein said first and second amino acid sequences are contiguous and in a sequential order.

According to preferred embodiments of the present invention, there is provided an isolated polypeptide encoding for a tail of S67314_PEA_(—)1_P4 (SEQ ID NO: 651), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence VRWATLELYLIGYYYCSFSQACSKKPSPPLRAVEAGTREWLWVRVVSGGNFLCSGFGL TQAGTQILPYRLHDCGQITFSKCNCKTGINNTNLVGLLGSL (SEQ ID NO: 1080) in S67314_PEA_(—)1_P4 (SEQ ID NO: 651).

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for S67314_PEA_(—)1_P5 (SEQ ID NO: 652), comprising a first amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence MVDAFLGTWKLVDSKNFDDYMKSLGVGFATRQVASMTKPTTIIEKNGDILTLKTHSTF KNTEISFKLGVEFDETTADDRKVKSIVTLDGGKLVHLQKWDGQETTLVRELIDGKLIL corresponding to amino acids 1-116 of FABH_HUMAN, which also corresponds to amino acids 1-116 of S67314_PEA_(—)1_P5 (SEQ ID NO: 652), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence DVLTAWPSIYRRQVKVLREDEITILPWHLQWSREKATKLLRPTLPSYNNHGWEELRVG KSIV (SEQ ID NO: 1081) corresponding to amino acids 117-178 of S67314_PEA_(—)1_P5 (SEQ ID NO: 652), wherein said first and second amino acid sequences are contiguous and in a sequential order.

According to preferred embodiments of the present invention, there is provided an isolated polypeptide encoding for a tail of S67314_PEA_(—)1_P5 (SEQ ID NO: 652), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence DVLTAWPSIYRRQVKVLREDEITILPWHLQWSREKATKLLRPTLPSYNNHGWEELRVG KSIV (SEQ ID NO: 1081) in S67314_PEA_(—)1_P5 (SEQ ID NO: 652).

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for S67314_PEA_(—)1_P5 (SEQ ID NO: 652), comprising a first amino acid sequence being at least 90% homologous to MVDAFLGTWKLVDSKNFDDYMKSLGVGFATRQVASMTKPTTIIEKNGDILTLKTHSTF KNTEISFKLGVEFDETTADDRKVKSIVTLDGGKLVHLQKWDGQETTLVRELIDGKLIL corresponding to amino acids 1-116 of AAP35373 (SEQ ID NO: 1007), which also corresponds to amino acids 1-116 of S67314_PEA_(—)1_P5 (SEQ ID NO: 652), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence DVLTAWPSIYRRQVKVLREDEITILPWHLQWSREKATKLLRPTLPSYNNHGWEELRVG KSIV (SEQ ID NO: 1081) corresponding to amino acids 117-178 of S67314_PEA_(—)1_P5 (SEQ ID NO: 652), wherein said first and second amino acid sequences are contiguous and in a sequential order.

According to preferred embodiments of the present invention, there is provided an isolated polypeptide encoding for a tail of S67314_PEA_(—)1_P5 (SEQ ID NO: 652), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence DVLTAWPSIYRRQVKVLREDEITILPWHLQWSREKATKLLRPTLPSYNNHGWEELRVG KSIV (SEQ ID NO: 1081) in S67314_PEA_(—)1_P5 (SEQ ID NO: 652).

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for S67314_PEA_(—)1_P6 (SEQ ID NO: 653), comprising a first amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence MVDAFLGTWKLVDSKNFDDYMKSLGVGFATRQVASMTKPTTIIEKNGDILTLKTHSTF KNTEISFKLGVEFDETTADDRKVKSIVTLDGGKLVHLQKWDGQETTLVRELIDGKLIL corresponding to amino acids 1-116 of FABH_HUMAN, which also corresponds to amino acids 1-116 of S67314_PEA_(—)1_P6 (SEQ ID NO: 653), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence MEKLQLRNVK (SEQ ID NO: 1082) corresponding to amino acids 117-126 of S67314_PEA_(—)1_P6 (SEQ ID NO: 653), wherein said first and second amino acid sequences are contiguous and in a sequential order.

According to preferred embodiments of the present invention, there is provided an isolated polypeptide encoding for a tail of S67314_PEA_(—)1_P6 (SEQ ID NO: 653), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence MEKLQLRNVK (SEQ ID NO: 1082) in S67314_PEA_(—)1_P6 (SEQ ID NO: 653).

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for S67314_PEA_(—)1_P6 (SEQ ID NO: 653), comprising a first amino acid sequence being at least 90% homologous to MVDAFLGTWKLVDSKNFDDYMKSLGVGFATRQVASMTKPTTIIEKNGDILTLKTHSTF KNTEISFKLGVEFDETTADDRKVKSIVTLDGGKLVHLQKWDGQETTLVRELIDGKLIL corresponding to amino acids 1-116 of AAP35373 (SEQ ID NO: 1007), which also corresponds to amino acids 1-116 of S67314_PEA_(—)1_P6 (SEQ ID NO: 653), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence MEKLQLRNVK (SEQ ID NO: 1082) corresponding to amino acids 117-126 of S67314_PEA_(—)1_P6 (SEQ ID NO: 653), wherein said first and second amino acid sequences are contiguous and in a sequential order.

According to preferred embodiments of the present invention, there is provided an isolated polypeptide encoding for a tail of S67314_PEA_(—)1_P6 (SEQ ID NO: 653), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence MEKLQLRNVK (SEQ ID NO: 1082) in S67314_PEA_(—)1_P6 (SEQ ID NO: 653).

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for S67314_PEA_(—)1_P7 (SEQ ID NO: 654), comprising a first amino acid sequence being at least 90% homologous to MVDAFLGTWKLVDSKNFDDYMKSL corresponding to amino acids 1-24 of FABH_HUMAN, which also corresponds to amino acids 1-24 of S67314_PEA_(—)1_P7 (SEQ ID NO: 654), second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence AHILITFPLPS (SEQ ID NO: 1143) corresponding to amino acids 25-35 of S67314_PEA_(—)1_P7 (SEQ ID NO: 654), and a third amino acid sequence being at least 90% homologous to GVGFATRQVASMTKPTTIIEKNGDILTLKTHSTFKNTEISFKLGVEFDETTADDRKVKSI VTLDGGKLVHLQKWDGQETTLVRELIDGKLILTLTHGTAVCTRTYEKEA corresponding to amino acids 25-133 of FABH_HUMAN, which also corresponds to amino acids 36-144 of S67314_PEA_(—)1_P7 (SEQ ID NO: 654), wherein said first, second, third and fourth amino acid sequences are contiguous and in a sequential order.

According to preferred embodiments of the present invention, there is provided an isolated polypeptide encoding for an edge portion of S67314_PEA_(—)1_P7 (SEQ ID NO: 654), comprising an amino acid sequence being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence encoding for AHILITFPLPS (SEQ ID NO: 1143), corresponding to S67314_PEA_(—)1_P7 (SEQ ID NO: 654).

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for S67314_PEA_(—)1_P7 (SEQ ID NO: 654), comprising a first amino acid sequence being at least 90% homologous to MVDAFLGTWKLVDSKNFDDYMKSL corresponding to amino acids 1-24 of AAP35373 (SEQ ID NO: 1007), which also corresponds to amino acids 1-24 of S67314_PEA_(—)1_P7 (SEQ ID NO: 654), second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence AHILITFPLPS (SEQ ID NO: 1143) corresponding to amino acids 25-35 of S67314_PEA_(—)1_P7 (SEQ ID NO: 654), and a third amino acid sequence being at least 90% homologous to GVGFATRQVASMTKPTTIIEKNGDILTLKTHSTFKNTEISFKLGVEFDETTADDRKVKSI VTLDGGKLVHLQKWDGQETTLVRELIDGKLILTLTHGTAVCTRTYEKEA corresponding to amino acids 25-133 of AAP35373 (SEQ ID NO: 1007), which also corresponds to amino acids 36-144 of S67314_PEA_(—)1_P7 (SEQ ID NO: 654), wherein said first, second and third amino acid sequences are contiguous and in a sequential order.

According to preferred embodiments of the present invention, there is provided an isolated polypeptide encoding for an edge portion of S67314_PEA_(—)1_P7 (SEQ ID NO: 654), comprising an amino acid sequence being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence encoding for AHILITFPLPS (SEQ ID NO: 1143), corresponding to S67314_PEA_(—)1_P7 (SEQ ID NO: 654).

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for Z39337_PEA_(—)2_PEA_(—)1_P4 (SEQ ID NO: 671), comprising a first amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence MWLPLSGAA (SEQ ID NO: 1083) corresponding to amino acids 1-9 of Z39337_PEA_(—)2_PEA_(—)1_P4 (SEQ ID NO: 671), and a second amino acid sequence being at least 90% homologous to MKKLMVVLSLIAAAWAEEQNKLVHGGPCDKTSHPYQAALYTSGHLLCGGVLIHPLWV LTAAHCKKPNLQVFLGKHNLRQRESSQEQSSVVRAVIHPDYDAASHDQDIMLLRLARP AKLSELIQPLPLERDCSANTTSCHILGWGKTADGDFPDTIQCAYIHLVSREECEHAYPGQ ITQNMLCAGDEKYGKDSCQGDSGGPLVCGDHLRGLVSWGNIPCGSKEKPGVYTNVCR YTNWIQKTIQAK corresponding to amino acids 1-244 of KLK6_HUMAN, which also corresponds to amino acids 10-253 of Z39337_PEA_(—)2_PEA_(—)1_P4 (SEQ ID NO: 671), wherein said first amino acid sequence and second amino acid sequence are contiguous and in a sequential order.

According to preferred embodiments of the present invention, there is provided an isolated polypeptide encoding for a head of Z39337_PEA_(—)2_PEA_(—)1_P4 (SEQ ID NO: 671), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence MWLPLSGAA (SEQ ID NO: 1083) of Z39337_PEA_(—)2_PEA_(—)1_P4 (SEQ ID NO: 671).

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for Z39337_PEA_(—)2_PEA_(—)1_P9 (SEQ ID NO: 672), comprising a first amino acid sequence being at least 90% homologous to MKKLMVVLSLIAAAWAEEQNKLVHGGPCDKTSHPYQAALYTSGHLLCGGVLIHPLWV LTAAHCKKPNLQVFLGKHNLRQRESSQEQSSVVRAVIHPDYDAASHDQDIMLLRLARP AKLSELIQPLPLERDCSANTTSCHILGWGKTADG corresponding to amino acids 1-149 of KLK6_HUMAN, which also corresponds to amino acids 1-149 of Z39337_PEA_(—)2_PEA_(—)1_P9 (SEQ ID NO: 672), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence Q corresponding to amino acids 150-150 of Z39337_PEA_(—)2_PEA_(—)1_P9 (SEQ ID NO: 672), wherein said first amino acid sequence and second amino acid sequence are contiguous and in a sequential order.

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for HUMPHOSLIP_PEA_(—)2_P10 (SEQ ID NO: 735), comprising a first amino acid sequence being at least 90% homologous to MALFGALFLALLAGAHAEFPGCKIRVTSKALELVKQEGLRFLEQELETITIPDLRGKEGH FYYNISE corresponding to amino acids 1-67 of PLTP_HUMAN, which also corresponds to amino acids 1-67 of HUMPHOSLIP_PEA_(—)2_P10 (SEQ ID NO: 735), and a second amino acid sequence being at least 90% homologous to KVYDFLSTFITSGMRFLLNQQICPVLYHAGTVLLNSLLDTVPVRSSVDELVGIDYSLMK DPVASTSNLDMDFRGAFFPLTERNWSLPNRAVEPQLQEEERMVYVAFSEFFFDSAMES YFRAGALQLLLVGDKVPHDLDMLLRATYFGSIVLLSPAVIDSPLKLELRVLAPPRCTIKP SGTTISVTASVTIALVPPDQPEVQLSSMTMDARLSAKMALRGKALRTQLDLRRFRIYSN HSALESLALIPLQAPLKTMLQIGVMPMLNERTWRGVQIPLPEGINFVHEVVTNHAGFLTI GADLHFAKGLREVIEKNRPADVRASTAPTPSTAAV corresponding to amino acids 163-493 of PLTP_HUMAN, which also corresponds to amino acids 68-398 of HUMPHOSLIP_PEA_(—)2_P10 (SEQ ID NO: 735), wherein said first amino acid sequence and second amino acid sequence are contiguous and in a sequential order.

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for an edge portion of HUMPHOSLIP_PEA_(—)2_P10 (SEQ ID NO: 735), comprising a polypeptide having a length “n”, wherein n is at least about 10 amino acids in length, optionally at least about 20 amino acids in length, preferably at least about 30 amino acids in length, more preferably at least about 40 amino acids in length and most preferably at least about 50 amino acids in length, wherein at least two amino acids comprise EK, having a structure as follows: a sequence starting from any of amino acid numbers 67-x to 67; and ending at any of amino acid numbers 68+((n−2)−x), in which x varies from 0 to n−2.

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for HUMPHOSLIP_PEA_(—)2_P12 (SEQ ID NO: 736), comprising a first amino acid sequence being at least 90% homologous to MALFGALFLALLAGAHAEFPGCKIRVTSKALELVKQEGLRFLEQELETITIPDLRGKEGH FYYNISEVKVTELQLTSSELDFQPQQELMLQITNASLGLRFRRQLLYWFFYDGGYINAS AEGVSIRTGLELSRDPAGRMKVSNVSCQASVSRMHAAFGGTFKKVYDFLSTFITSGMRF LLNQQICPVLYHAGTVLLNSLLDTVPVRSSVDELVGIDYSLMKDPVASTSNLDMDFRG AFFPLTERNWSLPNRAVEPQLQEEERMVYVAFSEFFFDSAMESYFRAGALQLLLVGDK VPHDLDMLLRATYFGSIVLLSPAVIDSPLKLELRVLAPPRCTIKPSGTTISVTASVTIALVP PDQPEVQLSSMTMDARLSAKMALRGKALRTQLDLRRFRIYSNHSALESLALIPLQAPLK TMLQIGVMPMLN corresponding to amino acids 1-427 of PLTP_HUMAN, which also corresponds to amino acids 1-427 of HUMPHOSLIP_PEA_(—)2_P12 (SEQ ID NO: 736), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence GKAGV (SEQ ID NO: 1084) corresponding to amino acids 428-432 of HUMPHOSLIP_PEA_(—)2_P12 (SEQ ID NO: 736), wherein said first amino acid sequence and second amino acid sequence are contiguous and in a sequential order.

According to preferred embodiments of the present invention, there is provided an isolated polypeptide encoding for a tail of HUMPHOSLIP_PEA_(—)2_P12 (SEQ ID NO: 736), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence GKAGV (SEQ ID NO: 1084) in HUMPHOSLIP_PEA_(—)2_P12 (SEQ ID NO: 736).

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for HUMPHOSLIP_PEA_(—)2_P31 (SEQ ID NO: 738), comprising a first amino acid sequence being at least 90% homologous to MALFGALFLALLAGAHAEFPGCKIRVTSKALELVKQEGLRFLEQELETITIPDLRGKEGH FYYNISE corresponding to amino acids 1-67 of PLTP_HUMAN, which also corresponds to amino acids 1-67 of HUMPHOSLIP_PEA_(—)2_P31 (SEQ ID NO: 738), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence PGLERGADKFPVVGGSSLFLALDLTLRPPVG (SEQ ID NO: 1085) corresponding to amino acids 68-98 of HUMPHOSLIP_PEA_(—)2_P31 (SEQ ID NO: 738), wherein said first amino acid sequence and second amino acid sequence are contiguous and in a sequential order.

According to preferred embodiments of the present invention, there is provided an isolated polypeptide encoding for a tail of HUMPHOSLIP_PEA_(—)2_P31 (SEQ ID NO: 738), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence PGLERGADKFPVVGGSSLFLALDLTLRPPVG (SEQ ID NO: 1085) in HUMPHOSLIP_PEA_(—)2_P31 (SEQ ID NO: 738).

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for HUMPHOSLIP_PEA_(—)2_P33 (SEQ ID NO: 739), comprising a first amino acid sequence being at least 90% homologous to MALFGALFLALLAGAHAEFPGCKIRVTSKALELVKQEGLRFLEQELETITIPDLRGKEGH FYYNISEVKVTELQLTSSELDFQPQQELMLQITNASLGLRFRRQLLYWFFYDGGYINAS AEGVSIRTGLELSRDPAGRMKVSNVSCQASVSRMHAAFGGTFKKVYDFLSTFITSGMRF LLNQQ corresponding to amino acids 1-183 of PLTP_HUMAN, which also corresponds to amino acids 1-183 of HUMPHOSLIP_PEA_(—)2_P33 (SEQ ID NO: 739), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence VWAATGRRVARVGMLSL (SEQ ID NO: 1086) corresponding to amino acids 184-200 of HUMPHOSLIP_PEA_(—)2_P33 (SEQ ID NO: 739), wherein said first amino acid sequence and second amino acid sequence are contiguous and in a sequential order.

According to preferred embodiments of the present invention, there is provided an isolated polypeptide encoding for a tail of HUMPHOSLIP_PEA_(—)2_P33 (SEQ ID NO: 739), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence VWAATGRRVARVGMLSL (SEQ ID NO: 1086) in HUMPHOSLIP_PEA_(—)2_P33 (SEQ ID NO: 739).

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for HUMPHOSLIP_PEA_(—)2_P34 (SEQ ID NO: 740), comprising a first amino acid sequence being at least 90% homologous to MALFGALFLALLAGAHAEFPGCKIRVTSKALELVKQEGLRFLEQELETITIPDLRGKEGH FYYNISEVKVTELQLTSSELDFQPQQELMLQITNASLGLRFRRQLLYWFFYDGGYINAS AEGVSIRTGLELSRDPAGRMKVSNVSCQASVSRMHAAFGGTFKKVYDFLSTFITSGMRF LLNQQICPVLYHAGTVLLNSLLDTVPV corresponding to amino acids 1-205 of PLTP_HUMAN, which also corresponds to amino acids 1-205 of HUMPHOSLIP_PEA_(—)2_P34 (SEQ ID NO: 740), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence LWTSLLALTIPS (SEQ ID NO: 1087) corresponding to amino acids 206-217 of HUMPHOSLIP_PEA_(—)2_P34 (SEQ ID NO: 740), wherein said first amino acid sequence and second amino acid sequence are contiguous and in a sequential order.

According to preferred embodiments of the present invention, there is provided an isolated polypeptide encoding for a tail of HUMPHOSLIP_PEA_(—)2_P34 (SEQ ID NO: 740), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence LWTSLLALTIPS (SEQ ID NO: 1087) in HUMPHOSLIP_PEA_(—)2_P34 (SEQ ID NO: 740).

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for HUMPHOSLIP_PEA_(—)2_P35 (SEQ ID NO: 741), comprising a first amino acid sequence being at least 90% homologous to MALFGALFLALLAGAHAEFPGCKIRVTSKALELVKQEGLRFLEQELETITIPDLRGKEGH FYYNISEVKVTELQLTSSELDFQPQQELMLQITNASLGLRFRRQLLYWF corresponding to amino acids 1-109 of PLTP_HUMAN, which also corresponds to amino acids 1-109 of HUMPHOSLIP_PEA_(—)2_P35 (SEQ ID NO: 741), a second amino acid sequence bridging amino acid sequence comprising of L, a third amino acid sequence being at least 90% homologous to KVYDFLSTFITSGMRFLLNQQ corresponding to amino acids 163-183 of PLTP_HUMAN, which also corresponds to amino acids 111-131 of HUMPHOSLIP_PEA_(—)2_P35 (SEQ ID NO: 741), and a fourth amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence VWAATGRRVARVGMLSL (SEQ ID NO: 1086) corresponding to amino acids 132-148 of HUMPHOSLIP_PEA_(—)2_P35 (SEQ ID NO: 741), wherein said first amino acid sequence, second amino acid sequence, third amino acid sequence and fourth amino acid sequence are contiguous and in a sequential order.

According to preferred embodiments of the present invention, there is provided an isolated polypeptide encoding for an edge portion of HUMPHOSLIP_PEA_(—)2_P35 (SEQ ID NO: 741), comprising a polypeptide having a length “n”, wherein n is at least about 10 amino acids in length, optionally at least about 20 amino acids in length, preferably at least about 30 amino acids in length, more preferably at least about 40 amino acids in length and most preferably at least about 50 amino acids in length, wherein at least two amino acids comprise FLK having a structure as follows (numbering according to HUMPHOSLIP_PEA_(—)2_P35 (SEQ ID NO: 741)): a sequence starting from any of amino acid numbers 109-x to 109; and ending at any of amino acid numbers 111+((n−2)−x), in which x varies from 0 to n−2.

According to preferred embodiments of the present invention, there is provided an isolated polypeptide encoding for a tail of HUMPHOSLIP_PEA_(—)2_P35 (SEQ ID NO: 741), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence VWAATGRRVARVGMLSL (SEQ ID NO: 1086) in HUMPHOSLIP_PEA_(—)2_P35 (SEQ ID NO: 741).

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for T59832_P7 (SEQ ID NO: 779), comprising a first amino acid sequence being at least 90% homologous to MTLSPLLLFLPPLLLLLDVPTAAVQASPLQALDFFGNGPPVNYKTGNLYLRGPLKKSNA PLVNVTLYYEALCGGCRAFLIRELFPTWLLVMEILNVTLVPYGNAQEQNVSGRWEFKC QHGEEECKFNKVEACVLDELDMELAFLTIVCMEEFEDMERSLPLCLQLYAPGLSPDTIM ECAMGDRGMQLMHANAQRTDALQPPHEYVPWVTVNG corresponding to amino acids 12-223 of GILT_HUMAN, which also corresponds to amino acids 1-212 of T59832_P7 (SEQ ID NO: 779), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence VRIFLALSLTLIVPWSQGWTRQRDQR (SEQ ID NO: 1089) corresponding to amino acids 213-238 of T59832_P7 (SEQ ID NO: 779), wherein said first amino acid sequence and second amino acid sequence are contiguous and in a sequential order.

According to preferred embodiments of the present invention, there is provided an isolated polypeptide encoding for a tail of T59832_P7 (SEQ ID NO: 779), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence VRIFLALSLTLIVPWSQGWTRQRDQR (SEQ ID NO: 1089) in T59832_P7 (SEQ ID NO: 779).

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for T59832_P9 (SEQ ID NO: 780), comprising a first amino acid sequence being at least 90% homologous to MTLSPLLLFLPPLLLLLDVPTAAVQASPLQALDFFGNGPPVNYKTGNLYLRGPLKKSNA PLVNVTLYYEALCGGCRAFLIRELFPTWLLVMEILNVTLVPYGNAQEQNVSGRWEFKC QHGEEECKFNKVEACVLDELDMELAFLTIVCMEEFEDMERSLPLCLQLYAPGLSPDTIM ECAMGDRGMQLMHANAQRTDALQPPHE corresponding to amino acids 12-214 of GILT_HUMAN, which also corresponds to amino acids 1-203 of T59832_P9 (SEQ ID NO: 780), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence NPWKIRPSSLPLSASCTRARSRMSALPQPAPSGVFASSDGR (SEQ ID NO: 1090) corresponding to amino acids 204-244 of T59832_P9 (SEQ ID NO: 780), wherein said first amino acid sequence and second amino acid sequence are contiguous and in a sequential order.

According to preferred embodiments of the present invention, there is provided an isolated polypeptide encoding for a tail of T59832_P9 (SEQ ID NO: 780), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence NPWKIRPSSLPLSASCTRARSRMSALPQPAPSGVFASSDGR (SEQ ID NO: 1090) in T59832_P9 (SEQ ID NO: 780).

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for T59832_P12 (SEQ ID NO: 781), comprising a first amino acid sequence being at least 90% homologous to MTLSPLLLFLPPLLLLLDVPTAAVQASPLQALDFFGNGPPVNYKTGNLYLRGPLKKSNA PLVNVTLYYEALCGGCRAFLIRELFPTWLLVMEILNVTLVPYGNAQEQNVSGRWEFKC QHGEEECKFNKVE corresponding to amino acids 12-141 of GILT_HUMAN, which also corresponds to amino acids 1-130 of T59832_P12 (SEQ ID NO: 781), and a second amino acid sequence being at least 90% homologous to CLQLYAPGLSPDTIMECAMGDRGMQLMHANAQRTDALQPPHEYVPWVTVNGKPLED QTQLLTLVCQLYQGKKPDVCPSSTSSLRSVCFK corresponding to amino acids 173-261 of GILT_HUMAN, which also corresponds to amino acids 131-219 of T59832_P12 (SEQ ID NO: 781), wherein said first amino acid sequence and second amino acid sequence are contiguous and in a sequential order.

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for an edge portion of T59832_P12 (SEQ ID NO: 781), comprising a polypeptide having a length “n”, wherein n is at least about 10 amino acids in length, optionally at least about 20 amino acids in length, preferably at least about 30 amino acids in length, more preferably at least about 40 amino acids in length and most preferably at least about 50 amino acids in length, wherein at least two amino acids comprise EC, having a structure as follows: a sequence starting from any of amino acid numbers 130-x to 130; and ending at any of amino acid numbers 131+((n−2)−x), in which x varies from 0 to n−2.

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for T59832_P18 (SEQ ID NO: 782), comprising a first amino acid sequence being at least 90% homologous to MTLSPLLLFLPPLLLLLDVPTAAVQASPLQALDFFGNGPPVNYK corresponding to amino acids 12-55 of GILT_HUMAN, which also corresponds to amino acids 1-44 of T59832_P18 (SEQ ID NO: 782), and a second amino acid sequence being at least 90% homologous to CLQLYAPGLSPDTIMECAMGDRGMQLMHANAQRTDALQPPHEYVPWVTVNGKPLED QTQLLTLVCQLYQGKKPDVCPSSTSSLRSVCFK corresponding to amino acids 173-261 of GILT_HUMAN, which also corresponds to amino acids 45-133 of T59832_P18 (SEQ ID NO: 782), wherein said first amino acid sequence and second amino acid sequence are contiguous and in a sequential order.

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for an edge portion of T59832_P18 (SEQ ID NO: 782), comprising a polypeptide having a length “n”, wherein n is at least about 10 amino acids in length, optionally at least about 20 amino acids in length, preferably at least about 30 amino acids in length, more preferably at least about 40 amino acids in length and most preferably at least about 50 amino acids in length, wherein at least two amino acids comprise KC, having a structure as follows: a sequence starting from any of amino acid numbers 44-x to 44; and ending at any of amino acid numbers 45+((n−2)−x), in which x varies from 0 to n−2.

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for HSCP2_PEA_(—)1_P4 (SEQ ID NO: 846), comprising a first amino acid sequence being at least 90% homologous to MKILILGIFLFLCSTPAWAKEKHYYIGIIETTWDYASDHGEKKLISVDTEHSNIYLQNGPD RIGRLYKKALYLQYTDETFRTTIEKPVWLGFLGPIIKAETGDKVYVHLKNLASRPYTFHS HGITYYKEHEGAIYPDNTTDFQRADDKVYPGEQYTYMLLATEEQSPGEGDGNCVTRIY HSHIDAPKDIASGLIGPLIICKKDSLDKEKEKHIDREFVVMFSVVDENFSWYLEDNIKTY CSEPEKVDKDNEDFQESNRMYSVNGYTFGSLPGLSMCAEDRVKWYLFGMGNEVDVH AAFFHGQALTNKNYRIDTINLFPATLFDAYMVAQNPGEWMLSCQNLNHLKAGLQAFF QVQECNKSSSKDNIRGKHVRHYYIAAEEIIWNYAPSGIDIFTKENLTAPGSDSAVFFEQG TTRIGGSYKKLVYREYTDASFTNRKERGPEEEHLGILGPVIWAEVGDTIRVTFHNKGAY PLSIEPIGVRFNKNNEGTYYSPNYNPQSRSVPPSASHVAPTETFTYEWTVPKEVGPTNAD PVCLAKMYYSAVDPTKDIFTGLIGPMKICKKGSLHANGRQKDVDKEFYLFPTVFDENES LLLEDNIRMFTTAPDQVDKEDEDFQESNKMHSMNGFMYGNQPGLTMCKGDSVVWYL FSAGNEADVHGIYFSGNTYLWRGERRDTANLFPQTSLTLHMWPDTEGTFNVECLTTDH YTGGMKQKYTVNQCRRQSEDSTFYLGERTYYIAAVEVEWDYSPQREWEKELHHLQEQ NVSNAFLDKGEFYIGSKYKKVVYRQYTDSTFRVPVERKAEEEHLGILGPQLHADVGDK VKIIFKNMATRPYSIHAHGVQTESSTVTPTLPGETLTYVWKIPERSGAGTEDSACIPWAY YSTVDQVKDLYSGLIGPLIVCRRPYLKVFNPRRKLEFALLFLVFDENESWYLDDNIKTYS DHPEKVNKDDEEFIESNKMHAINGRMFGNLQGLTMHVGDEVNWYLMGMGNEIDLHT VHFHGHSFQYKHRGVYSSDVFDIFPGTYQTLEMFPRTPGIWLLHCHVTDHIHAGMETT YTVLQNE corresponding to amino acids 1-1060 of CERU_HUMAN, which also corresponds to amino acids 1-1060 of HSCP2_PEA_(—)1_P4 (SEQ ID NO: 846), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence GGTSM (SEQ ID NO: 1091) corresponding to amino acids 1061-1065 of HSCP2_PEA_(—)1_P4 (SEQ ID NO: 846), wherein said first amino acid sequence and second amino acid sequence are contiguous and in a sequential order.

According to preferred embodiments of the present invention, there is provided an isolated polypeptide encoding for a tail of HSCP2_PEA_(—)1_P4 (SEQ ID NO: 846), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence GGTSM (SEQ ID NO: 1091) in HSCP2_PEA_(—)1_P4 (SEQ ID NO: 846).

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for HSCP2_PEA_(—)1_P8 (SEQ ID NO: 847), comprising a first amino acid sequence being at least 90% homologous to MKILILGIFLFLCSTPAWAKEKHYYIGIIETTWDYASDHGEKKLISVDTEHSNIYLQNGPD RIGRLYKKALYLQYTDETFRTTIEKPVWLGFLGPIIKAETGDKVYVHLKNLASRPYTFHS HGITYYKEHEGAIYPDNTTDFQRADDKVYPGEQYTYMLLATEEQSPGEGDGNCVTRIY HSHIDAPKDIASGLIGPLIICKKDSLDKEKEKHIDREFVVMFSVVDENFSWYLEDNIKTY CSEPEKVDKDNEDFQESNRMYSVNGYTFGSLPGLSMCAEDRVKWYLFGMGNEVDVH AAFFHGQALTNKNYRIDTINLFPATLFDAYMVAQNPGEWMLSCQNLNHLKAGLQAFF QVQECNKSSSKDNIRGKHVRHYYIAAEEIIWNYAPSGIDIFTKENLTAPGSDSAVFFEQG TTRIGGSYKKLVYREYTDASFTNRKERGPEEEHLGILGPVIWAEVGDTIRVTFHNKGAY PLSIEPIGVRFNKNNEGTYYSPNYNPQSRSVPPSASHVAPTETFTYEWTVPKEVGPTNAD PVCLAKMYYSAVDPTKDIFTGLIGPMKICKKGSLHANGRQKDVDKEFYLFPTVFDENES LLLEDNIRMFTTAPDQVDKEDEDFQESNKMHSMNGFMYGNQPGLTMCKGDSVVWYL FSAGNEADVHGIYFSGNTYLWRGERRDTANLFPQTSLTLHMWPDTEGTFNVECLTTDH YTGGMKQKYTVNQCRRQSEDSTFYLGERTYYIAAVEVEWDYSPQREWEKELHHLQEQ NVSNAFLDKGEFYIGSKYKKVVYRQYTDSTFRVPVERKAEEEHLGILGPQLHADVGDK VKIIFKNMATRPYSIHAHGVQTESSTVTPTLPGETLTYVWKIPERSGAGTEDSACIPWAY YSTVDQVKDLYSGLIGPLIVCRRPYLKVFNPRRKLEFALLFLVFDENESWYLDDNIKTYS DHPEKVNKDDEEFIESNKMHAINGRMFGNLQGLTMHVGDEVNWYLMGMGNEIDLHT VHFHGHSFQYK corresponding to amino acids 1-1006 of CERU_HUMAN, which also corresponds to amino acids 1-1006 of HSCP2_PEA_(—)1_P8 (SEQ ID NO: 847), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence KCFQEHLEFGYSTAM (SEQ ID NO: 1092) corresponding to amino acids 1007-1021 of HSCP2_PEA_(—)1_P8 (SEQ ID NO: 847), wherein said first amino acid sequence and second amino acid sequence are contiguous and in a sequential order.

According to preferred embodiments of the present invention, there is provided an isolated polypeptide encoding for a tail of HSCP2_PEA_(—)1_P8 (SEQ ID NO: 847), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence KCFQEHLEFGYSTAM (SEQ ID NO: 1092) in HSCP2_PEA_(—)1_P8 (SEQ ID NO: 847).

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for HSCP2_PEA_(—)1_P14 (SEQ ID NO: 848), comprising a first amino acid sequence being at least 90% homologous to MKILILGIFLFLCSTPAWAKEKHYYIGIIETTWDYASDHGEKKLISVDTEHSNIYLQNGPD RIGRLYKKALYLQYTDETFRTTIEKPVWLGFLGPIIKAETGDKVYVHLKNLASRPYTFHS HGITYYKEHEGAIYPDNTTDFQRADDKVYPGEQYTYMLLATEEQSPGEGDGNCVTRIY HSHIDAPKDIASGLIGPLIICKKDSLDKEKEKHIDREFVVMFSVVDENFSWYLEDNIKTY CSEPEKVDKDNEDFQESNRMYSVNGYTFGSLPGLSMCAEDRVKWYLFGMGNEVDVH AAFFHGQALTNKNYRIDTINLFPATLFDAYMVAQNPGEWMLSCQNLNHLKAGLQAFF QVQECNKSSSKDNIRGKHVRHYYIAAEEIIWNYAPSGIDIFTKENLTAPGSDSAVFFEQG TTRIGGSYKKLVYREYTDASFTNRKERGPEEEHLGILGPVIWAEVGDTIRVTFHNKGAY PLSIEPIGVRFNKNNEGTYYSPNYNPQSRSVPPSASHVAPTETFTYEWTVPKEVGPTNAD PVCLAKMYYSAVDPTKDIFTGLIGPMKICKKGSLHANGRQKDVDKEFYLFPTVFDENES LLLEDNIRMFTTAPDQVDKEDEDFQESNKMH corresponding to amino acids 1-621 of CERU_HUMAN, which also corresponds to amino acids 1-621 of HSCP2_PEA_(—)1_P14 (SEQ ID NO: 848), a second amino acid sequence bridging amino acid sequence comprising of W, and a third amino acid sequence being at least 90% homologous to TFNVECLTTDHYTGGMKQKYTVNQCRRQSEDSTFYLGERTYYIAAVEVEWDYSPQRE WEKELHHLQEQNVSNAFLDKGEFYIGSKYKKVVYRQYTDSTFRVPVERKAEEEHLGIL GPQLHADVGDKVKIIFKNMATRPYSIHAHGVQTESSTVTPTLPGETLTYVWKIPERSGA GTEDSACIPWAYYSTVDQVKDLYSGLIGPLIVCRRPYLKVFNPRRKLEFALLFLVFDENE SWYLDDNIKTYSDHPEKVNKDDEEFIESNKMHAINGRMFGNLQGLTMHVGDEVNWYL MGMGNEIDLHTVHFHGHSFQYKHRGVYSSDVFDIFPGTYQTLEMFPRTPGIWLLHCHV TDHIHAGMETTYTVLQNEDTKSG corresponding to amino acids 694-1065 of CERU_HUMAN, which also corresponds to amino acids 623-994 of HSCP2_PEA_(—)1_P14 (SEQ ID NO: 848), wherein said first amino acid sequence, second amino acid sequence and third amino acid sequence are contiguous and in a sequential order.

According to preferred embodiments of the present invention, there is provided an isolated polypeptide encoding for an edge portion of HSCP2_PEA_(—)1_P14 (SEQ ID NO: 848), comprising a polypeptide having a length “n”, wherein n is at least about 10 amino acids in length, optionally at least about 20 amino acids in length, preferably at least about 30 amino acids in length, more preferably at least about 40 amino acids in length and most preferably at least about 50 amino acids in length, wherein at least two amino acids comprise HWT having a structure as follows (numbering according to HSCP2_PEA_(—)1_P14 (SEQ ID NO: 848)): a sequence starting from any of amino acid numbers 621-x to 621; and ending at any of amino acid numbers 623+((n−2)−x), in which x varies from 0 to n−2.

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for HSCP2_PEA_(—)1_P15 (SEQ ID NO: 849), comprising a first amino acid sequence being at least 90% homologous to MKILILGIFLFLCSTPAWAKEKHYYIGIIETTWDYASDHGEKKLISVDTEHSNIYLQNGPD RIGRLYKKALYLQYTDETFRTTIEKPVWLGFLGPIIKAETGDKVYVHLKNLASRPYTFHS HGITYYKEHEGAIYPDNTTDFQRADDKVYPGEQYTYMLLATEEQSPGEGDGNCVTRIY HSHIDAPKDIASGLIGPLIICKKDSLDKEKEKHIDREFVVMFSVVDENFSWYLEDNIKTY CSEPEKVDKDNEDFQESNRMYSVNGYTFGSLPGLSMCAEDRVKWYLFGMGNEVDVH AAFFHGQALTNKNYRIDTINLFPATLFDAYMVAQNPGEWMLSCQNLNHLKAGLQAFF QVQECNKSSSKDNIRGKHVRHYYIAAEEIIWNYAPSGIDIFTKENLTAPGSDSAVFFEQG TTRIGGSYKKLVYREYTDASFTNRKERGPEEEHLGILGPVIWAEVGDTIRVTFHNKGAY PLSIEPIGVRFNKNNEGTYYSPNYNPQSRSVPPSASHVAPTETFTYEWTVPKEVGPTNAD PVCLAKMYYSAVDPTKDIFTGLIGPMKICKKGSLHANGRQKDVDKEFYLFPTVFDENES LLLEDNIRMFTTAPDQVDKEDEDFQESNKMHSMNGFMYGNQPGLTMCKGDSVVWYL FSAGNEADVHGIYFSGNTYLWRGERRDTANLFPQTSLTLHMWPDTEGTFNVECLTTDH YTGGMKQKYTVNQCRRQSEDSTFYLGERTYYIAAVEVEWDYSPQREWEKELHHLQEQ NVSNAFLDKGEFYIGSKYKKVVYRQYTDSTFRVPVERKAEEEHLGILGPQLHADVGDK VKIIFKNMATRPYSIHAHGVQTESSTVTPTLPGETLTYVWKIPERSGAGTEDSACIPWAY YSTVDQVKDLYSGLIGPLIVCRRPYLKVFNPRRKLEFALLFLVFDENESWYLDDNIKTYS DHPEKVNKDDEEFIESNKMHAINGRMFGNLQGLTMHVGDEVNWYLMGMGNEIDLHT VHFHGHSFQYKHRGVYSSDVFDIFPGTYQTLEMFPRTPGIWLLHCHVTDHIHAGMETT YTVLQNE corresponding to amino acids 1-1060 of CERU_HUMAN, which also corresponds to amino acids 1-1060 of HSCP2_PEA_(—)1_P15 (SEQ ID NO: 849), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence GEYPASSETHRRIWNVIYPITVSVIILFQISTKE (SEQ ID NO: 1093) corresponding to amino acids 1061-1094 of HSCP2_PEA_(—)1_P15 (SEQ ID NO: 849), wherein said first amino acid sequence and second amino acid sequence are contiguous and in a sequential order.

According to preferred embodiments of the present invention, there is provided an isolated polypeptide encoding for a tail of HSCP2_PEA_(—)1_P15 (SEQ ID NO: 849), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence GEYPASSETHRRIWNVIYPITVSVIILFQISTKE (SEQ ID NO: 1093) in HSCP2_PEA_(—)1_P15 (SEQ ID NO: 849).

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for HSCP2_PEA_(—)1_P2 (SEQ ID NO: 850), comprising a first amino acid sequence being at least 90% homologous to MKILILGIFLFLCSTPAWAKEKHYYIGIIETTWDYASDHGEKKLISVDTEHSNIYLQNGPD RIGRLYKKALYLQYTDETFRTTIEKPVWLGFLGPIIKAETGDKVYVHLKNLASRPYTFHS HGITYYKEHEGAIYPDNTTDFQRADDKVYPGEQYTYMLLATEEQSPGEGDGNCVTRIY HSHIDAPKDIASGLIGPLIICKKDSLDKEKEKHIDREFVVMFSVVDENFSWYLEDNIKTY CSEPEKVDKDNEDFQESNRMYSVNGYTFGSLPGLSMCAEDRVKWYLFGMGNEVDVH AAFFHGQALTNKNYRIDTINLFPATLFDAYMVAQNPGEWMLSCQNLNHLKAGLQAFF QVQECNKSSSKDNIRGKHVRHYYIAAEEIIWNYAPSGIDIFTKENLTAPGSDSAVFFEQG TTRIGGSYKKLVYREYTDASFTNRKERGPEEEHLGILGPVIWAEVGDTIRVTFHNKGAY PLSIEPIGVRFNKNNEGTYYSPNYNPQSRSVPPSASHVAPTETFTYEWTVPKEVGPTNAD PVCLAKMYYSAVDPTKDIFTGLIGPMKICKKGSLHANGRQKDVDKEFYLFPTVFDENES LLLEDNIRMFTTAPDQVDKEDEDFQESNKMHSMNGFMYGNQPGLTMCKGDSVVWYL FSAGNEADVHGIYFSGNTYLWRGERRDTANLFPQTSLTLHMWPDTEGTFNVECLTTDH YTGGMKQKYTVNQCRRQSEDSTFYLGERTYYIAAVEVEWDYSPQREWEKELHHLQEQ corresponding to amino acids 1-761 of CERU_HUMAN, which also corresponds to amino acids 1-761 of HSCP2_PEA_(—)1_P2 (SEQ ID NO: 850), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence K corresponding to amino acids 762-762 of HSCP2_PEA_(—)1_P2 (SEQ ID NO: 850), wherein said first amino acid sequence and second amino acid sequence are contiguous and in a sequential order.

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for HSCP2_PEA_(—)1_P16 (SEQ ID NO: 851), comprising a first amino acid sequence being at least 90% homologous to MKILILGIFLFLCSTPAWAKEKHYYIGIIETTWDYASDHGEKKILISVDTEHSNIYLQNGPD RIGRLYKKALYLQYTDETFRTTIEKPVWLGFLGPIIKAETGDKVYVHLKNLASRPYTFHS HGITYYKEHEGAIYPDNTTDFQRADDKVYPGEQYTYMLLATEEQSPGEGDGNCVTRIY HSHIDAPKDIASGLIGPLIICKKDSLDKEKEKHIDREFVVMFSVVDENFSWYLEDNIKTY CSEPEKVDKDNEDFQESNRMYSVNGYTFGSLPGLSMCAEDRVKWYLFGMGNEVDVH AAFFHGQALTNKNYRIDTINLFPATLFDAYMVAQNPGEWMLSCQNLNHLKAGLQAFF QVQECNKSSSKDNIRGKHVRHYYIAAEEIIWNYAPSGIDIFTKENLTAPGSDSAVFFEQG TTRIGGSYKKLVYREYTDASFTNRKERGPEEEHLGILGPVIWAEVGDTIRVTFHNKGAY PLSIEPIGVRFNKNNEGTYYSPNYNPQSRSVPPSASHVAPTETFTYEWTVPKEVGPTNAD PVCLAKMYYSAVDPTKDIFTGLIGPMKICKKGSLHANGRQKDVDKEFYLFPTVFDENES LLLEDNIRMFTTAPDQVDKEDEDFQESNKMHSMNGFMYGNQPGLTMCKGDSVVWYL FSAGNEADVHGIYFSGNTYLWRGERRDTANLFPQTSLTLHMWPDTEGTFNVECLTTDH YTGGMKQKYTVNQCRRQSEDSTFYLGERTYYIAAVEVEWDYSPQREWEKELHHLQEQ NVSNAFLDKGEFYIGSKYKKVVYRQYTDSTFRVPVERKAEEEHLGILGPQLHADVGDK VKIIFKNMATRPYSIHAHGVQTESSTVTPTLPGETLTYVWKIPERSGAGTEDSACIPWAY YSTVDQVKDLYSGLIGPLIVCRRPYLKVFNPRRKLEFALLFLVFDENESWYLDDNIKTYS DHPEKVNKDDEEFIESNKMHAINGRMFGNLQGLTMHVGDEVNWYLMGMGNEIDLHT VHFHGHSFQYKH corresponding to amino acids 1-1007 of CERU_HUMAN, which also corresponds to amino acids 1-1007 of HSCP2_PEA_(—)1_P16 (SEQ ID NO: 851), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence LLRLTGEYGM (SEQ ID NO: 1094) corresponding to amino acids 1008-1017 of HSCP2_PEA_(—)1_P16 (SEQ ID NO: 851), wherein said first amino acid sequence and second amino acid sequence are contiguous and in a sequential order.

According to preferred embodiments of the present invention, there is provided an isolated polypeptide encoding for a tail of HSCP2_PEA_(—)1_P16 (SEQ ID NO: 851), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence LLRLTGEYGM (SEQ ID NO: 1094) in HSCP2_PEA_(—)1_P16 (SEQ ID NO: 851).

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for HSCP2_PEA_(—)1_P6 (SEQ ID NO: 852), comprising a first amino acid sequence being at least 90% homologous to MKILILGIFLFLCSTPAWAKEKHYYIGIIETTWDYASDHGEKKLISVDTEHSNIYLQNGPD RIGRLYKKALYLQYTDETFRTTIEKPVWLGFLGPIIKAETGDKVYVHLKNLASRPYTFHS HGITYYKEHEGAIYPDNTTDFQRADDKVYPGEQYTYMLLATEEQSPGEGDGNCVTRIY HSHIDAPKDIASGLIGPLIICKKDSLDKEKEKHIDREFVVMFSVVDENFSWYLEDNIKTY CSEPEKVDKDNEDFQESNRMYSVNGYTFGSLPGLSMCAEDRVKWYLFGMGNEVDVH AAFFHGQALTNKNYRIDTINLFPATLFDAYMVAQNPGEWMLSCQNLNHLKAGLQAFF QVQECNKSSSKDNIRGKHVRHYYIAAEEIIWNYAPSGIDIFTKENLTAPGSDSAVFFEQG TTRIGGSYKKLVYREYTDASFTNRKERGPEEEHLGILGPVIWAEVGDTIRVTFHNKGAY PLSIEPIGVRFNKNNEGTYYSPNYNPQSRSVPPSASHVAPTETFTYEWTVPKEVGPTNAD PVCLAKMYYSAVDPTKDIFTGLIGPMKICKKGSLHANGRQKDVDKEFYLFPTVFDENES LLLEDNIRMFTTAPDQVDKEDEDFQESNKMHSMNGFMYGNQPGLTMCKGDSVVWYL FSAGNEADVHGIYFSGNTYLWRGERRDTANLFPQTSLTLHMWPDTEGTFNVECLTTDH YTGGMKQKYTVNQCRRQSEDSTFYLGERTYYIAAVEVEWDYSPQREWEKELHHLQEQ NVSNAFLDKGEFYIGSKYKKVVYRQYTDSTFRVPVERKAEEEHLGILGPQLHADVGDK VKIIFKNMATRPYSIHAHGVQTESSTVTPTLPGETLTYVWKIPERSGAGTEDSACIPWAY YSTVDQVKDLYSGLIGPLIVCRRPYLKVFNPRRKLEFALLFLVFDENESWYLDDNIKTYS DHPEKVNKDDEEFIESNKMHAINGRMFGNLQGLTMHVGDEVNWYLMGMGNEIDLHT VHFHGHSFQYK corresponding to amino acids 1-1006 of CERU_HUMAN, which also corresponds to amino acids 1-1006 of HSCP2_PEA_(—)1_P6 (SEQ ID NO: 852), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence GSL corresponding to amino acids 1007-1009 of HSCP2_PEA_(—)1_P6 (SEQ ID NO: 852), wherein said first amino acid sequence and second amino acid sequence are contiguous and in a sequential order.

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for HSCP2_PEA_(—)1_P22 (SEQ ID NO: 853), comprising a first amino acid sequence being at least 90% homologous to MKILILGIFLFLCSTPAWAKEKHYYIGIIETTWDYASDHGEKKLISVDTEHSNIYLQNGPD RIGRLYKKALYLQYTDETFRTTIEKPVWLGFLGPIIKAETGDKVYVHLKNLASRPYTFHS HGITYYKEHE corresponding to amino acids 1-131 of CERU_HUMAN, which also corresponds to amino acids 1-131 of HSCP2_PEA_(—)1_P22 (SEQ ID NO: 853), a second amino acid sequence bridging amino acid sequence comprising of A, and a third amino acid sequence being at least 90% homologous to VNGYTFGSLPGLSMCAEDRVKWYLFGMGNEVDVHAAFFHGQALTNKNYRIDTINLFP ATLFDAYMVAQNPGEWMLSCQNLNHLKAGLQAFFQVQECNKSSSKDNIRGKHVRHY YIAAEEIIWNYAPSGIDIFTKENLTAPGSDSAVFFEQGTTRIGGSYKKLVYREYTDASFTN RKERGPEEEHLGILGPVIWAEVGDTIRVTFHNKGAYPLSIEPIGVRFNKNNEGTYYSPNY NPQSRSVPPSASHVAPTETFTYEWTVPKEVGPTNADPVCLAKMYYSAVDPTKDIFTGLI GPMKICKKGSLHANGRQKDVDKEFYLFPTVFDENESLLLEDNIRMFTTAPDQVDKEDE DFQESNKMHSMNGFMYGNQPGLTMCKGDSVVWYLFSAGNEADVHGIYFSGNTYLWR GERRDTANLFPQTSLTLHMWPDTEGTFNVECLTTDHYTGGMKQKYTVNQCRRQSEDS TFYLGERTYYIAAVEVEWDYSPQREWEKELHHLQEQNVSNAFLDKGEFYIGSKYKKVV YRQYTDSTFRVPVERKAEEEHLGILGPQLHADVGDKVKIIFKNMATRPYSIHAHGVQTE SSTVTPTLPGETLTYVWKIPERSGAGTEDSACIPWAYYSTVDQVKDLYSGLIGPLIVCRR PYLKVFNPRRKLEFALLFLVFDENESWYLDDNIKTYSDHPEKVNKDDEEFIESNKMHAI NGRMFGNLQGLTMHVGDEVNWYLMGMGNEIDLHTVHFHGHSFQYKHRGVYSSDVF DIFPGTYQTLEMFPRTPGIWLLHCHVTDHIHAGMETTYTVLQNEDTKSG corresponding to amino acids 262-1065 of CERU_HUMAN, which also corresponds to amino acids 133-936 of HSCP2_PEA_(—)1_P22 (SEQ ID NO: 853), wherein said first amino acid sequence, second amino acid sequence and third amino acid sequence are contiguous and in a sequential order.

According to preferred embodiments of the present invention, there is provided an isolated polypeptide encoding for an edge portion of HSCP2_PEA_(—)1_P22 (SEQ ID NO: 853), comprising a polypeptide having a length “n”, wherein n is at least about 10 amino acids in length, optionally at least about 20 amino acids in length, preferably at least about 30 amino acids in length, more preferably at least about 40 amino acids in length and most preferably at least about 50 amino acids in length, wherein at least two amino acids comprise EAV having a structure as follows (numbering according to HSCP2_PEA_(—)1_P22 (SEQ ID NO: 853)): a sequence starting from any of amino acid numbers 131-x to 131; and ending at any of amino acid numbers 133+((n−2)−x), in which x varies from 0 to n−2.

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for HSCP2_PEA_(—)1_P24 (SEQ ID NO: 854), comprising a first amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence MPLTMGKRNLFLLTP (SEQ ID NO: 1095) corresponding to amino acids 1-15 of HSCP2_PEA_(—)1_P24 (SEQ ID NO: 854), and a second amino acid sequence being at least 90% homologous to VNGYTFGSLPGLSMCAEDRVKWYLFGMGNEVDVHAAFFHGQALTNKNYRIDTINLFP ATLFDAYMVAQNPGEWMLSCQNLNHLKAGLQAFFQVQECNKSSSKDNIRGKHVRHY YIAAEEIIWNYAPSGIDIFTKENLTAPGSDSAVFFEQGTTRIGGSYKKLVYREYTDASFTN RKERGPEEEHLGILGPVIWAEVGDTIRVTFHNKGAYPLSIEPIGVRFNKNNEGTYYSPNY NPQSRSVPPSASHVAPTETFTYEWTVPKEVGPTNADPVCLAKMYYSAVDPTKDIFTGLI GPMKICKKGSLHANGRQKDVDKEFYLFPTVFDENESLLLEDNIRMFTTAPDQVDKEDE DFQESNKMHSMNGFMYGNQPGLTMCKGDSVVWYLFSAGNEADVHGIYFSGNTYLWR GERRDTANLFPQTSLTLHMWPDTEGTFNVECLTTDHYTGGMKQKYTVNQCRRQSEDS TFYLGERTYYIAAVEVEWDYSPQREWEKELHHLQEQNVSNAFLDKGEFYIGSKYKKVV YRQYTDSTFRVPVERKAEEEHLGILGPQLHADVGDKVKIIFKNMATRPYSIHAHGVQTE SSTVTPTLPGETLTYVWKIPERSGAGTEDSACIPWAYYSTVDQVKDLYSGLIGPLIVCRR PYLKVFNPRRKLEFALLFLVFDENESWYLDDNIKTYSDHPEKVNKDDEEFIESNKMHAI NGRMFGNLQGLTMHVGDEVNWYLMGMGNEIDLHTVHFHGHSFQYKHRGVYSSDVF DIFPGTYQTLEMFPRTPGIWLLHCHVTDHIHAGMETTYTVLQNEDTKSG corresponding to amino acids 262-1065 of CERU_HUMAN, which also corresponds to amino acids 16-819 of HSCP2_PEA_(—)1_P24 (SEQ ID NO: 854), wherein said first amino acid sequence and second amino acid sequence are contiguous and in a sequential order.

According to preferred embodiments of the present invention, there is provided an isolated polypeptide encoding for a head of HSCP2_PEA_(—)1_P24 (SEQ ID NO: 854), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence MPLTMGKRNLFLLTP (SEQ ID NO: 1095) of HSCP2_PEA_(—)1_P24 (SEQ ID NO: 854).

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for HSCP2_PEA_(—)1_P25 (SEQ ID NO: 855), comprising a first amino acid sequence being at least 90% homologous to MKILILGIFLFLCSTPAWAKEKHYYIGIIETTWDYASDHGEKKLISVDTEHSNIYLQNGPD RIGRLYKKALYLQYTDETFRTTIEKPVWLGFLGPIIKAETGDKVYVHLKNLASRPYTFHS HGITYYKEHEGAIYPDNTTDFQRADDKVYPGEQYTYMLLATEEQSPGEGDGNCVTRIY HSHIDAPKDIASGLIGPLIICKKDSLDKEKEKHIDREFVVMFSVVDENFSWYLEDNIKTY CSEPEKVDKDNEDFQESNRMYSVNGYTFGSLPGLSMCAEDRVKWYLFGMGNEVDVH AAFFHGQALTNKNYRIDTINLFPATLFDAYMVAQNPGEWMLSCQNLNHLKAGLQAFF QVQECNKSSSKDNIRGKHVRHYYIAAEEIIWNYAPSGIDIFTKENLTAPGSDSAVFFEQG TTRIGGSYKKLVYREYTDASFTNRKERGPEEEHLGILGPVIWAEVGDTIRVTFHNKGAY PLSIEPIGVRFNKNNEGTYYSPNYNPQSRSVPPSASHVAPTETFTYEWTVPKEVGPTNAD PVCLAKMYYSAVDPTKDIFTGLIGPMKICKKGSLHANGRQKDVDKEFYLFPTVFDENES LLLEDNIRMFTTAPDQVDKEDEDFQESNKMH corresponding to amino acids 1-621 of CERU_HUMAN, which also corresponds to amino acids 1-621 of HSCP2_PEA_(—)1_P25 (SEQ ID NO: 855), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence CKYCIIHQSTKLF (SEQ ID NO: 1096) corresponding to amino acids 622-634 of HSCP2_PEA_(—)1_P25 (SEQ ID NO: 855), wherein said first amino acid sequence and second amino acid sequence are contiguous and in a sequential order.

According to preferred embodiments of the present invention, there is provided an isolated polypeptide encoding for a tail of HSCP2_PEA_(—)1_P25 (SEQ ID NO: 855), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence CKYCIIHQSTKLF (SEQ ID NO: 1096) in HSCP2_PEA_(—)1_P25 (SEQ ID NO: 855).

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for HSCP2_PEA_(—)1_P33 (SEQ ID NO: 856), comprising a first amino acid sequence being at least 90% homologous to MKILILGIFLFLCSTPAWAKEKHYYIGIIETTWDYASDHGEKKLISVDTEHSNIYLQNGPD RIGRLYKKALYLQYTDETFRTTIEKPVWLGFLGPIIKAETGDKVYVHLKNLASRPYTFHS HGITYYKEHEGAIYPDNTTDFQRADDKVYPGEQYTYMLLATEEQSPGEGDGNCVTRIY HSHIDAPKDIASGLIGPLIICKK corresponding to amino acids 1-202 of CERU_HUMAN, which also corresponds to amino acids 1-202 of HSCP2_PEA_(—)1_P33 (SEQ ID NO: 856), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence GTSSPYCTCYMTKRQGQGSLSFKKKSSLLC (SEQ ID NO: 1097) corresponding to amino acids 203-232 of HSCP2_PEA_(—)1_P33 (SEQ ID NO: 856), wherein said first amino acid sequence and second amino acid sequence are contiguous and in a sequential order.

According to preferred embodiments of the present invention, there is provided an isolated polypeptide encoding for a tail of HSCP2_PEA_(—)1_P33 (SEQ ID NO: 856), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence GTSSPYCTCYMTKRQGQGSLSFKKKSSLLC (SEQ ID NO: 1097) in HSCP2_PEA_(—)1_P33 (SEQ ID NO: 856).

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for HUMTEN_PEA_(—)1_P5 (SEQ ID NO: 934), comprising a first amino acid sequence being at least 90% homologous to MGAMTQLLAGVFLAFLALATEGGVLKKVIRHKRQSGVNATLPEENQPVVFNHVYNIK LPVGSQCSVDLESASGEKDLAPPSEPSESFQEHTVDGENQIVFTHRINIPRRACGCAAAP DVKELLSRLEELENLVSSLREQCTAGAGCCLQPATGRLDTRPFCSGRGNFSTEGCGCVC EPGWKGPNCSEPECPGNCHLRGRCIDGQCICDDGFTGEDCSQLACPSDCNDQGKCVNG VCICFEGYAGADCSREICPVPCSEEHGTCVDGLCVCHDGFAGDDCNKPLCLNNCYNRG RCVENECVCDEGFTGEDCSELICPNDCFDRGRCINGTCYCEEGFTGEDCGKPTCPHACH TQGRCEEGQCVCDEGFAGVDCSEKRCPADCHNRGRCVDGRCECDDGFTGADCGELKC PNGCSGHGRCVNGQCVCDEGYTGEDCSQLRCPNDCHSRGRCVEGKCVCEQGFKGYDC SDMSCPNDCHQHGRCVNGMCVCDDGYTGEDCRDRQCPRDCSNRGLCVDGQCVCEDG FTGPDCAELSCPNDCHGRGRCVNGQCVCHEGFMGKDCKEQRCPSDCHGQGRCVDGQ CICHEGFTGLDCGQHSCPSDCNNLGQCVSGRCICNEGYSGEDCSEVSPPKDLVVTEVTE ETVNLAWDNEMRVTEYLVVYTPTHEGGLEMQFRVPGDQTSTIIQELEPGVEYFIRVFAI LENKKSIPVSARVATYLPAPEGLKFKSIKETSVEVEWDPLDIAFETWEIIFRNMNKEDEG EITKSLRRPETSYRQTGLAPGQEYEISLHIVKNNTRGPGLKRVTTTRLDAPSQIEVKDVT DTTALITWFKPLAEIDGIELTYGIKDVPGDRTTIDLTEDENQYSIGNLKPDTEYEVSLISRR GDMSSNPAKETFTTGLDAPRNLRRVSQTDNSITLEWRNGKAAIDSYRIKYAPISGGDHA EVDVPKSQQATTKTTLTGLRPGTEYGIGVSAVKEDKESNPATINAATELDTPKDLQVSE TAETSLTLLWKTPLAKFDRYRLNYSLPTGQWVGVQLPRNTTSYVLRGLEPGQEYNVLL TAEKGRHKSKPARVKASTEQAPELENLTVTEVGWDGLRLNWTAADQAYEHFIIQVQE ANKVEAARNLTVPGSLRAVDIPGLKAATPYTVSIYGVIQGYRTPVLSAEASTGETPNLG EVVVAEVGWDALKLNWTAPEGAYEYFFIQVQEADTVEAAQNLTVPGGLRSTDLPGLK AATHYTITIRGVTQDFSTTPLSVEVLTEEVPDMGNLTVTEVSWDALRLNWTTPDGTYD QFTIQVQEADQVEEAHNLTVPGSLRSMEIPGLRAGTPYTVTLHGEVRGHSTRPLAVEVV TEDLPQLGDLAVSEVGWDGLRLNWTAADNAYEHFVIQVQEVNKVEAAQNLTLPGSLR AVDIPGLEAATPYRVSIYGVIRGYRTPVLSAEASTAKEPEIGNLNVSDITPESFNLSWMA TDGIFETFTIEIIDSNRLLETVEYNISGAERTAHISGLPPSTDFIVYLSGLAPSIRTKTISATA T corresponding to amino acids 1-1525 of TENA_HUMAN_V1 (SEQ ID NO: 1011), which also corresponds to amino acids 1-1525 of HUMTEN_PEA_(—)1_P5 (SEQ ID NO: 934), a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence TEPKPQLGTLIFSNITPKSFNMSWTTQAGLFAKIVINVSDAHSLHESQQFTVSGDAKQAH ITGLVENTGYDVSVAGTTLAGDPTRPLTAFVI (SEQ ID NO: 1144) corresponding to amino acids 1526-1617 of HUMTEN_PEA_(—)1_P5 (SEQ ID NO: 934), and a third amino acid sequence being at least 90% homologous to TEALPLLENLTISDINPYGFTVSWMASENAFDSFLVTVVDSGKLLDPQEFTLSGTQRKLE LRGLITGIGYEVMVSGFTQGHQTKPLRAEIVTEAEPEVDNLLVSDATPDGFRLSWTADE GVFDNFVLKIRDTKKQSEPLEITLLAPERTRDLTGLREATEYEIELYGISKGRRSQTVSAI ATTAMGSPKEVIFSDITENSATVSWRAPTAQVESFRITYVPITGGTPSMVTVDGTKTQTR LVKLIPGVEYLVSIIAMKGFEESEPVSGSFTTALDGPSGLVTANITDSEALARWQPAIATV DSYVISYTGEKVPEITRTVSGNTVEYALTDLEPATEYTLRIFAEKGPQKSSTITAKFTTDL DSPRDLTATEVQSETALLTWRPPRASVTGYLLVYESVDGTVKEVIVGPDTTSYSLADLS PSTHYTAKIQALNGPLRSNMIQTIFTTIGLLYPFPKDCSQAMLNGDTTSGLYTIYLNGDK AQALEVFCDMTSDGGGWIVFLRRKNGRENFYQNWKAYAAGFGDRREEFWLGLDNLN KITAQGQYELRVDLRDHGETAFAVYDKFSVGDAKTRYKLKVEGYSGTAGDSMAYHN GRSFSTFDKDTDSAITNCALSYKGAFWYRNCHRVNLMGRYGDNNHSQGVNWFHWKG HEHSIQFAEMKLRPSNFRNLEGRRKRA corresponding to amino acids 1526-2201 of TENA_HUMAN_V1 (SEQ ID NO: 1011), which also corresponds to amino acids 1618-2293 of HUMTEN_PEA_(—)1_P5 (SEQ ID NO: 934), wherein said first amino acid sequence, second amino acid sequence and third amino acid sequence are contiguous and in a sequential order.

According to preferred embodiments of the present invention, there is provided an isolated polypeptide encoding for an edge portion of HUMTEN_PEA_(—)1_P5 (SEQ ID NO: 934), comprising an amino acid sequence being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence encoding for TEPKPQLGTLIFSNITPKSFNMSWTTQAGLFAKIVINVSDAHSLHESQQFTVSGDAKQAH ITGLVENTGYDVSVAGTTLAGDPTRPLTAFVI (SEQ ID NO: 1144), corresponding to HUMTEN_PEA_(—)1_P5 (SEQ ID NO: 934).

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for HUMTEN_PEA_(—)1_P6 (SEQ ID NO: 935), comprising a first amino acid sequence being at least 90% homologous to MGAMTQLLAGVFLAFLALATEGGVLKKVIRHKRQSGVNATLPEENQPVVFNHVYNIK LPVGSQCSVDLESASGEKDLAPPSEPSESFQEHTVDGENQIVFTHRINIPRRACGCAAAP DVKELLSRLEELENLVSSLREQCTAGAGCCLQPATGRLDTRPFCSGRGNFSTEGCGCVC EPGWKGPNCSEPECPGNCHLRGRCIDGQCICDDGFTGEDCSQLACPSDCNDQGKCVNG VCICFEGYAGADCSREICPVPCSEEHGTCVDGLCVCHDGFAGDDCNKPLCLNNCYNRG RCVENECVCDEGFTGEDCSELICPNDCFDRGRCINGTCYCEEGFTGEDCGKPTCPHACH TQGRCEEGQCVCDEGFAGVDCSEKRCPADCHNRGRCVDGRCECDDGFTGADCGELKC PNGCSGHGRCVNGQCVCDEGYTGEDCSQLRCPNDCHSRGRCVEGKCVCEQGFKGYDC SDMSCPNDCHQHGRCVNGMCVCDDGYTGEDCRDRQCPRDCSNRGLCVDGQCVCEDG FTGPDCAELSCPNDCHGRGRCVNGQCVCHEGFMGKDCKEQRCPSDCHGQGRCVDGQ CICHEGFTGLDCGQHSCPSDCNNLGQCVSGRCICNEGYSGEDCSEVSPPKDLVVTEVTE ETVNLAWDNEMRVTEYLVVYTPTHEGGLEMQFRVPGDQTSTIIQELEPGVEYFIRVFAI LENKKSIPVSARVATYLPAPEGLKFKSIKETSVEVEWDPLDIAFETWEIIFRNMNKEDEG EITKSLRRPETSYRQTGLAPGQEYEISLHIVKNNTRGPGLKRVTTTRLDAPSQIEVKDVT DTTALITWFKPLAEIDGIELTYGIKDVPGDRTTIDLTEDENQYSIGNLKPDTEYEVSLISRR GDMSSNPAKETFTTGLDAPRNLRRVSQTDNSITLEWRNGKAAIDSYRIKYAPISGGDHA EVDVPKSQQATTKTTLTGLRPGTEYGIGVSAVKEDKESNPATINAATELDTPKDLQVSE TAETSLTLLWKTPLAKFDRYRLNYSLPTGQWVGVQLPRNTTSYVLRGLEPGQEYNVLL TAEKGRHKSKPARVKASTEQAPELENLTVTEVGWDGLRLNWTAADQAYEHFIIQVQE ANKVEAARNLTVPGSLRAVDIPGLKAATPYTVSIYGVIQGYRTPVLSAEASTGETPNLG EVVVAEVGWDALKLNWTAPEGAYEYFFIQVQEADTVEAAQNLTVPGGLRSTDLPGLK AATHYTITIRGVTQDFSTTPLSVEVLTEEVPDMGNLTVTEVSWDALRLNWTTPDGTYD QFTIQVQEADQVEEAHNLTVPGSLRSMEIPGLRAGTPYTVTLHGEVRGHSTRPLAVEVV TEDLPQLGDLAVSEVGWDGLRLNWTAADNAYEHFVIQVQEVNKVEAAQNLTLPGSLR AVDIPGLEAATPYRVSIYGVIRGYRTPVLSAEASTAKEPEIGNLNVSDITPESFNLSWMA TDGIFETFTIEIIDSNRLLETVEYNISGAERTAHISGLPPSTDFIVYLSGLAPSIRTKTISATA TTE corresponding to amino acids 1-1527 of TENA_HUMAN_V1 (SEQ ID NO: 1011), which also corresponds to amino acids 1-1527 of HUMTEN_PEA_(—)1_P6 (SEQ ID NO: 935), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence PKPQLGTLIFSNITPKSFNMSWTTQAGLFAKIVINVSDAHSLHESQQFTVSGDAKQAHIT GLVENTGYDVSVAGTTLAGDPTRPLTAFVITGTQSEVLTCLTQREKEISHLKGKFNKNTI FTANVYSLIFN (SEQ ID NO: 1098) corresponding to amino acids 1528-1658 of HUMTEN_PEA_(—)1_P6 (SEQ ID NO: 935), wherein said first amino acid sequence and second amino acid sequence are contiguous and in a sequential order.

According to preferred embodiments of the present invention, there is provided an isolated polypeptide encoding for a tail of HUMTEN_PEA_(—)1_P6 (SEQ ID NO: 935), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence PKPQLGTLIFSNITPKSFNMSWTTQAGLFAKIVINVSDAHSLHESQQFTVSGDAKQAHIT GLVENTGYDVSVAGTTLAGDPTRPLTAFVITGTQSEVLTCLTQREKEISHLKGKFNKNTI FTANVYSLIFN (SEQ ID NO: 1098) in HUMTEN_PEA_(—)1_P6 (SEQ ID NO: 935).

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for HUMTEN_PEA_(—)1_P7 (SEQ ID NO: 936), comprising a first amino acid sequence being at least 90% homologous to MGAMTQLLAGVFLAFLALATEGGVLKKVIRHKRQSGVNATLPEENQPVVFNHVYNIK LPVGSQCSVDLESASGEKDLAPPSEPSESFQEHTVDGENQIVFTHRINIPRRACGCAAAP DVKELLSRLEELENLVSSLREQCTAGAGCCLQPATGRLDTRPFCSGRGNFSTEGCGCVC EPGWKGPNCSEPECPGNCHLRGRCIDGQCICDDGFTGEDCSQLACPSDCNDQGKCVNG VCICFEGYAGADCSREICPVPCSEEHGTCVDGLCVCHDGFAGDDCNKPLCLNNCYNRG RCVENECVCDEGFTGEDCSELICPNDCFDRGRCINGTCYCEEGFTGEDCGKPTCPHACH TQGRCEEGQCVCDEGFAGVDCSEKRCPADCHNRGRCVDGRCECDDGFTGADCGELKC PNGCSGHGRCVNGQCVCDEGYTGEDCSQLRCPNDCHSRGRCVEGKCVCEQGFKGYDC SDMSCPNDCHQHGRCVNGMCVCDDGYTGEDCRDRQCPRDCSNRGLCVDGQCVCEDG FTGPDCAELSCPNDCHGRGRCVNGQCVCHEGFMGKDCKEQRCPSDCHGQGRCVDGQ CICHEGFTGLDCGQHSCPSDCNNLGQCVSGRCICNEGYSGEDCSEVSPPKDLVVTEVTE ETVNLAWDNEMRVTEYLVVYTPTHEGGLEMQFRVPGDQTSTIIQELEPGVEYFIRVFAI LENKKSIPVSARVATYLPAPEGLKFKSIKETSVEVEWDPLDIAFETWEIIFRNMNKEDEG EITKSLRRPETSYRQTGLAPGQEYEISLHIVKNNTRGPGLKRVTTTRLDAPSQIEVKDVT DTTALITWFKPLAEIDGIELTYGIKDVPGDRTTIDLTEDENQYSIGNLKPDTEYEVSLISRR GDMSSNPAKETFTTGLDAPRNLRRVSQTDNSITLEWRNGKAAIDSYRIKYAPISGGDHA EVDVPKSQQATTKTTLTGLRPGTEYGIGVSAVKEDKESNPATINAATELDTPKDLQVSE TAETSLTLLWKTPLAKFDRYRLNYSLPTGQWVGVQLPRNTTSYVLRGLEPGQEYNVLL TAEKGRHKSKPARVKASTEQAPELENLTVTEVGWDGLRLNWTAADQAYEHFIIQVQE ANKVEAARNLTVPGSLRAVDIPGLKAATPYTVSIYGVIQGYRTPVLSAEASTGETPNLG EVVVAEVGWDALKLNWTAPEGAYEYFFIQVQEADTVEAAQNLTVPGGLRSTDLPGLK AATHYTITIRGVTQDFSTTPLSVEVLTEEVPDMGNLTVTEVSWDALRLNWTTPDGTYD QFTIQVQEADQVEEAHNLTVPGSLRSMEIPGLRAGTPYTVTLHGEVRGHSTRPLAVEVV TEDLPQLGDLAVSEVGWDGLRLNWTAADNAYEHFVIQVQEVNKVEAAQNLTLPGSLR AVDIPGLEAATPYRVSIYGVIRGYRTPVLSAEASTAKEPEIGNLNVSDITPESFNLSWMA TDGIFETFTIEIIDSNRLLETVEYNISGAERTAHISGLPPSTDFIVYLSGLAPSIRTKTISATA TTEALPLLENLTISDINPYGFTVSWMASENAFDSFLVTVVDSGKLLDPQEFTLSGTQRKL ELRGLITGIGYEVMVSGFTQGHQTKPLRAEIVT corresponding to amino acids 1-1617 of TENA_HUMAN_V1 (SEQ ID NO: 1011), which also corresponds to amino acids 1-1617 of HUMTEN_PEA_(—)1_P7 (SEQ ID NO: 936), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence GISNQVSHLFLFLVPFCVICLPDRHDFNIFVHIPYLIHKCSLLFHLLPTLPLVICT (SEQ ID NO: 1099) corresponding to amino acids 1618-1673 of HUMTEN_PEA_(—)1_P7 (SEQ ID NO: 936), wherein said first amino acid sequence and second amino acid sequence are contiguous and in a sequential order.

According to preferred embodiments of the present invention, there is provided an isolated polypeptide encoding for a tail of HUMTEN_PEA_(—)1_P7 (SEQ ID NO: 936), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence GISNQVSHLFLFLVPFCVICLPDRHDFNIFVHIPYLIHKCSLLFHLLPTLPLVICT in (SEQ ID NO: 1099) HUMTEN_PEA_(—)1_P7 (SEQ ID NO: 936).

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for HUMTEN_PEA_(—)1_P8 (SEQ ID NO: 937), comprising a first amino acid sequence being at least 90% homologous to MGAMTQLLAGVFLAFLALATEGGVLKKVIRHKRQSGVNATLPEENQPVVFNHVYNIK LPVGSQCSVDLESASGEKDLAPPSEPSESFQEHTVDGENQIVFTHRINIPRRACGCAAAP DVKELLSRLEELENLVSSLREQCTAGAGCCLQPATGRLDTRPFCSGRGNFSTEGCGCVC EPGWKGPNCSEPECPGNCHLRGRCIDGQCICDDGFTGEDCSQLACPSDCNDQGKCVNG VCICFEGYAGADCSREICPVPCSEEHGTCVDGLCVCHDGFAGDDCNKPLCLNNCYNRG RCVENECVCDEGFTGEDCSELICPNDCFDRGRCINGTCYCEEGFTGEDCGKPTCPHACH TQGRCEEGQCVCDEGFAGVDCSEKRCPADCHNRGRCVDGRCECDDGFTGADCGELKC PNGCSGHGRCVNGQCVCDEGYTGEDCSQLRCPNDCHSRGRCVEGKCVCEQGFKGYDC SDMSCPNDCHQHGRCVNGMCVCDDGYTGEDCRDRQCPRDCSNRGLCVDGQCVCEDG FTGPDCAELSCPNDCHGRGRCVNGQCVCHEGFMGKDCKEQRCPSDCHGQGRCVDGQ CICHEGFTGLDCGQHSCPSDCNNLGQCVSGRCICNEGYSGEDCSEVSPPKDLVVTEVTE ETVNLAWDNEMRVTEYLVVYTPTHEGGLEMQFRVPGDQTSTIIQELEPGVEYFIRVFAI LENKKSIPVSARVATYLPAPEGLKFKSIKETSVEVEWDPLDIAFETWEIIFRNMNKEDEG EITKSLRRPETSYRQTGLAPGQEYEISLHIVKNNTRGPGLKRVTTTRLDAPSQIEVKDVT DTTALITWFKPLAEIDGIELTYGIKDVPGDRTTIDLTEDENQYSIGNLKPDTEYEVSLISRR GDMSSNPAKETFTTGLDAPRNLRRVSQTDNSITLEWRNGKAAIDSYRIKYAPISGGDHA EVDVPKSQQATTKTTLTGLRPGTEYGIGVSAVKEDKESNPATINAATELDTPKDLQVSE TAETSLTLLWKTPLAKFDRYRLNYSLPTGQWVGVQLPRNTTSYVLRGLEPGQEYNVLL TAEKGRHKSKPARVKASTEQAPELENLTVTEVGWDGLRLNWTAADQAYEHFIIQVQE ANKVEAARNLTVPGSLRAVDIPGLKAATPYTVSIYGVIQGYRTPVLSAEASTGETPNLG EVVVAEVGWDALKLNWTAPEGAYEYFFIQVQEADTVEAAQNLTVPGGLRSTDLPGLK AATHYTITIRGVTQDFSTTPLSVEVLTEEVPDMGNLTVTEVSWDALRLNWTTPDGTYD QFTIQVQEADQVEEAHNLTVPGSLRSMEIPGLRAGTPYTVTLHGEVRGHSTRPLAVEVV TEDLPQLGDLAVSEVGWDGLRLNWTAADNAYEHFVIQVQEVNKVEAAQNLTLPGSLR AVDIPGLEAATPYRVSIYGVIRGYRTPVLSAEASTAKEPEIGNLNVSDITPESFNLSWMA TDGIFETFTIEIIDSNRLLETVEYNISGAERTAHISGLPPSTDFIVYLSGLAPSIRTKTISATA T corresponding to amino acids 1-1525 of TENA_HUMAN_V1 (SEQ ID NO: 1011), which also corresponds to amino acids 1-1525 of HUMTEN_PEA_(—)1_P8 (SEQ ID NO: 937), and a second amino acid sequence being at least 90% homologous to TEAEPEVDNLLVSDATPDGFRLSWTADEGVFDNFVLKIRDTKKQSEPLEITLLAPERTRD LTGLREATEYEIELYGISKGRRSQTVSAIATTAMGSPKEVIFSDITENSATVSWRAPTAQV ESFRITYVPITGGTPSMVTVDGTKTQTRLVKLIPGVEYLVSIIAMKGFEESEPVSGSFTTA LDGPSGLVTANITDSEALARWQPAIATVDSYVISYTGEKVPEITRTVSGNTVEYALTDLE PATEYTLRIFAEKGPQKSSTITAKFTTDLDSPRDLTATEVQSETALLTWRPPRASVTGYL LVYESVDGTVKEVIVGPDTTSYSLADLSPSTHYTAKIQALNGPLRSNMIQTIFTTIGLLYP FPKDCSQAMLNGDTTSGLYTIYLNGDKAQALEVFCDMTSDGGGWIVFLRRKNGRENF YQNWKAYAAGFGDRREEFWLGLDNLNKITAQGQYELRVDLRDHGETAFAVYDKFSV GDAKTRYKLKVEGYSGTAGDSMAYHNGRSFSTFDKDTDSAITNCALSYKGAFWYRNC HRVNLMGRYGDNNHSQGVNWFHWKGHEHSIQFAEMKLRPSNFRNLEGRRKRA corresponding to amino acids 1617-2201 of TENA_HUMAN_V1 (SEQ ID NO: 1011), which also corresponds to amino acids 1526-2110 of HUMTEN_PEA_(—)1_P8 (SEQ ID NO: 937), wherein said first amino acid sequence and second amino acid sequence are contiguous and in a sequential order.

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for an edge portion of HUMTEN_PEA_(—)1_P8 (SEQ ID NO: 937), comprising a polypeptide having a length “n”, wherein n is at least about 10 amino acids in length, optionally at least about 20 amino acids in length, preferably at least about 30 amino acids in length, more preferably at least about 40 amino acids in length and most preferably at least about 50 amino acids in length, wherein at least two amino acids comprise TT, having a structure as follows: a sequence starting from any of amino acid numbers 1525-x to 1525; and ending at any of amino acid numbers 1526+((n−2)−x), in which x varies from 0 to n−2.

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for HUMTEN_PEA_(—)1_P10 (SEQ ID NO: 938), comprising a first amino acid sequence being at least 90% homologous to MGAMTQLLAGVFLAFLALATEGGVLKKVIRHKRQSGVNATLPEENQPVVFNHVYNIK LPVGSQCSVDLESASGEKDLAPPSEPSESFQEHTVDGENQIVFTHRINIPRRACGCAAAP DVKELLSRLEELENLVSSLREQCTAGAGCCLQPATGRLDTRPFCSGRGNFSTEGCGCVC EPGWKGPNCSEPECPGNCHLRGRCIDGQCICDDGFTGEDCSQLACPSDCNDQGKCVNG VCICFEGYAGADCSREICPVPCSEEHGTCVDGLCVCHDGFAGDDCNKPLCLNNCYNRG RCVENECVCDEGFTGEDCSELICPNDCFDRGRCINGTCYCEEGFTGEDCGKPTCPHACH TQGRCEEGQCVCDEGFAGVDCSEKRCPADCHNRGRCVDGRCECDDGFTGADCGELKC PNGCSGHGRCVNGQCVCDEGYTGEDCSQLRCPNDCHSRGRCVEGKCVCEQGFKGYDC SDMSCPNDCHQHGRCVNGMCVCDDGYTGEDCRDRQCPRDCSNRGLCVDGQCVCEDG FTGPDCAELSCPNDCHGRGRCVNGQCVCHEGFMGKDCKEQRCPSDCHGQGRCVDGQ CICHEGFTGLDCGQHSCPSDCNNLGQCVSGRCICNEGYSGEDCSEVSPPKDLVVTEVTE ETVNLAWDNEMRVTEYLVVYTPTHEGGLEMQFRVPGDQTSTIIQELEPGVEYFIRVFAI LENKKSIPVSARVATYLPAPEGLKFKSIKETSVEVEWDPLDIAFETWEIIFRNMNKEDEG EITKSLRRPETSYRQTGLAPGQEYEISLHIVKNNTRGPGLKRVTTTRLDAPSQIEVKDVT DTTALITWFKPLAEIDGIELTYGIKDVPGDRTTIDLTEDENQYSIGNLKPDTEYEVSLISRR GDMSSNPAKETFTTGLDAPRNLRRVSQTDNSITLEWRNGKAAIDSYRIKYAPISGGDHA EVDVPKSQQATTKTTLTGLRPGTEYGIGVSAVKEDKESNPATINAATELDTPKDLQVSE TAETSLTLLWKTPLAKFDRYRLNYSLPTGQWVGVQLPRNTTSYVLRGLEPGQEYNVLL TAEKGRHKSKPARVKASTEQAPELENLTVTEVGWDGLRLNWTAADQAYEHFIIQVQE ANKVEAARNLTVPGSLRAVDIPGLKAATPYTVSIYGVIQGYRTPVLSAEASTGETPNLG EVVVAEVGWDALKLNWTAPEGAYEYFFIQVQEADTVEAAQNLTVPGGLRSTDLPGLK AATHYTITIRGVTQDFSTTPLSVEVL corresponding to amino acids 1-1252 of TENA_HUMAN_V1 (SEQ ID NO: 1011), which also corresponds to amino acids 1-1252 of HUMTEN_PEA_(—)1_P10 (SEQ ID NO: 938), and a second amino acid sequence being at least 90% homologous to TEDLPQLGDLAVSEVGWDGLRLNWTAADNAYEHFVIQVQEVNKVEAAQNLTLPGSLR AVDIPGLEAATPYRVSIYGVIRGYRTPVLSAEASTAKEPEIGNLNVSDITPESFNLSWMA TDGIFETFTIEIIDSNRLLETVEYNISGAERTAHISGLPPSTDFIVYLSGLAPSIRTKTISATA TTEALPLLENLTISDINPYGFTVSWMASENAFDSFLVTVVDSGKLLDPQEFTLSGTQRKL ELRGLITGIGYEVMVSGFTQGHQTKPLRAEIVTEAEPEVDNLLVSDATPDGFRLSWTAD EGVFDNFVLKIRDTKKQSEPLEITLLAPERTRDLTGLREATEYEIELYGISKGRRSQTVSA IATTAMGSPKEVIFSDITENSATVSWRAPTAQVESFRITYVPITGGTPSMVTVDGTKTQT RLVKLIPGVEYLVSIIAMKGFEESEPVSGSFTTALDGPSGLVTANITDSEALARWQPAIAT VDSYVISYTGEKVPEITRTVSGNTVEYALTDLEPATEYTLRIFAEKGPQKSSTITAKFTTD LDSPRDLTATEVQSETALLTWRPPRASVTGYLLVYESVDGTVKEVIVGPDTTSYSLADL SPSTHYTAKIQALNGPLRSNMIQTIFTTIGLLYPFPKDCSQAMLNGDTTSGLYTIYLNGD KAQALEVFCDMTSDGGGWIVFLRRKNGRENFYQNWKAYAAGFGDRREEFWLGLDNL NKITAQGQYELRVDLRDHGETAFAVYDKFSVGDAKTRYKLKVEGYSGTAGDSMAYH NGRSFSTFDKDTDSAITNCALSYKGAFWYRNCHRVNLMGRYGDNNHSQGVNWFHWK GHEHSIQFAEMKLRPSNFRNLEGRRKRA corresponding to amino acids 1344-2201 of TENA_HUMAN_V1 (SEQ ID NO: 1011), which also corresponds to amino acids 1253-2110 of HUMTEN_PEA_(—)1_P10 (SEQ ID NO: 938), wherein said first amino acid sequence and second amino acid sequence are contiguous and in a sequential order.

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for an edge portion of HUMTEN_PEA_(—)1_P10 (SEQ ID NO: 938), comprising a polypeptide having a length “n”, wherein n is at least about 10 amino acids in length, optionally at least about 20 amino acids in length, preferably at least about 30 amino acids in length, more preferably at least about 40 amino acids in length and most preferably at least about 50 amino acids in length, wherein at least two amino acids comprise LT, having a structure as follows: a sequence starting from any of amino acid numbers 1252-x to 1252; and ending at any of amino acid numbers 1253+((n−2)−x), in which x varies from 0 to n−2.

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for HUMTEN_PEA_(—)1_P13 (SEQ ID NO: 940), comprising a first amino acid sequence being at least 90% homologous to MGAMTQLLAGVFLAFLALATEGGVLKKVIRHKRQSGVNATLPEENQPVVFNHVYNIK LPVGSQCSVDLESASGEKDLAPPSEPSESFQEHTVDGENQIVFTHRINIPRRACGCAAAP DVKELLSRLEELENLVSSLREQCTAGAGCCLQPATGRLDTRPFCSGRGNFSTEGCGCVC EPGWKGPNCSEPECPGNCHLRGRCIDGQCICDDGFTGEDCSQLACPSDCNDQGKCVNG VCICFEGYAGADCSREICPVPCSEEHGTCVDGLCVCHDGFAGDDCNKPLCLNNCYNRG RCVENECVCDEGFTGEDCSELICPNDCFDRGRCINGTCYCEEGFTGEDCGKPTCPHACH TQGRCEEGQCVCDEGFAGVDCSEKRCPADCHNRGRCVDGRCECDDGFTGADCGELKC PNGCSGHGRCVNGQCVCDEGYTGEDCSQLRCPNDCHSRGRCVEGKCVCEQGFKGYDC SDMSCPNDCHQHGRCVNGMCVCDDGYTGEDCRDRQCPRDCSNRGLCVDGQCVCEDG FTGPDCAELSCPNDCHGRGRCVNGQCVCHEGFMGKDCKEQRCPSDCHGQGRCVDGQ CICHEGFTGLDCGQHSCPSDCNNLGQCVSGRCICNEGYSGEDCSEVSPPKDLVVTEVTE ETVNLAWDNEMRVTEYLVVYTPTHEGGLEMQFRVPGDQTSTIIQELEPGVEYFIRVFAI LENKKSIPVSARVATYLPAPEGLKFKSIKETSVEVEWDPLDIAFETWEIIFRNMNKEDEG EITKSLRRPETSYRQTGLAPGQEYEISLHIVKNNTRGPGLKRVTTTRLDAPSQIEVKDVT DTTALITWFKPLAEIDGIELTYGIKDVPGDRTTIDLTEDENQYSIGNLKPDTEYEVSLISRR GDMSSNPAKETFTTGLDAPRNLRRVSQTDNSITLEWRNGKAAIDSYRIKYAPISGGDHA EVDVPKSQQATTKTTLTGLRPGTEYGIGVSAVKEDKESNPATINAATELDTPKDLQVSE TAETSLTLLWKTPLAKFDRYRLNYSLPTGQWVGVQLPRNTTSYVLRGLEPGQEYNVLL TAEKGRHKSKPARVKASTEQAPELENLTVTEVGWDGLRLNWTAADQAYEHFIIQVQE ANKVEAARNLTVPGSLRAVDIPGLKAATPYTVSIYGVIQGYRTPVLSAEASTGETPNLG EVVVAEVGWDALKLNWTAPEGAYEYFFIQVQEADTVEAAQNLTVPGGLRSTDLPGLK AATHYTITIRGVTQDFSTTPLSVEVLTEEVPDMGNLTVTEVSWDALRLNWTTPDGTYD QFTIQVQEADQVEEAHNLTVPGSLRSMEIPGLRAGTPYTVTLHGEVRGHSTRPLAVEVV corresponding to amino acids 1-1343 of TENA_HUMAN_V1 (SEQ ID NO: 1011), which also corresponds to amino acids 1-1343 of HUMTEN_PEA_(—)1_P13 (SEQ ID NO: 940), and a second amino acid sequence being at least 90% homologous to TAMGSPKEVIFSDITENSATVSWRAPTAQVESFRITYVPITGGTPSMVTVDGTKTQTRLV KLIPGVEYLVSIIAMKGFEESEPVSGSFTTALDGPSGLVTANITDSEALARWQPAIATVDS YVISYTGEKVPEITRTVSGNTVEYALTDLEPATEYTLRIFAEKGPQKSSTITAKFTTDLDS PRDLTATEVQSETALLTWRPPRASVTGYLLVYESVDGTVKEVIVGPDTTSYSLADLSPS THYTAKIQALNGPLRSNMIQTIFTTIGLLYPFPKDCSQAMLNGDTTSGLYTIYLNGDKAQ ALEVFCDMTSDGGGWIVFLRRKNGRENFYQNWKAYAAGFGDRREEFWLGLDNLNKIT AQGQYELRVDLRDHGETAFAVYDKFSVGDAKTRYKLKVEGYSGTAGDSMAYHNGRS FSTFDKDTDSAITNCALSYKGAFWYRNCHRVNLMGRYGDNNHSQGVNWFHWKGHEH SIQFAEMKLRPSNFRNLEGRRKRA corresponding to amino acids 1708-2201 of TENA_HUMAN_V1 (SEQ ID NO: 1011), which also corresponds to amino acids 1344-1837 of HUMTEN_PEA_(—)1_P13 (SEQ ID NO: 940), wherein said first amino acid sequence and second amino acid sequence are contiguous and in a sequential order.

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for an edge portion of HUMTEN_PEA_(—)1_P13 (SEQ ID NO: 940), comprising a polypeptide having a length “n”, wherein n is at least about 10 amino acids in length, optionally at least about 20 amino acids in length, preferably at least about 30 amino acids in length, more preferably at least about 40 amino acids in length and most preferably at least about 50 amino acids in length, wherein at least two amino acids comprise VT, having a structure as follows: a sequence starting from any of amino acid numbers 1343-x to 1343; and ending at any of amino acid numbers 1344+((n−2)−x), in which x varies from 0 to n−2.

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for HUMTEN_PEA_(—)1_P14 (SEQ ID NO: 941), comprising a first amino acid sequence being at least 90% homologous to MGAMTQLLAGVFLAFLALATEGGVLKKVIRHKRQSGVNATLPEENQPVVFNHVYNIK LPVGSQCSVDLESASGEKDLAPPSEPSESFQEHTVDGENQIVFTHRINIPRRACGCAAAP DVKELLSRLEELENLVSSLREQCTAGAGCCLQPATGRLDTRPFCSGRGNFSTEGCGCVC EPGWKGPNCSEPECPGNCHLRGRCIDGQCICDDGFTGEDCSQLACPSDCNDQGKCVNG VCICFEGYAGADCSREICPVPCSEEHGTCVDGLCVCHDGFAGDDCNKPLCLNNCYNRG RCVENECVCDEGFTGEDCSELICPNDCFDRGRCINGTCYCEEGFTGEDCGKPTCPHACH TQGRCEEGQCVCDEGFAGVDCSEKRCPADCHNRGRCVDGRCECDDGFTGADCGELKC PNGCSGHGRCVNGQCVCDEGYTGEDCSQLRCPNDCHSRGRCVEGKCVCEQGFKGYDC SDMSCPNDCHQHGRCVNGMCVCDDGYTGEDCRDRQCPRDCSNRGLCVDGQCVCEDG FTGPDCAELSCPNDCHGRGRCVNGQCVCHEGFMGKDCKEQRCPSDCHGQGRCVDGQ CICHEGFTGLDCGQHSCPSDCNNLGQCVSGRCICNEGYSGEDCSEVSPPKDLVVTEVTE ETVNLAWDNEMRVTEYLVVYTPTHEGGLEMQFRVPGDQTSTIIQELEPGVEYFIRVFAI LENKKSIPVSARVATYLPAPEGLKFKSIKETSVEVEWDPLDIAFETWEIIFRNMNKEDEG EITKSLRRPETSYRQTGLAPGQEYEISLHIVKNNTRGPGLKRVTTTRLDAPSQIEVKDVT DTTALITWFKPLAEIDGIELTYGIKDVPGDRTTIDLTEDENQYSIGNLKPDTEYEVSLISRR GDMSSNPAKETFTTGLDAPRNLRRVSQTDNSITLEWRNGKAAIDSYRIKYAPISGGDHA EVDVPKSQQATTKTTLTGLRPGTEYGIGVSAVKEDKESNPATINAATELDTPKDLQVSE TAETSLTLLWKTPLAKFDRYRLNYSLPTGQWVGVQLPRNTTSYVLRGLEPGQEYNVLL TAEKGRHKSKPARVKASTEQAPELENLTVTEVGWDGLRLNWTAADQAYEHFIIQVQE ANKVEAARNLTVPGSLRAVDIPGLKAATPYTVSIYGVIQGYRTPVLSAEASTGETPNLG EVVVAEVGWDALKLNWTAPEGAYEYFFIQVQEADTVEAAQNLTVPGGLRSTDLPGLK AATHYTITIRGVTQDFSTTPLSVEVLTEEVPDMGNLTVTEVSWDALRLNWTTPDGTYD QFTIQVQEADQVEEAHNLTVPGSLRSMEIPGLRAGTPYTVTLHGEVRGHSTRPLAVEVV TEDLPQLGDLAVSEVGWDGLRLNWTAADNAYEHFVIQVQEVNKVEAAQNLTLPGSLR AVDIPGLEAATPYRVSIYGVIRGYRTPVLSAEASTAKEPEIGNLNVSDITPESFNLSWMA TDGIFETFTIEIIDSNRLLETVEYNISGAERTAHISGLPPSTDFIVYLSGLAPSIRTKTISATA TTEALPLLENLTISDINPYGFTVSWMASENAFDSFLVTVVDSGKLLDPQEFTLSGTQRKL ELRGLITGIGYEVMVSGFTQGHQTKPLRAEIVTEAEPEVDNLLVSDATPDGFRLSWTAD EGVFDNFVLKIRDTKKQSEPLEITLLAPERTRDLTGLREATEYEIELYGISKGRRSQTVSA IATTAMGSPKEVIFSDITENSATVSWRAPTAQVESFRITYVPITGGTPSMVTVDGTKTQT RLVKLIPGVEYLVSIIAMKGFEESEPVSGSFTTALDGPSGLVTANITDSEALARWQPAIAT VDSYVISYTGEKVPEITRTVSGNTVEYALTDLEPATEYTLRIFAEKGPQKSSTITAKFTTD LDSPRDLTATEVQSETALLTWRPPRASVTGYLLVYESVDGTVKEVIVGPDTTSYSLADL SPSTHYTAKIQALNGPLRSNMIQTIFTTIGLLYPFPKDCSQAMLNGDTTSGLYTIYLNGD KAQALEVFCDMTSDGGGWW corresponding to amino acids 1-2025 of TENA_HUMAN_V1 (SEQ ID NO: 1011), which also corresponds to amino acids 1-2025 of HUMTEN_PEA_(—)1_P14 (SEQ ID NO: 941), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence STTRDCRALRPRGRGRGQSRGGEEGDLLLMHSDTPMCEALQDSACHTEALRNSLLNKR MGNTLATF (SEQ ID NO: 1100) corresponding to amino acids 2026-2091 of HUMTEN_PEA_(—)1_P14 (SEQ ID NO: 941), wherein said first amino acid sequence and second amino acid sequence are contiguous and in a sequential order.

According to preferred embodiments of the present invention, there is provided an isolated polypeptide encoding for a tail of HUMTEN_PEA_(—)1_P14 (SEQ ID NO: 941), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence STTRDCRALRPRGRGRGQSRGGEEGDLLLMHSDTPMCEALQDSACHTEALRNSLLNKR MGNTLATF (SEQ ID NO: 1100) in HUMTEN_PEA_(—)1_P14 (SEQ ID NO: 941).

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for HUMTEN_PEA_(—)1_P15 (SEQ ID NO: 942), comprising a first amino acid sequence being at least 90% homologous to MGAMTQLLAGVFLAFLALATEGGVLKKVIRHKRQSGVNATLPEENQPVVFNHVYNIK LPVGSQCSVDLESASGEKDLAPPSEPSESFQEHTVDGENQWFTHRINIPRRACGCAAAP DVKELLSRLEELENLVSSLREQCTAGAGCCLQPATGRLDTRPFCSGRGNFSTEGCGCVC EPGWKGPNCSEPECPGNCHLRGRCIDGQCICDDGFTGEDCSQLACPSDCNDQGKCVNG VCICFEGYAGADCSREICPVPCSEEHGTCVDGLCVCHDGFAGDDCNKPLCLNNCYNRG RCVENECVCDEGFTGEDCSELICPNDCFDRGRCINGTCYCEEGFTGEDCGKPTCPHACH TQGRCEEGQCVCDEGFAGVDCSEKRCPADCHNRGRCVDGRCECDDGFTGADCGELKC PNGCSGHGRCVNGQCVCDEGYTGEDCSQLRCPNDCHSRGRCVEGKCVCEQGFKGYDC SDMSCPNDCHQHGRCVNGMCVCDDGYTGEDCRDRQCPRDCSNRGLCVDGQCVCEDG FTGPDCAELSCPNDCHGRGRCVNGQCVCHEGFMGKDCKEQRCPSDCHGQGRCVDGQ CICHEGFTGLDCGQHSCPSDCNNLGQCVSGRCICNEGYSGEDCSEVSPPKDLVVTEVTE ETVNLAWDNEMRVTEYLVVYTPTHEGGLEMQFRVPGDQTSTIIQELEPGVEYFIRVFAI LENKKSIPVSARVATYLPAPEGLKFKSIKETSVEVEWDPLDIAFETWEIIFRNMNKEDEG EITKSLRRPETSYRQTGLAPGQEYEISLHIVKNNTRGPGLKRVTTTRLDAPSQIEVKDVT DTTALITWFKPLAEIDGIELTYGIKDVPGDRTTIDLTEDENQYSIGNLKPDTEYEVSLISRR GDMSSNPAKETFTTGLDAPRNLRRVSQTDNSITLEWRNGKAAIDSYRIKYAPISGGDHA EVDVPKSQQATTKTTLTGLRPGTEYGIGVSAVKEDKESNPATINAATELDTPKDLQVSE TAETSLTLLWKTPLAKFDRYRLNYSLPTGQWVGVQLPRNTTSYVLRGLEPGQEYNVLL TAEKGRHKSKPARVKAS corresponding to amino acids 1-1070 of TENA_HUMAN_V1 (SEQ ID NO: 1011), which also corresponds to amino acids 1-1070 of HUMTEN_PEA_(—)1_P15 (SEQ ID NO: 942), and a second amino acid sequence being at least 90% homologous to TEAEPEVDNLLVSDATPDGFRLSWTADEGVFDNFVLKIRDTKKQSEPLEITLLAPERTRD LTGLREATEYEIELYGISKGRRSQTVSAIATTAMGSPKEVIFSDITENSATVSWRAPTAQV ESFRITYVPITGGTPSMVTVDGTKTQTRLVKLIPGVEYLVSIIAMKGFEESEPVSGSFTTA LDGPSGLVTANITDSEALARWQPAIATVDSYVISYTGEKVPEITRTVSGNTVEYALTDLE PATEYTLRIFAEKGPQKSSTITAKFTTDLDSPRDLTATEVQSETALLTWRPPRASVTGYL LVYESVDGTVKEVIVGPDTTSYSLADLSPSTHYTAKIQALNGPLRSNMIQTIFTTIGLLYP FPKDCSQAMLNGDTTSGLYTIYLNGDKAQALEVFCDMTSDGGGWIVFLRRKNGRENF YQNWKAYAAGFGDRREEFWLGLDNLNKITAQGQYELRVDLRDHGETAFAVYDKFSV GDAKTRYKLKVEGYSGTAGDSMAYHNGRSFSTFDKDTDSAITNCALSYKGAFWYRNC HRVNLMGRYGDNNHSQGVNWFHWKGHEHSIQFAEMKLRPSNFRNLEGRRKRA corresponding to amino acids 1617-2201 of TENA_HUMAN_V1 (SEQ ID NO: 1011), which also corresponds to amino acids 1071-1655 of HUMTEN_PEA_(—)1_P15 (SEQ ID NO: 942), wherein said first amino acid sequence and second amino acid sequence are contiguous and in a sequential order.

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for an edge portion of HUMTEN_PEA_(—)1_P15 (SEQ ID NO: 942), comprising a polypeptide having a length “n”, wherein n is at least about 10 amino acids in length, optionally at least about 20 amino acids in length, preferably at least about 30 amino acids in length, more preferably at least about 40 amino acids in length and most preferably at least about 50 amino acids in length, wherein at least two amino acids comprise ST, having a structure as follows: a sequence starting from any of amino acid numbers 1070-x to 1070; and ending at any of amino acid numbers 1071+((n−2)−x), in which x varies from 0 to n−2.

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for HUMTEN_PEA_(—)1_P16 (SEQ ID NO: 943), comprising a first amino acid sequence being at least 90% homologous to MGAMTQLLAGVFLAFLALATEGGVLKKVIRHKRQSGVNATLPEENQPVVFNHVYNIK LPVGSQCSVDLESASGEKDLAPPSEPSESFQEHTVDGENQIVFTHRINIPRRACGCAAAP DVKELLSRLEELENLVSSLREQCTAGAGCCLQPATGRLDTRPFCSGRGNFSTEGCGCVC EPGWKGPNCSEPECPGNCHLRGRCIDGQCICDDGFTGEDCSQLACPSDCNDQGKCVNG VCICFEGYAGADCSREICPVPCSEEHGTCVDGLCVCHDGFAGDDCNKPLCLNNCYNRG RCVENECVCDEGFTGEDCSELICPNDCFDRGRCINGTCYCEEGFTGEDCGKPTCPHACH TQGRCEEGQCVCDEGFAGVDCSEKRCPADCHNRGRCVDGRCECDDGFTGADCGELKC PNGCSGHGRCVNGQCVCDEGYTGEDCSQLRCPNDCHSRGRCVEGKCVCEQGFKGYDC SDMSCPNDCHQHGRCVNGMCVCDDGYTGEDCRDRQCPRDCSNRGLCVDGQCVCEDG FTGPDCAELSCPNDCHGRGRCVNGQCVCHEGFMGKDCKEQRCPSDCHGQGRCVDGQ CICHEGFTGLDCGQHSCPSDCNNLGQCVSGRCICNEGYSGEDCSEVSPPKDLVVTEVTE ETVNLAWDNEMRVTEYLVVYTPTHEGGLEMQFRVPGDQTSTIIQELEPGVEYFIRVFAI LENKKSIPVSARVATYLPAPEGLKFKSIKETSVEVEWDPLDIAFETWEIIFRNMNKEDEG EITKSLRRPETSYRQTGLAPGQEYEISLHIVKNNTRGPGLKRVTTTRLDAPSQIEVKDVT DTTALITWFKPLAEIDGIELTYGIKDVPGDRTTIDLTEDENQYSIGNLKPDTEYEVSLISRR GDMSSNPAKETFTTGLDAPRNLRRVSQTDNSITLEWRNGKAAIDSYRIKYAPISGGDHA EVDVPKSQQATTKTTLTGLRPGTEYGIGVSAVKEDKESNPATINAATELDTPKDLQVSE TAETSLTLLWKTPLAKFDRYRLNYSLPTGQWVGVQLPRNTTSYVLRGLEPGQEYNVLL TAEKGRHKSKPARVKAS corresponding to amino acids 1-1070 of TENA_HUMAN_V1 (SEQ ID NO: 1011), which also corresponds to amino acids 1-1070 of HUMTEN_PEA_(—)1_P16 (SEQ ID NO: 943), and a second amino acid sequence being at least 90% homologous to TAMGSPKEVIFSDITENSATVSWRAPTAQVESFRITYVPITGGTPSMVTVDGTKTQTRLV KLIPGVEYLVSIIAMKGFEESEPVSGSFTTALDGPSGLVTANITDSEALARWQPAIATVDS YVISYTGEKVPEITRTVSGNTVEYALTDLEPATEYTLRIFAEKGPQKSSTITAKFTTDLDS PRDLTATEVQSETALLTWRPPRASVTGYLLVYESVDGTVKEVIVGPDTTSYSLADLSPS THYTAKIQALNGPLRSNMIQTIFTTIGLLYPFPKDCSQAMLNGDTTSGLYTIYLNGDKAQ ALEVFCDMTSDGGGWIVFLRRKNGRENFYQNWKAYAAGFGDRREEFWLGLDNLNKIT AQGQYELRVDLRDHGETAFAVYDKFSVGDAKTRYKLKVEGYSGTAGDSMAYHNGRS FSTFDKDTDSAITNCALSYKGAFWYRNCHRVNLMGRYGDNNHSQGVNWFHWKGHEH SIQFAEMKLRPSNFRNLEGRRKRA corresponding to amino acids 1708-2201 of TENA_HUMAN_V1 (SEQ ID NO: 1011), which also corresponds to amino acids 1071-1564 of HUMTEN_PEA_(—)1_P16 (SEQ ID NO: 943), wherein said first amino acid sequence and second amino acid sequence are contiguous and in a sequential order.

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for an edge portion of HUMTEN_PEA_(—)1_P16 (SEQ ID NO: 943), comprising a polypeptide having a length “n”, wherein n is at least about 10 amino acids in length, optionally at least about 20 amino acids in length, preferably at least about 30 amino acids in length, more preferably at least about 40 amino acids in length and most preferably at least about 50 amino acids in length, wherein at least two amino acids comprise ST, having a structure as follows: a sequence starting from any of amino acid numbers 1070-x to 1070; and ending at any of amino acid numbers 1071+((n−2)−x), in which x varies from 0 to n−2.

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for HUMTEN_PEA_(—)1_P17 (SEQ ID NO: 944), comprising a first amino acid sequence being at least 90% homologous to MGAMTQLLAGVFLAFLALATEGGVLKKVIRHKRQSGVNATLPEENQPVVFNHVYNIK LPVGSQCSVDLESASGEKDLAPPSEPSESFQEHTVDGENQIVFTHRINIPRRACGCAAAP DVKELLSRLEELENLVSSLREQCTAGAGCCLQPATGRLDTRPFCSGRGNFSTEGCGCVC EPGWKGPNCSEPECPGNCHLRGRCIDGQCICDDGFTGEDCSQLACPSDCNDQGKCVNG VCICFEGYAGADCSREICPVPCSEEHGTCVDGLCVCHDGFAGDDCNKPLCLNNCYNRG RCVENECVCDEGFTGEDCSELICPNDCFDRGRCINGTCYCEEGFTGEDCGKPTCPHACH TQGRCEEGQCVCDEGFAGVDCSEKRCPADCHNRGRCVDGRCECDDGFTGADCGELKC PNGCSGHGRCVNGQCVCDEGYTGEDCSQLRCPNDCHSRGRCVEGKCVCEQGFKGYDC SDMSCPNDCHQHGRCVNGMCVCDDGYTGEDCRDRQCPRDCSNRGLCVDGQCVCEDG FTGPDCAELSCPNDCHGRGRCVNGQCVCHEGFMGKDCKEQRCPSDCHGQGRCVDGQ CICHEGFTGLDCGQHSCPSDCNNLGQCVSGRCICNEGYSGEDCSEVSPPKDLVVTEVTE ETVNLAWDNEMRVTEYLVVYTPTHEGGLEMQFRVPGDQTSTIIQELEPGVEYFIRVFAI LENKKSIPVSARVATYLPAPEGLKFKSIKETSVEVEWDPLDIAFETWEIIFRNMNKEDEG EITKSLRRPETSYRQTGLAPGQEYEISLHIVKNNTRGPGLKRVTTTRLDAPSQIEVKDVT DTTALITWFKPLAEIDGIELTYGIKDVPGDRTTIDLTEDENQYSIGNLKPDTEYEVSLISRR GDMSSNPAKETFTTGLDAPRNLRRVSQTDNSITLEWRNGKAAIDSYRIKYAPISGGDHA EVDVPKSQQATTKTTLTGLRPGTEYGIGVSAVKEDKESNPATINAATELDTPKDLQVSE TAETSLTLLWKTPLAKFDRYRLNYSLPTGQWVGVQLPRNTTSYVLRGLEPGQEYNVLL TAEKGRHKSKPARVKASTEQAPELENLTVTEVGWDGLRLNWTAADQAYEHFIIQVQE ANKVEAARNLTVPGSLRAVDIPGLKAATPYTVSIYGVIQGYRTPVLSAEASTGETPNLG EVVVAEVGWDALKLNWTAPEGAYEYFFIQVQEADTVEAAQNLTVPGGLRSTDLPGLK AATHYTITIRGVTQDFSTTPLSVEVLTEEVPDMGNLTVTEVSWDALRLNWTTPDGTYD QFTIQVQEADQVEEAHNLTVPGSLRSMEIPGLRAGTPYTVTLHGEVRGHSTRPLAVEVV TEDLPQLGDLAVSEVGWDGLRLNWTAADNAYEHFVIQVQEVNKVEAAQNLTLPGSLR AVDIPGLEAATPYRVSIYGVIRGYRTPVLSAEASTAKEPEIGNLNVSDITPESFNLSWMA TDGIFETFTIEIIDSNRLLETVEYNISGAERTAHISGLPPSTDFIVYLSGLAPSIRTKTISATA TTEALPLLENLTISDINPYGFTVSWMASENAFDSFLVTVVDSGKLLDPQEFTLSGTQRKL ELRGLITGIGYEVMVSGFTQGHQTKPLRAEIVTEAEPEVDNLLVSDATPDGFRLSWTAD EGVFDNFVLKIRDTKKQSEPLEITLLAPERTRDLTGLREATEYEIELYGISKGRRSQTVSA IATTAMGSPKEVIFSDITENSATVSWRAPTAQVESFRITYVPITGGTPSMVTVDGTKTQT RLVKLIPGVEYLVSIIAMKGFEESEPVSGSFTTALDGPSGLVTANITDSEALARWQPAIAT VDSYVISYTGEKVPEITRTVSGNTVEYALTDLEPATEYTLRIFAEKGPQKSSTITAKFTTD LDSPRDLTATEVQSETALLTWRPPRASVTGYLLVYESVDGTVKEVIVGPDTTSYSLADL SPSTHYTAKIQALNGPLRSNMIQTIFTTIGLLYPFPKDCSQAMLNGDTTSGLYTIYLNGD KAQALEVFCDMTSDGGGWIV corresponding to amino acids 1-2025 of TENA_HUMAN_V1 (SEQ ID NO: 1011), which also corresponds to amino acids 1-2025 of HUMTEN_PEA_(—)1_P17 (SEQ ID NO: 944), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence TPWPTTMADPSPPLTRTQIQPSPTVLCPTKGLSGTGTVTVST (SEQ ID NO: 1101) corresponding to amino acids 2026-2067 of HUMTEN_PEA_(—)1_P17 (SEQ ID NO: 944), wherein said first amino acid sequence and second amino acid sequence are contiguous and in a sequential order.

According to preferred embodiments of the present invention, there is provided an isolated polypeptide encoding for a tail of HUMTEN_PEA_(—)1_P17 (SEQ ID NO: 944), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence TPWPTTMADPSPPLTRTQIQPSPTVLCPTKGLSGTGTVTVST (SEQ ID NO: 1101) in HUMTEN_PEA_(—)1_P17 (SEQ ID NO: 944).

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for HUMTEN_PEA_(—)1_P20 (SEQ ID NO: 945), comprising a first amino acid sequence being at least 90% homologous to MGAMTQLLAGVFLAFLALATEGGVLKKVIRHKRQSGVNATLPEENQPVVFNHVYNIK LPVGSQCSVDLESASGEKDLAPPSEPSESFQEHTVDGENQIVFTHRINIPRRACGCAAAP DVKELLSRLEELENLVSSLREQCTAGAGCCLQPATGRLDTRPFCSGRGNFSTEGCGCVC EPGWKGPNCSEPECPGNCHLRGRCIDGQCICDDGFTGEDCSQLACPSDCNDQGKCVNG VCICFEGYAGADCSREICPVPCSEEHGTCVDGLCVCHDGFAGDDCNKPLCLNNCYNRG RCVENECVCDEGFTGEDCSELICPNDCFDRGRCINGTCYCEEGFTGEDCGKPTCPHACH TQGRCEEGQCVCDEGFAGVDCSEKRCPADCHNRGRCVDGRCECDDGFTGADCGELKC PNGCSGHGRCVNGQCVCDEGYTGEDCSQLRCPNDCHSRGRCVEGKCVCEQGFKGYDC SDMSCPNDCHQHGRCVNGMCVCDDGYTGEDCRDRQCPRDCSNRGLCVDGQCVCEDG FTGPDCAELSCPNDCHGRGRCVNGQCVCHEGFMGKDCKEQRCPSDCHGQGRCVDGQ CICHEGFTGLDCGQHSCPSDCNNLGQCVSGRCICNEGYSGEDCSEVSPPKDLVVTEVTE ETVNLAWDNEMRVTEYLVVYTPTHEGGLEMQFRVPGDQTSTIIQELEPGVEYFIRVFAI LENKKSIPVSARVATYLPAPEGLKFKSIKETSVEVEWDPLDIAFETWEIIFRNMNKEDEG EITKSLRRPETSYRQTGLAPGQEYEISLHIVKNNTRGPGLKRVTTTRLDAPSQIEVKDVT DTTALITWFKPLAEIDGIELTYGIKDVPGDRTTIDLTEDENQYSIGNLKPDTEYEVSLISRR GDMSSNPAKETFTTGLDAPRNLRRVSQTDNSITLEWRNGKAAIDSYRIKYAPISGGDHA EVDVPKSQQATTKTTLTGLRPGTEYGIGVSAVKEDKESNPATINAATELDTPKDLQVSE TAETSLTLLWKTPLAKFDRYRLNYSLPTGQWVGVQLPRNTTSYVLRGLEPGQEYNVLL TAEKGRHKSKPARVKASTEQAPELENLTVTEVGWDGLRLNWTAADQAYEHFIIQVQE ANKVEAARNLTVPGSLRAVDIPGLKAATPYTVSIYGVIQGYRTPVLSAEASTGETPNLG EVVVAEVGWDALKLNWTAPEGAYEYFFIQVQEADTVEAAQNLTVPGGLRSTDLPGLK AATHYTITIRGVTQDFSTTPLSVEVLTEEVPDMGNLTVTEVSWDALRLNWTTPDGTYD QFTIQVQEADQVEEAHNLTVPGSLRSMEIPGLRAGTPYTVTLHGEVRGHSTRPLAVEVV TEDLPQLGDLAVSEVGWDGLRLNWTAADNAYEHFVIQVQEVNKVEAAQNLTLPGSLR AVDIPGLEAATPYRVSIYGVIRGYRTPVLSAEASTAKEPEIGNLNVSDITPESFNLSWMA TDGIFETFTIEIIDSNRLLETVEYNISGAERTAHISGLPPSTDFIVYLSGLAPSIRTKTISATA TTEALPLLENLTISDINPYGFTVSWMASENAFDSFLVTVVDSGKLLDPQEFTLSGTQRKL ELRGLITGIGYEVMVSGFTQGHQTKPLRAEIVTEAEPEVDNLLVSDATPDGFRLSWTAD EGVFDNFVLKIRDTKKQSEPLEITLLAPERTRDLTGLREATEYEIELYGISKGRRSQTVSA IATTAMGSPKEVIFSDITENSATVSWRAPTAQVESFRITYVPITGGTPSMVTVDGTKTQT RLVKLIPGVEYLVSIIAMKGFEESEPVSGSFTTALDGPSGLVTANITDSEALARWQPAIAT VDSYVISYTGEKVPEITRTVSGNTVEYALTDLEPATEYTLRIFAEKGPQKSSTITAKFTTD LDSPRDLTATEVQSETALLTWRPPRASVTGYLLVYESVDGTVKEVIVGPDTTSYSLADL SPSTHYTAKIQALNGPLRSNMIQTIFTTIGLLYPFPKDCSQAMLNGDTTSGLYTIYLNGD KAQALEVFCDMTSDGGGWIVFLRRKNGRENFYQNWKAYAAGFGDRREEFWLG corresponding to amino acids 1-2057 of TENA_HUMAN_V1 (SEQ ID NO: 1011), which also corresponds to amino acids 1-2057 of HUMTEN_PEA_(—)1_P20 (SEQ ID NO: 945), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence NAALHVYI (SEQ ID NO: 1102) corresponding to amino acids 2058-2065 of HUMTEN_PEA_(—)1_P20 (SEQ ID NO: 945), wherein said first amino acid sequence and second amino acid sequence are contiguous and in a sequential order.

According to preferred embodiments of the present invention, there is provided an isolated polypeptide encoding for a tail of HUMTEN_PEA_(—)1_P20 (SEQ ID NO: 945), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence NAALHVYI (SEQ ID NO: 1102) in HUMTEN_PEA_(—)1_P20 (SEQ ID NO: 945).

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for HUMTEN_PEA_(—)1_P26 (SEQ ID NO: 946), comprising a first amino acid sequence being at least 90% homologous to MGAMTQLLAGVFLAFLALATEGGVLKKVIRHKRQSGVNATLPEENQPVVFNHVYNIK LPVGSQCSVDLESASGEKDLAPPSEPSESFQEHTVDGENQIVFTHRINIPRRACGCAAAP DVKELLSRLEELENLVSSLREQCTAGAGCCLQPATGRLDTRPFCSGRGNFSTEGCGCVC EPGWKGPNCSEPECPGNCHLRGRCIDGQCICDDGFTGEDCSQLACPSDCNDQGKCVNG VCICFEGYAGADCSREICPVPCSEEHGTCVDGLCVCHDGFAGDDCNKPLCLNNCYNRG RCVENECVCDEGFTGEDCSELICPNDCFDRGRCINGTCYCEEGFTGEDCGKPTCPHACH TQGRCEEGQCVCDEGFAGVDCSEKRCPADCHNRGRCVDGRCECDDGFTGADCGELKC PNGCSGHGRCVNGQCVCDEGYTGEDCSQLRCPNDCHSRGRCVEGKCVCEQGFKGYDC SDMSCPNDCHQHGRCVNGMCVCDDGYTGEDCRDRQCPRDCSNRGLCVDGQCVCEDG FTGPDCAELSCPNDCHGRGRCVNGQCVCHEGFMGKDCKEQRCPSDCHGQGRCVDGQ CICHEGFTGLDCGQHSCPSDCNNLGQCVSGRCICNEGYSGEDCSEVSPPKDLVVTEVTE ETVNLAWDNEMRVTEYLVVYTPTHEGGLEMQFRVPGDQTSTIIQELEPGVEYFIRVFAI LENKKSIPVSARVATYLPAPEGLKFKSIKETSVEVEWDPLDIAFETWEIIFRNMNKEDEG EITKSLRRPETSYRQTGLAPGQEYEISLHIVKNNTRGPGLKRVTTTRLDAPSQIEVKDVT DTTALITWFKPLAEIDGIELTYGIKDVPGDRTTIDLTEDENQYSIGNLKPDTEYEVSLISRR GDMSSNPAKETFTTGLDAPRNLRRVSQTDNSITLEWRNGKAAIDSYRIKYAPISGGDHA EVDVPKSQQATTKTTLTGLRPGTEYGIGVSAVKEDKESNPATINAATELDTPKDLQVSE TAETSLTLLWKTPLAKFDRYRLNYSLPTGQWVGVQLPRNTTSYVLRGLEPGQEYNVLL TAEKGRHKSKPARVKASTEQAPELENLTVTEVGWDGLRLNWTAADQAYEHFIIQVQE ANKVEAARNLTVPGSLRAVDIPGLKAATPYTVSIYGVIQGYRTPVLSAEASTGETPNLG EVVVAEVGWDALKLNWTAPEGAYEYFFIQVQEADTVEAAQNLTVPGGLRSTDLPGLK AATHYTITIRGVTQDFSTTPLSVEVLTEEVPDMGNLTVTEVSWDALRLNWTTPDGTYD QFTIQVQEADQVEEAHNLTVPGSLRSMEIPGLRAGTPYTVTLHGEVRGHSTRPLAVEVV TEDLPQLGDLAVSEVGWDGLRLNWTAADNAYEHFVIQVQEVNKVEAAQNLTLPGSLR AVDIPGLEAATPYRVSIYGVIRGYRTPVLSAEASTAKEPEIGNLNVSDITPESFNLSWMA TDGIFETFTIEIIDSNRLLETVEYNISGAERTAHISGLPPSTDFIVYLSGLAPSIRTKTISATA TTEALPLLENLTISDINPYGFTVSWMASENAFDSFLVTVVDSGKLLDPQEFTLSGTQRKL ELRGLITGIGYEVMVSGFTQGHQTKPLRAEIVTEAEPEVDNLLVSDATPDGFRLSWTAD EGVFDNFVLKIRDTKKQSEPLEITLLAPERTRDLTGLREATEYEIELYGISKGRRSQTVSA IATT corresponding to amino acids 1-1708 of TENA_HUMAN_V1 (SEQ ID NO: 1011), which also corresponds to amino acids 1-1708 of HUMTEN_PEA_(—)1_P26 (SEQ ID NO: 946), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence GTVNKQERTEKSHDSGVFFSQG (SEQ ID NO: 1103) corresponding to amino acids 1709-1730 of HUMTEN_PEA_(—)1_P26 (SEQ ID NO: 946), wherein said first amino acid sequence and second amino acid sequence are contiguous and in a sequential order.

According to preferred embodiments of the present invention, there is provided an isolated polypeptide encoding for a tail of HUMTEN_PEA_(—)1_P26 (SEQ ID NO: 946), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence GTVNKQERTEKSHDSGVFFSQG (SEQ ID NO: 1103) in HUMTEN_PEA_(—)1_P26 (SEQ ID NO: 946).

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for HUMTEN_PEA_(—)1_P27 (SEQ ID NO: 947), comprising a first amino acid sequence being at least 90% homologous to MGAMTQLLAGVFLAFLALATEGGVLKKVIRHKRQSGVNATLPEENQPVVFNHVYNIK LPVGSQCSVDLESASGEKDLAPPSEPSESFQEHTVDGENQIVFTHRRRACGCAAAP DVKELLSRLEELENLVSSLREQCTAGAGCCLQPATGRLDTRPFCSGRGNFSTEGCGCVC EPGWKGPNCSEPECPGNCHLRGRCIDGQCICDDGFTGEDCSQLACPSDCNDQGKCVNG VCICFEGYAGADCSREICPVPCSEEHGTCVDGLCVCHDGFAGDDCNKPLCLNNCYNRG RCVENECVCDEGFTGEDCSELICPNDCFDRGRCINGTCYCEEGFTGEDCGKPTCPHACH TQGRCEEGQCVCDEGFAGVDCSEKRCPADCHNRGRCVDGRCECDDGFTGADCGELKC PNGCSGHGRCVNGQCVCDEGYTGEDCSQLRCPNDCHSRGRCVEGKCVCEQGFKGYDC SDMSCPNDCHQHGRCVNGMCVCDDGYTGEDCRDRQCPRDCSNRGLCVDGQCVCEDG FTGPDCAELSCPNDCHGRGRCVNGQCVCHEGFMGKDCKEQRCPSDCHGQGRCVDGQ CICHEGFTGLDCGQHSCPSDCNNLGQCVSGRCICNEGYSGEDCSEVSPPKDLVVTEVTE ETVNLAWDNEMRVTEYLVVYTPTHEGGLEMQFRVPGDQTSTIIQELEPGVEYFIRVFAI LENKKSIPVSARVATYLPAPEGLKFKSIKETSVEVEWDPLDIAFETWEIIFRNMNKEDEG EITKSLRRPETSYRQTGLAPGQEYEISLHIVKNNTRGPGLKRVTTTRLDAPSQIEVKDVT DTTALITWFKPLAEIDGIELTYGIKDVPGDRTTIDLTEDENQYSIGNLKPDTEYEVSLISRR GDMSSNPAKETFTTGLDAPRNLRRVSQTDNSITLEWRNGKAAIDSYRIKYAPISGGDHA EVDVPKSQQATTKTTLTGLRPGTEYGIGVSAVKEDKESNPATINAATELDTPKDLQVSE TAETSLTLLWKTPLAKFDRYRLNYSLPTGQWVGVQLPRNTTSYVLRGLEPGQEYNVLL TAEKGRHKSKPARVKASTEQAPELENLTVTEVGWDGLRLNWTAADQAYEHFIIQVQE ANKVEAARNLTVPGSLRAVDIPGLKAATPYTVSIYGVIQGYRTPVLSAEASTGETPNLG EVVVAEVGWDALKLNWTAPEGAYEYFFIQVQEADTVEAAQNLTVPGGLRSTDLPGLK AATHYTITIRGVTQDFSTTPLSVEVLTEEVPDMGNLTVTEVSWDALRLNWTTPDGTYD QFTIQVQEADQVEEAHNLTVPGSLRSMEIPGLRAGTPYTVTLHGEVRGHSTRPLAVEVV T corresponding to amino acids 1-1344 of TENA_HUMAN_V1 (SEQ ID NO: 1011), which also corresponds to amino acids 1-1344 of HUMTEN_PEA_(—)1_P27 (SEQ ID NO: 947), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence GI corresponding to amino acids 1345-1346 of HUMTEN_PEA_(—)1_P27 (SEQ ID NO: 947), wherein said first amino acid sequence and second amino acid sequence are contiguous and in a sequential order.

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for HUMTEN_PEA_(—)1_P28 (SEQ ID NO: 948), comprising a first amino acid sequence being at least 90% homologous to MGAMTQLLAGVFLAFLALATEGGVLKKVIRHKRQSGVNATLPEENQPVVFNHVYNIK LPVGSQCSVDLESASGEKDLAPPSEPSESFQEHTVDGENQIVFTHRINIPRRACGCAAAP DVKELLSRLEELENLVSSLREQCTAGAGCCLQPATGRLDTRPFCSGRGNFSTEGCGCVC EPGWKGPNCSEPECPGNCHLRGRCIDGQCICDDGFTGEDCSQLACPSDCNDQGKCVNG VCICFEGYAGADCSREICPVPCSEEHGTCVDGLCVCHDGFAGDDCNKPLCLNNCYNRG RCVENECVCDEGFTGEDCSELICPNDCFDRGRCINGTCYCEEGFTGEDCGKPTCPHACH TQGRCEEGQCVCDEGFAGVDCSEKRCPADCHNRGRCVDGRCECDDGFTGADCGELKC PNGCSGHGRCVNGQCVCDEGYTGEDCSQLRCPNDCHSRGRCVEGKCVCEQGFKGYDC SDMSCPNDCHQHGRCVNGMCVCDDGYTGEDCRDRQCPRDCSNRGLCVDGQCVCEDG FTGPDCAELSCPNDCHGRGRCVNGQCVCHEGFMGKDCKEQRCPSDCHGQGRCVDGQ CICHEGFTGLDCGQHSCPSDCNNLGQCVSGRCICNEGYSGEDCSEVSPPKDLVVTEVTE ETVNLAWDNEMRVTEYLVVYTPTHEGGLEMQFRVPGDQTSTIIQELEPGVEYFIRVFAI LENKKSIPVSARVATYLPAPEGLKFKSIKETSVEVEWDPLDIAFETWEIIFRNMNKEDEG EITKSLRRPETSYRQTGLAPGQEYEISLHIVKNNTRGPGLKRVTTTRLDAPSQIEVKDVT DTTALITWFKPLAEIDGIELTYGIKDVPGDRTTIDLTEDENQYSIGNLKPDTEYEVSLISRR GDMSSNPAKETFTTGLDAPRNLRRVSQTDNSITLEWRNGKAAIDSYRIKYAPISGGDHA EVDVPKSQQATTKTTLTGLRPGTEYGIGVSAVKEDKESNPATINAATELDTPKDLQVSE TAETSLTLLWKTPLAKFDRYRLNYSLPTGQWVGVQLPRNTTSYVLRGLEPGQEYNVLL TAEKGRHKSKPARVKASTEQAPELENLTVTEVGWDGLRLNWTAADQAYEHFIIQVQE ANKVEAARNLTVPGSLRAVDIPGLKAATPYTVSIYGVIQGYRTPVLSAEASTGETPNLG EVVVAEVGWDALKLNWTAPEGAYEYFFIQVQEADTVEAAQNLTVPGGLRSTDLPGLK AATHYTITIRGVTQDFSTTPLSVEVLT corresponding to amino acids 1-1253 of TENA_HUMAN_V1 (SEQ ID NO: 1011), which also corresponds to amino acids 1-1253 of HUMTEN_PEA_(—)1_P28 (SEQ ID NO: 948), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence GILDEFTNSLPPLCLCSGGIKALSCFKLGSAPTTLGKYQ (SEQ ID NO: 1104) corresponding to amino acids 1254-1292 of HUMTEN_PEA_(—)1_P28 (SEQ ID NO: 948), wherein said first amino acid sequence and second amino acid sequence are contiguous and in a sequential order.

According to preferred embodiments of the present invention, there is provided an isolated polypeptide encoding for a tail of HUMTEN_PEA_(—)1_P28 (SEQ ID NO: 948), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence GILDEFTNSLPPLCLCSGGIKALSCFKLGSAPTTLGKYQ (SEQ ID NO: 1104) in HUMTEN_PEA_(—)1_P28 (SEQ ID NO: 948).

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for HUMTEN_PEA_(—)1_P29 (SEQ ID NO: 949), comprising a first amino acid sequence being at least 90% homologous to MGAMTQLLAGVFLAFLALATEGGVLKKVIRHKRQSGVNATLPEENQPVVFNHVYNIK LPVGSQCSVDLESASGEKDLAPPSEPSESFQEHTVDGENQIVFTHRINIPRRACGCAAAP DVKELLSRLEELENLVSSLREQCTAGAGCCLQPATGRLDTRPFCSGRGNFSTEGCGCVC EPGWKGPNCSEPECPGNCHLRGRCIDGQCICDDGFTGEDCSQLACPSDCNDQGKCVNG VCICFEGYAGADCSREICPVPCSEEHGTCVDGLCVCHDGFAGDDCNKPLCLNNCYNRG RCVENECVCDEGFTGEDCSELICPNDCFDRGRCINGTCYCEEGFTGEDCGKPTCPHACH TQGRCEEGQCVCDEGFAGVDCSEKRCPADCHNRGRCVDGRCECDDGFTGADCGELKC PNGCSGHGRCVNGQCVCDEGYTGEDCSQLRCPNDCHSRGRCVEGKCVCEQGFKGYDC SDMSCPNDCHQHGRCVNGMCVCDDGYTGEDCRDRQCPRDCSNRGLCVDGQCVCEDG FTGPDCAELSCPNDCHGRGRCVNGQCVCHEGFMGKDCKEQRCPSDCHGQGRCVDGQ CICHEGFTGLDCGQHSCPSDCNNLGQCVSGRCICNEGYSGEDCSEVSPPKDLVVTEVTE ETVNLAWDNEMRVTEYLVVYTPTHEGGLEMQFRVPGDQTSTIIQELEPGVEYFIRVFAI LENKKSIPVSARVATYLPAPEGLKFKSIKETSVEVEWDPLDIAFETWEIIFRNMNKEDEG EITKSLRRPETSYRQTGLAPGQEYEISLHIVKNNTRGPGLKRVTTTRLDAPSQIEVKDVT DTTALITWFKPLAEIDGIELTYGIKDVPGDRTTIDLTEDENQYSIGNLKPDTEYEVSLISRR GDMSSNPAKETFTTGLDAPRNLRRVSQTDNSITLEWRNGKAAIDSYRIKYAPISGGDHA EVDVPKSQQATTKTTLTGLRPGTEYGIGVSAVKEDKESNPATINAATELDTPKDLQVSE TAETSLTLLWKTPLAKFDRYRLNYSLPTGQWVGVQLPRNTTSYVLRGLEPGQEYNVLL TAEKGRHKSKPARVKAST corresponding to amino acids 1-1071 of TENA_HUMAN_V1 (SEQ ID NO: 1011), which also corresponds to amino acids 1-1071 of HUMTEN_PEA_(—)1_P29 (SEQ ID NO: 949), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence GESALSFLQTLG (SEQ ID NO: 1105) corresponding to amino acids 1072-1083 of HUMTEN_PEA_(—)1_P29 (SEQ ID NO: 949), wherein said first amino acid sequence and second amino acid sequence are contiguous and in a sequential order.

According to preferred embodiments of the present invention, there is provided an isolated polypeptide encoding for a tail of HUMTEN_PEA_(—)1_P29 (SEQ ID NO: 949), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence GESALSFLQTLG (SEQ ID NO: 1105) in HUMTEN_PEA_(—)1_P29 (SEQ ID NO: 949).

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for HUMTEN_PEA_(—)1_P30 (SEQ ID NO: 950), comprising a first amino acid sequence being at least 90% homologous to MGAMTQLLAGVFLAFLALATEGGVLKKVIRHKRQSGVNATLPEENQPVVFNHVYNIK LPVGSQCSVDLESASGEKDLAPPSEPSESFQEHTVDGENQIVFTHRINIPRRACGCAAAP DVKELLSRLEELENLVSSLREQCTAGAGCCLQPATGRLDTRPFCSGRGNFSTEGCGCVC EPGWKGPNCSEPECPGNCHLRGRCIDGQCICDDGFTGEDCSQLACPSDCNDQGKCVNG VCICFEGYAGADCSREICPVPCSEEHGTCVDGLCVCHDGFAGDDCNKPLCLNNCYNRG RCVENECVCDEGFTGEDCSELICPNDCFDRGRCINGTCYCEEGFTGEDCGKPTCPHACH TQGRCEEGQCVCDEGFAGVDCSEKRCPADCHNRGRCVDGRCECDDGFTGADCGELKC PNGCSGHGRCVNGQCVCDEGYTGEDCSQLRCPNDCHSRGRCVEGKCVCEQGFKGYDC SDMSCPNDCHQHGRCVNGMCVCDDGYTGEDCRDRQCPRDCSNRGLCVDGQCVCEDG FTGPDCAELSCPNDCHGRGRCVNGQCVCHEGFMGKDCKEQRCPSDCHGQGRCVDGQ CICHEGFTGLDCGQHSCPSDCNNLGQCVSGRCICNEGYSGEDCSEVSPPKDLVVTEVTE ETVNLAWDNEMRVTEYLVVYTPTHEGGLEMQFRVPGDQTSTIIQELEPGVEYFIRVFAI LENKKSIPVSARVATYLPAPEGLKFKSIKETSVEVEWDPLDIAFETWEIIFRNMNKEDEG EITKSLRRPETSYRQTGLAPGQEYEISLHIVKNNTRGPGLKRVTTTRLDAPSQIEVKDVT DTTALITWFKPLAEIDGIELTYGIKDVPGDRTTIDLTEDENQYSIGNLKPDTEYEVSLISRR GDMSSNPAKETFTTGLDAPRNLRRVSQTDNSITLEWRNGKAAIDSYRIKYAPISGGDHA EVDVPKSQQATTKTTLTG corresponding to amino acids 1-954 of TENA_HUMAN_V1 (SEQ ID NO: 1011), which also corresponds to amino acids 1-954 of HUMTEN_PEA_(—)1_P30 (SEQ ID NO: 950), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence ELCISASLSQPALEGP (SEQ ID NO: 1106) corresponding to amino acids 955-970 of HUMTEN_PEA_(—)1_P30 (SEQ ID NO: 950), wherein said first amino acid sequence and second amino acid sequence are contiguous and in a sequential order.

According to preferred embodiments of the present invention, there is provided an isolated polypeptide encoding for a tail of HUMTEN_PEA_(—)1_P30 (SEQ ID NO: 950), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95%

homologous to the sequence ELCISASLSQPALEGP (SEQ ID NO: 1106) in HUMTEN_PEA_(—)1_P30 (SEQ ID NO: 950).

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for HUMTEN_PEA_(—)1_P31 (SEQ ID NO: 951), comprising a first amino acid sequence being at least 90% homologous to MGAMTQLLAGVFLAFLALATEGGVLKKVIRHKRQSGVNATLPEENQPVVFNHVYNIK LPVGSQCSVDLESASGEKDLAPPSEPSESFQEHTVDGENQIVFTHRINIPRRACGCAAAP DVKELLSRLEELENLVSSLREQCTAGAGCCLQPATGRLDTRPFCSGRGNFSTEGCGCVC EPGWKGPNCSEPECPGNCHLRGRCIDGQCICDDGFTGEDCSQLACPSDCNDQGKCVNG VCICFEGYAGADCSREICPVPCSEEHGTCVDGLCVCHDGFAGDDCNKPLCLNNCYNRG RCVENECVCDEGFTGEDCSELICPNDCFDRGRCINGTCYCEEGFTGEDCGKPTCPHACH TQGRCEEGQCVCDEGFAGVDCSEKRCPADCHNRGRCVDGRCECDDGFTGADCGELKC PNGCSGHGRCVNGQCVCDEGYTGEDCSQLRCPNDCHSRGRCVEGKCVCEQGFKGYDC SDMSCPNDCHQHGRCVNGMCVCDDGYTGEDCRDRQCPRDCSNRGLCVDGQCVCEDG FTGPDCAELSCPNDCHGRGRCVNGQCVCHEGFMGKDCKEQRCPSDCHGQGRCVDGQ CICHEGFTGLDCGQHSCPSDCNNLGQCVSGRCICNEGYSGEDCSEVSPPKDLVVTEVTE ETVNLAWDNEMRVTEYLVVYTPTHEGGLEMQFRVPGDQTSTIIQELEPGVEYFIRVFAI LENKKSIPVSARVATYLPAPEGLKFKSIKETSVEVEWDPLDIAFETWEIIFRNMNKEDEG EITKSLRRPETSYRQTGLAPGQEYEISLHIVKNNTRGPGLKRVTTTR corresponding to amino acids 1-802 of TENA_HUMAN_V1 (SEQ ID NO: 1011), which also corresponds to amino acids 1-802 of HUMTEN_PEA_(—)1_P31 (SEQ ID NO: 951), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence EYHL (SEQ ID NO: 1107) corresponding to amino acids 803-806 of HUMTEN_PEA_(—)1_P31 (SEQ ID NO: 951), wherein said first amino acid sequence and second amino acid sequence are contiguous and in a sequential order.

According to preferred embodiments of the present invention, there is provided an isolated polypeptide encoding for a tail of HUMTEN_PEA_(—)1_P31 (SEQ ID NO: 951), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence EYHL (SEQ ID NO: 1107) in HUMTEN_PEA_(—)1_P31 (SEQ ID NO: 951).

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for HUMTEN_PEA_(—)1_P32 (SEQ ID NO: 952), comprising a first amino acid sequence being at least 90% homologous to MGAMTQLLAGVFLAFLALATEGGVLKKVIRHKRQSGVNATLPEENQPVVFNHVYNIK LPVGSQCSVDLESASGEKDLAPPSEPSESFQEHTVDGENQIVFTHRINIPRRACGCAAAP DVKELLSRLEELENLVSSLREQCTAGAGCCLQPATGRLDTRPFCSGRGNFSTEGCGCVC EPGWKGPNCSEPECPGNCHLRGRCIDGQCICDDGFTGEDCSQLACPSDCNDQGKCVNG VCICFEGYAGADCSREICPVPCSEEHGTCVDGLCVCHDGFAGDDCNKPLCLNNCYNRG RCVENECVCDEGFTGEDCSELICPNDCFDRGRCINGTCYCEEGFTGEDCGKPTCPHACH TQGRCEEGQCVCDEGFAGVDCSEKRCPADCHNRGRCVDGRCECDDGFTGADCGELKC PNGCSGHGRCVNGQCVCDEGYTGEDCSQLRCPNDCHSRGRCVEGKCVCEQGFKGYDC SDMSCPNDCHQHGRCVNGMCVCDDGYTGEDCRDRQCPRDCSNRGLCVDGQCVCEDG FTGPDCAELSCPNDCHGRGRCVNGQCVCHEGFMGKDCKEQRCPSDCHGQGRCVDGQ CICHEGFTGLDCGQHSCPSDCNNLGQCVSGRCICNEGYSGEDCSEVSPPKDLVVTEVTE ETVNLAWDNEMRVTEYLVVYTPTHEGGLEMQFRVPGDQTSTIIQELEPGVEYFIRVFAI LENKKSIPVSARVAT corresponding to amino acids 1-710 of TENA_HUMAN_V1 (SEQ ID NO: 1011), which also corresponds to amino acids 1-710 of HUMTEN_PEA_(—)1_P32 (SEQ ID NO: 952), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence CE corresponding to amino acids 711-712 of HUMTEN_PEA_(—)1_P32 (SEQ ID NO: 952), wherein said first amino acid sequence and second amino acid sequence are contiguous and in a sequential order.

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for HUMOSTRO_PEA_(—)1_PEA_(—)1_P21 (SEQ ID NO: 311), comprising a first amino acid sequence being at least 90% homologous to MRIAVICFCLLGITCAIPVKQADSGSSEEKQLYNKYPDAVATWLNPDPSQKQNLLAPQ corresponding to amino acids 1-58 of OSTP_HUMAN, which also corresponds to amino acids 1-58 of HUMOSTRO_PEA_(—)1_PEA_(—)1_P21 (SEQ ID NO: 311), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence VFLNFS (SEQ ID NO: 1108) corresponding to amino acids 59-64 of HUMOSTRO_PEA_(—)1_PEA_(—)1_P21 (SEQ ID NO: 311), wherein said first amino acid sequence and second amino acid sequence are contiguous and in a sequential order.

According to preferred embodiments of the present invention, there is provided an isolated polypeptide encoding for a tail of HUMOSTRO_PEA_(—)1_PEA_(—)1_P21 (SEQ ID NO: 311), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence VFLNFS (SEQ ID NO: 1108) in HUMOSTRO_PEA_(—)1_PEA_(—)1_P21 (SEQ ID NO: 311).

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for HUMOSTRO_PEA_(—)1_PEA_(—)1_P25 (SEQ ID NO: 312), comprising a first amino acid sequence being at least 90% homologous to MRIAVICFCLLGITCAIPVKQADSGSSEEKQ corresponding to amino acids 1-31 of OSTP_HUMAN, which also corresponds to amino acids 1-31 of HUMOSTRO_PEA_(—)1_PEA_(—)1_P25 (SEQ ID NO: 312), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence H corresponding to amino acids 32-32 of HUMOSTRO_PEA_(—)1_PEA_(—)1_P25 (SEQ ID NO: 312), wherein said first amino acid sequence and second amino acid sequence are contiguous and in a sequential order.

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for HUMOSTRO_PEA_(—)1_PEA_(—)1_P30 (SEQ ID NO: 313), comprising a first amino acid sequence being at least 90% homologous to MRIAVICFCLLGITCAIPVKQADSGSSEEKQ corresponding to amino acids 1-31 of OSTP_HUMAN, which also corresponds to amino acids 1-31 of HUMOSTRO_PEA_(—)1_PEA_(—)1_P30 (SEQ ID NO: 313), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence VSIFYVFI (SEQ ID NO: 1109) corresponding to amino acids 32-39 of HUMOSTRO_PEA_(—)1_PEA_(—)1_P30 (SEQ ID NO: 313), wherein said first amino acid sequence and second amino acid sequence are contiguous and in a sequential order.

According to preferred embodiments of the present invention, there is provided an isolated polypeptide encoding for a tail of HUMOSTRO_PEA_(—)1_PEA_(—)1_P30 (SEQ ID NO: 313), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence VSIFYVFI (SEQ ID NO: 1109) in HUMOSTRO_PEA_(—)1_PEA_(—)1_P30 (SEQ ID NO: 313).

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for H61775_P16 (SEQ ID NO: 9), comprising a first amino acid sequence being at least 90% homologous to MVWCLGLAVLSLVISQGADGRGKPEVVSVVGRAGESVVLGCDLLPPAGRPPLHVIEWL RFGFLLPIFIQFGLYSPRIDPDYVG corresponding to amino acids 11-93 of Q9P2J2 (SEQ ID NO: 953), which also corresponds to amino acids 1-83 of H61775_P16 (SEQ ID NO: 9), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence DCGFPAFRELKRAETVSPVFFTRRCIWEDLKSTGFSPAGGGRPPGGGPRTQEDSGLPCW RSSCSVTLQV (SEQ ID NO: 1110) corresponding to amino acids 84-152 of H61775_P16 (SEQ ID NO: 9), wherein said first and second amino acid sequences are contiguous and in a sequential order.

According to preferred embodiments of the present invention, there is provided an isolated polypeptide encoding for a tail of H61775_P16 (SEQ ID NO: 9), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence DCGFPAFRELKRAETVSPVFFTRRCIWEDLKSTGFSPAGGGRPPGGGPRTQEDSGLPCW RSSCSVTLQV (SEQ ID NO: 1110) in H61775_P16 (SEQ ID NO: 9).

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for H61775_P16 (SEQ ID NO: 9), comprising a first amino acid sequence being at least 90% homologous to MVWCLGLAVLSLVISQGADGRGKPEVVSVVGRAGESVVLGCDLLPPAGRPPLHVIEWL RFGFLLPIFIQFGLYSPRIDPDYVG corresponding to amino acids 1-83 of AAQ88495 (SEQ ID NO: 954), which also corresponds to amino acids 1-83 of H61775_P16 (SEQ ID NO: 9), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence DCGFPAFRELKRAETVSPVFFTRRCIWEDLKSTGFSPAGGGRPPGGGPRTQEDSGLPCW RSSCSVTLQV (SEQ ID NO: 1110) corresponding to amino acids 84-152 of H61775_P16 (SEQ ID NO: 9), wherein said first and second amino acid sequences are contiguous and in a sequential order.

According to preferred embodiments of the present invention, there is provided an isolated polypeptide encoding for a tail of H61775_P16 (SEQ ID NO: 9), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence DCGFPAFRELKRAETVSPVFFTRRCIWEDLKSTGFSPAGGGRPPGGGPRTQEDSGLPCW RSSCSVTLQV (SEQ ID NO: 1110) in H61775_P16 (SEQ ID NO: 9).

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for H61775_P17 (SEQ ID NO: 10), comprising a first amino acid sequence being at least 90% homologous to MVWCLGLAVLSLVISQGADGRGKPEVVSVVGRAGESVVLGCDLLPPAGRPPLHVIEWL RFGFLLPIFIQFGLYSPRIDPDYVG corresponding to amino acids 11-93 of Q9P2J2 (SEQ ID NO: 953), which also corresponds to amino acids 1-83 of H61775_P17 (SEQ ID NO: 10).

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for H61775_P17 (SEQ ID NO: 10), comprising a first amino acid sequence being at least 90% homologous to MVWCLGLAVLSLVISQGADGRGKPEVVSVVGRAGESVVLGCDLLPPAGRPPLHVIEWL RFGFLLPIFIQFGLYSPRIDPDYVG corresponding to amino acids 1-83 of AAQ88495 (SEQ ID NO: 954), which also corresponds to amino acids 1-83 of H61775_P17 (SEQ ID NO: 10).

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for HSAPHOL_P2 (SEQ ID NO: 37), comprising a first amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence PHSGPAAAFIRRRGWWPGPRCA (SEQ ID NO: 1111) corresponding to amino acids 1-22 of HSAPHOL_P2 (SEQ ID NO: 37), second amino acid sequence being at least 90% homologous to PATPRPLSWLRAPTRLCLDGPSPVLCA corresponding to amino acids 1-27 of AAH21289, which also corresponds to amino acids 23-49 of HSAPHOL_P2 (SEQ ID NO: 37), and a third amino acid sequence being at least 90% homologous to EKEKDPKYWRDQAQETLKYALELQKLNTNVAKNVIMFLGDGMGVSTVTAARILKGQL HHNPGEETRLEMDKFPFVALSKTYNTNAQVPDSAGTATAYLCGVKANEGTVGVSAAT ERSRCNTTQGNEVTSILRWAKDAGKSVGIVTTTRVNHATPSAAYAHSADRDWYSDNE MPPEALSQGCKDIAYQLMHNIRDIDVIMGGGRKYMYPKNKTDVEYESDEKARGTRLD GLDLVDTWKSFKPRYKHSHFIWNRTELLTLDPHNVDYLLGLFEPGDMQYELNRNNVT DPSLSEMVVVAIQILRKNPKGFFLLVEGGRIDHGHHEGKAKQALHEAVEMDRAIGQAG SLTSSEDTLTVVTADHSHVFTFGGYTPRGNSIFGLAPMLSDTDKKPFTAILYGNGPGYK VVGGERENVSMVDYAHNNYQAQSAVPLRHETHGGEDVAVFSKGPMAHLLHGVHEQN YVPHVMAYAACIGANLGHCAPASSAGSLAAGPLLLALALYPLSVLF corresponding to amino acids 83-586 of AAH21289, which also corresponds to amino acids 50-553 of HSAPHOL_P2 (SEQ ID NO: 37), wherein said first, second and third amino acid sequences are contiguous and in a sequential order.

According to preferred embodiments of the present invention, there is provided an isolated polypeptide encoding for a head of HSAPHOL_P2 (SEQ ID NO: 37), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence PHSGPAAAFIRRRGWWPGPRCA (SEQ ID NO: 1111) of HSAPHOL_P2 (SEQ ID NO: 37).

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for an edge portion of HSAPHOL_P2 (SEQ ID NO: 37), comprising a polypeptide having a length “n”, wherein n is at least about 10 amino acids in length, optionally at least about 20 amino acids in length, preferably at least about 30 amino acids in length, more preferably at least about 40 amino acids in length and most preferably at least about 50 amino acids in length, wherein at least two amino acids comprise AE, having a structure as follows: a sequence starting from any of amino acid numbers 49-x to 50; and ending at any of amino acid numbers 50+((n−2)−x), in which x varies from 0 to n−2.

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for HSAPHOL_P2 (SEQ ID NO: 37), comprising a first amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence PHSGPAAAFIRRRGWWPGPRCAPATPRPLSWLRAPTRLCLDGPSPVLCA corresponding to amino acids 1-49 of HSAPHOL_P2 (SEQ ID NO: 37), second amino acid sequence being at least 90% homologous to EKEKDPKYWRDQAQETLKYALELQKLNTNVAKNVIMFLGDGMGVSTVTAARILKGQL HHNPGEETRLEMDKFPFVALSKTYNTNAQVPDSAGTATAYLCGVKANEGTVGVSAAT ERSRCNTTQGNEVTSILRWAKDAGKSVGIVTTTRVNHATPSAAYAHSADRDWYSDNE MPPEALSQGCKDIAYQLMHNIRDIDVIMGGGRKYMYPKNKTDVEYESDEKARGTRLD GLDLVDTWKSFKPRYKHSHFIWNRTELLTLDPHNVDYLLGLFEPGDMQYELNRNNVT DPSLSEMVVVAIQILRKNPKGFFLLVEGGRIDHGHHEGKAKQALHEAVEMDRAIGQAG SLTSSEDTLTVVTADHSHVFTFGGYTPRGNSIFGLAPMLSDTDKKPFTAILYGNGPGYK VVGGERENVSMVDYAHNNYQAQSAVPLRHETHGGEDVAVFSKGPMAHLLHGVHEQN YVPHVMAYAACIGANLGHCAPASSAGSLAAGPLLLALALYPLSVLF corresponding to amino acids 21-524 of PPBT_HUMAN, which also corresponds to amino acids 50-553 of HSAPHOL_P2 (SEQ ID NO: 37), wherein said first, second and third amino acid sequences are contiguous and in a sequential order.

According to preferred embodiments of the present invention, there is provided an isolated polypeptide encoding for a head of HSAPHOL_P2 (SEQ ID NO: 37), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence PHSGPAAAFIRRRGWWPGPRCAPATPRPLSWLRAPTRLCLDGPSPVLCA of HSAPHOL_P2 (SEQ ID NO: 37).

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for an edge portion of HSAPHOL_P2 (SEQ ID NO: 37), comprising a polypeptide having a length “n”, wherein n is at least about 10 amino acids in length, optionally at least about 20 amino acids in length, preferably at least about 30 amino acids in length, more preferably at least about 40 amino acids in length and most preferably at least about 50 amino acids in length, wherein at least two amino acids comprise AE, having a structure as follows: a sequence starting from any of amino acid numbers 49-x to 50; and ending at any of amino acid numbers 50+((n−2)−x), in which x varies from 0 to n−2.

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for HSAPHOL_P3 (SEQ ID NO: 38), comprising a first amino acid sequence being at least 90% homologous to MISPFLVLAIGTCLTNSLVP corresponding to amino acids 63-82 of AAH21289, which also corresponds to amino acids 1-20 of HSAPHOL_P3 (SEQ ID NO: 38), and a second amino acid sequence being at least 90% homologous to GMGVSTVTAARILKGQLHHNPGEETRLEMDKFPFVALSKTYNTNAQVPDSAGTATAYL CGVKANEGTVGVSAATERSRCNTTQGNEVTSILRWAKDAGKSVGIVTTTRVNHATPSA AYAHSADRDWYSDNEMPPEALSQGCKDIAYQLMHNIRDIDVIMGGGRKYMYPKNKTD VEYESDEKARGTRLDGLDLVDTWKSFKPRYKHSHFIWNRTELLTLDPHNVDYLLGLFE PGDMQYELNRNNVTDPSLSEMVVVAIQILRKNPKGFFLLVEGGRIDHGHHEGKAKQAL HEAVEMDRAIGQAGSLTSSEDTLTVVTADHSHVFTFGGYTPRGNSIFGLAPMLSDTDKK PFTAILYGNGPGYKVVGGERENVSMVDYAHNNYQAQSAVPLRHETHGGEDVAVFSKG PMAHLLHGVHEQNYVPHVMAYAACIGANLGHCAPASSAGSLAAGPLLLALALYPLSV LF corresponding to amino acids 123-586 of AAH21289, which also corresponds to amino acids 21-484 of HSAPHOL_P3 (SEQ ID NO: 38), wherein said first and second amino acid sequences are contiguous and in a sequential order.

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for an edge portion of HSAPHOL_P3 (SEQ ID NO: 38), comprising a polypeptide having a length “n”, wherein n is at least about 10 amino acids in length, optionally at least about 20 amino acids in length, preferably at least about 30 amino acids in length, more preferably at least about 40 amino acids in length and most preferably at least about 50 amino acids in length, wherein at least two amino acids comprise PG, having a structure as follows: a sequence starting from any of amino acid numbers 20-x to 20; and ending at any of amino acid numbers 21+((n−2)−x), in which x varies from 0 to n−2.

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for HSAPHOL_P3 (SEQ ID NO: 38), comprising a first amino acid sequence being at least 90% homologous to MISPFLVLAIGTCLTNSLVP corresponding to amino acids 1-20 of PPBT_HUMAN, which also corresponds to amino acids 1-20 of HSAPHOL_P3 (SEQ-ID NO: 38), and a second amino acid sequence being at least 90% homologous to GMGVSTVTAARILKGQLHHNPGEETRLEMDKFPFVALSKTYNTNAQVPDSAGTATAYL CGVKANEGTVGVSAATERSRCNTTQGNEVTSILRWAKDAGKSVGIVTTTRVNHATPSA AYAHSADRDWYSDNEMPPEALSQGCKDIAYQLMHNIRDIDVIMGGGRKYMYPKNKTD VEYESDEKARGTRLDGLDLVDTWKSFKPRYKHSHFIWNRTELLTLDPHNVDYLLGLFE PGDMQYELNRNNVTDPSLSEMVVVAIQILRKNPKGFFLLVEGGRIDHGHHEGKAKQAL HEAVEMDRAIGQAGSLTSSEDTLTVVTADHSHVFTFGGYTPRGNSIFGLAPMLSDTDKK PFTAILYGNGPGYKVVGGERENVSMVDYAHNNYQAQSAVPLRHETHGGEDVAVFSKG PMAHLLHGVHEQNYVPHVMAYAACIGANLGHCAPASSAGSLAAGPLLLALALYPLSV LF corresponding to amino acids 61-524 of PPBT_HUMAN, which also corresponds to amino acids 21-484 of HSAPHOL_P3 (SEQ ID NO: 38), wherein said first and second amino acid sequences are contiguous and in a sequential order.

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for an edge portion of HSAPHOL_P3 (SEQ ID NO: 38), comprising a polypeptide having a length “n”, wherein n is at least about 10 amino acids in length, optionally at least about 20 amino acids in length, preferably at least about 30 amino acids in length, more preferably at least about 40 amino acids in length and most preferably at least about 50 amino acids in length, wherein at least two amino acids comprise PG, having a structure as follows: a sequence starting from any of amino acid numbers 20-x to 20; and ending at any of amino acid numbers 21+((n−2)−x), in which x varies from 0 to n−2.

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for HSAPHOL_P4 (SEQ ID NO: 39), comprising a first amino acid sequence being at least 90% homologous to MGVSTVTAARILKGQLHHNPGEETRLEMDKFPFVALSKTYNTNAQVPDSAGTATAYLC GVKANEGTVGVSAATERSRCNTTQGNEVTSILRWAKDAGKSVGIVTTTRVNHATPSAA YAHSADRDWYSDNEMPPEALSQGCKDIAYQLMHNIRDIDVIMGGGRKYMYPKNKTDV EYESDEKARGTRLDGLDLVDTWKSFKPRYKHSHFIWNRTELLTLDPHNVDYLLGLFEP GDMQYELNRNNVTDPSLSEMVVVAIQILRKNPKGFFLLVEGGRIDHGHHEGKAKQALH EAVEMDRAIGQAGSLTSSEDTLTVVTADHSHVFTFGGYTPRGNSIFGLAPMLSDTDKKP FTAILYGNGPGYKVVGGERENVSMVDYAHNNYQAQSAVPLRHETHGGEDVAVFSKGP MAHLLHGVHEQNYVPHVMAYAACIGANLGHCAPASSAGSLAAGPLLLALALYPLSVL F corresponding to amino acids 124-586 of AAH21289, which also corresponds to amino acids 1-463 of HSAPHOL_P4 (SEQ ID NO: 39).

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for HSAPHOL_P4 (SEQ ID NO: 39), comprising a first amino acid sequence being at least 90% homologous to MGVSTVTAARILKGQLHHNPGEETRLEMDKFPFVALSKTYNTNAQVPDSAGTATAYLC GVKANEGTVGVSAATERSRCNTTQGNEVTSILRWAKDAGKSVGIVTTTRVNHATPSAA YAHSADRDWYSDNEMPPEALSQGCKDIAYQLMHNIRDIDVIMGGGRKYMYPKNKTDV EYESDEKARGTRLDGLDLVDTWKSFKPRYKHSHFIWNRTELLTLDPHNVDYLLGLFEP GDMQYELNRNNVTDPSLSEMVVVAIQILRKNPKGFFLLVEGGRIDHGHHEGKAKQALH EAVEMDRAIGQAGSLTSSEDTLTVVTADHSHVFTFGGYTPRGNSIFGLAPMLSDTDKKP FTAILYGNGPGYKVVGGERENVSMVDYAHNNYQAQSAVPLRHETHGGEDVAVFSKGP MAHLLHGVHEQNYVPHVMAYAACIGANLGHCAPASSAGSLAAGPLLLALALYPLSVL F corresponding to amino acids 62-524 of PPBT_HUMAN, which also corresponds to amino acids 1-463 of HSAPHOL_P4 (SEQ ID NO: 39).

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for HSAPHOL_P5 (SEQ ID NO: 40), comprising a first amino acid sequence being at least 90% homologous to MISPFLVLAIGTCLTNSLVPEKEKDPKYWRDQAQETLKYALELQKLNTNVAKNVIMFL GDGMGVSTVTAARILKGQLHHNPGEETRLEMDKFPFVALSKTYNTNAQVPDSAGTAT AYLCGVKANEGTVGVSAATERSRCNTTQGNEVTSILRWAKDAGKSVGIVTTTRVNHA TPSAAYAHSADRDWYSDNEMPPEALSQGCKDIAYQLMHNIRDIDVIMGGGRKYMYPK NKTDVEYESDEKARGTRLDGLDLVDTWKSFKPRYKHSHFIWNRTELLTLDPHNVDYLL GLFEPGDMQYELNRNNVTDPSLSEMVVVAIQILRKNPKGFFLLVEGGRIDHGHHEGKA KQALHEAVEM corresponding to amino acids 63-417 of AAH21289, which also corresponds to amino acids 1-355 of HSAPHOL_P5 (SEQ ID NO: 40), and a second amino acid sequence being at least 90% homologous to DHSHVFTFGGYTPRGNSIFGLAPMLSDTDKKPFTAILYGNGPGYKVVGGERENVSMVD YAHNNYQAQSAVPLRHETHGGEDVAVFSKGPMAHLLHGVHEQNYVPHVMAYAACIG ANLGHCAPASSAGSLAAGPLLLALALYPLSVLF corresponding to amino acids 440-586 of AAH21289, which also corresponds to amino acids 356-502 of HSAPHOL_P5 (SEQ ID NO: 40), wherein said first and second amino acid sequences are contiguous and in a sequential order.

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for an edge portion of HSAPHOL_P5 (SEQ ID NO: 40), comprising a polypeptide having a length “n”, wherein n is at least about 10 amino acids in length, optionally at least about 20 amino acids in length, preferably at least about 30 amino acids in length, more preferably at least about 40 amino acids in length and most preferably at least about 50 amino acids in length, wherein at least two amino acids comprise MD, having a structure as follows: a sequence starting from any of amino acid numbers 355-x to 355; and ending at any of amino acid numbers 356+((n−2)−x), in which x varies from 0 to n−2.

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for HSAPHOL_P5 (SEQ ID NO: 40), comprising a first amino acid sequence being at least 90% homologous to MISPFLVLAIGTCLTNSLVPEKEKDPKYWRDQAQETLKYALELQKLNTNVAKNVIMFL GDGMGVSTVTAARILKGQLHHNPGEETRLEMDKFPFVALSKTYNTNAQVPDSAGTAT AYLCGVKANEGTVGVSAATERSRCNTTQGNEVTSILRWAKDAGKSVGIVTTTRVNHA TPSAAYAHSADRDWYSDNEMPPEALSQGCKDIAYQLMHNIRDIDVIMGGGRKYMYPK NKTDVEYESDEKARGTRLDGLDLVDTWKSFKPRYKHSHFIWNRTELLTLDPHNVDYLL GLFEPGDMQYELNRNNVTDPSLSEMVVVAIQILRKNPKGFFLLVEGGRIDHGHHEGKA KQALHEAVEM corresponding to amino acids 1-355 of PPBT_HUMAN, which also corresponds to amino acids 1-355 of HSAPHOL_P5 (SEQ ID NO: 40), and a second amino acid sequence being at least 90% homologous to DHSHVFTFGGYTPRGNSIFGLAPMLSDTDKKPFTAILYGNGPGYKVVGGERENVSMVD YAHNNYQAQSAVPLRHETHGGEDVAVFSKGPMAHLLHGVHEQNYVPHVMAYAACIG ANLGHCAPASSAGSLAAGPLLLALALYPLSVLF corresponding to amino acids 377-524 of PPBT_HUMAN, which also corresponds to amino acids 356-502 of HSAPHOL_P5 (SEQ ID NO: 40), wherein said first and second amino acid sequences are contiguous and in a sequential order.

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for an edge portion of HSAPHOL_P5 (SEQ ID NO: 40), comprising a polypeptide having a length “n”, wherein n is at least about 10 amino acids in length, optionally at least about 20 amino acids in length, preferably at least about 30 amino acids in length, more preferably at least about 40 amino acids in length and most preferably at least about 50 amino acids in length, wherein at least two amino acids comprise MD, having a structure as follows: a sequence starting from any of amino acid numbers 355-x to 355; and ending at any of amino acid numbers 356+((n−2)−x), in which x varies from 0 to n−2.

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for HSAPHOL_P6 (SEQ ID NO: 41), comprising a first amino acid sequence being at least 90% homologous to MISPFLVLAIGTCLTNSLVPEKEKDPKYWRDQAQETLKYALELQKLNTNVAKNVIMFL GDGMGVSTVTAARILKGQLHHNPGEETRLEMDKFPFVALSKTYNTNAQVPDSAGTAT AYLCGVKANEGTVGVSAATERSRCNTTQGNEVTSILRWAKDAGKSVGIVTTTRVNHA TPSAAYAHSADRDWYSDNEMPPEALSQGCKDIAYQLMHNIRDIDVIMGGGRKYMYPK NKTDVEYESDEKARGTRLDGLDLVDTWKSFKPRYKHSHFIWNRTELLTLDPHNVDYLL corresponding to amino acids 63-349 of AAH21289, which also corresponds to amino acids 1-287 of HSAPHOL_P6 (SEQ ID NO: 41), and a second amino acid sequence being at least 90% homologous to GGRIDHGHHEGKAKQALHEAVEMDRAIGQAGSLTSSEDTLTVVTADHSHVFTFGGYTP RGNSIFGLAPMLSDTDKKPFTAILYGNGPGYKVVGGERENVSMVDYAHNNYQAQSAV PLRHETHGGEDVAVFSKGPMAHLLHGVHEQNYVPHVMAYAACIGANLGHCAPASSAG SLAAGPLLLALALYPLSVLF corresponding to amino acids 395-586 of AAH21289, which also corresponds to amino acids 288-479 of HSAPHOL_P6 (SEQ ID NO: 41), wherein said first and second amino acid sequences are contiguous and in a sequential order.

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for an edge portion of HSAPHOL_P6 (SEQ ID NO: 41), comprising a polypeptide having a length “n”, wherein n is at least about 10 amino acids in length, optionally at least about 20 amino acids in length, preferably at least about 30 amino acids in length, more preferably at least about 40 amino acids in length and most preferably at least about 50 amino acids in length, wherein at least two amino acids comprise LG, having a

structure as follows: a sequence starting from any of amino acid numbers 287-x to 287; and ending at any of amino acid numbers 288+((n−2)−x), in which x varies from 0 to n−2.

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for HSAPHOL_P6 (SEQ ID NO: 41), comprising a first amino acid sequence being at least 90% homologous to MISPFLVLAIGTCLTNSLVPEKEKDPKYWRDQAQETLKYALELQKLNTNVAKNVIMFL GDGMGVSTVTAARILKGQLHHNPGEETRLEMDKFPFVALSKTYNTNAQVPDSAGTAT AYLCGVKANEGTVGVSAATERSRCNTTQGNEVTSILRWAKDAGKSVGIVTTTRVNHA TPSAAYAHSADRDWYSDNEMPPEALSQGCKDIAYQLMHNIRDIDVIMGGGRKYMYPK NKTDVEYESDEKARGTRLDGLDLVDTWKSFKPRYKHSHFIWNRTELLTLDPHNVDYLL corresponding to amino acids 1-287 of PPBT_HUMAN, which also corresponds to amino acids 1-287 of HSAPHOL_P6 (SEQ ID NO: 41), and a second amino acid sequence being at least 90% homologous to GGRIDHGHHEGKAKQALHEAVEMDRAIGQAGSLTSSEDTLTVVTADHSHVFTFGGYTP RGNSIFGLAPMLSDTDKKPFTAILYGNGPGYKVVGGERENVSMVDYAHNNYQAQSAV PLRHETHGGEDVAVFSKGPMAHLLHGVHEQNYVPHVMAYAACIGANLGHCAPASSAG SLAAGPLLLALALYPLSVLF corresponding to amino acids 333-524 of PPBT_HUMAN, which also corresponds to amino acids 288-479 of HSAPHOL_P6 (SEQ ID NO: 41), wherein said first and second amino acid sequences are contiguous and in a sequential order.

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for an edge portion of HSAPHOL_P6 (SEQ ID NO: 41), comprising a polypeptide having a length “n”, wherein n is at least about 10 amino acids in length, optionally at least about 20 amino acids in length, preferably at least about 30 amino acids in length, more preferably at least about 40 amino acids in length and most preferably at least about 50 amino acids in length, wherein at least two amino acids comprise LG, having a structure as follows: a sequence starting from any of amino acid numbers 287-x to 287; and ending at any of amino acid numbers 288+((n−2)−x), in which x varies from 0 to n−2.

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for HSAPHOL_P7 (SEQ ID NO: 42), comprising a first amino acid sequence being at least 90% homologous to MISPFLVLAIGTCLTNSLVPEKEKDPKYWRDQAQETLKYALELQKLNTNVAKNVIMFL GDGMGVSTVTAARILKGQLHHNPGEETRLEMDKFPFVALSKTYNTNAQVPDSAGTAT AYLCGVKANEGTVGVSAATERSRCNTTQGNEVTSILRWAKDAGKSVGIVTTTRVNHA TPSAAYAHSADRDWYSDNEMPPEALSQGCKDIAYQLMHNIRDIDVIMGGGRKYMYPK NKTDVEYESDEKARGTRLDGLDLVDTWKSFKPRYK corresponding to amino acids 63-326 of AAH21289, which also corresponds to amino acids 1-264 of HSAPHOL_P7 (SEQ ID NO: 42), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence LPPRCPLANRVDFSWAGREYRLQTFSKPLIFLANVFLQTQRP (SEQ ID NO: 1112) corresponding to amino acids 265-306 of HSAPHOL_P7 (SEQ ID NO: 42), wherein said first and second amino acid sequences are contiguous and in a sequential order.

According to preferred embodiments of the present invention, there is provided an isolated polypeptide encoding for a tail of HSAPHOL_P7 (SEQ ID NO: 42), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence LPPRCPLANRVDFSWAGREYRLQTFSKPLIFLANVFLQTQRP (SEQ ID NO: 1112) in HSAPHOL_P7 (SEQ ID NO: 42).

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for HSAPHOL_P7 (SEQ ID NO: 42), comprising a first amino acid sequence being at least 90% homologous to MISPFLVLAIGTCLTNSLVPEKEKDPKYWRDQAQETLKYALELQKLNTNVAKNVIMFL GDGMGVSTVTAARILKGQLHHNPGEETRLEMDKFPFVALSKTYNTNAQVPDSAGTAT AYLCGVKANEGTVGVSAATERSRCNTTQGNEVTSILRWAKDAGKSVGIVTTTRVNHA TPSAAYAHSADRDWYSDNEMPPEALSQGCKDIAYQLMHNIRDIDVIMGGGRKYMYPK NKTDVEYESDEKARGTRLDGLDLVDTWKSFKPR corresponding to amino acids 1-262 of PPBT_HUMAN, which also corresponds to amino acids 1-262 of HSAPHOL_P7 (SEQ ID NO: 42), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence YKLPPRCPLANRVDFSWAGREYRLQTFSKPLIFLANVFLQTQRP corresponding to amino acids 263-306 of HSAPHOL_P7 (SEQ ID NO: 42), wherein said first and second amino acid sequences are contiguous and in a sequential order.

According to preferred embodiments of the present invention, there is provided an isolated polypeptide encoding for a tail of HSAPHOL_P7 (SEQ ID NO: 42), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence YKLPPRCPLANRVDFSWAGREYRLQTFSKPLIFLANVFLQTQRP in HSAPHOL_P7 (SEQ ID NO: 42).

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for HSAPHOL_P7 (SEQ ID NO: 42), comprising a first amino acid sequence being at least 90% homologous to MISPFLVLAIGTCLTNSLVPEKEKDPKYWRDQAQETLKYALELQKLNTNVAKNVIMFL GDGMGVSTVTAARILKGQLHHNPGEETRLEMDKFPFVALSKTYNTNAQVPDSAGTAT AYLCGVKANEGTVGVSAATERSRCNTTQGNEVTSILRWAKDAGKSVGIVTTTRVNHA TPSAAYAHSADRDWYSDNEMPPEALSQGCKDIAYQLMHNIRDIDVIMGGGRKYMYPK NKTDVEYESDEKARGTRLDGLDLVDTWKSFKPRYK corresponding to amino acids 1-264 of O75090 (SEQ ID NO: 958), which also corresponds to amino acids 1-264 of HSAPHOL_P7 (SEQ ID NO: 42), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence LPPRCPLANRVDFSWAGREYRLQTFSKPLIFLANVFLQTQRP (SEQ ID NO: 1112) corresponding to amino acids 265-306 of HSAPHOL_P7 (SEQ ID NO: 42), wherein said first and second amino acid sequences are contiguous and in a sequential order.

According to preferred embodiments of the present invention, there is provided an isolated polypeptide encoding for a tail of HSAPHOL_P7 (SEQ ID NO: 42), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence LPPRCPLANRVDFSWAGREYRLQTFSKPLIFLANVFLQTQRP (SEQ ID NO: 1112) in HSAPHOL_P7 (SEQ ID NO: 42).

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for HSAPHOL_P8 (SEQ ID NO: 43), comprising a first amino acid sequence being at least 90% homologous to MISPFLVLAIGTCLTNSLVPEKEKDPKYWRDQAQETLKYALELQKLNTNVAKNVIMFL GDGMGVSTVTAARILKGQLHHNPGEETRLEMDKFPFVALSKTYNTNAQVPDSAGTAT AYLCGVKANEGTVGVSAATERSRCNTTQGNEVTSILRWAKDAGKSVGIVTTTRVNHA TPSAAYAHSADRDWYSDNEMPPEALSQGCKDIAYQLMHNIRDIDVIMGGGRKYMYPK NKTDVEYESDEKARGTRLDGLDLVDTWKSFKPRYKHSHFIWNRTELLTLDPHNVDYLL G corresponding to amino acids 63-350 of AAH21289, which also corresponds to amino acids 1-288 of HSAPHOL_P8 (SEQ ID NO: 43), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence KWRGWRGGCMARSLVAGAACGQHLGTRP (SEQ ID NO: 1113) corresponding to amino acids 289-316 of HSAPHOL_P8 (SEQ ID NO: 43), wherein said first and second amino acid sequences are contiguous and in a sequential order.

According to preferred embodiments of the present invention, there is provided an isolated polypeptide encoding for a tail of HSAPHOL_P8 (SEQ ID NO: 43), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence KWRGWRGGCMARSLVAGAACGQHLGTRP (SEQ ID NO: 1113) in HSAPHOL_P8 (SEQ ID NO: 43).

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for HSAPHOL_P8 (SEQ ID NO: 43), comprising a first amino acid sequence being at least 90% homologous to MISPFLVLAIGTCLTNSLVPEKEKDPKYWRDQAQETLKYALELQKLNTNVAKNVIMFL GDGMGVSTVTAARILKGQLHHNPGEETRLEMDKFPFVALSKTYNTNAQVPDSAGTAT AYLCGVKANEGTVGVSAATERSRCNTTQGNEVTSILRWAKDAGKSVGIVTTTRVNHA TPSAAYAHSADRDWYSDNEMPPEALSQGCKDIAYQLMHNIRDIDVIMGGGRKYMYPK NKTDVEYESDEKARGTRLDGLDLVDTWKSFKPRYKHSHFIWNRTELLTLDPHNVDYLL G corresponding to amino acids 1-288 of PPBT_HUMAN, which also corresponds to amino acids 1-288 of HSAPHOL_P8 (SEQ ID NO: 43), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence KWRGWRGGCMARSLVAGAACGQHLGTRP (SEQ ID NO: 1113) corresponding to amino acids 289-316 of HSAPHOL_P8 (SEQ ID NO: 43), wherein said first and second amino acid sequences are contiguous and in a sequential order.

According to preferred embodiments of the present invention, there is provided an isolated polypeptide encoding for a tail of HSAPHOL_P8 (SEQ ID NO: 43), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence KWRGWRGGCMARSLVAGAACGQHLGTRP (SEQ ID NO: 1113) in HSAPHOL_P8 (SEQ ID NO: 43).

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for HSAPHOL_P8 (SEQ ID NO: 43), comprising a first amino acid sequence being at least 90% homologous to MISPFLVLAIGTCLTNSLVPEKEKDPKYWRDQAQETLKYALELQKLNTNVAKNVIMFL GDGMGVSTVTAARILKGQLHHNPGEETRLEMDKFPFVALSKTYNTNAQVPDSAGTAT AYLCGVKANEGTVGVSAATERSRCNTTQGNEVTSILRWAKDAGKSVGIVTTTRVNHA TPSAAYAHSADRDWYSDNEMPPEALSQGCKDIAYQLMHNIRDIDVIMGGGRKYMYPK NKTDVEYESDEKARGTRLDGLDLVDTWKSFKPRYKHSHFIWNRTELLTLDPHNVDYLL G corresponding to amino acids 1-288 of O75090 (SEQ ID NO: 958), which also corresponds to amino acids 1-288 of HSAPHOL_P8 (SEQ ID NO: 43), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence KWRGWRGGCMARSLVAGAACGQHLGTRP (SEQ ID NO: 1113) corresponding to amino acids 289-316 of HSAPHOL_P8 (SEQ ID NO: 43), wherein said first and second amino acid sequences are contiguous and in a sequential order.

According to preferred embodiments of the present invention, there is provided an isolated polypeptide encoding for a tail of HSAPHOL_P8 (SEQ ID NO: 43), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence KWRGWRGGCMARSLVAGAACGQHLGTRP (SEQ ID NO: 1113) in HSAPHOL_P8 (SEQ ID NO: 43).

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for T10888_PEA_(—)1_P2 (SEQ ID NO: 57), comprising a first amino acid sequence being at least 90% homologous to MGPPSAPPCRLHVPWKEVLLTASLLTFWNPPTTAKLTIESTPFNVAEGKEVLLLAHNLP QNRIGYSWYKGERVDGNSLIVGYVIGTQQATPGPAYSGRETIYPNASLLIQNVTQNDTG FYTLQVIKSDLVNEEATGQFHVYPELPKPSISSNNSNPVEDKDAVAFTCEPEVQNTTYL WWVNGQSLPVSPRLQLSNGNMTLTLLSVKRNDAGSYECEIQNPASANRSDPVTLNVLY GPDVPTISPSKANYRPGENLNLSCHAASNPPAQYSWFINGTFQQSTQELFIPNITVNNSGS YMCQAHNSATGLNRTTVTMITVS corresponding to amino acids 1-319 of CEA6_HUMAN, which also corresponds to amino acids 1-319 of T10888_PEA_(—)1_P2 (SEQ ID NO: 57), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence DWTRP (SEQ ID NO: 1114) corresponding to amino acids 320-324 of T10888_PEA_(—)1_P2 (SEQ ID NO: 57), wherein said first and second amino acid sequences are contiguous and in a sequential order.

According to preferred embodiments of the present invention, there is provided an isolated polypeptide encoding for a tail of T10888_PEA_(—)1_P2 (SEQ ID NO: 57), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence DWTRP (SEQ ID NO: 1114) in T10888_PEA_(—)1_P2 (SEQ ID NO: 57).

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for T10888_PEA_(—)1_P4 (SEQ ID NO: 58), comprising a first amino acid sequence being at least 90% homologous to MGPPSAPPCRLHVPWKEVLLTASLLTFWNPPTTAKLTIESTPFNVAEGKEVLLLAHNLP QNRIGYSWYKGERVDGNSLIVGYVIGTQQATPGPAYSGRETIYPNASLLIQNVTQNDTG FYTLQVIKSDLVNEEATGQFHVYPELPKPSISSNNSNPVEDKDAVAFTCEPEVQNTTYL WWVNGQSLPVSPRLQLSNGNMTLTLLSVKRNDAGSYECEIQNPASANRSDPVTLNVL corresponding to amino acids 1-234 of CEA6_HUMAN, which also corresponds to amino acids 1-234 of T10888_PEA_(—)1_P4 (SEQ ID NO: 58), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence LLLSSQLWPPSASRLECWPGWL (SEQ ID NO: 1115) corresponding to amino acids 235-256 of T10888_PEA_(—)1_P4 (SEQ ID NO: 58), wherein said first and second amino acid sequences are contiguous and in a sequential order.

According to preferred embodiments of the present invention, there is provided an isolated polypeptide encoding for a tail of T10888_PEA_(—)1_P4 (SEQ ID NO: 58), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence LLLSSQLWPPSASRLECWPGWL (SEQ ID NO: 1115) in T10888_PEA_(—)1_P4 (SEQ ID NO: 58).

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for T10888_PEA_(—)1_P4 (SEQ ID NO: 58), comprising a first amino acid sequence being at least 90% homologous to MGPPSAPPCRLHVPWKEVLLTASLLTFWNPPTTAKLTIESTPFNVAEGKEVLLLAHNLP QNRIGYSWYKGERVDGNSLIVGYVIGTQQATPGPAYSGRETIYPNASLLIQNVTQNDTG FYTLQVIKSDLVNEEATGQFHVYPELPKPSISSNNSNPVEDKDAVAFTCEPEVQNTTYL WWVNGQSLPVSPRLQLSNGNMTLTLLSVKRNDAGSYECEIQNPASANRSDPVTLNVL corresponding to amino acids 1-234 of Q13774 (SEQ ID NO: 959), which also corresponds to amino acids 1-234 of T10888_PEA_(—)1_P4 (SEQ ID NO: 58), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence LLLSSQLWPPSASRLECWPGWL (SEQ ID NO: 1115) corresponding to amino acids 235-256 of T10888_PEA_(—)1_P4 (SEQ ID NO: 58), wherein said first and second amino acid sequences are contiguous and in a sequential order.

According to preferred embodiments of the present invention, there is provided an isolated polypeptide encoding for a tail of T10888_PEA_(—)1_P4 (SEQ ID NO: 58), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence LLLSSQLWPPSASRLECWPGWL (SEQ ID NO: 1115) in T10888_PEA_(—)1_P4 (SEQ ID NO: 58).

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for T10888_PEA_(—)1_P5 (SEQ ID NO: 59), comprising a first amino acid sequence being at least 90% homologous to MGPPSAPPCRLHVPWKEVLLTASLLTFWNPPTTAKLTIESTPFNVAEGKEVLLLAHNLP QNRIGYSWYKGERVDGNSLIVGYVIGTQQATPGPAYSGRETIYPNASLLIQNVTQNDTG FYTLQVIKSDLVNEEATGQFHVYPELPKPSISSNNSNPVEDKDAVAFTCEPEVQNTTYL WWVNGQSLPVSPRLQLSNGNMTLTLLSVKRNDAGSYECEIQNPASANRSDPVTLNVLY GPDVPTISPSKANYRPGENLNLSCHAASNPPAQYSWFINGTFQQSTQELFIPNITVNNSGS YMCQAHNSATGLNRTTVTMITVSG corresponding to amino acids 1-320 of CEA6_HUMAN, which also corresponds to amino acids 1-320 of T10888_PEA_(—)1_P5 (SEQ ID NO: 59), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence KWIHEALASHFQVESGSQRRARKKFSFPTCVQGAHANPKFSPEPSQFTSADSFPLVFLFF VVFCFLISHV (SEQ ID NO: 1116) corresponding to amino acids 321-390 of T10888_PEA_(—)1_P5 (SEQ ID NO: 59), wherein said first and second amino acid sequences are contiguous and in a sequential order.

According to preferred embodiments of the present invention, there is provided an isolated polypeptide encoding for a tail of T10888_PEA_(—)1_P5 (SEQ ID NO: 59), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence KWIHEALASHFQVESGSQRRARKKFSFPTCVQGAHANPKFSPEPSQFTSADSFPLVFLFF VVFCFLISHV (SEQ ID NO: 1116) in T10888_PEA_(—)1_P5 (SEQ ID NO: 59).

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for T10888_PEA_(—)1_P6 (SEQ ID NO: 60), comprising a first amino acid sequence being at least 90% homologous to MGPPSAPPCRLHVPWKEVLLTASLLTFWNPPTTAKLTIESTPFNVAEGKEVLLLAHNLP QNRIGYSWYKGERVDGNSLIVGYVIGTQQATPGPAYSGRETIYPNASLLIQNVTQNDTG FYTLQVIKSDLVNEEATGQFHVY corresponding to amino acids 1-141 of CEA6_HUMAN, which also corresponds to amino acids 1-141 of T10888_PEA_(—)1_P6 (SEQ ID NO: 60), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence REYFHMTSGCWGSVLLPTYGIVRPGLCLWPSLHYILYQGLDI (SEQ ID NO: 1117) corresponding to amino acids 142-183 of T10888_PEA_(—)1_P6 (SEQ ID NO: 60), wherein said first and second amino acid sequences are contiguous and in a sequential order.

According to preferred embodiments of the present invention, there is provided an isolated polypeptide encoding for a tail of T10888_PEA_(—)1_P6 (SEQ ID NO: 60), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence REYFHMTSGCWGSVLLPTYGIVRPGLCLWPSLHYILYQGLDI (SEQ ID NO: 1117) in T10888_PEA_(—)1_P6 (SEQ ID NO: 60).

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for HSECADH_P9 (SEQ ID NO: 96), comprising a first amino acid sequence being at least 90% homologous to MGPWSRSLSALLLLLQVSSWLCQEPEPCHPGFDAESYTFTVPRRHLERGRVLGRVNFED CTGRQRTAYFSLDTRFKVGTDGVITVKRPLRFHNPQIHFLVYAWDSTYRKFSTKVTLNT VGHHHRPPPHQASVSGIQAELLTFPNSSPGLRRQKRDWVIPPISCPENEKGPFPKNLVQI KSNKDKEGKVFYSITGQGADTPPVGVFIIERETGWLKVTEPLDRERIATYTLFSHAVSSN GNAVEDPMEILITVTDQNDNKPEFTQEVFKGSVMEG corresponding to amino acids 1-274 of Q9UII7 (SEQ ID NO: 963), which also corresponds to amino acids 1-274 of HSECADH_P9 (SEQ ID NO: 96), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence TACRSRIANSCHSGDSWRNSCFANSDSAALAVSSEESGGQRALTAPRG (SEQ ID NO: 1118) corresponding to amino acids 275-322 of HSECADH_P9 (SEQ ID NO: 96), wherein said first and second amino acid sequences are contiguous and in a sequential order.

According to preferred embodiments of the present invention, there is provided an isolated polypeptide encoding for a tail of HSECADH_P9 (SEQ ID NO: 96), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence TACRSRIANSCHSGDSWRNSCFANSDSAALAVSSEESGGQRALTAPRG (SEQ ID NO: 1118) in HSECADH_P9 (SEQ ID NO: 96).

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for HSECADH_P9 (SEQ ID NO: 96), comprising a first amino acid sequence being at least 90% homologous to MGPWSRSLSALLLLLQVSSWLCQEPEPCHPGFDAESYTFTVPRRHLERGRVLGRVNFED CTGRQRTAYFSLDTRFKVGTDGVITVKRPLRFHNPQIHFLVYAWDSTYRKFSTKVTLNT VGHHHRPPPHQASVSGIQAELLTFPNSSPGLRRQKRDWVIPPISCPENEKGPFPKNLVQI KSNKDKEGKVFYSITGQGADTPPVGVFIIERETGWLKVTEPLDRERIATYTLFSHAVSSN GNAVEDPMEILITVTDQNDNKPEFTQEVFKGSVMEG corresponding to amino acids 1-274 of Q9UII8 (SEQ ID NO: 964), which also corresponds to amino acids 1-274 of HSECADH_P9 (SEQ ID NO: 96), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence TACRSRIANSCHSGDSWRNSCFANSDSAALAVSSEESGGQRALTAPRG (SEQ ID NO: 1118) corresponding to amino acids 275-322 of HSECADH_P9 (SEQ ID NO: 96), wherein said first and second amino acid sequences are contiguous and in a sequential order.

According to preferred embodiments of the present invention, there is provided an isolated polypeptide encoding for a tail of HSECADH_P9 (SEQ ID NO: 96), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence TACRSRIANSCHSGDSWRNSCFANSDSAALAVSSEESGGQRALTAPRG (SEQ ID NO: 1118) in HSECADH_P9 (SEQ ID NO: 96).

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for HSECADH_P9 (SEQ ID NO: 96), comprising a first amino acid sequence being at least 90% homologous to MGPWSRSLSALLLLLQVSSWLCQEPEPCHPGFDAESYTFTVPRRHLERGRVLGRVNFED CTGRQRTAYFSLDTRFKVGTDGVITVKRPLRFHNPQIHFLVYAWDSTYRKFSTKVTLNT VGHHHRPPPHQASVSGIQAELLTFPNSSPGLRRQKRDWVIPPISCPENEKGPFPKNLVQI KSNKDKEGKVFYSITGQGADTPPVGVFIIERETGWLKVTEPLDRERIATYTLFSHAVSSN GNAVEDPMEILITVTDQNDNKPEFTQEVFKGSVMEG corresponding to amino acids 1-274 of CAD1_HUMAN, which also corresponds to amino acids 1-274 of HSECADH_P9 (SEQ ID NO: 96), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence TACRSRIANSCHSGDSWRNSCFANSDSAALAVSSEESGGQRALTAPRG (SEQ ID NO: 1118) corresponding to amino acids 275-322 of HSECADH_P9 (SEQ ID NO: 96), wherein said first and second amino acid sequences are contiguous and in a sequential order.

According to preferred embodiments of the present invention, there is provided an isolated polypeptide encoding for a tail of HSECADH_P9 (SEQ ID NO: 96), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence TACRSRIANSCHSGDSWRNSCFANSDSAALAVSSEESGGQRALTAPRG (SEQ ID NO: 1118) in HSECADH_P9 (SEQ ID NO: 96).

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for HSECADH_P13 (SEQ ID NO: 97), comprising a first amino acid sequence being at least 90% homologous to MGPWSRSLSALLLLLQVSSWLCQEPEPCHPGFDAESYTFTVPRRHLERGRVLGRVNFED CTGRQRTAYFSLDTRFKVGTDGVITVKRPLRFHNPQIHFLVYAWDSTYRKFSTKVTLNT VGHHHRPPPHQASVSGIQAELLTFPNSSPGLRRQKRDWVIPPISCPENEKGPFPKNLVQI KSNKDKEGKVFYSITGQGADTPPVGVFIIERETGWLKVTEPLDRERIATYTLFSHAVSSN GNAVEDPMEILITVTDQNDNKPEFTQEVFKGSVMEGALPGTSVMEVTATDADDDVNT YNAAIAYTILSQDPELPDKNMFTINRNTGVISVVTTGLDRESFPTYTLVVQAADLQGEGL STTATAVITVTDTNDNPPIFNPTT corresponding to amino acids 1-379 of Q9UII7 (SEQ ID NO: 963), which also corresponds to amino acids 1-379 of HSECADH_P13 (SEQ ID NO: 97), and a second amino acid sequence VIL corresponding to amino acids 380-382 of HSECADH_P13 (SEQ ID NO: 97), wherein said first and second amino acid sequences are contiguous and in a sequential order.

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for HSECADH_P13 (SEQ ID NO: 97), comprising a first amino acid sequence being at least 90% homologous to MGPWSRSLSALLLLLQVSSWLCQEPEPCHPGFDAESYTFTVPRRHLERGRVLGRVNFED CTGRQRTAYFSLDTRFKVGTDGVITVKRPLRFHNPQIHFLVYAWDSTYRKFSTKVTLNT VGHHHRPPPHQASVSGIQAELLTFPNSSPGLRRQKRDWVIPPISCPENEKGPFPKNLVQI KSNKDKEGKVFYSITGQGADTPPVGVFIIERETGWLKVTEPLDRERIATYTLFSHAVSSN GNAVEDPMEILITVTDQNDNKPEFTQEVFKGSVMEGALPGTSVMEVTATDADDDVNT YNAAIAYTILSQDPELPDKNMFTINRNTGVISVVTTGLDRESFPTYTLVVQAADLQGEGL STTATAVITVTDTNDNPPIFNPTT corresponding to amino acids 1-379 of Q9UII8 (SEQ ID NO: 964), which also corresponds to amino acids 1-379 of HSECADH_P13 (SEQ ID NO: 97), and a second amino acid sequence VIL corresponding to amino acids 380-382 of HSECADH_P13 (SEQ ID NO: 97), wherein said first and second amino acid sequences are contiguous and in a sequential order.

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for HSECADH_P13 (SEQ ID NO: 97), comprising a first amino acid sequence being at least 90% homologous to MGPWSRSLSALLLLLQVSSWLCQEPEPCHPGFDAESYTFTVPRRHLERGRVLGRVNFED CTGRQRTAYFSLDTRFKVGTDGVITVKRPLRFHNPQIHFLVYAWDSTYRKFSTKVTLNT VGHHHRPPPHQASVSGIQAELLTFPNSSPGLRRQKRDWVIPPISCPENEKGPFPKNLVQI KSNKDKEGKVFYSITGQGADTPPVGVFIIERETGWLKVTEPLDRERIATYTLFSHAVSSN GNAVEDPMEILITVTDQNDNKPEFTQEVFKGSVMEGALPGTSVMEVTATDADDDVNT YNAAIAYTILSQDPELPDKNMFTINRNTGVISVVTTGLDRESFPTYTLVVQAADLQGEGL STTATAVITVTDTNDNPPIFNPTT corresponding to amino acids 1-379 of CAD1_HUMAN, which also corresponds to amino acids 1-379 of HSECADH_P13 (SEQ ID NO: 97), and a second amino acid sequence VIL corresponding to amino acids 380-382 of HSECADH_P13 (SEQ ID NO: 971, wherein said first and second amino acid sequences are contiguous and in a sequential order.

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for HSECADH_P14 (SEQ ID NO: 98), comprising a first amino acid sequence being at least 90% homologous to MGPWSRSLSALLLLLQVSSWLCQEPEPCHPGFDAESYTFTVPRRHLERGRVLGRVNFED CTGRQRTAYFSLDTRFKVGTDGVITVKRPLRFHNPQIHFLVYAWDSTYRKFSTKVTLNT VGHHHRPPPHQASVSGIQAELLTFPNSSPGLRRQKRDWVIPPISCPENEKGPFPKNLVQI KSNKDKEGKVFYSITGQGADTPPVGVFIIERETGWLKVTEPLDRERIATYTLFSHAVSSN GNAVEDPMEILITVTDQNDNKPEFTQEVFKGSVMEGALPGTSVMEVTATDADDDVNT YNAAIAYTILSQDPELPDKNMFTINRNTGVISVVTTGLDRE corresponding to amino acids 1-336 of Q9UII7 (SEQ ID NO: 963), which also corresponds to amino acids 1-336 of HSECADH_P14 (SEQ ID NO: 98), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence VRGQEDPEGVEDKCVLAQSRGQSKILLGQLSVNTVMV (SEQ ID NO: 1119) corresponding to amino acids 337-373 of HSECADH_P14 (SEQ ID NO: 98), wherein said first and second amino acid sequences are contiguous and in a sequential order.

According to preferred embodiments of the present invention, there is provided an isolated polypeptide encoding for a tail of HSECADH_P14 (SEQ ID NO: 98), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence VRGQEDPEGVEDKCVLAQSRGQSKILLGQLSVNTVMV (SEQ ID NO: 1119) in HSECADH_P14 (SEQ ID NO: 98).

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for HSECADH_P14 (SEQ ID NO: 98), comprising a first amino acid sequence being at least 90% homologous to MGPWSRSLSALLLLLQVSSWLCQEPEPCHPGFDAESYTFTVPRRHLERGRVLGRVNFED CTGRQRTAYFSLDTRFKVGTDGVITVKRPLRFHNPQIHFLVYAWDSTYRKFSTKVTLNT VGHHHRPPPHQASVSGIQAELLTFPNSSPGLRRQKRDWVIPPISCPENEKGPFPKNLVQI KSNKDKEGKVFYSITGQGADTPPVGVFIIERETGWLKVTEPLDRERIATYTLFSHAVSSN GNAVEDPMEILITVTDQNDNKPEFTQEVFKGSVMEGALPGTSVMEVTATDADDDVNT YNAAIAYTILSQDPELPDKNMFTINRNTGVISVVTTGLDRE corresponding to amino acids 1-336 of Q9UII8 (SEQ ID NO: 964), which also corresponds to amino acids 1-336 of HSECADH_P14 (SEQ ID NO: 98), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence VRGQEDPEGVEDKCVLAQSRGQSKILLGQLSVNTVMV (SEQ ID NO: 1119) corresponding to amino acids 337-373 of HSECADH_P14 (SEQ ID NO: 98), wherein said first and second amino acid sequences are contiguous and in a sequential order.

According to preferred embodiments of the present invention, there is provided an isolated polypeptide encoding for a tail of HSECADH_P14 (SEQ ID NO: 98), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence VRGQEDPEGVEDKCVLAQSRGQSKILLGQLSVNTVMV (SEQ ID NO: 1119) in HSECADH_P14 (SEQ ID NO: 98).

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for HSECADH_P14 (SEQ ID NO: 98), comprising a first amino acid sequence being at least 90% homologous to MGPWSRSLSALLLLLQVSSWLCQEPEPCHPGFDAESYTFTVPRRHLERGRVLGRVNFED CTGRQRTAYFSLDTRFKVGTDGVITVKRPLRFHNPQIHFLVYAWDSTYRKFSTKVTLNT VGHHHRPPPHQASVSGIQAELLTFPNSSPGLRRQKRDWVIPPISCPENEKGPFPKNLVQI KSNKDKEGKVFYSITGQGADTPPVGVFIIERETGWLKVTEPLDRERIATYTLFSHAVSSN GNAVEDPMEILITVTDQNDNKPEFTQEVFKGSVMEGALPGTSVMEVTATDADDDVNT YNAAIAYTILSQDPELPDKNMFTINRNTGVISVVTTGLDRE corresponding to amino acids 1-336 of CAD1_HUMAN, which also corresponds to amino acids 1-336 of HSECADH_P14 (SEQ ID NO: 98), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence VRGQEDPEGVEDKCVLAQSRGQSKILLGQLSVNTVMV (SEQ ID NO: 1119) corresponding to amino acids 337-373 of HSECADH_P14 (SEQ ID NO: 98), wherein said first and second amino acid sequences are contiguous and in a sequential order.

According to preferred embodiments of the present invention, there is provided an isolated polypeptide encoding for a tail of HSECADH_P14 (SEQ ID NO: 98), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence VRGQEDPEGVEDKCVLAQSRGQSKILLGQLSVNTVMV (SEQ ID NO: 1119) in HSECADH_P14 (SEQ ID NO: 98).

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for HSECADH_P15 (SEQ ID NO: 99), comprising a first amino acid sequence being at least 90% homologous to MGPWSRSLSALLLLLQVSSWLCQEPEPCHPGFDAESYTFTVPRRHLERGRVLGRVNFED CTGRQRTAYFSLDTRFKVGTDGVITVKRPLRFHNPQIHFLVYAWDSTYRKFSTKVTLNT VGHHHRPPPHQASVSGIQAELLTFPNSSPGLRRQKRDWVIPPISCPENEKGPFPKNLVQI KSNKDKEGKVFYSITGQGADTPPVGVFIIERETGWLKVTEPLDRERIATYT corresponding to amino acids 1-229 of Q9UII7 (SEQ ID NO: 963), which also corresponds to amino acids 1-229 of HSECADH_P15 (SEQ ID NO: 99), and a second amino acid sequence VSIS corresponding to amino acids 230-233 of HSECADH_P15 (SEQ ID NO: 99), wherein said first and second amino acid sequences are contiguous and in a sequential order.

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for HSECADH_P15 (SEQ ID NO: 99), comprising a first amino acid sequence being at least 90% homologous to MGPWSRSLSALLLLLQVSSWLCQEPEPCHPGFDAESYTFTVPRRHLERGRVLGRVNFED CTGRQRTAYFSLDTRFKVGTDGVITVKRPLRFHNPQIHFLVYAWDSTYRKFSTKVTLNT VGHHHRPPPHQASVSGIQAELLTFPNSSPGLRRQKRDWVIPPISCPENEKGPFPKNLVQI KSNKDKEGKVFYSITGQGADTPPVGVFIIERETGWLKVTEPLDRERIATYT corresponding to amino acids 1-229 of Q9UII8 (SEQ ID NO: 964), which also corresponds to amino acids 1-229 of HSECADH_P15 (SEQ ID NO: 99), and a second amino acid sequence VSIS corresponding to amino acids 230-233 of HSECADH_P15 (SEQ ID NO: 99), wherein said first and second amino acid sequences are contiguous and in a sequential order.

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for HSECADH_P15 (SEQ ID NO: 99), comprising a first amino acid sequence being at least 90% homologous to MGPWSRSLSALLLLLQVSSWLCQEPEPCHPGFDAESYTFTVPRRHLERGRVLGRVNFED CTGRQRTAYFSLDTRFKVGTDGVITVKRPLRFHNPQIHFLVYAWDSTYRKFSTKVTLNT VGHHHRPPPHQASVSGIQAELLTFPNSSPGLRRQKRDWVIPPISCPENEKGPFPKNLVQI KSNKDKEGKVFYSITGQGADTPPVGVFIIERETGWLKVTEPLDRERIATYT corresponding to amino acids 1-229 of CAD1_HUMAN, which also corresponds to amino acids 1-229 of HSECADH_P15 (SEQ ID NO: 99), and a second amino acid sequence VSIS corresponding to amino acids 230-233 of HSECADH_P15 (SEQ ID NO: 99), wherein said first and second amino acid sequences are contiguous and in a sequential order.

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for T59832_P5 (SEQ ID NO: 778), comprising a first amino acid sequence being at least 90% homologous to MTLSPLLLFLPPLLLLLDVPTAAVQASPLQALDFFGNGPPVNYK corresponding to amino acids 12-55 of GILT_HUMAN, which also corresponds to amino acids 1-44 of T59832_P5 (SEQ ID NO: 778), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence VGTATGRAGWREQAPCRGTRLLLSPQTSQGKTRAPRGRCPCRVPGKTLFSSRRCGHTP SVPFRFRIPHLRGAAASTRLVPPKGSMSAYCVLLGQELGSPFVAQGTSSAAGQGPPACIL AATLDAFIPARAGLACLWDLLGRCPRG (SEQ ID NO: 1120) corresponding to amino acids 45-189 of T59832_P5 (SEQ ID NO: 778), wherein said first and second amino acid sequences are contiguous and in a sequential order.

According to preferred embodiments of the present invention, there is provided an isolated polypeptide encoding for a tail of T59832_P5 (SEQ ID NO: 778), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence VGTATGRAGWREQAPCRGTRLLLSPQTSQGKTRAPRGRCPCRVPGKTLFSSRRCGHTP SVPFRFRIPHLRGAAASTRLVPPKGSMSAYCVLLGQELGSPFVAQGTSSAAGQGPPACIL AATLDAFIPARAGLACLWDLLGRCPRG (SEQ ID NO: 1120) in T59832_P5 (SEQ ID NO: 778).

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for T59832_P7 (SEQ ID NO: 779), comprising a first amino acid sequence being at least 90% homologous to MTLSPLLLFLPPLLLLLDVPTAAVQASPLQALDFFGNGPPVNYKTGNLYLRGPLKKSNA PLVNVTLYYEALCGGCRAFLIRELFPTWLLVMEILNVTLVPYGNAQEQNVSGRWEFKC QHGEEECKFNKVEACVLDELDMELAFLTIVCMEEFEDMERSLPLCLQLYAPGLSPDTIM ECAMGDRGMQLMHANAQRTDALQPPHEYVPWVTVNG corresponding to amino acids 12-223 of GILT_HUMAN, which also corresponds to amino acids 1-212 of T59832_P7 (SEQ ID NO: 779), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence VRIFLALSLTLIVPWSQGWTRQRDQR (SEQ ID NO: 1089) corresponding to amino acids 213-238 of T59832_P7 (SEQ ID NO: 779), wherein said first and second amino acid sequences are contiguous and in a sequential order.

According to preferred embodiments of the present invention, there is provided an isolated polypeptide encoding for a tail of T59832_P7 (SEQ ID NO: 779), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence VRIFLALSLTLIVPWSQGWTRQRDQR (SEQ ID NO: 1089) in T59832_P7 (SEQ ID NO: 779).

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for T59832_P7 (SEQ ID NO: 779), comprising a first amino acid sequence being at least 90% homologous to MTLSPLLLFLPPLLLLLDVPTAAVQASPLQALDFFGNGPPVNYKTGNLYLRGPLKKSNA PLVNVTLYYEALCGGCRAFLIRELFPTWLLVMEILNVTLVPYGNAQEQNVSGRWEFKC QHGEEECKFNKVEACVLDELDMELAFLTIVCMEEFEDMERSLPLCLQLYAPGLSPDTIM ECAMGDRGMQLMHANAQRTDALQPPHEYVPWVTVNG corresponding to amino acids 1-212 of BAC98466, which also corresponds to amino acids 1-212 of T59832_P7 (SEQ ID NO: 779), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence VRIFLALSLTLIVPWSQGWTRQRDQR (SEQ ID NO: 1089) corresponding to amino acids 213-238 of T59832_P7 (SEQ ID NO: 779), wherein said first and second amino acid sequences are contiguous and in a sequential order.

According to preferred embodiments of the present invention, there is provided an isolated polypeptide encoding for a tail of T59832_P7 (SEQ ID NO: 779), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence VRIFLALSLTLIVPWSQGWTRQRDQR (SEQ ID NO: 1089) in T59832_P7 (SEQ ID NO: 779).

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for T59832_P7 (SEQ ID NO: 779), comprising a first amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence MTLSPLLLFLPPLLLLLDVPTAAVQASPLQALDFFGNGPPVNYKTGNLYLRGPLKKSNA PLVNVTLYYEALCGGCRAFLIRELFPTWLLV (SEQ ID NO: 1121) corresponding to amino acids 1-90 of T59832_P7 (SEQ ID NO: 779), and a second amino acid sequence being at least 90% homologous to MEILNVTLVPYGNAQEQNVSGRWEFKCQHGEEECKFNKVEACVLDELDMELAFLTIVC MEEFEDMERSLPLCLQLYAPGLSPDTIMECAMGDRGMQLMHANAQRTDALQPPHEYV PWVTVNGVRIFLALSLTLIVPWSQGWTRQRDQR (SEQ ID NO: 1089) corresponding to amino acids 1-148 of BAC85622, which also corresponds to amino acids 91-238 of T59832_P7 (SEQ ID NO: 779), wherein said first and second amino acid sequences are contiguous and in a sequential order.

According to preferred embodiments of the present invention, there is provided an isolated polypeptide encoding for a head of T59832_P7 (SEQ ID NO: 779), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence MTLSPLLLFLPPLLLLLDVPTAAVQASPLQALDFFGNGPPVNYKTGNLYLRGPLKKSNA PLVNVTLYYEALCGGCRAFLIRELFPTWLLV (SEQ ID NO: 1121) of T59832_P7 (SEQ ID NO: 779).

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for T59832_P7 (SEQ ID NO: 779), comprising a first amino acid sequence being at least 90% homologous to MTLSPLLLFLPPLLLLLDVPTAAVQASPLQALDFFGNGPPVNYKTGNLYLRGPLKKSNA PLVNVTLYYEALCGGCRAFLIRELFPTWLLVMEILNVTLVPYGNAQEQNVSGRWEFKC QHGEEECKFNKVEACVLDELDMELAFLTIVCMEEFEDMERSLPLCLQLYAPGLSPDTIM ECAMGDRGMQLMHANAQRTDALQPPHEYVPWVTVNG corresponding to amino acids 1-212 of Q8WU77, which also corresponds to amino acids 1-212 of T59832_P7 (SEQ ID NO: 779), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence VRIFLALSLTLIVPWSQGWTRQRDQR (SEQ ID NO: 1089) corresponding to amino acids 213-238 of T59832_P7 (SEQ ID NO: 779), wherein said first and second amino acid sequences are contiguous and in a sequential order.

According to preferred embodiments of the present invention, there is provided an isolated polypeptide encoding for a tail of T59832_P7 (SEQ ID NO: 779), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence VRIFLALSLTLIVPWSQGWTRQRDQR (SEQ ID NO: 1089) in T59832_P7 (SEQ ID NO: 779).

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for T59832_P9 (SEQ ID NO: 780), comprising a first amino acid sequence being at least 90% homologous to MTLSPLLLFLPPLLLLLDVPTAAVQASPLQALDFFGNGPPVNYKTGNLYLRGPLKKSNA PLVNVTLYYEALCGGCRAFLIRELFPTWLLVMEILNVTLVPYGNAQEQNVSGRWEFKC QHGEEECKFNKVEACVLDELDMELAFLTIVCMEEFEDMERSLPLCLQLYAPGLSPDTIM ECAMGDRGMQLMHANAQRTDALQPPHE corresponding to amino acids 12-214 of GILT_HUMAN, which also corresponds to amino acids 1-203 of T59832_P9 (SEQ ID NO: 780), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence NPWKIRPSSLPLSASCTRARSRMSALPQPAPSGVFASSDGR (SEQ ID NO: 1090) corresponding to amino acids 204-244 of T59832_P9 (SEQ ID NO: 780), wherein said first and second amino acid sequences are contiguous and in a sequential order.

According to preferred embodiments of the present invention, there is provided an isolated polypeptide encoding for a tail of T59832_P9 (SEQ ID NO: 780), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence NPWKIRPSSLPLSASCTRARSRMSALPQPAPSGVFASSDGR (SEQ ID NO: 1090) in T59832_P9 (SEQ ID NO: 780).

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for T59832_P9 (SEQ ID NO: 780), comprising a first amino acid sequence being at least 90% homologous to MTLSPLLLFLPPLLLLLDVPTAAVQASPLQALDFFGNGPPVNYKTGNLYLRGPLKKSNA PLVNVTLYYEALCGGCRAFLIRELFPTWLLVMEILNVTLVPYGNAQEQNVSGRWEFKC QHGEEECKFNKVEACVLDELDMELAFLTIVCMEEFEDMERSLPLCLQLYAPGLSPDTIM ECAMGDRGMQLMHANAQRTDALQPPHE corresponding to amino acids 1-203 of BAC98466, which also corresponds to amino acids 1-203 of T59832_P9 (SEQ ID NO: 780), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence NPWKIRPSSLPLSASCTRARSRMSALPQPAPSGVFASSDGR (SEQ ID NO: 1090) corresponding to amino acids 204-244 of T59832_P9 (SEQ ID NO: 780), wherein said first and second amino acid sequences are contiguous and in a sequential order.

According to preferred embodiments of the present invention, there is provided an isolated polypeptide encoding for a tail of T59832_P9 (SEQ ID NO: 780), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence NPWKIRPSSLPLSASCTRARSRMSALPQPAPSGVFASSDGR (SEQ ID NO: 1090) in T59832_P9 (SEQ ID NO: 780).

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for T59832_P9 (SEQ ID NO: 780), comprising a first amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence MTLSPLLLFLPPLLLLLDVPTAAVQASPLQALDFFGNGPPVNYKTGNLYLRGPLKKSNA PLVNVTLYYEALCGGCRAFLIRELFPTWLLV (SEQ ID NO: 1121) corresponding to amino acids 1-90 of T59832_P9 (SEQ ID NO: 780), second amino acid sequence being at least 90% homologous to MEILNVTLVPYGNAQEQNVSGRWEFKCQHGEEECKFNKVEACVLDELDMELAFLTIVC MEEFEDMERSLPLCLQLYAPGLSPDTIMECAMGDRGMQLMHANAQRTDALQPPHE corresponding to amino acids 1-113 of BAC85622, which also corresponds to amino acids 91-203 of T59832_P9 (SEQ ID NO: 780), and a third amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence NPWKIRPSSLPLSASCTRARSRMSALPQPAPSGVFASSDGR (SEQ ID NO: 1090) corresponding to amino acids 204-244 of T59832_P9 (SEQ ID NO: 780), wherein said first, second and third amino acid sequences are contiguous and in a sequential order.

According to preferred embodiments of the present invention, there is provided an isolated polypeptide encoding for a head of T59832_P9 (SEQ ID NO: 780), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence MTLSPLLLFLPPLLLLLDVPTAAVQASPLQALDFFGNGPPVNYKTGNLYLRGPLKKSNA PLVNVTLYYEALCGGCRAFLIRELFPTWLLV (SEQ ID NO: 1121) of T59832_P9 (SEQ ID NO: 780).

According to preferred embodiments of the present invention, there is provided an isolated polypeptide encoding for a tail of T59832_P9 (SEQ ID NO: 780), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence NPWKIRPSSLPLSASCTRARSRMSALPQPAPSGVFASSDGR (SEQ ID NO: 1090) in T59832_P9 (SEQ ID NO: 780).

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for T59832_P9 (SEQ ID NO: 780), comprising a first amino acid sequence being at least 90% homologous to MTLSPLLLFLPPLLLLLDVPTAAVQASPLQALDFFGNGPPVNYKTGNLYLRGPLKKSNA PLVNVTLYYEALCGGCRAFLIRELFPTWLLVMEILNVTLVPYGNAQEQNVSGRWEFKC QHGEEECKFNKVEACVLDELDMELAFLTIVCMEEFEDMERSLPLCLQLYAPGLSPDTIM ECAMGDRGMQLMHANAQRTDALQPPHE corresponding to amino acids 1-203 of Q8WU77, which also corresponds to amino acids 1-203 of T59832_P9 (SEQ ID NO: 780), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence NPWKIRPSSLPLSASCTRARSRMSALPQPAPSGVFASSDGR (SEQ ID NO: 1090) corresponding to amino acids 204-244 of T59832_P9 (SEQ ID NO: 780), wherein said first and second amino acid sequences are contiguous and in a sequential order.

According to preferred embodiments of the present invention, there is provided an isolated polypeptide encoding for a tail of T59832_P9 (SEQ ID NO: 780), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence NPWKIRPSSLPLSASCTRARSRMSALPQPAPSGVFASSDGR (SEQ ID NO: 1090) in T59832_P9 (SEQ ID NO: 780).

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for T59832_P12 (SEQ ID NO: 781), comprising a first amino acid sequence being at least 90% homologous to MTLSPLLLFLPPLLLLLDVPTAAVQASPLQALDFFGNGPPVNYKTGNLYLRGPLKKSNA PLVNVTLYYEALCGGCRAFLIRELFPTWLLVMEILNVTLVPYGNAQEQNVSGRWEFKC QHGEEECKFNKVE corresponding to amino acids 12-141 of GILT_HUMAN, which also corresponds to amino acids 1-130 of T59832_P12 (SEQ ID NO: 781), and a second amino acid sequence being at least 90% homologous to CLQLYAPGLSPDTIMECAMGDRGMQLMHANAQRTDALQPPHEYVPWVTVNGKPLED QTQLLTLVCQLYQGKKPDVCPSSTSSLRSVCFK corresponding to amino acids 173-261 of GILT_HUMAN, which also corresponds to amino acids 131-219 of T59832_P12 (SEQ ID NO: 781), wherein said first and second amino acid sequences are contiguous and in a sequential order.

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for an edge portion of T59832_P12 (SEQ ID NO: 781), comprising a polypeptide having a length “n”, wherein n is at least about 10 amino acids in length, optionally at least about 20 amino acids in length, preferably at least about 30 amino acids in length, more preferably at least about 40 amino acids in length and most preferably at least about 50 amino acids in length, wherein at least two amino acids comprise EC, having a structure as follows: a sequence starting from any of amino acid numbers 130-x to 130; and ending at any of amino acid numbers 131+((n−2)−x), in which x varies from 0 to n−2.

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for T59832_P12 (SEQ ID NO: 781), comprising a first amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence MTLSPLLLFLPPLLLLLDVPTAAVQASPLQALDFFGNGPPVNYKTGNLYLRGPLKKSNA PLVNVTLYYEALCGGCRAFLIRELFPTWLLV (SEQ ID NO: 1121) corresponding to amino acids 1-90 of T59832_P12 (SEQ ID NO: 781), second amino acid sequence being at least 90% homologous to MEILNVTLVPYGNAQEQNVSGRWEFKCQHGEEECKFNKVE corresponding to amino acids 1-40 of BAC85622, which also corresponds to amino acids 91-130 of T59832_P12 (SEQ ID NO: 781), third amino acid sequence being at least 90% homologous to CLQLYAPGLSPDTIMECAMGDRGMQLMHANAQRTDALQPPHEYVPWVTVNG corresponding to amino acids 72-122 of BAC85622, which also corresponds to amino acids 131-181 of T59832_P12 (SEQ ID NO: 781), and a fourth amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence KPLEDQTQLLTLVCQLYQGKKPDVCPSSTSSLRSVCFK (SEQ ID NO: 1124) corresponding to amino acids 182-219 of T59832_P12 (SEQ ID NO: 781), wherein said first, second, third and fourth amino acid sequences are contiguous and in a sequential order.

According to preferred embodiments of the present invention, there is provided an isolated polypeptide encoding for a head of T59832_P12 (SEQ ID NO: 781), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence MTLSPLLLFLPPLLLLLDVPTAAVQASPLQALDFFGNGPPVNYKTGNLYLRGPLKKSNA PLVNVTLYYEALCGGCRAFLIRELFPTWLLV (SEQ ID NO: 1121) of T59832_P12 (SEQ ID NO: 781).

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for an edge portion of T59832_P12 (SEQ ID NO: 781), comprising a polypeptide having a length “n”, wherein n is at least about 10 amino acids in length, optionally at least about 20 amino acids in length, preferably at least about 30 amino acids in length, more preferably at least about 40 amino acids in length and most preferably at least about 50 amino acids in length, wherein at least two amino acids comprise EC, having a structure as follows: a sequence starting from any of amino acid numbers 130-x to 130; and ending at any of amino acid numbers 131+((n−2)−x), in which x varies from 0 to n−2.

According to preferred embodiments of the present invention, there is provided an isolated polypeptide encoding for a tail of T59832_P12 (SEQ ID NO: 781), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence KPLEDQTQLLTLVCQLYQGKKPDVCPSSTSSLRSVCFK (SEQ ID NO: 1124) in T59832_P12 (SEQ ID NO: 781).

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for T59832_P12 (SEQ ID NO: 781), comprising a first amino acid sequence being at least 90% homologous to MTLSPLLLFLPPLLLLLDVPTAAVQASPLQALDFFGNGPPVNYKTGNLYLRGPLKKSNA PLVNVTLYYEALCGGCRAFLIRELFPTWLLVMEILNVTLVPYGNAQEQNVSGRWEFKC QHGEEECKFNKVE corresponding to amino acids 1-130 of Q8WU77, which also corresponds to amino acids 1-130 of T59832_P12 (SEQ ID NO: 781), and a second amino acid sequence being at least 90% homologous to CLQLYAPGLSPDTIMECAMGDRGMQLMHANAQRTDALQPPHEYVPWVTVNGKPLED QTQLLTLVCQLYQGKKPDVCPSSTSSLRSVCFK corresponding to amino acids 162-250 of Q8WU77, which also corresponds to amino acids 131-219 of T59832_P12 (SEQ ID NO: 781), wherein said first and second amino acid sequences are contiguous and in a sequential order.

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for an edge portion of T59832_P12 (SEQ ID NO: 781), comprising a polypeptide having a length “n”, wherein n is at least about 10 amino acids in length, optionally at least about 20 amino acids in length, preferably at least about 30 amino acids in length, more preferably at least about 40 amino acids in length and most preferably at least about 50 amino acids in length, wherein at least two amino acids comprise EC, having a structure as follows: a sequence starting from any of amino acid numbers 130-x to 130; and ending at any of amino acid numbers 131+((n−2)−x), in which x varies from 0 to n−2.

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for T59832_P18 (SEQ ID NO: 782), comprising a first amino acid sequence being at least 90% homologous to MTLSPLLLFLPPLLLLLDVPTAAVQASPLQALDFFGNGPPVNYK corresponding to amino acids 12-55 of GILT_HUMAN, which also corresponds to amino acids 1-44 of T59832_P18 (SEQ ID NO: 782), and a second amino acid sequence being at least 90% homologous to CLQLYAPGLSPDTIMECAMGDRGMQLMHANAQRTDALQPPHEYVPWVTVNGKPLED QTQLLTLVCQLYQGKKPDVCPSSTSSLRSVCFK corresponding to amino acids 173-261 of GILT_HUMAN, which also corresponds to amino acids 45-133 of T59832_P18 (SEQ ID NO: 782), wherein said first and second amino acid sequences are contiguous and in a sequential order.

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for an edge portion of T59832_P18 (SEQ ID NO: 782), comprising a polypeptide having a length “n”, wherein n is at least about 10 amino acids in length, optionally at least about 20 amino acids in length, preferably at least about 30 amino acids in length, more preferably at least about 40 amino acids in length and most preferably at least about 50 amino acids in length, wherein at least two amino acids comprise KC, having a structure as follows: a sequence starting from any of amino acid numbers 44-x to 44; and ending at any of amino acid numbers 45+((n−2)−x), in which x varies from 0 to n−2.

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for T59832_P18 (SEQ ID NO: 782), comprising a first amino acid sequence being at least 90% homologous to MTLSPLLLFLPPLLLLLDVPTAAVQASPLQALDFFGNGPPVNYK corresponding to amino acids 1-44 of Q8WU77, which also corresponds to amino acids 1-44 of T59832_P18 (SEQ ID NO: 782), and a second amino acid sequence being at least 90% homologous to CLQLYAPGLSPDTIMECAMGDRGMQLMHANAQRTDALQPPHEYVPWVTVNGKPLED QTQLLTLVCQLYQGKKPDVCPSSTSSLRSVCFK corresponding to amino acids 162-250 of Q8WU77, which also corresponds to amino acids 45-133 of T59832_P18 (SEQ ID NO: 782), wherein said first and second amino acid sequences are contiguous and in a sequential order.

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for an edge portion of T59832_P18 (SEQ ID NO: 782), comprising a polypeptide having a length “n”, wherein n is at least about 10 amino acids in length, optionally at least about 20 amino acids in length, preferably at least about 30 amino acids in length, more preferably at least about 40 amino acids in length and most preferably at least about 50 amino acids in length, wherein at least two amino acids comprise KC, having a structure as follows: a sequence starting from any of amino acid numbers 44-x to 44; and ending at any of amino acid numbers 45+((n−2)−x), in which x varies from 0 to n−2.

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for T59832_P18 (SEQ ID NO: 782), comprising a first amino acid sequence being at least 90% homologous to MTLSPLLLFLPPLLLLLDVPTAAVQASPLQALDFFGNGPPVNYK corresponding to amino acids 1-44 of Q8NEI4, which also corresponds to amino acids 1-44 of T59832_P18 (SEQ ID NO: 782), and a second amino acid sequence being at least 90% homologous to CLQLYAPGLSPDTIMECAMGDRGMQLMHANAQRTDALQPPHEYVPWVTVNGKPLED QTQLLTLVCQLYQGKKPDVCPSSTSSLRSVCFK corresponding to amino acids 162-250 of Q8NEI4, which also corresponds to amino acids 45-133 of T59832_P18 (SEQ ID NO: 782), wherein said first and second amino acid sequences are contiguous and in a sequential order.

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for an edge portion of T59832_P18 (SEQ ID NO: 782), comprising a polypeptide having a length “n”, wherein n is at least about 10 amino acids in length, optionally at least about 20 amino acids in length, preferably at least about 30 amino acids in length, more preferably at least about 40 amino acids in length and most preferably at least about 50 amino acids in length, wherein at least two amino acids comprise KC, having a structure as follows: a sequence starting from any of amino acid numbers 44-x to 44; and ending at any of amino acid numbers 45+((n−2)−x), in which x varies from 0 to n−2.

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for HUMGRP5E_P4 (SEQ ID NO: 108), comprising a first amino acid sequence being at least 90% homologous to MRGSELPLVLLALVLCLAPRGRAVPLPAGGGTVLTKMYPRGNHWAVGHLMGKKSTG ESSSVSERGSLKQQLREYIRWEEAARNLLGLIEAKENRNHQPPQPKALGNQQPSWDSED SSNFKDVGSKGK corresponding to amino acids 1-127 of GRP_HUMAN, which also corresponds to amino acids 1-127 of HUMGRP5E_P4 (SEQ ID NO: 108), and a second amino acid sequence being at least 90% homologous to GSQREGRNPQLNQQ corresponding to amino acids 135-148 of GRP_HUMAN, which also corresponds to amino acids 128-141 of HUMGRP5E P4 (SEQ ID NO: 108), wherein said first and second amino acid sequences are contiguous and in a sequential order.

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for an edge portion of HUMGRP5E_P4 (SEQ ID NO: 108), comprising a polypeptide having a length “n”, wherein n is at least about 10 amino acids in length, optionally at least about 20 amino acids in length, preferably at least about 30 amino acids in length, more preferably at least about 40 amino acids in length and most preferably at least about 50 amino acids in length, wherein at least two amino acids comprise KG, having a structure as follows: a sequence starting from any of amino acid numbers 127-x to 127; and ending at any of amino acid numbers 128+((n−2)−x), in which x varies from 0 to n−2.

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for HUMGRP5E_P5 (SEQ ID NO: 109), comprising a first amino acid sequence being at least 90% homologous to MRGSELPLVLLALVLCLAPRGRAVPLPAGGGTVLTKMYPRGNHWAVGHLMGKKSTG ESSSVSERGSLKQQLREYIRWEEAARNLLGLIEAKENRNHQPPQPKALGNQQPSWDSED SSNFKDVGSKGK corresponding to amino acids 1-127 of GRP_HUMAN, which also corresponds to amino acids 1-127 of HUMGRP5E_P5 (SEQ ID NO: 109), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence DSLLQVLNVKEGTPS (SEQ ID NO: 1125) corresponding to amino acids 128-142 of HUMGRP5E_P5 (SEQ ID NO: 109), wherein said first and second amino acid sequences are contiguous and in a sequential order.

According to preferred embodiments of the present invention, there is provided an isolated polypeptide encoding for a tail of HUMGRP5E_P5 (SEQ ID NO: 109), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence DSLLQVLNVKEGTPS (SEQ ID NO: 1125) in HUMGRP5E_P5 (SEQ ID NO: 109).

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for R11723_PEA_(—)1_P6 (SEQ ID NO: 143), comprising a first amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence MWVLGIAATFCGLFLLPGFALQIQCYQCEEFQLNNDCSSPEFIVNCTVNVQDMCQKEV MEQSAGIMYRKSCASSAACLIASAGSPCRGLAPGREEQRALHKAGAVGGGVR (SEQ ID NO: 1126) corresponding to amino acids 1-110 of R11723_PEA_(—)1_P6 (SEQ ID NO: 143), and a second amino acid sequence being at least 90% homologous to MYAQALLVVGVLQRQAAAQHLHEHPPKLLRGHRVQERVDDRAEVEKRLREGEEDHV RPEVGPRPVVLGFGRSHDPPNLVGHPAYGQCHNNQPWADTSRRERQRKEKHSMRTQ corresponding to amino acids 1-112 of Q8IXM0 (SEQ ID NO: 968), which also corresponds to amino acids 111-222 of R11723_PEA_(—)1_P6 (SEQ ID NO: 143), wherein said first and second amino acid sequences are contiguous and in a sequential order.

According to preferred embodiments of the present invention, there is provided an isolated polypeptide encoding for a head of R11723_PEA_(—)1_P6 (SEQ ID NO: 143), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence MWVLGIAATFCGLFLLPGFALQIQCYQCEEFQLNNDCSSPEFIVNCTVNVQDMCQKEV MEQSAGIMYRKSCASSAACLIASAGSPCRGLAPGREEQRALHKAGAVGGGVR (SEQ ID NO: 1126) of R11723_PEA_(—)1_P6 (SEQ ID NO: 143).

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for R11723_PEA_(—)1_P6 (SEQ ID NO: 143), comprising a first amino acid sequence being at least 90% homologous to MWVLGIAATFCGLFLLPGFALQIQCYQCEEFQLNNDCSSPEFIVNCTVNVQDMCQKEV MEQSAGIMYRKSCASSAACLIASAG corresponding to amino acids 1-83 of Q96AC2 (SEQ ID NO: 969), which also corresponds to amino acids 1-83 of R11723_PEA_(—)1_P6 (SEQ ID NO: 143), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence SPCRGLAPGREEQRALHKAGAVGGGVRMYAQALLVVGVLQRQAAAQHLHEHPPKLL RGHRVQERVDDRAEVEKRLREGEEDHVRPEVGPRPVVLGFGRSHDPPNLVGHPAYGQ CHNNQPWADTSRRERQRKEKHSMRTQ (SEQ ID NO: 1127) corresponding to amino acids 84-222 of R11723_PEA_(—)1_P6 (SEQ ID NO: 143), wherein said first and second amino acid sequences are contiguous and in a sequential order.

According to preferred embodiments of the present invention, there is provided an isolated polypeptide encoding for a tail of R11723_PEA_(—)1_P6 (SEQ ID NO: 143), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence SPCRGLAPGREEQRALHKAGAVGGGVRMYAQALLVVGVLQRQAAAQHLHEHPPKLL RGHRVQERVDDRAEVEKRLREGEEDHVRPEVGPRPVVLGFGRSHDPPNLVGHPAYGQ CHNNQPWADTSRRERQRKEKHSMRTQ (SEQ ID NO: 1127) in R11723_PEA_(—)1_P6 (SEQ ID NO: 143).

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for R11723_PEA_(—)1_P6 (SEQ ID NO: 143), comprising a first amino acid sequence being at least 90% homologous to MWVLGIAATFCGLFLLPGFALQIQCYQCEEFQLNNDCSSPEFIVNCTVNVQDMCQKEV MEQSAGIMYRKSCASSAACLIASAG corresponding to amino acids 1-83 of Q8N2G4 (SEQ ID NO: 970), which also corresponds to amino acids 1-83 of R11723_PEA_(—)1_P6 (SEQ ID NO: 143), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence SPCRGLAPGREEQRALHKAGAVGGGVRMYAQALLVVGVLQRQAAAQHLHEHPPKLL RGHRVQERVDDRAEVEKRLREGEEDHVRPEVGPRPVVLGFGRSHDPPNLVGHPAYGQ CHNNQPWADTSRRERQRKEKHSMRTQ (SEQ ID NO: 1127) corresponding to amino acids 84-222 of R11723_PEA_(—)1_P6 (SEQ ID NO: 143), wherein said first and second amino acid sequences are contiguous and in a sequential order.

According to preferred embodiments of the present invention, there is provided an isolated polypeptide encoding for a tail of R11723_PEA_(—)1_P6 (SEQ ID NO: 143), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence SPCRGLAPGREEQRALHKAGAVGGGVRMYAQALLVVGVLQRQAAAQHLHEHPPKLL RGHRVQERVDDRAEVEKRLREGEEDHVRPEVGPRPVVLGFGRSHDPPNLVGHPAYGQ CHNNQPWADTSRRERQRKEKHSMRTQ (SEQ ID NO: 1127) in R11723_PEA_(—)1_P6 (SEQ ID NO: 143).

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for R11723_PEA_(—)1_P6 (SEQ ID NO: 143), comprising a first amino acid sequence being at least 90% homologous to MWVLGIAATFCGLFLLPGFALQIQCYQCEEFQLNNDCSSPEFIVNCTVNVQDMCQKEV MEQSAGIMYRKSCASSAACLIASAG corresponding to amino acids 24-106 of BAC85518 (SEQ ID NO: 971), which also corresponds to amino acids 1-83 of R11723_PEA_(—)1_P6 (SEQ ID NO: 143), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence SPCRGLAPGREEQRALHKAGAVGGGVRMYAQALLVVGVLQRQAAAQHLHEHPPKLL RGHRVQERVDDRAEVEKRLREGEEDHVRPEVGPRPVVLGFGRSHDPPNLVGHPAYGQ CHNNQPWADTSRRERQRKEKHSMRTQ (SEQ ID NO: 1127) corresponding to amino acids 84-222 of R11723_PEA_(—)1_P6 (SEQ ID NO: 143), wherein said first and second amino acid sequences are contiguous and in a sequential order.

According to preferred embodiments of the present invention, there is provided an isolated polypeptide encoding for a tail of R11723_PEA_(—)1_P6 (SEQ ID NO: 143), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence SPCRGLAPGREEQRALHKAGAVGGGVRMYAQALLVVGVLQRQAAAQHLHEHPPKLL RGHRVQERVDDRAEVEKRLREGEEDHVRPEVGPRPVVLGFGRSHDPPNLVGHPAYGQ CHNNQPWADTSRRERQRKEKHSMRTQ (SEQ ID NO: 1127) in R11723_PEA_(—)1_P6 (SEQ ID NO: 143).

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for R11723_PEA_(—)1_P7 (SEQ ID NO: 144), comprising a first amino acid sequence being at least 90% homologous to MWVLGIAATFCGLFLLPGFALQIQCYQCEEFQLNNDCSSPEFIVNCTVNVQDMCQKEV MEQSAG corresponding to amino acids 1-64 of Q96AC2 (SEQ ID NO: 969), which also corresponds to amino acids 1-64 of R11723_PEA_(—)1_P7 (SEQ ID NO: 144), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence SHCVTRLECSGTISAHCNLCLPGSNDHPT (SEQ ID NO: 1128) corresponding to amino acids 65-93 of R11723_PEA_(—)1_P7 (SEQ ID NO: 144), wherein said first and second amino acid sequences are contiguous and in a sequential order.

According to preferred embodiments of the present invention, there is provided an isolated polypeptide encoding for a tail of R11723_PEA_(—)1_P7 (SEQ ID NO: 144), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence SHCVTRLECSGTISAHCNLCLPGSNDHPT (SEQ ID NO: 1128) in R11723_PEA_(—)1_P7 (SEQ ID NO: 144).

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for R11723_PEA_(—)1_P7 (SEQ ID NO: 144), comprising a first amino acid sequence being at least 90% homologous to MWVLGIAATFCGLFLLPGFALQIQCYQCEEFQLNNDCSSPEFIVNCTVNVQDMCQKEV MEQSAG corresponding to amino acids 1-64 of Q8N2G4 (SEQ ID NO: 970), which also corresponds to amino acids 1-64 of R11723_PEA_(—)1_P7 (SEQ ID NO: 144), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence SHCVTRLECSGTISAHCNLCLPGSNDHPT (SEQ ID NO: 1128) corresponding to amino acids 65-93 of R11723_PEA_(—)1_P7 (SEQ ID NO: 144), wherein said first and second amino acid sequences are contiguous and in a sequential order.

According to preferred embodiments of the present invention, there is provided an isolated polypeptide encoding for a tail of R11723_PEA_(—)1_P7 (SEQ ID NO: 144), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence SHCVTRLECSGTISAHCNLCLPGSNDHPT (SEQ ID NO: 1128) in R11723_PEA_(—)1_P7 (SEQ ID NO: 144).

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for R11723_PEA_(—)1_P7 (SEQ ID NO: 144), comprising a first amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence MWVLG (SEQ ID NO: 1129) corresponding to amino acids 1-5 of R11723_PEA_(—)1_P7 (SEQ ID NO: 144), second amino acid sequence being at least 90% homologous to IAATFCGLFLLPGFALQIQCYQCEEFQLNNDCSSPEFIVNCTVNVQDMCQKEVMEQSAG corresponding to amino acids 22-80 of BAC85273 (SEQ ID NO: 972), which also corresponds to amino acids 6-64 of R11723_PEA_(—)1_P7 (SEQ ID NO: 144), and a third amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence SHCVTRLECSGTISAHCNLCLPGSNDHPT (SEQ ID NO: 1128) corresponding to amino acids 65-93 of R11723_PEA_(—)1_P7 (SEQ ID NO: 144), wherein said first, second and third amino acid sequences are contiguous and in a sequential order.

According to preferred embodiments of the present invention, there is provided an isolated polypeptide encoding for a head of R11723_PEA_(—)1_P7 (SEQ ID NO: 144), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence MWVLG (SEQ ID NO: 1129) of R11723_PEA_(—)1_P7 (SEQ ID NO: 144).

According to preferred embodiments of the present invention, there is provided an isolated polypeptide encoding for a tail of R11723_PEA_(—)1_P7 (SEQ ID NO: 144), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence SHCVTRLECSGTISAHCNLCLPGSNDHPT (SEQ ID NO: 1128) in R11723_PEA_(—)1_P7 (SEQ ID NO: 144).

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for R11723_PEA_(—)1_P7 (SEQ ID NO: 144), comprising a first amino acid sequence being at least 90% homologous to MWVLGIAATFCGLFLLPGFALQIQCYQCEEFQLNNDCSSPEFIVNCTVNVQDMCQKEV MEQSAG corresponding to amino acids 24-87 of BAC85518 (SEQ ID NO: 971), which also corresponds to amino acids 1-64 of R11723_PEA_(—)1_P7 (SEQ ID NO: 144), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence SHCVTRLECSGTISAHCNLCLPGSNDHPT (SEQ ID NO: 1128) corresponding to amino acids 65-93 of R11723_PEA_(—)1_P7 (SEQ ID NO: 144), wherein said first and second amino acid sequences are contiguous and in a sequential order.

According to preferred embodiments of the present invention, there is provided an isolated polypeptide encoding for a tail of R11723_PEA_(—)1_P7 (SEQ ID NO: 144), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence SHCVTRLECSGTISAHCNLCLPGSNDHPT (SEQ ID NO: 1128) in R11723_PEA_(—)1_P7 (SEQ ID NO: 144).

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for R11723_PEA_(—)1_P13 (SEQ ID NO: 145), comprising a first amino acid sequence being at least 90% homologous to MWVLGIAATFCGLFLLPGFALQIQCYQCEEFQLNNDCSSPEFIVNCTVNVQDMCQKEV MEQSA corresponding to amino acids 1-63 of Q96AC2 (SEQ ID NO: 969), which also corresponds to amino acids 1-63 of R11723_PEA_(—)1_P13 (SEQ ID NO: 145), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence DTKRTNTLLFEMRHFAKQLTT (SEQ ID NO: 1130) corresponding to amino acids 64-84 of R11723_PEA_(—)1_P13 (SEQ ID NO: 145), wherein said first and second amino acid sequences are contiguous and in a sequential order.

According to preferred embodiments of the present invention, there is provided an isolated polypeptide encoding for a tail of R11723_PEA_(—)1_P13 (SEQ ID NO: 145), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence DTKRTNTLLFEMRHFAKQLTT (SEQ ID NO: 1130) in R11723_PEA_(—)1_P13 (SEQ ID NO: 145).

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for R11723_PEA_(—)1_P10 (SEQ ID NO: 146), comprising a first amino acid sequence being at least 90% homologous to MWVLGIAATFCGLFLLPGFALQIQCYQCEEFQLNNDCSSPEFIVNCTVNVQDMCQKEV MEQSA corresponding to amino acids 1-63 of Q96AC2 (SEQ ID NO: 969), which also corresponds to amino acids 1-63 of R11723_PEA_(—)1_P10 (SEQ ID NO: 146), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence DRVSLCHEAGVQWNNFSTLQPLPPRLK (SEQ ID NO: 1131) corresponding to amino acids 64-90 of R11723_PEA_(—)1_P10 (SEQ ID NO: 146), wherein said first and second amino acid sequences are contiguous and in a sequential order.

According to preferred embodiments of the present invention, there is provided an isolated polypeptide encoding for a tail of R11723_PEA_(—)1_P10 (SEQ ID NO: 146), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence DRVSLCHEAGVQWNNFSTLQPLPPRLK (SEQ ID NO: 1131) in R11723_PEA_(—)1_P10 (SEQ ID NO: 146).

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for R11723_PEA_(—)1_P10 (SEQ ID NO: 146), comprising a first amino acid sequence being at least 90% homologous to MWVLGIAATFCGLFLLPGFALQIQCYQCEEFQLNNDCSSPEFIVNCTVNVQDMCQKEV MEQSA corresponding to amino acids 1-63 of Q8N2G4 (SEQ ID NO: 970), which also corresponds to amino acids 1-63 of R11723_PEA_(—)1_P10 (SEQ ID NO: 146), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence DRVSLCHEAGVQWNNFSTLQPLPPRLK (SEQ ID NO: 1131) corresponding to amino acids 64-90 of R11723_PEA_(—)1_P10 (SEQ ID NO: 146), wherein said first and second amino acid sequences are contiguous and in a sequential order.

According to preferred embodiments of the present invention, there is provided an isolated polypeptide encoding for a tail of R11723_PEA_(—)1_P10 (SEQ ID NO: 146), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence DRVSLCHEAGVQWNNFSTLQPLPPRLK (SEQ ID NO: 1131) in R11723_PEA_(—)1_P10(SEQ ID NO:146).

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for R11723_PEA_(—)1_P10 (SEQ ID NO: 146), comprising a first amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence MWVLG (SEQ ID NO: 1129) corresponding to amino acids 1-5 of R11723_PEA_(—)1_P10 (SEQ ID NO: 146), second amino acid sequence being at least 90% homologous to IAATFCGLFLLPGFALQIQCYQCEEFQLNNDCSSPEFIVNCTVNVQDMCQKEVMEQSA corresponding to amino acids 22-79 of BAC85273 (SEQ ID NO: 972), which also corresponds to amino acids 6-63 of R11723_PEA_(—)1_P10 (SEQ ID NO: 146), and a third amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence DRVSLCHEAGVQWNNFSTLQPLPPRLK (SEQ ID NO: 1131) corresponding to amino acids 64-90 of R11723_PEA_(—)1_P10 (SEQ ID NO: 146), wherein said first, second and third amino acid sequences are contiguous and in a sequential order.

According to preferred embodiments of the present invention, there is provided an isolated polypeptide encoding for a head of R11723_PEA_(—)1_P10 (SEQ ID NO: 146), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence MWVLG (SEQ ID NO: 1129) of R11723_PEA_(—)1_P10 (SEQ ID NO: 146).

According to preferred embodiments of the present invention, there is provided an isolated polypeptide encoding for a tail of R11723_PEA_(—)1_P10 (SEQ ID NO: 146), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence DRVSLCHEAGVQWNNFSTLQPLPPRLK (SEQ ID NO: 1131) in R11723_PEA_(—)1_P10 (SEQ ID NO: 146).

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for R11723_PEA_(—)1_P10 (SEQ ID NO: 146), comprising a first amino acid sequence being at least 90% homologous to MWVLGIAATFCGLFLLPGFALQIQCYQCEEFQLNNDCSSPEFIVNCTVNVQDMCQKEV MEQSA corresponding to amino acids 24-86 of BAC85518 (SEQ ID NO: 971), which also corresponds to amino acids 1-63 of R11723_PEA_(—)1_P10 (SEQ ID NO: 146), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence DRVSLCHEAGVQWNNFSTLQPLPPRLK (SEQ ID NO: 1131) corresponding to amino acids 64-90 of R11723_PEA_(—)1_P10 (SEQ ID NO: 146), wherein said first and second amino acid sequences are contiguous and in a sequential order.

According to preferred embodiments of the present invention, there is provided an isolated polypeptide encoding for a tail of R11723_PEA_(—)1_P10 (SEQ ID NO: 146), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence DRVSLCHEAGVQWNNFSTLQPLPPRLK (SEQ ID NO: 1131) in R11723_PEA_(—)1_P10 (SEQ ID NO: 146).

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for D56406_PEA_(—)1_P2 (SEQ ID NO: 161), comprising a first amino acid sequence being at least 90% homologous to MMAGMKIQLVCMLLLAFSSWSLCSDSEEEMKALEADFLTNMHTSKISKAHVPSWKMT LLNVCSLVNNLNSPAEETGEVHEEELVARRKLPTALDGFSLEAMLTIYQLHKICHSRAF QHWE corresponding to amino acids 1-120 of NEUT_HUMAN, which also corresponds to amino acids 1-120 of D56406_PEA_(—)1_P2 (SEQ ID NO: 161), second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence ARWLTPVIPALWEAETGGSRGQEMETIPANT (SEQ ID NO: 1141) corresponding to amino acids 121-151 of D56406_PEA_(—)1_P2 (SEQ ID NO: 161), and a third amino acid sequence being at least 90% homologous to LIQEDILDTGNDKNGKEEVIKRKIPYILKRQLYENKPRRPYILKRDSYYY corresponding to amino acids 121-170 of NEUT_HUMAN, which also corresponds to amino acids 152-201 of D56406_PEA_(—)1_P2 (SEQ ID NO: 161), wherein said first, second and third amino acid sequences are contiguous and in a sequential order.

According to preferred embodiments of the present invention, there is provided an isolated polypeptide encoding for an edge portion of D56406_PEA_(—)1_P2 (SEQ ID NO: 161),

comprising an amino acid sequence being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence encoding for ARWLTPVIPALWEAETGGSRGQEMETIPANT (SEQ ID NO: 1141), corresponding to D56406_PEA_(—)1_P2 (SEQ ID NO: 161).

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for D56406_PEA_(—)1_P5 (SEQ ID NO: 162), comprising a first amino acid sequence being at least 90% homologous to MMAGMKIQLVCMLLLAFSSWSLC corresponding to amino acids 1-23 of NEUT_HUMAN, which also corresponds to amino acids 1-23 of D56406_PEA_(—)1_P5 (SEQ ID NO: 162), and a second amino acid sequence being at least 90% homologous to SEEEMKALEADFLTNMHTSKISKAHVPSWKMTLLNVCSLVNNLNSPAEETGEVHEEEL VARRKLPTALDGFSLEAMLTIYQLHKICHSRAFQHWELIQEDILDTGNDKNGKEEVIKR KIPYILKRQLYENKPRRPYILKRDSYYY corresponding to amino acids 26-170 of NEUT_HUMAN, which also corresponds to amino acids 24-168 of D56406_PEA_(—)1_P5 (SEQ ID NO: 162), wherein said first and second amino acid sequences are contiguous and in a sequential order.

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for an edge portion of D56406_PEA_(—)1_P5 (SEQ ID NO: 162), comprising a polypeptide having a length “n”, wherein n is at least about 10 amino acids in length, optionally at least about 20 amino acids in length, preferably at least about 30 amino acids in length, more preferably at least about 40 amino acids in length and most preferably at least about 50 amino acids in length, wherein at least two amino acids comprise CS, having a structure as follows: a sequence starting from any of amino acid numbers 23-x to 24; and ending at any of amino acid numbers+((n−2)−x), in which x varies from 0 to n−2.

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for D56406_PEA_(—)1_P6 (SEQ ID NO: 163), comprising a first amino acid sequence being at least 90% homologous to MMAGMKIQLVCMLLLAFSSWSLCSDSEEEMKALEADFLTNMHTSK corresponding to amino acids 1-45 of NEUT_HUMAN, which also corresponds to amino acids 1-45 of D56406_PEA_(—)1_P6 (SEQ ID NO: 163), and a second amino acid sequence being at least 90% homologous to LIQEDILDTGNDKNGKEEVIKRKIPYILKRQLYENKPRRPYILKRDSYYY corresponding to amino acids 121-170 of NEUT_HUMAN, which also corresponds to amino acids 46-95 of D56406_PEA_(—)1_P6 (SEQ ID NO: 163), wherein said first and second amino acid sequences are contiguous and in a sequential order.

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for an edge portion of D56406_PEA_(—)1_P6 (SEQ ID NO: 163), comprising a polypeptide having a length “n”, wherein n is at least about 10 amino acids in length, optionally at least about 20 amino acids in length, preferably at least about 30 amino acids in length, more preferably at least about 40 amino acids in length and most preferably at least about 50 amino acids in length, wherein at least two amino acids comprise KL, having a structure as follows: a sequence starting from any of amino acid numbers 45-x to 46; and ending at any of amino acid numbers 46+((n−2)−x), in which x varies from 0 to n−2.

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for H53393_PEA_(—)1_P2 (SEQ ID NO: 185), comprising a first amino acid sequence being at least 90% homologous to MRTYRYFLLLFWVGQPYPTLSTPLSKRTSGFPAKKRALELSGNSKNELNRSKRSWMWN QFFLLEEYTGSDYQYVGKLHSDQDRGDGSLKYILSGDGAGDLFIINENTGDIQATKRLD REEKPVYILRAQAINRRTGRPVEPESEFIIKIHDINDNEPIFTKEVYTATVPEMSDVGTFVV QVTATDADDPTYGNSAKVVYSILQGQPYFSVESETGIIKTALLNMDRENREQYQVVIQA KDMGGQMGGLSGTTTVNITLTDVNDNPPRFPQSTYQFKTPESSPPGTPIGRIKASDADV GENAEIEYSITDGEGLDMFDVITDQETQEGIITVKKLLDFEKKKVYTLKVEASNPYVEPR FLYLGPFKDSATVRIVVEDVDEPPVFSKLAYILQIREDAQINTTIGSVTAQDPDAARNPV KYSVDRHTDMDRIFNIDSGNGSIFTSKLLDRETLLWHNITVIATEINNPKQSSRVPLYIKV LDVNDNAPEFAEFYETFVCEKAKADQLIQTLHAVDKDDPYSGHQFSFSLAPEAASGSNF TIQDNK corresponding to amino acids 1-543 of CAD6_HUMAN, which also corresponds to amino acids 1-543 of H53393_PEA_(—)1_P2 (SEQ ID NO: 185), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence GK corresponding to amino acids 544-545 of H53393_PEA_(—)1_P2 (SEQ ID NO: 185), wherein said first and second amino acid sequences are contiguous and in a sequential order.

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for H53393_PEA_(—)1_P3 (SEQ ID NO: 186), comprising a first amino acid sequence being at least 90% homologous to MRTYRYFLLLFWVGQPYPTLSTPLSKRTSGFPAKKRALELSGNSKNELNRSKRSWMWN QFFLLEEYTGSDYQYVGKLHSDQDRGDGSLKYILSGDGAGDLFIINENTGDIQATKRLD REEKPVYILRAQAINRRTGRPVEPESEFIIKIHDINDNEPIFTKEVYTATVPEMSDVGTFVV QVTATDADDPTYGNSAKVVYSILQGQPYFSVESETGIIKTALLNMDRENREQYQVVIQA KDMGGQMGGLSGTTTVNITLTDVNDNPPRFPQSTYQFKTPESSPPGTPIGRIKASDADV GENAEIEYSITDGEGLDMFDVITDQETQEGIITVKKLLDFEKKKVYTLKVEASNPYVEPR FLYLGPFKDSATVRIVVEDVDEPPVFSKLAYILQIREDAQINTTIGSVTAQDPDAARNPV KYSVDRHTDMDRIFNIDSGNGSIFTSKLLDRETLLWHNITVIATEINNPKQSSRVPLYIKV LDVNDNAPEFAEFYETFVCEKAKADQ corresponding to amino acids 1-504 of CAD6_HUMAN, which also corresponds to amino acids 1-504 of H53393_PEA_(—)1_P3 (SEQ ID NO: 186), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence RFGFSLS (SEQ ID NO: 1133) corresponding to amino acids 505-511 of H53393_PEA_(—)1_P3 (SEQ ID NO: 186), wherein said first and second amino acid sequences are contiguous and in a sequential order.

According to preferred embodiments of the present invention, there is provided an isolated polypeptide encoding for a tail of H53393_PEA_(—)1_P3 (SEQ ID NO: 186), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence RFGFSLS (SEQ ID NO: 1133) in H53393_PEA_(—)1_P3 (SEQ ID NO: 186).

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for H53393_PEA_(—)1_P6 (SEQ ID NO: 187), comprising a first amino acid sequence being at least 90% homologous to MRTYRYFLLLFWVGQPYPTLSTPLSKRTSGFPAKKRALELSGNSKNELNRSKRSWMWN QFFLLEEYTGSDYQYVGKLHSDQDRGDGSLKYILSGDGAGDLFIINENTGDIQATKRLD REEKPVYILRAQAINRRTGRPVEPESEFIIKIHDINDNEPIFTKEVYTATVPEMSDVGTFVV QVTATDADDPTYGNSAKVVYSILQGQPYFSVESETGIIKTALLNMDRENREQYQVVIQA KDMGGQMGGLSGTTTVNITLTDVNDNPPRFPQSTYQFKTPESSPPGTPIGRIKASDADV GENAEIEYSITDGEGLDMFDVITDQETQEGIITVKK corresponding to amino acids 1-333 of CAD6_HUMAN, which also corresponds to amino acids 1-333 of H53393_PEA_(—)1_P6 (SEQ ID NO: 187), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence VMPLLKHHTE (SEQ ID NO: 1134) corresponding to amino acids 334-343 of H53393_PEA_(—)1_P6 (SEQ ID NO: 187), wherein said first and second amino acid sequences are contiguous and in a sequential order.

According to preferred embodiments of the present invention, there is provided an isolated polypeptide encoding for a tail of H53393_PEA_(—)1_P6 (SEQ ID NO: 187), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence VMPLLKHHTE (SEQ ID NO: 1134) in H53393_PEA_(—)1_P6 (SEQ ID NO: 187).

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for HSU40434_PEA_(—)1_P12 (SEQ ID NO: 226), comprising a first amino acid sequence being at least 90% homologous to MALPTARPLLGSCGTPALGSLLFLLFSLGWVQPSRTLAGETGQEAAPLDGVLANPPNISS LSPRQLLGFPCAEVSGLSTERVRELAVALAQKNVKLSTEQLRCLAHRLSEPPEDLDALP LDLLLFLNPDAFSGPQACTRFFSRITKANVDLLPRGAPERQRLLPAALACWGVRGSLLS EADVRALGGLACDLPGRFVAESAEVLLPRLVSCPGPLDQDQQEAARAALQGGGPPYGP PSTWSVSTMDALRGLLPVLGQPIIRSIPQGIVAAWRQRSSRDPSWRQPERTILRPRFRRE VEKTACPSGKKAREIDESLIFYKKWELEACVDAALLATQMDRVNAIPFTYEQLDVLKH KLDELYPQGYPESVIQHLGYLFLKMSPEDIRKWNVTSLETLKALLEVNKGHEMSPQVA TLIDRFVKGRGQLDKDTLDTLTAFYPGYLCSLSPEELSSVPPSSIW corresponding to amino acids 1-458 of Q14859 (SEQ ID NO: 985), which also corresponds to amino acids 1-458 of HSU40434_PEA_(—)1_P12 (SEQ ID NO: 226).

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for HSU40434_PEA_(—)1_P12 (SEQ ID NO: 226), comprising a first amino acid sequence being at least 90% homologous to MALPTARPLLGSCGTPALGSLLFLLFSLGWVQPSRTLAGETGQ corresponding to amino acids 1-43 of Q9BTR2 (SEQ ID NO: 986), which also corresponds to amino acids 1-43 of HSU40434_PEA_(—)1_P12 (SEQ ID NO: 226), second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence E corresponding to amino acids 44-44 of HSU40434_PEA_(—)1_P12 (SEQ ID NO: 226), and a third amino acid sequence being at least 90% homologous to AAPLDGVLANPPNISSLSPRQLLGFPCAEVSGLSTERVRELAVALAQKNVKLSTEQLRC LAHRLSEPPEDLDALPLDLLLFLNPDAFSGPQACTRFFSRITKANVDLLPRGAPERQRLL PAALACWGVRGSLLSEADVRALGGLACDLPGRFVAESAEVLLPRLVSCPGPLDQDQQE AARAALQGGGPPYGPPSTWSVSTMDALRGLLPVLGQPIIRSIPQGIVAAWRQRSSRDPS WRQPERTILRPRFRREVEKTACPSGKKAREIDESLIFYKKWELEACVDAALLATQMDRV NAIPFTYEQLDVLKHKLDELYPQGYPESVIQHLGYLFLKMSPEDIRKWNVTSLETLKAL LEVNKGHEMSPQVATLIDRFVKGRGQLDKDTLDTLTAFYPGYLCSLSPEELSSVPPSSIW corresponding to amino acids 44-457 of Q9BTR2 (SEQ ID NO: 986), which also corresponds to amino acids 45-458 of HSU40434_PEA_(—)1_P12 (SEQ ID NO: 226), wherein said first, second and third amino acid sequences are contiguous and in a sequential order.

According to preferred embodiments of the present invention, there is provided an isolated polypeptide encoding for an edge portion of HSU40434_PEA_(—)1_P12 (SEQ ID NO: 226), comprising an amino acid sequence being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence encoding for E, corresponding to HSU40434_PEA_(—)1_P12 (SEQ ID NO: 226).

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for M77904_P2 (SEQ ID NO: 252), comprising a first amino acid sequence being at least 90% homologous to MLSIKSGERIVFTFSCQSPENHFVIEIQKNIDCMSGPCPFGEVQLQPSTSLLPTLNRTFIWD VKAHKSIGLELQFSIPRLRQIGPGESCPDGVTHSISGRIDATVVRIGTFCSNGTVSRIKMQ EGVKMALHLPWFHPRNVSGFSIANRSSIKRLCIIESVFEGEGSATLMSANYPEGFPEDEL MTWQFVVPAHLRASVSFLNFNLSNCERKEERVEYYIPGSTTNPEVFKLEDKQPGNMAG NFNLSLQGCDQDAQSPGILRLQFQVLVQHPQNES corresponding to amino acids 67-341 of Q8WU91 (SEQ ID NO: 987), which also corresponds to amino acids 1-275 of M77904_P2 (SEQ ID NO: 252), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence NKIYVVDLSNERAMSLTIEPRPVKQSRKFVPGCFVCLESRTCSSNLTLTSGSKHKISFLCD DLTRLWMNVEKTISCTDHRYCQRKSYSLQVPSDILHLPVELHDFSWKLLVPKDRLSLVL VPAQKLQQHTHEKPCNTSFSYLVASAIPSQDLYFGSFCPGGSIKQIQVKQNISVTLRTFAP SFQQEASRQGLTVSFIPYFKEEGVFTVTPDTKSKVYLRTPNWDRGLPSLTSVSWNISVPR DQVACLTFFKERSGVVCQTGRAFMIIQEQRTRAEEIFSLDEDVLPKPSFHHHSFWVNISN CSPTSGKQLDLLFSVTLTPRTVDLTVILIAAVGGGVLLLSALGLIICCVKKKKKKTNKGP AVGIYNGNINTEMPRQPKKFQKGRKDNDSHVYAVIEDTMVYGHLLQDSSGSFLQPEVD TYRPFQGTMGVCPPSPPTICSRAPTAKLATEEPPPRSPPESESEPYTFSHPNNGDVSSKDT DIPLLNTQEPMEPAE (SEQ ID NO: 1135) corresponding to amino acids 276-770 of M77904_P2 (SEQ ID NO: 252), wherein said first and second amino acid sequences are contiguous and in a sequential order.

According to preferred embodiments of the present invention, there is provided an isolated polypeptide encoding for a tail of M77904_P2 (SEQ ID NO: 252), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence NKIYVVDLSNERAMSLTIEPRPVKQSRKFVPGCFVCLESRTCSSNLTLTSGSKHKISFLCD DLTRLWMNVEKTISCTDHRYCQRKSYSLQVPSDILHLPVELHDFSWKLLVPKDRLSLVL VPAQKLQQHTHEKPCNTSFSYLVASAIPSQDLYFGSFCPGGSIKQIQVKQNISVTLRTFAP SFQQEASRQGLTVSFIPYFKEEGVFTVTPDTKSKVYLRTPNWDRGLPSLTSVSWNISVPR DQVACLTFFKERSGVVCQTGRAFMIIQEQRTRAEEIFSLDEDVLPKPSFHHHSFWVNISN CSPTSGKQLDLLFSVTLTPRTVDLTVILIAAVGGGVLLLSALGLIICCVKKKKKKTNKGP AVGIYNGNINTEMPRQPKKFQKGRKDNDSHVYAVIEDTMVYGHLLQDSSGSFLQPEVD TYRPFQGTMGVCPPSPPTICSRAPTAKLATEEPPPRSPPESESEPYTFSHPNNGDVSSKDT DIPLLNTQEPMEPAE (SEQ ID NO: 1135) in M77904_P2 (SEQ ID NO: 252).

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for M77904_P2 (SEQ ID NO: 252), comprising a first amino acid sequence being at least 90% homologous to MLSIKSGERIVFTFSCQSPENHFVIEIQKNIDCMSGPCPFGEVQLQPSTSLLPTLNRTFIWD VKAHKSIGLELQFSIPRLRQIGPGESCPDGVTHSISGRIDATVVRIGTFCSNGTVSRIKMQ EGVKMALHLPWFHPRNVSGFSIANRSSIKRLCIIESVFEGEGSATLMSANYPEGFPEDEL MTWQFVVPAHLRASVSFLNFNLSNCERKEERVEYYIPGSTTNPEVFKLEDKQPGNMAG NFNLSLQGCDQDAQSPGILRLQFQVLVQHPQNESNKIYVVDLSNERAMSLTIEPRPVKQ SRKFVPGCFVCLESRTCSSNLTLTSGSKHKISFLCDDLTRLWMNVEKTISCTDHRYCQR KSYSLQVPSDILHLPVELHDFSWKLLVPKDRLSLVLVPAQKLQQHTHEKPCNTSFSYLV ASAIPSQDLYFGSFCPGGSIKQIQVKQNISVTLRTFAPSFQQEASRQGLTVSFIPYFKEEGV FTVTPDTKSKVYLRTPNWDRGLPSLTSVSWNISVPRDQVACLTFFKERSGVVCQTGRAF MIIQEQRTRAEEIFSLDEDVLPKPSFHHHSFWVNISNCSPTSGKQLDLLFSVTLTPRTVDL TVILIAAVGGGVLLLSALGLIICCVKKKKKKTNKGPAVGIYNGNINTEMPRQPKKFQKG RKDNDSHVYAVIEDTMVYGHLLQDSSGSFLQPEVDTYRPFQGTMGVCPPSPPTICSRAP TAKLATEEPPPRSPPESESEPYTFSHPNNGDVSSKDTDIPLLNTQEPMEPAE (SEQ ID NO: 1135) corresponding to amino acids 67-836 of Q96QU7 (SEQ ID NO: 988), which also corresponds to amino acids 1-770 of M77904_P2 (SEQ ID NO: 252).

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for M77904_P4 (SEQ ID NO: 253), comprising a first amino acid sequence being at least 90% homologous to MAGLNCGVSIALLGVLLLGAARLPRGAEAFEIALPRESNITVLIKLGTPTLLAKPCYIVIS KRHITMLSIKSGERIVFTFSCQSPENHFVIEIQKNIDCMSGPCPFGEVQLQPSTSLLPTLNR TFIWDVKAHKSIGLELQFSIPRLRQIGPGESCPDGVTHSISGRIDATVVRIGTFCSNGTVSR IKMQEGVKMALHLPWFHPRNVSGFSIANRSSIKRLCIIESVFEGEGSATLMSANYPEGFP EDELMTWQFVVPAHLRASVSFLNFNLSNCERKEERVEYYIPGSTTNPEVFKLEDKQPGN MAGNFNLSLQGCDQDAQSPGILRLQFQVLVQHPQNES corresponding to amino acids 1-341 of Q8WU91 (SEQ ID NO: 987), which also corresponds to amino acids 1-341 of M77904_P4 (SEQ ID NO: 253), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence NKIYVVDLSNERAMSLTIEPRPVKQSRKFVPGCFVCLESRTCSSNLTLTSGSKHKISFLCD DLTRLWMNVEKTISTPLNQCICPWPWIALLSPPCLSGVPWVGCKSYQKGPSGRARWLT PVIPALWEAKAGGSLEVRSSRPAWPTW (SEQ ID NO: 1136) corresponding to amino acids 342-487 of M77904_P4 (SEQ ID NO: 253), wherein said first and second amino acid sequences are contiguous and in a sequential order.

According to preferred embodiments of the present invention, there is provided an isolated polypeptide encoding for a tail of M77904_P4 (SEQ ID NO: 253), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence NKIYVVDLSNERAMSLTIEPRPVKQSRKFVPGCFVCLESRTCSSNLTLTSGSKHKISFLCD DLTRLWMNVEKTISTPLNQCICPWPWIALLSPPCLSGVPWVGCKSYQKGPSGRARWLT PVIPALWEAKAGGSLEVRSSRPAWPTW (SEQ ID NO: 1136) in M77904_P4 (SEQ ID NO: 253).

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for M77904_P4 (SEQ ID NO: 253), comprising a first amino acid sequence being at least 90% homologous to MAGLNCGVSIALLGVLLLGAARLPRGAEAFEIALPRESNITVLIKLGTPTLLAKPCYIVIS KRHITMLSIKSGERIVFTFSCQSPENHFVIEIQKNIDCMSGPCPFGEVQLQPSTSLLPTLNR TFIWDVKAHKSIGLELQFSIPRLRQIGPGESCPDGVTHSISGRIDATVVRIGTFCSNGTVSR IKMQEGVKMALHLPWFHPRNVSGFSIANRSSIKRLCIIESVFEGEGSATLMSANYPEGFP EDELMTWQFVVPAHLRASVSFLNFNLSNCERKEERVEYYIPGSTTNPEVFKLEDKQPGN MAGNFNLSLQGCDQDAQSPGILRLQFQVLVQHPQNESNKIYVVDLSNERAMSLTIEPRP VKQSRKFVPGCFVCLESRTCSSNLTLTSGSKHKISFLCDDLTRLWMNVEKTIS corresponding to amino acids 1-416 of Q9H5V8 (SEQ ID NO: 989), which also corresponds to amino acids 1-416 of M77904_P4 (SEQ ID NO: 253), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence TPLNQCICPWPWIALLSPPCLSGVPWVGCKSYQKGPSGRARWLTPVIPALWEAKAGGS LEVRSSRPAWPTW corresponding to amino acids 417-487 of M77904_P4 (SEQ ID NO: 253), wherein said first and second amino acid sequences are contiguous and in a sequential order.

According to preferred embodiments of the present invention, there is provided an isolated polypeptide encoding for a tail of M77904_P4 (SEQ ID NO: 253), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence TPLNQCICPWPWIALLSPPCLSGVPWVGCKSYQKGPSGRARWLTPVIPALWEAKAGGS LEVRSSRPAWPTW in M77904_P4 (SEQ ID NO: 253).

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for M77904_P4 (SEQ ID NO: 253), comprising a first amino acid sequence being at least 90% homologous to MAGLNCGVSIALLGVLLLGAARLPRGAEAFEIALPRESNITVLIKLGTPTLLAKPCYIVIS KRHITMLSIKSGERIVFTFSCQSPENHFVIEIQKNIDCMSGPCPFGEVQLQPSTSLLPTLNR TFIWDVKAHKSIGLELQFSIPRLRQIGPGESCPDGVTHSISGRIDATVVRIGTFCSNGTVSR IKMQEGVKMALHLPWFHPRNVSGFSIANRSSIKRLCIIESVFEGEGSATLMSANYPEGFP EDELMTWQFVVPAHLRASVSFLNFNLSNCERKEERVEYYIPGSTTNPEVFKLEDKQPGN MAGNFNLSLQGCDQDAQSPGILRLQFQVLVQHPQNESNKIYVVDLSNERAMSLTIEPRP VKQSRKFVPGCFVCLESRTCSSNLTLTSGSKHKISFLCDDLTRLWMNVEKTIS corresponding to amino acids 1-416 of Q96QU7 (SEQ ID NO: 988), which also corresponds to amino acids 1-416 of M77904_P4 (SEQ ID NO: 253), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence TPLNQCICPWPWIALLSPPCLSGVPWVGCKSYQKGPSGRARWLTPVIPALWEAKAGGS LEVRSSRPAWPTW corresponding to amino acids 417-487 of M77904_P4 (SEQ ID NO: 253), wherein said first and second amino acid sequences are contiguous and in a sequential order.

According to preferred embodiments of the present invention, there is provided an isolated polypeptide encoding for a tail of M77904_P4 (SEQ ID NO: 253), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence TPLNQCICPWPWIALLSPPCLSGVPWVGCKSYQKGPSGRARWLTPVIPALWEAKAGGS LEVRSSRPAWPTW in M77904_P4 (SEQ ID NO: 253).

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for M77904_P5 (SEQ ID NO: 254), comprising a first amino acid sequence being at least 90% homologous to MIIQEQRTRAEEIFSLDEDVLPKPSFHHHSFWVNISNCSPTSGKQLDLLFSVTLTPRTVDL TVILIAAVGGGVLLLSALGLIICCVKKKKKKTNKGPAVGIYNGNINTEMPRQPKKFQKG RKDNDSHVYAVIEDTMVYGHLLQDSSGSFLQPEVDTYRPFQGTMGVCPPSPPTICSRAP TAKLATEEPPPRSPPESESEPYTFSHPNNGDVSSKDTDIPLLNTQEPMEPAE corresponding to amino acids 606-836 of Q96QU7 (SEQ ID NO: 988), which also corresponds to amino acids 1-231 of M77904_P5 (SEQ ID NO: 254).

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for M77904_P5 (SEQ ID NO: 254), comprising a first amino acid sequence being at least 90% homologous to MIIQEQRTRAEEIFSLDEDVLPKPSFHHHSFWVNISNCSPTSGKQLDLLFSVTLTPRTVDL TVILIAAVGGGVLLLSALGLIICCVKKKKKKTNKGPAVGIYNGNINTEMPRQPKKFQKG RKDNDSHVYAVIEDTMVYGHLLQDSSGSFLQPEVDTYRPFQGTMGVCPPSPPTICSRAP TAKLATEEPPPRSPPESESEPYTFSHPNNGDVSSKDTDIPLLNTQEPMEPAE corresponding to amino acids 419-649 of Q9H₈C2 (SEQ ID NO: 990), which also corresponds to amino acids 1-231 of M77904_P5 (SEQ ID NO: 254).

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for M77904_P7 (SEQ ID NO: 255), comprising a first amino acid sequence being at least 90% homologous to MAGLNCGVSIALLGVLLLGAARLPRGAEAFEIALPRESNITVLIKLGTPTLLAKPCYIVIS KRHITMLSIKSGERIVFTFSCQSPENHFVIEIQKNIDCMSGPCPFGEVQLQPSTSLLPTLNR TFIWDVKAHKSIGLELQFSIPRLRQIGPGESCPDGVTHSISGRIDATVVRIGTFCSNGTVSR IKMQEGVKMALHLPWFHPRNVSGFSIANRSSIKR corresponding to amino acids 1-219 of Q8WU91 (SEQ ID NO: 987), which also corresponds to amino acids 1-219 of M77904_P7 (SEQ ID NO: 255), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence EKAPPCYLIRLKHTRSSLF (SEQ ID NO: 1137) corresponding to amino acids 220-238 of M77904_P7 (SEQ ID NO: 255), wherein said first and second amino acid sequences are contiguous and in a sequential order.

According to preferred embodiments of the present invention, there is provided an isolated polypeptide encoding for a tail of M77904_P7 (SEQ ID NO: 255), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence EKAPPCYLIRLKHTRSSLF (SEQ ID NO: 1137) in M77904_P7 (SEQ ID NO: 255).

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for M77904_P7 (SEQ ID NO: 255), comprising a first amino acid sequence being at least 90% homologous to MAGLNCGVSIALLGVLLLGAARLPRGAEAFEIALPRESNITVLIKLGTPTLLAKPCYIVIS KRHITMLSIKSGERIVFTFSCQSPENHFVIEIQKNIDCMSGPCPFGEVQLQPSTSLLPTLNR TFIWDVKAHKSIGLELQFSIPRLRQIGPGESCPDGVTHSISGRIDATVVRIGTFCSNGTVSR IKMQEGVKMALHLPWFHPRNVSGFSIANRSSIKR corresponding to amino acids 1-219 of Q9H5V8 (SEQ ID NO: 989), which also corresponds to amino acids 1-219 of M77904_P7 (SEQ ID NO: 255), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence EKAPPCYLIRLKHTRSSLF (SEQ ID NO: 1137) corresponding to amino acids 220-238 of M77904_P7 (SEQ ID NO: 255), wherein said first and second amino acid sequences are contiguous and in a sequential order.

According to preferred embodiments of the present invention, there is provided an isolated polypeptide encoding for a tail of M77904_P7 (SEQ ID NO: 255), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence EKAPPCYLIRLKHTRSSLF (SEQ ID NO: 1137) in M77904_P7 (SEQ ID NO: 255).

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for M77904_P7 (SEQ ID NO: 255), comprising a first amino acid sequence being at least 90% homologous to MAGLNCGVSIALLGVLLLGAARLPRGAEAFEIALPRESNITVLIKLGTPTLLAKPCYIVIS KRHITMLSIKSGERIVFTFSCQSPENHFVIEIQKNIDCMSGPCPFGEVQLQPSTSLLPTLNR TFIWDVKAHKSIGLELQFSIPRLRQIGPGESCPDGVTHSISGRIDATVVRIGTFCSNGTVSR IKMQEGVKMALHLPWFHPRNVSGFSIANRSSIKR corresponding to amino acids 1-219 of Q96QU7 (SEQ ID NO: 988), which also corresponds to amino acids 1-219 of M77904_P7 (SEQ ID NO: 255), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence EKAPPCYLIRLKHTRSSLF (SEQ ID NO: 1137) corresponding to amino acids 220-238 of M77904_P7 (SEQ ID NO: 255), wherein said first and second amino acid sequences are contiguous and in a sequential order.

According to preferred embodiments of the present invention, there is provided an isolated polypeptide encoding for a tail of M77904_P7 (SEQ ID NO: 255), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence EKAPPCYLIRLKHTRSSLF (SEQ ID NO: 1137) in M77904_P7 (SEQ ID NO: 255).

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for Z25299_PEA_(—)2_P2 (SEQ ID NO: 273), comprising a first amino acid sequence being at least 90% homologous to MKSSGLFPFLVLLALGTLAPWAVEGSGKSFKAGVCPPKKSAQCLRYKKPECQSDWQCP GKKRCCPDTCGIKCLDPVDTPNPTRRKPGKCPVTYGQCLMLNPPNFCEMDGQCKRDLK CCMGMCGKSCVSPVK corresponding to amino acids 1-131 of ALK1_HUMAN, which also corresponds to amino acids 1-131 of Z25299_PEA_(—)2_P2 (SEQ ID NO: 273), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence GKQGMRAH (SEQ ID NO: 1138) corresponding to amino acids 132-139 of Z25299_PEA_(—)2_P2 (SEQ ID NO: 273), wherein said first and second amino acid sequences are contiguous and in a sequential order.

According to preferred embodiments of the present invention, there is provided an isolated polypeptide encoding for a tail of Z25299_PEA_(—)2_P2 (SEQ ID NO: 273), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence GKQGMRAH (SEQ ID NO: 1138) in Z25299_PEA_(—)2_P2 (SEQ ID NO: 273).

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for Z25299_PEA_(—)2_P3 (SEQ ID NO: 274), comprising a first amino acid sequence being at least 90% homologous to MKSSGLFPFLVLLALGTLAPWAVEGSGKSFKAGVCPPKKSAQCLRYKKPECQSDWQCP GKKRCCPDTCGIKCLDPVDTPNPTRRKPGKCPVTYGQCLMLNPPNFCEMDGQCKRDLK CCMGMCGKSCVSPVK corresponding to amino acids 1-131 of ALK1_HUMAN, which also corresponds to amino acids 1-131 of Z25299_PEA_(—)2_P3 (SEQ ID NO: 274), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence GEKRHHKQLRDQEVDPLEMRRHSAG (SEQ ID NO: 1139) corresponding to amino acids 132-156 of Z25299_PEA_(—)2_P3 (SEQ ID NO: 274), wherein said first and second amino acid sequences are contiguous and in a sequential order.

According to preferred embodiments of the present invention, there is provided an isolated polypeptide encoding for a tail of Z25299_PEA_(—)2_P3 (SEQ ID NO: 274), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence GEKRHHKQLRDQEVDPLEMRRHSAG (SEQ ID NO: 1139) in Z25299_PEA_(—)2_P3 (SEQ ID NO: 274).

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for Z25299_PEA_(—)2_P7 (SEQ ID NO: 275), comprising a first amino acid sequence being at least 90% homologous to MKSSGLFPFLVLLALGTLAPWAVEGSGKSFKAGVCPPKKSAQCLRYKKPECQSDWQCP GKKRCCPDTCGIKCLDPVDTPNP corresponding to amino acids 1-81 of ALK1_HUMAN, which also corresponds to amino acids 1-81 of Z25299_PEA_(—)2_P7 (SEQ ID NO: 275), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence RGSLGSAQ (SEQ ID NO: 1140) corresponding to amino acids 82-89 of Z25299_PEA_(—)2_P7 (SEQ ID NO: 275), wherein said first and second amino acid sequences are contiguous and in a sequential order.

According to preferred embodiments of the present invention, there is provided an isolated polypeptide encoding for a tail of Z25299_PEA_(—)2_P7 (SEQ ID NO: 275), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence RGSLGSAQ (SEQ ID NO: 1140) in Z25299_PEA_(—)2_P7 (SEQ ID NO: 275).

According to preferred embodiments of the present invention, there is provided an isolated chimeric polypeptide encoding for Z25299_PEA_(—)2_P10 (SEQ ID NO: 276), comprising a first amino acid sequence being at least 90% homologous to MKSSGLFPFLVLLALGTLAPWAVEGSGKSFKAGVCPPKKSAQCLRYKKPECQSDWQCP GKKRCCPDTCGIKCLDPVDTPNPT corresponding to amino acids 1-82 of ALK1_HUMAN, which also corresponds to amino acids 1-82 of Z25299_PEA_(—)2_P10 (SEQ ID NO: 276).

According to preferred embodiments of the present invention, there is provided an antibody capable of specifically binding to an epitope of an amino acid sequence as described herein.

Optionally the amino acid sequence corresponds to a bridge, edge portion, tail, head or insertion as described herein.

Optionally the antibody is capable of differentiating between a splice variant having said epitope and a corresponding known protein.

According to preferred embodiments of the present invention, there is provided a kit for detecting ovarian cancer, comprising a kit detecting overexpression of a splice variant as described herein.

Optionally the kit comprises a NAT-based technology.

Optionally the kit further comprises at least one primer pair capable of selectively hybridizing to a nucleic acid sequence as described herein.

Optionally the kit further comprises at least one oligonucleotide capable of selectively hybridizing to a nucleic acid sequence as described herein.

Optionally the kit comprises an antibody as described herein.

Optionally the kit further comprises at least one reagent for performing an ELISA or a Western blot.

According to preferred embodiments of the present invention, there is provided a method for detecting ovarian cancer, comprising detecting overexpression of a splice variant as described herein.

Optionally detecting overexpression is performed with a NAT-based technology.

Optionally detecting overexpression is performed with an immunoassay.

Optionally the immunoassay comprises an antibody as described herein.

According to preferred embodiments of the present invention, there is provided a biomarker capable of detecting ovarian cancer, comprising any of the above nucleic acid sequences or a fragment thereof, or any of the above amino acid sequences or a fragment thereof.

According to preferred embodiments of the present invention, there is provided a method for screening for ovarian cancer, comprising detecting ovarian cancer cells with a biomarker or an antibody or a method or assay as described herein.

According to preferred embodiments of the present invention, there is provided a method for diagnosing ovarian cancer, comprising detecting ovarian cancer cells with a biomarker or an antibody or a method or assay as described herein.

According to preferred embodiments of the present invention, there is provided a method for monitoring disease progression and/or treatment efficacy and/or relapse of ovarian cancer, comprising detecting ovarian cancer cells with a biomarker or an antibody or a method or assay as described herein.

According to preferred embodiments of the present invention, there is provided a method of selecting a therapy for ovarian cancer, comprising detecting ovarian cancer cells with a biomarker or an antibody or a method or assay as described herein and selecting a therapy according to said detection.

According to preferred embodiments of the present invention, preferably any of the above nucleic acid and/or amino acid sequences further comprises any sequence having at least about 70%, preferably at least about 80%, more preferably at least about 90%, most preferably at least about 95% homology thereto.

Unless otherwise noted, all experimental data relates to variants of the present invention, named according to the segment being tested (as expression was tested through RT-PCR as described).

All nucleic acid sequences and/or amino acid sequences shown herein as embodiments of the present invention relate to their isolated form, as isolated polynucleotides (including for all transcripts), oligonucleotides (including for all segments, amplicons and primers), peptides (including for all tails, bridges, insertions or heads, optionally including other antibody epitopes as described herein) and/or polypeptides (including for all proteins). It should be noted that oligonucleotide and polynucleotide, or peptide and polypeptide, may optionally be used interchangeably.

BRIEF DESCRIPTION OF DRAWINGS

FIG. 1 is schematic summary of cancer biomarkers selection engine and the wet validation stages.

FIG. 2. Schematic illustration, depicting grouping of transcripts of a given cluster based on presence or absence of unique sequence regions.

FIG. 3 is schematic summary of quantitative real-time PCR analysis.

FIG. 4 is schematic presentation of the oligonucleotide based microarray fabrication.

FIG. 5 is schematic summary of the oligonucleotide based microarray experimental flow.

FIG. 6 shows cancer and cell-line vs. normal tissue expression for.

FIG. 7 shows expression of segment8 in H61775 in cancerous vs. non-cancerous tissues.

FIG. 8 shows expression of segment8 in H61775 in normal tissues.

FIG. 9 shows cancer and cell-line vs. normal tissue expression.

FIG. 10 is a histogram showing over expression of T10888junc11-17 (SEQ ID NO:962) transcripts in cancerous ovary samples relative to the normal samples.

FIG. 11 is a histogram showing expression of T10888junc11-17 (SEQ ID NO:962) transcripts in normal tissues.

FIG. 12 shows cancer and cell-line vs. normal tissue expression.

FIG. 13 is a histogram showing over expression of HUMGRP5Ejunc3-7 (SEQ ID NO:967) transcripts in cancerous ovary samples relative to the normal samples.

FIG. 14 is a histogram showing expression of HUMGRP5Ejunc3-7 (SEQ ID NO:967) transcripts in normal tissues.

FIG. 15 shows cancer and cell-line vs. normal tissue expression.

FIG. 16 is a histogram showing over expression of R11723 seg13 (SEQ ID NO:975) transcripts in cancerous ovary samples relative to the normal PM samples.

FIG. 17 is a histogram showing expression of R11723 seg13 (SEQ ID NO:975) transcripts in normal tissue samples.

FIG. 18 is a histogram showing over expression of R11723 junc11-18 (SEQ ID NO:978) transcripts in cancerous ovary samples relative to the normal samples.

FIG. 19 is a histogram showing expression of R11723 junc11-18 (SEQ ID NO:978) transcripts in normal tissue samples.

FIG. 20 shows cancer and cell-line vs. normal tissue expression.

FIG. 21 is a histogram showing over expression of H53393 seg13 (SEQ ID NO:981) transcripts in cancerous ovary samples relative to the normal samples.

FIG. 22 is a histogram showing over expression of H53393 junc21-22 (SEQ ID NO:984) transcripts in cancerous ovary samples relative to the normal samples.

FIG. 23 shows cancer and cell-line vs. normal tissue expression.

FIG. 24 shows cancer and cell-line vs. normal tissue expression.

FIG. 25 shows cancer and cell-line vs. normal tissue expression.

FIG. 26 is a histogram showing over expression of Z25299 junc13-14-21 (SEQ ID NO:993) transcripts in cancerous ovary samples relative to the normal samples.

FIGS. 27A and 27B are histograms showing over expression of Z25299 seg20 (SEQ ID NO:996) transcripts in cancerous ovary samples relative to the normal samples (27A) or in normal tissues (27B).

FIGS. 28A and 28B are histograms showing over expression of Z25299 seg23 (SEQ ID NO:999) transcripts in cancerous ovary samples relative to the normal samples (28A) or in normal tissues (28B).

FIG. 29 shows cancer and cell-line vs. normal tissue expression.

FIG. 30 is a histogram showing down regulation of T39971 junc23-33R (SEQ ID NO: 1003) transcripts in cancerous ovary samples relative to the normal samples.

FIG. 31 is a histogram showing expression of T39971 junc23-33R (SEQ ID NO:1003) transcripts in normal tissues.

FIG. 32 shows cancer and cell-line vs. normal tissue expression.

FIGS. 33A and 33B are histograms showing down regulation of Z44808 junc8-11 (SEQ ID NO: 1006) transcripts in cancerous ovary samples relative to the normal samples (33A) or expression in normal tissues (33B).

FIG. 34 shows cancer and cell-line vs. normal tissue expression.

FIG. 35 shows cancer and cell-line vs. normal tissue expression.

FIG. 36 shows cancer and cell-line vs. normal tissue expression.

FIG. 37 shows cancer and cell-line vs. normal tissue expression.

FIG. 38 shows cancer and cell-line vs. normal tissue expression.

FIG. 39 shows cancer and cell-line vs. normal tissue expression.

FIG. 40 shows cancer and cell-line vs. normal tissue expression.

FIG. 41 shows cancer and cell-line vs. normal tissue expression.

FIG. 42 shows cancer and cell-line vs. normal tissue expression.

FIG. 43 is a histogram showing differential expression of a variety of transcripts in cancerous ovary samples relative to the normal samples.

FIG. 44 shows cancer and cell-line vs. normal tissue expression.

DESCRIPTION OF PREFERRED EMBODIMENTS

The present invention is of novel markers for ovarian cancer that are both sensitive and accurate. Biomolecular sequences (amino acid and/or nucleic acid sequences) uncovered using the methodology of the present invention and described herein can be efficiently utilized as tissue or pathological markers and/or as drugs or drug targets for treating or preventing a disease.

Furthermore, at least certain of these markers are able to distinguish between various types of ovarian cancer, such as Ovarian epithelial tumors (serous, mucinous, endometroid, clear cell, and Brenner tumor), ovarian germ-cell tumors, (teratoma, dysgerminoma, endodermal sinus tumor, and embryonal carcinoma) and ovarian stromal tumors (originating from granulosa, theca, Sertoli, Leydig, and collagen-producing stromal cells), alone or in combination. These markers are differentially expressed, and preferably overexpressed in ovarian cancer specifically, as opposed to normal ovarian tissue. The measurement of these markers, alone or in combination, in patient samples provides information that the diagnostician can correlate with a probable diagnosis of ovarian cancer. The markers of the present invention, alone or in combination, show a high degree of differential detection between ovarian cancer and non-cancerous states.

The markers of the present invention, alone or in combination, can be used for prognosis, prediction, screening, early diagnosis, staging, therapy selection and treatment monitoring of ovarian cancer. For example, optionally and preferably, these markers may be used for staging ovarian cancer and/or monitoring the progression of the disease. Furthermore, the markers of the present invention, alone or in combination, can be used for detection of the source of metastasis found in anatomical places other thenovary. Also, one or more of the markers may optionally be used in combination with one or more other ovarian cancer markers (other than those described herein). According to an optional embodiment of the present invention, such a combination may be used to differentiate between various types of ovarian cancer, such as Ovarian epithelial tumors (serous, mucinous, endometroid, clear cell, and Brenner tumor), ovarian germ-cell tumors, (teratoma, dysgerminoma, endodermal sinus tumor, and embryonal carcinoma) and ovarian stromal tumors (originating from either granulosa, theca, Sertoli, Leydig, and collagen-producing stromal cells).

These markers are specifically released to the bloodstream under conditions of ovarian cancer (or one of the above indicative conditions), and/or are otherwise expressed at a much higher level and/or specifically expressed in ovarian cancer tissue or cells, and/or tissue or cells under one of the above indicative conditions. The measurement of these markers, alone or in combination, in patient samples provides information that the diagnostician can correlate with a probable diagnosis of ovarian cancer and/or a condition that it is indicative of a higher risk for ovarian cancer.

The present invention therefore also relates to diagnostic assays for ovarian cancer, and methods of use of such markers for detection of ovarian cancer, optionally and preferably in a sample taken from a subject (patient), which is more preferably some type of blood sample.

In another embodiment, the present invention relates to bridges, tails, heads and/or insertions, and/or analogs, homologs and derivatives of such peptides. Such bridges, tails, heads and/or insertions are described in greater detail below with regard to the Examples.

As used herein a “tail” refers to a peptide sequence at the end of an amino acid sequence that is unique to a splice variant according to the present invention. Therefore, a splice variant having such a tail may optionally be considered as a chimera, in that at least a first portion of the splice variant is typically highly homologous (often 100% identical) to a portion of the corresponding known protein, while at least a second portion of the variant comprises the tail.

As used herein a “head” refers to a peptide sequence at the beginning of an amino acid sequence that is unique to a splice variant according to the present invention. Therefore, a splice variant having such a head may optionally be considered as a chimera, in that at least a first portion of the splice variant comprises the head, while at least a second portion is typically highly homologous (often 100% identical) to a portion of the corresponding known protein.

As used herein “an edge portion” refers to a connection between two portions of a splice variant according to the present invention that were not joined in the wild type or known protein. An edge may optionally arise due to a join between the above “known protein” portion of a variant and the tail, for example, and/or may occur if an internal portion of the wild type sequence is no longer present, such that two portions of the sequence are now contiguous in the splice variant that were not contiguous in the known protein. A “bridge” may optionally be an edge portion as described above, but may also include a join between a head and a “known protein” portion of a variant, or a join between a tail and a “known protein” portion of a variant, or a join between an insertion and a “known protein” portion of a variant.

Optionally and preferably, a bridge between a tail or a head or a unique insertion, and a “known protein” portion of a variant, comprises at least about 10 amino acids, more preferably at least about 20 amino acids, most preferably at least about 30 amino acids, and even more preferably at least about 40 amino acids, in which at least one amino acid is from the tail/head/insertion and at least one amino acid is from the “known protein” portion of a variant. Also optionally, the bridge may comprise any number of amino acids from about 10 to about 40 amino acids (for example, 10, 11, 12, 13 . . . 37, 38, 39, 40 amino acids in length, or any number in between).

It should be noted that a bridge cannot be extended beyond the length of the sequence in either direction, and it should be assumed that every bridge description is to be read in such manner that the bridge length does not extend beyond the sequence itself.

Furthermore, bridges are described with regard to a sliding window in certain contexts below. For example, certain descriptions of the bridges feature the following format: a bridge between two edges (in which a portion of the known protein is not present in the variant) may optionally be described as follows: a bridge portion of CONTIG-NAME_P1 (representing the name of the protein), comprising a polypeptide having a length “n”, wherein n is at least about 10 amino acids in length, optionally at least about 20 amino acids in length, preferably at least about 30 amino acids in length, more preferably at least about 40 amino acids in length and most preferably at least about 50 amino acids in length, wherein at least two amino acids comprise XX (2 amino acids in the center of the bridge, one from each end of the edge), having a structure as follows (numbering according to the sequence of CONTIG-NAME_P1): a sequence starting from any of amino acid numbers 49-x to 49 (for example); and ending at any of amino acid numbers 50+((n−2)−x) (for example), in which x varies from 0 to n−2. In this example, it should also be read as including bridges in which n is any number of amino acids between 10-50 amino acids in length. Furthermore, the bridge polypeptide cannot extend beyond the sequence, so it should be read such that 49-x (for example) is not less than 1, nor 50+((n−2)−x) (for example) greater than the total sequence length.

In another embodiment, this invention provides antibodies specifically recognizing the splice variants and polypeptide fragments thereof of this invention. Preferably such antibodies differentially recognize splice variants of the present invention but do not recognize a corresponding known protein (such known proteins are discussed with regard to their splice variants in the Examples below).

In another embodiment, this invention provides an isolated nucleic acid molecule encoding for a splice variant according to the present invention, having a nucleotide sequence as set forth in any one of the sequences listed herein, or a sequence complementary thereto. In another embodiment, this invention provides an isolated nucleic acid molecule, having a nucleotide sequence as set forth in any one of the sequences listed herein, or a sequence complementary thereto. In another embodiment, this invention provides an oligonucleotide of at least about 12 nucleotides, specifically hybridizable with the nucleic acid molecules of this invention. In another embodiment, this invention provides vectors, cells, liposomes and compositions comprising the isolated nucleic acids of this invention.

In another embodiment, this invention provides a method for detecting a splice variant according to the present invention in a biological sample, comprising: contacting a biological sample with an antibody specifically recognizing a splice variant according to the present invention under conditions whereby the antibody specifically interacts with the splice variant in the biological sample but do not recognize known corresponding proteins (wherein the known protein is discussed with regard to its splice variant(s) in the Examples below), and detecting said interaction; wherein the presence of an interaction correlates with the presence of a splice variant in the biological sample.

In another embodiment, this invention provides a method for detecting a splice variant nucleic acid sequences in a biological sample, comprising: hybridizing the isolated nucleic acid molecules or oligonucleotide fragments of at least about a minimum length to a nucleic acid material of a biological sample and detecting a hybridization complex; wherein the presence of a hybridization complex correlates with the presence of a splice variant nucleic acid sequence in the biological sample.

According to the present invention, the splice variants described herein are non-limiting examples of markers for diagnosing ovarian cancer. Each splice variant marker of the present invention can be used alone or in combination, for various uses, including but not limited to, prognosis, prediction, screening, early diagnosis, determination of progression, therapy selection and treatment monitoring of ovarian cancer.

According to optional but preferred embodiments of the present invention, any marker according to the present invention may optionally be used alone or combination. Such a combination may optionally comprise a plurality of markers described herein, optionally including any subcombination of markers, and/or a combination featuring at least one other marker, for example a known marker. Furthermore, such a combination may optionally and preferably be used as described above with regard to determining a ratio between a quantitative or semi-quantitative measurement of any marker described herein to any other marker described herein, and/or any other known marker, and/or any other marker. With regard to such a ratio between any marker described herein (or a combination thereof) and a known marker, more preferably the known marker comprises the “known protein” as described in greater detail below with regard to each cluster or gene.

According to other preferred embodiments of the present invention, a splice variant protein or a fragment thereof, or a splice variant nucleic acid sequence or a fragment thereof, may be featured as a biomarker for detecting ovarian cancer and/or an indicative condition, such that a biomarker may optionally comprise any of the above.

According to still other preferred embodiments, the present invention optionally and preferably encompasses any amino acid sequence or fragment thereof encoded by a nucleic acid sequence corresponding to a splice variant protein as described herein. Any oligopeptide or peptide relating to such an amino acid sequence or fragment thereof may optionally also (additionally or alternatively) be used as a biomarker, including but not limited to the unique amino acid sequences of these proteins that are depicted as tails, heads, insertions, edges or bridges. The present invention also optionally encompasses antibodies capable of recognizing, and/or being elicited by, such oligopeptides or peptides.

The present invention also optionally and preferably encompasses any nucleic acid sequence or fragment thereof, or amino acid sequence or fragment thereof, corresponding to a splice variant of the present invention as described above, optionally for any application.

Non-limiting examples of methods or assays are described below.

The present invention also relates to kits based upon such diagnostic methods or assays.

Nucleic Acid Sequences and Oligonucleotides

Various embodiments of the present invention encompass nucleic acid sequences described hereinabove; fragments thereof, sequences hybridizable therewith, sequences homologous thereto, sequences encoding similar polypeptides with different codon usage, altered sequences characterized by mutations, such as deletion, insertion or substitution of one or more nucleotides, either naturally occurring or artificially induced, either randomly or in a targeted fashion.

The present invention encompasses nucleic acid sequences described herein; fragments thereof, sequences hybridizable therewith, sequences homologous thereto [e.g., at least 50%, at least 55%, at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 95% or more say 100% identical to the nucleic acid sequences set forth below], sequences encoding similar polypeptides with different codon usage, altered sequences characterized by mutations, such as deletion, insertion or substitution of one or more nucleotides, either naturally occurring or man induced, either randomly or in a targeted fashion. The present invention also encompasses homologous nucleic acid sequences (i.e., which form a part of a polynucleotide sequence of the present invention) which include sequence regions unique to the polynucleotides of the present invention.

In cases where the polynucleotide sequences of the present invention encode previously unidentified polypeptides, the present invention also encompasses novel polypeptides or portions thereof, which are encoded by the isolated polynucleotide and respective nucleic acid fragments thereof described hereinabove.

A “nucleic acid fragment” or an “oligonucleotide” or a “polynucleotide” are used herein interchangeably to refer to a polymer of nucleic acids. A polynucleotide sequence of the present invention refers to a single or double stranded nucleic acid sequences which is isolated and provided in the form of an RNA sequence, a complementary polynucleotide sequence (cDNA), a genomic polynucleotide sequence and/or a composite polynucleotide sequences (e.g., a combination of the above).

As used herein the phrase “complementary polynucleotide sequence” refers to a sequence, which results from reverse transcription of messenger RNA using a reverse transcriptase or any other RNA dependent DNA polymerase. Such a sequence can be subsequently amplified in vivo or in vitro using a DNA dependent DNA polymerase.

As used herein the phrase “genomic polynucleotide sequence” refers to a sequence derived (isolated) from a chromosome and thus it represents a contiguous portion of a chromosome.

As used herein the phrase “composite polynucleotide sequence” refers to a sequence, which is composed of genomic and cDNA sequences. A composite sequence can include some exonal sequences required to encode the polypeptide of the present invention, as well as some intronic sequences interposing therebetween. The intronic sequences can be of any source, including of other genes, and typically will include conserved splicing signal sequences. Such intronic sequences may further include cis acting expression regulatory elements.

Preferred embodiments of the present invention encompass oligonucleotide probes.

An example of an oligonucleotide probe which can be utilized by the present invention is a single stranded polynucleotide which includes a sequence complementary to the unique sequence region of any variant according to the present invention, including but not limited to a nucleotide sequence coding for an amino sequence of a bridge, tail, head and/or insertion according to the present invention, and/or the equivalent portions of any nucleotide sequence given herein (including but not limited to a nucleotide sequence of a node, segment or amplicon described herein).

Alternatively, an oligonucleotide probe of the present invention can be designed to hybridize with a nucleic acid sequence encompassed by any of the above nucleic acid sequences, particularly the portions specified above, including but not limited to a nucleotide sequence coding for an amino sequence of a bridge, tail, head and/or insertion according to the present invention, and/or the equivalent portions of any nucleotide sequence given herein (including but not limited to a nucleotide sequence of a node, segment or amplicon described herein).

Oligonucleotides designed according to the teachings of the present invention can be generated according to any oligonucleotide synthesis method known in the art such as enzymatic synthesis or solid phase synthesis. Equipment and reagents for executing solid-phase synthesis are commercially available from, for example, Applied Biosystems. Any other means for such synthesis may also be employed; the actual synthesis of the oligonucleotides is well within the capabilities of one skilled in the art and can be accomplished via established methodologies as detailed in, for example, “Molecular Cloning: A laboratory Manual” Sambrook et al., (1989); “Current Protocols in Molecular Biology” Volumes I-III Ausubel, R. M., ed. (1994); Ausubel et al., “Current Protocols in Molecular Biology”, John Wiley and Sons, Baltimore, Md. (1989); Perbal, “A Practical Guide to Molecular Cloning”, John Wiley & Sons, New York (1988) and “Oligonucleotide Synthesis” Gait, M. J., ed. (1984) utilizing solid phase chemistry, e.g. cyanoethyl phosphoramidite followed by deprotection, desalting and purification by for example, an automated trityl-on method or HPLC.

Oligonucleotides used according to this aspect of the present invention are those having a length selected from a range of about 10 to about 200 bases preferably about 15 to about 150 bases, more preferably about 20 to about 100 bases, most preferably about 20 to about 50 bases. Preferably, the oligonucleotide of the present invention features at least 17, at least 18, at least 19, at least 20, at least 22, at least 25, at least 30 or at least 40, bases specifically hybridizable with the biomarkers of the present invention.

The oligonucleotides of the present invention may comprise heterocylic nucleosides consisting of purines and the pyrimidines bases, bonded in a 3′ to 5′ phosphodiester linkage.

Preferably used oligonucleotides are those modified at one or more of the backbone, internucleoside linkages or bases, as is broadly described hereinunder.

Specific examples of preferred oligonucleotides useful according to this aspect of the present invention include oligonucleotides containing modified backbones or non-natural internucleoside linkages. Oligonucleotides having modified backbones include those that retain a phosphorus atom in the backbone, as disclosed in U.S. Pat. Nos. 4,469,863; 4,476,301; 5,023,243; 5,177,196; 5,188,897; 5,264,423; 5,276,019; 5,278,302; 5,286,717; 5,321,131; 5,399,676; 5,405,939; 5,453,496; 5,455,233; 5,466,677; 5,476,925; 5,519,126; 5,536,821; 5,541,306; 5,550,111; 5,563,253; 5,571,799; 5,587,361; and 5,625,050.

Preferred modified oligonucleotide backbones include, for example, phosphorothioates, chiral phosphorothioates, phosphorodithioates, phosphotriesters, aminoalkyl phosphotriesters, methyl and other alkyl phosphonates including 3′-alkylene phosphonates and chiral phosphonates, phosphinates, phosphoramidates including 3′-amino phosphoramidate and aminoalkylphosphoramidates, thionophosphoramidates, thionoalkylphosphonates, thionoalkylphosphotriesters, and boranophosphates having normal 3′-5′ linkages, 2′-5′ linked analogs of these, and those having inverted polarity wherein the adjacent pairs of nucleoside units are linked 3′-5′ to 5′-3′ or 2′-5′ to 5′-2′. Various salts, mixed salts and free acid forms can also be used.

Alternatively, modified oligonucleotide backbones that do not include a phosphorus atom therein have backbones that are formed by short chain alkyl or cycloalkyl internucleoside linkages, mixed heteroatom and alkyl or cycloalkyl internucleoside linkages, or one or more short chain heteroatomic or heterocyclic internucleoside linkages. These include those having morpholino linkages (formed in part from the sugar portion of a nucleoside); siloxane backbones; sulfide, sulfoxide and sulfone backbones; formacetyl and thioformacetyl backbones; methylene formacetyl and thioformacetyl backbones; alkene containing backbones; sulfamate backbones; methyleneimino and methylenehydrazino backbones; sulfonate and sulfonamide backbones; amide backbones; and others having mixed N, O, S and CH₂ component parts, as disclosed in U.S. Pat. Nos. 5,034,506; 5,166,315; 5,185,444; 5,214,134; 5,216,141; 5,235,033; 5,264,562; 5,264,564; 5,405,938; 5,434,257; 5,466,677; 5,470,967; 5,489,677; 5,541,307; 5,561,225; 5,596,086; 5,602,240; 5,610,289; 5,602,240; 5,608,046; 5,610,289; 5,618,704; 5,623, 070; 5,663,312; 5,633,360; 5,677,437; and 5,677,439.

Other oligonucleotides which can be used according to the present invention, are those modified in both sugar and the internucleoside linkage, i.e., the backbone, of the nucleotide units are replaced with novel groups. The base units are maintained for complementation with the appropriate polynucleotide target. An example for such an oligonucleotide mimetic, includes peptide nucleic acid (PNA). United States patents that teach the preparation of PNA compounds include, but are not limited to, U.S. Pat. Nos. 5,539,082; 5,714,331; and 5,719,262, each of which is herein incorporated by reference. Other backbone modifications, which can be used in the present invention are disclosed in U.S. Pat. No. 6,303,374.

Oligonucleotides of the present invention may also include base modifications or substitutions. As used herein, “unmodified” or “natural” bases include the purine bases adenine (A) and guanine (G), and the pyrimidine bases thymine (T), cytosine (C) and uracil (U). Modified bases include but are not limited to other synthetic and natural bases such as 5-methylcytosine (5-me-C), 5-hydroxymethyl cytosine, xanthine, hypoxanthine, 2-aminoadenine, 6-methyl and other alkyl derivatives of adenine and guanine, 2-propyl and other alkyl derivatives of adenine and guanine, 2-thiouracil, 2-thiothymine and 2-thiocytosine, 5-halouracil and cytosine, 5-propynyl uracil and cytosine, 6-azo uracil, cytosine and thymine, 5-uracil (pseudouracil), 4-thiouracil, 8-halo, 8-amino, 8-thiol, 8-thioalkyl, 8-hydroxyl and other 8-substituted adenines and guanines, 5-halo particularly 5-bromo, 5-trifluoromethyl and other 5-substituted uracils and cytosines, 7-methylguanine and 7-methyladenine, 8-azaguanine and 8-azaadenine, 7-deazaguanine and 7-deazaadenine and 3-deazaguanine and 3-deazaadenine. Further bases particularly useful for increasing the binding affinity of the oligomeric compounds of the invention include 5-substituted pyrimidines, 6-azapyrimidines and N-2, N-6 and O-6 substituted purines, including 2-aminopropyladenine, 5-propynyluracil and 5-propynylcytosine. 5-methylcytosine substitutions have been shown to increase nucleic acid duplex stability by 0.6-1.2° C. and are presently preferred base substitutions, even more particularly when combined with 2′-O-methoxyethyl sugar modifications.

Another modification of the oligonucleotides of the invention involves chemically linking to the oligonucleotide one or more moieties or conjugates, which enhance the activity, cellular distribution or cellular uptake of the oligonucleotide. Such moieties include but are not limited to lipid moieties such as a cholesterol moiety, cholic acid, a thioether, e.g., hexyl-S-tritylthiol, a thiocholesterol, an aliphatic chain, e.g., dodecandiol or undecyl residues, a phospholipid, e.g., di-hexadecyl-rac-glycerol or triethylammonium 1,2-di-O-hexadecyl-rac-glycero-3-H-phosphonate, a polyamine or a polyethylene glycol chain, or adamantane acetic acid, a palmityl moiety, or an octadecylamine or hexylamino-carbonyl-oxycholesterol moiety, as disclosed in U.S. Pat. No. 6,303,374.

It is not necessary for all positions in a given oligonucleotide molecule to be uniformly modified, and in fact more than one of the aforementioned modifications may be incorporated in a single compound or even at a single nucleoside within an oligonucleotide.

It will be appreciated that oligonucleotides of the present invention may include further modifications for more efficient use as diagnostic agents and/or to increase bioavailability, therapeutic efficacy and reduce cytotoxicity.

To enable cellular expression of the polynucleotides of the present invention, a nucleic acid construct according to the present invention may be used, which includes at least a coding region of one of the above nucleic acid sequences, and further includes at least one cis acting regulatory element. As used herein, the phrase “cis acting regulatory element” refers to a polynucleotide sequence, preferably a promoter, which binds a trans acting regulator and regulates the transcription of a coding sequence located downstream thereto.

Any suitable promoter sequence can be used by the nucleic acid construct of the present invention.

Preferably, the promoter utilized by the nucleic acid construct of the present invention is active in the specific cell population transformed. Examples of cell type-specific and/or tissue-specific promoters include promoters such as albumin that is liver specific, lymphoid specific promoters [Calame et al., (1988) Adv. Immunol. 43:235-275]; in particular promoters of T-cell receptors [Winoto et al., (1989) EMBO J. 8:729-733] and immunoglobulins; [Banerji et al. (1983) Cell 33729-740], neuron-specific promoters such as the neurofilament promoter [Byrne et al. (1989) Proc. Natl. Acad. Sci. USA 86:5473-5477], pancreas-specific promoters [Edlunch et al. (1985) Science 230:912-916] or mammary gland-specific promoters such as the milk whey promoter (U.S. Pat. No. 4,873,316 and European Application Publication No. 264,166). The nucleic acid construct of the present invention can further include an enhancer, which can be adjacent or distant to the promoter sequence and can function in up regulating the transcription therefrom.

The nucleic acid construct of the present invention preferably further includes an appropriate selectable marker and/or an origin of replication. Preferably, the nucleic acid construct utilized is a shuttle vector, which can propagate both in E. coli (wherein the construct comprises an appropriate selectable marker and origin of replication) and be compatible for propagation in cells, or integration in a gene and a tissue of choice. The construct according to the present invention can be, for example, a plasmid, a bacmid, a phagemid, a cosmid, a phage, a virus or an artificial chromosome.

Examples of suitable constructs include, but are not limited to, pcDNA3, pcDNA3.1 (+/−), pGL3, PzeoSV2 (+/−), pDisplay, pEF/myc/cyto, pCMV/myc/cyto each of which is commercially available from Invitrogen Co. (invitrogen.com). Examples of retroviral vector and packaging systems are those sold by Clontech, San Diego, Calif., including Retro-X vectors pLNCX and pLXSN, which permit cloning into multiple cloning sites and the transgene is transcribed from CMV promoter. Vectors derived from Mo-MuLV are also included such as pBabe, where the transgene will be transcribed from the 5′LTR promoter.

Currently preferred in vivo nucleic acid transfer techniques include transfection with viral or non-viral constructs, such as adenovirus, lentivirus, Herpes simplex I virus, or adeno-associated virus (AAV) and lipid-based systems. Useful lipids for lipid-mediated transfer of the gene are, for example, DOTMA, DOPE, and DC-Chol [Tonkinson et al., Cancer Investigation, 14(1): 54-65 (1996)]. The most preferred constructs for use in gene therapy are viruses, most preferably adenoviruses, AAV, lentiviruses, or retroviruses. A viral construct such as a retroviral construct includes at least one transcriptional promoter/enhancer or locus-defining element(s), or other elements that control gene expression by other means such as alternate splicing, nuclear RNA export, or post-translational modification of messenger. Such vector constructs also include a packaging signal, long terminal repeats (LTRs) or portions thereof, and positive and negative strand primer binding sites appropriate to the virus used, unless it is already present in the viral construct. In addition, such a construct typically includes a signal sequence for secretion of the peptide from a host cell in which it is placed. Preferably the signal sequence for this purpose is a mammalian signal sequence or the signal sequence of the polypeptide variants of the present invention. Optionally, the construct may also include a signal that directs polyadenylation, as well as one or more restriction sites and a translation termination sequence. By way of example, such constructs will typically include a 5′ LTR, a tRNA binding site, a packaging signal, an origin of second-strand DNA synthesis, and a 3′ LTR or a portion thereof. Other vectors can be used that are non-viral, such as cationic lipids, polylysine, and dendrimers.

Hybridization Assays

Detection of a nucleic acid of interest in a biological sample may optionally be effected by hybridization-based assays using an oligonucleotide probe (non-limiting examples of probes according to the present invention were previously described).

Traditional hybridization assays include PCR, RT-PCR, Real-time PCR, RNase protection, in-situ hybridization, primer extension, Southern blots (DNA detection), dot or slot blots (DNA, RNA), and Northern blots (RNA detection) (NAT type assays are described in greater detail below). More recently, PNAs have been described (Nielsen et al. 1999, Current Opin. Biotechnol. 10:71-75). Other detection methods include kits containing probes on a dipstick setup and the like.

Hybridization based assays which allow the detection of a variant of interest (i.e., DNA or RNA) in a biological sample rely on the use of oligonucleotides which can be 10, 15, 20, or 30 to 100 nucleotides long preferably from 10 to 50, more preferably from 40 to 50 nucleotides long.

Thus, the isolated polynucleotides (oligonucleotides) of the present invention are preferably hybridizable with any of the herein described nucleic acid sequences under moderate to stringent hybridization conditions.

Moderate to stringent hybridization conditions are characterized by a hybridization solution such as containing 10% dextrane sulfate, 1 M NaCl, 1% SDS and 5×10⁶ cpm ³²P labeled probe, at 65° C., with a final wash solution of 0.2×SSC and 0.1% SDS and final wash at 65° C. and whereas moderate hybridization is effected using a hybridization solution containing 10% dextrane sulfate, 1 M NaCl, 1% SDS and 5×10⁶ cpm ³²P labeled probe, at 65° C., with a final wash solution of 1×SSC and 0.1% SDS and final wash at 50° C.

More generally, hybridization of short nucleic acids (below 200 bp in length, e.g. 17-40 bp in length) can be effected using the following exemplary hybridization protocols which can be modified according to the desired stringency; (i) hybridization solution of 6×SSC and 1% SDS or 3 M TMACI, 0.01 M sodium phosphate (pH 6.8), 1 mM EDTA (pH 7.6), 0.5% SDS, 100 μg/ml denatured salmon sperm DNA and 0.1% nonfat dried milk, hybridization temperature of 1-1.5° C. below the T_(m), final wash solution of 3 M TMACI, 0.01 M sodium phosphate (pH 6.8), 1 mM EDTA (pH 7.6), 0.5% SDS at 1-1.5° C. below the T_(m); (ii) hybridization solution of 6×SSC and 0.1% SDS or 3 M TMACI, 0.01 M sodium phosphate (pH 6.8), 1 mM EDTA (pH 7.6), 0.5% SDS, 100 μg/ml denatured salmon sperm DNA and 0.1% nonfat dried milk, hybridization temperature of 2-2.5° C. below the T_(m), final wash solution of 3 M TMACI, 0.01 M sodium phosphate (pH 6.8), 1 mM EDTA (pH 7.6), 0.5% SDS at 1-1.5° C. below the T_(m), final wash solution of 6×SSC, and final wash at 22° C.; (iii) hybridization solution of 6×SSC and 1% SDS or 3 M TMACI, 0.01 M sodium phosphate (pH 6.8), 1 mM EDTA (pH 7.6), 0.5% SDS, 100 μg/ml denatured salmon sperm DNA and 0.1% nonfat dried milk, hybridization temperature.

The detection of hybrid duplexes can be carried out by a number of methods. Typically, hybridization duplexes are separated from unhybridized nucleic acids and the labels bound to the duplexes are then detected. Such labels refer to radioactive, fluorescent, biological or enzymatic tags or labels of standard use in the art. A label can be conjugated to either the oligonucleotide probes or the nucleic acids derived from the biological sample.

Probes can be labeled according to numerous well known methods. Non-limiting examples of radioactive labels include 3H, 14C, 32P, and 35S. Non-limiting examples of detectable markers include ligands, fluorophores, chemiluminescent agents, enzymes, and antibodies. Other detectable markers for use with probes, which can enable an increase in sensitivity of the method of the invention, include biotin and radio-nucleotides. It will become evident to the person of ordinary skill that the choice of a particular label dictates the manner in which it is bound to the probe.

For example, oligonucleotides of the present invention can be labeled subsequent to synthesis, by incorporating biotinylated dNTPs or rNTP, or some similar means (e.g., photo-cross-linking a psoralen derivative of biotin to RNAs), followed by addition of labeled streptavidin (e.g., phycoerythrin-conjugated streptavidin) or the equivalent. Alternatively, when fluorescently-labeled oligonucleotide probes are used, fluorescein, lissamine, phycoerythrin, rhodamine (Perkin Elmer Cetus), Cy2, Cy3, Cy3.5, Cy5, Cy5.5, Cy7, FluorX (Amersham) and others [e.g., Kricka et al. (1992), Academic Press San Diego, Calif] can be attached to the oligonucleotides.

Those skilled in the art will appreciate that wash steps may be employed to wash away excess target DNA or probe as well as unbound conjugate. Further, standard heterogeneous assay formats are suitable for detecting the hybrids using the labels present on the oligonucleotide primers and probes.

It will be appreciated that a variety of controls may be usefully employed to improve accuracy of hybridization assays. For instance, samples may be hybridized to an irrelevant probe and treated with RNAse A prior to hybridization, to assess false hybridization.

Although the present invention is not specifically dependent on the use of a label for the detection of a particular nucleic acid sequence, such a label might be beneficial, by increasing the sensitivity of the detection. Furthermore, it enables automation. Probes can be labeled according to numerous well known methods.

As commonly known, radioactive nucleotides can be incorporated into probes of the invention by several methods. Non-limiting examples of radioactive labels include ³H, ¹⁴C, ³²P, and ³⁵S.

Those skilled in the art will appreciate that wash steps may be employed to wash away excess target DNA or probe as well as unbound conjugate. Further, standard heterogeneous assay formats are suitable for detecting the hybrids using the labels present on the oligonucleotide primers and probes.

It will be appreciated that a variety of controls may be usefully employed to improve accuracy of hybridization assays.

Probes of the invention can be utilized with naturally occurring sugar-phosphate backbones as well as modified backbones including phosphorothioates, dithionates, alkyl phosphonates and a-nucleotides and the like. Probes of the invention can be constructed of either ribonucleic acid (RNA) or deoxyribonucleic acid (DNA), and preferably of DNA.

NAT Assays

Detection of a nucleic acid of interest in a biological sample may also optionally be effected by NAT-based assays, which involve nucleic acid amplification technology, such as PCR for example (or variations thereof such as real-time PCR for example).

As used herein, a “primer” defines an oligonucleotide which is capable of annealing to (hybridizing with) a target sequence, thereby creating a double stranded region which can serve as an initiation point for DNA synthesis under suitable conditions.

Amplification of a selected, or target, nucleic acid sequence may be carried out by a number of suitable methods. See generally Kwoh et al., 1990, Am. Biotechnol. Lab. 8:14 Numerous amplification techniques have been described and can be readily adapted to suit particular needs of a person of ordinary skill. Non-limiting examples of amplification techniques include polymerase chain reaction (PCR), ligase chain reaction (LCR), strand displacement amplification (SDA), transcription-based amplification, the q3 replicase system and NASBA (Kwoh et al., 1989, Proc. Natl. Acad. Sci. USA 86, 1173-1177; Lizardi et al., 1988, BioTechnology 6:1197-1202; Malek et al., 1994, Methods Mol. Biol., 28:253-260; and Sambrook et al., 1989, supra).

The terminology “amplification pair” (or “primer pair”) refers herein to a pair of oligonucleotides (oligos) of the present invention, which are selected to be used together in amplifying a selected nucleic acid sequence by one of a number of types of amplification processes, preferably a polymerase chain reaction. Other types of amplification processes include ligase chain reaction, strand displacement amplification, or nucleic acid sequence-based amplification, as explained in greater detail below. As commonly known in the art, the oligos are designed to bind to a complementary sequence under selected conditions.

In one particular embodiment, amplification of a nucleic acid sample from a patient is amplified under conditions which favor the amplification of the most abundant differentially expressed nucleic acid. In one preferred embodiment, RT-PCR is carried out on an mRNA sample from a patient under conditions which favor the amplification of the most abundant mRNA. In another preferred embodiment, the amplification of the differentially expressed nucleic acids is carried out simultaneously. It will be realized by a person skilled in the art that such methods could be adapted for the detection of differentially expressed proteins instead of differentially expressed nucleic acid sequences.

The nucleic acid (i.e. DNA or RNA) for practicing the present invention may be obtained according to well known methods.

Oligonucleotide primers of the present invention may be of any suitable length, depending on the particular assay format and the particular needs and targeted genomes employed. Optionally, the oligonucleotide primers are at least 12 nucleotides in length, preferably between 15 and 24 molecules, and they may be adapted to be especially suited to a chosen nucleic acid amplification system. As commonly known in the art, the oligonucleotide primers can be designed by taking into consideration the melting point of hybridization thereof with its targeted sequence (Sambrook et al., 1989, Molecular Cloning—A Laboratory Manual, 2nd Edition, CSH Laboratories; Ausubel et al., 1989, in Current Protocols in Molecular Biology, John Wiley & Sons Inc., N.Y.).

It will be appreciated that antisense oligonucleotides may be employed to quantify expression of a splice isoform of interest. Such detection is effected at the pre-mRNA level. Essentially the ability to quantitate transcription from a splice site of interest can be effected based on splice site accessibility. Oligonucleotides may compete with splicing factors for the splice site sequences. Thus, low activity of the antisense oligonucleotide is indicative of splicing activity.

The polymerase chain reaction and other nucleic acid amplification reactions are well known in the art (various non-limiting examples of these reactions are described in greater detail below). The pair of oligonucleotides according to this aspect of the present invention are preferably selected to have compatible melting temperatures (Tm), e.g., melting temperatures which differ by less than that 7° C., preferably less than 5° C., more preferably less than 4° C., most preferably less than 3° C., ideally between 3° C. and 0° C.

Polymerase Chain Reaction (PCR): The polymerase chain reaction (PCR), as described in U.S. Pat. Nos. 4,683,195 and 4,683,202 to Mullis and Mullis et al., is a method of increasing the concentration of a segment of target sequence in a mixture of genomic DNA without cloning or purification. This technology provides one approach to the problems of low target sequence concentration. PCR can be used to directly increase the concentration of the target to an easily detectable level. This process for amplifying the target sequence involves the introduction of a molar excess of two oligonucleotide primers which are complementary to their respective strands of the double-stranded target sequence to the DNA mixture containing the desired target sequence. The mixture is denatured and then allowed to hybridize. Following hybridization, the primers are extended with polymerase so as to form complementary strands. The steps of denaturation, hybridization (annealing), and polymerase extension (elongation) can be repeated as often as needed, in order to obtain relatively high concentrations of a segment of the desired target sequence.

The length of the segment of the desired target sequence is determined by the relative positions of the primers with respect to each other, and, therefore, this length is a controllable parameter. Because the desired segments of the target sequence become the dominant sequences (in terms of concentration) in the mixture, they are said to be “PCR-amplified.”

Ligase Chain Reaction (LCR or LAR): The ligase chain reaction [LCR; sometimes referred to as “Ligase Amplification Reaction” (LAR)] has developed into a well-recognized alternative method of amplifying nucleic acids. In LCR, four oligonucleotides, two adjacent oligonucleotides which uniquely hybridize to one strand of target DNA, and a complementary set of adjacent oligonucleotides, which hybridize to the opposite strand are mixed and DNA ligase is added to the mixture. Provided that there is complete complementarity at the junction, ligase will covalently link each set of hybridized molecules. Importantly, in LCR, two probes are ligated together only when they base-pair with sequences in the target sample, without gaps or mismatches. Repeated cycles of denaturation, and ligation amplify a short segment of DNA. LCR has also been used in combination with PCR to achieve enhanced detection of single-base changes: see for example Segev, PCT Publication No. WO9001069 A1 (1990). However, because the four oligonucleotides used in this assay can pair to form two short ligatable fragments, there is the potential for the generation of target-independent background signal. The use of LCR for mutant screening is limited to the examination of specific nucleic acid positions.

Self-Sustained Synthetic Reaction (3SR/NASBA): The self-sustained sequence replication reaction (3SR) is a transcription-based in vitro amplification system that can exponentially amplify RNA sequences at a uniform temperature. The amplified RNA can then be utilized for mutation detection. In this method, an oligonucleotide primer is used to add a phage RNA polymerase promoter to the 5′ end of the sequence of interest. In a cocktail of enzymes and substrates that includes a second primer, reverse transcriptase, RNase H, RNA polymerase and ribo- and deoxyribonucleoside triphosphates, the target sequence undergoes repeated rounds of transcription, cDNA synthesis and second-strand synthesis to amplify the area of interest. The use of 3SR to detect mutations is kinetically limited to screening small segments of DNA (e.g., 200-300 base pairs).

Q-Beta (Qβ) Replicase: In this method, a probe which recognizes the sequence of interest is attached to the replicatable RNA template for Qβ replicase. A previously identified major problem with false positives resulting from the replication of unhybridized probes has been addressed through use of a sequence-specific ligation step. However, available thermostable DNA ligases are not effective on this RNA substrate, so the ligation must be performed by T4 DNA ligase at low temperatures (37 degrees C.). This prevents the use of high temperature as a means of achieving specificity as in the LCR, the ligation event can be used to detect a mutation at the junction site, but not elsewhere.

A successful diagnostic method must be very specific. A straight-forward method of controlling the specificity of nucleic acid hybridization is by controlling the temperature of the reaction. While the 3SR/NASBA, and Qβ systems are all able to generate a large quantity of signal, one or more of the enzymes involved in each cannot be used at high temperature (i.e., >55 degrees C.). Therefore the reaction temperatures cannot be raised to prevent non-specific hybridization of the probes. If probes are shortened in order to make them melt more easily at low temperatures, the likelihood of having more than one perfect match in a complex genome increases. For these reasons, PCR and LCR currently dominate the research field in detection technologies.

The basis of the amplification procedure in the PCR and LCR is the fact that the products of one cycle become usable templates in all subsequent cycles, consequently doubling the population with each cycle. The final yield of any such doubling system can be expressed as: (1+X)^(n)=y, where “X” is the mean efficiency (percent copied in each cycle), “n” is the number of cycles, and “y” is the overall efficiency, or yield of the reaction. If every copy of a target DNA is utilized as a template in every cycle of a polymerase chain reaction, then the mean efficiency is 100%. If 20 cycles of PCR are performed, then the yield will be 2²⁰, or 1,048,576 copies of the starting material. If the reaction conditions reduce the mean efficiency to 85%, then the yield in those 20 cycles will be only 1.85²⁰, or 220,513 copies of the starting material. In other words, a PCR running at 85% efficiency will yield only 21% as much final product, compared to a reaction running at 100% efficiency. A reaction that is reduced to 50% mean efficiency will yield less than 1% of the possible product.

In practice, routine polymerase chain reactions rarely achieve the theoretical maximum yield, and PCRs are usually run for more than 20 cycles to compensate for the lower yield. At 50% mean efficiency, it would take 34 cycles to achieve the million-fold amplification theoretically possible in 20, and at lower efficiencies, the number of cycles required becomes prohibitive. In addition, any background products that amplify with a better mean efficiency than the intended target will become the dominant products.

Also, many variables can influence the mean efficiency of PCR, including target DNA length and secondary structure, primer length and design, primer and dNTP concentrations, and buffer composition, to name but a few. Contamination of the reaction with exogenous DNA (e.g., DNA spilled onto lab surfaces) or cross-contamination is also a major consideration. Reaction conditions must be carefully optimized for each different primer pair and target sequence, and the process can take days, even for an experienced investigator. The laboriousness of this process, including numerous technical considerations and other factors, presents a significant drawback to using PCR in the clinical setting. Indeed, PCR has yet to penetrate the clinical market in a significant way. The same concerns arise with LCR, as LCR must also be optimized to use different oligonucleotide sequences for each target sequence. In addition, both methods require expensive equipment, capable of precise temperature cycling.

Many applications of nucleic acid detection technologies, such as in studies of allelic variation, involve not only detection of a specific sequence in a complex background, but also the discrimination between sequences with few, or single, nucleotide differences. One method of the detection of allele-specific variants by PCR is based upon the fact that it is difficult for Taq polymerase to synthesize a DNA strand when there is a mismatch between the template strand and the 3′ end of the primer. An allele-specific variant may be detected by the use of a primer that is perfectly matched with only one of the possible alleles; the mismatch to the other allele acts to prevent the extension of the primer, thereby preventing the amplification of that sequence. This method has a substantial limitation in that the base composition of the mismatch influences the ability to prevent extension across the mismatch, and certain mismatches do not prevent extension or have only a minimal effect.

A similar 3′-mismatch strategy is used with greater effect to prevent ligation in the LCR. Any mismatch effectively blocks the action of the thermostable ligase, but LCR still has the drawback of target-independent background ligation products initiating the amplification. Moreover, the combination of PCR with subsequent LCR to identify the nucleotides at individual positions is also a clearly cumbersome proposition for the clinical laboratory.

The direct detection method according to various preferred embodiments of the present invention may be, for example a cycling probe reaction (CPR) or a branched DNA analysis.

When a sufficient amount of a nucleic acid to be detected is available, there are advantages to detecting that sequence directly, instead of making more copies of that target, (e.g., as in PCR and LCR). Most notably, a method that does not amplify the signal exponentially is more amenable to quantitative analysis. Even if the signal is enhanced by attaching multiple dyes to a single oligonucleotide, the correlation between the final signal intensity and amount of target is direct. Such a system has an additional advantage that the products of the reaction will not themselves promote further reaction, so contamination of lab surfaces by the products is not as much of a concern. Recently devised techniques have sought to eliminate the use of radioactivity and/or improve the sensitivity in automatable formats. Two examples are the “Cycling Probe Reaction” (CPR), and “Branched DNA” (bDNA).

Cycling probe reaction (CPR): The cycling probe reaction (CPR), uses a long chimeric oligonucleotide in which a central portion is made of RNA while the two termini are made of DNA. Hybridization of the probe to a target DNA and exposure to a thermostable RNase H causes the RNA portion to be digested. This destabilizes the remaining DNA portions of the duplex, releasing the remainder of the probe from the target DNA and allowing another probe molecule to repeat the process. The signal, in the form of cleaved probe molecules, accumulates at a linear rate. While the repeating process increases the signal, the RNA portion of the oligonucleotide is vulnerable to RNases that may carried through sample preparation.

Branched DNA: Branched DNA (bDNA), involves oligonucleotides with branched structures that allow each individual oligonucleotide to carry 35 to 40 labels (e.g., alkaline phosphatase enzymes). While this enhances the signal from a hybridization event, signal from non-specific binding is similarly increased.

The detection of at least one sequence change according to various preferred embodiments of the present invention may be accomplished by, for example restriction fragment length polymorphism (RFLP analysis), allele specific oligonucleotide (ASO) analysis, Denaturing/Temperature Gradient Gel Electrophoresis (DGGE/TGGE), Single-Strand Conformation Polymorphism (SSCP) analysis or Dideoxy fingerprinting (ddF).

The demand for tests which allow the detection of specific nucleic acid sequences and sequence changes is growing rapidly in clinical diagnostics. As nucleic acid sequence data for genes from humans and pathogenic organisms accumulates, the demand for fast, cost-effective, and easy-to-use tests for as yet mutations within specific sequences is rapidly increasing.

A handful of methods have been devised to scan nucleic acid segments for mutations. One option is to determine the entire gene sequence of each test sample (e.g., a bacterial isolate). For sequences under approximately 600 nucleotides, this may be accomplished using amplified material (e.g., PCR reaction products). This avoids the time and expense associated with cloning the segment of interest. However, specialized equipment and highly trained personnel are required, and the method is too labor-intense and expensive to be practical and effective in the clinical setting.

In view of the difficulties associated with sequencing, a given segment of nucleic acid may be characterized on several other levels. At the lowest resolution, the size of the molecule can be determined by electrophoresis by comparison to a known standard run on the same gel. A more detailed picture of the molecule may be achieved by cleavage with combinations of restriction enzymes prior to electrophoresis, to allow construction of an ordered map. The presence of specific sequences within the fragment can be detected by hybridization of a labeled probe, or the precise nucleotide sequence can be determined by partial chemical degradation or by primer extension in the presence of chain-terminating nucleotide analogs.

Restriction fragment length polymorphism (RFLP): For detection of single-base differences between like sequences, the requirements of the analysis are often at the highest level of resolution. For cases in which the position of the nucleotide in question is known in advance, several methods have been developed for examining single base changes without direct sequencing. For example, if a mutation of interest happens to fall within a restriction recognition sequence, a change in the pattern of digestion can be used as a diagnostic tool (e.g., restriction fragment length polymorphism [RFLP] analysis).

Single point mutations have been also detected by the creation or destruction of RFLPs. Mutations are detected and localized by the presence and size of the RNA fragments generated by cleavage at the mismatches. Single nucleotide mismatches in DNA heteroduplexes are also recognized and cleaved by some chemicals, providing an alternative strategy to detect single base substitutions, generically named the “Mismatch Chemical Cleavage” (MCC). However, this method requires the use of osmium tetroxide and piperidine, two highly noxious chemicals which are not suited for use in a clinical laboratory.

RFLP analysis suffers from low sensitivity and requires a large amount of sample. When RFLP analysis is used for the detection of point mutations, it is, by its nature, limited to the detection of only those single base changes which fall within a restriction sequence of a known restriction endonuclease. Moreover, the majority of the available enzymes have 4 to 6 base-pair recognition sequences, and cleave too frequently for many large-scale DNA manipulations. Thus, it is applicable only in a small fraction of cases, as most mutations do not fall within such sites.

A handful of rare-cutting restriction enzymes with 8 base-pair specificities have been isolated and these are widely used in genetic mapping, but these enzymes are few in number, are limited to the recognition of G+C-rich sequences, and cleave at sites that tend to be highly clustered. Recently, endonucleases encoded by group I introns have been discovered that might have greater than 12 base-pair specificity, but again, these are few in number.

Allele specific oligonucleotide (ASO): If the change is not in a recognition sequence, then allele-specific oligonucleotides (ASOs), can be designed to hybridize in proximity to the mutated nucleotide, such that a primer extension or ligation event can bused as the indicator of a match or a mis-match. Hybridization with radioactively labeled allelic specific oligonucleotides (ASO) also has been applied to the detection of specific point mutations. The method is based on the differences in the melting temperature of short DNA fragments differing by a single nucleotide. Stringent hybridization and washing conditions can differentiate between mutant and wild-type alleles. The ASO approach applied to PCR products also has been extensively utilized by various researchers to detect and characterize point mutations in ras genes and gsp/gip oncogenes. Because of the presence of various nucleotide changes in multiple positions, the ASO method requires the use of many oligonucleotides to cover all possible oncogenic mutations.

With either of the techniques described above (i.e., RFLP and ASO), the precise location of the suspected mutation must be known in advance of the test. That is to say, they are inapplicable when one needs to detect the presence of a mutation within a gene or sequence of interest.

Denaturing/Temperature Gradient Gel Electrophoresis (DGGE/TGGE): Two other methods rely on detecting changes in electrophoretic mobility in response to minor sequence changes. One of these methods, termed “Denaturing Gradient Gel Electrophoresis” (DGGE) is based on the observation that slightly different sequences will display different patterns of local melting when electrophoretically resolved on a gradient gel. In this manner, variants can be distinguished, as differences in melting properties of homoduplexes versus heteroduplexes differing in a single nucleotide can detect the presence of mutations in the target sequences because of the corresponding changes in their electrophoretic mobilities. The fragments to be analyzed, usually PCR products, are “clamped” at one end by a long stretch of G-C base pairs (30-80) to allow complete denaturation of the sequence of interest without complete dissociation of the strands. The attachment of a GC “clamp” to the DNA fragments increases the fraction of mutations that can be recognized by DGGE. Attaching a GC clamp to one primer is critical to ensure that the amplified sequence has a low dissociation temperature. Modifications of the technique have been developed, using temperature gradients, and the method can be also applied to RNA:RNA duplexes.

Limitations on the utility of DGGE include the requirement that the denaturing conditions must be optimized for each type of DNA to be tested. Furthermore, the method requires specialized equipment to prepare the gels and maintain the needed high temperatures during electrophoresis. The expense associated with the synthesis of the clamping tail on one oligonucleotide for each sequence to be tested is also a major consideration. In addition, long running times are required for DGGE. The long running time of DGGE was shortened in a modification of DGGE called constant denaturant gel electrophoresis (CDGE). CDGE requires that gels be performed under different denaturant conditions in order to reach high efficiency for the detection of mutations.

A technique analogous to DGGE, termed temperature gradient gel electrophoresis (TGGE), uses a thermal gradient rather than a chemical denaturant gradient. TGGE requires the use of specialized equipment which can generate a temperature gradient perpendicularly oriented relative to the electrical field. TGGE can detect mutations in relatively small fragments of DNA therefore scanning of large gene segments requires the use of multiple PCR products prior to running the gel.

Single-Strand Conformation Polymorphism (SSCP): Another common method, called “Single-Strand Conformation Polymorphism” (SSCP) was developed by Hayashi, Sekya and colleagues and is based on the observation that single strands of nucleic acid can take on characteristic conformations in non-denaturing conditions, and these conformations influence electrophoretic mobility. The complementary strands assume sufficiently different structures that one strand may be resolved from the other. Changes in sequences within the fragment will also change the conformation, consequently altering the mobility and allowing this to be used as an assay for sequence variations.

The SSCP process involves denaturing a DNA segment (e.g., a PCR product) that is labeled on both strands, followed by slow electrophoretic separation on a non-denaturing polyacrylamide gel, so that intra-molecular interactions can form and not be disturbed during the run. This technique is extremely sensitive to variations in gel composition and temperature. A serious limitation of this method is the relative difficulty encountered in comparing data generated in different laboratories, under apparently similar conditions.

Dideoxy fingerprinting (ddF): The dideoxy fingerprinting (ddF) is another technique developed to scan genes for the presence of mutations. The ddF technique combines components of Sanger dideoxy sequencing with SSCP. A dideoxy sequencing reaction is performed using one dideoxy terminator and then the reaction products are electrophoresed on nondenaturing polyacrylamide gels to detect alterations in mobility of the termination segments as in SSCP analysis. While ddF is an improvement over SSCP in terms of increased sensitivity, ddF requires the use of expensive dideoxynucleotides and this technique is still limited to the analysis of fragments of the size suitable for SSCP (i.e., fragments of 200-300 bases for optimal detection of mutations).

In addition to the above limitations, all of these methods are limited as to the size of the nucleic acid fragment that can be analyzed. For the direct sequencing approach, sequences of greater than 600 base pairs require cloning, with the consequent delays and expense of either deletion sub-cloning or primer walking, in order to cover the entire fragment. SSCP and DGGE have even more severe size limitations. Because of reduced sensitivity to sequence changes, these methods are not considered suitable for larger fragments. Although SSCP is reportedly able to detect 90% of single-base substitutions within a 200 base-pair fragment, the detection drops to less than 50% for 400 base pair fragments. Similarly, the sensitivity of DGGE decreases as the length of the fragment reaches 500 base-pairs. The ddF technique, as a combination of direct sequencing and SSCP, is also limited by the relatively small size of the DNA that can be screened.

According to a presently preferred embodiment of the present invention the step of searching for any of the nucleic acid sequences described here, in tumor cells or in cells derived from a cancer patient is effected by any suitable technique, including, but not limited to, nucleic acid sequencing, polymerase chain reaction, ligase chain reaction, self-sustained synthetic reaction, Qβ-Replicase, cycling probe reaction, branched DNA, restriction fragment length polymorphism analysis, mismatch chemical cleavage, heteroduplex analysis, allele-specific oligonucleotides, denaturing gradient gel electrophoresis, constant denaturant gel electrophoresis, temperature gradient gel electrophoresis and dideoxy fingerprinting.

Detection may also optionally be performed with a chip or other such device. The nucleic acid sample which includes the candidate region to be analyzed is preferably isolated, amplified and labeled with a reporter group. This reporter group can be a fluorescent group such as phycoerythrin. The labeled nucleic acid is then incubated with the probes immobilized on the chip using a fluidics station. describe the fabrication of fluidics devices and particularly microcapillary devices, in silicon and glass substrates.

Once the reaction is completed, the chip is inserted into a scanner and patterns of hybridization are detected. The hybridization data is collected, as a signal emitted from the reporter groups already incorporated into the nucleic acid, which is now bound to the probes attached to the chip. Since the sequence and position of each probe immobilized on the chip is known, the identity of the nucleic acid hybridized to a given probe can be determined.

It will be appreciated that when utilized along with automated equipment, the above described detection methods can be used to screen multiple samples for a disease and/or pathological condition both rapidly and easily.

Amino Acid Sequences and Peptides

The terms “polypeptide,” “peptide” and “protein” are used interchangeably herein to refer to a polymer of amino acid residues. The terms apply to amino acid polymers in which one or more amino acid residue is an analog or mimetic of a corresponding naturally occurring amino acid, as well as to naturally occurring amino acid polymers. Polypeptides can be modified, e.g., by the addition of carbohydrate residues to form glycoproteins. The terms “polypeptide,” “peptide” and “protein” include glycoproteins, as well as non-glycoproteins.

Polypeptide products can be biochemically synthesized such as by employing standard solid phase techniques. Such methods include but are not limited to exclusive solid phase synthesis, partial solid phase synthesis methods, fragment condensation, classical solution synthesis. These methods are preferably used when the peptide is relatively short (i.e., 10 kDa) and/or when it cannot be produced by recombinant techniques (i.e., not encoded by a nucleic acid sequence) and therefore involves different chemistry.

Solid phase polypeptide synthesis procedures are well known in the art and further described by John Morrow Stewart and Janis Dillaha Young, Solid Phase Peptide Syntheses (2nd Ed., Pierce Chemical Company, 1984).

Synthetic polypeptides can optionally be purified by preparative high performance liquid chromatography [Creighton T. (1983) Proteins, structures and molecular principles. WH Freeman and Co. N.Y.], after which their composition can be confirmed via amino acid sequencing.

In cases where large amounts of a polypeptide are desired, it can be generated using recombinant techniques such as described by Bitter et al., (1987) Methods in Enzymol. 153:516-544, Studier et al. (1990) Methods in Enzymol. 185:60-89, Brisson et al. (1984) Nature 310:511-514, Takamatsu et al. (1987) EMBO J. 6:307-311, Coruzzi et al. (1984) EMBO J. 3:1671-1680 and Brogli et al., (1984) Science 224:838-843, Gurley et al. (1986) Mol. Cell. Biol. 6:559-565 and Weissbach & Weissbach, 1988, Methods for Plant Molecular Biology, Academic Press, NY, Section VIII, pp 421-463.

The present invention also encompasses polypeptides encoded by the polynucleotide sequences of the present invention, as well as polypeptides according to the amino acid sequences described herein. The present invention also encompasses homologues of these polypeptides, such homologues can be at least 50%, at least 55%, at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 95% or more say 100% homologous to the amino acid sequences set forth below, as can be determined using BlastP software of the National Center of Biotechnology Information (NCBI) using default parameters, optionally and preferably including the following: filtering on (this option filters repetitive or low-complexity sequences from the query using the Seg (protein) program), scoring matrix is BLOSUM62 for proteins, word size is 3, E value is 10, gap costs are 11, 1 (initialization and extension), and number of alignments shown is 50. Nucleotide (nucleic acid) sequence homology/identity is preferably determined by using the BlastN software of the National Center of Biotechnology Information (NCBI) using default parameters, which preferably include using the DUST filter program, and also preferably include having an E value of 10, filtering low complexity sequences and a word size of 11. Finally, the present invention also encompasses fragments of the above described polypeptides and polypeptides having mutations, such as deletions, insertions or substitutions of one or more amino acids, either naturally occurring or artificially induced, either randomly or in a targeted fashion.

It will be appreciated that peptides identified according the present invention may be degradation products, synthetic peptides or recombinant peptides as well as peptidomimetics, typically, synthetic peptides and peptoids and semipeptoids which are peptide analogs, which may have, for example, modifications rendering the peptides more stable while in a body or more capable of penetrating into cells. Such modifications include, but are not limited to N terminus modification, C terminus modification, peptide bond modification, including, but not limited to, CH2-NH, CH2-S, CH2-S═O, O═C—NH, CH2-O, CH2-CH2, S═C—NH, CH═CH or CF═CH, backbone modifications, and residue modification. Methods for preparing peptidomimetic compounds are well known in the art and are specified. Further details in this respect are provided hereinunder.

Peptide bonds (—CO—NH—) within the peptide may be substituted, for example, by N-methylated bonds (—N(CH3)-CO—), ester bonds (—C(R)H—C—O—O—C(R)—N—), ketomethylen bonds (—CO—CH2-), α-aza bonds (—NH—N(R)—CO—), wherein R is any alkyl, e.g., methyl, carba bonds (—CH2-NH—), hydroxyethylene bonds (—CH(OH)—CH2-), thioamide bonds (—CS—NH—), olefinic double bonds (—CH═CH—), retro amide bonds (—NH—CO—), peptide derivatives (—N(R)—CH2-CO—), wherein R is the “normal” side chain, naturally presented on the carbon atom.

These modifications can occur at any of the bonds along the peptide chain and even at several (2-3) at the same time.

Natural aromatic amino acids, Trp, Tyr and Phe, may be substituted for synthetic non-natural acid such as Phenylglycine, TIC, naphthylelanine (Nol), ring-methylated derivatives of Phe, halogenated derivatives of Phe or o-methyl-Tyr.

In addition to the above, the peptides of the present invention may also include one or more modified amino acids or one or more non-amino acid monomers (e.g. fatty acids, complex carbohydrates etc).

As used herein in the specification and in the claims section below the term “amino acid” or “amino acids” is understood to include the 20 naturally occurring amino acids; those amino acids often modified post-translationally in vivo, including, for example, hydroxyproline, phosphoserine and phosphothreonine; and other unusual amino acids including, but not limited to, 2-aminoadipic acid, hydroxylysine, isodesmosine, nor-valine, nor-leucine and ornithine. Furthermore, the term “amino acid” includes both D- and L-amino acids.

Table 1 non-conventional or modified amino acids which can be used with the present invention.

TABLE 1 Non-conventional amino acid Code Non-conventional amino acid Code α-aminobutyric acid Abu L-N-methylalanine Nmala α-amino-α-methylbutyrate Mgabu L-N-methylarginine Nmarg aminocyclopropane- Cpro L-N-methylasparagine Nmasn Carboxylate L-N-methylaspartic acid Nmasp aminoisobutyric acid Aib L-N-methylcysteine Nmcys aminonorbornyl- Norb L-N-methylglutamine Nmgin Carboxylate L-N-methylglutamic acid Nmglu Cyclohexylalanine Chexa L-N-methylhistidine Nmhis Cyclopentylalanine Cpen L-N-methylisolleucine Nmile D-alanine Dal L-N-methylleucine Nmleu D-arginine Darg L-N-methyllysine Nmlys D-aspartic acid Dasp L-N-methylmethionine Nmmet D-cysteine Dcys L-N-methylnorleucine Nmnle D-glutamine Dgln L-N-methylnorvaline Nmnva D-glutamic acid Dglu L-N-methylornithine Nmorn D-histidine Dhis L-N-methylphenylalanine Nmphe D-isoleucine Dile L-N-methylproline Nmpro D-leucine Dleu L-N-methylserine Nmser D-lysine Dlys L-N-methylthreonine Nmthr D-methionine Dmet L-N-methyltryptophan Nmtrp D-ornithine Dorn L-N-methyltyrosine Nmtyr D-phenylalanine Dphe L-N-methylvaline Nmval D-proline Dpro L-N-methylethylglycine Nmetg D-serine Dser L-N-methyl-t-butylglycine Nmtbug D-threonine Dthr L-norleucine Nle D-tryptophan Dtrp L-norvaline Nva D-tyrosine Dtyr α-methyl-aminoisobutyrate Maib D-valine Dval α-methyl-γ-aminobutyrate Mgabu D-α-methylalanine Dmala α-methylcyclohexylalanine Mchexa D-α-methylarginine Dmarg α-methylcyclopentylalanine Mcpen D-α-methylasparagine Dmasn α-methyl-α-napthylalanine Manap D-α-methylaspartate Dmasp α-methylpenicillamine Mpen D-α-methylcysteine Dmcys N-(4-aminobutyl)glycine Nglu D-α-methylglutamine Dmgln N-(2-aminoethyl)glycine Naeg D-α-methylhistidine Dmhis N-(3-aminopropyl)glycine Norn D-α-methylisoleucine Dmile N-amino-α-methylbutyrate Nmaabu D-α-methylleucine Dmleu α-napthylalanine Anap D-α-methyllysine Dmlys N-benzylglycine Nphe D-α-methylmethionine Dmmet N-(2-carbamylethyl)glycine Ngln D-α-methylornithine Dmorn N-(carbamylmethyl)glycine Nasn D-α-methylphenylalanine Dmphe N-(2-carboxyethyl)glycine Nglu D-α-methylproline Dmpro N-(carboxymethyl)glycine Nasp D-α-methylserine Dmser N-cyclobutylglycine Ncbut D-α-methylthreonine Dmthr N-cycloheptylglycine Nchep D-α-methyltryptophan Dmtrp N-cyclohexylglycine Nchex D-α-methyltyrosine Dmty N-cyclodecylglycine Ncdec D-α-methylvaline Dmval N-cyclododeclglycine Ncdod D-α-methylalnine Dnmala N-cyclooctylglycine Ncoct D-α-methylarginine Dnmarg N-cyclopropylglycine Ncpro D-α-methylasparagine Dnmasn N-cycloundecylglycine Ncund D-α-methylasparatate Dnmasp N-(2,2-diphenylethyl)glycine Nbhm D-α-methylcysteine Dnmcys N-(3,3- Nbhe diphenylpropyl)glycine D-N-methylleucine Dnmleu N-(3-indolylyethyl) glycine Nhtrp D-N-methyllysine Dnmlys N-methyl-γ-aminobutyrate Nmgabu N- Nmchexa D-N-methylmethionine Dnmmet methylcyclohexylalanine D-N-methylornithine Dnmorn N-methylcyclopentylalanine Nmcpen N-methylglycine Nala D-N-methylphenylalanine Dnmphe N-methylaminoisobutyrate Nmaib D-N-methylproline Dnmpro N-(1-methylpropyl)glycine Nile D-N-methylserine Dnmser N-(2-methylpropyl)glycine Nile D-N-methylserine Dnmser N-(2-methylpropyl)glycine Nleu D-N-methylthreonine Dnmthr D-N-methyltryptophan Dnmtrp N-(1-methylethyl)glycine Nva D-N-methyltyrosine Dnmtyr N-methyla-napthylalanine Nmanap D-N-methylvaline Dnmval N-methylpenicillamine Nmpen γ-aminobutyric acid Gabu N-(p-hydroxyphenyl)glycine Nhtyr L-t-butylglycine Tbug N-(thiomethyl)glycine Ncys L-ethylglycine Etg penicillamine Pen L-homophenylalanine Hphe L-α-methylalanine Mala L-α-methylarginine Marg L-α-methylasparagine Masn L-α-methylaspartate Masp L-α-methyl-t-butylglycine Mtbug L-α-methylcysteine Mcys L-methylethylglycine Metg L-α-methylglutamine Mgln L-α-methylglutamate Mglu L-α-methylhistidine Mhis L-α-methylhomo Mhphe phenylalanine L-α-methylisoleucine Mile N-(2-methylthioethyl)glycine Nmet D-N-methylglutamine Dnmgln N-(3- Narg guanidinopropyl)glycine D-N-methylglutamate Dnmglu N-(1-hydroxyethyl)glycine Nthr D-N-methylhistidine Dnmhis N-(hydroxyethyl)glycine Nser D-N-methylisoleucine Dnmile N-(imidazolylethyl)glycine Nhis D-N-methylleucine Dnmleu N-(3-indolylyethyl)glycine Nhtrp D-N-methyllysine Dnmlys N-methyl-γ-aminobutyrate Nmgabu N- Nmchexa D-N-methylmethionine Dnmmet methylcyclohexylalanine D-N-methylornithine Dnmorn N-methylcyclopentylalanine Nmcpen N-methylglycine Nala D-N-methylphenylalanine Dnmphe N-methylaminoisobutyrate Nmaib D-N-methylproline Dnmpro N-(1-methylpropyl)glycine Nile D-N-methylserine Dnmser N-(2-methylpropyl)glycine Nleu D-N-methylthreonine Dnmthr D-N-methyltryptophan Dnmtrp N-(1-methylethyl)glycine Nval D-N-methyltyrosine Dnmtyr N-methyla-napthylalanine Nmanap D-N-methylvaline Dnmval N-methylpenicillamine Nmpen γ-aminobutyric acid Gabu N-(p-hydroxyphenyl)glycine Nhtyr L-t-butylglycine Tbug N-(thiomethyl)glycine Ncys L-ethylglycine Etg penicillamine Pen L-homophenylalanine Hphe L-α-methylalanine Mala L-α-methylarginine Marg L-α-methylasparagine Masn L-α-methylaspartate Masp L-α-methyl-t-butylglycine Mtbug L-α-methylcysteine Mcys L-methylethylglycine Metg L-α-methylglutamine Mgln L-α-methylglutamate Mglu L-α-methylhistidine Mhis L-α- Mhphe methylhomophenylalanine L-α-methylisoleucine Mile N-(2-methylthioethyl)glycine Nmet L-α-methylleucine Mleu L-α-methyllysine Mlys L-α-methylmethionine Mmet L-α-methylnorleucine Mnle L-α-methylnorvaline Mnva L-α-methylornithine Morn L-α-methylphenylalanine Mphe L-α-methylproline Mpro L-α-methylserine mser L-α-methylthreonine Mthr L-α-methylvaline Mtrp L-α-methyltyrosine Mtyr L-α-methylleucine Mval L-N- Nmhphe Nnbhm methylhomophenylalanine N-(N-(2,2-diphenylethyl) N-(N-(3,3-diphenylpropyl) carbamylmethyl-glycine Nnbhm carbamylmethyl(1)glycine Nnbhe 1-carboxy-1-(2,2-diphenyl Nmbc ethylamino)cyclopropane

Since the peptides of the present invention are preferably utilized in diagnostics which require the peptides to be in soluble form, the peptides of the present invention preferably include one or more non-natural or natural polar amino acids, including but not limited to serine and threonine which are capable of increasing peptide solubility due to their hydroxyl-containing side chain.

The peptides of the present invention are preferably utilized in a linear form, although it will be appreciated that in cases where cyclicization does not severely interfere with peptide characteristics, cyclic forms of the peptide can also be utilized.

The peptides of present invention can be biochemically synthesized such as by using standard solid phase techniques. These methods include exclusive solid phase synthesis well known in the art, partial solid phase synthesis methods, fragment condensation, classical solution synthesis. These methods are preferably used when the peptide is relatively short (i.e., 10 kDa) and/or when it cannot be produced by recombinant techniques (i.e., not encoded by a nucleic acid sequence) and therefore involves different chemistry.

Synthetic peptides can be purified by preparative high performance liquid chromatography and the composition of which can be confirmed via amino acid sequencing.

In cases where large amounts of the peptides of the present invention are desired, the peptides of the present invention can be generated using recombinant techniques such as described by Bitter et al., (1987) Methods in Enzymol. 153:516-544, Studier et al. (1990) Methods in Enzymol. 185:60-89, Brisson et al. (1984) Nature 310:511-514, Takamatsu et al. (1987) EMBO J. 6:307-311, Coruzzi et al. (1984) EMBO J. 3:1671-1680 and Brogli et al., (1984) Science 224:838-843, Gurley et al. (1986) Mol. Cell. Biol. 6:559-565 and Weissbach & Weissbach, 1988, Methods for Plant Molecular Biology, Academic Press, NY, Section VIII, pp 421-463 and also as described above.

Antibodies

“Antibody” refers to a polypeptide ligand that is preferably substantially encoded by an immunoglobulin gene or immunoglobulin genes, or fragments thereof, which specifically binds and recognizes an epitope (e.g., an antigen). The recognized immunoglobulin genes include the kappa and lambda light chain constant region genes, the alpha, gamma, delta, epsilon and mu heavy chain constant region genes, and the myriad-immunoglobulin variable region genes. Antibodies exist, e.g., as intact immunoglobulins or as a number of well characterized fragments produced by digestion with various peptidases. This includes, e.g., Fab′ and F(ab)′₂ fragments. The term “antibody,” as used herein, also includes antibody fragments either produced by the modification of whole antibodies or those synthesized de novo using recombinant DNA methodologies. It also includes polyclonal antibodies, monoclonal antibodies, chimeric antibodies, humanized antibodies, or single chain antibodies. “Fc” portion of an antibody refers to that portion of an immunoglobulin heavy chain that comprises one or more heavy chain constant region domains, CH1, CH2 and CH3, but does not include the heavy chain variable region.

The functional fragments of antibodies, such as Fab, F(ab′)2, and Fv that are capable of binding to macrophages, are described as follows: (1) Fab, the fragment which contains a monovalent antigen-binding fragment of an antibody molecule, can be produced by digestion of whole antibody with the enzyme papain to yield an intact light chain and a portion of one heavy chain; (2) Fab′, the fragment of an antibody molecule that can be obtained by treating whole antibody with pepsin, followed by reduction, to yield an intact light chain and a portion of the heavy chain; two Fab′ fragments are obtained per antibody molecule; (3) (Fab′)2, the fragment of the antibody that can be obtained by treating whole antibody with the enzyme pepsin without subsequent reduction; F(ab′)2 is a dimer of two Fab′ fragments held together by two disulfide bonds; (4) Fv, defined as a genetically engineered fragment containing the variable region of the light chain and the variable region of the heavy chain expressed as two chains; and (5) Single chain antibody (“SCA”), a genetically engineered molecule containing the variable region of the light chain and the variable region of the heavy chain, linked by a suitable polypeptide linker as a genetically fused single chain molecule.

Methods of producing polyclonal and monoclonal antibodies as well as fragments thereof are well known in the art (See for example, Harlow and Lane, Antibodies: A Laboratory Manual, Cold Spring Harbor Laboratory, New York, 1988, incorporated herein by reference).

Antibody fragments according to the present invention can be prepared by proteolytic hydrolysis of the antibody or by expression in E. coli or mammalian cells (e.g. Chinese hamster ovary cell culture or other protein expression systems) of DNA encoding the fragment. Antibody fragments can be obtained by pepsin or papain digestion of whole antibodies by conventional methods. For example, antibody fragments can be produced by enzymatic cleavage of antibodies with pepsin to provide a 5S fragment denoted F(ab′)2. This fragment can be further cleaved using a thiol reducing agent, and optionally a blocking group for the sulfhydryl groups resulting from cleavage of disulfide linkages, to produce 3.5 S Fab′ monovalent fragments. Alternatively, an enzymatic cleavage using pepsin produces two monovalent Fab′ fragments and an Fc fragment directly. These methods are described, for example, by Goldenberg, U.S. Pat. Nos. 4,036,945 and 4,331,647, and references contained therein, which patents are hereby incorporated by reference in their entirety. See also Porter, R. R. [Biochem. J. 73: 119-126 (1959)]. Other methods of cleaving antibodies, such as separation of heavy chains to form monovalent light-heavy chain fragments, further cleavage of fragments, or other enzymatic, chemical, or genetic techniques may also be used, so long as the fragments bind to the antigen that is recognized by the intact antibody.

Fv fragments comprise an association of VH and VL chains. This association may be noncovalent, as described in Inbar et al. [Proc. Nat'l Acad. Sci. USA 69:2659-62 (19720]. Alternatively, the variable chains can be linked by an intermolecular disulfide bond or cross-linked by chemicals such as glutaraldehyde. Preferably, the Fv fragments comprise VH and VL chains connected by a peptide linker. These single-chain antigen binding proteins (sFv) are prepared by constructing a structural gene comprising DNA sequences encoding the VH and VL domains connected by an oligonucleotide. The structural gene is inserted into an expression vector, which is subsequently introduced into a host cell such as E. coli. The recombinant host cells synthesize a single polypeptide chain with a linker peptide bridging the two V domains. Methods for producing sFvs are described, for example, by [Whitlow and Filpula, Methods 2: 97-105 (1991); Bird et al., Science 242:423-426 (1988); Pack et al., Bio/Technology 11:1271-77 (1993); and U.S. Pat. No. 4,946,778, which is hereby incorporated by reference in its entirety.

Another form of an antibody fragment is a peptide coding for a single complementarity-determining region (CDR). CDR peptides (“minimal recognition units”) can be obtained by constructing genes encoding the CDR of an antibody of interest. Such genes are prepared, for example, by using the polymerase chain reaction to synthesize the variable region from RNA of antibody-producing cells. See, for example, Larrick and Fry [Methods, 2: 106-10 (1991)].

Humanized forms of non-human (e.g., murine) antibodies are chimeric molecules of immunoglobulins, immunoglobulin chains or fragments thereof (such as Fv, Fab, Fab′, F(ab′) or other antigen-binding subsequences of antibodies) which contain minimal sequence derived from non-human immunoglobulin. Humanized antibodies include human immunoglobulins (recipient antibody) in which residues from a complementary determining region (CDR) of the recipient are replaced by residues from a CDR of a non-human species (donor antibody) such as mouse, rat or rabbit having the desired specificity, affinity and capacity. In some instances, Fv framework residues of the human immunoglobulin are replaced by corresponding non-human residues. Humanized antibodies may also comprise residues which are found neither in the recipient antibody nor in the imported CDR or framework sequences. In general, the humanized antibody will comprise substantially all of at least one, and typically two, variable domains, in which all or substantially all of the CDR regions correspond to those of a non-human immunoglobulin and all or substantially all of the FR regions are those of a human immunoglobulin consensus sequence. The humanized antibody optimally also will comprise at least a portion of an immunoglobulin constant region (Fc), typically that of a human immunoglobulin [Jones et al., Nature, 321:522-525 (1986); Riechmann et al., Nature, 332:323-329 (1988); and Presta, Curr. Op. Struct. Biol., 2:593-596 (1992)].

Methods for humanizing non-human antibodies are well known in the art. Generally, a humanized antibody has one or more amino acid residues introduced into it from a source which is non-human. These non-human amino acid residues are often referred to as import residues, which are typically taken from an import variable domain. Humanization can be essentially performed following the method of Winter and co-workers [Jones et al., Nature, 321:522-525 (1986); Riechmann et al., Nature 332:323-327 (1988); Verhoeyen et al., Science, 239:1534-1536 (1988)], by substituting rodent CDRs or CDR sequences for the corresponding sequences of a human antibody. Accordingly, such humanized antibodies are chimeric antibodies (U.S. Pat. No. 4,816,567), wherein substantially less than an intact human variable domain has been substituted by the corresponding sequence from a non-human species. In practice, humanized antibodies are typically human antibodies in which some CDR residues and possibly some FR residues are substituted by residues from analogous sites in rodent antibodies.

Human antibodies can also be produced using various techniques known in the art, including phage display libraries [Hoogenboom and Winter, J. Mol. Biol., 227:381 (1991); Marks et al., J. Mol. Biol., 222:581 (1991)]. The techniques of Cole et al. and Boemer et al. are also available for the preparation of human monoclonal antibodies (Cole et al., Monoclonal Antibodies and Cancer Therapy, Alan R. Liss, p. 77 (1985) and Boerner et al., J. Immunol., 147(1):86-95 (1991)]. Similarly, human antibodies can be made by introduction of human immunoglobulin loci into transgenic animals, e.g., mice in which the endogenous immunoglobulin genes have been partially or completely inactivated. Upon challenge, human antibody production is observed, which closely resembles that seen in humans in all respects, including gene rearrangement, assembly, and antibody repertoire. This approach is described, for example, in U.S. Pat. Nos. 5,545,807; 5,545,806; 5,569,825; 5,625,126; 5,633,425; 5,661,016, and in the following scientific publications: Marks et al., Bio/Technology 10,: 779-783 (1992); Lonberg et al., Nature 368: 856-859 (1994); Morrison, Nature 368 812-13 (1994); Fishwild et al., Nature Biotechnology 14, 845-51 (1996); Neuberger, Nature Biotechnology 14: 826 (1996); and Lonberg and Huszar, Intern. Rev. Immunol. 13, 65-93 (1995).

Preferably, the antibody of this aspect of the present invention specifically binds at least one epitope of the polypeptide variants of the present invention. As used herein, the term “epitope” refers to any antigenic determinant on an antigen to which the paratope of an antibody binds.

Epitopic determinants usually consist of chemically active surface groupings of molecules such as amino acids or carbohydrate side chains and usually have specific three dimensional structural characteristics, as well as specific charge characteristics.

Optionally, a unique epitope may be created in a variant due to a change in one or more post-translational modifications, including but not limited to glycosylation and/or phosphorylation, as described below. Such a change may also cause a new epitope to be created, for example through removal of glycosylation at a particular site.

An epitope according to the present invention may also optionally comprise part or all of a unique sequence portion of a variant according to the present invention in combination with at least one other portion of the variant which is not contiguous to the unique sequence portion in the linear polypeptide itself, yet which are able to form an epitope in combination. One or more unique sequence portions may optionally combine with one or more other non-contiguous portions of the variant (including a portion which may have high homology to a portion of the known protein) to form an epitope.

Immunoassays

In another embodiment of the present invention, an immunoassay can be used to qualitatively or quantitatively detect and analyze markers in a sample. This method comprises: providing an antibody that specifically binds to a marker; contacting a sample with the antibody; and detecting the presence of a complex of the antibody bound to the marker in the sample.

To prepare an antibody that specifically binds to a marker, purified protein markers can be used. Antibodies that specifically bind to a protein marker can be prepared using any suitable methods known in the art.

After the antibody is provided, a marker can be detected and/or quantified using any of a number of well recognized immunological binding assays. Useful assays include, for example, an enzyme immune assay (EIA) such as enzyme-linked immunosorbent assay (ELISA), a radioimmune assay (RIA), a Western blot assay, or a slot blot assay see, e.g., U.S. Pat. Nos. 4,366,241; 4,376,110; 4,517,288; and 4,837,168). Generally, a sample obtained from a subject can be contacted with the antibody that specifically binds the marker.

Optionally, the antibody can be fixed to a solid support to facilitate washing and subsequent isolation of the complex, prior to contacting the antibody with a sample. Examples of solid supports include but are not limited to glass or plastic in the form of, e.g., a microtiter plate, a stick, a bead, or a microbead. Antibodies can also be attached to a solid support.

After incubating the sample with antibodies, the mixture is washed and the antibody-marker complex formed can be detected. This can be accomplished by incubating the washed mixture with a detection reagent. Alternatively, the marker in the sample can be detected using an indirect assay, wherein, for example, a second, labeled antibody is used to detect bound marker-specific antibody, and/or in a competition or inhibition assay wherein, for example, a monoclonal antibody which binds to a distinct epitope of the marker are incubated simultaneously with the mixture.

Throughout the assays, incubation and/or washing steps may be required after each combination of reagents. Incubation steps can vary from about 5 seconds to several hours, preferably from about 5 minutes to about 24 hours. However, the incubation time will depend upon the assay format, marker, volume of solution, concentrations and the like. Usually the assays will be carried out at ambient temperature, although they can be conducted over a range of temperatures, such as 10° C. to 40° C.

The immunoassay can be used to determine a test amount of a marker in a sample from a subject. First, a test amount of a marker in a sample can be detected using the immunoassay methods described above. If a marker is present in the sample, it will form an antibody-marker complex with an antibody that specifically binds the marker under suitable incubation conditions described above. The amount of an antibody-marker complex can optionally be determined by comparing to a standard. As noted above, the test amount of marker need not be measured in absolute units, as long as the unit of measurement can be compared to a control amount and/or signal.

Preferably used are antibodies which specifically interact with the polypeptides of the present invention and not with wild type proteins or other isoforms thereof, for example. Such antibodies are directed, for example, to the unique sequence portions of the polypeptide variants of the present invention, including but not limited to bridges, heads, tails and insertions described in greater detail below. Preferred embodiments of antibodies according to the present invention are described in greater detail with regard to the section entitled “Antibodies”.

Radio-immunoassay (RIA): In one version, this method involves precipitation of the desired substrate and in the methods detailed hereinbelow, with a specific antibody and radiolabelled antibody binding protein (e.g., protein A labeled with 1125) immobilized on a precipitable carrier such as agarose beads. The number of counts in the precipitated pellet is proportional to the amount of substrate.

In an alternate version of the RIA, a labeled substrate and an unlabelled antibody binding protein are employed. A sample containing an unknown amount of substrate is added in varying amounts. The decrease in precipitated counts from the labeled substrate is proportional to the amount of substrate in the added sample.

Enzyme linked immunosorbent assay (ELISA): This method involves fixation of a sample (e.g., fixed cells or a proteinaceous solution) containing a protein substrate to a surface such as a well of a microtiter plate. A substrate specific antibody coupled to an enzyme is applied and allowed to bind to the substrate. Presence of the antibody is then detected and quantitated by a calorimetric reaction employing the enzyme coupled to the antibody. Enzymes commonly employed in this method include horseradish peroxidase and alkaline phosphatase. If well calibrated and within the linear range of response, the amount of substrate present in the sample is proportional to the amount of color produced. A substrate standard is generally employed to improve quantitative accuracy.

Western blot: This method involves separation of a substrate from other protein by means of an acrylamide gel followed by transfer of the substrate to a membrane (e.g., nylon or PVDF). Presence of the substrate is then detected by antibodies specific to the substrate, which are in turn detected by antibody binding reagents. Antibody binding reagents may be, for example, protein A, or other antibodies. Antibody binding reagents may be radiolabelled or enzyme linked as described hereinabove. Detection may be by autoradiography, calorimetric reaction or chemiluminescence. This method allows both quantitation of an amount of substrate and determination of its identity by a relative position on the membrane which is indicative of a migration distance in the acrylamide gel during electrophoresis.

Immunohistochemical analysis: This method involves detection of a substrate in situ in fixed cells by substrate specific antibodies. The substrate specific antibodies may be enzyme linked or linked to fluorophores. Detection is by microscopy and subjective evaluation. If enzyme linked antibodies are employed, a colorimetric reaction may be required.

Fluorescence activated cell sorting (FACS): This method involves detection of a substrate in situ in cells by substrate specific antibodies. The substrate specific antibodies are linked to fluorophores. Detection is by means of a cell sorting machine which reads the wavelength of light emitted from each cell as it passes through a light beam. This method may employ two or more antibodies simultaneously.

Radio-Imaging Methods

These methods include but are not limited to, positron emission tomography (PET) single photon emission computed tomography (SPECT). Both of these techniques are non-invasive, and can be used to detect and/or measure a wide variety of tissue events and/or functions, such as detecting cancerous cells for example. Unlike PET, SPECT can optionally be used with two labels simultaneously. SPECT has some other advantages as well, for example with regard to cost and the types of labels that can be used. For example, U.S. Pat. No. 6,696,686 describes the use of SPECT for detection of breast cancer, and is hereby incorporated by reference as if fully set forth herein.

Display Libraries

According to still another aspect of the present invention there is provided a display library comprising a plurality of display vehicles (such as phages, viruses or bacteria) each displaying at least 6, at least 7, at least 8, at least 9, at least 10, 10-15, 12-17, 15-20, 15-30 or 20-50 consecutive amino acids derived from the polypeptide sequences of the present invention.

Methods of constructing such display libraries are well known in the art. Such methods are described in, for example, Young A C, et al., “The three-dimensional structures of a polysaccharide binding antibody to Cryptococcus neoformans and its complex with a peptide from a phage display library: implications for the identification of peptide mimotopes” J Mol Biol 1997 Dec. 12; 274(4):622-34; Giebel L B et al. “Screening of cyclic peptide phage libraries identifies ligands that bind streptavidin with high affinities” Biochemistry 1995 Nov. 28; 34(47):15430-5; Davies E L et al., “Selection of specific phage-display antibodies using libraries derived from chicken immunoglobulin genes” J Immunol Methods 1995 Oct. 12; 186(1):125-35; Jones C RT al. “Current trends in molecular recognition and bioseparation” J Chromatogr A 1995 Jul. 14; 707(1):3-22; Deng S J et al. “Basis for selection of improved carbohydrate-binding single-chain antibodies from synthetic gene libraries” Proc Natl Acad Sci USA 1995 May 23; 92(11):4992-6; and Deng S J et al. “Selection of antibody single-chain variable fragments with improved carbohydrate binding by phage display” J Biol Chem 1994 Apr. 1; 269(13):9533-8, which are incorporated herein by reference.

The following sections relate to Candidate Marker Examples (first section) and to Experimental Data for these Marker Examples (second section). It should be noted that Table numbering is restarted within each section.

Candidate Marker Examples Section

This Section relates to Examples of sequences according to the present invention, including illustrative methods of selection thereof.

Description of the methodology undertaken to uncover the biomolecular sequences of the present invention Human ESTs and cDNAs were obtained from GenBank versions 136 (Jun. 15, 2003 ncbi.nih.gov/genbank/release.notes/gb136.release.notes); NCBI genome assembly of April 2003; RefSeq sequences from June 2003; Genbank version 139 (December 2003); Human Genome from NCBI (Build 34) (from October 2003); and RefSeq sequences from December 2003; and from the LifeSeq library of Incyte Corporation (ESTs only; Wilmington, Del., USA). With regard to GenBank sequences, the human EST sequences from the EST (GBEST) section and the human mRNA sequences from the primate (GBPRI) section were used; also the human nucleotide RefSeq mRNA sequences were used (see for example ncbi.nlm.nih.gov/Genbank/GenbankOverview.html and for a reference to the EST section, see ncbi.nlm.nih.gov/dbEST/; a general reference to dbEST, the EST database in GenBank, may be found in Boguski et al, Nat Genet. 1993 August; 4(4):332-3; all of which are hereby incorporated by reference as if fully set forth herein).

Novel splice variants were predicted using the LEADS clustering and assembly system as described in Sorek, R., Ast, G. & Graur, D. Alu-containing exons are alternatively spliced. Genome Res 12, 1060-7 (2002); U.S. Pat. No. 6,625,545; and U.S. patent application Ser. No. 10/426,002, published as US20040101876 on May 27, 2004; all of which are hereby incorporated by reference as if fully set forth herein. Briefly, the software cleans the expressed sequences from repeats, vectors and immunoglobulins. It then aligns the expressed sequences to the genome taking alternatively splicing into account and clusters overlapping expressed sequences into “clusters” that represent genes or partial genes.

These were annotated using the GeneCarta (Compugen, Tel-Aviv, Israel) platform. The GeneCarta platform includes a rich pool of annotations, sequence information (particularly of spliced sequences), chromosomal information, alignments, and additional information such as SNPs, gene ontology terms, expression profiles, functional analyses, detailed domain structures, known and predicted proteins and detailed homology reports.

A brief explanation is provided with regard to the method of selecting the candidates. However, it should noted that this explanation is provided for descriptive purposes only, and is not intended to be limiting in any way. The potential markers were identified by a computational process that was designed to find genes and/or their splice variants that are over-expressed in tumor tissues, by using databases of expressed sequences. Various parameters related to the information in the EST libraries, determined according to a manual classification process, were used to assist in locating genes and/or splice variants thereof that are over-expressed in cancerous tissues. The detailed description of the selection method is presented in Example 1 below. The cancer biomarkers selection engine and the following wet validation stages are schematically summarized in FIG. 1.

EXAMPLE 1 Identification Of differentially Expressed Gene Products—Algorithm

In order to distinguish between differentially expressed gene products and constitutively expressed genes (i.e., house keeping genes) an algorithm based on an analysis of frequencies was configured. A specific algorithm for identification of transcripts over expressed in cancer is described hereinbelow.

Dry Analysis

Library annotation—EST libraries are manually classified according to:

-   -   (i) Tissue origin     -   (ii) Biological source—Examples of frequently used biological         sources for construction of EST libraries include cancer         cell-lines; normal tissues; cancer tissues; fetal tissues; and         others such as normal cell lines and pools of normal cell-lines,         cancer cell-lines and combinations thereof. A specific         description of abbreviations used below with regard to these         tissues/cell lines etc is given above.     -   (iii) Protocol of library construction—various methods are known         in the art for library construction including normalized library         construction; non-normalized library construction; subtracted         libraries; ORESTES and others. It will be appreciated that at         times the protocol of library construction is not indicated.

The following rules were followed:

EST libraries originating from identical biological samples are considered as a single library.

EST libraries which included above-average levels of contamination, such as DNA contamination for example, were eliminated. The presence of such contamination was determined as follows. For each library, the number of unspliced ESTs that are not fully contained within other spliced sequences was counted. If the percentage of such sequences (as compared to all other sequences) was at least 4 standard deviations above the average for all libraries being analyzed, this library was tagged as being contaminated and was eliminated from further consideration in the below analysis (see also Sorek, R. & Safer, H. M. A novel algorithm for computational identification of contaminated EST libraries. Nucleic Acids Res 31, 1067-74 (2003) for further details).

Clusters (genes) having at least five sequences including at least two sequences from the tissue of interest were analyzed. Splice variants were identified by using the LEADS software package as described above.

EXAMPLE 2 Identification of Genes Over Expressed in Cancer

Two different scoring algorithms were developed.

Libraries score—candidate sequences which are supported by a number of cancer libraries, are more likely to serve as specific and effective diagnostic markers.

The basic algorithm—for each cluster the number of cancer and normal libraries contributing sequences to the cluster was counted. Fisher exact test was used to check if cancer libraries are significantly over-represented in the cluster as compared to the total number of cancer and normal libraries.

Library counting: Small libraries (e.g., less than 1000 sequences) were excluded from consideration unless they participate in the cluster. For this reason, the total number of libraries is actually adjusted for each cluster.

Clones no. score—Generally, when the number of ESTs is much higher in the cancer libraries relative to the normal libraries it might indicate actual over-expression.

The algorithm—

Clone counting: For counting EST clones each library protocol class was given a weight based on our belief of how much the protocol reflects actual expression levels:

(i) non-normalized: 1

(ii) normalized: 0.2

(iii) all other classes: 0.1

Clones number score—The total weighted number of EST clones from cancer libraries was compared to the EST clones from normal libraries. To avoid cases where one library contributes to the majority of the score, the contribution of the library that gives most clones for a given cluster was limited to 2 clones.

The score was computed as

$\frac{\frac{c + 1}{C}}{\frac{n + 1}{N}}$

where:

c—weighted number of “cancer” clones in the cluster.

C—weighted number of clones in all “cancer” libraries.

n—weighted number of “normal” clones in the cluster.

N—weighted number of clones in all “normal” libraries.

Clones number score significance—Fisher exact test was used to check if EST clones from cancer libraries are significantly over-represented in the cluster as compared to the total number of EST clones from cancer and normal libraries.

Two search approaches were used to find either general cancer-specific candidates or tumor specific candidates.

-   -   Libraries/sequences originating from tumor tissues are counted         as well as libraries originating from cancer cell-lines         (“normal” cell-lines were ignored).     -   Only libraries/sequences originating from tumor tissues are         counted

EXAMPLE 3 Identification of Tissue Specific Genes

For detection of tissue specific clusters, tissue libraries/sequences were compared to the total number of libraries/sequences in cluster. Similar statistical tools to those described in above were employed to identify tissue specific genes. Tissue abbreviations are the same as for cancerous tissues, but are indicated with the header “normal tissue”.

The algorithm—for each tested tissue T and for each tested cluster the following were examined:

1. Each cluster includes at least 2 libraries from the tissue T. At least 3 clones (weighed—as described above) from tissue T in the cluster; and

2. Clones from the tissue T are at least 40% from all the clones participating in the tested cluster

Fisher exact test P-values were computed both for library and weighted clone counts to check that the counts are statistically significant.

EXAMPLE 4 Identification of Splice Variants Over Expressed in Cancer of Clusters which are not Over Expressed in Cancer

Cancer-specific splice variants containing a unique region were identified.

Identification of unique sequence regions in splice variants

A Region is defined as a group of adjacent exons that always appear or do not appear together in each splice variant.

A “segment” (sometimes referred also as “seg” or “node”) is defined as the shortest contiguous transcribed region without known splicing inside.

Only reliable ESTs were considered for region and segment analysis. An EST was defined as unreliable if:

(i) Unspliced;

(ii) Not covered by RNA;

(iii) Not covered by spliced ESTs; and

(iv) Alignment to the genome ends in proximity of long poly-A stretch or starts in proximity of long poly-T stretch.

Only reliable regions were selected for further scoring. Unique sequence regions were considered reliable if:

(i) Aligned to the genome; and

(ii) Regions supported by more than 2 ESTs.

The algorithm

Each unique sequence region divides the set of transcripts into 2 groups:

(i) Transcripts containing this region (group TA).

(ii) Transcripts not containing this region (group TB).

The set of EST clones of every cluster is divided into 3 groups:

(i) Supporting (originating from) transcripts of group TA (SI).

(ii) Supporting transcripts of group TB (S2).

(iii) Supporting transcripts from both groups (S3).

Library and clones number scores described above were given to SI group.

Fisher Exact Test P-values were used to check if:

S1 is significantly enriched by cancer EST clones compared to S2; and

S1 is significantly enriched by cancer EST clones compared to cluster background (S1+S2+S3).

Identification of unique sequence regions and division of the group of transcripts accordingly is illustrated in FIG. 2. Each of these unique sequence regions corresponds to a segment, also termed herein a “node”.

Region 1: common to all transcripts, thus it is preferably not considered for determining differential expression between variants; Region 2: specific to Transcript 1; Region 3: specific to Transcripts 2+3; Region 4: specific to Transcript 3; Region 5: specific to Transcripts 1 and 2; Region 6: specific to Transcript 1.

EXAMPLE 5 Identification of Cancer Specific Splice Variants of Genes Over Expressed in Cancer

A search for EST supported (no mRNA) regions for genes of:

(i) known cancer markers

(ii) Genes shown to be over-expressed in cancer in published micro-array experiments.

Reliable EST supported-regions were defined as supported by minimum of one of the following:

(i) 3 spliced ESTs; or

(ii) 2 spliced ESTs from 2 libraries;

(iii) 10 unspliced ESTs from 2 libraries, or

(iv) 3 libraries.

Actual Marker Examples

The following examples relate to specific actual marker examples. It should be noted that Table numbering is restarted within each example related to a particular Cluster, as indicated by the titles below.

EXPERIMENTAL EXAMPLES SECTION

This Section relates to Examples describing experiments involving these sequences, and illustrative, non-limiting examples of methods, assays and uses thereof. The materials and experimental procedures are explained first, as all experiments used them as a basis for the work that was performed.

The markers of the present invention were tested with regard to their expression in various cancerous and non-cancerous tissue samples. A description of the samples used in the panel is provided in Table 1 below. A description of the samples used in the normal tissue panel is provided in Table 2 below. Tests were then performed as described in the “Materials and Experimental Procedures” section below.

TABLE 1 Tissue samples in testing panel Sample Lot name number Source Tissue Pathology Grade gender/age 2-A-Pap ILS- ABS ovary Papillary 2 53/F Adeno G2 1408 adenocarcinoma 3-A-Pap ILS- ABS ovary Papillary 2 52/F Adeno G2 1431 adenocarcinoma 4-A-Pap ILS- ABS ovary Papillary 2 50/F CystAdeno 7286 cystadenocarcinoma G2 1-A-Pap ILS- ABS ovary Papillary 3 73/F Adeno G3 1406 adenocarcinoma 14-B-Adeno A501111 BioChain ovary Adenocarcinoma 2 41/F G2 5-G-Adeno 99-12- GOG ovary Adenocarcinoma 3 46/F G3 G432 (Stage3C) 6-A-Adeno G3 A0106 ABS ovary adenocarcinoma 3 51/F 7-A-Adeno G3 IND- ABS ovary adenocarcinoma 3 59/F 00375 8-B-Adeno G3 A501113 BioChain ovary adenocarcinoma 3 60/F 9-G-Adeno 99-06- GOG ovary Adenocarcinoma 3 84/F G3 G901 (maybe serous) 10-B-Adeno A407069 Biochain ovary Adenocarcinoma 3 60/F G3 11-B-Adeno A407068 Biochain ovary Adenocarcinoma 3 49/F G3 12-B-Adeno A406023 Biochain ovary Adenocarcinoma 3 45/F G3 13-G-Adeno 94-05- GOG right Metastasis 3 67/F G3 7603 ovary adenocarcinoma 15-B-Adeno A407065 BioChain ovary Carcinoma 3 27/F G3 16-Ct-Adeno 1090387 Clontech ovary Carcinoma NOS F 22-A-Muc A0139 ABS ovary Mucinous 2 72/F CystAde G2 cystadenocarcinoma (Stage1C) 21-G-Muc 95-10- GOG ovary Mucinous 2-3 44/F CystAde G2-3 G020 cystadenocarcinoma (Stage2) 23-A-Muc VNM- ABS ovary Mucinous 3 45/F CystAde G3 00187 cystadenocarcinoma with low malignant 17-B-Muc A504084 BioChain ovary Mucinous 3 51/F Adeno G3 adenocarcinoma 18-B-Muc A504083 BioChain ovary Mucinous 3 45/F Adeno G3 adenocarcinoma 19-B-Muc A504085 BioChain ovary Mucinous 34/F Adeno G3 adenocarcinoma 20-A-Pap USA- ABS ovary Papillary mucinous 45/F Muc CystAde 00273 cystadenocarcinoma 33-B-Pap A503175 BioChain ovary Serous papillary 1 41/F Sero CystAde cystadenocarcinoma G1 25-A-Pap N0021 ABS ovary Papillary serous 3 55/F Sero Adeno adenocarcinoma G3 (StageT3CN1MX) 24-G-Pap 2001- GOG ovary Papillary serous 3 68/F Sero Adeno 07- adenocarcinoma G3 G801 30-G-Pap 2001- GOG ovary Papillary serous 3 72/F Sero Adeno 08- carcinoma (Stage1C) G3 G011 70-G-Pap 95-08- GOG ovary Papillary serous 3 F Sero Adeno G069 adenocarcinoma G3 31-B-Pap A503176 BioChain ovary Serous papillary 3 52/F Sero CystAde cystadenocarcinoma G3 32-G-Pap 93-09- GOG ovary Serous papillary 3 F Sero CystAde 4901 cystadenocarcinoma G3 66-G-Pap 2000- GOG ovary Papillary serous F Sero Adeno 01- carcinoma (metastais G3 SIV G413 of primary peritoneum) (Stage4) 29-G-Sero 2001- GOG right Serous 3 50/F Adeno G3 12- ovary adenocarcinoma G035 (Stage3A) 41-G-Mix 98-03- GOG ovary Mixed epithelial 2 38 Sero/Muc/Endo G803 cystadenocarcinoma G2 with mucinous, endometriold, squamous and papillary serous (Stage2) 40-G-Mix 95-11- GOG ovary, endometrium Papillary serous and 2 49/F Sero/Endo G2 G006 endometrioid, cystadenocarcinoma (Stage3C) 37-G-Mix 2002- GOG ovary Mixed serous and 3 56/F Sero/Endo G3 05- endometrioid G513 adenocarcinoma 38-G-Mix 2002- GOG ovary Mixed serous and 3 64/F Sero/Endo G3 05- endometrioid G509 adenocarcinoma of mullerian (Stage3C) 39--G-Mix 2001- GOG ovary Mixed serous and 3 F Sero/Endo G3 12- endometrioid G037 adenocarcinoma 36-G-Endo 2000- GOG ovary Endometrial 1-2 69/F Adeno G1-2 09- adenocarcinoma G621 35-G-Endo 94-08- GOG right Endometrioid 2 39/F Adeno G2 7604 ovary adenocarcinoma 34-G-Pap 95-04- GOG ovary Papillary 3 68/F Endo Adeno 2002 endometrioid G3 adenocarcinoma G3 2002 (Stage3C) 43-G-Clear 2001- GOG ovary Clear cell 3 74/F cell Adeno G3 10- adenocarcinoma G002 44-G-Clear 2001- GOG ovary Clear cell 73/F cell Adeno 07- adenocarcinoma G084 (Stage3A) 42-G-Adeno 98-08- GOG ovary Epithelial 46/F borderline G001 adenocarcinoma of borderline malignancy 59-G-Sero 98-12- GOG ovary Serous 77/F CysAdenoFibroma G401 CysAdenoFibroma 63-G-Sero 2000- GOG ovary Serous 71/F CysAdenoFibroma 10- CysAdenoFibroma of G620 borderline malignancy 64-G-Ben 99-06- GOG ovary Bengin Serous 57/F Sero G039 CysAdenoma CysAdenoma 56-G-Ben 99-01- GOG left ovary Bengin mucinus 46/F Muc G407 cysadenoma CysAdeno 62-G-Ben 99-10- GOG ovary Bengin mucinus 32/F Muc G442 cysadenoma CysAdenoma 60-G-Muc 99-01- GOG ovary Mucinous 40/F CysAdenoma G043 Cysadenoma 61-G-Muc 99-07- GOG ovary Mucinous 63/F CysAdenoma G011 Cysadenoma 65-G- 97-11- GOG right Endometrioma 41/F Endometrioma G320 ovary 57-B- A407066 BioChain ovary Thecoma 56/F Thecoma 58-CG-Stru CG-177 Ichilov ovary Struma 58/F teratoma ovary/monodermal teratoma 50-B-N M8 A501114 BioChain ovary Normal (matched 60/F tumor A501113) 49-B-N M14 A501112 BioChain ovary Normal (matched 41/F tumor A501111) 69-G-N M24 2001- GOG ovary Normal (matched 68/F 07- tumor 2001-07-G801) G801N 67-G-N M38 2002- GOG ovary Normal (matched 64/F 05- tumor 2002-05-G509) 509N 51-G-N M41 98-03- GOG ovary Normal (matched 38/F G803N tumor 98-03-G803) 52-G-N M42 98-08- GOG ovary Normal (matched 46/F G001N tumor 98-08-G001) 68-G-N M56 99-01- GOG ovary Normal (matched 46/F G407N bengin 99-01-G407) 72-G-N M66 2000- GOG ovary Normal (matched F 01- tumor 2000-01-G413) G413N 73-G-N M59 98-12- GOG ovary Normal (matched 77/F G401N tumor 98-12-G401) 74-G-N M65 97-11- GOG ovary Normal (matched 41/F G320N tumor 97-11G320) 75-G-N M60 99-01- GOG ovary Normal (matched 40/F G043N tumor 99-01-G043) 45-B-N A503274 BioChain ovary Normal PM 41/F 46-B-N A504086 BioChain ovary Normal PM 41/F 48-B-N A504087 BioChain ovary Normal PM 51/F 47-Am-N 061P43A Ambion ovary Normal (CLOSED 16/F HEAD) 71-CG-N CG- Ichilov ovary Normal PM 49/F 188-7

TABLE 2 Tissue samples in normal panel: Lot no. Source Tissue Pathology Sex/Age  1-Am-Colon (C71) 071P10B Ambion Colon PM F/43  2-B-Colon (C69) A411078 Biochain Colon PM-Pool of 10 M&F  3-Cl-Colon (C70) 1110101 Clontech Colon PM-Pool of 3 M&F  4-Am-Small Intestine 091P0201A Ambion Small Intestine PM M/75  5-B-Small Intestine A501158 Biochain Small Intestine PM M/63  6-B-Rectum A605138 Biochain Rectum PM M/25  7-B-Rectum A610297 Biochain Rectum PM M/24  8-B-Rectum A610298 Biochain Rectum PM M/27  9-Am-Stomach 110P04A Ambion Stomach PM M/16 10-B-Stomach A501159 Biochain Stomach PM M/24 11-B-Esophagus A603814 Biochain Esophagus PM M/26 12-B-Esophagus A603813 Biochain Esophagus PM M/41 13-Am-Pancreas 071P25C Ambion Pancreas PM M/25 14-CG-Pancreas CG-255-2 Ichilov Pancreas PM M/75 15-B-Lung A409363 Biochain Lung PM F/26 16-Am-Lung (L93) 111P0103A Ambion Lung PM F/61 17-B-Lung (L92) A503204 Biochain Lung PM M/28 18-Am-Ovary (O47) 061P43A Ambion Ovary PM F/16 19-B-Ovary (O48) A504087 Biochain Ovary PM F/51 20-B-Ovary (O46) A504086 Biochain Ovary PM F/41 21-Am-Cervix 101P0101A Ambion Cervix PM F/40 22-B-Cervix A408211 Biochain Cervix PM F/36 23-B-Cervix A504089 Biochain Cervix PM-Pool of 5 M&F 24-B-Uterus A411074 Biochain Uterus PM-Pool of 10 M&F 25-B-Uterus A409248 Biochain Uterus PM F/43 26-B-Uterus A504090 Biochain Uterus PM-Pool of 5 M&F 27-B-Bladder A501157 Biochain Bladder PM M/29 28-Am-Bladder 071P02C Ambion Bladder PM M/20 29-B-Bladder A504088 Biochain Bladder PM-Pool of 5 M&F 30-Am-Placenta 021P33A Ambion Placenta PB F/33 31-B-Placenta A410165 Biochain Placenta PB F/26 32-B-Placenta A411073 Biochain Placenta PB-Pool of 5 M&F 33-B-Breast (B59) A607155 Biochain Breast PM F/36 34-Am-Breast (B63) 26486 Ambion Breast PM F/43 35-Am-Breast (B64) 23036 Ambion Breast PM F/57 36-Cl-Prostate (P53) 1070317 Clontech Prostate PB-Pool of 47 M&F 37-Am-Prostate (P42) 061P04A Ambion Prostate PM M/47 38-Am-Prostate (P59) 25955 Ambion Prostate PM M/62 39-Am-Testis 111P0104A Ambion Testis PM M/25 40-B-Testis A411147 Biochain Testis PM M/74 41-Cl-Testis 1110320 Clontech Testis PB-Pool of 45 M&F 42-CG-Adrenal CG-184-10 Ichilov Adrenal PM F/81 43-B-Adrenal A610374 Biochain Adrenal PM F/83 44-B-Heart A411077 Biochain Heart PB-Pool of 5 M&F 45-CG-Heart CG-255-9 Ichilov Heart PM M/75 46-CG-Heart CG-227-1 Ichilov Heart PM F/36 47-Am-Liver 081P0101A Ambion Liver PM M/64 48-CG-Liver CG-93-3 Ichilov Liver PM F/19 49-CG-Liver CG-124-4 Ichilov Liver PM F/34 50-Cl-BM 1110932 Clontech Bone Marrow PM-Pool of 8 M&F 51-CGEN-Blood WBC#5 CGEN Blood M 52-CGEN-Blood WBC#4 CGEN Blood M 53-CGEN-Blood WBC#3 CGEN Blood M 54-CG-Spleen CG-267 Ichilov Spleen PM F/25 55-CG-Spleen 111P0106B Ambion Spleen PM M/25 56-CG-Spleen A409246 Biochain Spleen PM F/12 56-CG-Thymus CG-98-7 Ichilov Thymus PM F/28 58-Am-Thymus 101P0101A Ambion Thymus PM M/14 59-B-Thymus A409278 Biochain Thymus PM M/28 60-B-Thyroid A610287 Biochain Thyroid PM M/27 61-B-Thyroid A610286 Biochain Thyroid PM M/24 62-CG-Thyroid CG-119-2 Ichilov Thyroid PM F/66 63-Cl-Salivary Gland 1070319 Clontech Salivary Gland PM-Pool of 24 M&F 64-Am-Kidney 111P0101B Ambion Kidney PM-Pool of 14 M&F 65-Cl-Kidney 1110970 Clontech Kidney PM-Pool of 14 M&F 66-B-Kidney A411080 Biochain Kidney PM-Pool of 5 M&F 67-CG-Cerebellum CG-183-5 Ichilov Cerebellum PM M/74 68-CG-Cerebellum CG-212-5 Ichilov Cerebellum PM M/54 69-B-Brain A411322 Biochain Brain PM M/28 70-Cl-Brain 1120022 Clontech Brain PM-Pool of 2 M&F 71-B-Brain A411079 Biochain Brain PM-Pool of 2 M&F 72-CG-Brain CG-151-1 Ichilov Brain PM F/86 73-Am-Skeletal Muscle 101P013A Ambion Skeletal Muscle PM F/28 74-Cl-Skeletal Muscle 1061038 Clontech Skeletal Muscle PM-Pool of 2 M&F

Materials and Experimental Procedures

RNA preparation—RNA was obtained from Clontech (Franklin Lakes, N.J. USA 07417, clontech.com), BioChain Inst. Inc. (Hayward, Calif. 94545 USA biochain.com), ABS (Wilmington, Del. 19801, USA, absbioreagents.com) or Ambion (Austin, Tex. 78744 USA, ambion.com). Alternatively, RNA was generated from tissue samples using TRI-Reagent (Molecular Research Center), according to Manufacturer's instructions. Tissue and RNA samples were obtained from patients or from postmortem. Total RNA samples were treated with DNaseI (Ambion) and purified using RNeasy columns (Qiagen).

RT PCR—Purified RNA (1 μg) was mixed with 150 ng Random Hexamer primers (Invitrogen) and 500 μM dNTP in a total volume of 15.6 μl. The mixture was incubated for 5 min at 65° C. and then quickly chilled on ice. Thereafter, 5 μl of 5× SuperscriptII first strand buffer (Invitrogen), 2.4 μl 0.1M DTT and 40 units RNasin (Promega) were added, and the mixture was incubated for 10 min at 25° C., followed by further incubation at 42° C. for 2 min. Then, 1 μl (200 units) of SuperscriptII (Invitrogen) was added and the reaction (final volume of 25 μl) was incubated for 50 min at 42° C. and then inactivated at 70° C. for 15 min. The resulting cDNA was diluted 1:20 in TE buffer (10 mM Tris pH=8, 1 mM EDTA pH=8).

Real-Time RT-PCR analysis—cDNA (511), prepared as described above, was used as a template in Real-Time PCR reactions using the SYBR Green I assay (PE Applied Biosystem) with specific primers and UNG Enzyme (Eurogentech or ABI or Roche). The amplification was effected as follows: 50° C. for 2 min, 95° C. for 10 min, and then 40 cycles of 95° C. for 15 sec, followed by 60° C. for 1 min. Detection was performed by using the PE Applied Biosystem SDS 7000. The cycle in which the reactions achieved a threshold level (Ct) of fluorescence was registered and was used to calculate the relative transcript quantity in the RT reactions. The relative quantity was calculated using the equation Q=efficiencŷ^(−Ct). The efficiency of the PCR reaction was calculated from a standard curve, created by using serial dilutions of several reverse transcription (RT) reactions. To minimize inherent differences in the RT reaction, the resulting relative quantities were normalized to the geometric mean of the relative quantities of several housekeeping (HSKP) genes. Schematic summary of quantitative real-time PCR analysis is presented in FIG. 3. As shown, the x-axis shows the cycle number. The C_(T)=Threshold Cycle point, which is the cycle that the amplification curve crosses the fluorescence threshold that was set in the experiment. This point is a calculated cycle number in which PCR products signal is above the background level (passive dye ROX) and still in the Geometric/Exponential phase (as shown, once the level of fluorescence crosses the measurement threshold, it has a geometrically increasing phase, during which measurements are most accurate, followed by a linear phase and a plateau phase; for quantitative measurements, the latter two phases do not provide accurate measurements). The y-axis shows the normalized reporter fluorescence. It should be noted that this type of analysis provides relative quantification.

The sequences of the housekeeping genes measured in all the examples on ovarian cancerpanel were as follows:

SDHA (SEQ ID NO:1032) (GenBank Accession No. NM_004168, (SEQ ID NO:1032)) SDHA Forward primer (SEQ ID NO:1033): TGGGAACAAGAGGGCATCTG SDHA Reverse primer (SEQ ID NO:1034): CCACCACTGCATCAAATTCATG SDHA-amplicon, (SEQ ID NO:1035): TGGGAACAAGAGGGCATCTGCTAAAGTTTCAGATTCCATTTCTGCTCAGT ATCCAGTAGTGGATCATGAATTTGATGCAGTGGTGG PBGD (SEQ ID NO:1036) (GenBank Accession No. BC019323), PBGD Forward primer (SEQ ID NO:1037): TGAGAGTGATTCGCGTGGG PBGD Reverse primer (SEQ ID NO:1038): CCAGGGTACGAGGCTTTCAAT PBGD-amplicon (SEQ ID NO:1039): TGAGAGTGATTCGCGTGGGTACCCGCAAGAGCCAGCTTGCTCGCATACAG ACGGACAGTGTGGTGGCAACATTGAAAGCCTCGTACCCTGG HPRT1 (SEQ ID NO:1040) (GenBank Accession No. NM_000194), HPRT1 Forward primer (SEQ ID NO:1041): TGACACTGGCAAAACAATGCA HPRT1 Reverse primer (SEQ ID NO:1042): GGTCCTTTTCACCAGCAAGCT HPRT1-amplicon (SEQ ID NO:1043): TGACACTGGCAAAACAATGCAGACTTTGCTTTCCTTGGTCAGGCAGTATA ATCCAAAGATGGTCAAGGTCGCAAGCTTGCTGGTGAAAAGGACC GAPDH (SEQ ID NO:1044) (GenBank Accession No. BC026907) GAPDH Forward primer (SEQ ID NO:1045): TGCACCACCAACTGCTTAGC GAPDH Reverse primer (SEQ ID NO:1046): CCATCACGCCACAGTTTCC GAPDH-amplicon (SEQ ID NO:1047): TGCACCACCAACTGCTTAGCACCCCTGGCCAAGGTCATCCATGACAACTT TGGTATCGTGGAAGGACTCATGACCACAGTCCATGCCATCACTGCCACCC AGAAGACTGTGGATGG

The sequences of the housekeeping genes measured in all the examples on normal tissue samples panel were as follows:

RPL19 (SEQ ID NO:1048) (GenBank Accession No. NM_000981), RPL19 Forward primer (SEQ ID NO:1049): TGGCAAGAAGAAGGTCTGGTTAG RPL19 Reverse primer (SEQ ID NO:1050): TGATCAGCCCATCTTTGATGAG RPL19 amplicon (SEQ ID NO:1051): TGGCAAGAAGAAGGTCTGGTTAGACCCCAATGAGACCAATGAAATCGCCA ATGCCAACTCCCGTCAGCAGATCCGGAAGCTCATCAAAGATGGGCTGATC A TATA box (SEQ ID NO:1052) (GenBank Accession No. NM_003194), TATA box Forward primer (SEQ ID NO:1053): CGGTTTGCTGCGGTAATCAT TATA box Reverse primer (SEQ ID NO:1054): TTTCTTGCTGCCAGTCTGGAC TATA box amplicon (SEQ ID NO:1055): CGGTTTGCTGCGGTAATCATGAGGATAAGAGAGCCACGAACCACGGCACT GATTTTCAGTTCTGGGAAAATGGTGTGCACAGGAGCCAAGAGTGAAGAAC AGTCCAGACTGGCAGCAAGAAA Ubiquitin (SEQ ID NO: 1056) (GenBank Accession No. BC000449) Ubiquitin Forward primer (SEQ ID NO:1057): ATTTGGGTCGCGGTTCTTG Ubiquitin Reverse primer (SEQ ID NO:1058): TGCCTTGACATTCTCGATGGT Ubiquitin C amplicon (SEQ ID NO:1059): ATTTGGGTCGCGGTTCTTGTTTGTGGATCGCTGTGATCGTCACTTGACAA TGCAGATCTTCGTGAAGACTCTGACTGGTAAGACCATCACCCTCGAGGTT GAGCCCAGTGACACCATCGAGAATGTCAAGGCA SDHA (GenBank Accession No. NM_004168, (SEQ ID NO:1032)) SDHA Forward primer (SEQ ID NO:1033): TGGGAACAAGAGGGCATCTG SDHA Reverse primer (SEQ ID NO:1034): CCACCACTGCATCAAATTCATG SDHA-amplicon, (SEQ ID NO:1035): TGGGAACAAGAGGGCATCTGCTAAAGTTTCAGATTCCATTTCTGCTCAGT ATCCAGTAGTGGATCATGAATTTGATGCAGTGGTGG

Oligonucleotide-Based Micro-Array Experiment Protocol-

Microarray Fabrication

Microarrays (chips) were printed by pin deposition using the MicroGrid II MGII 600 robot from BioRobotics Limited (Cambridge, UK). 50-mer oligonucleotides target sequences were designed by Compugen Ltd (Tel-Aviv, Ill.) as described by A. Shoshan et al, “Optical technologies and informatics”, Proceedings of SPIE. Vol 4266, pp. 86-95 (2001). The designed oligonucleotides were synthesized and purified by desalting with the Sigma-Genosys system (The Woodlands, Tex., US) and all of the oligonucleotides were joined to a C6 amino-modified linker at the 5′ end, or being attached directly to CodeLink slides (Cat #25-6700-01. Amersham Bioscience, Piscataway, N.J., US). The 50-mer oligonucleotides, forming the target sequences, were first suspended in Ultra-pure DDW (Cat #01-866-1A Kibbutz Beit-Haemek, Israel) to a concentration of 50 μM. Before printing the slides, the oligonucleotides were resuspended in 300 mM sodium phosphate (pH 8.5) to final concentration of 150 mM and printed at 35-40% relative humidity at 21° C.

Each slide contained a total of 9792 features in 32 subarrays. Of these features, 4224 features were sequences of interest according to the present invention and negative controls that were printed in duplicate. An additional 288 features (96 target sequences printed in triplicate) contained housekeeping genes from Human Evaluation Library2, Compugen Ltd, Israel. Another 384 features are E. coli spikes 1-6, which are oligos to E-Coli genes which are commercially available in the Array Control product (Array control-sense oligo spots, Ambion Inc. Austin, Tex. Cat #1781, Lot #112K06).

Post-Coupling Processing of Printed Slides

After the spotting of the oligonucleotides to the glass (CodeLink) slides, the slides were incubated for 24 hours in a sealed saturated NaCl humidification chamber (relative humidity 70-75%).

Slides were treated for blocking of the residual reactive groups by incubating them in blocking solution at 50° C. for 15 minutes (10 ml/slide of buffer containing 0.1M Tris, 50 mM ethanolamine, 0.1% SDS). The slides were then rinsed twice with Ultra-pure DDW (double distilled water). The slides were then washed with wash solution (10 ml/slide. 4×SSC, 0.1% SDS)) at 50° C. for 30 minutes on the shaker. The slides were then rinsed twice with Ultra-pure DDW, followed by drying by centrifugation for 3 minutes at 800 rpm.

Next, in order to assist in automatic operation of the hybridization protocol, the slides were treated with Ventana Discovery hybridization station barcode adhesives. The printed slides were loaded on a Bio-Optica (Milan, Italy) hematology staining device and were incubated for 10 minutes in 50 ml of 3-Aminopropyl Triethoxysilane (Sigma A3648 lot #122K589). Excess fluid was dried and slides were then incubated for three hours in 20 mm/Hg in a dark vacuum desiccator (Pelco 2251, Ted Pella, Inc. Redding Calif.).

The following protocol was then followed with the Genisphere 900-RP (random primer), with mini elute columns on the Ventana Discovery HybStation™, to perform the microarray experiments. Briefly, the protocol was performed as described with regard to the instructions and information provided with the device itself. The protocol included cDNA synthesis and labeling. cDNA concentration was measured with the TBS-380 (Turner Biosystems. Sunnyvale, Calif.) PicoFlour, which is used with the OliGreen ssDNA Quantitation reagent and kit.

Hybridization was performed with the Ventana Hybridization device, according to the provided protocols (Discovery Hybridization Station Tuscon Ariz.).

The slides were then scanned with GenePix 4000B dual laser scanner from Axon Instruments Inc, and analyzed by GenePix Pro 5.0 software.

Schematic summary of the oligonucleotide based microarray fabrication and the experimental flow is presented in FIGS. 4 and 5.

Briefly, as shown in FIG. 4, DNA oligonucleotides at 25 uM were deposited (printed) onto Amersham ‘CodeLink’ glass slides generating a well defined ‘spot’. These slides are covered with a long-chain, hydrophilic polymer chemistry that creates an active 3-D surface that covalently binds the DNA oligonucleotides 5′-end via the C6-amine modification. This binding ensures that the full length of the DNA oligonucleotides is available for hybridization to the cDNA and also allows lower background, high sensitivity and reproducibility.

FIG. 5 shows a schematic method for performing the microarray experiments. It should be noted that stages on the left-hand or right-hand side may optionally be performed in any order, including in parallel, until stage 4 (hybridization). Briefly, on the left-hand side, the target oligonucleotides are being spotted on a glass microscope slide (although optionally other materials could be used) to form a spotted slide (stage 1). On the right hand side, control sample RNA and cancer sample RNA are Cy3 and Cy5 labeled, respectively (stage 2), to form labeled probes. It should be noted that the control and cancer samples come from corresponding tissues (for example, normal prostate tissue and cancerous prostate tissue). Furthermore, the tissue from which the RNA was taken is indicated below in the specific examples of data for particular clusters, with regard to overexpression of an oligonucleotide from a “chip” (microarray), as for example “prostate” for chips in which prostate cancerous tissue and normal tissue were tested as described above. In stage 3, the probes are mixed. In stage 4, hybridization is performed to form a processed slide. In stage 5, the slide is washed and scanned to form an image file, followed by data analysis in stage 6.

Description for Cluster H61775

Cluster H61775 features 2 transcript(s) and 6 segment(s) of interest, the names for which are given in Tables 1 and 2, respectively, the sequences themselves are given at the end of the application. The selected protein variants are given in table 3.

TABLE 1 Transcripts of interest Transcript Name SEQ ID NO H61775_T21 1 (SEQ ID NO:1) H61775_T22 2 (SEQ ID NO:2)

TABLE 2 Segments of interest Segment Name SEQ ID NO H61775_node_2 3 (SEQ ID NO:3) H61775_node_4 4 (SEQ ID NO:4) H61775_node_6 5 (SEQ ID NO:5) H61775_node_8 6 (SEQ ID NO:6) H61775_node_0 7 (SEQ ID NO:7) H61775_node_5 8 (SEQ ID NO:8)

TABLE 3 Proteins of interest Protein Name SEQ ID NO H61775_P16 9 (SEQ ID NO:9) H61775_P17 10 (SEQ ID NO:10)

Cluster H61775 can be used as a diagnostic marker according to overexpression of transcripts of this cluster in cancer. Expression of such transcripts in normal tissues is also given according to the previously described methods. The term “number” in the right hand column of the table and the numbers on the y-axis of FIG. 6 refer to weighted expression of ESTs in each category, as “parts per million” (ratio of the expression of ESTs for a particular cluster to the expression of all ESTs in that category, according to parts per million).

Overall, the following results were obtained as shown with regard to the histograms in FIG. 6 and Table 4. This cluster is overexpressed (at least at a minimum level) in the following pathological conditions: brain malignant tumors and a mixture of malignant tumors from different tissues.

TABLE 4 Normal tissue distribution Name of Tissue Number bladder 0 brain 0 colon 0 epithelial 10 general 3 breast 8 muscle 0 ovary 0 pancreas 0 prostate 0 uterus 0

TABLE 5 P values and ratios for expression in cancerous tissue Name of Tissue P1 P2 SP1 R3 SP2 R4 bladder 3.1e−01 3.8e−01 3.2e−01 2.5 4.6e−01 1.9 brain 8.8e−02 6.5e−02 1 3.5 4.1e−04 5.8 colon 5.6e−01 6.4e−01 1 1.1 1 1.1 epithelial 3.0e−02 1.3e−01 2.3e−02 2.1 3.2e−01 1.2 general 1.3e−06 4.9e−05 1.0e−07 6.3 1.5e−06 4.3 breast 4.7e−01 3.7e−01 3.3e−01 2.0 4.6e−01 1.6 muscle 2.3e−01 2.9e−01 1.5e−01 6.8 3.9e−01 2.6 ovary 3.8e−01 4.2e−01 1.5e−01 2.4 2.6e−01 1.9 pancreas 3.3e−01 4.4e−01 4.2e−01 2.4 5.3e−01 1.9 prostate 7.3e−01 7.8e−01 6.7e−01 1.5 7.5e−01 1.3 uterus 1.0e−01 2.6e−01 2.9e−01 2.6 5.1e−01 1.8 As noted above, cluster H61775 features 2 transcript(s), which were listed in Table 1 above. A description of each variant protein according to the present invention is now provided.

Variant protein H61775_P16 (SEQ ID NO: 9) according to the present invention has an amino acid sequence as given at the end of the application; it is encoded by transcript(s) H61775_T21 (SEQ ID NO: 1). One or more alignments to one or more previously published protein sequences are given at the end of the application. A brief description of the relationship of the variant protein according to the present invention to each such aligned protein is as follows:

Comparison report between H61775_P16 (SEQ ID NO: 9) and Q9P2J2 (SEQ ID NO: 953) (SEQ ID NO:953):

1. An isolated chimeric polypeptide encoding for H61775_P16 (SEQ ID NO: 9), comprising a first amino acid sequence being at least 90% homologous to MVWCLGLAVLSLVISQGADGRGKPEVVSVVGRAGESVVLGCDLLPPAGRPPLHVIEWL RFGFLLPIFIQFGLYSPRIDPDYVG corresponding to amino acids 11-93 of Q9P2J2 (SEQ ID NO: 953), which also corresponds to amino acids 1-83 of H61775_P16 (SEQ ID NO: 9), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence DCGFPAFRELKRAETVSPVFFTRRCIWEDLKSTGFSPAGGGRPPGGGPRTQEDSGLPCW RSSCSVTLQV (SEQ ID NO: 1110) corresponding to amino acids 84-152 of H61775_P16 (SEQ ID NO: 9), wherein said first and second amino acid sequences are contiguous and in a sequential order.

2. An isolated polypeptide encoding for a tail of H61775_P16 (SEQ ID NO: 9), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence DCGFPAFRELKRAETVSPVFFTRRCIWEDLKSTGFSPAGGGRPPGGGPRTQEDSGLPCW RSSCSVTLQV (SEQ ID NO: 1110) in H61775_P16 (SEQ ID NO: 9).

Comparison report between H61775_P16 (SEQ ID NO: 9) and AAQ88495 (SEQ ID NO: 954) (SEQ ID NO:954):

1. An isolated chimeric polypeptide encoding for H61775_P16 (SEQ ID NO: 9), comprising a first amino acid sequence being at least 90% homologous to MVWCLGLAVLSLVISQGADGRGKPEVVSVVGRAGESVVLGCDLLPPAGRPPLHVIEWL RFGFLLPIFIQFGLYSPRIDPDYVG corresponding to amino acids 1-83 of AAQ88495 (SEQ ID NO: 954), which also corresponds to amino acids 1-83 of H61775_P16 (SEQ ID NO: 9), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence DCGFPAFRELKRAETVSPVFFTRRCIWEDLKSTGFSPAGGGRPPGGGPRTQEDSGLPCW RSSCSVTLQV (SEQ ID NO: 1110) corresponding to amino acids 84-152 of H61775_P16 (SEQ ID NO: 9), wherein said first and second amino acid sequences are contiguous and in a sequential order.

2. An isolated polypeptide encoding for a tail of H61775_P16 (SEQ ID NO: 9), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence DCGFPAFRELKRAETVSPVFFTRRCIWEDLKSTGFSPAGGGRPPGGGPRTQEDSGLPCW RSSCSVTLQV (SEQ ID NO: 1110) in H61775_P16 (SEQ ID NO: 9).

The location of the variant protein was determined according to results from a number of different software programs and analyses, including analyses from SignalP and other specialized programs. The variant protein is believed to be located as follows with regard to the cell: secreted. The protein localization is believed to be secreted because both signal-peptide prediction programs predict that this protein has a signal peptide, and neither trans-membrane region prediction program predicts that this protein has a trans-membrane region.

Variant protein H61775_P16 (SEQ ID NO: 9) also has the following non-silent SNPs (Single Nucleotide Polymorphisms) as listed in Table 6, (given according to their position(s) on the amino acid sequence, with the alternative amino acid(s) listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein H61775_P16 (SEQ ID NO: 9) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 6 Amino acid mutations SNP position(s) on amino acid Alternative sequence amino acid(s) Previously known SNP? 14 I -> T No 138 G -> R No 34 G -> E Yes 48 G -> R No 91 R -> * Yes

Variant protein H61775_P16 (SEQ ID NO: 9) is encoded by the following transcript(s): H61775_T21 (SEQ ID NO: 1), for which the sequence(s) is/are given at the end of the application. The coding portion of transcript H61775_T21 (SEQ ID NO: 1) is shown in bold; this coding portion starts at position 261 and ends at position 716. The transcript also has the following SNPs as listed in Table 7 (given according to their position on the nucleotide sequence, with the alternative nucleic acid listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein H61775_P16 (SEQ ID NO: 9) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 7 Nucleic acid SNPs SNP position on nucleotide Alternative sequence nucleic acid Previously known SNP? 117 T -> C Yes 200 T -> C No 672 G -> C No 222 T -> C Yes 301 T -> C No 361 G -> A Yes 377 G -> A No 400 -> C No 402 G -> C No 531 C -> T Yes 566 T -> C No

Variant protein H61775_P17 (SEQ ID NO: 10) according to the present invention has an amino acid sequence as given at the end of the application; it is encoded by transcript(s) H61775_T22 (SEQ ID NO: 2). One or more alignments to one or more previously published protein sequences are given at the end of the application. A brief description of the relationship of the variant protein according to the present invention to each such aligned protein is as follows:

Comparison report between H61775_P17 (SEQ ID NO: 10) and Q9P2J2 (SEQ ID NO: 953):

1. An isolated chimeric polypeptide encoding for H61775_P17 (SEQ ID NO: 10), comprising a first amino acid sequence being at least 90% homologous to MVWCLGLAVLSLVISQGADGRGKPEVVSVVGRAGESVVLGCDLLPPAGRPPLHVIEWL RFGFLLPIFIQFGLYSPRIDPDYVG corresponding to amino acids 11-93 of Q9P2J2 (SEQ ID NO: 953), which also corresponds to amino acids 1-83 of H61775_P17 (SEQ ID NO: 10).

Comparison report between H61775_P17 (SEQ ID NO: 10) and AAQ88495 (SEQ ID NO: 954):

1. An isolated chimeric polypeptide encoding for H61775_P17 (SEQ ID NO: 10), comprising a first amino acid sequence being at least 90% homologous to MVWCLGLAVLSLVISQGADGRGKPEVVSVVGRAGESVVLGCDLLPPAGRPPLHVIEWL RFGFLLPIFIQFGLYSPRIDPDYVG corresponding to amino acids 1-83 of AAQ88495 (SEQ ID NO: 954), which also corresponds to amino acids 1-83 of H61775_P17 (SEQ ID NO: 10).

The location of the variant protein was determined according to results from a number of different software programs and analyses, including analyses from SignalP and other specialized programs. The variant protein is believed to be located as follows with regard to the cell: secreted. The protein localization is believed to be secreted because both signal-peptide prediction programs predict that this protein has a signal peptide, and neither trans-membrane region prediction program predicts that this protein has a trans-membrane region.

Variant protein H61775_P17 (SEQ ID NO: 10) also has the following non-silent SNPs (Single Nucleotide Polymorphisms) as listed in Table 8, (given according to their position(s) on the amino acid sequence, with the alternative amino acid(s) listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein H61775_P17 (SEQ ID NO: 10) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 8 Amino acid mutations SNP position(s) on amino acid Alternative sequence amino acid(s) Previously known SNP? 14 I -> T No 34 G -> E Yes 48 G -> R No

Variant protein H61775_P17 (SEQ ID NO: 10) is encoded by the following transcript(s): H61775_T22 (SEQ ID NO: 2), for which the sequence(s) is/are given at the end of the application. The coding portion of transcript H61775_T22 (SEQ ID NO: 2) is shown in bold; this coding portion starts at position 261 and ends at position 509. The transcript also has the following SNPs as listed in Table 9 (given according to their position on the nucleotide sequence, with the alternative nucleic acid listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein H61775_P17 (SEQ ID NO: 10) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 9 Nucleic acid SNPs SNP position on nucleotide Alternative sequence nucleic acid Previously known SNP? 117 T -> C Yes 200 T -> C No 222 T -> C Yes 301 T -> C No 361 G -> A Yes 377 G -> A No 400 -> C No 402 G -> C No 596 T -> A Yes

As noted above, cluster H61775 features 6 segment(s), which were listed in Table 2 above and for which the sequence(s) are given at the end of the application. These segment(s) are portions of nucleic acid sequence(s) which are described herein separately because they are of particular interest. A description of each segment according to the present invention is now provided.

Segment cluster H61775_node_(—)2 (SEQ ID NO: 3) according to the present invention is supported by 17 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): H61775_T21 (SEQ ID NO: 1) and H61775_T22 (SEQ ID NO: 2). Table 10 below describes the starting and ending position of this segment on each transcript.

TABLE 10 Segment location on transcripts Segment Segment Transcript starting ending name position position H61775_T21 87 318 (SEQ ID NO:1) H61775_T22 87 318 (SEQ ID NO:2)

Segment cluster H61775_node_(—)4 (SEQ ID NO: 4) according to the present invention is supported by 20 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): H61775_T21 (SEQ ID NO: 1) and H61775_T22 (SEQ ID NO: 2). Table 11 below describes the starting and ending position of this segment on each transcript.

TABLE 11 Segment location on transcripts Segment Segment Transcript starting ending name position position H61775_T21 319 507 (SEQ ID NO:1) H61775_T22 319 507 (SEQ ID NO:2)

Segment cluster H61775_node_(—)6 (SEQ ID NO: 5) according to the present invention is supported by 1 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): H61775_T22 (SEQ ID NO: 2). Table 12 below describes the starting and ending position of this segment on each transcript.

TABLE 12 Segment location on transcripts Segment Segment Transcript starting ending name position position H61775_T22 515 715 (SEQ ID NO:2)

Segment cluster H61775_node_(—)8 (SEQ ID NO: 6) according to the present invention is supported by 5 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): H61775_T21 (SEQ ID NO: 1). Table 13 below describes the starting and ending position of this segment on each transcript.

TABLE 13 Segment location on transcripts Segment Segment Transcript starting ending name position position H61775_T21 508 1205 (SEQ ID NO:1)

According to an optional embodiment of the present invention, short segments related to the above cluster are also provided. These segments are up to about 120 bp in length, and so are included in a separate description.

Segment cluster H61775_node_(—)0 (SEQ ID NO: 7) according to the present invention is supported by 4 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): H61775_T21 (SEQ ID NO: 1) and H61775_T22 (SEQ ID NO: 2). Table 14 below describes the starting and ending position of this segment on each transcript.

TABLE 14 Segment location on transcripts Segment Segment Transcript starting ending name position position H61775_T21 1 86 (SEQ ID NO:1) H61775_T22 1 86 (SEQ ID NO:2)

Segment cluster H61775_node_(—)5 (SEQ ID NO: 8) according to the present invention can be found in the following transcript(s): H61775_T22 (SEQ ID NO: 2). Table 15 below describes the starting and ending position of this segment on each transcript.

TABLE 15 Segment location on transcripts Segment Segment Transcript starting ending name position position H61775_T22 508 514 (SEQ ID NO:2) Variant protein alignment to the previously known protein: Sequence name: /tmp/Psw0RJLCti/aLAXQjXh07:Q9P2J2 (SEQ ID NO: 953)

Sequence Documentation: Alignment of: H61775_P16 (SEQ ID NO: 9) x Q9P2J2 (SEQ ID NO: 953) ..

Alignment segment 1/1:

Quality: 803.00 Escore: 0 Matching length: 83 Total length: 83 Matching Percent Similarity: 100.00 Matching Percent Identity: 100.00 Total Percent Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0

Alignment:

Sequence name: /tmp/Psw0RJLCti/aLAXQjXh07:AAQ88495 (SEQ ID NO: 954) Sequence documentation: PS Alignment of: H61775_P16 (SEQ ID NO: 9) x AAQ88495 (SEQ ID NO: 954) Alignment segment 1/1:

Quality: 803.00 Escore: 0 Matching length: 83 Total length: 83 Matching Percent Similarity: 100.00 Matching Percent Identity: 100.00 Total Percent Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0

Alignment:

Sequence name: /tmp/naab8yR3GC/pSM4l2IL5o:Q9P2J2 (SEQ ID NO: 953) Sequence documentation:

Alignment of: H61775_P17 (SEQ ID NO: 10) x Q9P2J2 (SEQ ID NO: 953) ..

Quality: 803.00 Escore: 0 Matching length: 83 Total length: 83 Matching Percent Similarity: 100.00 Matching Percent Identity: 100.00 Total Percent Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0

Alignment:

Sequence name: /tmp/naab8yR3GC/pSM4l2IL5o:AAQ88495 (SEQ ID NO: 954) Sequence documentation:

Alignment of: H61775_P17 (SEQ ID NO: 10) x AAQ88495 (SEQ ID NO: 954) ..

Alignment segment 1/1:

Quality: 803.00 Escore: 0 Matching length: 83 Total length: 83 Matching Percent Similarity: 100.00 Matching Percent Identity: 100.00 Total Percent Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0

Alignment:

Expression of immunoglobulin superfamily, member 9 H61775 transcripts which are detectable by amplicon as depicted in sequence name H61775seg8 (SEQ ID NO:957) in normal and cancerous ovary tissues.

Expression of immunoglobulin superfamily, member 9 transcripts detectable by or according to H61775seg8 (SEQ ID NO:957), H61775seg8 (SEQ ID NO:957) amplicon(s) and H61775seg8F2 (SEQ ID NO:955) and H61775seg8R2 (SEQ ID NO:956) primers was measured by real time PCR. In parallel the expression of four housekeeping genes: PBGD (GenBank Accession No. BC019323, (SEQ ID NO:1036); amplicon—PBGD-amplicon, (SEQ ID NO:1039)), HPRT1 (GenBank Accession No. NM_(—)000194, (SEQ ID NO:1040); amplicon—HPRT1-amplicon, (SEQ ID NO:1043)), and SDHA (GenBank Accession No. NM_(—)004168, (SEQ ID NO:1032); amplicon—SDHA-amplicon, (SEQ ID NO:1035)), GAPDH (GenBank Accession No. BC026907, (SEQ ID NO:1044); GAPDH amplicon, (SEQ ID NO:1047)) was measured similarly. For each RT sample, the expression of the above amplicon was normalized to the geometric mean of the quantities of the housekeeping genes. The normalized quantity of each RT sample was then divided by the median of the quantities of the normal post-mortem (PM) samples (Sample Nos. 45-48, 71, Table 1, “Tissue samples in testing panel”), to obtain a value of fold up-regulation for each sample relative to median of the normal PM samples.

FIG. 7 is a histogram showing over expression of the above-indicated immunoglobulin superfamily, member 9 transcripts in cancerous ovary samples relative to the normal samples. (Values represent the average of duplicate experiments. Error bars indicate the minimal and maximal values obtained

As is evident from FIG. 7, the expression of immunoglobulin superfamily, member 9 transcripts detectable by the above amplicon(s) in cancer samples was significantly higher than in the non-cancerous samples (Sample Nos. 45-48, 71 Table 1, “Tissue samples in testing panel”) and including benign samples (samples No. 56, 62, 64). Notably an over-expression of at least 5 fold was found in 21 out of 43 adenocarcinoma samples.

Statistical analysis was applied to verify the significance of these results, as described below.

The P value for the difference in the expression levels of immunoglobulin superfamily, member 9 transcripts detectable by the above amplicon(s) in ovary cancer samples versus the normal tissue samples was determined by T test as 2.76E-4.

The above value demonstrates statistical significance of the results.

Primer pairs are also optionally and preferably encompassed within the present invention; for example, for the above experiment, the following primer pair was used as a non-limiting illustrative example only of a suitable primer pair: H61775seg8F2 (SEQ ID NO:955) forward primer; and H61775seg8R2 (SEQ ID NO:956) reverse primer.

The present invention also preferably encompasses any amplicon obtained through the use of any suitable primer pair; for example, for the above experiment, the following amplicon was obtained as a non-limiting illustrative example only of a suitable amplicon: H61775seg8 (SEQ ID NO:957)

H61775seg8F2 (SEQ ID NO:955) GAAGGCTCTTGTCACTTACTAGCCAT H61775seg8R2 (SEQ ID NO:956) TGTCACCATATTTAATCCTCCCAA Amplicon (SEQ ID NO:957) GAAGGCTCTTGTCACTTACTAGCCATGTGATTTTGGAAAGAAACTTAACA TTAATTCCTTCAGCTACAATGGAATTCTTGGGAGGATTAAATATGGTGAC A Expression of immunoglobulin superfamily, member 9H61775 transcripts which are detectable by amplicon as depicted in sequence name H61775seg8 (SEQ ID NO:957) in different normal tissues.

Expression of immunoglobulin superfamily, member 9 transcripts detectable by or according to H61775 seg8 amplicon(s) and H61775 seg8F and H61775 seg8R was measured by real time PCR. In parallel the expression of four housekeeping genes -RPL19 (GenBank Accession No. NM_(—)000981, (SEQ ID NO:1048); RPL19 amplicon, (SEQ ID NO:1051)), TATA box (GenBank Accession No. NM_(—)003194, (SEQ ID NO:1052); TATA amplicon, (SEQ ID NO:1055)), Ubiquitin (GenBank Accession No. BC000449, (SEQ ID NO:1056); amplicon—Ubiquitin-amplicon, (SEQ ID NO: 1059)) and SDHA (GenBank Accession No. NM_(—)004168, (SEQ ID NO: 1032); amplicon—SDHA-amplicon, (SEQ ID NO: 1035)) was measured similarly. For each RT sample, the expression of the above amplicon was normalized to the geometric mean of the quantities of the housekeeping genes. The normalized quantity of each RT sample was then divided by the median of the quantities of the ovary samples (Sample Nos. 18-20, Table 2 “Tissue samples in normal panel”, above), to obtain a value of relative expression of each sample relative to median of the ovary samples.

The results are described in FIG. 8, presenting the histogram showing the expression of H61775 transcripts which are detectable by amplicon as depicted in sequence name H61775seg8 (SEQ ID NO:957), in different normal tissues. Amplicon and primers are as above.

Description for Cluster HSAPHOL

Cluster HSAPHOL features 7 transcript(s) and 18 segment(s) of interest, the names for which are given in Tables 1 and 2, respectively, the sequences themselves are given at the end of the application. The selected protein variants are given in table 3.

TABLE 1 Transcripts of interest Transcript Name SEQ ID NO: HSAPHOL_T10 11 HSAPHOL_T4 12 HSAPHOL_T5 13 HSAPHOL T6 14 HSAPHOL_T7 15 HSAPHOL_T8 16 HSAPHOL_T9 17

TABLE 2 Segments of interest Segment Name SEQ ID NO: HSAPHOL_node_11 18 HSAPHOL_node_13 19 HSAPHOL_node_15 20 HSAPHOL_node_19 21 HSAPHOL_node_2 22 HSAPHOL_node_21 23 HSAPHOL_node_23 24 HSAPHOL_node_26 25 HSAPHOL_node_28 26 HSAPHOL_node_38 27 HSAPHOL_node_40 28 HSAPHOL_node_42 29 HSAPHOL_node_16 30 HSAPHOL_node_25 31 HSAPHOL_node_34 32 HSAPHOL_node_35 33 HSAPHOL_node_36 34 HSAPHOL_node_41 35

TABLE 3 Proteins of interest Protein Name SEQ ID NO: HSAPHOL_P2 37 HSAPHOL P3 38 HSAPHOL_P4 39 HSAPHOL P5 40 HSAPHOL_P6 41 HSAPHOL_P7 42 HSAPHOL_P8 43

These sequences are variants of the known protein Alkaline phosphatase, tissue-nonspecific isozyme precursor (SwissProt accession identifier PPBT_HUMAN; known also according to the synonyms EC 3.1.3.1; AP-TNAP; Liver/bone/kidney isozyme; TNSALP), SEQ ID NO: 36, referred to herein as the previously known protein.

The variant proteins according to the present invention are variant(s) of a known diagnostic marker, called Alkaline Phosphatase.

Protein Alkaline phosphatase, tissue-nonspecific isozyme precursor is known or believed to have the following function(s): THIS ISOZYME MAY PLAY A ROLE IN SKELETAL MINERALIZATION. The sequence for protein Alkaline phosphatase, tissue-nonspecific isozyme precursor is given at the end of the application, as “Alkaline phosphatase, tissue-nonspecific isozyme precursor amino acid sequence”. Known polymorphisms for this sequence are as shown in Table 4.

TABLE 4 Amino acid mutations for Known Protein SNP position(s) on amino acid sequence Comment 28 Y -> C (in hypophosphatasia; infantile; 7% of activity). /FTId = VAR_013972. 33 A -> V (in hypophosphatasia). /FTId = VAR_006147. 111 A -> T (in hypophosphatasia; odonto). /FTId = VAR_006151. 116 A -> T (in hypophosphatasia; loss of activity). /FTId = VAR_013977. 120 G -> R (in hypophosphatasia). /FTId = VAR_013978. 129 G -> R (in hypophosphatasia). /FTId = VAR_013979. 132 A -> V (in hypophosphatasia). /FTId = VAR_013146. 134 T -> N (in hypophosphatasia; 9% of activity). /FTId = VAR_011082. 136 R -> H (in hypophosphatasia; moderate; 33% of activity). /FTId = VAR_006152. 152 R -> H (in hypophosphatasia). /FTId = VAR_013980. 162 G -> V (in hypophosphatasia; severe; 1% of activity). /FTId = VAR_006153. 170 N -> D (in hypophosphatasia). /FTId = VAR_013981. 40 A -> V (in hypophosphatasia; 2% of activity). /FTId = VAR_011081. 171 H -> Y (in hypophosphatasia; severe; 2% of activity). /FTId = VAR_006154. 176 A -> T (in hypophosphatasia). /FTId = VAR_011083. 177 A -> T (in hypophosphatasia; adult type). /FTId = VAR_006155. 179 A -> T (in hypophosphatasia). /FTId = VAR_006156. 181 S -> L (in hypophosphatasia; 1% OF activity). /FTId = VAR_013982. 184 R -> W (in hypophosphatasia; loss of activity). /FTId = VAR_013983. 191 E -> G (in hypophosphatasia; odonto). /FTId = VAR_006157. 191 E -> K (in hypophosphatasia; moderate; frequent mutation in European countries). /FTId = VAR_006158. 201 C -> Y (in hypophosphatasia). /FTId = VAR_006159. 207 Q -> P (in hypophosphatasia). /FTId = VAR_006160. 51 A -> V (in hypophosphatasia). /FTId = VAR_013973. 211 N -> D (in hypophosphatasia). /FTId = VAR_013984. 220 G -> V (in hypophosphatasia; odonto). /FTId = VAR_013985. 223 R -> W (in hypophosphatasia; 3% of activity). /FTId = VAR_013986. 224 K -> E (in hypophosphatasia; infantile; partial loss of activity). /FTId = VAR_011084. 235 E -> G (in hypophosphatasia). /FTId = VAR_013987. 246 R -> S (in hypophosphatasia; 4% of activity). /FTId = VAR_011085. 249 G -> V (in hypophosphatasia; partial loss of activity). /FTId = VAR_013988. 263 H -> Y (common polymorphism). /FTId = VAR_006161. 289 L -> F (in hypophosphatasia). /FTId = VAR_006162. 291 E -> K (in hypophosphatasia; moderate; 8% of activity). /FTId = VAR_013989. 62 M -> L (in hypophosphatasia; moderate; 27% of activity). /FTId = VAR_006148. 294 D -> A (in hypophosphatasia). /FTId = VAR_006163. 294 D -> Y (in hypophosphatasia). /FTId = VAR_013990. 306 D -> V (in hypophosphatasia). /FTId = VAR_006164. 326 G -> R (in hypophosphatasia; in a patient carrying also lys- 291). /FTId = VAR_013991. 327 F -> G (in hypophosphatasia; requires 2 nucleotides substitutions). /FTId = VAR_013992. 327 F -> L (in hypophosphatasia; childhood). /FTId = VAR_006165. 334 G -> D (in hypophosphatasia). /FTId = VAR_006166. 348 A -> T (in hypophosphatasia). /FTId = VAR_011086. 378 D -> V (in hypophosphatasia; loss of activity). /FTId = VAR_006167. 381 H -> R (in hypophosphatasia). /FTId = VAR_011087. 63 G -> V (in hypophosphatasia; loss of activity). /FTId = VAR_013974. 382 V -> I (in hypophosphatasia). /FTId = VAR_006168. 391 R -> C (in hypophosphatasia; moderate; 10% of activity). /FTId = VAR_013993. 399 A -> S (in hypophosphatasia). /FTId = VAR_013994. 406 D -> G (in hypophosphatasia; 15% of activity). /FTId = VAR_011088. 423 V -> A (in hypophosphatasia; 16% of activity). /FTId = VAR_013995. 426 G -> C (in hypophosphatasia; infantile; partial loss of activity). /FTId = VAR_011089. 436 Y -> H (in hypophosphatasia). /FTId = VAR_006169. 445 S -> P (in hypophosphatasia; severe; 2% of activity). /FTId = VAR_013996. 450 R -> C (in hypophosphatasia; severe; 4% of activity). /FTId = VAR_013997. 450 R -> H (in hypophosphatasia). /FTId = VAR_011090. 71 R -> C (in hypophosphatasia). /FTId = VAR_006149. 456 G -> R (in hypophosphatasia; loss of activity). /FTId = VAR_011091. 459 V -> M (in hypophosphatasia; infantile). /FTId = VAR_013998. 473 G -> S (in hypophosphatasia). /FTId = VAR_013999. 476 E -> K (in hypophosphatasia). /FTId = VAR_006170. 478 N -> I (in hypophosphatasia; 9% of activity). /FTId = VAR_011092. 489 C -> S (in hypophosphatasia; 9% of activity). /FTId = VAR_011093. 490 I -> F (in hypophosphatasia; odonto; partial loss of activity). /FTId = VAR_014000. 491 G -> R (in hypophosphatasia). /FTId = VAR_014001. 522 V -> A. /FTId = VAR_011094. 29 W -> A 71 R -> H (in hypophosphatasia). /FTId = VAR_013975. 104 N -> K 71 R -> P (in hypophosphatasia). /FTId = VAR_006150. 75 G -> S (in hypophosphatasia; severe; 3.5% of activity). /FTId = VAR_013976.

Protein Alkaline phosphatase, tissue-nonspecific isozyme precursor localization is believed to be attached to the membrane by a GPI-anchor.

The following GO Annotation(s) apply to the previously known protein. The following annotation(s) were found: skeletal development; ossification; metabolism, which are annotation(s) related to Biological Process; magnesium binding; alkaline phosphatase; hydrolase, which are annotation(s) related to Molecular Function; and integral membrane protein, which are annotation(s) related to Cellular Component.

The GO assignment relies on information from one or more of the SwissProt/TremBl Protein knowledgebase, available from <http://www.expasy.ch/sprot/>; or Locuslink, available from <http://www.ncbi.nlm.nih.gov/projects/LocusLinkl>.

As noted above, cluster HSAPHOL features 7 transcript(s), which were listed in Table 1 above. These transcript(s) encode for protein(s) which are variant(s) of protein Alkaline phosphatase, tissue-nonspecific isozyme precursor. A description of each variant protein according to the present invention is now provided.

Variant protein HSAPHOL_P2 (SEQ ID NO: 37) according to the present invention has an amino acid sequence as given at the end of the application; it is encoded by transcript(s) HSAPHOL_T4 (SEQ ID NO: 12). An alignment is given to the known protein (Alkaline phosphatase, tissue-nonspecific isozyme precursor) at the end of the application. One or more alignments to one or more previously published protein sequences are given at the end of the application. A brief description of the relationship of the variant protein according to the present invention to each such aligned protein is as follows:

Comparison report between HSAPHOL_P2 (SEQ ID NO: 37) and AAH21289 (SEQ ID NO: 36):

1. An isolated chimeric polypeptide encoding for HSAPHOL_P2 (SEQ ID NO: 37), comprising a first amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence PHSGPAAAFIRRRGWWPGPRCA (SEQ ID NO: 1111) corresponding to amino acids 1-22 of HSAPHOL_P2 (SEQ ID NO: 37), second amino acid sequence being at least 90% homologous to PATPRPLSWLRAPTRLCLDGPSPVLCA corresponding to amino acids 1-27 of AAH21289, which also corresponds to amino acids 23-49 of HSAPHOL_P2 (SEQ ID NO: 37), and a third amino acid sequence being at least 90% homologous to EKEKDPKYWRDQAQETLKYALELQKLNTNVAKNVIMFLGDGMGVSTVTAARILKGQL HHNPGEETRLEMDKFPFVALSKTYNTNAQVPDSAGTATAYLCGVKANEGTVGVSAAT ERSRCNTTQGNEVTSILRWAKDAGKSVGIVTTTRVNHATPSAAYAHSADRDWYSDNE MPPEALSQGCKDIAYQLMHNIRDIDVIMGGGRKYMYPKNKTDVEYESDEKARGTRLD GLDLVDTWKSFKPRYKHSHFIWNRTELLTLDPHNVDYLLGLFEPGDMQYELNRNNVT DPSLSEMVVVAIQILRKNPKGFFLLVEGGRIDHGHHEGKAKQALHEAVEMDRAIGQAG SLTSSEDTLTVVTADHSHVFTFGGYTPRGNSIFGLAPMLSDTDKKPFTAILYGNGPGYK VVGGERENVSMVDYAHNNYQAQSAVPLRHETHGGEDVAVFSKGPMAHLLHGVHEQN YVPHVMAYAACIGANLGHCAPASSAGSLAAGPLLLALALYPLSVLF corresponding to amino acids 83-586 of AAH21289, which also corresponds to amino acids 50-553 of HSAPHOL_P2 (SEQ ID NO: 37), wherein said first, second and third amino acid sequences are contiguous and in a sequential order.

2. An isolated polypeptide encoding for a head of HSAPHOL_P2 (SEQ ID NO: 37), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence PHSGPAAAFIRRRGWWPGPRCA (SEQ ID NO: 1111) of HSAPHOL_P2 (SEQ ID NO: 37).

3. An isolated chimeric polypeptide encoding for an edge portion of HSAPHOL_P2 (SEQ ID NO: 37), comprising a polypeptide having a length “n”, wherein n is at least about 10 amino acids in length, optionally at least about 20 amino acids in length, preferably at least about 30 amino acids in length, more preferably at least about 40 amino acids in length and most preferably at least about 50 amino acids in length, wherein at least two amino acids comprise AE, having a structure as follows: a sequence starting from any of amino acid numbers 49-x to 49; and ending at any of amino acid numbers 50+((n−2)−x), in which x varies from 0 to n−2.

Comparison report between HSAPHOL_P2 (SEQ ID NO: 37) and PPBT_HUMAN:

1. An isolated chimeric polypeptide encoding for HSAPHOL_P2 (SEQ ID NO: 37), comprising a first amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence PHSGPAAAFIRRRGWWPGPRCAPATPRPLSWLRAPTRLCLDGPSPVLCA corresponding to amino acids 1-49 of HSAPHOL_P2 (SEQ ID NO: 37), second amino acid sequence being at least 90% homologous to EKEKDPKYWRDQAQETLKYALELQKLNTNVAKNVIMFLGDGMGVSTVTAARILKGQL HHNPGEETRLEMDKFPFVALSKTYNTNAQVPDSAGTATAYLCGVKANEGTVGVSAAT ERSRCNTTQGNEVTSILRWAKDAGKSVGIVTTTRVNHATPSAAYAHSADRDWYSDNE MPPEALSQGCKDIAYQLMHNIRDIDVIMGGGRKYMYPKNKTDVEYESDEKARGTRLD GLDLVDTWKSFKPRYKHSHFIWNRTELLTLDPHNVDYLLGLFEPGDMQYELNRNNVT DPSLSEMVVVAIQILRKNPKGFFLLVEGGRIDHGHHEGKAKQALHEAVEMDRAIGQAG SLTSSEDTLTVVTADHSHVFTFGGYTPRGNSIFGLAPMLSDTDKKPFTAILYGNGPGYK VVGGERENVSMVDYAHNNYQAQSAVPLRHETHGGEDVAVFSKGPMAHLLHGVHEQN YVPHVMAYAACIGANLGHCAPASSAGSLAAGPLLLALALYPLSVLF corresponding to amino acids 21-524 of PPBT_HUMAN, which also corresponds to amino acids 50-553 of HSAPHOL_P2 (SEQ ID NO: 37), wherein said first, second and third amino acid sequences are contiguous and in a sequential order.

2. An isolated polypeptide encoding for a head of HSAPHOL_P2 (SEQ ID NO: 37), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence PHSGPAAAFIRRRGWWPGPRCAPATPRPLSWLRAPTRLCLDGPSPVLCA of HSAPHOL_P2 (SEQ ID NO: 37).

3. An isolated chimeric polypeptide encoding for an edge portion of HSAPHOL_P2 (SEQ ID NO: 37), comprising a polypeptide having a length “n”, wherein n is at least about 10 amino acids in length, optionally at least about 20 amino acids in length, preferably at least about 30 amino acids in length, more preferably at least about 40 amino acids in length and most preferably at least about 50 amino acids in length, wherein at least two amino acids comprise AE, having a structure as follows: a sequence starting from any of amino acid numbers 49-x to 49; and ending at any of amino acid numbers 50+((n−2)−x), in which x varies from 0 to n−2.

The location of the variant protein was determined according to results from a number of different software programs and analyses, including analyses from SignalP and other specialized programs. The variant protein is believed to be located as follows with regard to the cell: membrane. The protein localization is believed to be membrane because although it is a partial protein, because both trans-membrane region prediction programs predict that this protein has a trans-membrane region, and similarity to known proteins suggests a GPI anchor. Variant protein HSAPHOL_P2 (SEQ ID NO: 37) also has the following non-silent SNPs (Single Nucleotide Polymorphisms) as listed in Table 5, (given according to their position(s) on the amino acid sequence, with the alternative amino acid(s) listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein HSAPHOL_P2 (SEQ ID NO: 37) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 5 Amino acid mutations SNP position(s) on amino acid sequence Alternative amino acid(s) Previously known SNP? 153 N -> S Yes 172 Q -> No 551 V -> A No 206 A -> No 272 R -> No 292 Y -> H Yes 342 V -> No 344 V -> No 354 K -> No 354 K -> Q No 380 E -> No

Variant protein HSAPHOL_P2 (SEQ ID NO: 37) is encoded by the following transcript(s): HSAPHOL_T4 (SEQ ID NO: 12), for which the sequence(s) is/are given at the end of the application. The coding portion of transcript HSAPHOL_T4 (SEQ ID NO: 12) is shown in bold; this coding portion starts at position 1 and ends at position 1659. The transcript also has the following SNPs as listed in Table 6 (given according to their position on the nucleotide sequence, with the alternative nucleic acid listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein HSAPHOL_P2 (SEQ ID NO: 37) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 6 Nucleic acid SNPs SNP position on nucleotide sequence Alternative nucleic acid Previously known SNP? 417 C -> T Yes 458 A -> G Yes 1140 G -> No 1509 C -> T Yes 1629 G -> T Yes 1652 T -> C No 1727 C -> T Yes 1788 G -> A Yes 1895 A -> C Yes 2050 C -> T Yes 2095 A -> G Yes 2240 G -> No 516 G -> No 2347 -> A No 2364 T -> G No 617 C -> No 815 G -> No 874 T -> C Yes 1026 G -> No 1032 G -> No 1060 A -> No 1060 A -> C No

Variant protein HSAPHOL_P3 (SEQ ID NO: 38) according to the present invention has an amino acid sequence as given at the end of the application; it is encoded by transcript(s) HSAPHOL_T5 (SEQ ID NO: 13). An alignment is given to the known protein (Alkaline phosphatase, tissue-nonspecific isozyme precursor) at the end of the application. One or more alignments to one or more previously published protein sequences are given at the end of the application. A brief description of the relationship of the variant protein according to the present invention to each such aligned protein is as follows:

Comparison report between HSAPHOL_P3 (SEQ ID NO: 38) and AAH21289:

1. An isolated chimeric polypeptide encoding for HSAPHOL_P3 (SEQ ID NO: 38), comprising a first amino acid sequence being at least 90% homologous to MISPFLVLAIGTCLTNSLVP corresponding to amino acids 63-82 of AAH21289, which also corresponds to amino acids 1-20 of HSAPHOL_P3 (SEQ ID NO: 38), and a second amino acid sequence being at least 90% homologous to GMGVSTVTAARILKGQLHHNPGEETRLEMDKFPFVALSKTYNTNAQVPDSAGTATAYL CGVKANEGTVGVSAATERSRCNTTQGNEVTSILRWAKDAGKSVGIVTTTRVNHATPSA AYAHSADRDWYSDNEMPPEALSQGCKDIAYQLMHNIRDIDVIMGGGRKYMYPKNKTD VEYESDEKARGTRLDGLDLVDTWKSFKPRYKHSHFIWNRTELLTLDPHNVDYLLGLFE PGDMQYELNRNNVTDPSLSEMVVVAIQILRKNPKGFFLLVEGGRIDHGHHEGKAKQAL HEAVEMDRAIGQAGSLTSSEDTLTVVTADHSHVFTFGGYTPRGNSIFGLAPMLSDTDKK PFTAILYGNGPGYKVVGGERENVSMVDYAHNNYQAQSAVPLRHETHGGEDVAVFSKG PMAHLLHGVHEQNYVPHVMAYAACIGANLGHCAPASSAGSLAAGPLLLALALYPLSV LF corresponding to amino acids 123-586 of AAH21289, which also corresponds to amino acids 21-484 of HSAPHOL_P3 (SEQ ID NO: 38), wherein said first and second amino acid sequences are contiguous and in a sequential order.

2. An isolated chimeric polypeptide encoding for an edge portion of HSAPHOL_P3 (SEQ ID NO: 38), comprising a polypeptide having a length “n”, wherein n is at least about 10 amino acids in length, optionally at least about 20 amino acids in length, preferably at least about 30 amino acids in length, more preferably at least about 40 amino acids in length and most preferably at least about 50 amino acids in length, wherein at least two amino acids comprise PG, having a structure as follows: a sequence starting from any of amino acid numbers 20-x to 20; and ending at any of amino acid numbers 21+((n−2)−x), in which x varies from 0 to n−2.

Comparison report between HSAPHOL_P3 (SEQ ID NO: 38) and PPBT_HUMAN:

1. An isolated chimeric polypeptide encoding for HSAPHOL_P3 (SEQ ID NO: 38), comprising a first amino acid sequence being at least 90% homologous to MISPFLVLAIGTCLTNSLVP corresponding to amino acids 1-20 of PPBT_HUMAN, which also corresponds to amino acids 1-20 of HSAPHOL_P3 (SEQ ID NO: 38), and a second amino acid sequence being at least 90% homologous to GMGVSTVTAARILKGQLHHNPGEETRLEMDKFPFVALSKTYNTNAQVPDSAGTATAYL CGVKANEGTVGVSAATERSRCNTTQGNEVTSILRWAKDAGKSVGIVTTTRVNHATPSA AYAHSADRDWYSDNEMPPEALSQGCKDIAYQLMHNIRDIDVIMGGGRKYMYPKNKTD VEYESDEKARGTRLDGLDLVDTWKSFKPRYKHSHFIWNRTELLTLDPHNVDYLLGLFE PGDMQYELNRNNVTDPSLSEMVVVAIQILRKNPKGFFLLVEGGRIDHGHHEGKAKQAL HEAVEMDRAIGQAGSLTSSEDTLTVVTADHSHVFTFGGYTPRGNSIFGLAPMLSDTDKK PFTAILYGNGPGYKVVGGERENVSMVDYAHNNYQAQSAVPLRHETHGGEDVAVFSKG PMAHLLHGVHEQNYVPHVMAYAACIGANLGHCAPASSAGSLAAGPLLLALALYPLSV LF corresponding to amino acids 61-524 of PPBT_HUMAN, which also corresponds to amino acids 21-484 of HSAPHOL_P3 (SEQ ID NO: 38), wherein said first and second amino acid sequences are contiguous and in a sequential order.

2. An isolated chimeric polypeptide encoding for an edge portion of HSAPHOL_P3 (SEQ ID NO: 38), comprising a polypeptide having a length “n”, wherein n is at least about 10 amino acids in length, optionally at least about 20 amino acids in length, preferably at least about 30 amino acids in length, more preferably at least about 40 amino acids in length and most preferably at least about 50 amino acids in length, wherein at least two amino acids comprise PG, having a structure as follows: a sequence starting from any of amino acid numbers 20-x to 20; and ending at any of amino acid numbers 21+((n−2)−x), in which x varies from 0 to n−2.

The location of the variant protein was determined according to results from a number of different software programs and analyses, including analyses from SignalP and other specialized programs. The variant protein is believed to be located as follows with regard to the cell: membrane. The protein localization is believed to be membrane because of manual inspection of known protein localization and/or gene structure, and/or similarity to known proteins.

Variant protein HSAPHOL_P3 (SEQ ID NO: 38) also has the following non-silent SNPs (Single Nucleotide Polymorphisms) as listed in Table 7, (given according to their position(s) on the amino acid sequence, with the alternative amino acid(s) listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein HSAPHOL_P3 (SEQ ID NO: 38) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 7 Amino acid mutations SNP position(s) on amino acid sequence Alternative amino acid(s) Previously known SNP? 103 Q -> No 137 A -> No 84 N -> S Yes 10 I -> No 203 R -> No 223 Y -> H Yes 273 V -> No 275 V -> No 285 K -> No 285 K -> Q No 311 E -> No 482 V -> A No

Variant protein HSAPHOL_P3 (SEQ ID NO: 38) is encoded by the following transcript(s): HSAPHOL_T5 (SEQ ID NO: 13), for which the sequence(s) is/are given at the end of the application. The coding portion of transcript HSAPHOL_T5 (SEQ ID NO: 13) is shown in bold; this coding portion starts at position 253 and ends at position 1704. The transcript also has the following SNPs as listed in Table 8 (given according to their position on the nucleotide sequence, with the alternative nucleic acid listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein HSAPHOL_P3 (SEQ ID NO: 38) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 8 Nucleic acid SNPs SNP position on nucleotide sequence Alternative nucleic acid Previously known SNP? 179 G -> C No 231 A -> No 1071 G -> No 1077 G -> No 1105 A -> No 1105 A -> C No 1185 G -> No 1554 C -> T Yes 1674 G -> T Yes 1697 T -> C No 1772 C -> T Yes 1833 G -> A Yes 232 A -> T No 1940 A -> C Yes 2095 C -> T Yes 2140 A -> G Yes 2285 G -> No 2392 -> A No 2409 T -> G No 281 T -> No 462 C -> T Yes 503 A -> G Yes 561 G -> No 662 C -> No 860 G -> No 919 T -> C Yes

Variant protein HSAPHOL_P4 (SEQ ID NO: 39) according to the present invention has an amino acid sequence as given at the end of the application; it is encoded by transcript(s) HSAPHOL_T6 (SEQ ID NO: 14). An alignment is given to the known protein (Alkaline phosphatase, tissue-nonspecific isozyme precursor) at the end of the application. One or more alignments to one or more previously published protein sequences are given at the end of the application. A brief description of the relationship of the variant protein according to the present invention to each such aligned protein is as follows:

Comparison report between HSAPHOL_P4 (SEQ ID NO: 39) and AAH21289:

1. An isolated chimeric polypeptide encoding for HSAPHOL_P4 (SEQ ID NO: 39), comprising a first amino acid sequence being at least 90% homologous to MGVSTVTAARILKGQLHHNPGEETRLEMDKFPFVALSKTYNTNAQVPDSAGTATAYLC GVKANEGTVGVSAATERSRCNTTQGNEVTSILRWAKDAGKSVGIVTTTRVNHATPSAA YAHSADRDWYSDNEMPPEALSQGCKDIAYQLMHNIRDIDVIMGGGRKYMYPKNKTDV EYESDEKARGTRLDGLDLVDTWKSFKPRYKHSHFIWNRTELLTLDPHNVDYLLGLFEP GDMQYELNRNNVTDPSLSEMVVVAIQILRKNPKGFFLLVEGGRIDHGHHEGKAKQALH EAVEMDRAIGQAGSLTSSEDTLTVVTADHSHVFTFGGYTPRGNSIFGLAPMLSDTDKKP FTAILYGNGPGYKVVGGERENVSMVDYAHNNYQAQSAVPLRHETHGGEDVAVFSKGP MAHLLHGVHEQNYVPHVMAYAACIGANLGHCAPASSAGSLAAGPLLLALALYPLSVL F corresponding to amino acids 124-586 of AAH21289, which also corresponds to amino acids 1-463 of HSAPHOL_P4 (SEQ ID NO: 39).

Comparison report between HSAPHOL_P4 (SEQ ID NO: 39) and PPBT_HUMAN:

1. An isolated chimeric polypeptide encoding for HSAPHOL_P4 (SEQ ID NO: 39), comprising a first amino acid sequence being at least 90% homologous to MGVSTVTAARILKGQLHHNPGEETRLEMDKFPFVALSKTYNTNAQVPDSAGTATAYLC GVKANEGTVGVSAATERSRCNTTQGNEVTSILRWAKDAGKSVGIVTTTRVNHATPSAA YAHSADRDWYSDNEMPPEALSQGCKDIAYQLMHNIRDIDVIMGGGRKYMYPKNKTDV EYESDEKARGTRLDGLDLVDTWKSFKPRYKHSHFIWNRTELLTLDPHNVDYLLGLFEP GDMQYELNRNNVTDPSLSEMVVVAIQILRKNPKGFFLLVEGGRIDHGHHEGKAKQALH EAVEMDRAIGQAGSLTSSEDTLTVVTADHSHVFTFGGYTPRGNSIFGLAPMLSDTDKKP FTAILYGNGPGYKVVGGERENVSMVDYAHNNYQAQSAVPLRHETHGGEDVAVFSKGP MAHLLHGVHEQNYVPHVMAYAACIGANLGHCAPASSAGSLAAGPLLLALALYPLSVL F corresponding to amino acids 62-524 of PPBT_HUMAN, which also corresponds to amino acids 1-463 of HSAPHOL_P4 (SEQ ID NO: 39).

The location of the variant protein was determined according to results from a number of different software programs and analyses, including analyses from SignalP and other specialized programs. The variant protein is believed to be located as follows with regard to the cell: membrane. The protein localization is believed to be membrane because only one of the two trans-membrane region prediction programs (Tmpred: 1, Tmhmm: 0) has predicted that this protein has a trans-membrane region, but similarity to known proteins suggests a GPI anchor. In addition both signal-peptide prediction programs predict that this protein is a non-secreted protein.

Variant protein HSAPHOL_P4 (SEQ ID NO: 39) also has the following non-silent SNPs (Single Nucleotide Polymorphisms) as listed in Table 9, (given according to their position(s) on the amino acid sequence, with the alternative amino acid(s) listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein HSAPHOL_P4 (SEQ ID NO: 39) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 9 Amino acid mutations SNP position(s) on amino acid sequence Alternative amino acid(s) Previously known SNP? 116 A -> No 182 R -> No 82 Q -> No 202 Y -> H Yes 252 V -> No 254 V -> No 264 K -> No 264 K -> Q No 290 E -> No 461 V -> A No 63 N -> S Yes

Variant protein HSAPHOL_P4 (SEQ ID NO: 39) is encoded by the following transcript(s): HSAPHOL_T6 (SEQ ID NO: 14), for which the sequence(s) is/are given at the end of the application. The coding portion of transcript HSAPHOL_T6 (SEQ ID NO: 14) is shown in bold; this coding portion starts at position 215 and ends at position 1603. The transcript also has the following SNPs as listed in Table 10 (given according to their position on the nucleotide sequence, with the alternative nucleic acid listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein HSAPHOL_P4 (SEQ ID NO: 39) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 10 Nucleic acid SNPs SNP position on nucleotide sequence Alternative nucleic acid Previously known SNP? 361 C -> T Yes 402 A -> G Yes 1084 G -> No 1453 C -> T Yes 1573 G -> T Yes 1596 T -> C No 1671 C -> T Yes 1732 G -> A Yes 1839 A -> C Yes 1994 C -> T Yes 2039 A -> G Yes 2184 G -> No 460 G -> No 2291 -> A No 2308 T -> G No 561 C -> No 759 G -> No 818 T -> C Yes 970 G -> No 976 G -> No 1004 A -> No 1004 A -> C No

Variant protein HSAPHOL_P5 (SEQ ID NO: 40) according to the present invention has an amino acid sequence as given at the end of the application; it is encoded by transcript(s) HSAPHOL_T7 (SEQ ID NO: 15). An alignment is given to the known protein (Alkaline phosphatase, tissue-nonspecific isozyme precursor) at the end of the application. One or more alignments to one or more previously published protein sequences are given at the end of the application. A brief description of the relationship of the variant protein according to the present invention to each such aligned protein is as follows:

Comparison report between HSAPHOL_P5 (SEQ ID NO: 40) and AAH21289:

1. An isolated chimeric polypeptide encoding for HSAPHOL_P5 (SEQ ID NO: 40), comprising a first amino acid sequence being at least 90% homologous to MISPFLVLAIGTCLTNSLVPEKEKDPKYWRDQAQETLKYALELQKLNTNVAKNVIMFL GDGMGVSTVTAARILKGQLHHNPGEETRLEMDKFPFVALSKTYNTNAQVPDSAGTAT AYLCGVKANEGTVGVSAATERSRCNTTQGNEVTSILRWAKDAGKSVGIVTTTRVNHA TPSAAYAHSADRDWYSDNEMPPEALSQGCKDIAYQLMHNIRDIDVIMGGGRKYMYPK NKTDVEYESDEKARGTRLDGLDLVDTWKSFKPRYKHSHFIWNRTELLTLDPHNVDYLL GLFEPGDMQYELNRNNVTDPSLSEMVVVAIQILRKNPKGFFLLVEGGRIDHGHHEGKA KQALHEAVEM corresponding to amino acids 63-417 of AAH21289, which also corresponds to amino acids 1-355 of HSAPHOL_P5 (SEQ ID NO: 40), and a second amino acid sequence being at least 90% homologous to DHSHVFTFGGYTPRGNSIFGLAPMLSDTDKKPFTAILYGNGPGYKVVGGERENVSMVD YAHNNYQAQSAVPLRHETHGGEDVAVFSKGPMAHLLHGVHEQNYVPHVMAYAACIG ANLGHCAPASSAGSLAAGPLLLALALYPLSVLF corresponding to amino acids 440-586 of AAH21289, which also corresponds to amino acids 356-502 of HSAPHOL_P5 (SEQ ID NO: 40), wherein said first and second amino acid sequences are contiguous and in a sequential order.

2. An isolated chimeric polypeptide encoding for an edge portion of HSAPHOL_P5 (SEQ ID NO: 40), comprising a polypeptide having a length “n”, wherein n is at least about 10 amino acids in length, optionally at least about 20 amino acids in length, preferably at least about 30 amino acids in length, more preferably at least about 40 amino acids in length and most preferably at least about 50 amino acids in length, wherein at least two amino acids comprise MD, having a structure as follows: a sequence starting from any of amino acid numbers 355-x to 355; and ending at any of amino acid numbers 356+((n−2)−x), in which x varies from 0 to n−2.

Comparison report between HSAPHOL_P5 (SEQ ID NO: 40) and PPBT_HUMAN:

1. An isolated chimeric polypeptide encoding for HSAPHOL_P5 (SEQ ID NO: 40), comprising a first amino acid sequence being at least 90% homologous to MISPFLVLAIGTCLTNSLVPEKEKDPKYWRDQAQETLKYALELQKLNTNVAKNVIMFL GDGMGVSTVTAARILKGQLHHNPGEETRLEMDKFPFVALSKTYNTNAQVPDSAGTAT AYLCGVKANEGTVGVSAATERSRCNTTQGNEVTSILRWAKDAGKSVGIVTTTRVNHA TPSAAYAHSADRDWYSDNEMPPEALSQGCKDIAYQLMHNIRDIDVIMGGGRKYMYPK NKTDVEYESDEKARGTRLDGLDLVDTWKSFKPRYKHSHFIWNRTELLTLDPHNVDYLL GLFEPGDMQYELNRNNVTDPSLSEMVVVAIQILRKNPKGFFLLVEGGRIDHGHHEGKA KQALHEAVEM corresponding to amino acids 1-355 of PPBT_HUMAN, which also corresponds to amino acids 1-355 of HSAPHOL_P5 (SEQ ID NO: 40), and a second amino acid sequence being at least 90% homologous to DHSHVFTFGGYTPRGNSIFGLAPMLSDTDKKPFTAILYGNGPGYKVVGGERENVSMVD YAHNNYQAQSAVPLRHETHGGEDVAVFSKGPMAHLLHGVHEQNYVPHVMAYAACIG ANLGHCAPASSAGSLAAGPLLLALALYPLSVLF corresponding to amino acids 377-524 of PPBT_HUMAN, which also corresponds to amino acids 356-502 of HSAPHOL_P5 (SEQ ID NO: 40), wherein said first and second amino acid sequences are contiguous and in a sequential order.

2. An isolated chimeric polypeptide encoding for an edge portion of HSAPHOL_P5 (SEQ ID NO: 40), comprising a polypeptide having a length “n”, wherein n is at least about 10 amino acids in length, optionally at least about 20 amino acids in length, preferably at least about 30 amino acids in length, more preferably at least about 40 amino acids in length and most preferably at least about 50 amino acids in length, wherein at least two amino acids comprise MD, having a structure as follows: a sequence starting from any of amino acid numbers 355-x to 355; and ending at any of amino acid numbers 356+((n−2)−x), in which x varies from 0 to n−2.

The location of the variant protein was determined according to results from a number of different software programs and analyses, including analyses from SignalP and other specialized programs. The variant protein is believed to be located as follows with regard to the cell: membrane. The protein localization is believed to be membrane because of manual inspection of known protein localization and/or gene structure and/or similarity to known protein.

Variant protein HSAPHOL_P5 (SEQ ID NO: 40) also has the following non-silent SNPs (Single Nucleotide Polymorphisms) as listed in Table 11, (given according to their position(s) on the amino acid sequence, with the alternative amino acid(s) listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein HSAPHOL_P5 (SEQ ID NO: 40) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 11 Amino acid mutations SNP position(s) on amino acid sequence Alternative amino acid(s) Previously known SNP? 124 N -> S Yes 143 Q -> No 500 V -> A No 10 I -> No 177 A -> No 243 R -> No 263 Y -> H Yes 313 V -> No 315 V -> No 325 K -> No 325 K -> Q No 351 E -> No

Variant protein HSAPHOL_P5 (SEQ ID NO: 40) is encoded by the following transcript(s): HSAPHOL_T7 (SEQ ID NO: 15), for which the sequence(s) is/are given at the end of the application. The coding portion of transcript HSAPHOL_T7 (SEQ ID NO: 15) is shown in bold; this coding portion starts at position 253 and ends at position 1758. The transcript also has the following SNPs as listed in Table 12 (given according to their position on the nucleotide sequence, with the alternative nucleic acid listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein HSAPHOL_P5 (SEQ ID NO: 40) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 12 Nucleic acid SNPs SNP position on nucleotide sequence Alternative nucleic acid Previously known SNP? 179 G -> C No 231 A -> No 1191 G -> No 1197 G -> No 1225 A -> No 1225 A -> C No 1305 G -> No 1608 C -> T Yes 1728 G -> T Yes 1751 T -> C No 1826 C -> T Yes 1887 G -> A Yes 232 A -> T No 1994 A -> C Yes 2149 C -> T Yes 2194 A -> G Yes 2339 G -> No 2446 -> A No 2463 T -> G No 281 T -> No 582 C -> T Yes 623 A -> G Yes 681 G -> No 782 C -> No 980 G -> No 1039 T -> C Yes

Variant protein HSAPHOL_P6 (SEQ ID NO: 41) according to the present invention has an amino acid sequence as given at the end of the application; it is encoded by transcript(s) HSAPHOL_T8 (SEQ ID NO: 16). An alignment is given to the known protein (Alkaline phosphatase, tissue-nonspecific isozyme precursor) at the end of the application. One or more alignments to one or more previously published protein sequences are given at the end of the application. A brief description of the relationship of the variant protein according to the present invention to each such aligned protein is as follows:

Comparison report between HSAPHOL_P6 (SEQ ID NO: 41) and AAH21289:

1. An isolated chimeric polypeptide encoding for HSAPHOL_P6 (SEQ ID NO: 41), comprising a first amino acid sequence being at least 90% homologous to MISPFLVLAIGTCLTNSLVPEKEKDPKYWRDQAQETLKYALELQKLNTNVAKNVIMFL GDGMGVSTVTAARILKGQLHHNPGEETRLEMDKFPFVALSKTYNTNAQVPDSAGTAT AYLCGVKANEGTVGVSAATERSRCNTTQGNEVTSILRWAKDAGKSVGIVTTTRVNHA TPSAAYAHSADRDWYSDNEMPPEALSQGCKDIAYQLMHNIRDIDVIMGGGRKYMYPK NKTDVEYESDEKARGTRLDGLDLVDTWKSFKPRYKHSHFIWNRTELLTLDPHNVDYLL corresponding to amino acids 63-349 of AAH21289, which also corresponds to amino acids 1-287 of HSAPHOL_P6 (SEQ ID NO: 41), and a second amino acid sequence being at least 90% homologous to GGRIDHGHHEGKAKQALHEAVEMDRAIGQAGSLTSSEDTLTVVTADHSHVFTFGGYTP RGNSIFGLAPMLSDTDKKPFTAILYGNGPGYKVVGGERENVSMVDYAHNNYQAQSAV PLRHETHGGEDVAVFSKGPMAHLLHGVHEQNYVPHVMAYAACIGANLGHCAPASSAG SLAAGPLLLALALYPLSVLF corresponding to amino acids 395-586 of AAH21289, which also corresponds to amino acids 288-479 of HSAPHOL_P6 (SEQ ID NO: 41), wherein said first and second amino acid sequences are contiguous and in a sequential order.

2. An isolated chimeric polypeptide encoding for an edge portion of HSAPHOL_P6 (SEQ ID NO: 41), comprising a polypeptide having a length “n”, wherein n is at least about 10 amino acids in length, optionally at least about 20 amino acids in length, preferably at least about 30 amino acids in length, more preferably at least about 40 amino acids in length and most preferably at least about 50 amino acids in length, wherein at least two amino acids comprise LG, having a structure as follows: a sequence starting from any of amino acid numbers 287-x to 287; and ending at any of amino acid numbers 288+((n−2)−x), in which x varies from 0 to n−2.

Comparison report between HSAPHOL_P6 (SEQ ID NO: 41) and PPBT_HUMAN:

1. An isolated chimeric polypeptide encoding for HSAPHOL_P6 (SEQ ID NO: 41), comprising a first amino acid sequence being at least 90% homologous to MISPFLVLAIGTCLTNSLVPEKEKDPKYWRDQAQETLKYALELQKLNTNVAKNVIMFL GDGMGVSTVTAARILKGQLHHNPGEETRLEMDKFPFVALSKTYNTNAQVPDSAGTAT AYLCGVKANEGTVGVSAATERSRCNTTQGNEVTSILRWAKDAGKSVGIVTTTRVNHA TPSAAYAHSADRDWYSDNEMPPEALSQGCKDIAYQLMHNIRDIDVIMGGGRKYMYPK NKTDVEYESDEKARGTRLDGLDLVDTWKSFKPRYKHSHFIWNRTELLTLDPHNVDYLL corresponding to amino acids 1-287 of PPBT_HUMAN, which also corresponds to amino acids 1-287 of HSAPHOL_P6 (SEQ ID NO: 41), and a second amino acid sequence being at least 90% homologous to GGRIDHGHHEGKAKQALHEAVEMDRAIGQAGSLTSSEDTLTVVTADHSHVFTFGGYTP RGNSIFGLAPMLSDTDKKPFTAILYGNGPGYKVVGGERENVSMVDYAHNNYQAQSAV PLRHETHGGEDVAVFSKGPMAHLLHGVHEQNYVPHVMAYAACIGANLGHCAPASSAG SLAAGPLLLALALYPLSVLF corresponding to amino acids 333-524 of PPBT_HUMAN, which also corresponds to amino acids 288-479 of HSAPHOL_P6 (SEQ ID NO: 41), wherein said first and second amino acid sequences are contiguous and in a sequential order.

2. An isolated chimeric polypeptide encoding for an edge portion of HSAPHOL_P6 (SEQ ID NO: 41), comprising a polypeptide having a length “n”, wherein n is at least about 10 amino acids in length, optionally at least about 20 amino acids in length, preferably at least about 30 amino acids in length, more preferably at least about 40 amino acids in length and most preferably at least about 50 amino acids in length, wherein at least two amino acids comprise LG, having a structure as follows: a sequence starting from any of amino acid numbers 287-x to 287; and ending at any of amino acid numbers 288+((n−2)−x), in which x varies from 0 to n−2.

The location of the variant protein was determined according to results from a number of different software programs and analyses, including analyses from SignalP and other specialized programs. The variant protein is believed to be located as follows with regard to the cell: membrane. The protein localization is believed to be membrane because both signal-peptide prediction programs predict that this protein has a signal peptide, and at least one of two trans-membrane region prediction programs predicts that this protein has a trans-membrane region, also similarity to known proteins suggests a GPI anchor.

Variant protein HSAPHOL_P6 (SEQ ID NO: 41) also has the following non-silent SNPs (Single Nucleotide Polymorphisms) as listed in Table 13, (given according to their position(s) on the amino acid sequence, with the alternative amino acid(s) listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein HSAPHOL_P6 (SEQ ID NO: 41) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 13 Amino acid mutations SNP position(s) on amino acid sequence Alternative amino acid(s) Previously known SNP? 124 N -> S Yes 143 Q -> No 177 A -> No 243 R -> No 263 Y -> H Yes 306 E -> No 477 V -> A No 10 I -> No

Variant protein HSAPHOL_P6 (SEQ ID NO: 41) is encoded by the following transcript(s): HSAPHOL_T8 (SEQ ID NO: 16), for which the sequence(s) is/are given at the end of the application. The coding portion of transcript HSAPHOL_T8 (SEQ ID NO: 16) is shown in bold; this coding portion starts at position 253 and ends at position 1689. The transcript also has the following SNPs as listed in Table 14 (given according to their position on the nucleotide sequence, with the alternative nucleic acid listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein HSAPHOL_P6 (SEQ ID NO: 41) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 14 Nucleic acid SNPs SNP position on nucleotide sequence Alternative nucleic acid Previously known SNP? 179 G -> C No 231 A -> No 1170 G -> No 1539 C -> T Yes 1659 G -> T Yes 1682 T -> C No 1757 C -> T Yes 1818 G -> A Yes 1925 A -> C Yes 2080 C -> T Yes 2125 A -> G Yes 2270 G -> No 232 A -> T No 2377 -> A No 2394 T -> G No 281 T -> No 582 C -> T Yes 623 A -> G Yes 681 G -> No 782 C -> No 980 G -> No 1039 T -> C Yes

Variant protein HSAPHOL_P7 (SEQ ID NO: 42) according to the present invention has an amino acid sequence as given at the end of the application; it is encoded by transcript(s) HSAPHOL_T9 (SEQ ID NO: 17). An alignment is given to the known protein (Alkaline phosphatase, tissue-nonspecific isozyme precursor) at the end of the application. One or more alignments to one or more previously published protein sequences are given at the end of the application. A brief description of the relationship of the variant protein according to the present invention to each such aligned protein is as follows:

Comparison report between HSAPHOL_P7 (SEQ ID NO: 42) and AAH21289:

1. An isolated chimeric polypeptide encoding for HSAPHOL_P7 (SEQ ID NO: 42), comprising a first amino acid sequence being at least 90% homologous to MISPFLVLAIGTCLTNSLVPEKEKDPKYWRDQAQETLKYALELQKLNTNVAKNVIMFL GDGMGVSTVTAARILKGQLHHNPGEETRLEMDKFPFVALSKTYNTNAQVPDSAGTAT AYLCGVKANEGTVGVSAATERSRCNTTQGNEVTSILRWAKDAGKSVGIVTTTRVNHA TPSAAYAHSADRDWYSDNEMPPEALSQGCKDIAYQLMHNIRDIDVIMGGGRKYMYPK NKTDVEYESDEKARGTRLDGLDLVDTWKSFKPRYK corresponding to amino acids 63-326 of AAH21289, which also corresponds to amino acids 1-264 of HSAPHOL_P7 (SEQ ID NO: 42), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence LPPRCPLANRVDFSWAGREYRLQTFSKPLIFLANVFLQTQRP (SEQ ID NO: 1112) corresponding to amino acids 265-306 of HSAPHOL_P7 (SEQ ID NO: 42), wherein said first and second amino acid sequences are contiguous and in a sequential order.

2. An isolated polypeptide encoding for a tail of HSAPHOL_P7 (SEQ ID NO: 42), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence LPPRCPLANRVDFSWAGREYRLQTFSKPLIFLANVFLQTQRP (SEQ ID NO: 1112) in HSAPHOL_P7 (SEQ ID NO: 42).

Comparison report between HSAPHOL_P7 (SEQ ID NO: 42) and PPBT_HUMAN:

1. An isolated chimeric polypeptide encoding for HSAPHOL_P7 (SEQ ID NO: 42), comprising a first amino acid sequence being at least 90% homologous to MISPFLVLAIGTCLTNSLVPEKEKDPKYWRDQAQETLKYALELQKLNTNVAKNVIMFL GDGMGVSTVTAARILKGQLHHNPGEETRLEMDKFPFVALSKTYNTNAQVPDSAGTAT AYLCGVKANEGTVGVSAATERSRCNTTQGNEVTSILRWAKDAGKSVGIVTTTRVNHA TPSAAYAHSADRDWYSDNEMPPEALSQGCKDIAYQLMHNIRDIDVIMGGGRKYMYPK NKTDVEYESDEKARGTRLDGLDLVDTWKSFKPR corresponding to amino acids 1-262 of PPBT_HUMAN, which also corresponds to amino acids 1-262 of HSAPHOL_P7 (SEQ ID NO: 42), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence YKLPPRCPLANRVDFSWAGREYRLQTFSKPLIFLANVFLQTQRP corresponding to amino acids 263-306 of HSAPHOL_P7 (SEQ ID NO: 42), wherein said first and second amino acid sequences are contiguous and in a sequential order.

2. An isolated polypeptide encoding for a tail of HSAPHOL_P7 (SEQ ID NO: 42), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence YKLPPRCPLANRVDFSWAGREYRLQTFSKPLIFLANVFLQTQRP in HSAPHOL_P7 (SEQ ID NO: 42).

Comparison report between HSAPHOL_P7 (SEQ ID NO: 42) and O75090 (SEQ ID NO: 958):

1. An isolated chimeric polypeptide encoding for HSAPHOL_P7 (SEQ ID NO: 42), comprising a first amino acid sequence being at least 90% homologous to MISPFLVLAIGTCLTNSLVPEKEKDPKYWRDQAQETLKYALELQKLNTNVAKNVIMFL GDGMGVSTVTAARILKGQLHHNPGEETRLEMDKFPFVALSKTYNTNAQVPDSAGTAT AYLCGVKANEGTVGVSAATERSRCNTTQGNEVTSILRWAKDAGKSVGIVTTTRVNHA TPSAAYAHSADRDWYSDNEMPPEALSQGCKDIAYQLMHNIRDIDVIMGGGRKYMYPK NKTDVEYESDEKARGTRLDGLDLVDTWKSFKPRYK corresponding to amino acids 1-264 of O75090 (SEQ ID NO: 958), which also corresponds to amino acids 1-264 of HSAPHOL_P7 (SEQ ID NO: 42), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence LPPRCPLANRVDFSWAGREYRLQTFSKPLIFLANVFLQTQRP corresponding to amino acids 265-306 of HSAPHOL_P7 (SEQ ID NO: 42), wherein said first and second amino acid sequences are contiguous and in a sequential order.

2. An isolated polypeptide encoding for a tail of HSAPHOL_P7 (SEQ ID NO: 42), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence LPPRCPLANRVDFSWAGREYRLQTFSKPLIFLANVFLQTQRP (SEQ ID NO: 1112) in HSAPHOL_P7 (SEQ ID NO: 42).

The location of the variant protein was determined according to results from a number of different software programs and analyses, including analyses from SignalP and other specialized programs. The variant protein is believed to be located as follows with regard to the cell: secreted. The protein localization is believed to be secreted because both signal-peptide prediction programs predict that this protein has a signal peptide, and neither trans-membrane region prediction program predicts that this protein has a trans-membrane region.

Variant protein HSAPHOL_P7 (SEQ ID NO: 42) also has the following non-silent SNPs (Single Nucleotide Polymorphisms) as listed in Table 15, (given according to their position(s) on the amino acid sequence, with the alternative amino acid(s) listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein HSAPHOL_P7 (SEQ ID NO: 42) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 15 Amino acid mutations SNP position(s) on amino acid sequence Alternative amino acid(s) Previously known SNP? 124 N -> S Yes 143 Q -> No 177 A -> No 243 R -> No 263 Y -> H Yes 273 N -> T Yes 10 I -> No

Variant protein HSAPHOL_P7 (SEQ ID NO: 42) is encoded by the following transcript(s): HSAPHOL_T9 (SEQ ID NO: 17), for which the sequence(s) is/are given at the end of the application. The coding portion of transcript HSAPHOL_T9 (SEQ ID NO: 17) is shown in bold; this coding portion starts at position 253 and ends at position 1170. The transcript also has the following SNPs as listed in Table 16 (given according to their position on the nucleotide sequence, with the alternative nucleic acid listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein HSAPHOL_P7 (SEQ ID NO: 42) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 16 Nucleic acid SNPs SNP position on nucleotide sequence Alternative nucleic acid Previously known SNP? 179 G -> C No 231 A -> No 1070 A -> C Yes 1225 C -> T Yes 1270 A -> G Yes 1415 G -> No 1522 -> A No 1539 T -> G No 232 A -> T No 281 T -> No 582 C -> T Yes 623 A -> G Yes 681 G -> No 782 C -> No 980 G -> No 1039 T -> C Yes

Variant protein HSAPHOL_P8 (SEQ ID NO: 43) according to the present invention has an amino acid sequence as given at the end of the application; it is encoded by transcript(s) HSAPHOL_T10 (SEQ ID NO: 11). An alignment is given to the known protein (Alkaline phosphatase, tissue-nonspecific isozyme precursor) at the end of the application. One or more alignments to one or more previously published protein sequences are given at the end of the application. A brief description of the relationship of the variant protein according to the present invention to each such aligned protein is as follows:

Comparison report between HSAPHOL_P8 (SEQ ID NO: 43) and AAH21289:

1. An isolated chimeric polypeptide encoding for HSAPHOL_P8 (SEQ ID NO: 43), comprising a first amino acid sequence being at least 90% homologous to MISPFLVLAIGTCLTNSLVPEKEKDPKYWRDQAQETLKYALELQKLNTNVAKNVIMFL GDGMGVSTVTAARILKGQLHHNPGEETRLEMDKFPFVALSKTYNTNAQVPDSAGTAT AYLCGVKANEGTVGVSAATERSRCNTTQGNEVTSILRWAKDAGKSVGIVTTTRVNHA TPSAAYAHSADRDWYSDNEMPPEALSQGCKDIAYQLMHNIRDIDVIMGGGRKYMYPK NKTDVEYESDEKARGTRLDGLDLVDTWKSFKPRYKHSHFIWNRTELLTLDPHNVDYLL G corresponding to amino acids 63-350 of AAH21289, which also corresponds to amino acids 1-288 of HSAPHOL_P8 (SEQ ID NO: 43), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence KWRGWRGGCMARSLVAGAACGQHLGTRP (SEQ ID NO: 1113) corresponding to amino acids 289-316 of HSAPHOL_P8 (SEQ ID NO: 43), wherein said first and second amino acid sequences are contiguous and in a sequential order.

2. An isolated polypeptide encoding for a tail of HSAPHOL_P8 (SEQ ID NO: 43), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence KWRGWRGGCMARSLVAGAACGQHLGTRP (SEQ ID NO: 1113) in HSAPHOL_P8 (SEQ ID NO: 43).

Comparison report between HSAPHOL_P8 (SEQ ID NO: 43) and PPBT_HUMAN:

1. An isolated chimeric polypeptide encoding for HSAPHOL_P8 (SEQ ID NO: 43), comprising a first amino acid sequence being at least 90% homologous to MISPFLVLAIGTCLTNSLVPEKEKDPKYWRDQAQETLKYALELQKLNTNVAKNVIMFL GDGMGVSTVTAARILKGQLHHNPGEETRLEMDKFPFVALSKTYNTNAQVPDSAGTAT AYLCGVKANEGTVGVSAATERSRCNTTQGNEVTSILRWAKDAGKSVGIVTTTRVNHA TPSAAYAHSADRDWYSDNEMPPEALSQGCKDIAYQLMHNIRDIDVIMGGGRKYMYPK NKTDVEYESDEKARGTRLDGLDLVDTWKSFKPRYKHSHFIWNRTELLTLDPHNVDYLL G corresponding to amino acids 1-288 of PPBT_HUMAN, which also corresponds to amino acids 1-288 of HSAPHOL_P8 (SEQ ID NO: 43), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence KWRGWRGGCMARSLVAGAACGQHLGTRP (SEQ ID NO: 1113) corresponding to amino acids 289-316 of HSAPHOL_P8 (SEQ ID NO: 43), wherein said first and second amino acid sequences are contiguous and in a sequential order.

2. An isolated polypeptide encoding for a tail of HSAPHOL_P8 (SEQ ID NO: 43), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence KWRGWRGGCMARSLVAGAACGQHLGTRP (SEQ ID NO: 1113) in HSAPHOL_P8 (SEQ ID NO: 43).

Comparison report between HSAPHOL_P8 (SEQ ID NO: 43) and O75090 (SEQ ID NO: 958) (SEQ ID NO:958):

1. An isolated chimeric polypeptide encoding for HSAPHOL_P8 (SEQ ID NO: 43), comprising a first amino acid sequence being at least 90% homologous to MISPFLVLAIGTCLTNSLVPEKEKDPKYWRDQAQETLKYALELQKLNTNVAKNVIMFL GDGMGVSTVTAARILKGQLHHNPGEETRLEMDKFPFVALSKTYNTNAQVPDSAGTAT AYLCGVKANEGTVGVSAATERSRCNTTQGNEVTSILRWAKDAGKSVGIVTTTRVNHA TPSAAYAHSADRDWYSDNEMPPEALSQGCKDIAYQLMHNIRDIDVIMGGGRKYMYPK NKTDVEYESDEKARGTRLDGLDLVDTWKSFKPRYKHSHFIWNRTELLTLDPHNVDYLL G corresponding to amino acids 1-288 of O75090 (SEQ ID NO: 958), which also corresponds to amino acids 1-288 of HSAPHOL_P8 (SEQ ID NO: 43), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence KWRGWRGGCMARSLVAGAACGQHLGTRP (SEQ ID NO: 1113) corresponding to amino acids 289-316 of HSAPHOL_P8 (SEQ ID NO: 43), wherein said first and second amino acid sequences are contiguous and in a sequential order.

2. An isolated polypeptide encoding for a tail of HSAPHOL_P8 (SEQ ID NO: 43), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence KWRGWRGGCMARSLVAGAACGQHLGTRP (SEQ ID NO: 1113) in HSAPHOL_P8 (SEQ ID NO: 43).

The location of the variant protein was determined according to results from a number of different software programs and analyses, including analyses from SignalP and other specialized programs. The variant protein is believed to be located as follows with regard to the cell: secreted. The protein localization is believed to be secreted because both signal-peptide prediction programs predict that this protein has a signal peptide, and neither trans-membrane region prediction program predicts that this protein has a trans-membrane region.

Variant protein HSAPHOL_P8 (SEQ ID NO: 43) also has the following non-silent SNPs (Single Nucleotide Polymorphisms) as listed in Table 17, (given according to their position(s) on the amino acid sequence, with the alternative amino acid(s) listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein HSAPHOL_P8 (SEQ ID NO: 43) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 17 Amino acid mutations SNP position(s) on amino acid sequence Alternative amino acid(s) Previously known SNP? 124 N -> S Yes 143 Q -> No 177 A -> No 243 R -> No 263 Y -> H Yes 294 R -> S Yes 305 G -> R Yes 307 A -> V Yes 10 I -> No

Variant protein HSAPHOL_P8 (SEQ ID NO: 43) is encoded by the following transcript(s): HSAPHOL_T10 (SEQ ID NO: 11), for which the sequence(s) is/are given at the end of the application. The coding portion of transcript HSAPHOL_T10 (SEQ ID NO: 11) is shown in bold; this coding portion starts at position 253 and ends at position 1200. The transcript also has the following SNPs as listed in Table 18 (given according to their position on the nucleotide sequence, with the alternative nucleic acid listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein HSAPHOL_P8 (SEQ ID NO: 43) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 18 Nucleic acid SNPs SNP position on nucleotide sequence Alternative nucleic acid Previously known SNP? 179 G -> C No 231 A -> No 1134 G -> T Yes 1165 G -> A Yes 1172 C -> T Yes 1376 T -> C Yes 1384 G -> C Yes 1565 T -> G Yes 232 A -> T No 281 T -> No 582 C -> T Yes 623 A -> G Yes 681 G -> No 782 C -> No 980 G -> No 1039 T -> C Yes

As noted above, cluster HSAPHOL features 18 segment(s), which were listed in Table 2 above and for which the sequence(s) are given at the end of the application. These segment(s) are portions of nucleic acid sequence(s) which are described herein separately because they are of particular interest. A description of each segment according to the present invention is now provided.

Segment cluster HSAPHOL_node_(—)11 (SEQ ID NO: 18) according to the present invention is supported by 48 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HSAPHOL_T10 (SEQ ID NO: 11), HSAPHOL_T5 (SEQ ID NO: 13), HSAPHOL_T7 (SEQ ID NO: 15), HSAPHOL_T8 (SEQ ID NO: 16) and HSAPHOL_T9 (SEQ ID NO: 17). Table 19 below describes the starting and ending position of this segment on each transcript.

TABLE 19 Segment location on transcripts Segment Segment Transcript starting ending name position position HSAPHOL_T10 149 313 (SEQ ID NO:11) HSAPHOL_T5 149 313 (SEQ ID NO:13) HSAPHOL_T7 149 313 (SEQ ID NO:15) HSAPHOL_T8 149 313 (SEQ ID NO:16) HSAPHOL_T9 149 313 (SEQ ID NO:17)

Segment cluster HSAPHOL_node_(—)13 (SEQ ID NO: 19) according to the present invention is supported by 50 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HSAPHOL_T10 (SEQ ID NO: 11), HSAPHOL_T4 (SEQ ID NO: 12), HSAPHOL_T7 (SEQ ID NO: 15), HSAPHOL_T8 (SEQ ID NO: 16) and HSAPHOL_T9 (SEQ ID NO: 17). Table 20 below describes the starting and ending position of this segment on each transcript.

TABLE 20 Segment location on transcripts Segment Segment Transcript starting ending name position position HSAPHOL_T10 314 433 (SEQ ID NO:11) HSAPHOL_T4 149 268 (SEQ ID NO:12) HSAPHOL_T7 314 433 (SEQ ID NO:15) HSAPHOL_T8 314 433 (SEQ ID NO:16) HSAPHOL_T9 314 433 (SEQ ID NO:17)

Segment cluster HSAPHOL_node_(—)15 (SEQ ID NO: 20) according to the present invention is supported by 1 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HSAPHOL_T6 (SEQ ID NO: 14). Table 21 below describes the starting and ending position of this segment on each transcript.

TABLE 21 Segment location on transcripts Segment Segment Transcript starting ending name position position HSAPHOL_T6 1 212 (SEQ ID NO:14)

Segment cluster HSAPHOL_node_(—)19 (SEQ ID NO: 21) according to the present invention is supported by 46 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HSAPHOL_T10 (SEQ ID NO: 11), HSAPHOL_T4 (SEQ ID NO: 12), HSAPHOL_T5 (SEQ ID NO: 13), HSAPHOL_T6 (SEQ ID NO: 14), HSAPHOL_T7 (SEQ ID NO: 15), HSAPHOL_T8 (SEQ ID NO: 16) and HSAPHOL_T9 (SEQ ID NO: 17). Table 22 below describes the starting and ending position of this segment on each transcript.

TABLE 22 Segment location on transcripts Segment Segment Transcript starting ending name position position HSAPHOL_T10 550 724 (SEQ ID NO:11) HSAPHOL_T4 385 559 (SEQ ID NO:12) HSAPHOL_T5 430 604 (SEQ ID NO:13) HSAPHOL_T6 329 503 (SEQ ID NO:14) HSAPHOL_T7 550 724 (SEQ ID NO:15) HSAPHOL_T8 550 724 (SEQ ID NO:16) HSAPHOL_T9 550 724 (SEQ ID NO:17)

Segment cluster HSAPHOL_node_(—)2 (SEQ ID NO: 22) according to the present invention is supported by 33 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HSAPHOL_T10 (SEQ ID NO: 11), HSAPHOL_T4 (SEQ ID NO: 12), HSAPHOL_T5 (SEQ ID NO: 13), HSAPHOL_T7 (SEQ ID NO: 15), HSAPHOL_T8 (SEQ ID NO: 16) and HSAPHOL_T9 (SEQ ID NO: 17). Table 23 below describes the starting and ending position of this segment on each transcript.

TABLE 23 Segment location on transcripts Segment Segment Transcript starting ending name position position HSAPHOL_T10 1 148 (SEQ ID NO:11) HSAPHOL_T4 1 148 (SEQ ID NO:12) HSAPHOL_T5 1 148 (SEQ ID NO:13) HSAPHOL_T7 1 148 (SEQ ID NO:15) HSAPHOL_T8 1 148 (SEQ ID NO:16) HSAPHOL_T9 1 148 (SEQ ID NO:17)

Segment cluster HSAPHOL_node_(—)2 (SEQ ID NO: 22)1 according to the present invention is supported by 45 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HSAPHOL_T10 (SEQ ID NO: 11), HSAPHOL_T4 (SEQ ID NO: 12), HSAPHOL_T5 (SEQ ID NO: 13), HSAPHOL_T6 (SEQ ID NO: 14), HSAPHOL_T7 (SEQ ID NO: 15), HSAPHOL_T8 (SEQ ID NO: 16) and HSAPHOL_T9 (SEQ ID NO: 17). Table 24 below describes the starting and ending position of this segment on each transcript.

TABLE 24 Segment location on transcripts Segment Segment Transcript starting ending name position position HSAPHOL_T10 725 900 (SEQ ID NO:11) HSAPHOL_T4 560 735 (SEQ ID NO:12) HSAPHOL_T5 605 780 (SEQ ID NO:13) HSAPHOL_T6 504 679 (SEQ ID NO:14) HSAPHOL_T7 725 900 (SEQ ID NO:15) HSAPHOL_T8 725 900 (SEQ ID NO:16) HSAPHOL_T9 725 900 (SEQ ID NO:17)

Segment cluster HSAPHOL_node_(—)2 (SEQ ID NO: 22)3 according to the present invention is supported by 45 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HSAPHOL_T10 (SEQ ID NO: 11), HSAPHOL_T4 (SEQ ID NO: 12), HSAPHOL_T5 (SEQ ID NO: 13), HSAPHOL_T6 (SEQ ID NO: 14), HSAPHOL_T7 (SEQ ID NO: 15), HSAPHOL_T8 (SEQ ID NO: 16) and HSAPHOL_T9 (SEQ ID NO: 17). Table 25 below describes the starting and ending position of this segment on each transcript.

TABLE 25 Segment location on transcripts Segment Segment Transcript starting ending name position position HSAPHOL_T10 901 1044 (SEQ ID NO:11) HSAPHOL_T4 736 879 (SEQ ID NO:12) HSAPHOL_T5 781 924 (SEQ ID NO:13) HSAPHOL_T6 680 823 (SEQ ID NO:14) HSAPHOL_T7 901 1044 (SEQ ID NO:15) HSAPHOL_T8 901 1044 (SEQ ID NO:16) HSAPHOL_T9 901 1044 (SEQ ID NO:17)

Segment cluster HSAPHOL_node_(—)2 (SEQ ID NO: 22)6 according to the present invention is supported by 2 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HSAPHOL_T10 (SEQ ID NO: 11). Table 26 below describes the starting and ending position of this segment on each transcript.

TABLE 26 Segment location on transcripts Segment Segment Transcript starting ending name position position HSAPHOL_T10 1115 1572 (SEQ ID NO:11)

Segment cluster HSAPHOL_node_(—)2 (SEQ ID NO: 22)8 according to the present invention is supported by 44 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HSAPHOL_T4 (SEQ ID NO: 12), HSAPHOL_T5 (SEQ ID NO: 13), HSAPHOL_T6 (SEQ ID NO: 14) and HSAPHOL_T7 (SEQ ID NO: 15). Table 27 below describes the starting and ending position of this segment on each transcript.

TABLE 27 Segment location on transcripts Segment Segment Transcript starting ending name position position HSAPHOL_T4 950 1084 (SEQ ID NO:12) HSAPHOL_T5 995 1129 (SEQ ID NO:13) HSAPHOL_T6 894 1028 (SEQ ID NO:14) HSAPHOL_T7 1115 1249 (SEQ ID NO:15)

Segment cluster HSAPHOL_node_(—)38 (SEQ ID NO: 27) according to the present invention is supported by 45 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HSAPHOL_T4 (SEQ ID NO: 12), HSAPHOL_T5 (SEQ ID NO: 13), HSAPHOL_T6 (SEQ ID NO: 14), HSAPHOL_T7 (SEQ ID NO: 15) and HSAPHOL_T8 (SEQ ID NO: 16). Table 28 below describes the starting and ending position of this segment on each transcript.

TABLE 28 Segment location on transcripts Segment Segment Transcript starting ending name position position HSAPHOL_T4 1277 1396 (SEQ ID NO:12) HSAPHOL_T5 1322 1441 (SEQ ID NO:13) HSAPHOL_T6 1221 1340 (SEQ ID NO:14) HSAPHOL_T7 1376 1495 (SEQ ID NO:15) HSAPHOL_T8 1307 1426 (SEQ ID NO:16)

Segment cluster HSAPHOL_node_(—)40 (SEQ ID NO: 28) according to the present invention is supported by 69 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HSAPHOL_T4 (SEQ ID NO: 12), HSAPHOL_T5 (SEQ ID NO: 13), HSAPHOL_T6 (SEQ ID NO: 14), HSAPHOL_T7 (SEQ ID NO: 15) and HSAPHOL_T8 (SEQ ID NO: 16). Table 29 below describes the starting and ending position of this segment on each transcript.

TABLE 29 Segment location on transcripts Segment Segment Transcript starting ending name position position HSAPHOL_T4 1397 1759 (SEQ ID NO:12) HSAPHOL_T5 1442 1804 (SEQ ID NO:13) HSAPHOL_T6 1341 1703 (SEQ ID NO:14) HSAPHOL_T7 1496 1858 (SEQ ID NO:15) HSAPHOL_T8 1427 1789 (SEQ ID NO:16)

Segment cluster HSAPHOL_node_(—)42 (SEQ ID NO: 29) according to the present invention is supported by 99 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HSAPHOL_T4 (SEQ ID NO: 12), HSAPHOL_T5 (SEQ ID NO: 13), HSAPHOL_T6 (SEQ ID NO: 14), HSAPHOL_T7 (SEQ ID NO: 15), HSAPHOL_T8 (SEQ ID NO: 16) and HSAPHOL_T9 (SEQ ID NO: 17). Table 30 below describes the starting and ending position of this segment on each transcript.

TABLE 30 Segment location on transcripts Segment Segment Transcript starting ending name position position HSAPHOL_T4 1870 2426 (SEQ ID NO:12) HSAPHOL_T5 1915 2471 (SEQ ID NO:13) HSAPHOL_T6 1814 2370 (SEQ ID NO:14) HSAPHOL_T7 1969 2525 (SEQ ID NO:15) HSAPHOL_T8 1900 2456 (SEQ ID NO:16) HSAPHOL_T9 1045 1601 (SEQ ID NO:17)

According to an optional embodiment of the present invention, short segments related to the above cluster are also provided. These segments are up to about 120 bp in length, and so are included in a separate description.

Segment cluster HSAPHOL_node_(—)16 (SEQ ID NO: 30) according to the present invention is supported by 46 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HSAPHOL_T10 (SEQ ID NO: 11), HSAPHOL_T4 (SEQ ID NO: 12), HSAPHOL_T5 (SEQ ID NO: 13), HSAPHOL_T6 (SEQ ID NO: 14), HSAPHOL_T7 (SEQ ID NO: 15), HSAPHOL_T8 (SEQ ID NO: 16) and HSAPHOL_T9 (SEQ ID NO: 17). Table 31 below describes the starting and ending position of this segment on each transcript.

TABLE 31 Segment location on transcripts Segment Segment Transcript starting ending name position position HSAPHOL_T10 434 549 (SEQ ID NO:11) HSAPHOL_T4 269 384 (SEQ ID NO:12) HSAPHOL_T5 314 429 (SEQ ID NO:13) HSAPHOL_T6 213 328 (SEQ ID NO:14) HSAPHOL_T7 434 549 (SEQ ID NO:15) HSAPHOL_T8 434 549 (SEQ ID NO:16) HSAPHOL_T9 434 549 (SEQ ID NO:17)

Segment cluster HSAPHOL_node_(—)2 (SEQ ID NO: 22)5 according to the present invention is supported by 39 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HSAPHOL_T10 (SEQ ID NO: 11), HSAPHOL_T4 (SEQ ID NO: 12), HSAPHOL_T5 (SEQ ID NO: 13), HSAPHOL_T6 (SEQ ID NO: 14), HSAPHOL_T7 (SEQ ID NO: 15) and HSAPHOL_T8 (SEQ ID NO: 16). Table 32 below describes the starting and ending position of this segment on each transcript.

TABLE 32 Segment location on transcripts Segment Segment Transcript starting ending name position position HSAPHOL_T10 1045 1114 (SEQ ID NO:11) HSAPHOL_T4 880 949 (SEQ ID NO:12) HSAPHOL_T5 925 994 (SEQ ID NO:13) HSAPHOL_T6 824 893 (SEQ ID NO:14) HSAPHOL_T7 1045 1114 (SEQ ID NO:15) HSAPHOL_T8 1045 1114 (SEQ ID NO:16)

Segment cluster HSAPHOL_node_(—)34 (SEQ ID NO: 32) according to the present invention is supported by 48 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HSAPHOL_T4 (SEQ ID NO: 12), HSAPHOL_T5 (SEQ ID NO: 13), HSAPHOL_T6 (SEQ ID NO: 14), HSAPHOL_T7 (SEQ ID NO: 15) and HSAPHOL_T8 (SEQ ID NO: 16). Table 33 below describes the starting and ending position of this segment on each transcript.

TABLE 33 Segment location on transcripts Segment Segment Transcript starting ending name position position HSAPHOL_T4 1085 1155 (SEQ ID NO:12) HSAPHOL_T5 1130 1200 (SEQ ID NO:13) HSAPHOL_T6 1029 1099 (SEQ ID NO:14) HSAPHOL_T7 1250 1320 (SEQ ID NO:15) HSAPHOL_T8 1115 1185 (SEQ ID NO:16)

Segment cluster HSAPHOL_node_(—)35 (SEQ ID NO: 33) according to the present invention is supported by 51 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HSAPHOL_T4 (SEQ ID NO: 12), HSAPHOL_T5 (SEQ ID NO: 13), HSAPHOL_T6 (SEQ ID NO: 14) and HSAPHOL_T8 (SEQ ID NO: 16). Table 34 below describes the starting and ending position of this segment on each transcript.

TABLE 34 Segment location on transcripts Segment Segment Transcript starting ending name position position HSAPHOL_T4 1156 1221 (SEQ ID NO:12) HSAPHOL_T5 1201 1266 (SEQ ID NO:13) HSAPHOL_T6 1100 1165 (SEQ ID NO:14) HSAPHOL_T8 1186 1251 (SEQ ID NO:16)

Segment cluster HSAPHOL_node_(—)36 (SEQ ID NO: 34) according to the present invention is supported by 47 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HSAPHOL_T4 (SEQ ID NO: 12), HSAPHOL_T5 (SEQ ID NO: 13), HSAPHOL T6 (SEQ ID NO: 14), HSAPHOL T7 (SEQ ID NO: 15) and HSAPHOL_T8 (SEQ ID NO: 16). Table 35 below describes the starting and ending position of this segment on each transcript.

TABLE 35 Segment location on transcripts Segment Segment Transcript starting ending name position position HSAPHOL_T4 1222 1276 (SEQ ID NO:12) HSAPHOL_T5 1267 1321 (SEQ ID NO:13) HSAPHOL_T6 1166 1220 (SEQ ID NO:14) HSAPHOL_T7 1321 1375 (SEQ ID NO:15) HSAPHOL_T8 1252 1306 (SEQ ID NO:16)

Segment cluster HSAPHOL_node_(—)41 (SEQ ID NO: 35) according to the present invention is supported by 60 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HSAPHOL_T4 (SEQ ID NO: 12), HSAPHOL_T5 (SEQ ID NO: 13), HSAPHOL_T6 (SEQ ID NO: 14), HSAPHOL_T7 (SEQ ID NO: 15) and HSAPHOL_T8 (SEQ ID NO: 16). Table 36 below describes the starting and ending position of this segment on each transcript.

TABLE 36 Segment location on transcripts Segment Segment Transcript starting ending name position position HSAPHOL_T4 1760 1869 (SEQ ID NO:12) HSAPHOL_T5 1805 1914 (SEQ ID NO:13) HSAPHOL_T6 1704 1813 (SEQ ID NO:14) HSAPHOL_T7 1859 1968 (SEQ ID NO:15) HSAPHOL_T8 1790 1899 (SEQ ID NO:16)

Microarray (chip) data is also available for this gene as follows. As described above with regard to the cluster itself, various oligonucleotides were tested for being differentially expressed in various disease conditions, particularly cancer. The following oligonucleotides were found to hit this segment (with regard to ovarian cancer), shown in Table 37.

TABLE 37 Oligonucleotides related to this gene Oligonucleotide Overexpressed Chip name in cancers reference HSAPHOL_0_11_0 Ovarian cancer Ovary (SEQ ID NO:1012) Variant protein alignment to the previously known protein: Sequence name: /tmp/rTOip7OHMr/xEFXPsrVLD:PPBT_HUMAN Sequence documentation:

Alignment of: HSAPHOL_P2 (SEQ ID NO: 37) x PPBT_HUMAN ..

Alignment segment 1/1:

Quality: 4926.00 Escore: 0 Matching length: 507 Total length: 507 Matching Percent Similarity: 99.61 Matching Percent Identity: 99.41 Total Percent Similarity: 99.61 Total Percent Identity: 99.41 Gaps: 0

Alignment:

Sequence name: /tmp/rTOip7OHMr/xEFXPsrVLD:AAH21289 Sequence documentation:

Alignment of: HSAPHOL_P2 (SEQ ID NO: 37) x AAH21289 ..

Alignment segment 1/1:

Quality: 5108.00 Escore: 0 Matching length: 531 Total length: 586 Matching Percent Similarity: 100.00 Matching Percent Identity: 100.00 Total Percent Similarity: 90.61 Total Percent Identity: 90.61 Gaps: 1

Alignment:

Sequence name: /tmp/pYLJnulFqm/UcqrrsA3UA:PPBT_HUMAN Sequence documentation:

Alignment of: HSAPHOL_P3 (SEQ ID NO: 38) x PPBT_HUMAN ..

Alignment segment 1/1:

Quality: 4615.00 Escore: 0 Matching length: 484 Total length: 524 Matching Percent Similarity: 100.00 Matching Percent Identity: 99.79 Total Percent Similarity: 92.37 Total Percent Identity: 92.18 Gaps: 1

Alignment:

Sequence name: /tmp/pYLJnulFqm/UcqrrsA3UA:AAH21289 Sequence documentation:

Alignment of: HSAPHOL_P3 (SEQ ID NO: 38) x AAH21289 ..

Alignment segment 1/1:

Quality: 4626.00 Escore: 0 Matching length: 484 Total length: 524 Matching Percent Similarity: 100.00 Matching Percent Identity: 100.00 Total Percent Similarity: 92.37 Total Percent Identity: 92.37 Gaps: 1

Alignment:

Sequence name: /tmp/iYbOicGuUc/lMWHKKvS1d:PPBT_HUMAN Sequence documentation:

Alignment of: HSAPHOL_P4 (SEQ ID NO: 39) x PPBT_HUMAN ..

Alignment segment 1/1:

Quality: 4517.00 Escore: 0 Matching length: 463 Total length: 463 Matching Percent Similarity: 100.00 Matching Percent Identity: 99.78 Total Percent Similarity: 100.00 Total Percent Identity: 99.78 Gaps: 0

Alignment:

Sequence name: /tmp/iYbOicGuUc/lMWHKKvS1d:AAH21289 Sequence documentation:

Alignment of: HSAPHOL_P4 (SEQ ID NO: 39) x AAH21289 ..

Alignment segment 1/1:

Quality: 4528.00 Escore: 0 Matching length: 463 Total length: 463 Matching Percent Similarity: 100.00 Matching Percent Identity: 100.00 Total Percent Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0

Alignment:

Sequence name: /tmp/v0YiupJ4xl/W6HH5Tm6Ym:PPBT_HUMAN Sequence documentation:

Alignment of: HSAPHOL_P5 (SEQ ID NO: 40) x PPBT_HUMAN ..

Alignment segment 1/1:

Quality: 4816.00 Escore: 0 Matching length: 502 Total length: 524 Matching Percent Similarity: 100.00 Matching Percent Identity: 99.80 Total Percent Similarity: 95.80 Total Percent Identity: 95.61 Gaps: 1

Alignment:

Sequence name: /tmp/v0YiupJ4xl/W6HH5Tm6Ym:AAH21289 Sequence documentation:

Alignment of: HSAPHOL_P5 (SEQ ID NO: 40) x AAH21289 ..

Alignment segment 1/1:

Quality: 4827.00 Escore: 0 Matching length: 502 Total length: 524 Matching Percent Similarity: 100.00 Matching Percent Identity: 100.00 Total Percent Similarity: 95.80 Total Percent Identity: 95.80 Gaps: 1

Alignment:

Sequence name: /tmp/L1ylq0ddii/lFFtdNNCUx:PPBT_HUMAN Sequence documentation:

Alignment of: HSAPHOL_P6 (SEQ ID NO: 41) x PPBT_HUMAN ..

Alignment segment 1/1:

Quality: 4575.00 Escore: 0 Matching length: 479 Total length: 524 Matching Percent Similarity: 100.00 Matching Percent Identity: 99.79 Total Percent Similarity: 91.41 Total Percent Identity: 91.22 Gaps: 1

Alignment:

Sequence name: /tmp/L1ylq0ddii/lFFtdNNCUx:AAH21289 Sequence documentation:

Alignment of: HSAPHOL_P6 (SEQ ID NO: 41) x AAH21289 ..

Alignment segment 1/1:

Quality: 4586.00 Escore: 0 Matching length: 479 Total length: 524 Matching Percent Similarity: 100.00 Matching Percent Identity: 100.00 Total Percent Similarity: 91.41 Total Percent Identity: 91.41 Gaps: 1

Alignment:

Sequence name: /tmp/K05Xam2Hdo/CV0GTdjKcW:PPBT_HUMAN Sequence documentation:

Alignment of: HSAPHOL_P7 (SEQ ID NO: 42) x PPBT_HUMAN ..

Alignment segment 1/1:

Quality: 2574.00 Escore: 0 Matching length: 264 Total length: 264 Matching Percent Similarity: 100.00 Matching Percent Identity: 99.62 Total Percent Similarity: 100.00 Total Percent Identity: 99.62 Gaps: 0

Alignment:

Sequence name: /tmp/K05Xam2Hdo/CV0GTdjKcW:AAH21289 Sequence documentation:

Alignment of: HSAPHOL_P7 (SEQ ID NO: 42) x AAH21289 ..

Alignment segment 1/1:

Quality: 2585.00 Escore: 0 Matching length: 264 Total length: 264 Matching Percent Similarity: 100.00 Matching Percent Identity: 100.00 Total Percent Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0

Alignment:

Sequence name: /tmp/K05Xam2Hdo/CV0GTdjKcW:O75090 (SEQ ID NO: 958) Sequence documentation:

Alignment of: HSAPHOL_P7 (SEQ ID NO: 42) x O75090 (SEQ ID NO: 958) ..

Alignment segment 1/1:

Quality: 2585.00 Escore: 0 Matching length: 264 Total length: 264 Matching Percent Similarity: 100.00 Matching Percent Identity: 100.00 Total Percent Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0

Alignment:

Sequence name: /tmp/H6G7vkGMmy/rS1jwUOCll:PPBT_HUMAN Sequence documentation:

Alignment of: HSAPHOL_P8 (SEQ ID NO: 43) x PPBT_HUMAN ..

Alignment segment 1/1:

Quality: 2819.00 Escore: 0 Matching length: 288 Total length: 288 Matching Percent Similarity: 100.00 Matching Percent Identity: 99.65 Total Percent Similarity: 100.00 Total Percent Identity: 99.65 Gaps: 0

Alignment:

Sequence name: /tmp/H6G7vkGMmy/rS1jwUOCll:AAH21289 Sequence documentation:

Alignment of: HSAPHOL_P8 (SEQ ID NO: 43) x AAH21289 ..

Alignment segment 1/1:

Quality: 2830.00 Escore: 0 Matching length: 288 Total length: 288 Matching Percent Similarity: 100.00 Matching Percent Identity: 100.00 Total Percent Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0

Alignment:

Sequence name: /tmp/H6G7vkGMmy/rS1jwUOCll:O75090 (SEQ ID NO: 958) Sequence documentation:

Alignment of: HSAPHOL_P8 (SEQ ID NO: 43) x O75090 (SEQ ID NO: 958) ..

Alignment segment 1/1:

Quality: 2830.00 Escore: 0 Matching length: 288 Total length: 288 Matching Percent Similarity: 100.00 Matching Percent Identity: 100.00 Total Percent Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0

Alignment:

Description for Cluster T10888

Cluster T10888 features 4 transcript(s) and 8 segment(s) of interest, the names for which are given in Tables 1 and 2, respectively, the sequences themselves are given at the end of the application. The selected protein variants are given in table 3.

TABLE 1 Transcripts of interest Transcript Name SEQ ID NO: T10888_PEA_1_T1 44 T10888_PEA_1_T4 45 T10888_PEA_1_T5 46 T10888_PEA_1_T6 47

TABLE 2 Segments of interest Segment Name SEQ ID NO: T10888_PEA_1_node_11 48 T10888_PEA_1_node_12 49 T10888_PEA_1_node_17 50 T10888_PEA_1_node_4 51 T10888_PEA_1_node_6 52 T10888_PEA_1_node_7 53 T10888_PEA_1_node_9 54 T10888_PEA_1_node_15 55

TABLE 3 Proteins of interest Protein Name SEQ ID NO: T10888_PEA_1_P2 57 T10888_PEA_1_P4 58 T10888_PEA_1_P5 59 T10888_PEA_1_P6 60

These sequences are variants of the known protein Carcinoembryonic antigen-related cell adhesion molecule 6 precursor (SwissProt accession identifier CEA6_HUMAN; known also according to the synonyms Normal cross-reacting antigen; Nonspecific crossreacting antigen; CD66c antigen), SEQ ID NO:56, referred to herein as the previously known protein.

The sequence for protein Carcinoembryonic antigen-related cell adhesion molecule 6 precursor is given at the end of the application, as “Carcinoembryonic antigen-related cell adhesion molecule 6 precursor amino acid sequence”. Known polymorphisms for this sequence are as shown in Table 4.

TABLE 4 Amino acid mutations for Known Protein SNP position(s) on amino acid sequence Comment 138 F -> L 239 V -> G

Protein Carcinoembryonic antigen-related cell adhesion molecule 6 precursor localization is believed to be attached to the membrane by a GPI-anchor.

The previously known protein also has the following indication(s) and/or potential therapeutic use(s): Cancer. It has been investigated for clinical/therapeutic use in humans, for example as a target for an antibody or small molecule, and/or as a direct therapeutic; available information related to these investigations is as follows. Potential pharmaceutically related or therapeutically related activity or activities of the previously known protein are as follows: Immunostimulant. A therapeutic role for a protein represented by the cluster has been predicted. The cluster was assigned this field because there was information in the drug database or the public databases (e.g., described herein above) that this protein, or part thereof, is used or can be used for a potential therapeutic indication: Imaging agent; Anticancer; Immunostimulant; Immunoconjugate; Monoclonal antibody, murine; Antisense therapy; antibody.

The following GO Annotation(s) apply to the previously known protein. The following annotation(s) were found: signal transduction; cell-cell signaling, which are annotation(s) related to Biological Process; and integral plasma membrane protein, which are annotation(s) related to Cellular Component.

The GO assignment relies on information from one or more of the SwissProt/TremBl Protein knowledgebase, available from <http://www.expasy.ch/sprot/>; or Locuslink, available from <http://www.ncbi.nlm.nih.gov/projects/LocusLink/>.

Cluster T10888 can be used as a diagnostic marker according to overexpression of transcripts of this cluster in cancer. Expression of such transcripts in normal tissues is also given according to the previously described methods. The term “number” in the right hand column of the table and the numbers on the y-axis of FIG. 9 refer to weighted expression of ESTs in each category, as “parts per million” (ratio of the expression of ESTs for a particular cluster to the expression of all ESTs in that category, according to parts per million).

Overall, the following results were obtained as shown with regard to the histograms in FIG. 9 and Table 5. This cluster is overexpressed (at least at a minimum level) in the following pathological conditions: colorectal cancer, a mixture of malignant tumors from different tissues, pancreas carcinoma and gastric carcinoma.

TABLE 5 Normal tissue distribution Name of Tissue Number bladder 0 colon 107 epithelial 52 general 22 head and neck 40 lung 237 breast 0 pancreas 32 prostate 12 stomach 0

TABLE 6 P values and ratios for expression in cancerous tissue Name of Tissue P1 P2 SP1 R3 SP2 R4 bladder 5.4e−01 3.4e−01 5.6e−01 1.8 4.6e−01 1.9 colon 1.2e−01 1.7e−01 2.8e−05 3.7 7.9e−04 2.8 epithelial 3.3e−02 2.1e−01 2.8e−20 2.8 4.8e−10 1.9 general 3.3e−05 2.2e−03 1.9e−44 4.9 4.6e−27 3.3 head and neck 4.6e−01 4.3e−01 1 0.8 7.5e−01 1.0 lung 7.6e−01 8.2e−01 8.9e−01 0.6 1 0.3 breast 3.7e−02 4.1e−02 1.5e−01 3.3 3.1e−01 2.4 pancreas 2.6e−01 2.4e−01 8.6e−23 2.8 1.5e−19 4.5 prostate 9.1e−01 9.3e−01 4.1e−02 1.2 1.0e−01 1.0 stomach 4.5e−02 5.6e−02 5.1e−04 4.1 4.7e−04 6.3

As noted above, cluster T10888 features 4 transcript(s), which were listed in Table 1 above. These transcript(s) encode for protein(s) which are variant(s) of protein Carcinoembryonic antigen-related cell adhesion molecule 6 precursor. A description of each variant protein according to the present invention is now provided.

Variant protein T10888_PEA_(—)1_P2 (SEQ ID NO: 57) according to the present invention has an amino acid sequence as given at the end of the application; it is encoded by transcript(s) T10888_PEA_(—)1_T1 (SEQ ID NO: 44). An alignment is given to the known protein (Carcinoembryonic antigen-related cell adhesion molecule 6 precursor) at the end of the application. One or more alignments to one or more previously published protein sequences are given at the end of the application. A brief description of the relationship of the variant protein according to the present invention to each such aligned protein is as follows:

Comparison report between T10888_PEA_(—)1_P2 (SEQ ID NO: 57) and CEA6_HUMAN:

1. An isolated chimeric polypeptide encoding for T10888_PEA_(—)1_P2 (SEQ ID NO: 57), comprising a first amino acid sequence being at least 90% homologous to MGPPSAPPCRLHVPWKEVLLTASLLTFWNPPTTAKLTIESTPFNVAEGKEVLLLAHNLP QNRIGYSWYKGERVDGNSLIVGYVIGTQQATPGPAYSGRETIYPNASLLIQNVTQNDTG FYTLQVIKSDLVNEEATGQFHVYPELPKPSISSNNSNPVEDKDAVAFTCEPEVQNTTYL WWVNGQSLPVSPRLQLSNGNMTLTLLSVKRNDAGSYECEIQNPASANRSDPVTLNVLY GPDVPTISPSKANYRPGENLNLSCHAASNPPAQYSWFINGTFQQSTQELFIPNITVNNSGS YMCQAHNSATGLNRTTVTMITVS corresponding to amino acids 1-319 of CEA6_HUMAN, which also corresponds to amino acids 1-319 of T10888_PEA_(—)1_P2 (SEQ ID NO: 57), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence DWTRP (SEQ ID NO: 1114) corresponding to amino acids 320-324 of T10888_PEA_(—)1_P2 (SEQ ID NO: 57), wherein said first and second amino acid sequences are contiguous and in a sequential order.

2. An isolated polypeptide encoding for a tail of T10888_PEA_(—)1_P2 (SEQ ID NO: 57), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence DWTRP (SEQ ID NO: 1114) in T10888_PEA_(—)1_P2 (SEQ ID NO: 57).

The location of the variant protein was determined according to results from a number of different software programs and analyses, including analyses from SignalP and other specialized programs. The variant protein is believed to be located as follows with regard to the cell: secreted. The protein localization is believed to be secreted because both signal-peptide prediction programs predict that this protein has a signal peptide, and neither trans-membrane region prediction program predicts that this protein has a trans-membrane region.

Variant protein T10888_PEA_(—)1_P2 (SEQ ID NO: 57) also has the following non-silent SNPs (Single Nucleotide Polymorphisms) as listed in Table 7, (given according to their position(s) on the amino acid sequence, with the alternative amino acid(s) listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein T10888_PEA_(—)1_P2 (SEQ ID NO: 57) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 7 Amino acid mutations SNP position(s) on amino acid sequence Alternative amino acid(s) Previously known SNP? 13 V -> No 232 N -> D No 324 P -> No 63 I -> No 92 G -> No

Variant protein T10888_PEA_(—)1_P2 (SEQ ID NO: 57) is encoded by the following transcript(s): T10888_PEA_(—)1_T1 (SEQ ID NO: 44), for which the sequence(s) is/are given at the end of the application. The coding portion of transcript T10888_PEA_(—)1_T1 (SEQ ID NO: 44) is shown in bold; this coding portion starts at position 151 and ends at position 1122. The transcript also has the following SNPs as listed in Table 8 (given according to their position on the nucleotide sequence, with the alternative nucleic acid listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein T10888_PEA_(—)1_P2 (SEQ ID NO: 57) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 8 Nucleic acid SNPs SNP position on nucleotide sequence Alternative nucleic acid Previously known SNP? 119 C -> T No 120 A -> T No 1062 A -> G Yes 1120 C -> No 1297 G -> T Yes 1501 A -> G Yes 1824 G -> A No 2036 A -> C No 2036 A -> G No 2095 A -> C No 2242 A -> C No 2245 A -> C No 189 C -> No 2250 A -> T Yes 2339 C -> A Yes 276 G -> A Yes 338 T -> No 424 G -> No 546 A -> G No 702 C -> T No 844 A -> G No 930 C -> T Yes

Variant protein T10888_PEA_(—)1_P4 (SEQ ID NO: 58) according to the present invention has an amino acid sequence as given at the end of the application; it is encoded by transcript(s) T10888_PEA_(—)1_T4 (SEQ ID NO: 45). An alignment is given to the known protein (Carcinoembryonic antigen-related cell adhesion molecule 6 precursor) at the end of the application. One or more alignments to one or more previously published protein sequences are given at the end of the application. A brief description of the relationship of the variant protein according to the present invention to each such aligned protein is as follows:

Comparison report between T10888_PEA_(—)1_P4 (SEQ ID NO: 58) and CEA6_HUMAN:

1. An isolated chimeric polypeptide encoding for T10888_PEA_(—)1_P4 (SEQ ID NO: 58), comprising a first amino acid sequence being at least 90% homologous to MGPPSAPPCRLHVPWKEVLLTASLLTFWNPPTTAKLTIESTPFNVAEGKEVLLLAHNLP QNRIGYSWYKGERVDGNSLIVGYVIGTQQATPGPAYSGRETIYPNASLLIQNVTQNDTG FYTLQVIKSDLVNEEATGQFHVYPELPKPSISSNNSNPVEDKDAVAFTCEPEVQNTTYL WWVNGQSLPVSPRLQLSNGNMTLTLLSVKRNDAGSYECEIQNPASANRSDPVTLNVL corresponding to amino acids 1-234 of CEA6_HUMAN, which also corresponds to amino acids 1-234 of T10888_PEA_(—)1_P4 (SEQ ID NO: 58), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence LLLSSQLWPPSASRLECWPGWL (SEQ ID NO: 1115) corresponding to amino acids 235-256 of T10888_PEA_(—)1_P4 (SEQ ID NO: 58), wherein said first and second amino acid sequences are contiguous and in a sequential order.

2. An isolated polypeptide encoding for a tail of T10888_PEA_(—)1_P4 (SEQ ID NO: 58), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence LLLSSQLWPPSASRLECWPGWL (SEQ ID NO: 1115) in T10888_PEA_(—)1_P4 (SEQ ID NO: 58).

Comparison report between T10888_PEA_(—)1_P4 (SEQ ID NO: 58) and Q13774 (SEQ ID NO: 959) (SEQ NO:959):

1. An isolated chimeric polypeptide encoding for T10888_PEA_(—)1_P4 (SEQ ID NO: 58), comprising a first amino acid sequence being at least 90% homologous to MGPPSAPPCRLHVPWKEVLLTASLLTFWNPPTTAKLTIESTPFNVAEGKEVLLLAHNLP QNRIGYSWYKGERVDGNSLIVGYVIGTQQATPGPAYSGRETIYPNASLLIQNVTQNDTG FYTLQVIKSDLVNEEATGQFHVYPELPKPSISSNNSNPVEDKDAVAFTCEPEVQNTTYL WWVNGQSLPVSPRLQLSNGNMTLTLLSVKRNDAGSYECEIQNPASANRSDPVTLNVL corresponding to amino acids 1-234 of Q13774 (SEQ ID NO: 959), which also corresponds to amino acids 1-234 of T10888_PEA_(—)1_P4 (SEQ ID NO: 58), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence LLLSSQLWPPSASRLECWPGWL (SEQ ID NO: 1115) corresponding to amino acids 235-256 of T10888_PEA_(—)1_P4 (SEQ ID NO: 58), wherein said first and second amino acid sequences are contiguous and in a sequential order.

2. An isolated polypeptide encoding for a tail of T10888_PEA_(—)1_P4 (SEQ ID NO: 58), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence LLLSSQLWPPSASRLECWPGWL (SEQ ID NO: 1115) in T10888_PEA_(—)1_P4 (SEQ ID NO: 58).

The location of the variant protein was determined according to results from a number of different software programs and analyses, including analyses from SignalP and other specialized programs. The variant protein is believed to be located as follows with regard to the cell: secreted. The protein localization is believed to be secreted because both signal-peptide prediction programs predict that this protein has a signal peptide, and neither trans-membrane region prediction program predicts that this protein has a trans-membrane region.

Variant protein T10888_PEA_(—)1_P4 (SEQ ID NO: 58) also has the following non-silent SNPs (Single Nucleotide Polymorphisms) as listed in Table 9, (given according to their position(s) on the amino acid sequence, with the alternative amino acid(s) listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein T10888_PEA_(—)1_P4 (SEQ ID NO: 58) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 9 Amino acid mutations SNP position(s) on amino acid sequence Alternative amino acid(s) Previously known SNP? 13 V -> No 232 N -> D No 63 I -> No 92 G -> No

Variant protein T10888_PEA_(—)1_P4 (SEQ ID NO: 58) is encoded by the following transcript(s): T10888_PEA_(—)1_T4 (SEQ ID NO: 45), for which the sequence(s) is/are given at the end of the application. The coding portion of transcript T10888_PEA_(—)1_T4 (SEQ ID NO: 45) is shown in bold; this coding portion starts at position 151 and ends at position 918′. The transcript also has the following SNPs as listed in Table 10 (given according to their position on the nucleotide sequence, with the alternative nucleic acid listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein T10888_PEA_(—)1_P4 (SEQ ID NO: 58) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 10 Nucleic acid SNPs SNP position on nucleotide sequence Alternative nucleic acid Previously known SNP? 119 C -> T No 120 A -> T No 978 C -> No 1155 G -> T Yes 1359 A -> G Yes 1682 G -> A No 1894 A -> C No 1894 A -> G No 1953 A -> C No 2100 A -> C No 2103 A -> C No 2108 A -> T Yes 189 C -> No 2197 C -> A Yes 276 G -> A Yes 338 T -> No 424 G -> No 546 A -> G No 702 C -> T No 844 A -> G No 958 G -> No

Variant protein T10888_PEA_(—)1_P5 (SEQ ID NO: 59) according to the present invention has an amino acid sequence as given at the end of the application; it is encoded by transcript(s) T10888_PEA_(—)1_T5 (SEQ ID NO: 46). An alignment is given to the known protein (Carcinoembryonic antigen-related cell adhesion molecule 6 precursor) at the end of the application. One or more alignments to one or more previously published protein sequences are given at the end of the application. A brief description of the relationship of the variant protein according to the present invention to each such aligned protein is as follows:

Comparison report between T10888_PEA_(—)1_P5 (SEQ ID NO: 59) and CEA6_HUMAN:

1. An isolated chimeric polypeptide encoding for T10888_PEA_(—)1_P5 (SEQ ID NO: 59), comprising a first amino acid sequence being at least 90% homologous to MGPPSAPPCRLHVPWKEVLLTASLLTFWNPPTTAKLTIESTPFNVAEGKEVLLLAHNLP QNRIGYSWYKGERVDGNSLIVGYVIGTQQATPGPAYSGRETIYPNASLLIQNVTQNDTG FYTLQVIKSDLVNEEATGQFHVYPELPKPSISSNNSNPVEDKDAVAFTCEPEVQNTTYL WWVNGQSLPVSPRLQLSNGNMTLTLLSVKRNDAGSYECEIQNPASANRSDPVTLNVLY GPDVPTISPSKANYRPGENLNLSCHAASNPPAQYSWFINGTFQQSTQELFIPNITVNNSGS YMCQAHNSATGLNRTTVTMITVSG corresponding to amino acids 1-320 of CEA6_HUMAN, which also corresponds to amino acids 1-320 of T10888_PEA_(—)1_P5 (SEQ ID NO: 59), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence KWIHEALASHFQVESGSQRRARKKFSFPTCVQGAHANPKFSPEPSQFTSADSFPLVFLFF VVFCFLISHV (SEQ ID NO: 1116) corresponding to amino acids 321-390 of T10888_PEA_(—)1_P5 (SEQ ID NO: 59), wherein said first and second amino acid sequences are contiguous and in a sequential order.

2. An isolated polypeptide encoding for a tail of T10888_PEA_(—)1_P5 (SEQ ID NO: 59), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence KWIHEALASHFQVESGSQRRARKKFSFPTCVQGAHANPKFSPEPSQFTSADSFPLVFLFF VVFCFLISHV (SEQ ID NO: 1116) in T10888_PEA_(—)1_P5 (SEQ ID NO: 59).

The location of the variant protein was determined according to results from a number of different software programs and analyses, including analyses from SignalP and other specialized programs. The variant protein is believed to be located as follows with regard to the cell: membrane. The protein localization is believed to be membrane because although both signal-peptide prediction programs agree that this protein has a signal peptide, both trans-membrane region prediction programs predict that this protein has a trans-membrane region downstream of this signal peptide.

Variant protein T10888_PEA_(—)1_P5 (SEQ ID NO: 59) also has the following non-silent SNPs (Single Nucleotide Polymorphisms) as listed in Table 11, (given according to their position(s) on the amino acid sequence, with the alternative amino acid(s) listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein T10888_PEA_(—)1_P5 (SEQ ID NO: 59) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 11 Amino acid mutations SNP position(s) on amino acid sequence Alternative amino acid(s) Previously known SNP? 13 V -> No 232 N -> D No 63 I -> No 92 G -> No

Variant protein T10888_PEA_(—)1_P5 (SEQ ID NO: 59) is encoded by the following transcript(s): T10888_PEA_(—)1_T5 (SEQ ID NO: 46), for which the sequence(s) is/are given at the end of the application. The coding portion of transcript T10888_PEA_(—)1_T5 (SEQ ID NO: 46) is shown in bold; this coding portion starts at position 151 and ends at position 1320. The transcript also has the following SNPs as listed in Table 12 (given according to their position on the nucleotide sequence, with the alternative nucleic acid listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein T10888_PEA_(—)1_P5 (SEQ ID NO: 59) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 12 Nucleic acid SNPs SNP position on nucleotide sequence Alternative nucleic acid Previously known SNP? 119 C -> T No 120 A -> T No 1062 A -> G Yes 1943 C -> A Yes 2609 C -> T Yes 2647 C -> G No 2701 C -> T Yes 2841 T -> C Yes 189 C -> No 276 G -> A Yes 338 T -> No 424 G -> No 546 A -> G No 702 C -> T No 844 A -> G No 930 C -> T Yes

Variant protein T10888_PEA_(—)1_P6 (SEQ ID NO: 60) according to the present invention has an amino acid sequence as given at the end of the application; it is encoded by transcript(s) T10888_PEA_(—)1_T6 (SEQ ID NO: 47). An alignment is given to the known protein (Carcinoembryonic antigen-related cell adhesion molecule 6 precursor) at the end of the application. One or more alignments to one or more previously published protein sequences are given at the end of the application.

Comparison report between T10888_PEA_(—)1_P6 (SEQ ID NO: 60) and CEA6_HUMAN:

1. An isolated chimeric polypeptide encoding for T10888_PEA_(—)1_P6 (SEQ ID NO: 60), comprising a first amino acid sequence being at least 90% homologous to MGPPSAPPCRLHVPWKEVLLTASLLTFWNPPTTAKLTIESTPFNVAEGKEVLLLA HNLPQNRIGYSWYKGERVDGNSLIVGYVIGTQQATPGPAYSGRETIYPNASLLIQNVTQ NDTGFYTLQVIKSDLVNEEATGQFHVY corresponding to amino acids 1-141 of CEA6_HUMAN, which also corresponds to amino acids 1-141 of T10888_PEA_(—)1_P6 (SEQ ID NO: 60), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence REYFHMTSGCWGSVLLPTYGIVRPGLCLWPSLHYILYQGLDI (SEQ ID NO: 1117)

corresponding to amino acids 142-183 of T10888_PEA_(—)1_P6 (SEQ ID NO: 60), wherein said first and second amino acid sequences are contiguous and in a sequential order.

2. An isolated polypeptide encoding for a tail of T10888_PEA_(—)1_P6 (SEQ ID NO: 60), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence REYFHMTSGCWGSVLLPTYGIVRPGLCLWPSLHYILYQGLDI (SEQ ID NO: 1117) in T10888_PEA_(—)1_P6 (SEQ ID NO: 60).

The location of the variant protein was determined according to results from a number of different software programs and analyses, including analyses from SignalP and other specialized programs. The variant protein is believed to be located as follows with regard to the cell: secreted. The protein localization is believed to be secreted because both signal-peptide prediction programs predict that this protein has a signal peptide, and neither trans-membrane region prediction program predicts that this protein has a trans-membrane region.

Variant protein T10888_PEA_(—)1_P6 (SEQ ID NO: 60) also has the following non-silent SNPs (Single Nucleotide Polymorphisms) as listed in Table 13, (given according to their position(s) on the amino acid sequence, with the alternative amino acid(s) listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein T10888_PEA_(—)1_P6 (SEQ ID NO: 60) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 13 Amino acid mutations SNP position(s) on amino acid sequence Alternative amino acid(s) Previously known SNP? 13 V -> No 63 I -> No 92 G -> No

Variant protein T10888_PEA_(—)1_P6 (SEQ ID NO: 60) is encoded by the following transcript(s): T10888_PEA_(—)1_T6 (SEQ ID NO: 47), for which the sequence(s) is/are given at the end of the application. The coding portion of transcript T10888_PEA_(—)1_T6 (SEQ ID NO: 47) is shown in bold; this coding portion starts at position 151 and ends at position 699. The transcript also has the following SNPs as listed in Table 14 (given according to their position on the nucleotide sequence, with the alternative nucleic acid listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein T10888_PEA_(—)1_P6 (SEQ ID NO: 60) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 14 Nucleic acid SNPs SNP position on nucleotide sequence Alternative nucleic acid Previously known SNP? 119 C -> T No 120 A -> T No 189 C -> No 276 G -> A Yes 338 T -> No 424 G -> No 546 A -> G No

As noted above, cluster T10888 features 8 segment(s), which were listed in Table 2 above and for which the sequence(s) are given at the end of the application. These segment(s) are portions of nucleic acid sequence(s) which are described herein separately because they are of particular interest. A description of each segment according to the present invention is now provided.

Segment cluster T10888_PEA_(—)1_node_(—)11 (SEQ ID NO: 48) according to the present invention is supported by 57 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): T10888_PEA_(—)1_T1 (SEQ ID NO: 44) and T10888_PEA_(—)1_T5 (SEQ ID NO: 46). Table 15 below describes the starting and ending position of this segment on each transcript.

TABLE 15 Segment location on transcripts Segment Segment Transcript starting ending name position position T10888_PEA_1_T1 854 1108 (SEQ ID NO:44) T10888_PEA_1_T5 854 1108 (SEQ ID NO:46)

Segment cluster T10888_PEA_(—)1_node_(—)12 (SEQ ID NO: 49) according to the present invention is supported by 9 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): T10888_PEA_(—)1_T5 (SEQ ID NO: 46). Table 16 below describes the starting and ending position of this segment on each transcript.

TABLE 16 Segment location on transcripts Segment Segment Transcript starting ending name position position T10888_PEA_1_T5 1109 3004 (SEQ ID NO:46)

Segment cluster T10888_PEA_(—)1_node_(—)17 (SEQ ID NO: 50) according to the present invention is supported by 160 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): T10888_PEA_(—)1_T1 (SEQ ID NO: 44) and T10888_PEA_(—)1_T4 (SEQ ID NO: 45). Table 17 below describes the starting and ending position of this segment on each transcript.

TABLE 17 Segment location on transcripts Segment Segment Transcript starting ending name position position T10888_PEA_1_T1 1109 2518 (SEQ ID NO:44) T10888_PEA_1_T4 967 2376 (SEQ ID NO:45)

Segment cluster T10888_PEA_(—)1_node_(—)4 (SEQ ID NO: 51) according to the present invention is supported by 61 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): T10888_PEA_(—)1_T1 (SEQ ID NO: 44), T10888_PEA_(—)1_T4 (SEQ ID NO: 45), T10888_PEA_(—)1_T5 (SEQ ID NO: 46) and T10888_PEA_(—)1_T6 (SEQ ID NO: 47). Table 18 below describes the starting and ending position of this segment on each transcript.

TABLE 18 Segment location on transcripts Segment Segment Transcript starting ending name position position T10888_PEA_1_T1 1 214 (SEQ ID NO:44) T10888_PEA_1_T4 1 214 (SEQ ID NO:45) T10888_PEA_1_T5 1 214 (SEQ ID NO:46) T10888_PEA_1_T6 1 214 (SEQ ID NO:47)

Segment cluster T10888_PEA_(—)1_node_(—)6 (SEQ ID NO: 52) according to the present invention is supported by 81 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): T10888_PEA_(—)1_T1 (SEQ ID NO: 44), T10888_PEA_(—)1_T4 (SEQ ID NO: 45), T10888_PEA_(—)1_T5 (SEQ ID NO: 46) and T10888_PEA_(—)1_T6 (SEQ ID NO: 47). Table 19 below describes the starting and ending position of this segment on each transcript.

TABLE 19 Segment location on transcripts Segment Segment Transcript name starting position ending position T10888_PEA_1_T1 215 574 (SEQ ID NO: 44) T10888_PEA_1_T4 215 574 (SEQ ID NO: 45) T10888_PEA_1_T5 215 574 (SEQ ID NO: 46) T10888_PEA_1_T6 215 574 (SEQ ID NO: 47)

Segment cluster T10888_PEA_(—)1_node_(—)7 (SEQ ID NO: 53) according to the present invention is supported by 4 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): T10888_PEA_(—)1_T6 (SEQ ID NO: 47). Table 20 below describes the starting and ending position of this segment on each transcript.

TABLE 20 Segment location on transcripts Segment Segment Transcript starting ending name position position T10888_PEA_1_T6 575 1410 (SEQ ID NO:47)

Segment cluster T10888_PEA_(—)1_node_(—)9 (SEQ ID NO: 54) according to the present invention is supported by 72 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): T10888_PEA_(—)1_T1 (SEQ ID NO: 44), T10888_PEA_(—)1_T4 (SEQ ID NO: 45) and T10888_PEA_(—)1_T5 (SEQ ID NO: 46). Table 21 below describes the starting and ending position of this segment on each transcript.

TABLE 21 Segment location on transcripts Segment Segment Transcript starting ending name position position T10888_PEA_1_T1 575 853 (SEQ ID NO:44) T10888_PEA_1_T4 575 853 (SEQ ID NO:45) T10888_PEA_1_T5 575 853 (SEQ ID NO:46)

According to an optional embodiment of the present invention, short segments related to the above cluster are also provided. These segments are up to about 120 bp in length, and so are included in a separate description.

Segment cluster T10888_PEA_(—)1_node_(—)15 (SEQ ID NO: 55) according to the present invention is supported by 39 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): T10888_PEA_(—)1_T4 (SEQ ID NO: 45). Table 22 below describes the starting and ending position of this segment on each transcript.

TABLE 22 Segment location on transcripts Segment Segment Transcript starting ending name position position T10888_PEA_1_T4 854 966 (SEQ ID NO:45) Variant protein alignment to the previously known protein: Sequence name: /tmp/tM4EgaoKvm/vuztUrlRc7:CEA6_HUMAN Sequence documentation:

Alignment of: T10888_PEA_(—)1_P2 (SEQ ID NO: 57) x CEA6_HUMAN ..

Alignment segment 1/1:

Quality: 3163.00 Escore: 0 Matching length: 319 Total length: 319 Matching Percent Similarity: 100.00 Matching Percent Identity: 100.00 Total Percent Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0

Alignment:

Sequence name: /tmp/Yjl1gj7TCe/PgdufzLOlW:CEA6_HUMAN Sequence documentation:

Alignment of: T10888_PEA_(—)1_P4 (SEQ ID NO: 58) x CEA6_HUMAN ..

Alignment segment 1/1:

Quality: 2310.00 Escore: 0 Matching length: 234 Total length: 234 Matching Percent Similarity: 100.00 Matching Percent Identity: 100.00 Total Percent Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0

Alignment:

Sequence name: /tmp/Yjl1gj7TCe/PgdufzLOlW:Q13774 (SEQ ID NO: 959) Sequence documentation:

Alignment of: T10888_PEA_(—)1_P4 (SEQ ID NO: 58) x Q13774 (SEQ ID NO: 959) ..

Alignment segment 1/1:

Quality: 2310.00 Escore: 0 Matching length: 234 Total length: 234 Matching Percent Similarity: 100.00 Matching Percent Identity: 100.00 Total Percent Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0

Alignment:

Sequence name: /tmp/x5xDBacdpj/rTXRGepv3y:CEA6_HUMAN Sequence documentation:

Alignment of: T10888_PEA_(—)1_P5 (SEQ ID NO: 59) x CEA6_HUMAN ..

Alignment segment 1/1:

Quality: 3172.00 Escore: 0 Matching length: 320 Total length: 320 Matching Percent Similarity: 100.00 Matching Percent Identity: 100.00 Total Percent Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0

Alignment:

Sequence name: /tmp/VAhvYFeatq/QNEM573uCo:CEA6_HUMAN Sequence documentation:

Alignment of: T10888_PEA_(—)1_P6 (SEQ ID NO: 60) x CEA6_HUMAN ..

Alignment segment 1/1:

Quality: 1393.00 Escore: 0 Matching length: 143 Total length: 143 Matching Percent Similarity: 99.30 Matching Percent Identity: 99.30 Total Percent Similarity: 99.30 Total Percent Identity: 99.30 Gaps: 0

Alignment:

Alignment of: T10888_PEA_(—)1_P6 (SEQ ID NO: 60) x CEA6_HUMAN ..

Alignment segment 1/1:

Quality: 101.00 Escore: 0 Matching length: 141 Total length: 183 Matching Percent Similarity: 100.00 Matching Percent Identity: 100.00 Total Percent Similarity: 77.05 Total Percent Identity: 77.05 Gaps: 1

Alignment:

Expression of CEA6_HUMAN Carcinoembryonic antigen-related cell adhesion molecule 6 T

10888 transcripts which are detectable by amplicon as depicted in sequence name T10888 junc11-17 (SEQ ID NO:962) in normal and cancerous ovary tissues

Expression of CEA6_HUMAN Carcinoembryonic antigen-related cell adhesion molecule 6 transcripts detectable by or according to junc11-17, T10888junc11-17 amplicon(s) (SEQ ID NO:962) and T10888junc11-17F (SEQ ID NO:960) and T10888junc11-17R (SEQ ID NO:961) primers was measured by real time PCR. In parallel the expression of four housekeeping genes —PBGD (GenBank Accession No. BC019323, (SEQ ID NO:1036); amplicon—PBGD-amplicon, (SEQ ID NO:1039)), HPRT1 (GenBank Accession No. NM_(—)000194, (SEQ ID NO:1040); amplicon—HPRT1-amplicon, (SEQ ID NO:1043)), and SDHA (GenBank Accession No. NM_(—)004168, (SEQ ID NO:1032); amplicon—SDHA-amplicon, (SEQ ID NO:1035)), GAPDH (GenBank Accession No. BC026907, (SEQ ID NO: 1044); GAPDH amplicon, (SEQ ID NO: 1047)) was measured similarly. For each RT sample, the expression of the above amplicon was normalized to the geometric mean of the quantities of the housekeeping genes. The normalized quantity of each RT sample was then divided by the median of the quantities of the normal post-mortem (PM) samples (Sample Nos. 45-48, 71, Table 1, “Tissue samples in testing panel”, above), to obtain a value of fold up-regulation for each sample relative to median of the normal PM samples.

FIG. 10 is a histogram showing over expression of the above-indicated CEA6_HUMAN Carcinoembryonic antigen-related cell adhesion molecule 6 transcripts in cancerous ovary samples relative to the normal samples. Values represent the average of duplicate experiments. Error bars indicate the minimal and maximal values obtained. The number and percentage of samples that exhibit at least 20 fold over-expression, out of the total number of samples tested is indicated in the bottom.

As is evident from FIG. 10, the expression of CEA6_HUMAN Carcinoembryonic antigen-related cell adhesion molecule 6 transcripts detectable by the above amplicon(s) in cancer samples was significantly higher than in the non-cancerous samples (Sample Nos. 45-48, 71, Table 1, “Tissue samples in testing panel”) and including benign samples (samples No. 56-65). Notably an over-expression of at least 20 fold was found in 25 out of 43 adenocarcinoma samples.

Statistical analysis was applied to verify the significance of these results, as described below. The P value for the difference in the expression levels of CEA6_HUMAN Carcinoembryonic antigen-related cell adhesion molecule 6 transcripts detectable by the above amplicon(s) in ovary cancer samples versus the normal tissue samples was determined by T test as 3.79E-02.

Threshold of 20 fold overexpression was found to differentiate between cancer and normal samples with P value of 1.97E-02 as checked by exact fisher test. The above values demonstrate statistical significance of the results.

Primer pairs are also optionally and preferably encompassed within the present invention; for example, for the above experiment, the following primer pair was used as a non-limiting illustrative example only of a suitable primer pair: T10888junc11-17F (SEQ ID NO:960) forward primer; and T10888junc11-17R (SEQ ID NO:961) reverse primer.

The present invention also preferably encompasses any amplicon obtained through the use of any suitable primer pair; for example, for the above experiment, the following amplicon was obtained as a non-limiting illustrative example only of a suitable amplicon:

T10888junc11-17 (SEQ ID NO: 962) T10888junc11-17F (SEQ ID NO: 960) CCAGCAATCCACACAAGAGCT T10888junc11-17R (SEQ ID NO: 961) CAGGGTCTGGTCCAATCAGAG T10888junc11-17 (SEQ ID NO: 962) CCAGCAATCCACACAAGAGCTCTTTATCCCCAACATCACTGTGAATAATA GCGGATCCTATATGTGCCAAGCCCATAACTCAGCCACTGGCCTCAATAGG ACCACAGTCACGATGATCACAGTCTCTGATTGGACCAGACCCTG

Expression of CEA6_HUMAN Carcinoembryonic antigen-related cell adhesion molecule 6 T 10888 transcripts which are detectable by amplicon as depicted in sequence name T10888 junc11-17 (SEQ ID NO:962) in different normal tissues.

Expression of CEA6_HUMAN Carcinoembryonic antigen-related cell adhesion molecule 6 transcripts detectable by or according to T10888 junc11-17 amplicon(s) and T10888 junc11-17F and T10888 junc11-17R was measured by real time PCR. In parallel the expression of four housekeeping genes -RPL19 (GenBank Accession No. NM_(—)000981, (SEQ ID NO:1048); RPL19 amplicon, (SEQ ID NO:1051)), TATA box (GenBank Accession No. NM_(—)003194, (SEQ ID NO:1052); TATA amplicon, (SEQ ID NO:1055)), Ubiquitin (GenBank Accession No. BC000449, (SEQ ID NO: 1056); amplicon—Ubiquitin-amplicon, (SEQ ID NO:1059)) and SDHA (GenBank Accession No. NM_(—)004168, (SEQ ID NO:1032); amplicon—SDHA-amplicon, (SEQ ID NO:1035)) was measured similarly. For each RT sample, the expression of the above amplicon was normalized to the geometric mean of the quantities of the housekeeping genes. The normalized quantity of each RT sample was then divided by the median of the quantities of the ovary samples (Sample Nos. 18-20, Table 2 above, “Tissue samples in normal panel”) to obtain a value of relative expression of each sample relative to median of the ovary samples.

The results are described in FIG. 11, presenting the histogram showing the expression of T10888 transcripts which are detectable by amplicon as depicted in sequence name T10888 junc11-17 (SEQ ID NO:962), in different normal tissues. Amplicon and primers are as above.

Description for Cluster HSECADH

Cluster HSECADH features 4 transcript(s) and 30 segment(s) of interest, the names for which are given in Tables 1 and 2, respectively, the sequences themselves are given at the end of the application. The selected protein variants are given in table 3.

TABLE 1 Transcripts of interest Transcript Name SEQ ID NO: HSECADH_T11 61 HSECADH_T18 62 HSECADH_T19 63 HSECADH_T20 64

TABLE 2 Segments of interest Segment Name SEQ ID NO: HSECADH_node_0 65 HSECADH_node_14 66 HSECADH_node_15 67 HSECADH_node_21 68 HSECADH_node_22 69 HSECADH_node_25 70 HSECADH_node_26 71 HSECADH_node_48 72 HSECADH_node_52 73 HSECADH_node_53 74 HSECADH_node_54 75 HSECADH_node_57 76 HSECADH_node_60 77 HSECADH_node_62 78 HSECADH_node_63 79 HSECADH_node_7 80 HSECADH_node_1 81 HSECADH_node_11 82 HSECADH_node_12 83 HSECADH_node_17 84 HSECADH_node_18 85 HSECADH_node_19 86 HSECADH_node_3 87 HSECADH_node_42 88 HSECADH_node_45 89 HSECADH_node_46 90 HSECADH_node_55 91 HSECADH_node_56 92 HSECADH_node_58 93 HSECADH_node_59 94

TABLE 3 Proteins of interest Protein Name SEQ ID NO: HSECADH_P9 96 HSECADH_P13 97 HSECADH_P14 98 HSECADH_P15 99

These sequences are variants of the known protein Epithelial-cadherin precursor (SwissProt accession identifier CAD1_HUMAN; known also according to the synonyms E-cadherin; Uvomorulin; Cadherin-1; CAM 120/80), SEQ ID NO:95, referred to herein as the previously known protein.

The variant proteins according to the present invention are variants of a known diagnostic marker, called E-Cadherin.

Protein Epithelial-cadherin is known or believed to have the following function(s): Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. E-cadherin has a potent invasive suppressor role. It is also a ligand for integrin alpha-E/beta-7. The sequence for protein Epithelial-cadherin precursor is given at the end of the application, as “Epithelial-cadherin precursor amino acid sequence”. Known polymorphisms for this sequence are as shown in Table 4.

TABLE 4 Amino acid mutations for Known Protein SNP position(s) on amino acid sequence Comment 123 H -> Y (in diffuse gastric cancer). /FTId = VAR_001306. 193 T -> P (in diffuse gastric cancer). /FTId = VAR_001307. 418-423 Missing (in gastric carcinoma). /FTId = VAR_001313. 463 E -> Q (in diffuse gastric cancer). /FTId = VAR_001314. 470 T -> I. /FTId = VAR_001315. 473 V -> D (in diffuse gastric cancer). /FTId = VAR_001317. 487 V -> A (in HDGC). /FTId = VAR_008713. 592 A -> T (in thyroid cancer; may play a role in colorectal carcinogenesis). /FTId = VAR_001318. 598 R -> Q (in diffuse gastric cancer). /FTId = VAR_001319. 617 A -> T (in endometrial cancer; loss of heterozygosity). /FTId = VAR_001320. 711 L -> V (in endometrial cancer). /FTId = VAR_001321. 838 S -> G (in ovarian cancer; loss of heterozygosity). /FTId = VAR_001322. 244 D -> G (in HDGC). /FTId = VAR_008712.  10 A -> G 16-51 QVSSWLCQEPEPCHPGFDAESYTFTVPRRHLERGRV -> RSPLGSQERSPPPCLTRELHVHGAPAPPEKRPR 68-75 YFSLDTRF -> IFLTPIP  95-102 QIHFLVYA -> TDPFLGLR 483 A -> G 530 A -> R 543 S -> F 615 I -> H 634-636 ASA -> RVP 868 R -> P 270 S -> A (may contribute to prostate cancer). /FTId = VAR_013970. 882 D -> H 274-277 Missing (in gastric adenocarcinoma). /FTId = VAR_001308. 315 N -> S (in lobular breast carcinoma). /FTId = VAR_001309. 336 E -> D. /FTId = VAR_001310. 340 T -> A (in HDGC and colorectal cancer). /FTId = VAR_013971. 370 D -> A (in diffuse gastric cancer). /FTId = VAR_001311. 400 Missing (in gastric carcinoma; loss of heterozygosity). /FTId = VAR_001312.

Protein Epithelial-cadherin localization is believed to be Type I membrane protein.

The following GO Annotation(s) apply to the previously known protein. The following annotation(s) were found: cell adhesion; homophilic cell adhesion, which are annotation(s) related to Biological Process; calcium binding; protein binding, which are annotation(s) related to Molecular Function; and membrane; integral membrane protein, which are annotation(s) related to Cellular Component.

The GO assignment relies on information from one or more of the SwissProt/TremBl Protein knowledgebase, available from <http://www.expasy.ch/sprot/>; or Locuslink, available from <http://www.ncbi.nlm.nih.gov/projects/LocusLink/>.

Cluster HSECADH can be used as a diagnostic marker according to overexpression of transcripts of this cluster in cancer. Expression of such transcripts in normal tissues is also given according to the previously described methods. The term “number” in the right hand column of the table and the numbers on the y-axis of FIG. 12 refer to weighted expression of ESTs in each category, as “parts per million” (ratio of the expression of ESTs for a particular cluster to the expression of all ESTs in that category, according to parts per million).

Overall, the following results were obtained as shown with regard to the histograms in FIG. 12 and Table 5. This cluster is overexpressed (at least at a minimum level) in the following pathological conditions: a mixture of malignant tumors from different tissues and ovarian carcinoma.

TABLE 5 Normal tissue distribution Name of Tissue Number bladder 41 brain 3 colon 299 epithelial 190 general 67 head and neck 10 kidney 103 liver 9 lung 93 breast 52 ovary 0 pancreas 105 prostate 279 skin 457 stomach 659 Thyroid 64 uterus 118

TABLE 6 P values and ratios for expression in cancerous tissue Name of Tissue P1 P2 SP1 R3 SP2 R4 bladder 3.9e−01 3.4e−01 4.1e−01 1.7 3.8e−01 1.7 brain 3.7e−01 4.9e−01 1 1.4 1 1.0 colon 6.6e−01 7.4e−01 9.5e−01 0.6 9.3e−01 0.5 epithelial 1.3e−01 6.8e−01 9.5e−01 0.8 1 0.5 general 1.6e−06 1.5e−03 6.3e−05 1.5 5.6e−01 0.9 head and neck 1.5e−01 2.7e−01 4.6e−01 2.1 7.5e−01 1.2 kidney 8.3e−01 8.7e−01 9.9e−01 0.4 1 0.3 liver 4.4e−01 6.9e−01 1 1.7 6.9e−01 1.5 lung 7.2e−01 8.8e−01 7.5e−01 0.9 9.9e−01 0.4 breast 7.5e−02 1.1e−01 3.1e−01 1.7 5.1e−01 1.2 ovary 4.5e−02 3.6e−02 4.7e−03 3.8 1.4e−02 3.5 pancreas 5.5e−01 6.5e−01 2.4e−01 0.9 5.2e−01 0.7 prostate 8.1e−01 8.5e−01 6.4e−01 0.8 9.0e−01 0.6 skin 5.7e−01 7.4e−01 1 0.0 1 0.1 stomach 2.2e−01 5.2e−01 1 0.2 1 0.1 Thyroid 5.5e−01 5.5e−01 4.4e−01 1.6 4.4e−01 1.6 uterus 5.0e−02 2.4e−01 1.0e−01 1.3 5.8e−01 0.8

As noted above, cluster HSECADH features 4 transcript(s), which were listed in Table 1 above. These transcript(s) encode for protein(s) which are variant(s) of protein Epithelial-cadherin precursor. A description of each variant protein according to the present invention is now provided.

Variant protein HSECADH_P9 (SEQ ID NO: 96) according to the present invention has an amino acid sequence as given at the end of the application; it is encoded by transcript(s) HSECADH_T11 (SEQ ID NO: 61). An alignment is given to the known protein (Epithelial-cadherin precursor) at the end of the application. One or more alignments to one or more previously published protein sequences are given at the end of the application. A brief description of the relationship of the variant protein according to the present invention to each such aligned protein is as follows:

Comparison report between HSECADH_P9 (SEQ ID NO: 96) and Q9UII7 (SEQ ID NO: 963) (SEQ ID NO:963):

1. An isolated chimeric polypeptide encoding for HSECADH_P9 (SEQ ID NO: 96), comprising a first amino acid sequence being at least 90% homologous to MGPWSRSLSALLLLLQVSSWLCQEPEPCHPGFDAESYTFTVPRRHLERGRVLGRVNFED CTGRQRTAYFSLDTRFKVGTDGVITVKRPLRFHNPQIHFLVYAWDSTYRKFSTKVTLNT VGHHHRPPPHQASVSGIQAELLTFPNSSPGLRRQKRDWVIPPISCPENEKGPFPKNLVQI KSNKDKEGKVFYSITGQGADTPPVGVFIIERETGWLKVTEPLDRERIATYTLFSHAVSSN GNAVEDPMEILITVTDQNDNKPEFTQEVFKGSVMEG corresponding to amino acids 1-274 of Q9UII7 (SEQ ID NO: 963), which also corresponds to amino acids 1-274 of HSECADH_P9 (SEQ ID NO: 96), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence TACRSRIANSCHSGDSWRNSCFANSDSAALAVSSEESGGQRALTAPRG (SEQ ID NO: 1118) corresponding to amino acids 275-322 of HSECADH_P9 (SEQ ID NO: 96), wherein said first and second amino acid sequences are contiguous and in a sequential order.

2. An isolated polypeptide encoding for a tail of HSECADH_P9 (SEQ ID NO: 96), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence TACRSRIANSCHSGDSWRNSCFANSDSAALAVSSEESGGQRALTAPRG (SEQ ID NO: 1118) in HSECADH_P9 (SEQ ID NO: 96).

Comparison report between HSECADH_P9 (SEQ ID NO: 96) and Q9UII8 (SEQ ID NO: 964) (SEQ ID NO:964):

1. An isolated chimeric polypeptide encoding for HSECADH_P9 (SEQ ID NO: 96), comprising a first amino acid sequence being at least 90% homologous to MGPWSRSLSALLLLLQVSSWLCQEPEPCHPGFDAESYTFTVPRRHLERGRVLGRVNFED CTGRQRTAYFSLDTRFKVGTDGVITVKRPLRFHNPQIHFLVYAWDSTYRKFSTKVTLNT VGHHHRPPPHQASVSGIQAELLTFPNSSPGLRRQKRDWVIPPISCPENEKGPFPKNLVQI KSNKDKEGKVFYSITGQGADTPPVGVFIIERETGWLKVTEPLDRERIATYTLFSHAVSSN GNAVEDPMEILITVTDQNDNKPEFTQEVFKGSVMEG corresponding to amino acids 1-274 of Q9UII8 (SEQ ID NO: 964), which also corresponds to amino acids 1-274 of HSECADH_P9 (SEQ ID NO: 96), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence TACRSRIANSCHSGDSWRNSCFANSDSAALAVSSEESGGQRALTAPRG (SEQ ID NO: 1118) corresponding to amino acids 275-322 of HSECADH_P9 (SEQ ID NO: 96), wherein said first and second amino acid sequences are contiguous and in a sequential order.

2. An isolated polypeptide encoding for a tail of HSECADH_P9 (SEQ ID NO: 96), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence TACRSRIANSCHSGDSWRNSCFANSDSAALAVSSEESGGQRALTAPRG (SEQ ID NO: 1118) in HSECADH_P9 (SEQ ID NO: 96).

Comparison report between HSECADH_P9 (SEQ ID NO: 96) and CAD1_HUMAN:

1. An isolated chimeric polypeptide encoding for HSECADH_P9 (SEQ ID NO: 96), comprising a first amino acid sequence being at least 90% homologous to MGPWSRSLSALLLLLQVSSWLCQEPEPCHPGFDAESYTFTVPRRHLERGRVLGRVNFED CTGRQRTAYFSLDTRFKVGTDGVITVKRPLRFHNPQIHFLVYAWDSTYRKFSTKVTLNT VGHHHRPPPHQASVSGIQAELLTFPNSSPGLRRQKRDWVIPPISCPENEKGPFPKNLVQI KSNKDKEGKVFYSITGQGADTPPVGVFIIERETGWLKVTEPLDRERIATYTLFSHAVSSN GNAVEDPMEILITVTDQNDNKPEFTQEVFKGSVMEG corresponding to amino acids 1-274 of CAD1_HUMAN, which also corresponds to amino acids 1-274 of HSECADH_P9 (SEQ ID NO: 96), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence TACRSRIANSCHSGDSWRNSCFANSDSAALAVSSEESGGQRALTAPRG (SEQ ID NO: 1118) corresponding to amino acids 275-322 of HSECADH_P9 (SEQ ID NO: 96), wherein said first and second amino acid sequences are contiguous and in a sequential order.

2. An isolated polypeptide encoding for a tail of HSECADH_P9 (SEQ ID NO: 96), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence TACRSRIANSCHSGDSWRNSCFANSDSAALAVSSEESGGQRALTAPRG (SEQ ID NO: 1118) in HSECADH_P9 (SEQ ID NO: 96).

The location of the variant protein was determined according to results from a number of different software programs and analyses, including analyses from SignalP and other specialized programs. The variant protein is believed to be located as follows with regard to the cell: secreted. The protein localization is believed to be secreted because both signal-peptide prediction programs predict that this protein has a signal peptide, and neither trans-membrane region prediction program predicts that this protein has a trans-membrane region.

Variant protein HSECADH_P9 (SEQ ID NO: 96) also has the following non-silent SNPs (Single Nucleotide Polymorphisms) as listed in Table 7, (given according to their position(s) on the amino acid sequence, with the alternative amino acid(s) listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein HSECADH_P9 (SEQ ID NO: 96) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 7 Amino acid mutations SNP position(s) on amino acid sequence Alternative amino acid(s) Previously known SNP? 127 P -> T No 141 T -> A No 276 A -> V No

Variant protein HSECADH_P9 (SEQ ID NO: 96) is encoded by the following transcript(s): HSECADH_T11 (SEQ ID NO: 61), for which the sequence(s) is/are given at the end of the application. The coding portion of transcript HSECADH_T11 (SEQ ID NO: 61) is shown in bold; this coding portion starts at position 125 and ends at position 1090. The transcript also has the following SNPs as listed in Table 8 (given according to their position on the nucleotide sequence, with the alternative nucleic acid listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein HSECADH_P9 (SEQ ID NO: 96) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 8 Nucleic acid SNPs SNP position on nucleotide sequence Alternative nucleic acid Previously known SNP? 71 G -> C Yes 469 G -> A Yes 1487 C -> T Yes 1556 C -> A Yes 1556 C -> G Yes 1556 C -> T Yes 1603 G -> A Yes 1604 G -> A Yes 1688 A -> G Yes 1712 T -> No 1890 T -> G No 1895 T -> G No 503 C -> A No 2090 C -> T Yes 2621 T -> A Yes 2621 T -> C Yes 2621 T -> G Yes 2797 -> G No 2849 G -> A No 2992 A -> C No 3027 C -> G No 3029 C -> A No 3134 T -> No 545 A -> G No 3211 T -> No 3258 A -> G No 3336 T -> C Yes 847 A -> G No 951 C -> T No 1331 T -> C No 1377 G -> A No 1487 C -> A Yes 1487 C -> G Yes

Variant protein HSECADH_P13 (SEQ ID NO: 97) according to the present invention has an amino acid sequence as given at the end of the application; it is encoded by transcript(s) HSECADH_T18 (SEQ ID NO: 62). An alignment is given to the known protein (Epithelial-cadherin precursor) at the end of the application. One or more alignments to one or more previously published protein sequences are given at the end of the application. A brief description of the relationship of the variant protein according to the present invention to each such aligned protein is as follows:

Comparison report between HSECADH_P13 (SEQ ID NO: 97) and Q9UII7 (SEQ ID NO: 963):

1. An isolated chimeric polypeptide encoding for HSECADH_P13 (SEQ ID NO: 97), comprising a first amino acid sequence being at least 90% homologous to MGPWSRSLSALLLLLQVSSWLCQEPEPCHPGFDAESYTFTVPRRHLERGRVLGRVNFED CTGRQRTAYFSLDTRFKVGTDGVITVKRPLRFHNPQIHFLVYAWDSTYRKFSTKVTLNT VGHHHRPPPHQASVSGIQAELLTFPNSSPGLRRQKRDWVIPPISCPENEKGPFPKNLVQI KSNKDKEGKVFYSITGQGADTPPVGVFIIERETGWLKVTEPLDRERIATYTLFSHAVSSN GNAVEDPMEILITVTDQNDNKPEFTQEVFKGSVMEGALPGTSVMEVTATDADDDVNT YNAAIAYTILSQDPELPDKNMFTINPNTGVISVVTTGLDRESFPTYTLVVQAADLQGEGL STTATAVITVTDTNDNPPIFNPTT corresponding to amino acids 1-379 of Q9UII7 (SEQ ID NO: 963), which also corresponds to amino acids 1-379 of HSECADH_P13 (SEQ ID NO: 97), and a second amino acid sequence VIL corresponding to amino acids 380-382 of HSECADH_P13 (SEQ ID NO: 97), wherein said first and second amino acid sequences are contiguous and in a sequential order.

Comparison report between HSECADH_P13 (SEQ ID NO: 97) and Q9UII8 (SEQ ID NO: 964):

1. An isolated chimeric polypeptide encoding for HSECADH_P13 (SEQ ID NO: 97), comprising a first amino acid sequence being at least 90% homologous to MGPWSRSLSALLLLLQVSSWLCQEPEPCHPGFDAESYTFTVPRRHLERGRVLGRVNFED CTGRQRTAYFSLDTRFKVGTDGVITVKRPLRFHNPQIHFLVYAWDSTYRKFSTKVTLNT VGHHHRPPPHQASVSGIQAELLTFPNSSPGLRRQKRDWVIPPISCPENEKGPFPKNLVQI KSNKDKEGKVFYSITGQGADTPPVGVFIIERETGWLKVTEPLDRERIATYTLFSHAVSSN GNAVEDPMEILITVTDQNDNKPEFTQEVFKGSVMEGALPGTSVMEVTATDADDDVNT YNAAIAYTILSQDPELPDKNMFTINRNTGVISVVTTGLDRESFPTYTLVVQAADLQGEGL STTATAVITVTDTNDNPPIFNPTT corresponding to amino acids 1-379 of Q9UII8 (SEQ ID NO: 964), which also corresponds to amino acids 1-379 of HSECADH_P13 (SEQ ID NO: 97), and a second amino acid sequence VIL corresponding to amino acids 380-382 of HSECADH_P13 (SEQ ID NO: 97), wherein said first and second amino acid sequences are contiguous and in a sequential order.

Comparison report between HSECADH_P13 (SEQ ID NO: 97) and CAD1_HUMAN:

1. An isolated chimeric polypeptide encoding for HSECADH_P13 (SEQ ID NO: 97), comprising a first amino acid sequence being at least 90% homologous to MGPWSRSLSALLLLLQVSSWLCQEPEPCHPGFDAESYTFTVPRRHLERGRVLGRVNFED CTGRQRTAYFSLDTRFKVGTDGVITVKRPLRFHNPQIHFLVYAWDSTYRKFSTKVTLNT VGHHHRPPPHQASVSGIQAELLTFPNSSPGLRRQKRDWVIPPISCPENEKGPFPKNLVQI KSNKDKEGKVFYSITGQGADTPPVGVFIIERETGWLKVTEPLDRERIATYTLFSHAVSSN GNAVEDPMEILITVTDQNDNKPEFTQEVFKGSVMEGALPGTSVMEVTATDADDDVNT YNAAIAYTILSQDPELPDKNMFTINRNTGVISVVTTGLDRESFPTYTLVVQAADLQGEGL STTATAVITVTDTNDNPPIFNPTT corresponding to amino acids 1-379 of CAD1_HUMAN, which also corresponds to amino acids 1-379 of HSECADH_P13 (SEQ ID NO: 97), and a second amino acid sequence VIL corresponding to amino acids 380-382 of HSECADH_P13 (SEQ ID NO: 97), wherein said first and second amino acid sequences are contiguous and in a sequential order.

The location of the variant protein was determined according to results from a number of different software programs and analyses, including analyses from SignalP and other specialized programs. The variant protein is believed to be located as follows with regard to the cell: secreted. The protein localization is believed to be secreted because both signal-peptide prediction programs predict that this protein has a signal peptide, and neither trans-membrane region prediction program predicts that this protein has a trans-membrane region.

Variant protein HSECADH_P13 (SEQ ID NO: 97) also has the following non-silent SNPs (Single Nucleotide Polymorphisms) as listed in Table 9, (given according to their position(s) on the amino acid sequence, with the alternative amino acid(s) listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein HSECADH_P13 (SEQ ID NO: 97) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 9 Amino acid mutations SNP position(s) on amino acid sequence Alternative amino acid(s) Previously known SNP? 127 P -> T No 141 T -> A No

Variant protein HSECADH_P13 (SEQ ID NO: 97) is encoded by the following transcript(s): HSECADH_T18 (SEQ ID NO: 62), for which the sequence(s) is/are given at the end of the application. The coding portion of transcript HSECADH_T18 (SEQ ID NO: 62) is shown in bold; this coding portion starts at position 125 and ends at position 1270. The transcript also has the following SNPs as listed in Table 10 (given according to their position on the nucleotide sequence, with the alternative nucleic acid listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein HSECADH_P13 (SEQ ID NO: 97) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 10 Nucleic acid SNPs SNP position on nucleotide sequence Alternative nucleic acid Previously known SNP? 71 G -> C Yes 469 G -> A Yes 503 C -> A No 545 A -> G No 847 A -> G No 1545 A -> G Yes

Variant protein HSECADH_P14 (SEQ ID NO: 98) according to the present invention has an amino acid sequence as given at the end of the application; it is encoded by transcript(s) HSECADH_T19 (SEQ ID NO: 63). An alignment is given to the known protein (Epithelial-cadherin precursor) at the end of the application. One or more alignments to one or more previously published protein sequences are given at the end of the application. A brief description of the relationship of the variant protein according to the present invention to each such aligned protein is as follows:

Comparison report between HSECADH_P14 (SEQ ID NO: 98) and Q9UII7 (SEQ ID NO: 963):

1. An isolated chimeric polypeptide encoding for HSECADH_P14 (SEQ ID NO: 98), comprising a first amino acid sequence being at least 90% homologous to MGPWSRSLSALLLLLQVSSWLCQEPEPCHPGFDAESYTFTVPRRHLERGRVLGRVNFED CTGRQRTAYFSLDTRFKVGTDGVITVKRPLRFHNPQIHFLVYAWDSTYRKFSTKVTLNT VGHHHRPPPHQASVSGIQAELLTFPNSSPGLRRQKRDWVIPPISCPENEKGPFPKNLVQI KSNKDKEGKVFYSITGQGADTPPVGVFIIERETGWLKVTEPLDRERIATYTLFSHAVSSN GNAVEDPMEILITVTDQNDNKPEFTQEVFKGSVMEGALPGTSVMEVTATDADDDVNT YNAAIAYTILSQDPELPDKNMFTINRNTGVISVVTTGLDRE corresponding to amino acids 1-336 of Q9UII7 (SEQ ID NO: 963), which also corresponds to amino acids 1-336 of HSECADH_P14 (SEQ ID NO: 98), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence VRGQEDPEGVEDKCVLAQSRGQSKILLGQLSVNTVMV (SEQ ID NO: 1119) corresponding to amino acids 337-373 of HSECADH_P14 (SEQ ID NO: 98), wherein said first and second amino acid sequences are contiguous and in a sequential order.

2. An isolated polypeptide encoding for a tail of HSECADH_P14 (SEQ ID NO: 98), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence VRGQEDPEGVEDKCVLAQSRGQSKILLGQLSVNTVMV (SEQ ID NO: 1119) in HSECADH_P14 (SEQ ID NO: 98).

Comparison report between HSECADH_P14 (SEQ ID NO: 98) and Q9UII8 (SEQ ID NO: 964):

1. An isolated chimeric polypeptide encoding for HSECADH_P14 (SEQ ID NO: 98), comprising a first amino acid sequence being at least 90% homologous to MGPWSRSLSALLLLLQVSSWLCQEPEPCHPGFDAESYTFTVPRRHLERGRVLGRVNFED CTGRQRTAYFSLDTRFKVGTDGVITVKRPLRFHNPQIHFLVYAWDSTYRKFSTKVTLNT VGHHHRPPPHQASVSGIQAELLTFPNSSPGLRRQKRDWVIPPISCPENEKGPFPKNLVQI KSNKDKEGKVFYSITGQGADTPPVGVFIIERETGWLKVTEPLDRERIATYTLFSHAVSSN GNAVEDPMEILITVTDQNDNKPEFTQEVFKGSVMEGALPGTSVMEVTATDADDDVNT YNAAIAYTILSQDPELPDKNMFTINRNTGVISVVTTGLDRE corresponding to amino acids 1-336 of Q9UII8 (SEQ ID NO: 964), which also corresponds to amino acids 1-336 of HSECADH_P14 (SEQ ID NO: 98), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence VRGQEDPEGVEDKCVLAQSRGQSKILLGQLSVNTVMV (SEQ ID NO: 1119) corresponding to amino acids 337-373 of HSECADH_P14 (SEQ ID NO: 98), wherein said first and second amino acid sequences are contiguous and in a sequential order.

2. An isolated polypeptide encoding for a tail of HSECADH_P14 (SEQ ID NO: 98), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence VRGQEDPEGVEDKCVLAQSRGQSKILLGQLSVNTVMV (SEQ ID NO: 1119) in HSECADH_P14 (SEQ ID NO: 98).

Comparison report between HSECADH_P14 (SEQ ID NO: 98) and CAD1_HUMAN:

1. An isolated chimeric polypeptide encoding for HSECADH_P14 (SEQ ID NO: 98), comprising a first amino acid sequence being at least 90% homologous to MGPWSRSLSALLLLLQVSSWLCQEPEPCHPGFDAESYTFTVPRRHLERGRVLGRVNFED CTGRQRTAYFSLDTRFKVGTDGVITVKRPLRFHNPQIHFLVYAWDSTYRKFSTKVTLNT VGHHHRPPPHQASVSGIQAELLTFPNSSPGLRRQKRDWVIPPISCPENEKGPFPKNLVQI KSNKDKEGKVFYSITGQGADTPPVGVFIIERETGWLKVTEPLDRERIATYTLFSHAVSSN GNAVEDPMEILITVTDQNDNKPEFTQEVFKGSVMEGALPGTSVMEVTATDADDDVNT YNAAIAYTILSQDPELPDKNMFTINRNTGVISVVTTGLDRE corresponding to amino acids 1-336 of CAD1_HUMAN, which also corresponds to amino acids 1-336 of HSECADH_P14 (SEQ ID NO: 98), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence VRGQEDPEGVEDKCVLAQSRGQSKILLGQLSVNTVMV (SEQ ID NO: 1119) corresponding to amino acids 337-373 of HSECADH_P14 (SEQ ID NO: 98), wherein said first and second amino acid sequences are contiguous and in a sequential order.

2. An isolated polypeptide encoding for a tail of HSECADH_P14 (SEQ ID NO: 98), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence VRGQEDPEGVEDKCVLAQSRGQSKILLGQLSVNTVMV (SEQ ID NO: 1119) in HSECADH_P14 (SEQ ID NO: 98).

The location of the variant protein was determined according to results from a number of different software programs and analyses, including analyses from SignalP and other specialized programs. The variant protein is believed to be located as follows with regard to the cell: secreted. The protein localization is believed to be secreted because both signal-peptide prediction programs predict that this protein has a signal peptide, and neither trans-membrane region prediction program predicts that this protein has a trans-membrane region.

Variant protein HSECADH_P14 (SEQ ID NO: 98) also has the following non-silent SNPs (Single Nucleotide Polymorphisms) as listed in Table 11, (given according to their position(s) on the amino acid sequence, with the alternative amino acid(s) listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein HSECADH_P14 (SEQ ID NO: 98) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 11 Amino acid mutations SNP position(s) on amino acid sequence Alternative amino acid(s) Previously known SNP? 127 P -> T No 141 T -> A No

Variant protein HSECADH_P14 (SEQ ID NO: 98) is encoded by the following transcript(s): HSECADH_T19 (SEQ ID NO: 63), for which the sequence(s) is/are given at the end of the application. The coding portion of transcript HSECADH_T19 (SEQ ID NO: 63) is shown in bold; this coding portion starts at position 125 and ends at position 1243. The transcript also has the following SNPs as listed in Table 12 (given according to their position on the nucleotide sequence, with the alternative nucleic acid listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein HSECADH_P14 (SEQ ID NO: 98) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 12 Nucleic acid SNPs SNP position on nucleotide sequence Alternative nucleic acid Previously known SNP? 71 G -> C Yes 469 G -> A Yes 503 C -> A No 545 A -> G No 847 A -> G No

Variant protein HSECADH_P15 (SEQ ID NO: 99) according to the present invention has an amino acid sequence as given at the end of the application; it is encoded by transcript(s) HSECADH_T20 (SEQ ID NO: 64). An alignment is given to the known protein (Epithelial-cadherin precursor) at the end of the application. One or more alignments to one or more previously published protein sequences are given at the end of the application. A brief description of the relationship of the variant protein according to the present invention to each such aligned protein is as follows:

Comparison report between HSECADH_P15 (SEQ ID NO: 99) and Q9UII7 (SEQ ID NO: 963):

1. An isolated chimeric polypeptide encoding for HSECADH_P15 (SEQ ID NO: 99), comprising a first amino acid sequence being at least 90% homologous to MGPWSRSLSALLLLLQVSSWLCQEPEPCHPGFDAESYTFTVPRRHLERGRVLGRVNFED CTGRQRTAYFSLDTRFKVGTDGVITVKRPLRFHNPQIHFLVYAWDSTYRKFSTKVTLNT VGHHHRPPPHQASVSGIQAELLTFPNSSPGLRRQKRDWVIPPISCPENEKGPFPKNLVQI KSNKDKEGKVFYSITGQGADTPPVGVFIIERETGWLKVTEPLDRERIATYT corresponding to amino acids 1-229 of Q9UII7 (SEQ ID NO: 963), which also corresponds to amino acids 1-229 of HSECADH_P15 (SEQ ID NO: 99), and a second amino acid sequence VSIS corresponding to amino acids 230-233 of HSECADH_P15 (SEQ ID NO: 99), wherein said first and second amino acid sequences are contiguous and in a sequential order.

Comparison report between HSECADH_P15 (SEQ ID NO: 99) and Q9UII8 (SEQ ID NO: 964):

1. An isolated chimeric polypeptide encoding for HSECADH_P15 (SEQ ID NO: 99), comprising a first amino acid sequence being at least 90% homologous to MGPWSRSLSALLLLLQVSSWLCQEPEPCHPGFDAESYTFTVPRRHLERGRVLGRVNFED CTGRQRTAYFSLDTRFKVGTDGVITVKRPLRFHNPQIHFLVYAWDSTYRKFSTKVTLNT VGHHHRPPPHQASVSGIQAELLTFPNSSPGLRRQKRDWVIPPISCPENEKGPFPKNLVQI KSNKDKEGKVFYSITGQGADTPPVGVFIIERETGWLKVTEPLDRERIATYT corresponding to amino acids 1-229 of Q9UII8 (SEQ ID NO: 964), which also corresponds to amino acids 1-229 of HSECADH_P15 (SEQ ID NO: 99), and a second amino acid sequence VSIS corresponding to amino acids 230-233 of HSECADH_P15 (SEQ ID NO: 99), wherein said first and second amino acid sequences are contiguous and in a sequential order.

Comparison report between HSECADH_P15 (SEQ ID NO: 99) and CAD1_HUMAN:

1. An isolated chimeric polypeptide encoding for HSECADH_P15 (SEQ ID NO: 99), comprising a first amino acid sequence being at least 90% homologous to MGPWSRSLSALLLLLQVSSWLCQEPEPCHPGFDAESYTFTVPRRHLERGRVLGRVNFED CTGRQRTAYFSLDTRFKVGTDGVITVKRPLRFHNPQIHFLVYAWDSTYRKFSTKVTLNT VGHHHRPPPHQASVSGIQAELLTFPNSSPGLRRQKRDWVIPPISCPENEKGPFPKNLVQI KSNKDKEGKVFYSITGQGADTPPVGVFIIERETGWLKVTEPLDRERIATYT corresponding to amino acids 1-229 of CAD1_HUMAN, which also corresponds to amino acids 1-229 of HSECADH_P15 (SEQ ID NO: 99), and a second amino acid sequence VSIS corresponding to amino acids 230-233 of HSECADH_P15 (SEQ ID NO: 99), wherein said first and second amino acid sequences are contiguous and in a sequential order.

The location of the variant protein was determined according to results from a number of different software programs and analyses, including analyses from SignalP and other specialized programs. The variant protein is believed to be located as follows with regard to the cell: secreted. The protein localization is believed to be secreted because both signal-peptide prediction programs predict that this protein has a signal peptide, and neither trans-membrane region prediction program predicts that this protein has a trans-membrane region.

Variant protein HSECADH_P15 (SEQ ID NO: 99) also has the following non-silent SNPs (Single Nucleotide Polymorphisms) as listed in Table 13, (given according to their position(s) on the amino acid sequence, with the alternative amino acid(s) listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein HSECADH_P15 (SEQ ID NO: 99) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 13 Amino acid mutations SNP position(s) on amino acid sequence Alternative amino acid(s) Previously known SNP? 127 P -> T No 141 T -> A No

Variant protein HSECADH_P15 (SEQ ID NO: 99) is encoded by the following transcript(s): HSECADH_T20 (SEQ ID NO: 64), for which the sequence(s) is/are given at the end of the application. The coding portion of transcript HSECADH_T20 (SEQ ID NO: 64) is shown in bold; this coding portion starts at position 125 and ends at position 823. The transcript also has the following SNPs as listed in Table 14 (given according to their position on the nucleotide sequence, with the alternative nucleic acid listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein HSECADH_P15 (SEQ ID NO: 99) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 14 Nucleic acid SNPs SNP position on nucleotide sequence Alternative nucleic acid Previously known SNP? 71 G -> C Yes 469 G -> A Yes 503 C -> A No 545 A -> G No 955 G -> A Yes

As noted above, cluster HSECADH features 30 segment(s), which were listed in Table 2 above and for which the sequence(s) are given at the end of the application. These segment(s) are portions of nucleic acid sequence(s) which are described herein separately because they are of particular interest. A description of each segment according to the present invention is now provided.

Segment cluster HSECADH_node_(—)0 (SEQ ID NO: 65) according to the present invention is supported by 17 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HSECADH_T11 (SEQ ID NO: 61), HSECADH_T18 (SEQ ID NO: 62), HSECADH_T19 (SEQ ID NO: 63) and HSECADH_T20 (SEQ ID NO: 64). Table 15 below describes the starting and ending position of this segment on each transcript.

TABLE 15 Segment location on transcripts Segment Segment Transcript starting ending name position position HSECADH_T11 1 166 (SEQ ID NO:61) HSECADH_T18 1 166 (SEQ ID NO:62) HSECADH_T19 1 166 (SEQ ID NO:63) HSECADH_T20 1 166 (SEQ ID NO:64)

Segment cluster HSECADH_node_(—)14 (SEQ ID NO: 66) according to the present invention is supported by 40 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HSECADH_T11 (SEQ ID NO: 61), HSECADH_T18 (SEQ ID NO: 62), HSECADH_T19 (SEQ ID NO: 63) and HSECADH_T20 (SEQ ID NO: 64). Table 16 below describes the starting and ending position of this segment on each transcript.

TABLE 16 Segment location on transcripts Segment Segment Transcript starting ending name position position HSECADH_T11 656 811 (SEQ ID NO:61) HSECADH_T18 656 811 (SEQ ID NO:62) HSECADH_T19 656 811 (SEQ ID NO:63) HSECADH_T20 656 811 (SEQ ID NO:64)

Segment cluster HSECADH_node_(—)15 (SEQ ID NO: 67) according to the present invention is supported by 1 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HSECADH_T20 (SEQ ID NO: 64). Table 17 below describes the starting and ending position of this segment on each transcript.

TABLE 17 Segment location on transcripts Segment Segment Transcript starting ending name position position HSECADH_T20 812 970 (SEQ ID NO:64)

Segment cluster HSECADH_node_(—)21 (SEQ ID NO: 68) according to the present invention is supported by 40 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HSECADH_T18 (SEQ ID NO: 62) and HSECADH_T19 (SEQ ID NO: 63). Table 18 below describes the starting and ending position of this segment on each transcript.

TABLE 18 Segment location on transcripts Segment Segment Transcript starting ending name position position HSECADH_T18 957 1132 (SEQ ID NO:62) HSECADH_T19 957 1132 (SEQ ID NO:63)

Segment cluster HSECADH_node_(—)22 (SEQ ID NO: 69) according to the present invention is supported by 1 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HSECADH_T19 (SEQ ID NO: 63). Table 19 below describes the starting and ending position of this segment on each transcript.

TABLE 19 Segment location on transcripts Segment Segment Transcript starting ending name position position HSECADH_T19 1133 1269 (SEQ ID NO:63)

Segment cluster HSECADH_node_(—)25 (SEQ ID NO: 70) according to the present invention is supported by 34 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HSECADH_T18 (SEQ ID NO: 62). Table 20 below describes the starting and ending position of this segment on each transcript.

TABLE 20 Segment location on transcripts Segment Segment Transcript starting ending name position position HSECADH_T18 1133 1261 (SEQ ID NO:62)

Segment cluster HSECADH_node_(—)26 (SEQ ID NO: 71) according to the present invention is supported by 1 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HSECADH_T18 (SEQ ID NO: 62). Table 21 below describes the starting and ending position of this segment on each transcript.

TABLE 21 Segment location on transcripts Segment Segment Transcript starting ending name position position HSECADH_T18 1262 1584 (SEQ ID NO:62)

Segment cluster HSECADH_node_(—)48 (SEQ ID NO: 72) according to the present invention is supported by 44 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HSECADH_T11 (SEQ ID NO: 61). Table 22 below describes the starting and ending position of this segment on each transcript.

TABLE 22 Segment location on transcripts Segment Segment Transcript starting ending name position position HSECADH_T11 1149 1292 (SEQ ID NO:61)

Segment cluster HSECADH_node_(—)52 (SEQ ID NO: 73) according to the present invention is supported by 39 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HSECADH_T11 (SEQ ID NO: 61). Table 23 below describes the starting and ending position of this segment on each transcript.

TABLE 23 Segment location on transcripts Segment Segment Transcript starting ending name position position HSECADH_T11 1293 1449 (SEQ ID NO:61)

Segment cluster HSECADH_node_(—)53 (SEQ ID NO: 74) according to the present invention is supported by 59 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HSECADH_T11 (SEQ ID NO: 61). Table 24 below describes the starting and ending position of this segment on each transcript.

TABLE 24 Segment location on transcripts Segment Segment Transcript starting ending name position position HSECADH_T11 1450 1933 (SEQ ID NO:61)

Segment cluster HSECADH_node_(—)54 (SEQ ID NO: 75) according to the present invention is supported by 44 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HSECADH_T11 (SEQ ID NO: 61). Table 25 below describes the starting and ending position of this segment on each transcript.

TABLE 25 Segment location on transcripts Segment Segment Transcript starting ending name position position HSECADH_T11 1934 2053 (SEQ ID NO:61)

Segment cluster HSECADH_node_(—)57 (SEQ ID NO: 76) according to the present invention is supported by 67 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HSECADH_T11 (SEQ ID NO: 61). Table 26 below describes the starting and ending position of this segment on each transcript.

TABLE 26 Segment location on transcripts Segment Segment Transcript starting ending name position position HSECADH_T11 2241 2430 (SEQ ID NO:61)

Segment cluster HSECADH_node_(—)60 (SEQ ID NO: 77) according to the present invention is supported by 260 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HSECADH_T11 (SEQ ID NO: 61). Table 27 below describes the starting and ending position of this segment on each transcript.

TABLE 27 Segment location on transcripts Segment Segment Transcript starting ending name position position HSECADH_T11 2504 3096 (SEQ ID NO:61)

Segment cluster HSECADH_node_(—)62 (SEQ ID NO: 78) according to the present invention is supported by 173 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HSECADH_T11 (SEQ ID NO: 61). Table 28 below describes the starting and ending position of this segment on each transcript.

TABLE 28 Segment location on transcripts Segment Segment Transcript starting ending name position position HSECADH_T11 3097 3245 (SEQ ID NO:61)

Segment cluster HSECADH_node_(—)63 (SEQ ID NO: 79) according to the present invention is supported by 162 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HSECADH_T11 (SEQ ID NO: 61). Table 29 below describes the starting and ending position of this segment on each transcript.

TABLE 29 Segment location on transcripts Segment Segment Transcript starting ending name position position HSECADH_T11 3246 3544 (SEQ ID NO:61)

Segment cluster HSECADH_node_(—)7 (SEQ ID NO: 80) according to the present invention is supported by 21 libraries. The number of libraries was determined as previously described.

This segment can be found in the following transcript(s): HSECADH_T11 (SEQ ID NO: 61), HSECADH_T18 (SEQ ID NO: 62), HSECADH_T19 (SEQ ID NO: 63) and HSECADH_T20 (SEQ ID NO: 64). Table 30 below describes the starting and ending position of this segment on each transcript.

TABLE 30 Segment location on transcripts Segment Segment Transcript starting ending name position position HSECADH_T11 288 511 (SEQ ID NO:61) HSECADH_T18 288 511 (SEQ ID NO:62) HSECADH_T19 288 511 (SEQ ID NO:63) HSECADH_T20 288 511 (SEQ ID NO:64)

According to an optional embodiment of the present invention, short segments related to the above cluster are also provided. These segments are up to about 120 bp in length, and so are included in a separate description.

Segment cluster HSECADH_node_(—)1 (SEQ ID NO: 81) according to the present invention can be found in the following transcript(s): HSECADH_T11 (SEQ ID NO: 61), HSECADH_T18 (SEQ ID NO: 62), HSECADH_T19 (SEQ ID NO: 63) and HSECADH_T20 (SEQ ID NO: 64). Table 31 below describes the starting and ending position of this segment on each transcript.

TABLE 31 Segment location on transcripts Segment Segment Transcript starting ending name position position HSECADH_T11 167 172 (SEQ ID NO:61) HSECADH_T18 167 172 (SEQ ID NO:62) HSECADH_T19 167 172 (SEQ ID NO:63) HSECADH_T20 167 172 (SEQ ID NO:64)

Segment cluster HSECADH_node_(—)11 (SEQ ID NO: 82) according to the present invention is supported by 23 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HSECADH_T11 (SEQ ID NO: 61), HSECADH_T18 (SEQ ID NO: 62), HSECADH_T19 (SEQ ID NO: 63) and HSECADH_T20 (SEQ ID NO: 64). Table 32 below describes the starting and ending position of this segment on each transcript.

TABLE 32 Segment location on transcripts Segment Segment Transcript starting ending name position position HSECADH_T11 512 592 (SEQ ID NO:61) HSECADH_T18 512 592 (SEQ ID NO:62) HSECADH_T19 512 592 (SEQ ID NO:63) HSECADH_T20 512 592 (SEQ ID NO:64)

Segment cluster HSECADH_node_(—)12 (SEQ ID NO: 83) according to the present invention is supported by 26 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HSECADH_T11 (SEQ ID NO: 61), HSECADH_T18 (SEQ ID NO: 62), HSECADH_T19 (SEQ ID NO: 63) and HSECADH_T20 (SEQ ID NO: 64). Table 33 below describes the starting and ending position of this segment on each transcript.

TABLE 33 Segment location on transcripts Segment Segment Transcript starting ending name position position HSECADH_T11 593 655 (SEQ ID NO:61) HSECADH_T18 593 655 (SEQ ID NO:62) HSECADH_T19 593 655 (SEQ ID NO:63) HSECADH_T20 593 655 (SEQ ID NO:64)

Segment cluster HSECADH_node_(—)17 (SEQ ID NO: 84) according to the present invention can be found in the following transcript(s): HSECADH_T11 (SEQ ID NO: 61), HSECADH_T18 (SEQ ID NO: 62) and HSECADH_T19 (SEQ ID NO: 63). Table 34 below describes the starting and ending position of this segment on each transcript.

TABLE 34 Segment location on transcripts Segment Segment Transcript starting ending name position position HSECADH_T11 812 827 (SEQ ID NO:61) HSECADH_T18 812 827 (SEQ ID NO:62) HSECADH_T19 812 827 (SEQ ID NO:63)

Segment cluster HSECADH_node_(—)18 (SEQ ID NO: 85) according to the present invention is supported by 41 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HSECADH_T11 (SEQ ID NO: 61), HSECADH_T18 (SEQ ID NO: 62) and HSECADH_T19 (SEQ ID NO: 63). Table 35 below describes the starting and ending position of this segment on each transcript.

TABLE 35 Segment location on transcripts Segment Segment Transcript starting ending name position position HSECADH_T11 828 944 (SEQ ID NO:61) HSECADH_T18 828 944 (SEQ ID NO:62) HSECADH_T19 828 944 (SEQ ID NO:63)

Segment cluster HSECADH_node_(—)19 (SEQ ID NO: 86) according to the present invention can be found in the following transcript(s): HSECADH_T18 (SEQ ID NO: 62) and HSECADH_T19 (SEQ ID NO: 63). Table 36 below describes the starting and ending position of this segment on each transcript.

TABLE 36 Segment location on transcripts Segment Segment Transcript starting ending name position position HSECADH_T18 945 956 (SEQ ID NO:61) HSECADH_T19 945 956 (SEQ ID NO:63)

Segment cluster HSECADH_node_(—)3 (SEQ ID NO: 87) according to the present invention is supported by 18 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HSECADH_T11 (SEQ ID NO: 61), HSECADH_T18 (SEQ ID NO: 62), HSECADH_T19 (SEQ ID NO: 63) and HSECADH_T20 (SEQ ID NO: 64). Table 37 below describes the starting and ending position of this segment on each transcript.

TABLE 37 Segment location on transcripts Segment Segment Transcript starting ending name position position HSECADH_T11 173 287 (SEQ ID NO:61) HSECADH_T18 173 287 (SEQ ID NO:62) HSECADH_T19 173 287 (SEQ ID NO:63) HSECADH_T20 173 287 (SEQ ID NO:64)

Segment cluster HSECADH_node_(—)42 (SEQ ID NO: 88) according to the present invention is supported by 43 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HSECADH_T11 (SEQ ID NO: 61). Table 38 below describes the starting and ending position of this segment on each transcript.

TABLE 38 Segment location on transcripts Segment Segment Transcript name starting position ending position HSECADH_T11 945 1017 (SEQ ID NO: 61)

Segment cluster HSECADH_node_(—)45 (SEQ ID NO: 89) according to the present invention is supported by 39 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HSECADH_T11 (SEQ ID NO: 61). Table 39 below describes the starting and ending position of this segment on each transcript.

TABLE 39 Segment location on transcripts Segment Segment Transcript name starting position ending position HSECADH_T11 1018 1051 (SEQ ID NO: 61)

Segment cluster HSECADH_node_(—)46 (SEQ ID NO: 90) according to the present invention is supported by 40 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HSECADH_T11 (SEQ ID NO: 61). Table 40 below describes the starting and ending position of this segment on each transcript.

TABLE 40 Segment location on transcripts Segment Segment Transcript name starting position ending position HSECADH_T11 1052 1148 (SEQ ID NO: 61)

Segment cluster HSECADH_node_(—)55 (SEQ ID NO: 91) according to the present invention is supported by 36 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HSECADH_T11 (SEQ ID NO: 61). Table 41 below describes the starting and ending position of this segment on each transcript.

TABLE 41 Segment location on transcripts Segment Segment Transcript name starting position ending position HSECADH_T11 2054 2166 (SEQ ID NO: 61)

Segment cluster HSECADH_node_(—)56 (SEQ ID NO: 92) according to the present invention is supported by 42 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HSECADH_T11 (SEQ ID NO: 61). Table 42 below describes the starting and ending position of this segment on each transcript.

TABLE 42 Segment location on transcripts Segment Segment Transcript name starting position ending position HSECADH_T11 2167 2240 (SEQ ID NO: 61)

Segment cluster HSECADH_node_(—)58 (SEQ ID NO: 93) according to the present invention is supported by 61 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HSECADH_T11 (SEQ ID NO: 61). Table 43 below describes the starting and ending position of this segment on each transcript.

TABLE 43 Segment location on transcripts Segment Segment Transcript name starting position ending position HSECADH_T11 2431 2481 (SEQ ID NO: 61)

Segment cluster HSECADH_node_(—)59 (SEQ ID NO: 94) according to the present invention can be found in the following transcript(s): HSECADH_T11 (SEQ ID NO: 61). Table 44 below describes the starting and ending position of this segment on each transcript.

TABLE 44 Segment location on transcripts Segment Segment Transcript name starting position ending position HSECADH_T11 2482 2503 (SEQ ID NO: 61) Variant protein alignment to the previously known protein: Sequence name: /tmp/2x0I2XZlA3/JXvUszCm3O:Q9UII7 (SEQ ID NO: 963) Sequence documentation:

Alignment of: HSECADH_P9 (SEQ ID NO: 96) x Q9UII7 (SEQ ID NO: 963) ..

Alignment segment 1/1:

Quality: 2727.00 Escore: 0 Matching length: 274 Total length: 274 Matching Percent Similarity: 100.00 Matching Percent Identity: 100.00 Total Percent Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0

Alignment:

Sequence name: /tmp/2x0I2XZlA3/JXvUszCm3O:Q9UII8 (SEQ ID NO: 964) Sequence documentation:

Alignment of: HSECADH_P9 (SEQ ID NO: 96) x Q9UII8 (SEQ ID NO: 964) ..

Alignment segment 1/1:

Quality: 2727.00 Escore: 0 Matching length: 274 Total length: 274 Matching Percent Similarity: 100.00 Matching Percent Identity: 100.00 Total Percent Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0

Alignment:

Sequence name: /tmp/2x0I2XZlA3/JXvUszCm3O:CAD1_HUMAN Sequence documentation:

Alignment of: HSECADH_P9 (SEQ ID NO: 96) x CAD1_HUMAN ..

Alignment segment 1/1:

Quality: 2727.00 Escore: 0 Matching length: 274 Total length: 274 Matching Percent Similarity: 100.00 Matching Percent Identity: 100.00 Total Percent Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0

Alignment:

Sequence name: /tmp/e5Y8HiBmjB/iwybld81kl:Q9UII7 (SEQ ID NO: 963) Sequence documentation:

Alignment of: HSECADH_P13 (SEQ ID NO: 97) x Q9UII7 (SEQ ID NO: 963) ..

Alignment segment 1/1:

Quality: 3720.00 Escore: 0 Matching length: 379 Total length: 379 Matching Percent Similarity: 100.00 Matching Percent Identity: 100.00 Total Percent Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0

Alignment:

Sequence name: /tmp/e5Y8HiBmjB/iwybld81kl:Q9UII8 (SEQ ID NO: 964) Sequence documentation:

Alignment of: HSECADH_P13 (SEQ ID NO: 97) x Q9UII8 (SEQ ID NO: 964) ..

Alignment segment 1/1:

Quality: 3720.00 Escore: 0 Matching length: 379 Total length: 379 Matching Percent Similarity: 100.00 Matching Percent Identity: 100.00 Total Percent Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0

Alignment:

Sequence name: /tmp/e5Y8HiBmjB/iwybld81kl:CAD1_HUMAN Sequence documentation:

Alignment of: HSECADH_P13 (SEQ ID NO: 97) x CAD1_HUMAN ..

Alignment segment 1/1:

Quality: 3720.00 Escore: 0 Matching length: 379 Total length: 379 Matching Percent Similarity: 100.00 Matching Percent Identity: 100.00 Total Percent Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0

Alignment:

Sequence name: /tmp/RtiX8vFyZe/iovNeRHKWU:Q9UII7 (SEQ ID NO: 963) Sequence documentation:

Alignment of: HSECADH_P14 (SEQ ID NO: 98) x Q9UII7 (SEQ ID NO: 963) ..

Alignment segment 1/1:

Quality: 3313.00 Escore: 0 Matching length: 336 Total length: 336 Matching Percent Similarity: 100.00 Matching Percent Identity: 100.00 Total Percent Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0

Alignment:

Sequence name: /tmp/RtiX8vFyZe/iovNeRHKWU:Q9UII8 (SEQ ID NO: 964) Sequence documentation:

Alignment of: HSECADH_P14 (SEQ ID NO: 98) x Q9UII8 (SEQ ID NO: 964) ..

Alignment segment 1/1:

Quality: 3313.00 Escore: 0 Matching length: 336 Total length: 336 Matching Percent Similarity: 100.00 Matching Percent Identity: 100.00 Total Percent Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0

Alignment:

Sequence name: /tmp/RtiX8vFyZe/iovNeRHKWU:CAD1_HUMAN Sequence documentation:

Alignment of: HSECADH_P14 (SEQ ID NO: 98) x CAD1_HUMAN ..

Alignment segment 1/1:

Quality: 3313.00 Escore: 0 Matching length: 336 Total length: 336 Matching Percent Similarity: 100.00 Matching Percent Identity: 100.00 Total Percent Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0

Alignment:

Sequence name: /tmp/rMRrwmuokD/1rmk2jOfgw:Q9UII7 (SEQ ID NO: 963) Sequence documentation:

Alignment of: HSECADH_P15 (SEQ ID NO: 99) x Q9UII7 (SEQ ID NO: 963) ..

Alignment segment 1/1:

Quality: 2289.00 Escore: 0 Matching length: 229 Total length: 229 Matching Percent Similarity: 100.00 Matching Percent Identity: 100.00 Total Percent Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0

Alignment:

Sequence name: /tmp/rMRrwmuokD/1rmk2jOfgw:Q9UII8 (SEQ ID NO: 964) Sequence documentation:

Alignment of: HSECADH_P15 (SEQ ID NO: 99) x Q9UII8 (SEQ ID NO: 964) ..

Alignment segment 1/1:

Quality: 2289.00 Escore: 0 Matching length: 229 Total length: 229 Matching Percent Similarity: 100.00 Matching Percent Identity: 100.00 Total Percent Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0

Alignment:

Sequence name: /tmp/rMRrwmuokD/1rmk2jOfgw:CAD1_HUMAN Sequence documentation:

Alignment of: HSECADH_P15 (SEQ ID NO: 99) x CAD1_HUMAN ..

Alignment segment 1/1:

Quality: 2289.00 Escore: 0 Matching length: 229 Total length: 229 Matching Percent Similarity: 100.00 Matching Percent Identity: 100.00 Total Percent Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0

Alignment:

Description for Cluster HUMGRP5E

Cluster HUMGRP5E features 2 transcript(s) and 5 segment(s) of interest, the names for which are given in Tables 1 and 2, respectively, the sequences themselves are given at the end of the application. The selected protein variants are given in table 3.

TABLE 1 Transcripts of interest Transcript Name SEQ ID NO: HUMGRP5E_T4 100 (SEQ ID NO:100) HUMGRP5E_T5 101 (SEQ ID NO:101)

TABLE 2 Segments of interest Segment Name SEQ ID NO: HUMGRP5E_node_0 102 (SEQ ID NO:102) HUMGRP5E_node_2 103 (SEQ ID NO:103) HUMGRP5E_node_8 104 (SEQ ID NO:104) HUMGRP5E_node_3 105 (SEQ ID NO:105) HUMGRP5E_node_7 106 (SEQ ID NO:106)

TABLE 3 Proteins of interest Protein Name SEQ ID NO: HUMGRP5E_P4 108 (SEQ ID NO:108) HUMGRP5E_P5 109 (SEQ ID NO:109)

These sequences are variants of the known protein Gastrin-releasing peptide precursor (SwissProt accession identifier GRP_HUMAN; known also according to the synonyms GRP; GRP-10), SEQ ID NO: 107, referred to herein as the previously known protein.

Gastrin-releasing peptide is known or believed to have the following function(s): stimulates gastrin release as well as other gastrointestinal hormones. The sequence for protein Gastrin-releasing peptide precursor is given at the end of the application, as “Gastrin-releasing peptide precursor amino acid sequence”. Known polymorphisms for this sequence are as shown in Table 4.

TABLE 4 Amino acid mutations for Known Protein SNP position(s) on amino acid sequence Comment 4 S -> R

Protein Gastrin-releasing peptide localization is believed to be Secreted. The previously known protein also has the following indication(s) and/or potential therapeutic use(s): Diabetes, Type II. It has been investigated for clinical/therapeutic use in humans, for example as a target for an antibody or small molecule, and/or as a direct therapeutic; available information related to these investigations is as follows. Potential pharmaceutically related or therapeutically related activity or activities of the previously known protein are as follows: Bombesin antagonist; Insulinotropin agonist. A therapeutic role for a protein represented by the cluster has been predicted. The cluster was assigned this field because there was information in the drug database or the public databases (e.g., described herein above) that this protein, or part thereof, is used or can be used for a potential therapeutic indication: Anorectic/Antiobesity; Releasing hormone; Anticancer; Respiratory; Antidiabetic.

The following GO Annotation(s) apply to the previously known protein. The following annotation(s) were found: signal transduction; neuropeptide signaling pathway, which are annotation(s) related to Biological Process; growth factor, which are annotation(s) related to Molecular Function; and secreted, which are annotation(s) related to Cellular Component.

The GO assignment relies on information from one or more of the SwissProt/TremBl Protein knowledgebase, available from <http://www.expasy.ch/sprot/>; or Locuslink, available from <http://www.ncbi.nlm.nih.gov/projects/LocusLink/>.

As noted above, cluster HUMGRP5E features 2 transcript(s), which were listed in Table 1 above. These transcript(s) encode for protein(s) which are variant(s) of protein Gastrin-releasing peptide precursor. A description of each variant protein according to the present invention is now provided.

Variant protein HUMGRP5E_P4 (SEQ ID NO: 108) according to the present invention has an amino acid sequence as given at the end of the application; it is encoded by transcript(s) HUMGRP5E_T4 (SEQ ID NO: 100). An alignment is given to the known protein (Gastrin-releasing peptide precursor) at the end of the application. One or more alignments to one or more previously published protein sequences are given at the end of the application. A brief description of the relationship of the variant protein according to the present invention to each such aligned protein is as follows:

Comparison report between HUMGRP5E_P4 (SEQ ID NO: 108) and GRP_HUMAN:

1. An isolated chimeric polypeptide encoding for HUMGRP5E_P4 (SEQ ID NO: 108), comprising a first amino acid sequence being at least 90% homologous to MRGSELPLVLLALVLCLAPRGRAVPLPAGGGTVLTKMYPRGNHWAVGHLMGKKSTG ESSSVSERGSLKQQLREYIRWEEAARNLLGLIEAKENRNHQPPQPKALGNQQPSWDSED SSNFKDVGSKGK corresponding to amino acids 1-127 of GRP_HUMAN, which also corresponds to amino acids 1-127 of HUMGRP5E_P4 (SEQ ID NO: 108), and a second amino acid sequence being at least 90% homologous to GSQREGRNPQLNQQ corresponding to amino acids 135-148 of GRP_HUMAN, which also corresponds to amino acids 128-141 of HUMGRP5E_P4 (SEQ ID NO: 108), wherein said first and second amino acid sequences are contiguous and in a sequential order.

2. An isolated chimeric polypeptide encoding for an edge portion of HUMGRP5E_P4 (SEQ ID NO: 108), comprising a polypeptide having a length “n”, wherein n is at least about 10 amino acids in length, optionally at least about 20 amino acids in length, preferably at least about 30 amino acids in length, more preferably at least about 40 amino acids in length and most preferably at least about 50 amino acids in length, wherein at least two amino acids comprise KG, having a structure as follows: a sequence starting from any of amino acid numbers 127-x to 127; and ending at any of amino acid numbers 128+((n−2)−x), in which x varies from 0 to n−2.

The location of the variant protein was determined according to results from a number of different software programs and analyses, including analyses from SignalP and other specialized programs. The variant protein is believed to be located as follows with regard to the cell: secreted. The protein localization is believed to be secreted because both signal-peptide prediction programs predict that this protein has a signal peptide, and neither trans-membrane region prediction program predicts that this protein has a trans-membrane region.

Variant protein HUMGRP5E_P4 (SEQ ID NO: 108) also has the following non-silent SNPs (Single Nucleotide Polymorphisms) as listed in Table 5, (given according to their position(s) on the amino acid sequence, with the alternative amino acid(s) listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein HUMGRP5E_P4 (SEQ ID NO: 108) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 5 Amino acid mutations SNP position(s) on amino acid Alternative amino Previously known sequence acid(s) SNP? 4 S -> R Yes

Variant protein HUMGRP5E_P4 (SEQ ID NO: 108) is encoded by the following transcript(s): HUMGRP5E_T4 (SEQ ID NO: 100), for which the sequence(s) is/are given at the end of the application. The coding portion of transcript HUMGRP5E_T4 (SEQ ID NO: 100) is shown in bold; this coding portion starts at position 622 and ends at position 1044. The transcript also has the following SNPs as listed in Table 6 (given according to their position on the nucleotide sequence, with the alternative nucleic acid listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein HUMGRP5E_P4 (SEQ ID NO: 108) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 6 Nucleic acid SNPs SNP position on nucleotide Alternative sequence nucleic acid Previously known SNP? 541 -> T No 542 G -> T No 631 A -> C Yes 672 G -> A Yes 1340 C -> No 1340 C -> A No 1341 A -> No 1341 A -> G No

Variant protein HUMGRP5E_P5 (SEQ ID NO: 109) according to the present invention has an amino acid sequence as given at the end of the application; it is encoded by transcript(s) HUMGRP5E_T5 (SEQ ID NO: 101). An alignment is given to the known protein (Gastrin-releasing peptide precursor) at the end of the application. One or more alignments to one or more previously published protein sequences are given at the end of the application. A brief description of the relationship of the variant protein according to the present invention to each such aligned protein is as follows:

Comparison report between HUMGRP5E_P5 (SEQ ID NO: 109) and GRP_HUMAN:

1. An isolated chimeric polypeptide encoding for HUMGRP5E_P5 (SEQ ID NO: 109), comprising a first amino acid sequence being at least 90% homologous to MRGSELPLVLLALVLCLAPRGRAVPLPAGGGTVLTKMYPRGNHWAVGHLMGKKSTG

-   -   20 ESSSVSERGSLKQQLREYIRWEEAARNLLGLIEAKENRNHQPPQPKALGNQQPSWDSED         SSNFKDVGSKGK corresponding to amino acids 1-127 of GRP_HUMAN,         which also corresponds to amino acids 1-127 of HUMGRP5E_P5 (SEQ         ID NO: 109), and a second amino acid sequence being at least         70%, optionally at least 80%, preferably at least 85%, more         preferably at least 90% and most preferably at least 95%         homologous to a polypeptide having the sequence DSLLQVLNVKEGTPS         (SEQ ID NO: 1125) corresponding to amino acids 128-142 of         HUMGRP5E_P5 (SEQ ID NO: 109), wherein said first and second         amino acid sequences are contiguous and in a sequential order.

2. An isolated polypeptide encoding for a tail of HUMGRP5E_P5 (SEQ ID NO: 109), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence DSLLQVLNVKEGTPS (SEQ ID NO: 1125) in HUMGRP5E_P5 (SEQ ID NO: 109).

The location of the variant protein was determined according to results from a number of different software programs and analyses, including analyses from SignalP and other specialized programs. The variant protein is believed to be located as follows with regard to the cell: secreted. The protein localization is believed to be secreted because both signal-peptide prediction programs predict that this protein has a signal peptide, and neither trans-membrane region prediction program predicts that this protein has a trans-membrane region.

Variant protein HUMGRP5E_P5 (SEQ ID NO: 109) also has the following non-silent SNPs (Single Nucleotide Polymorphisms) as listed in Table 7, (given according to their position(s) on the amino acid sequence, with the alternative amino acid(s) listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein HUMGRP5E_P5 (SEQ ID NO: 109) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 7 Amino acid mutations SNP position(s) on amino acid Alternative Previously known sequence amino acid(s) SNP? 4 S -> R Yes

Variant protein HUMGRP5E P5 (SEQ ID NO: 109) is encoded by the following transcript(s): HUMGRP5E_T5 (SEQ ID NO: 101), for which the sequence(s) is/are given at the end of the application. The coding portion of transcript HUMGRP5E_T5 (SEQ ID NO: 101) is shown in bold; this coding portion starts at position 622 and ends at position 1047. The transcript also has the following SNPs as listed in Table 8 (given according to their position on the nucleotide sequence, with the alternative nucleic acid listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein HUMGRP5E_P5 (SEQ ID NO: 109) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 8 Nucleic acid SNPs SNP position on nucleotide Previously known sequence Alternative nucleic acid SNP? 541 -> T No 542 G -> T No 631 A -> C Yes 672 G -> A Yes 1354 C -> No 1354 C -> A No 1355 A -> No 1355 A -> G No

As noted above, cluster HUMGRP5E features 5 segment(s), which were listed in Table 2 above and for which the sequence(s) are given at the end of the application. These segment(s) are portions of nucleic acid sequence(s) which are described herein separately because they are of particular interest. A description of each segment according to the present invention is now provided.

Segment cluster HUMGRP5E_node_(—)0 (SEQ ID NO: 102) according to the present invention is supported by 21 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HUMGRP5E_T4 (SEQ ID NO: 100) and HUMGRP5E_T5 (SEQ ID NO: 101). Table 9 below describes the starting and ending position of this segment on each transcript.

TABLE 9 Segment location on transcripts Segment Segment Transcript starting ending Name position position HUMGRP5E_T4 1 760 (SEQ ID NO:100) HUMGRP5E_T5 1 760 (SEQ ID NO:101)

Segment cluster HUMGRP5E_node_(—)2 (SEQ ID NO: 103) according to the present invention is supported by 27 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HUMGRP5E_T4 (SEQ ID NO: 100) and HUMGRP5E_T5 (SEQ ID NO: 101). Table 10 below describes the starting and ending position of this segment on each transcript.

TABLE 10 Segment location on transcripts Segment Segment Transcript starting ending Name position position HUMGRP5E_T4 761 984 (SEQ ID NO:100) HUMGRP5E_T5 761 984 (SEQ ID NO:101)

Segment cluster HUMGRP5E_node_(—)8 (SEQ ID NO: 104) according to the present invention is supported by 26 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HUMGRP5E_T4 (SEQ ID NO: 100) and HUMGRP5E_T5 (SEQ ID NO: 101). Table 11 below describes the starting and ending position of this segment on each transcript.

Table 11—Segment Location on Transcripts

TABLE 11 Segment location on transcripts Segment Segment Transcript starting ending Name position position HUMGRP5E_T4 1004 1362 (SEQ ID NO:100) HUMGRP5E_T5 1018 1376 (SEQ ID NO:101)

According to an optional embodiment of the present invention, short segments related to the above cluster are also provided. These segments are up to about 120 bp in length, and so are included in a separate description.

Segment cluster HUMGRP5E_node_(—)3 (SEQ ID NO: 105) according to the present invention can be found in the following transcript(s): HUMGRP5E_T4 (SEQ ID NO: 100) and HUMGRP5E T5 (SEQ ID NO: 101). Table 12 below describes the starting and ending position of this segment on each transcript.

TABLE 12 Segment location on transcripts Segment Segment Transcript starting ending Name position position HUMGRP5E_T4 985 1003 (SEQ ID NO:100) HUMGRP5E_T5 985 1003 (SEQ ID NO:101)

Segment cluster HUMGRP5E_node_(—)7 (SEQ ID NO: 106) according to the present invention can be found in the following transcript(s): HUMGRP5E_T5 (SEQ ID NO: 101). Table 13 below describes the starting and ending position of this segment on each transcript.

TABLE 13 Segment location on transcripts Segment Segment Transcript starting ending Name position position HUMGRP5E_T5 1004 1017 (SEQ ID NO:101) Variant protein alignment to the previously known protein: Sequence name: /tmp/412zs2mwyT/B0wjOUAX0d:GRP_HUMAN Sequence documentation:

Alignment of: HUMGRP5E_P4 (SEQ ID NO: 108) x GRP_HUMAN ..

Alignment segment 1/1:

Quality: 1291.00 Escore: 0 Matching length: 141 Total length: 148 Matching Percent Similarity: 100.00 Matching Percent Identity: 100.00 Total Percent Similarity: 95.27 Total Percent Identity: 95.27 Gaps: 1

Alignment:

Sequence name: /tmp/1me9ldnvfv/KbP5io8PtU:GRP_HUMAN Sequence documentation:

Alignment of: HUMGRP5E_P5 (SEQ ID NO: 109) x GRP_HUMAN ..

Alignment segment 1/1:

Quality: 1248.00 Escore: 0 Matching length: 127 Total length: 127 Matching Percent Similarity: 100.00 Matching Percent Identity: 100.00 Total Percent Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0

Alignment:

Expression of GRP_HUMAN—gastrin-releasing peptide HUMGRP5E transcripts which are detectable by amplicon as depicted in sequence name HUMGRP5E junc3-7 (SEQ ID NO:967) in normal and cancerous ovary tissues

Expression of GRP_HUMAN—gastrin-releasing peptide transcripts detectable by or according to junc3-7, HUMGRP5Ejunc3-7 amplicon(s) (SEQ ID NO:967) and HUMGRP5Ejunc3-7F (SEQ ID NO:965) and HUMGRP5Ejunc3-7R (SEQ ID NO:966) primers was measured by real time PCR. In parallel the expression of four housekeeping genes PBGD (GenBank Accession No. BC019323, (SEQ ID NO: 1036); amplicon—PBGD-amplicon, (SEQ ID NO: 1039)), HPRT1 (GenBank Accession No. NM_(—)000194, (SEQ ID NO:1040); amplicon—HPRT1-amplicon, (SEQ ID NO: 1043) and SDHA (GenBank Accession No. NM_(—)004168, (SEQ ID NO: 1032); amplicon—SDHA-amplicon, (SEQ ID NO: 1035)), GAPDH (GenBank Accession No. BC026907, (SEQ ID NO: 1044); GAPDH amplicon, (SEQ ID NO: 1047)) was measured similarly. For each RT sample, the expression of the above amplicon was normalized to the geometric mean of the quantities of the housekeeping genes. The normalized quantity of each RT sample was then divided by the median of the quantities of the normal post-mortem (PM) samples (Sample No 45-48, 71 Table 1 above, “Tissue samples in testing panel”), to obtain a value of fold up-regulation for each sample relative to median of the normal PM samples.

FIG. 13 is a histogram showing over expression of the above-indicated GRP_HUMAN—gastrin-releasing peptide transcripts in cancerous ovary samples relative to the normal samples. Values represent the average of duplicate experiments. Error bars indicate the minimal and maximal values obtained). As is evident from FIG. 13, the expression of GRP_HUMAN—gastrin-releasing peptide transcripts detectable by the above amplicon(s) in several cancer samples was higher in several cancerous samples than in the non-cancerous samples (Sample No. 45, 47-48, 71 Table 1 above, “Tissue samples in testing panel”) and including benign samples (samples No. 57-62 Table 1 above, “Tissue samples in testing panel”). Notably an over-expression of at least 5 fold was found in 13 out of 43 adenocarcinoma samples.

Primer pairs are also optionally and preferably encompassed within the present invention; for example, for the above experiment, the following primer pair was used as a non-limiting illustrative example only of a suitable primer pair: HUMGRP5Ejunc3-7F (SEQ ID NO:965) forward primer; and HUMGRP5Ejunc3-7R (SEQ ID NO:966) reverse primer.

The present invention also preferably encompasses any amplicon obtained through the use of any suitable primer pair; for example, for the above experiment, the following amplicon was obtained as a non-limiting illustrative example only of a suitable amplicon: HUMGRP5Ejunc3-7 (SEQ ID NO:967).

HUMGRP5Ejunc3-7F (SEQ ID NO:965) ACCAGCCACCTCAACCCA HUMGRP5Ejunc3-7R (SEQ ID NO:966) CTGGAGCAGAGAGTCTTTGCCT HUMGRP5Ejunc3-7 (SEQ ID NO:967) ACCAGCCACCTCAACCCAAGGCCCTGGGCAATCAGCAGCCTTCGTGGGAT TCAGAGGATAGCAGCAACTTCAAAGATGTAGGTTCAAAAGGCAAAGACTC TCTGCTCCAG

Expression of GRP_HUMAN—gastrin-releasing peptide HUMGRP5E transcripts, which are detectable by amplicon as depicted in sequence name HUMGRP5E junc3-7 (SEQ ID NO:967) in different normal tissues.

Expression of GRP_HUMAN—gastrin-releasing peptide transcripts detectable by or according to HUMGRP5E junc3-7 amplicon(s) (SEQ ID NO:967) and HUMGRP5E junc3-7F (SEQ ID NO:965) and HUMGRP5Ejunc3-7R (SEQ ID NO:966) was measured by real time PCR. In parallel the expression of four housekeeping genes -RPL19 (GenBank Accession No. NM_(—)000981, (SEQ ID NO:1048); RPL19 amplicon, (SEQ ID NO:1051)), TATA box (GenBank Accession No. NM_(—)003194, (SEQ ID NO:1052); TATA amplicon, (SEQ ID NO:1055)), Ubiquitin (GenBank Accession No. BC000449, (SEQ ID NO: 1056); amplicon—Ubiquitin-amplicon, (SEQ ID NO: 1059)) and SDHA (GenBank Accession No. NM_(—)004168, (SEQ ID NO: 1032); amplicon—SDHA-amplicon, (SEQ ID NO: 1035)) was measured similarly. For each RT sample, the expression of the above amplicon was normalized to the geometric mean of the quantities of the housekeeping genes. The normalized quantity of each RT sample was then divided by the median of the quantities of the breast samples (Sample Nos. 33-35 above), to obtain a value of relative expression of each sample relative to median of the breast samples.

The results are described in FIG. 14, presenting the histogram showing the expression of HUMGRP5E transcripts, which are detectable by amplicon as depicted in sequence name HUMGRP5E junc3-7 (SEQ ID NO:967), in different normal tissues. Primers and amplicons are as above.

Description for Cluster R11723

Cluster R11723 features 6 transcript(s) and 26 segment(s) of interest, the names for which are given in Tables 1 and 2, respectively, the sequences themselves are given at the end of the application. The selected protein variants are given in table 3.

TABLE 1 Transcripts of interest Transcript Name SEQ ID NO: R11723_PEA_1_T15 110 R11723_PEA_1_T17 111 R11723_PEA_1_T19 112 R11723_PEA_1_T20 113 R11723_PEA_1_T5 114 R11723_PEA_1_T6 115

TABLE 2 Segments of interest Segment Name SEQ ID NO: R11723_PEA_1_node_13 116 R11723_PEA_1_node_16 117 R11723_PEA_1_node_19 118 R11723_PEA_1_node_2 119 R11723_PEA_1_node_22 120 R11723_PEA_1_node_31 121 R11723_PEA_1_node_10 122 R11723_PEA_1_node_11 123 R11723_PEA 1_node_15 124 R11723_PEA 1_node_18 125 R11723_PEA_1_node_20 126 R11723_PEA_1_node_21 127 R11723_PEA_1_node_23 128 R11723_PEA_1_node_24 129 R11723_PEA_1_node_25 130 R11723_PEA_1_node_26 131 R11723_PEA_1_node_27 132 R11723_PEA_1_node_28 133 R11723_PEA_1_node_29 134 R11723_PEA_1_node_3 135 R11723_PEA_1_node_30 136 R11723_PEA_1_node_4 137 R11723_PEA_1_node_5 138 R11723_PEA_1_node_6 139 R11723_PEA_1_node_7 140 R11723_PEA_1_node_8 141

TABLE 3 Proteins of interest Protein Name SEQ ID NO: R11723_PEA_1_P2 142 R11723_PEA_1_P6 143 R11723_PEA_1_P7 144 R11723_PEA_1_P13 145 R11723_PEA_1_P10 146

Cluster R11723 can be used as a diagnostic marker according to overexpression of transcripts of this cluster in cancer. Expression of such transcripts in normal tissues is also given according to the previously described methods. The term “number” in the right hand column of the table and the numbers on the y-axis of FIG. 15 refer to weighted expression of ESTs in each category, as “parts per million” (ratio of the expression of ESTs for a particular cluster to the expression of all ESTs in that category, according to parts per million).

Overall, the following results were obtained as shown with regard to the histograms in FIG. 15 and Table 4. This cluster is overexpressed (at least at a minimum level) in the following pathological conditions: epithelial malignant tumors, a mixture of malignant tumors from different tissues and kidney malignant tumors.

TABLE 4 Normal tissue distribution Name of Tissue Number adrenal 0 brain 30 epithelial 3 general 17 head and neck 0 kidney 0 lung 0 breast 0 ovary 0 pancreas 10 skin 0 uterus 0

TABLE 5 P values and ratios for expression in cancerous tissue Name of Tissue P1 P2 SP1 R3 SP2 R4 adrenal 4.2e−01 4.6e−01 4.6e−01 2.2 5.3e−01 1.9 brain 2.2e−01 2.0e−01 1.2e−02 2.8 5.0e−02 2.0 epithelial 3.0e−05 6.3e−05 1.8e−05 6.3 3.4e−06 6.4 general 7.2e−03 4.0e−02 1.3e−04 2.1 1.1e−03 1.7 head and neck 1 5.0e−01 1 1.0 7.5e−01 1.3 kidney 1.5e−01 2.4e−01 4.4e−03 5.4 2.8e−02 3.6 lung 1.2e−01 1.6e−01 1 1.6 1 1.3 breast 5.9e−01 4.4e−01 1 1.1 6.8e−01 1.5 ovary 1.6e−02 1.3e−02 1.0e−01 3.8 7.0e−02 3.5 pancreas 5.5e−01 2.0e−01 3.9e−01 1.9 1.4e−01 2.7 skin 1 4.4e−01 1 1.0 1.9e−02 2.1 uterus 1.5e−02 5.4e−02 1.9e−01 3.1 1.4e−01 2.5

It should be noted that the variants of this cluster are variants of the hypothetical protein PSEC0181 (referred to herein as “PSEC”). Furthermore, use of the known protein (WT protein) for detection of ovarian cancer, alone or in combination with one or more variants of this cluster and/or of any other cluster and/or of any known marker, also comprises an embodiment of the present invention. As described in greater detail below, in ovarian cancer, the variants of the present invention show a similar expression patter to that of PSEC, except that at least one variant shows greater overexpression than PSEC in ovarian cancer.

As noted above, cluster R11723 features 6 transcript(s), which were listed in Table 1 above. A description of each variant protein according to the present invention is now provided.

Variant protein R11723_PEA_(—)1_P2 (SEQ ID NO: 142) according to the present invention has an amino acid sequence as given at the end of the application; it is encoded by transcript(s) R11723_PEA_(—)1_T6 (SEQ ID NO: 115). The location of the variant protein was determined according to results from a number of different software programs and analyses, including analyses from SignalP and other specialized programs. The variant protein is believed to be located as follows with regard to the cell: secreted. The protein localization is believed to be secreted because both signal-peptide prediction programs predict that this protein has a signal peptide, and neither trans-membrane region prediction program predicts that this protein has a trans-membrane region.

Variant protein R11723_PEA_(—)1_P2 (SEQ ID NO: 142) also has the following non-silent SNPs (Single Nucleotide Polymorphisms) as listed in Table 6, (given according to their position(s) on the amino acid sequence, with the alternative amino acid(s) listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein R11723_PEA_(—)1_P2 (SEQ ID NO: 142) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 6 Amino acid mutations SNP position(s) on amino acid Alternative Previously known sequence amino acid(s) SNP? 107 H -> P Yes 70 G -> No 70 G -> C No

Variant protein R11723_PEA_(—)1_P2 (SEQ ID NO: 142) is encoded by the following transcript(s): R11723_PEA_(—)1_T6 (SEQ ID NO: 115), for which the sequence(s) is/are given at the end of the application. The coding portion of transcript R11723_PEA_(—)1_T6 (SEQ ID NO: 115) is shown in bold; this coding portion starts at position 1716 and ends at position 2051. The transcript also has the following SNPs as listed in Table 7 (given according to their position on the nucleotide sequence, with the alternative nucleic acid listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein R11723_PEA_(—)1_P2 (SEQ ID NO: 142) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 7 Nucleic acid SNPs SNP position on nucleotide Previously known sequence Alternative nucleic acid SNP? 1231 C -> T Yes 1278 G -> C Yes 1923 G -> No 1923 G -> T No 2035 A -> C Yes 2048 A -> C No 2057 A -> G Yes

Variant protein R11723_PEA_(—)1_P6 (SEQ ID NO: 143) according to the present invention has an amino acid sequence as given at the end of the application; it is encoded by transcript(s) R11723_PEA_(—)1_T15 (SEQ ID NO: 110). One or more alignments to one or more previously published protein sequences are given at the end of the application. A brief description of the relationship of the variant protein according to the present invention to each such aligned protein is as follows:

Comparison report between R11723_PEA_(—)1_P6 (SEQ ID NO: 143) and Q8IXM0 (SEQ ID NO: 968) (SEQ ID NO:968):

1. An isolated chimeric polypeptide encoding for R11723_PEA_(—)1_P6 (SEQ ID NO: 143), comprising a first amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence MWVLGIAATFCGLFLLPGFALQIQCYQCEEFQLNNDCSSPEFIVNCTVNVQDMCQKEV MEQSAGIMYRKSCASSAACLIASAGSPCRGLAPGREEQRALHKAGAVGGGVR (SEQ ID NO: 1126) corresponding to amino acids 1-110 of R11723_PEA_(—)1_P6 (SEQ ID NO: 143), and a second amino acid sequence being at least 90% homologous to MYAQALLVVGVLQRQAAAQHLHEHPPKLLRGHRVQERVDDRAEVEKRLREGEEDHV RPEVGPRPVVLGFGRSHDPPNLVGHPAYGQCHNNQPWADTSRRERQRKEKHSMRTQ corresponding to amino acids 1-112 of Q8IXM0 (SEQ ID NO: 968), which also corresponds to amino acids 111-222 of R11723_PEA_(—)1_P6 (SEQ ID NO: 143), wherein said first and second amino acid sequences are contiguous and in a sequential order.

2. An isolated polypeptide encoding for a head of R11723_PEA_(—)1_P6 (SEQ ID NO: 143), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence MWVLGIAATFCGLFLLPGFALQIQCYQCEEFQLNNDCSSPEFIVNCTVNVQDMCQKEV MEQSAGIMYRKSCASSAACLIASAGSPCRGLAPGREEQRALHKAGAVGGGVR (SEQ ID NO: 1126) of R11723_PEA_(—)1_P6 (SEQ ID NO: 143).

Comparison report between R11723_PEA_(—)1_P6 (SEQ ID NO: 143) and Q96AC2 (SEQ ID NO: 969) (SEQ ID NO:969):

1. An isolated chimeric polypeptide encoding for R11723_PEA_(—)1_P6 (SEQ ID NO: 143), comprising a first amino acid sequence being at least 90% homologous to MWVLGIAATFCGLFLLPGFALQIQCYQCEEFQLNNDCSSPEFIVNCTVNVQDMCQKEV MEQSAGIMYRKSCASSAACLIASAG corresponding to amino acids 1-83 of Q96AC2 (SEQ ID NO: 969), which also corresponds to amino acids 1-83 of R11723_PEA_(—)1_P6 (SEQ ID NO: 143), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence SPCRGLAPGREEQRALHKAGAVGGGVRMYAQALLVVGVLQRQAAAQHLHEHPPKLL RGHRVQERVDDRAEVEKRLREGEEDHVRPEVGPRPVVLGFGRSHDPPNLVGHPAYGQ CHNNQPWADTSRRERQRKEKHSMRTQ (SEQ ID NO: 1127) corresponding to amino acids 84-222 of R11723_PEA_(—)1_P6 (SEQ ID NO: 143), wherein said first and second amino acid sequences are contiguous and in a sequential order.

2. An isolated polypeptide encoding for a tail of R11723_PEA_(—)1_P6 (SEQ ID NO: 143), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence SPCRGLAPGREEQRALHKAGAVGGGVRMYAQALLVVGVLQRQAAAQHLHEHPPKLL RGHRVQERVDDRAEVEKRLREGEEDHVRPEVGPRPVVLGFGRSHDPPNLVGHPAYGQ CHNNQPWADTSRRERQRKEKHSMRTQ (SEQ ID NO: 1127) in R11723_PEA_(—)1_P6 (SEQ ID NO: 143).

Comparison report between R11723_PEA_(—)1_P6 (SEQ ID NO: 143) and Q8N2G4 (SEQ ID NO: 970) (SEQ ID NO:970):

1. An isolated chimeric polypeptide encoding for R11723_PEA_(—)1_P6 (SEQ ID NO: 143), comprising a first amino acid sequence being at least 90% homologous to MWVLGIAATFCGLFLLPGFALQIQCYQCEEFQLNNDCSSPEFIVNCTVNVQDMCQKEV MEQSAGIMYRKSCASSAACLIASAG corresponding to amino acids 1-83 of Q8N2G4 (SEQ ID NO: 970), which also corresponds to amino acids 1-83 of R11723_PEA_(—)1_P6 (SEQ ID NO: 143), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence SPCRGLAPGREEQRALHKAGAVGGGVRMYAQALLVVGVLQRQAAAQHLHEHPPKLL RGHRVQERVDDRAEVEKRLREGEEDHVRPEVGPRPVVLGFGRSHDPPNLVGHPAYGQ CHNNQPWADTSRRERQRKEKHSMRTQ (SEQ ID NO: 1127) corresponding to amino acids 84-222 of R11723_PEA_(—)1_P6 (SEQ ID NO: 143), wherein said first and second amino acid sequences are contiguous and in a sequential order.

2. An isolated polypeptide encoding for a tail of R11723_PEA_(—)1_P6 (SEQ ID NO: 143), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence SPCRGLAPGREEQRALHKAGAVGGGVRMYAQALLVVGVLQRQAAAQHLHEHPPKLL RGHRVQERVDDRAEVEKRLREGEEDHVRPEVGPRPVVLGFGRSHDPPNLVGHPAYGQ CHNNQPWADTSRRERQRKEKHSMRTQ (SEQ, ID NO:1127) in R11723_PEA_(—)1_P6 (SEQ ID NO: 143).

Comparison report between R11723_PEA_(—)1_P6 (SEQ ID NO: 143) and BAC85518 (SEQ ID NO: 971) (SEQ ID NO:971):

1. An isolated chimeric polypeptide encoding for R11723_PEA_(—)1_P6 (SEQ ID NO: 143), comprising a first amino acid sequence being at least 90% homologous to MWVLGIAATFCGLFLLPGFALQIQCYQCEEFQLNNDCSSPEFIVNCTVNVQDMCQKEV MEQSAGIMYRKSCASSAACLIASAG corresponding to amino acids 24-106 of BAC85518 (SEQ ID NO: 971), which also corresponds to amino acids 1-83 of R11723_PEA_(—)1_P6 (SEQ ID NO: 143), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence SPCRGLAPGREEQRALHKAGAVGGGVRMYAQALLVVGVLQRQAAAQHLHEHPPKLL RGHRVQERVDDRAEVEKRLREGEEDHVRPEVGPRPVVLGFGRSHDPPNLVGHPAYGQ CHNNQPWADTSRRERQRKEKHSMRTQ (SEQ ID NO: 1127) corresponding to amino acids 84-222 of R11723_PEA_(—)1_P6 (SEQ ID NO: 143), wherein said first and second amino acid sequences are contiguous and in a sequential order.

2. An isolated polypeptide encoding for a tail of R11723_PEA_(—)1_P6 (SEQ ID NO: 143), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence SPCRGLAPGREEQRALHKAGAVGGGVRMYAQALLVVGVLQRQAAAQHLHEHPPKLL RGHRVQERVDDRAEVEKRLREGEEDHVRPEVGPRPVVLGFGRSHDPPNLVGHPAYGQ CHNNQPWADTSRRERQRKEKHSMRTQ (SEQ ID NO: 1127) in R11723_PEA_(—)1_P6 (SEQ ID NO: 143).

The location of the variant protein was determined according to results from a number of different software programs and analyses, including analyses from SignalP and other specialized programs. The variant protein is believed to be located as follows with regard to the cell: secreted. The protein localization is believed to be secreted because both signal-peptide prediction programs predict that this protein has a signal peptide, and neither trans-membrane region prediction program predicts that this protein has a trans-membrane region.

Variant protein R11723_PEA_(—)1_P6 (SEQ ID NO: 143) also has the following non-silent SNPs (Single Nucleotide Polymorphisms) as listed in Table 8, (given according to their position(s) on the amino acid sequence, with the alternative amino acid(s) listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein R11723_PEA_(—)1_P6 (SEQ ID NO: 143) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 8 Amino acid mutations SNP position(s) on amino acid Alternative Previously known sequence amino acid(s) SNP? 180 G -> No 180 G -> C No 217 H -> P Yes

Variant protein R11723_PEA_(—)1_P6 (SEQ ID NO: 143) is encoded by the following transcript(s): R11723_PEA_(—)1_T15 (SEQ ID NO: 110), for which the sequence(s) is/are given at the end of the application. The coding portion of transcript R11723_PEA_(—)1_T15 (SEQ ID NO: 110) is shown in bold; this coding portion starts at position 434 and ends at position 1099. The transcript also has the following SNPs as listed in Table 9 (given according to their position on the nucleotide sequence, with the alternative nucleic acid listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein R11723_PEA_(—)1_P6 (SEQ ID NO: 143) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 9 Nucleic acid SNPs SNP position on nucleotide Previously known sequence Alternative nucleic acid SNP? 971 G -> No 971 G -> T No 1083 A -> C Yes 1096 A -> C No 1105 A -> G Yes

Variant protein R11723_PEA_(—)1_P7 (SEQ ID NO: 144) according to the present invention has an amino acid sequence as given at the end of the application; it is encoded by transcript(s) R11723_PEA_(—)1_T17 (SEQ ID NO: 111). One or more alignments to one or more previously published protein sequences are given at the end of the application. A brief description of the relationship of the variant protein according to the present invention to each such aligned protein is as follows:

Comparison report between R11723_PEA_(—)1_P7 (SEQ ID NO: 144) and Q96AC2 (SEQ ID NO: 969):

1. An isolated chimeric polypeptide encoding for R11723_PEA_(—)1_P7 (SEQ ID NO: 144), comprising a first amino acid sequence being at least 90% homologous to MWVLGIAATFCGLFLLPGFALQIQCYQCEEFQLNNDCSSPEFIVNCTVNVQDMCQKEV MEQSAG corresponding to amino acids 1-64 of Q96AC2 (SEQ ID NO: 969), which also corresponds to amino acids 1-64 of R11723_PEA_(—)1_P7 (SEQ ID NO: 144), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence SHCVTRLECSGTISAHCNLCLPGSNDHPT (SEQ ID NO: 1128) corresponding to amino acids 65-93 of R11723_PEA_(—)1_P7 (SEQ ID NO: 144), wherein said first and second amino acid sequences are contiguous and in a sequential order.

2. An isolated polypeptide encoding for a tail of R11723_PEA_(—)1_P7 (SEQ ID NO: 144), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence SHCVTRLECSGTISAHCNLCLPGSNDHPT (SEQ ID NO: 1128) in R11723_PEA_(—)1_P7 (SEQ ID NO: 144).

Comparison report between R11723_PEA_(—)1_P7 (SEQ ID NO: 144) and Q8N2G4 (SEQ ID NO: 970):

1. An isolated chimeric polypeptide encoding for R11723_PEA_(—)1_P7 (SEQ ID NO: 144), comprising a first amino acid sequence being at least 90% homologous to MWVLGIAATFCGLFLLPGFALQIQCYQCEEFQLNNDCSSPEFIVNCTVNVQDMCQKEV MEQSAG corresponding to amino acids 1-64 of Q8N2G4 (SEQ ID NO: 970), which also corresponds to amino acids 1-64 of R11723_PEA_(—)1_P7 (SEQ ID NO: 144), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence SHCVTRLECSGTISAHCNLCLPGSNDHPT (SEQ ID NO: 1128) corresponding to amino acids 65-93 of R11723_PEA_(—)1_P7 (SEQ ID NO: 144), wherein said first and second amino acid sequences are contiguous and in a sequential order.

2. An isolated polypeptide encoding for a tail of R11723_PEA_(—)1_P7 (SEQ ID NO: 144), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence SHCVTRLECSGTISAHCNLCLPGSNDHPT (SEQ ID NO: 1128) in R11723_PEA_(—)1_P7 (SEQ ID NO: 144).

Comparison report between R11723_PEA_(—)1_P7 (SEQ ID NO: 144) and BAC85273 (SEQ ID NO: 972) (SEQ ID NO:972):

1. An isolated chimeric polypeptide encoding for R11723_PEA_(—)1_P7 (SEQ ID NO: 144), comprising a first amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence MWVLG (SEQ ID NO: 1129) corresponding to amino acids 1-5 of R11723_PEA_(—)1_P7 (SEQ ID NO: 144), second amino acid sequence being at least 90% homologous to IAATFCGLFLLPGFALQIQCYQCEEFQLNNDCSSPEFIVNCTVNVQDMCQKEVMEQSAG corresponding to amino acids 22-80 of BAC85273 (SEQ ID NO: 972), which also corresponds to amino acids 6-64 of R11723_PEA_(—)1_P7 (SEQ ID NO: 144), and a third amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence SHCVTRLECSGTISAHCNLCLPGSNDHPT (SEQ ID NO: 1128) corresponding to amino acids 65-93 of R11723_PEA_(—)1_P7 (SEQ ID NO: 144), wherein said first, second and third amino acid sequences are contiguous and in a sequential order.

2. An isolated polypeptide encoding for a head of R11723_PEA_(—)1_P7 (SEQ ID NO: 144), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence MWVLG (SEQ ID NO: 1129) of R11723_PEA_(—)1_P7 (SEQ ID NO: 144).

3. An isolated polypeptide encoding for a tail of R11723_PEA_(—)1_P7 (SEQ ID NO: 144), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence SHCVTRLECSGTISAHCNLCLPGSNDHPT (SEQ ID NO: 1128) in R11723_PEA_(—)1_P7 (SEQ ID NO: 144).

Comparison report between R11723_PEA_(—)1_P7 (SEQ ID NO: 144) and BAC85518 (SEQ ID NO: 971):

1. An isolated chimeric polypeptide encoding for R11723_PEA_(—)1_P7 (SEQ ID NO: 144), comprising a first amino acid sequence being at least 90% homologous to MWVLGIAATFCGLFLLPGFALQIQCYQCEEFQLNNDCSSPEFIVNCTVNVQDMCQKEV MEQSAG corresponding to amino acids 24-87 of BAC85518 (SEQ ID NO: 971), which also corresponds to amino acids 1-64 of R11723_PEA_(—)1_P7 (SEQ ID NO: 144), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence SHCVTRLECSGTISAHCNLCLPGSNDHPT (SEQ ID NO: 1128) corresponding to amino acids 65-93 of R11723_PEA_(—)1_P7 (SEQ ID NO: 144), wherein said first and second amino acid sequences are contiguous and in a sequential order.

2. An isolated polypeptide encoding for a tail of R11723_PEA_(—)1_P7 (SEQ ID NO: 144), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence SHCVTRLECSGTISAHCNLCLPGSNDHPT (SEQ ID NO: 1128) in R11723_PEA_(—)1_P7 (SEQ ID NO: 144).

The location of the variant protein was determined according to results from a number of different software programs and analyses, including analyses from SignalP and other specialized programs. The variant protein is believed to be located as follows with regard to the cell: secreted. The protein localization is believed to be secreted because both signal-peptide prediction programs predict that this protein has a signal peptide, and neither trans-membrane region prediction program predicts that this protein has a trans-membrane region.

Variant protein R11723_PEA_(—)1_P7 (SEQ ID NO: 144) also has the following non-silent SNPs (Single Nucleotide Polymorphisms) as listed in Table 10, (given according to their position(s) on the amino acid sequence, with the alternative amino acid(s) listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein R11723_PEA_(—)1_P7 (SEQ ID NO: 144) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 10 Amino acid mutations SNP position(s) on amino acid Alternative amino Previously known sequence acid(s) SNP? 67 C -> S Yes

Variant protein R11723_PEA_(—)1_P7 (SEQ ID NO: 144) is encoded by the following transcript(s): R11723_PEA_(—)1_T17 (SEQ ID NO: 11), for which the sequence(s) is/are given at the end of the application. The coding portion of transcript R11723_PEA_(—)1_T17 (SEQ ID NO: 111) is shown in bold; this coding portion starts at position 434 and ends at position 712. The transcript also has the following SNPs as listed in Table 11 (given according to their position on the nucleotide sequence, with the alternative nucleic acid listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein R11723_PEA_(—)1_P7 (SEQ ID NO: 144) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 11 Nucleic acid SNPs SNP position on nucleotide Previously known sequence Alternative nucleic acid SNP? 625 G -> T Yes 633 G -> C Yes 1303 C -> T Yes

Variant protein R11723_PEA_(—)1_P13 (SEQ ID NO: 145) according to the present invention has an amino acid sequence as given at the end of the application; it is encoded by transcript(s) 19 and R11723_PEA_(—)1_T5 (SEQ ID NO: 114). One or more alignments to one or more previously published protein sequences are given at the end of the application. A brief description of the relationship of the variant protein according to the present invention to each such aligned protein is as follows:

Comparison report between R11723_PEA_(—)1_P13 (SEQ ID NO: 145) and Q96AC2 (SEQ ID NO: 969):

1. An isolated chimeric polypeptide encoding for R11723_PEA_(—)1_P13 (SEQ ID NO: 145), comprising a first amino acid sequence being at least 90% homologous to MWVLGIAATFCGLFLLPGFALQIQCYQCEEFQLNNDCSSPEFIVNCTVNVQDMCQKEV MEQSA corresponding to amino acids 1-63 of Q96AC2 (SEQ ID NO: 969), which also corresponds to amino acids 1-63 of R11723_PEA_(—)1_P13 (SEQ ID NO: 145), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence DTKRTNTLLFEMRHFAKQLTT (SEQ ID NO: 1130) corresponding to amino acids 64-84 of R11723_PEA_(—)1_P13 (SEQ ID NO: 145), wherein said first and second amino acid sequences are contiguous and in a sequential order.

2. An isolated polypeptide encoding for a tail of R11723_PEA_(—)1_P13 (SEQ ID NO: 145), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence DTKRTNTLLFEMRHFAKQLTT (SEQ ID NO: 1130) in R11723_PEA_(—)1_P13 (SEQ ID NO: 145).

The location of the variant protein was determined according to results from a number of different software programs and analyses, including analyses from SignalP and other specialized programs. The variant protein is believed to be located as follows with regard to the cell: secreted. The protein localization is believed to be secreted because both signal-peptide prediction programs predict that this protein has a signal peptide, and neither trans-membrane region prediction program predicts that this protein has a trans-membrane region.

Variant protein R11723_PEA_(—)1_P13 (SEQ ID NO: 145) is encoded by the following transcript(s): R11723_PEA_(—)1_T19 (SEQ ID NO: 112), for which the sequence(s) is/are given at the end of the application. The coding portion of transcript R11723_PEA_(—)1_T19 (SEQ ID NO: 112) is shown in bold; this coding portion starts at position 434 and ends at position 685. The transcript also has the following SNPs as listed in Table 12 (given according to their position on the nucleotide sequence, with the alternative nucleic acid listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein R11723_PEA_(—)1_P13 (SEQ ID NO: 145) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 12 Nucleic acid SNPs SNP position on nucleotide Previously known sequence Alternative nucleic acid SNP? 778 G -> T Yes 786 G -> C Yes 1456 C -> T Yes

Variant protein R11723_PEA_(—)1_P10 (SEQ ID NO: 146) according to the present invention has an amino acid sequence as given at the end of the application; it is encoded by transcript(s) R11723_PEA_(—)1_T20 (SEQ ID NO: 113). One or more alignments to one or more previously published protein sequences are given at the end of the application. A brief description of the relationship of the variant protein according to the present invention to each such aligned protein is as follows:

Comparison report between R11723_PEA_(—)1_P10 (SEQ ID NO: 146) and Q96AC2 (SEQ ID NO: 969):

1. An isolated chimeric polypeptide encoding for R11723_PEA_(—)1_P10 (SEQ ID NO: 146), comprising a first amino acid sequence being at least 90% homologous to MWVLGIAATFCGLFLLPGFALQIQCYQCEEFQLNNDCSSPEFIVNCTVNVQDMCQKEV MEQSA corresponding to amino acids 1-63 of Q96AC2 (SEQ ID NO: 969), which also corresponds to amino acids 1-63 of R11723_PEA_(—)1_P10 (SEQ ID NO: 146), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence DRVSLCHEAGVQWNNFSTLQPLPPRLK (SEQ ID NO: 1131) corresponding to amino acids 64-90 of R11723_PEA_(—)1_P10 (SEQ ID NO: 146), wherein said first and second amino acid sequences are contiguous and in a sequential order.

2. An isolated polypeptide encoding for a tail of R11723_PEA_(—)1_P10 (SEQ ID NO: 146), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence DRVSLCHEAGVQWNNFSTLQPLPPRLK (SEQ ID NO: 1131) in R11723_PEA_(—)1_P10 (SEQ ID NO: 146).

Comparison report between R11723_PEA_(—)1_P10 (SEQ ID NO: 146) and Q8N2G4 (SEQ ID NO: 970):

1. An isolated chimeric polypeptide encoding for R11723_PEA_(—)1_P10 (SEQ ID NO: 146), comprising a first amino acid sequence being at least 90% homologous to MWVLGIAATFCGLFLLPGFALQIQCYQCEEFQLNNDCSSPEFIVNCTVNVQDMCQKEV MEQSA corresponding to amino acids 1-63 of Q8N2G4 (SEQ ID NO: 970), which also corresponds to amino acids 1-63 of R11723_PEA_(—)1_P10 (SEQ ID NO: 146), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence DRVSLCHEAGVQWNNFSTLQPLPPRLK (SEQ ID NO: 1131) corresponding to amino acids 64-90 of R11723_PEA_(—)1_P10 (SEQ ID NO: 146), wherein said first and second amino acid sequences are contiguous and in a sequential order.

2. An isolated polypeptide encoding for a tail of R11723_PEA_(—)1_P10 (SEQ ID NO: 146), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence DRVSLCHEAGVQWNNFSTLQPLPPRLK (SEQ ID NO: 1131) in R11723_PEA_(—)1_P10 (SEQ ID NO: 146).

Comparison report between R11723_PEA_(—)1_P10 (SEQ ID NO: 146) and BAC85273 (SEQ ID NO: 972):

1. An isolated chimeric polypeptide encoding for R11723_PEA_(—)1_P10 (SEQ ID NO: 146), comprising a first amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence MWVLG (SEQ ID NO: 1129) corresponding to amino acids 1-5 of R11723_PEA_(—)1_P10 (SEQ ID NO: 146), second amino acid sequence being at least 90% homologous to IAATFCGLFLLPGFALQIQCYQCEEFQLNNDCSSPEFIVNCTVNVQDMCQKEVMEQSA corresponding to amino acids 22-79 of BAC85273 (SEQ ID NO: 972), which also corresponds to amino acids 6-63 of R11723_PEA_(—)1_P10 (SEQ ID NO: 146), and a third amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence DRVSLCHEAGVQWNNFSTLQPLPPRLK (SEQ ID NO: 1131) corresponding to amino acids 64-90 of R11723_PEA_(—)1_P10 (SEQ ID NO: 146), wherein said first, second and third amino acid sequences are contiguous and in a sequential order.

2. An isolated polypeptide encoding for a head of R11723_PEA_(—)1_P10 (SEQ ID NO: 146), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence MWVLG (SEQ ID NO: 1129) of R11723_PEA_(—)1_P10 (SEQ ID NO: 146).

3. An isolated polypeptide encoding for a tail of R11723_PEA_(—)1_P10 (SEQ ID NO: 146), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence DRVSLCHEAGVQWNNFSTLQPLPPRLK (SEQ ID NO: 1131) in R11723_PEA_(—)1_P10 (SEQ ID NO: 146).

Comparison report between R11723_PEA_(—)1_P10 (SEQ ID NO: 146) and BAC85518 (SEQ ID NO: 971):

1. An isolated chimeric polypeptide encoding for R11723_PEA_(—)1_P10 (SEQ ID NO: 146), comprising a first amino acid sequence being at least 90% homologous to MWVLGIAATFCGLFLLPGFALQIQCYQCEEFQLNNDCSSPEFIVNCTVNVQDMCQKEV MEQSA corresponding to amino acids 24-86 of BAC85518 (SEQ ID NO: 971), which also corresponds to amino acids 1-63 of R11723_PEA_(—)1_P10 (SEQ ID NO: 146), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence DRVSLCHEAGVQWNNFSTLQPLPPRLK (SEQ ID NO: 1131) corresponding to amino acids 64-90 of R11723_PEA_(—)1_P10 (SEQ ID NO: 146), wherein said first and second amino acid sequences are contiguous and in a sequential order.

2. An isolated polypeptide encoding for a tail of R11723_PEA_(—)1_P10 (SEQ ID NO: 146), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence DRVSLCHEAGVQWNNFSTLQPLPPRLK (SEQ ID NO: 1131) in R11723_PEA_(—)1_P10 (SEQ ID NO: 146).

The location of the variant protein was determined according to results from a number of different software programs and analyses, including analyses from SignalP and other specialized programs. The variant protein is believed to be located as follows with regard to the cell: secreted. The protein localization is believed to be secreted because both signal-peptide prediction programs predict that this protein has a signal peptide, and neither trans-membrane region prediction program predicts that this protein has a trans-membrane region.

Variant protein R11723_PEA_(—)1_P10 (SEQ ID NO: 146) also has the following non-silent SNPs (Single Nucleotide Polymorphisms) as listed in Table 13, (given according to their position(s) on the amino acid sequence, with the alternative amino acid(s) listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein R11723_PEA_(—)1_P10 (SEQ ID NO: 146) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 13 Amino acid mutations SNP position(s) on amino acid Alternative sequence amino acid(s) Previously known SNP? 66 V -> F Yes

Variant protein R11723_PEA_(—)1_P10 (SEQ ID NO: 146) is encoded by the following transcript(s): R11723_PEA_(—)1_T20 (SEQ ID NO: 113), for which the sequence(s) is/are given at the end of the application. The coding portion of transcript R11723_PEA_(—)1_T20 (SEQ ID NO: 113) is shown in bold; this coding portion starts at position 434 and ends at position 703. The transcript also has the following SNPs as listed in Table 14 (given according to their position on the nucleotide sequence, with the alternative nucleic acid listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein R11723_PEA_(—)1_P10 (SEQ ID NO: 146) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 14 Nucleic acid SNPs SNP position on nucleotide Alternative sequence nucleic acid Previously known SNP? 629 G -> T Yes 637 G -> C Yes 1307 C -> T Yes

As noted above, cluster R11723 features 26 segment(s), which were listed in Table 2 above and for which the sequence(s) are given at the end of the application. These segment(s) are portions of nucleic acid sequence(s) which are described herein separately because they are of particular interest. A description of each segment according to the present invention is now provided.

Segment cluster R11723_PEA_(—)1_node_(—)13 (SEQ ID NO: 116) according to the present invention is supported by 5 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): R11723_PEA_(—)1_T19 (SEQ ID NO: 112), R11723_PEA_(—)1_T5 (SEQ ID NO: 114) and R11723_PEA_(—)1_T6 (SEQ ID NO: 115). Table 15 below describes the starting and ending position of this segment on each transcript.

TABLE 15 Segment location on transcripts Segment Segment Transcript starting ending name position position R11723_PEA_1_T19 624 776 (SEQ ID NO:112) R11723_PEA_1_T5 624 776 (SEQ ID NO:114) R11723_PEA_1_T6 658 810 (SEQ ID NO:115)

Segment cluster R11723_PEA_(—)1_node_(—)16 (SEQ ID NO: 117) according to the present invention is supported by 3 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): R11723_PEA_(—)1_T17 (SEQ ID NO: 111), R11723_PEA_(—)1_T19 (SEQ ID NO: 112) and R11723_PEA_(—)1_T20 (SEQ ID NO: 113). Table 16 below describes the starting and ending position of this segment on each transcript.

TABLE 16 Segment location on transcripts Segment Segment Transcript starting ending name position position R11723_PEA_1_T17 624 1367 (SEQ ID NO:111) R11723_PEA_1_T19 777 1520 (SEQ ID NO:112) R11723_PEA_1_T20 628 1371 (SEQ ID NO:113)

Segment cluster R11723_PEA_(—)1_node_(—)19 (SEQ ID NO: 118) according to the present invention is supported by 45 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): R11723_PEA_(—)1_T5 (SEQ ID NO: 114) and R11723_PEA_(—)1_T6 (SEQ ID NO: 115). Table 17 below describes the starting and ending position of this segment on each transcript.

TABLE 17 Segment location on transcripts Segment Segment Transcript starting ending name position position R11723_PEA_1_T5 835 1008 (SEQ ID NO:114) R11723_PEA_1_T6 869 1042 (SEQ ID NO:115)

Segment cluster R11723_PEA_(—)1_node_(—)2 (SEQ ID NO: 119) according to the present invention is supported by 29 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): R11723_PEA_(—)1_T15 (SEQ ID NO: 110), R11723_PEA_(—)1_T17 (SEQ ID NO: 111), R11723_PEA_(—)1_T19 (SEQ ID NO: 112), R11723_PEA_(—)1_T20 (SEQ ID NO: 113), R11723_PEA_(—)1_T5 (SEQ ID NO: 114) and R11723_PEA_(—)1_T6 (SEQ ID NO: 115). Table 18 below describes the starting and ending position of this segment on each transcript.

TABLE 18 Segment location on transcripts Segment Segment Transcript starting ending name position position R11723_PEA_1_T15 1 309 (SEQ ID NO:110) R11723_PEA_1_T17 1 309 (SEQ ID NO:111) R11723_PEA_1_T19 1 309 (SEQ ID NO:112) R11723_PEA_1_T20 1 309 (SEQ ID NO:113) R11723_PEA_1_T5 1 309 (SEQ ID NO:114) R11723_PEA_1_T6 1 309 (SEQ ID NO:115)

Segment cluster R11723_PEA_(—)1_node_(—)22 (SEQ ID NO: 120) according to the present invention is supported by 65 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): R11723_PEA_(—)1_T5 (SEQ ID NO: 114) and R11723_PEA_(—)1_T6 (SEQ ID NO: 115). Table 19 below describes the starting and ending position of this segment on each transcript.

TABLE 19 Segment location on transcripts Segment Segment Transcript starting ending name position position R11723_PEA_1_T5 1083 1569 (SEQ ID NO:114) R11723_PEA_1_T6 1117 1603 (SEQ ID NO:115)

Segment cluster R11723_PEA_(—)1_node_(—)31 (SEQ ID NO: 121) according to the present invention is supported by 70 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): R11723_PEA_(—)1_T15 (SEQ ID NO: 110), R11723_PEA_(—)1_T5 (SEQ ID NO: 114) and R11723_PEA_(—)1_T6 (SEQ ID NO: 115). Table 20 below describes the starting and ending position of this segment on each transcript (it should be noted that these transcripts show alternative polyadenylation).

TABLE 20 Segment location on transcripts Segment Segment Transcript starting ending name position position R11723_PEA_1_T15 1060 1295 (SEQ ID NO:110) R11723_PEA_1_T5 1978 2213 (SEQ ID NO:114) R11723_PEA_1_T6 2012 2247 (SEQ ID NO:115)

According to an optional embodiment of the present invention, short segments related to the above cluster are also provided. These segments are up to about 120 bp in length, and so are included in a separate description.

Segment cluster R11723_PEA_(—)1_node_(—)10 (SEQ ID NO. 122) according to the present invention is supported by 38 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): R11723_PEA_(—)1_T15 (SEQ ID NO: 110), R11723_PEA_(—)1_T17 (SEQ ID NO: 111), R11723_PEA_(—)1_T19 (SEQ ID NO: 112), R11723_PEA_(—)1_T20 (SEQ ID NO: 113), R11723_PEA_(—)1_T5 (SEQ ID NO: 114) and R11723_PEA_(—)1_T6 (SEQ ID NO: 115). Table 21 below describes the starting and ending position of this segment on each transcript.

TABLE 21 Segment location on transcripts Segment Segment Transcript starting ending name position position R11723_PEA_1_T15 486 529 (SEQ ID NO:110) R11723_PEA_1_T17 486 529 (SEQ ID NO:111) R11723_PEA_1_T19 486 529 (SEQ ID NO:112) R11723_PEA_1_T20 486 529 (SEQ ID NO:113) R11723_PEA_1_T5 486 529 (SEQ ID NO:114) R11723_PEA_1_T6 486 529 (SEQ ID NO:115)

Segment cluster R11723_PEA_(—)1_node_(—)11 (SEQ ID NO: 123) according to the present invention is supported by 42 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): R11723_PEA_(—)1_T15 (SEQ ID NO: 110), R11723_PEA_(—)1_T17 (SEQ ID NO: 111), R11723_PEA_(—)1_T19 (SEQ ID NO: 112), R11723_PEA_(—)1_T20 (SEQ ID NO: 113), R11723_PEA_(—)1_T5 (SEQ ID NO: 114) and R11723_PEA_(—)1_T6 (SEQ ID NO: 115). Table 22 below describes the starting and ending position of this segment on each transcript.

TABLE 22 Segment location on transcripts Segment Segment Transcript starting ending name position position R11723_PEA_1_T15 530 623 (SEQ ID NO:110) R11723_PEA_1_T17 530 623 (SEQ ID NO:111) R11723_PEA_1_T19 530 623 (SEQ ID NO:112) R11723_PEA_1_T20 530 623 (SEQ ID NO:113) R11723_PEA_1_T5 530 623 (SEQ ID NO:114) R11723_PEA_1_T6 564 657 (SEQ ID NO:115)

Segment cluster R11723_PEA_(—)1_node_(—)15 (SEQ ID NO: 124) according to the present invention can be found in the following transcript(s): R11723_PEA_(—)1_T20 (SEQ ID NO: 113). Table 23 below describes the starting and ending position of this segment on each transcript.

TABLE 23 Segment location on transcripts Segment Segment Transcript starting ending name position position R11723_PEA_1_T20 624 627 (SEQ ID NO:113)

Segment cluster R11723_PEA_(—)1_node_(—)18 (SEQ ID NO: 125) according to the present invention is supported by 40 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): R11723_PEA_(—)1_T15 (SEQ ID NO: 110), R11723_PEA_(—)1_T5 (SEQ ID NO: 114) and R11723_PEA_(—)1_T6 (SEQ ID NO: 115). Table 24 below describes the starting and ending position of this segment on each transcript.

TABLE 24 Segment location on transcripts Segment Segment Transcript starting ending name position position R11723_PEA_1_T15 624 681 (SEQ ID NO:110) R11723_PEA_1_T5 777 834 (SEQ ID NO:114) R11723_PEA_1_T6 811 868 (SEQ ID NO:115)

Segment cluster R11723_PEA_(—)1_node_(—)20 (SEQ ID NO: 126) according to the present invention can be found in the following transcript(s): R11723_PEA_(—)1_T5 (SEQ ID NO: 114) and R11723_PEA_(—)1_T6 (SEQ ID NO: 115). Table 25 below describes the starting and ending position of this segment on each transcript.

TABLE 25 Segment location on transcripts Segment Segment Transcript starting ending name position position R11723_PEA_1_T5 1009 1019 (SEQ ID NO:114) R11723_PEA_1_T6 1043 1053 (SEQ ID NO:115)

Segment cluster R11723_PEA_(—)1_node_(—)21 (SEQ ID NO: 127) according to the present invention is supported by 36 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): R11723_PEA_(—)1_T5 (SEQ ID NO: 114) and R11723_PEA_(—)1_T6 (SEQ ID NO: 115). Table 26 below describes the starting and ending position of this segment on each transcript.

TABLE 26 Segment location on transcripts Segment Segment Transcript starting ending name position position R11723_PEA_1_T5 1020 1082 (SEQ ID NO:114) R11723_PEA_1_T6 1054 1116 (SEQ ID NO:115)

Segment cluster R11723_PEA_(—)1_node_(—)23 (SEQ ID NO: 128) according to the present invention is supported by 39 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): R11723_PEA_(—)1_T5 (SEQ ID NO: 114) and R11723_PEA_(—)1_T6 (SEQ ID NO: 115). Table 27 below describes the starting and ending position of this segment on each transcript.

TABLE 27 Segment location on transcripts Segment Segment Transcript starting ending name position position R11723_PEA_1_T5 1570 1599 (SEQ ID NO:114) R11723_PEA_1_T6 1604 1633 (SEQ ID NO:115)

Segment cluster R11723_PEA_(—)1_node_(—)24 (SEQ ID NO: 129) according to the present invention is supported by 51 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): R11723_PEA_(—)1_T15 (SEQ ID NO: 110), R11723_PEA_(—)1_T5 (SEQ ID NO: 114) and R11723_PEA_(—)1_T6 (SEQ ID NO: 115). Table 28 below describes the starting and ending position of this segment on each transcript.

TABLE 28 Segment location on transcripts Segment Segment Transcript starting ending name position position R11723_PEA_1_T15 682 765 (SEQ ID NO:110) R11723_PEA_1_T5 1600 1683 (SEQ ID NO:114) R11723_PEA_1_T6 1634 1717 (SEQ ID NO:115)

Segment cluster R11723_PEA_(—)1_node_(—)25 (SEQ ID NO: 130) according to the present invention is supported by 54 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): R11723_PEA_(—)1_T15 (SEQ ID NO: 110), R11723_PEA_(—)1_T5 (SEQ ID NO: 114) and R11723_PEA_(—)1_T6 (SEQ ID NO: 115). Table 29 below describes the starting and ending position of this segment on each transcript.

TABLE 29 Segment location on transcripts Segment Segment Transcript starting ending name position position R11723_PEA_1_T15 766 791 (SEQ ID NO:110) R11723_PEA_1_T5 1684 1709 (SEQ ID NO:114) R11723_PEA_1_T6 1718 1743 (SEQ ID NO:115)

Segment cluster R11723_PEA_(—)1_node_(—)26 (SEQ ID NO: 131) according to the present invention is supported by 62 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): R11723_PEA_(—)1_T15 (SEQ ID NO: 110), R11723_PEA_(—)1_T5 (SEQ ID NO: 114) and R11723_PEA_(—)1_T6 (SEQ ID NO: 115). Table 30 below describes the starting and ending position of this segment on each transcript.

TABLE 30 Segment location on transcripts Segment Segment Transcript starting ending name position position R11723_PEA_1_T15 792 904 (SEQ ID NO:110) R11723_PEA_1_T5 1710 1822 (SEQ ID NO:114) R11723_PEA_1_T6 1744 1856 (SEQ ID NO:115)

Segment cluster R11723_PEA_(—)1_node_(—)27 (SEQ ID NO: 132) according to the present invention is supported by 67 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): R11723_PEA_(—)1_T15 (SEQ ID NO: 110), R11723_PEA_(—)1_T5 (SEQ ID NO: 114) and R11723_PEA_(—)1_T6 (SEQ ID NO: 115). Table 31 below describes the starting and ending position of this segment on each transcript.

TABLE 31 Segment location on transcripts Segment Segment Transcript starting ending name position position R11723_PEA_1_T15 905 986 (SEQ ID NO:110) R11723_PEA_1_T5 1823 1904 (SEQ ID NO:114) R11723_PEA_1_T6 1857 1938 (SEQ ID NO:115)

Segment cluster R11723_PEA_(—)1_node_(—)28 (SEQ ID NO: 133) according to the present invention can be found in the following transcript(s): R11723_PEA_(—)1_T15 (SEQ ID NO: 110), R11723_PEA_(—)1_T5 (SEQ ID NO: 114) and R11723_PEA_(—)1_T6 (SEQ ID NO: 115). Table 32 below describes the starting and ending position of this segment on each transcript.

TABLE 32 Segment location on transcripts Segment Segment Transcript starting ending name position position R11723_PEA_1_T15 987 1010 (SEQ ID NO:110) R11723_PEA_1_T5 1905 1928 (SEQ ID NO:114) R11723_PEA_1_T6 1939 1962 (SEQ ID NO:115)

Segment cluster R11723_PEA_(—)1_node_(—)29 (SEQ ID NO: 134) according to the present invention is supported by 69 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): R11723_PEA_(—)1_T15 (SEQ ID NO: 110), R11723_PEA_(—)1_T5 (SEQ ID NO: 114) and R11723_PEA_(—)1_T6 (SEQ ID NO: 115). Table 33 below describes the starting and ending position of this segment on each transcript.

TABLE 33 Segment location on transcripts Segment Segment Transcript starting ending name position position R11723_PEA_1_T15 1011 1038 (SEQ ID NO:110) R11723_PEA_1_T5 1929 1956 (SEQ ID NO:114) R11723_PEA_1_T6 1963 1990 (SEQ ID NO:115)

Segment cluster R11723_PEA_(—)1_node_(—)3 (SEQ ID NO: 135) according to the present invention can be found in the following transcript(s): R11723_PEA_(—)1_T15 (SEQ ID NO: 110), R11723_PEA_(—)1_T17 (SEQ ID NO: 111), R11723_PEA_(—)1_T19 (SEQ ID NO: 112), R11723_PEA_(—)1_T20 (SEQ ID NO: 113), R11723_PEA_(—)1_T5 (SEQ ID NO: 114) and R11723_PEA_(—)1_T6 (SEQ ID NO: 115). Table 34 below describes the starting and ending position of this segment on each transcript.

TABLE 34 Segment location on transcripts Segment Segment Transcript starting ending name position position R11723_PEA_1_T15 310 319 (SEQ ID NO:110) R11723_PEA_1_T17 310 319 (SEQ ID NO:111) R11723_PEA_1_T19 310 319 (SEQ ID NO:112) R11723_PEA_1_T20 310 319 (SEQ ID NO:113) R11723_PEA_1_T5 310 319 (SEQ ID NO:114) R11723_PEA_1_T6 310 319 (SEQ ID NO:115)

Segment cluster R11723_PEA_(—)1_node_(—)30 (SEQ ID NO: 136) according to the present invention can be found in the following transcript(s): R11723_PEA_(—)1_T15 (SEQ ID NO: 110), R11723_PEA_(—)1_T5 (SEQ ID NO: 114) and R11723_PEA_(—)1_T6 (SEQ ID NO: 115). Table 35 below describes the starting and ending position of this segment on each transcript.

TABLE 35 Segment location on transcripts Segment Segment Transcript starting ending name position position R11723_PEA_1_T15 1039 1059 (SEQ ID NO:110) R11723_PEA_1_T5 1957 1977 (SEQ ID NO:114) R11723_PEA_1_T6 1991 2011 (SEQ ID NO:115)

Segment cluster R11723_PEA_(—)1_node_(—)4 (SEQ ID NO: 137) according to the present invention is supported by 25 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): R11723_PEA_(—)1_T15 (SEQ ID NO: 110), R11723_PEA_(—)1_T17 (SEQ ID NO: 111), R11723_PEA_(—)1_T19 (SEQ ID NO: 112), R11723_PEA_(—)1_T20 (SEQ ID NO: 113), R11723_PEA_(—)1_T5 (SEQ ID NO: 114) and R11723_PEA_(—)1_T6 (SEQ ID NO: 115). Table 36 below describes the starting and ending position of this segment on each transcript.

TABLE 36 Segment location on transcripts Segment Segment Transcript starting ending name position position R11723_PEA_1_T15 320 371 (SEQ ID NO:110) R11723_PEA_1_T17 320 371 (SEQ ID NO:111) R11723_PEA_1_T19 320 371 (SEQ ID NO:112) R11723_PEA_1_T20 320 371 (SEQ ID NO:113) R11723_PEA_1_T5 320 371 (SEQ ID NO:114) R11723_PEA_1_T6 320 371 (SEQ ID NO:115)

Segment cluster R11723_PEA_(—)1_node_(—)5 (SEQ ID NO: 138) according to the present invention is supported by 26 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): R11723_PEA_(—)1_T15 (SEQ ID NO: 110), R11723_PEA_(—)1_T17 (SEQ ID NO: 111), R11723_PEA_(—)1_T19 (SEQ ID NO: 112), R11723_PEA_(—)1_T20 (SEQ ID NO: 113), R11723_PEA_(—)1_T5 (SEQ ID NO: 114) and R11723_PEA_(—)1_T6 (SEQ ID NO: 115). Table 37 below describes the starting and ending position of this segment on each transcript.

TABLE 37 Segment location on transcripts Segment Segment Transcript starting ending name position position R11723_PEA_1_T15 372 414 (SEQ ID NO:110) R11723_PEA_1_T17 372 414 (SEQ ID NO:111) R11723_PEA_1_T19 372 414 (SEQ ID NO:112) R11723_PEA_1_T20 372 414 (SEQ ID NO:113) R11723_PEA_1_T5 372 414 (SEQ ID NO:114) R11723_PEA_1_T6 372 414 (SEQ ID NO:115)

Segment cluster R11723_PEA_(—)1_node_(—)6 (SEQ ID NO: 139) according to the present invention is supported by 27 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): R11723_PEA_(—)1_T15 (SEQ ID NO: 110), R11723_PEA_(—)1_T17 (SEQ ID NO: 111), R11723_PEA_(—)1_T19 (SEQ ID NO: 112), R11723_PEA_(—)1_T20 (SEQ ID NO: 113), R11723_PEA_(—)1_T5 (SEQ ID NO: 114) and R11723_PEA_(—)1_T6 (SEQ ID NO: 115). Table 38 below describes the starting and ending position of this segment on each transcript.

TABLE 38 Segment location on transcripts Segment Segment Transcript starting ending name position position R11723_PEA_1_T15 415 446 (SEQ ID NO:110) R11723_PEA_1_T17 415 446 (SEQ ID NO:111) R11723_PEA_1_T19 415 446 (SEQ ID NO:112) R11723_PEA_1_T20 415 446 (SEQ ID NO:113) R11723_PEA_1_T5 415 446 (SEQ ID NO:114) R11723_PEA_1_T6 415 446 (SEQ ID NO:115)

Segment cluster R11723_PEA_(—)1_node_(—)7 (SEQ ID NO: 140) according to the present invention is supported by 29 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): R11723_PEA_(—)1_T15 (SEQ ID NO: 110), R11723_PEA_(—)1_T17 (SEQ ID NO: 111), R11723_PEA_(—)1_T19 (SEQ ID NO: 112), R11723_PEA_(—)1_T20 (SEQ ID NO: 113), R11723_PEA_(—)1_T5 (SEQ ID NO: 114) and R11723_PEA_(—)1_T6 (SEQ ID NO: 115). Table 39 below describes the starting and ending position of this segment on each transcript.

TABLE 39 Segment location on transcripts Segment Segment Transcript starting ending name position position R11723_PEA_1_T15 447 485 (SEQ ID NO:110) R11723_PEA_1_T17 447 485 (SEQ ID NO:111) R11723_PEA_1_T19 447 485 (SEQ ID NO:112) R11723_PEA_1_T20 447 485 (SEQ ID NO:113) R11723_PEA_1_T5 447 485 (SEQ ID NO:114) R11723_PEA_1_T6 447 485 (SEQ ID NO:115)

Segment cluster R11723_PEA_(—)1_node_(—)8 (SEQ ID NO: 141) according to the present invention is supported by 2 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): R11723_PEA_(—)1_T6 (SEQ ID NO: 115). Table 40 below describes the starting and ending position of this segment on each transcript.

TABLE 40 Segment location on transcripts Segment Segment Transcript starting ending name position position R11723_PEA_1_T6 486 519 (SEQ ID NO:115) Variant protein alignment to the previously known protein: Sequence name: /tmp/gp6eQTLWqk/mFtjUpUzhb:Q8IXM0 (SEQ ID NO: 968) Sequence documentation:

Alignment of: R11723_PEA_(—)1_P6 (SEQ ID NO: 143) x Q8IXM0 (SEQ ID NO: 968) ..

Alignment segment 1/1:

Quality: 1128.00 Escore: 0 Matching length: 112 Total length: 112 Matching Percent Similarity: 100.00 Matching Percent Identity: 100.00 Total Percent Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0

Alignment:

Sequence name: /tmp/gp6eQTLWqk/mFtjUpUzhb:Q96AC2 (SEQ ID NO: 969) Sequence documentation:

Alignment of: R11723_PEA_(—)1_P6 (SEQ ID NO: 143) x Q96AC2 (SEQ ID NO: 969) ..

Alignment segment 1/1:

Quality: 835.00 Escore: 0 Matching length: 83 Total length: 83 Matching Percent Similarity: 100.00 Matching Percent Identity: 100.00 Total Percent Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0

Alignment:

Sequence name: /tmp/gp6eQTLWqk/mFtjUpUzhb:Q8N2G4 (SEQ ID NO: 970) Sequence documentation:

Alignment of: R11723_PEA_(—)1_P6 (SEQ ID NO: 143) x Q8N2G4 (SEQ ID NO: 970) ..

Alignment segment 1/1:

Quality: 835.00 Escore: 0 Matching length: 83 Total length: 83 Matching Percent Similarity: 100.00 Matching Percent Identity: 100.00 Total Percent Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0

Alignment:

Sequence name: /tmp/gp6eQTLWqk/mFtjUpUzhb:BAC85518 (SEQ ID NO: 971) Sequence documentation:

Alignment of: R11723_PEA_(—)1_P6 (SEQ ID NO: 143) x BAC85518 (SEQ ID NO: 971) ..

Alignment segment 1/1:

Quality: 835.00 Escore: 0 Matching length: 83 Total length: 83 Matching Percent Similarity: 100.00 Matching Percent Identity: 100.00 Total Percent Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0

Alignment:

Sequence name: /tmp/VXjdFlzdBX/bexTxTh0Th:Q96AC2 (SEQ ID NO: 969) Sequence documentation:

Alignment of: R11723_PEA_(—)1_P7 (SEQ ID NO: 144) x Q96AC2 (SEQ ID NO: 969) ..

Alignment segment 1/1:

Quality: 654.00 Escore: 0 Matching length: 64 Total length: 64 Matching Percent Similarity: 100.00 Matching Percent Identity: 100.00 Total Percent Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0

Alignment:

Sequence name: /tmp/VXjdFlzdBX/bexTxTh0Th:Q8N2G4 (SEQ ID NO: 970) Sequence documentation:

Alignment of: R11723_PEA_(—)1_P7 (SEQ ID NO: 144) x Q8N2G4 (SEQ ID NO: 970) ..

Alignment segment 1/1:

Quality: 654.00 Escore: 0 Matching length: 64 Total length: 64 Matching Percent Similarity: 100.00 Matching Percent Identity: 100.00 Total Percent Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0

Alignment:

Sequence name: /tmp/VXjdFlzdBX/bexTxTh0Th:BAC85273 (SEQ ID NO: 972) Sequence documentation:

Alignment of: R11723_PEA_(—)1_P7 (SEQ ID NO: 144) x BAC85273 (SEQ ID NO: 972) ..

Alignment segment 1/1:

Quality: 600.00 Escore: 0 Matching length: 59 Total length: 59 Matching Percent Similarity: 100.00 Matching Percent Identity: 100.00 Total Percent Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0

Alignment:

Sequence name: /tmp/VXjdFlzdBX/bexTxTh0Th:BAC85518 (SEQ ID NO: 971) Sequence documentation:

Alignment of: R11723_PEA_(—)1_P7 (SEQ ID NO: 144) x BAC85518 (SEQ ID NO: 971) ..

Alignment segment 1/1:

Quality: 654.00 Escore: 0 Matching length: 64 Total length: 64 Matching Percent Similarity: 100.00 Matching Percent Identity: 100.00 Total Percent Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0

Alignment:

Sequence name: /tmp/OLMSexEmIh/pc7Z7Xm1YR:Q96AC2 (SEQ ID NO: 969) Sequence documentation:

Alignment of: R11723_PEA_(—)1_P10 (SEQ ID NO: 146) x Q96AC2 (SEQ ID NO: 969) ..

Alignment segment 1/1:

Quality: 645.00 Escore: 0 Matching length: 63 Total length: 63 Matching Percent Similarity: 100.00 Matching Percent Identity: 100.00 Total Percent Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0

Alignment:

Sequence name: /tmp/OLMSexEmIh/pc7Z7Xm1YR:Q8N2G4 (SEQ ID NO: 970) Sequence documentation:

Alignment of: R11723_PEA_(—)1_P10 (SEQ ID NO: 146) x Q8N2G4 (SEQ ID NO: 970) ..

Alignment segment 1/1:

Quality: 645.00 Escore: 0 Matching length: 63 Total length: 63 Matching Percent Similarity: 100.00 Matching Percent Identity: 100.00 Total Percent Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0

Alignment:

Sequence name: /tmp/OLMSexEmIh/pc7Z7Xm1YR:BAC85273 (SEQ ID NO: 972) Sequence documentation:

Alignment of: R11723_PEA_(—)1_P10 (SEQ ID NO: 146) x BAC85273 (SEQ ID NO: 972) ..

Alignment segment 1/1:

Quality: 591.00 Escore: 0 Matching length: 58 Total length: 58 Matching Percent Similarity: 100.00 Matching Percent Identity: 100.00 Total Percent Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0

Alignment:

Sequence name: /tmp/OLMSexEmIh/pc7Z7Xm1YR:BAC85518 (SEQ ID NO: 971) Sequence documentation:

Alignment of: R11723_PEA_(—)1_P10 (SEQ ID NO: 146) x BAC85518 (SEQ ID NO: 971) ..

Alignment segment 1/1:

Quality: 645.00 Escore: 0 Matching length: 63 Total length: 63 Matching Percent Similarity: 100.00 Matching Percent Identity: 100.00 Total Percent Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0

Alignment:

Alignment of: R11723_PEA_(—)1_P13 (SEQ ID NO: 145) x Q96AC2 (SEQ ID NO: 969) ..

Alignment segment 1/1:

Quality: 645.00 Escore: 0 Matching length: 63 Total length: 63 Matching Percent Similarity: 100.00 Matching Percent Identity: 100.00 Total Percent Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0

Alignment:

Expression of R11723 transcripts which are detectable by amplicon as depicted in sequence R11723 seg13 (SEQ ID NO:975) in normal and cancerous ovary tissues

Expression of transcripts detectable by or according to seg13, R11723seg13 amplicon(s) (SEQ ID NO:975) and R11723seg13F (SEQ ID NO:973) and R11723seg13R (SEQ ID NO:974) primers was measured by real time PCR. In parallel the expression of four housekeeping genes —PBGD (GenBank Accession No. BC019323, (SEQ ID NO:1036); amplicon—PBGD-amplicon, (SEQ ID NO:1039)), HPRT1 (GenBank Accession No. NM_(—)000194, (SEQ ID NO:1040); amplicon—HPRT1-amplicon, (SEQ ID NO:1043)), SDHA (GenBank Accession No. NM_(—)004168, (SEQ ID NO:1032); amplicon—SDHA-amplicon, (SEQ ID NO:1035)), and GAPDH (GenBank Accession No. BC026907, (SEQ ID NO:1044); GAPDH amplicon, (SEQ ID NO: 1047)) was measured similarly. For each RT sample, the expression of the above amplicon was normalized to the geometric mean of the quantities of the housekeeping genes. The normalized quantity of each RT sample was then divided by the median of the quantities of the normal post-mortem (PM) samples (Sample Nos. 45-48, 71, Table 1, “Tissue samples in testing panel”, above), to obtain a value of fold up-regulation for each sample relative to median of the normal PM samples.

FIG. 16 is a histogram showing over expression of the above-indicated transcripts in cancerous ovary samples relative to the normal PM samples. Values represent the average of duplicate experiments. Error bars indicate the minimal and maximal values obtained.

As is evident from FIG. 16, the expression of transcripts detectable by the above amplicon(s) in cancer samples was significantly higher than in the non-cancerous samples (Sample Nos. 45-48, 71, Table 1, “Tissue samples in testing panel”). Notably an over-expression of at least 5 fold was found in 23 out of 43 adenocarcinoma samples, Statistical analysis was applied to verify the significance of these results, as described below.

The P value for the difference in the expression levels of transcripts detectable by the above amplicon(s) in ovary cancer samples versus the normal tissue samples was determined by T test as 4.76E-04.

Threshold of 5 fold overexpression was found to differentiate between cancer and normal samples with P value of 2.48E-02 as checked by exact fisher test. The above values demonstrate statistical significance of the results.

Primer pairs are also optionally and preferably encompassed within the present invention; for example, for the above experiment, the following primer pair was used as a non-limiting illustrative example only of a suitable primer pair R11723seg1F forward primer; and R11723seg13R (SEQ ID NO:974) reverse primer.

The present invention also preferably encompasses any amplicon obtained through the use of any suitable primer pair; for example, for the above experiment, the following amplicon was obtained as a non-limiting illustrative example only of a suitable amplicon: R11723seg13 (SEQ ID NO:975).

R11723seg13F (SEQ ID NO:973)- ACACTAAAAGAACAAACACCTTGCTC R11723seg13R (SEQ ID NO:974)- TCCTCAGAAGGCACATGAAAGA R11723seg13 (SEQ ID NO:975)- ACACTAAAAGAACAAACACCTTGCTCTTCGAGATGAGACATTTTGCCAAG CAGTTGACCACTTAGTTCTCAAGAAGCAACTATCTCTTTCATGTGCCTTC TGAGGA

Expression of R11723 transcripts which are detectable by amplicon as depicted in sequence name R11723seg13 (SEQ ID NO:975) in different normal tissues

Expression of R11723 transcripts detectable by or according to R11723seg13 amplicon (SEQ ID NO:975) and R11723seg13F (SEQ ID NO:973), R11723seg13R (SEQ ID NO:974) was measured by real time PCR. In parallel the expression of four housekeeping genes RPL19 (GenBank Accession No. NM_(—)000981, (SEQ ID NO:1048); RPL19 amplicon, (SEQ ID NO:1051)), TATA box (GenBank Accession No. NM_(—)003194, (SEQ ID NO:1052); TATA amplicon, (SEQ ID NO:1055)), Ubiquitin (GenBank Accession No. BC000449, (SEQ ID NO: 1056); amplicon—Ubiquitin-amplicon, (SEQ ID NO: 1059)) and SDHA (GenBank Accession No. NM_(—)004168, (SEQ ID NO: 1032); amplicon—SDHA-amplicon, (SEQ ID NO: 1035)) was measured similarly. For each RT sample, the expression of the above amplicon was normalized to the geometric mean of the quantities of the housekeeping genes. The normalized quantity of each RT sample was then divided by the median of the quantities of the ovary samples (Sample Nos. 18-20, Table 2 above, “Tissue samples in normal panel”), to obtain a value of relative expression of each sample relative to median of the ovary samples.

The results are described in FIG. 17, presenting the histogram showing the expression of R11723 transcripts, which are detectable by amplicon as depicted in sequence name R11723seg13 (SEQ ID NO:975), in different normal tissues. Primers and amplicon are as above.

Expression of R11723 transcripts, which are detectable by amplicon as depicted in sequence R11723 junc11-18 (SEQ ID NO:978) in normal and cancerous ovary tissues

Expression of transcripts detectable by or according to junc11-18 R11723 junc11-18 (SEQ ID NO:978) amplicon and R11723 junc11-18F (SEQ ID NO:976) and R1172 junc11-18R (SEQ ID NO:977) primers was measured by real time PCR (It should be noted that the variants of this cluster are variants of the hypothetical protein PSEC0181 (referred to herein as “PSEC”). Furthermore, use of the known protein (WT protein) for detection of ovarian cancer, alone or in combination with one or more variants of this cluster and/or of any other cluster and/or of any known marker, also comprises an embodiment of the present invention). In parallel the expression of four housekeeping genes —PBGD (GenBank Accession No. BC019323, (SEQ ID NO:1036); amplicon—PBGD-amplicon, (SEQ ID NO:1039)), HPRT1 (GenBank Accession No. NM_(—)000194, (SEQ ID NO:1040); amplicon—HPRT1-amplicon, (SEQ ID NO:1043)), SDHA (GenBank Accession No. NM_(—)004168, (SEQ ID NO:1032); amplicon—SDHA-amplicon, (SEQ ID NO:1035)), and GAPDH (GenBank Accession No. BC026907, (SEQ ID NO:1044); GAPDH amplicon, (SEQ ID NO: 1047)) was measured similarly. For each RT sample, the expression of the above amplicon was normalized to the geometric mean of the quantities of the housekeeping genes. The normalized quantity of each RT sample was then divided by the median of the quantities of the normal post-mortem (PM) samples (Sample Nos 45-48, 71, Table 1, above: “Tissue samples in ovarian cancer testing panel”), to obtain a value of fold up-regulation for each sample relative to median of the normal PM samples.

FIG. 18 is a histogram showing over expression of the above-indicated transcripts in cancerous ovary samples relative to the normal samples. Values represent the average of duplicate experiments. Error bars indicate the minimal and maximal values obtained.

As is evident from FIG. 18, the expression of transcripts detectable by the above amplicon in cancer samples was higher than in the non-cancerous samples (Sample Nos 45-48, 71 Table 1, “Tissue samples in ovarian cancer testing panel”). Notably an over-expression of at least 5 fold was found in 23 out of 43 adenocarcinoma samples.

Primer pairs are also optionally and preferably encompassed within the present invention; for example, for the above experiment, the following primer pair was used as a non-limiting illustrative example only of a suitable primer pair R11723 junc11-18F (SEQ ID NO:976) forward primer; and R11723 junc11-18R (SEQ ID NO:977) reverse primer.

The present invention also preferably encompasses any amplicon obtained through the use of any suitable primer pair; for example, for the above experiment, the following amplicon was obtained as a non-limiting illustrative example only of a suitable amplicon: R11723 junc11-18 (SEQ ID NO:978).

R11723junc11-18F (SEQ ID NO:976)- AGTGATGGAGCAAAGTGCCG R11723 junc11-18R (SEQ ID NO:977)- CAGCAGCTGATGCAAACTGAG R11723 junc11-18 (SEQ ID NO:978)- AGTGATGGAGCAAAGTGCCGGGATCATGTACCGCAAGTCCTGTGCATCAT CAGCGGCCTGTCTCATCGCCTCTGCCGGGTACCAGTCCTTCTGCTCCCCA GGGAAACTGAACTCAGTTTGCATCAGCTGCTG

Expression of R11723 transcripts, which are detectable by amplicon as depicted in sequence name R11723 junc11-18 (SEQ ID NO:978) in different normal tissues

Expression of R11723 transcripts detectable by or according to R11723seg13 (SEQ ID NO:978) amplicon and R11723 junc11-18F (SEQ ID NO:976), R11723 junc11-18R (SEQ ID NO:977) was measured by real time PCR. In parallel the expression of four housekeeping genes -RPL19 (GenBank Accession No. NM_(—)000981, (SEQ ID NO:1048); RPL19 amplicon, (SEQ ID NO: 1051)), TATA box (GenBank Accession No. NM_(—)003194, (SEQ ID NO: 1052); TATA amplicon, (SEQ ID NO:1055)), UBC (GenBank Accession No. BC000449, (SEQ ID NO: 1056); amplicon—Ubiquitin-amplicon, (SEQ ID NO: 1059)) and SDHA (GenBank Accession No. NM_(—)004168, (SEQ ID NO:1032); amplicon—SDHA-amplicon, (SEQ ID NO: 1035)) was measured similarly. For each RT sample, the expression of the above amplicon was normalized to the geometric mean of the quantities of the housekeeping genes. The normalized quantity of each RT sample was then divided by the median of the quantities of the ovary samples (Sample Nos. 18-20 Table 2 above: “Tissue samples in normal panel”), to obtain a value of relative expression of each sample relative to median of the ovary samples.

The results are described in FIG. 19, presenting the histogram showing the expression of R11723 transcripts, which are detectable by amplicon as depicted in sequence name R11723 junc11-18 (SEQ ID NO:978), in different normal tissues. Amplicon and primers are as above.

Description for Cluster D56406

Cluster D56406 features 3 transcript(s) and 10 segment(s) of interest, the names for which are given in Tables 1 and 2, respectively, the sequences themselves are given at the end of the application. The selected protein variants are given in table 3.

TABLE 1 Transcripts of interest Transcript Name SEQ ID NO: D56406_PEA_1_T3 147 D56406_PEA_1_T6 148 D56406_PEA_1_T7 149

TABLE 2 Segments of interest Segment Name SEQ ID NO: D56406_PEA_1_node_0 150 D56406_PEA_1_node_13 151 D56406_PEA_1_node_11 152 D56406_PEA_1_node_2 153 D56406_PEA_1_node_3 154 D56406_PEA_1_node_5 155 D56406_PEA_1_node_6 156 D56406_PEA_1_node_7 157 D56406_PEA_1_node_8 158 D56406_PEA_1_node_9 159

TABLE 3 Proteins of interest Protein Name SEQ ID NO: D56406_PEA_1_P2 161 D56406_PEA_1_P5 162 D56406_PEA_1_P6 163

These sequences are variants of the known protein Neurotensin/neuromedin N precursor [Contains: Large neuromedin N (NmN-125); Neuromedin N (NmN) (NN); Neurotensin (NT); Tail peptide] (SwissProt accession identifier NEUT_HUMAN), SEQ ID NO: 160, referred to herein as the previously known protein.

Protein Neurotensin/neuromedin N precursor is known or believed to have the following function(s): Neurotensin may play an endocrine or paracrine role in the regulation of fat metabolism. It causes contraction of smooth muscle. The sequence for protein Neurotensin/neuromedin N precursor is given at the end of the application, as “Neurotensin/neuromedin N precursor [Contains: Large neuromedin N (NmN-125); Neuromedin N (NmN) (NN); Neurotensin (NT); Tail peptide] amino acid sequence”. Protein Neurotensin/neuromedin N precursor localization is believed to be secreted; packaged within secretory vesicles.

The following GO Annotation(s) apply to the previously known protein. The following annotation(s) were found: signal transduction, which are annotation(s) related to Biological Process; neuropeptide hormone, which are annotation(s) related to Molecular Function; and extracellular; soluble fraction, which are annotation(s) related to Cellular Component.

The GO assignment relies on information from one or more of the SwissProt/TremBl Protein knowledgebase, available from <http://www.expasy.ch/sprot/>; or Locuslink, available from <http://www.ncbi.nlm.nih.gov/projects/LocusLink/>.

As noted above, cluster D56406 features 3 transcript(s), which were listed in Table 1 above. These transcript(s) encode for protein(s) which are variant(s) of protein Neurotensin/neuromedin N precursor. A description of each variant protein according to the present invention is now provided.

Variant protein D56406_PEA_(—)1_P2 (SEQ ID NO: 161) according to the present invention has an amino acid sequence as given at the end of the application; it is encoded by transcript(s) D56406_PEA_(—)1_T3 (SEQ ID NO: 147). An alignment is given to the known protein (Neurotensin/neuromedin N precursor) at the end of the application. One or more alignments to one or more previously published protein sequences are given at the end of the application. A brief description of the relationship of the variant protein according to the present invention to each such aligned protein is as follows:

Comparison report between D56406_PEA_(—)1_P2 (SEQ ID NO: 161) and NEUT_HUMAN:

1. An isolated chimeric polypeptide encoding for D56406_PEA_(—)1_P2 (SEQ ID NO: 161), comprising a first amino acid sequence being at least 90% homologous to MMAGMKIQLVCMLLLAFSSWSLCSDSEEEMKALEADFLTNMHTSKISKAHVPSWKMT LLNVCSLVNNLNSPAEETGEVHEEELVARRKLPTALDGFSLEAMLTIYQLHKICHSRAF QHWE corresponding to amino acids 1-120 of NEUT_HUMAN, which also corresponds to amino acids 1-120 of D56406_PEA_(—)1_P2 (SEQ ID NO: 161), second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence ARWLTPVIPALWEAETGGSRGQEMETIPANT (SEQ ID NO: 1141) corresponding to amino acids 121-151 of D56406_PEA_(—)1_P2 (SEQ ID NO: 161), and a third amino acid sequence being at least 90% homologous to LIQEDILDTGNDKNGKEEVIKRKIPYILKRQLYENKPRRPYILKRDSYYY corresponding to amino acids 121-170 of NEUT_HUMAN, which also corresponds to amino acids 152-201 of D56406_PEA_(—)1_P2 (SEQ ID NO: 161), wherein said first, second and third amino acid sequences are contiguous and in a sequential order.

2. An isolated polypeptide encoding for an edge portion of D56406_PEA_(—)1_P2 (SEQ ID NO: 161), comprising an amino acid sequence being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence encoding for ARWLTPVIPALWEAETGGSRGQEMETIPANT (SEQ ID NO: 1141), corresponding to D56406_PEA_(—)1_P2 (SEQ ID NO: 161).

The location of the variant protein was determined according to results from a number of different software programs and analyses, including analyses from SignalP and other specialized programs. The variant protein is believed to be located as follows with regard to the cell: secreted. The protein localization is believed to be secreted because both signal-peptide prediction programs predict that this protein has a signal peptide, and neither trans-membrane region prediction program predicts that this protein has a trans-membrane region.

Variant protein D56406_PEA_(—)1_P2 (SEQ ID NO: 161) also has the following non-silent SNPs (Single Nucleotide Polymorphisms) as listed in Table 4, (given according to their position(s) on the amino acid sequence, with the alternative amino acid(s) listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein D56406_PEA_(—)1_P2 (SEQ ID NO: 161) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 4 Amino acid mutations SNP position(s) on amino acid Alternative sequence amino acid(s) Previously known SNP? 30 M -> V No 44 S -> P No 84 V -> No 84 V -> A No

Variant protein D56406_PEA_(—)1_P2 (SEQ ID NO: 161) is encoded by the following transcript(s): D56406_PEA_(—)1_T3 (SEQ ID NO: 147), for which the sequence(s) is/are given at the end of the application. The coding portion of transcript D56406_PEA_(—)1_T3 (SEQ ID NO: 147) is shown in bold; this coding portion starts at position 106 and ends at position 708. The transcript also has the following SNPs as listed in Table 5 (given according to their position on the nucleotide sequence, with the alternative nucleic acid listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein D56406_PEA_(—)1_P2 (SEQ ID NO: 161) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 5 Nucleic acid SNPs SNP position on nucleotide Alternative sequence nucleic acid Previously known SNP? 94 G -> T No 95 A -> T No 858 T -> G Yes 103 A -> G Yes 193 A -> G No 235 T -> C No 339 T -> C No 356 T -> No 356 T -> C No 417 A -> T No 757 T -> No

Variant protein D56406_PEA_(—)1_P5 (SEQ ID NO: 162) according to the present invention has an amino acid sequence as given at the end of the application; it is encoded by transcript(s) D56406_PEA_(—)1_T6 (SEQ ID NO: 148). An alignment is given to the known protein (Neurotensin/neuromedin N precursor) at the end of the application. One or more alignments to one or more previously published protein sequences are given at the end of the application. A brief description of the relationship of the variant protein according to the present invention to each such aligned protein is as follows:

Comparison report between D56406_PEA_(—)1_P5 (SEQ ID NO: 162) and NEUT_HUMAN:

1. An isolated chimeric polypeptide encoding for D56406_PEA_(—)1_P5 (SEQ ID NO: 162), comprising a first amino acid sequence being at least 90% homologous to MMAGMKIQLVCMLLLAFSSWSLC corresponding to amino acids 1-23 of NEUT_HUMAN, which also corresponds to amino acids 1-23 of D56406_PEA_(—)1_P5 (SEQ ID NO: 162), and a second amino acid sequence being at least 90% homologous to SEEEMKALEADFLTNMHTSKISKAHVPSWKMTLLNVCSLVNNLNSPAEETGEVHEEEL VARRKLPTALDGFSLEAMLTIYQLHKICHSRAFQHWELIQEDILDTGNDKNGKEEVIKR KIPYILKRQLYENKPRRPYILKRDSYYY corresponding to amino acids 26-170 of NEUT_HUMAN, which also corresponds to amino acids 24-168 of D56406_PEA_(—)1_P5 (SEQ ID NO: 162), wherein said first and second amino acid sequences are contiguous and in a sequential order.

2. An isolated chimeric polypeptide encoding for an edge portion of D56406_PEA_(—)1_P5 (SEQ ID NO: 162), comprising a polypeptide having a length “n”, wherein n is at least about 10 amino acids in length, optionally at least about 20 amino acids in length, preferably at least about 30 amino acids in length, more preferably at least about 40 amino acids in length and most preferably at least about 50 amino acids in length, wherein at least two amino acids comprise CS, having a structure as follows: a sequence starting from any of amino acid numbers 23-x to 23; and ending at any of amino acid numbers 24+((n−2)−x), in which x varies from 0 to n−2.

The location of the variant protein was determined according to results from a number of different software programs and analyses, including analyses from SignalP and other specialized programs. The variant protein is believed to be located as follows with regard to the cell: secreted. The protein localization is believed to be secreted because both signal-peptide prediction programs predict that this protein has a signal peptide, and neither trans-membrane region prediction program predicts that this protein has a trans-membrane region.

Variant protein D56406_PEA_(—)1_P5 (SEQ ID NO: 162) also has the following non-silent SNPs (Single Nucleotide Polymorphisms) as listed in Table 6, (given according to their position(s) on the amino acid sequence, with the alternative amino acid(s) listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein D56406_PEA_(—)1_P5 (SEQ ID NO: 162) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 6 Amino acid mutations SNP position(s) on amino acid Alternative sequence amino acid(s) Previously known SNP? 28 M -> V No 42 S -> P No 82 V -> No 82 V -> A No

Variant protein D56406_PEA_(—)1_P5 (SEQ ID NO: 162) is encoded by the following transcript(s): D56406_PEA_(—)1_T6 (SEQ ID NO: 148), for which the sequence(s) is/are given at the end of the application. The coding portion of transcript D56406_PEA_(—)1_T6 (SEQ ID NO: 148) is shown in bold; this coding portion starts at position 106 and ends at position 609. The transcript also has the following SNPs as listed in Table 7 (given according to their position on the nucleotide sequence, with the alternative nucleic acid listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein D56406_PEA_(—)1_P5 (SEQ ID NO: 162) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 7 Nucleic acid SNPs SNP position on nucleotide Alternative sequence nucleic acid Previously known SNP? 94 G -> T No 95 A -> T No 759 T -> G Yes 806 G -> A Yes 1014 T -> G No 1178 T -> G No 103 A -> G Yes 187 A -> G No 229 T -> C No 333 T -> C No 350 T -> No 350 T -> C No 411 A -> T No 658 T -> No

Variant protein D56406_PEA_(—)1_P6 (SEQ ID NO: 163) according to the present invention has an amino acid sequence as given at the end of the application; it is encoded by transcript(s) D56406_PEA_(—)1_T7 (SEQ ID NO: 149). An alignment is given to the known protein (Neurotensin/neuromedin N precursor) at the end of the application. One or more alignments to one or more previously published protein sequences are given at the end of the application. A brief description of the relationship of the variant protein according to the present invention to each such aligned protein is as follows:

Comparison report between D56406_PEA_(—)1_P6 (SEQ ID NO: 163) and NEUT_HUMAN:

1. An isolated chimeric polypeptide encoding for D56406_PEA_(—)1_P6 (SEQ ID NO: 163), comprising a first amino acid sequence being at least 90% homologous to MMAGMKIQLVCMLLLAFSSWSLCSDSEEEMKALEADFLTNMHTSK corresponding to amino acids 1-45 of NEUT_HUMAN, which also corresponds to amino acids 1-45 of D56406_PEA_(—)1_P6 (SEQ ID NO: 163), and a second amino acid sequence being at least 90% homologous to LIQEDILDTGNDKNGKEEVIKRKIPYILKRQLYENKPRRPYILKRDSYYY corresponding to amino acids 121-170 of NEUT_HUMAN, which also corresponds to amino acids 46-95 of D56406_PEA_(—)1_P6 (SEQ ID NO: 163), wherein said first and second amino acid sequences are contiguous and in a sequential order.

2. An isolated chimeric polypeptide encoding for an edge portion of D56406_PEA_(—)1_P6 (SEQ ID NO: 163), comprising a polypeptide having a length “n”, wherein n is at least about 10 amino acids in length, optionally at least about 20 amino acids in length, preferably at least about 30 amino acids in length, more preferably at least about 40 amino acids in length and most preferably at least about 50 amino acids in length, wherein at least two amino acids comprise KL, having a structure as follows: a sequence starting from any of amino acid numbers 45-x to 45; and ending at any of amino acid numbers 46+((n−2)−x), in which x varies from 0 to n−2.

The location of the variant protein was determined according to results from a number of different software programs and analyses, including analyses from SignalP and other specialized programs. The variant protein is believed to be located as follows with regard to the cell: secreted. The protein localization is believed to be secreted because both signal-peptide prediction programs predict that this protein has a signal peptide, and neither trans-membrane region prediction program predicts that this protein has a trans-membrane region.

Variant protein D56406_PEA_(—)1_P6 (SEQ ID NO: 163) also has the following non-silent SNPs (Single Nucleotide Polymorphisms) as listed in Table 8, (given according to their position(s) on the amino acid sequence, with the alternative amino acid(s) listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein D56406_PEA_(—)1_P6 (SEQ ID NO: 163) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 8 Amino acid mutations SNP position(s) on amino acid Alternative sequence amino acid(s) Previously known SNP? 30 M -> V No 44 S -> P No

Variant protein D56406_PEA_(—)1_P6 (SEQ ID NO: 163) is encoded by the following transcript(s): D56406_PEA_(—)1_T7 (SEQ ID NO: 149), for which the sequence(s) is/are given at the end of the application. The coding portion of transcript D56406_PEA_(—)1_T7 (SEQ ID NO: 149) is shown in bold; this coding portion starts at position 106 and ends at position 390. The transcript also has the following SNPs as listed in Table 9 (given according to their position on the nucleotide sequence, with the alternative nucleic acid listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein D56406_PEA_(—)1_P6 (SEQ ID NO: 163) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 9 Nucleic acid SNPs SNP position on nucleotide Alternative sequence nucleic acid Previously known SNP? 94 G -> T No 95 A -> T No 103 A -> G Yes 193 A -> G No 235 T -> C No 439 T -> No 540 T -> G Yes 587 G -> A Yes 795 T -> G No 959 T -> G No

As noted above, cluster D56406 features 10 segment(s), which were listed in Table 2 above and for which the sequence(s) are given at the end of the application. These segment(s) are portions of nucleic acid sequence(s) which are described herein separately because they are of particular interest. A description of each segment according to the present invention is now provided.

Segment cluster D56406_PEA_(—)1_node_(—)0 (SEQ ID NO: 150) according to the present invention is supported by 48 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): D56406_PEA_(—)1_T3 (SEQ ID NO: 147), D56406_PEA_(—)1_T6 (SEQ ID NO: 148) and D56406_PEA_(—)1_T7 (SEQ ID NO: 149). Table 10 below describes the starting and ending position of this segment on each transcript.

TABLE 10 Segment location on transcripts Segment Segment Transcript starting ending name position position D56406_PEA_1_T3 1 178 (SEQ ID NO:147) D56406_PEA_1_T6 1 178 (SEQ ID NO:148) D56406_PEA_1_T7 1 178 (SEQ ID NO:149)

Microarray (chip) data is also available for this segment as follows. As described above with regard to the cluster itself, various oligonucleotides were tested for being differentially expressed in various disease conditions, particularly cancer. The following oligonucleotides were found to hit this segment (with regard to ovarian cancer), shown in Table 11.

TABLE 11 Oligonucleotides related to this segment Oligonucleotide Overexpressed Chip name in cancers reference D56406_0_5_0 ovarian OVA (SEQ ID NO:1015) carcinoma

Segment cluster D56406_PEA_(—)1_node_(—)13 (SEQ ID NO: 151) according to the present invention is supported by 43 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): D56406_PEA_(—)1_T3 (SEQ ID NO: 147), D56406_PEA_(—)1_T6 (SEQ ID NO: 148) and D56406_PEA_(—)1_T7 (SEQ ID NO: 149). Table 12 below describes the starting and ending position of this segment on each transcript.

TABLE 12 Segment location on transcripts Segment Segment Transcript starting ending name position position D56406_PEA_1_T3 559 902 (SEQ ID NO:147) D56406_PEA_1_T6 460 1239 (SEQ ID NO:148) D56406_PEA_1_T7 241 1020 (SEQ ID NO:149)

According to an optional embodiment of the present invention, short segments related to the above cluster are also provided. These segments are up to about 120 bp in length, and so are included in a separate description.

Segment cluster D56406_PEA_(—)1_node_(—)11 (SEQ ID NO: 152) according to the present invention is supported by 1 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): D56406_PEA_(—)1_T3 (SEQ ID NO: 147). Table 13 below describes the starting and ending position of this segment on each transcript.

TABLE 13 Segment location on transcripts Segment Segment Transcript starting ending name position position D56406_PEA_1_T3 466 558 (SEQ ID NO:147)

Segment cluster D56406_PEA_(—)1_node_(—)2 (SEQ ID NO: 153) according to the present invention can be found in the following transcript(s): D56406_PEA_(—)1_T3 (SEQ ID NO: 147) and D56406_PEA_(—)1_T7 (SEQ ID NO: 149). Table 14 below describes the starting and ending position of this segment on each transcript.

TABLE 14 Segment location on transcripts Segment Segment Transcript starting ending name position position D56406_PEA_1_T3 179 184 (SEQ ID NO:147) D56406_PEA_1_T7 179 184 (SEQ ID NO:149)

Segment cluster D56406_PEA_(—)1_node_(—)3 (SEQ ID NO: 154) according to the present invention is supported by 46 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): D56406_PEA_(—)1_T3 (SEQ ID NO: 147), D56406_PEA_(—)1_T6 (SEQ ID NO: 148) and D56406_PEA_(—)1_T7 (SEQ ID NO: 149). Table 15 below describes the starting and ending position of this segment on each transcript.

TABLE 15 Segment location on transcripts Segment Segment Transcript starting ending name position position D56406_PEA_1_T3 185 240 (SEQ ID NO:147) D56406_PEA_1_T6 179 234 (SEQ ID NO:148) D56406_PEA_1_T7 185 240 (SEQ ID NO:149)

Segment cluster D56406_PEA_(—)1_node_(—)5 (SEQ ID NO: 155) according to the present invention is supported by 48 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): D56406_PEA_(—)1_T3 (SEQ ID NO: 147) and D56406_PEA_(—)1_T6 (SEQ ID NO: 148). Table 16 below describes the starting and ending position of this segment on each transcript.

TABLE 16 Segment location on transcripts Segment Segment Transcript starting ending name position position D56406_PEA_1_T3 241 355 (SEQ ID NO:147) D56406_PEA_1_T6 235 349 (SEQ ID NO:148)

Segment cluster D56406_PEA_(—)1_node_(—)6 (SEQ ID NO: 156) according to the present invention is supported by 34 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): D56406_PEA_(—)1_T3 (SEQ ID NO: 147) and D56406_PEA_(—)1_T6 (SEQ ID NO: 148). Table 17 below describes the starting and ending position of this segment on each transcript.

TABLE 17 Segment location on transcripts Segment Segment Transcript starting ending name position position D56406_PEA_1_T3 356 389 (SEQ ID NO:147) D56406_PEA_1_T6 350 383 (SEQ ID NO:148)

Segment cluster D56406_PEA_(—)1_node_(—)7 (SEQ ID NO: 157) according to the present invention is supported by 32 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): D56406_PEA_(—)1_T3 (SEQ ID NO: 147) and D56406_PEA_(—)1_T6 (SEQ ID NO: 148). Table 18 below describes the starting and ending position of this segment on each transcript.

TABLE 18 Segment location on transcripts Segment Segment Transcript starting ending name position position D56406_PEA_1_T3 390 415 (SEQ ID NO:147) D56406_PEA_1_T6 384 409 (SEQ ID NO:148)

Segment cluster D56406_PEA_(—)1_node_(—)8 (SEQ ID NO: 158) according to the present invention can be found in the following transcript(s): D56406_PEA_(—)1_T3 (SEQ ID NO: 147) and D56406_PEA_(—)1_T6 (SEQ ID NO: 148). Table 19 below describes the starting and ending position of this segment on each transcript.

TABLE 19 Segment location on transcripts Segment Segment Transcript starting ending name position position D56406_PEA_1_T3 416 423 (SEQ ID NO:147) D56406_PEA_1_T6 410 417 (SEQ ID NO:148)

Segment cluster D56406_PEA_(—)1_node_(—)9 (SEQ ID NO: 159) according to the present invention is supported by 31 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): D56406_PEA_(—)1_T3 (SEQ ID NO: 147) and D56406_PEA_(—)1_T6 (SEQ ID NO: 148). Table 20 below describes the starting and ending position of this segment on each transcript.

TABLE 20 Segment location on transcripts Segment Segment Transcript starting ending name position position D56406_PEA_1_T3 424 465 (SEQ ID NO:147) D56406_PEA_1_T6 418 459 (SEQ ID NO:148) Variant protein alignment to the previously known protein: Sequence name: /tmp/jU49325aMA/8F0XuN7La5:NEUT_HUMAN Sequence documentation:

Alignment of: D56406_PEA_(—)1_P2 (SEQ ID NO: 161) x NEUT_HUMAN ..

Alignment segment 1/1:

Quality: 1591.00 Escore: 0 Matching length: 170 Total length: 201 Matching Percent Similarity: 100.00 Matching Percent Identity: 100.00 Total Percent Similarity: 84.58 Total Percent Identity: 84.58 Gaps: 1

Alignment:

Sequence name: /tmp/wWui8 Kd4y9/zbf31hRwnR:NEUT_HUMAN Sequence documentation:

Alignment of: D56406_PEA_(—)1_P5 (SEQ ID NO: 162) x NEUT_HUMAN ..

Alignment segment 1/1:

Quality: 1572.00 Escore: 0 Matching length: 168 Total length: 170 Matching Percent Similarity: 100.00 Matching Percent Identity: 100.00 Total Percent Similarity: 98.82 Total Percent Identity: 98.82 Gaps: 1

Alignment:

Sequence name: /tmp/f5d07fF5D7/E4N5xjUIAN:NEUT_HUMAN Sequence documentation:

Alignment of: D56406_PEA_(—)1_P6 (SEQ ID NO: 163) x NEUT_HUMAN ..

Alignment segment 1/1:

Quality: 844.00 Escore: 0 Matching length: 95 Total length: 170 Matching Percent Similarity: 100.00 Matching Percent Identity: 100.00 Total Percent Similarity: 55.88 Total Percent Identity: 55.88 Gaps: 1

Alignment:

Description for Cluster H53393

Cluster H53393 features 4 transcript(s) and 16 segment(s) of interest, the names for which are given in Tables 1 and 2, respectively, the sequences themselves are given at the end of the application. The selected protein variants are given in table 3.

TABLE 1 Transcripts of interest Transcript Name SEQ ID NO: H53393_PEA_1_T10 164 H53393_PEA_1_T11 165 H53393_PEA_1_T3 166 H53393_PEA_1_T9 167

TABLE 2 Segments of interest Segment Name SEQ ID NO: H53393_PEA_1_node_0 168 H53393_PEA_1_node_10 169 H53393_PEA_1_node_12 170 H53393_PEA_1_node_13 171 H53393_PEA_1_node_15 172 H53393_PEA_1_node_17 173 H53393_PEA_1_node_19 174 H53393_PEA_1_node_23 175 H53393_PEA_1_node_24 176 H53393_PEA_1_node_25 177 H53393_PEA_1_node_29 178 H53393_PEA_1_node_4 179 H53393_PEA_1_node_6 180 H53393_PEA_1_node_8 181 H53393_PEA_1_node_21 182 H53393_PEA_1_node_22 183

TABLE 3 Proteins of interest Protein Name SEQ ID NO: H53393_PEA_1_P2 185 H53393_PEA_1_P3 186 H53393_PEA_1_P6 187

These sequences are variants of the known protein Cadherin-6 precursor (SwissProt accession identifier CAD6_HUMAN; known also according to the synonyms Kidney-cadherin; K-cadherin), SEQ ID NO: 184, referred to herein as the previously known protein.

Protein Cadherin-6 precursor is known or believed to have the following function(s): Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. The sequence for protein Cadherin-6 precursor is given at the end of the application, as “Cadherin-6 precursor amino acid sequence”. Known polymorphisms for this sequence are as shown in Table 4.

TABLE 4 Amino acid mutations for Known Protein SNP position(s) on amino acid sequence Comment 421 V -> I 425 T -> I

Protein Cadherin-6 precursor localization is believed to be Type I membrane protein.

The following GO Annotation(s) apply to the previously known protein. The following annotation(s) were found: cell adhesion; homophilic cell adhesion, which are annotation(s) related to Biological Process; calcium binding; protein binding, which are annotation(s) related to Molecular Function; and integral membrane protein, which are annotation(s) related to Cellular Component.

The GO assignment relies on information from one or more of the SwissProt/TremBl Protein knowledgebase, available from <http://www.expasy.ch/sprot/>; or Locuslink, available from <http://www.ncbi.nlm.nih.gov/projects/LocusLink/>.

Cluster H53393 can be used as a diagnostic marker according to overexpression of transcripts of this cluster in cancer. Expression of such transcripts in normal tissues is also given according to the previously described methods. The term “number” in the left hand column of the table and the numbers on the y-axis of FIG. 20 refer to weighted expression of ESTs in each category, as “parts per million” (ratio of the expression of ESTs for a particular cluster to the expression of all ESTs in that category, according to parts per million).

Overall, the following results were obtained as shown with regard to the histograms in FIG. 20 and Table 5. This cluster is overexpressed (at least at a minimum level) in the following pathological conditions: epithelial malignant tumors, a mixture of malignant tumors from different tissues and ovarian carcinoma.

TABLE 5 Normal tissue distribution Name of Tissue Number epithelial 2 general 5 kidney 15 lung 6 muscle 5 ovary 0 uterus 0

TABLE 6 P values and ratios for expression in cancerous tissue Name of Tissue P1 P2 SP1 R3 SP2 R4 epithelial 1.4e−01 1.1e−01 1.8e−04 6.3 2.5e−05 5.9 general 2.0e−01 8.6e−02 1.1e−04 3.1 1.3e−06 3.2 kidney 5.5e−01 4.4e−01 3.4e−01 1.7 8.2e−02 2.3 lung 9.5e−01 8.5e−01 1 0.6 6.2e−01 1.1 muscle 9.2e−01 4.8e−01 1 0.8 3.9e−01 2.0 ovary 7.1e−02 3.0e−02 1.5e−02 5.2 2.9e−03 5.9 uterus 8.2e−02 1.4e−01 1.9e−01 3.0 3.3e−01 2.2

As noted above, cluster H53393 features 4 transcript(s), which were listed in Table 1 above. These transcript(s) encode for protein(s) which are variant(s) of protein Cadherin-6 precursor. A description of each variant protein according to the present invention is now provided.

Variant protein H53393_PEA_(—)1_P2 (SEQ ID NO: 185) according to the present invention has an amino acid sequence as given at the end of the application; it is encoded by transcript(s) H53393_PEA_(—)1_T10 (SEQ ID NO: 164). An alignment is given to the known protein (Cadherin-6 precursor) at the end of the application. One or more alignments to one or more previously published protein sequences are given at the end of the application. A brief description of the relationship of the variant protein according to the present invention to each such aligned protein is as follows:

Comparison report between H53393_PEA_(—)1_P2 (SEQ ID NO: 185) and CAD6_HUMAN:

1. An isolated chimeric polypeptide encoding for H53393_PEA_(—)1_P2 (SEQ ID NO: 185), comprising a first amino acid sequence being at least 90% homologous to MRTYRYFLLLFWVGQPYPTLSTPLSKRTSGFPAKKRALELSGNSKNELNRSKRSWMWN QFFLLEEYTGSDYQYVGKLHSDQDRGDGSLKYILSGDGAGDLFIINENTGDIQATKRLD REEKPVYILRAQAINRRTGRPVEPESEFIIKIHDINDNEPIFTKEVYTATVPEMSDVGTFVV QVTATDADDPTYGNSAKVVYSILQGQPYFSVESETGIIKTALLNMDRENREQYQVVIQA KDMGGQMGGLSGTTTVNITLTDVNDNPPRFPQSTYQFKTPESSPPGTPIGRIKASDADV GENAEIEYSITDGEGLDMFDVITDQETQEGIITVKKLLDFEKKKVYTLKVEASNPYVEPR FLYLGPFKDSATVRIVVEDVDEPPVFSKLAYILQIREDAQINTTIGSVTAQDPDAARNPV KYSVDRHTDMDRIFNIDSGNGSIFTSKLLDRETLLWHNITVIATEINNPKQSSRVPLYIKV LDVNDNAPEFAEFYETFVCEKAKADQLIQTLHAVDKDDPYSGHQFSFSLAPEAASGSNF TIQDNK corresponding to amino acids 1-543 of CAD6_HUMAN, which also corresponds to amino acids 1-543 of H53393_PEA_(—)1_P2 (SEQ ID NO: 185), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence GK corresponding to amino acids 544-545 of H53393_PEA_(—)1_P2 (SEQ ID NO: 185), wherein said first and second amino acid sequences are contiguous and in a sequential order.

The location of the variant protein was determined according to results from a number of different software programs and analyses, including analyses from SignalP and other specialized programs. The variant protein is believed to be located as follows with regard to the cell: secreted. The protein localization is believed to be secreted because both signal-peptide prediction programs predict that this protein has a signal peptide, and neither trans-membrane region prediction program predicts that this protein has a trans-membrane region.

Variant protein H53393_PEA_(—)1_P2 (SEQ ID NO: 185) is encoded by the following transcript(s): H53393_PEA_(—)1_T10 (SEQ ID NO: 164), for which the sequence(s) is/are given at the end of the application. The coding portion of transcript H53393_PEA_(—)1_T10 (SEQ ID NO: 164) is shown in bold; this coding portion starts at position 327 and ends at position 1961. The transcript also has the following SNPs as listed in Table 7 (given according to their position on the nucleotide sequence, with the alternative nucleic acid listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein H53393_PEA_(—)1_P2 (SEQ ID NO: 185) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 7 Nucleic acid SNPs SNP position on nucleotide Previously sequence Alternative nucleic acid known SNP? 1208 C -> T Yes 1407 T -> C Yes 1851 T -> C Yes 1886 G -> A Yes 2309 C -> T Yes 2736 T -> C Yes 2762 G -> T Yes

Variant protein H53393_PEA_(—)1_P3 (SEQ ID NO: 186) according to the present invention has an amino acid sequence as given at the end of the application; it is encoded by transcript(s) H53393_PEA_(—)1_T11 (SEQ ID NO: 165) and H53393_PEA_(—)1_T3 (SEQ ID NO: 166). An alignment is given to the known protein (Cadherin-6 precursor) at the end of the application. One or more alignments to one or more previously published protein sequences are given at the end of the application. A brief description of the relationship of the variant protein according to the present invention to each such aligned protein is as follows:

Comparison report between H53393_PEA_(—)1_P3 (SEQ ID NO: 186) and CAD6_HUMAN:

1. An isolated chimeric polypeptide encoding for H53393_PEA_(—)1_P3 (SEQ ID NO: 186), comprising a first amino acid sequence being at least 90% homologous to MRTYRYFLLLFWVGQPYPTLSTPLSKRTSGFPAKKRALELSGNSKNELNRSKRSWMWN QFFLLEEYTGSDYQYVGKLHSDQDRGDGSLKYILSGDGAGDLFIINENTGDIQATKRLD REEKPVYILRAQAINRRTGRPVEPESEFIIKIHDINDNEPIFTKEVYTATVPEMSDVGTFVV QVTATDADDPTYGNSAKVVYSILQGQPYFSVESETGIIKTALLNMDRENREQYQVVIQA KDMGGQMGGLSGTTTVNITLTDVNDNPPRFPQSTYQFKTPESSPPGTPIGRIKASDADV GENAEIEYSITDGEGLDMFDVITDQETQEGIITVKKLLDFEKKKVYTLKVEASNPYVEPR FLYLGPFKDSATVRIVVEDVDEPPVFSKLAYILQIREDAQINTTIGSVTAQDPDAARNPV KYSVDRHTDMDRIFNIDSGNGSIFTSKLLDRETLLWHNITVIATEINNPKQSSRVPLYIKV LDVNDNAPEFAEFYETFVCEKAKADQ corresponding to amino acids 1-504 of CAD6_HUMAN, which also corresponds to amino acids 1-504 of H53393_PEA_(—)1_P3 (SEQ ID NO: 186), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence RFGFSLS (SEQ ID NO: 1133) corresponding to amino acids 505-511 of H53393_PEA_(—)1_P3 (SEQ ID NO: 186), wherein said first and second amino acid sequences are contiguous and in a sequential order.

2. An isolated polypeptide encoding for a tail of H53393_PEA_(—)1_P3 (SEQ ID NO: 186), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence RFGFSLS (SEQ ID NO: 1133) in H53393_PEA_(—)1_P3 (SEQ ID NO: 186).

The location of the variant protein was determined according to results from a number of different software programs and analyses, including analyses from SignalP and other specialized programs. The variant protein is believed to be located as follows with regard to the cell: secreted. The protein localization is believed to be secreted because both signal-peptide prediction programs predict that this protein has a signal peptide, and neither trans-membrane region prediction program predicts that this protein has a trans-membrane region.

Variant protein H53393_PEA_(—)1_P3 (SEQ ID NO: 186) is encoded by the following transcript(s): H53393_PEA_(—)1_T11 (SEQ ID NO: 165) and H53393_PEA_(—)1_T3 (SEQ ID NO: 166), for which the sequence(s) is/are given at the end of the application.

The coding portion of transcript H53393_PEA_(—)1_T11 (SEQ ID NO: 165) is shown in bold; this coding portion starts at position 327 and ends at position 1859. The transcript also has the following SNPs as listed in Table 8 (given according to their position on the nucleotide sequence, with the alternative nucleic acid listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein H53393_PEA_(—)1_P3 (SEQ ID NO: 186) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 8 Nucleic acid SNPs SNP position on nucleotide Previously sequence Alternative nucleic acid known SNP? 1208 C -> T Yes 1407 T -> C Yes 1871 T -> C Yes 1906 G -> A Yes 2329 C -> T Yes 2756 T -> C Yes 2782 G -> T Yes

The coding portion of transcript H53393_PEA_(—)1_T3 (SEQ ID NO: 166) is shown in bold; this coding portion starts at position 327 and ends at position 1859. The transcript also has the following SNPs as listed in Table 9 (given according to their position on the nucleotide sequence, with the alternative nucleic acid listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein H53393_PEA_(—)1_P3 (SEQ ID NO: 186) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 9 Nucleic acid SNPs SNP position on nucleotide Previously sequence Alternative nucleic acid known SNP? 1208 C -> T Yes 1407 T -> C Yes 1871 T -> C Yes 1906 G -> A Yes 2149 C -> T Yes 3425 T -> No 3492 C -> G Yes

Variant protein H53393_PEA_(—)1_P6 (SEQ ID NO: 187) according to the present invention has an amino acid sequence as given at the end of the application; it is encoded by transcript(s) H53393_PEA_(—)1_T9 (SEQ ID NO: 167). An alignment is given to the known protein (Cadherin-6 precursor) at the end of the application. One or more alignments to one or more previously published protein sequences are given at the end of the application. A brief description of the relationship of the variant protein according to the present invention to each such aligned protein is as follows:

Comparison report between H53393_PEA_(—)1_P6 (SEQ ID NO: 187) and CAD6_HUMAN:

1. An isolated chimeric polypeptide encoding for H53393_PEA_(—)1_P6 (SEQ ID NO: 187), comprising a first amino acid sequence being at least 90% homologous to MRTYRYFLLLFWVGQPYPTLSTPLSKRTSGFPAKKRALELSGNSKNELNRSKRSWMWN QFFLLEEYTGSDYQYVGKLHSDQDRGDGSLKYILSGDGAGDLFIINENTGDIQATKRLD REEKPVYILRAQAINRRTGRPVEPESEFIIKIHDINDNEPIFTKEVYTATVPEMSDVGTFVV QVTATDADDPTYGNSAKVVYSILQGQPYFSVESETGIIKTALLNMDRENREQYQVVIQA KDMGGQMGGLSGTTTVNITLTDVNDNPPRFPQSTYQFKTPESSPPGTPIGRIKASDADV GENAEIEYSITDGEGLDMFDVITDQETQEGIITVKK corresponding to amino acids 1-333 of CAD6_HUMAN, which also corresponds to amino acids 1-333 of H53393_PEA_(—)1_P6 (SEQ ID NO: 187), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence VMPLLKHHTE (SEQ ID NO: 1134) corresponding to amino acids 334-343 of H53393_PEA_(—)1_P6 (SEQ ID NO: 187), wherein said first and second amino acid sequences are contiguous and in a sequential order.

2. An isolated polypeptide encoding for a tail of H53393_PEA_(—)1_P6 (SEQ ID NO: 187), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence VMPLLKHHTE (SEQ ID NO: 1134) in H53393_PEA_(—)1_P6 (SEQ ID NO: 187).

The location of the variant protein was determined according to results from a number of different software programs and analyses, including analyses from SignalP and other specialized programs. The variant protein is believed to be located as follows with regard to the cell: secreted. The protein localization is believed to be secreted because both signal-peptide prediction programs predict that this protein has a signal peptide, and neither trans-membrane region prediction program predicts that this protein has a trans-membrane region.

Variant protein H53393_PEA_(—)1_P6 (SEQ ID NO: 187) is encoded by the following transcript(s): H53393_PEA_(—)1_T9 (SEQ ID NO: 167), for which the sequence(s) is/are given at the end of the application. The coding portion of transcript H53393_PEA_(—)1_T9 (SEQ ID NO: 167) is shown in bold; this coding portion starts at position 327 and ends at position 1355. The transcript also has the following SNPs as listed in Table 10 (given according to their position on the nucleotide sequence, with the alternative nucleic acid listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein H53393_PEA_(—)1_P6 (SEQ ID NO: 187) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 10 Nucleic acid SNPs SNP position on nucleotide Previously sequence Alternative nucleic acid known SNP? 1208 C -> T Yes

As noted above, cluster H53393 features 16 segment(s), which were listed in Table 2 above and for which the sequence(s) are given at the end of the application. These segment(s) are portions of nucleic acid sequence(s) which are described herein separately because they are of particular interest. A description of each segment according to the present invention is now provided.

Segment cluster H53393_PEA_(—)1_node_(—)0 (SEQ ID NO: 168) according to the present invention is supported by 10 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): H53393_PEA_(—)1_T10 (SEQ ID NO: 164), H53393_PEA_(—)1_T11 (SEQ ID NO: 165), H53393_PEA_(—)1_T3 (SEQ ID NO: 166) and H53393_PEA_(—)1_T9 (SEQ ID NO: 167). Table 11 below describes the starting and ending position of this segment on each transcript.

TABLE 11 Segment location on transcripts Segment Segment Transcript starting ending name position position H53393_PEA_1_T10 1 198 (SEQ ID NO:164) H53393_PEA_1_T11 1 198 (SEQ ID NO:165) H53393_PEA_1_T3 1 198 (SEQ ID NO:166) H53393_PEA_1_T9 1 198 (SEQ ID NO:167)

Segment cluster H53393_PEA_(—)1_node_(—)10 (SEQ ID NO: 169) according to the present invention is supported by 10 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): H53393_PEA_(—)1_T10 (SEQ ID NO: 164), H53393_PEA_(—)1_T11 (SEQ ID NO: 165), H53393_PEA_(—)1_T3 (SEQ ID NO: 166) and H53393_PEA_(—)1_T9 (SEQ ID NO: 167). Table 12 below describes the starting and ending position of this segment on each transcript.

TABLE 12 Segment location on transcripts Segment Segment Transcript starting ending name position position H53393_PEA_1_T10 970 1137 (SEQ ID NO:164) H53393_PEA_1_T11 970 1137 (SEQ ID NO:165) H53393_PEA_1_T3 970 1137 (SEQ ID NO:166) H53393_PEA_1_T9 970 1137 (SEQ ID NO:167)

Segment cluster H53393_PEA_(—)1_node_(—)12 (SEQ ID NO: 170) according to the present invention is supported by 7 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): H53393_PEA_(—)1_T10 (SEQ ID NO: 164), H53393_PEA_(—)1_T11 (SEQ ID NO: 165), H53393_PEA_(—)1_T3 (SEQ ID NO: 166) and H53393_PEA_(—)1_T9 (SEQ ID NO: 167). Table 13 below describes the starting and ending position of this segment on each transcript.

TABLE 13 Segment location on transcripts Segment Segment Transcript starting ending name position position H53393_PEA_1_T10 1138 1325 (SEQ ID NO:164) H53393_PEA_1_T11 1138 1325 (SEQ ID NO:165) H53393_PEA_1_T3 1138 1325 (SEQ ID NO:166) H53393_PEA_1_T9 1138 1325 (SEQ ID NO:167)

Segment cluster H53393_PEA_(—)1_node_(—)13 (SEQ ID NO: 171) according to the present invention is supported by 2 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): H53393_PEA_(—)1_T9 (SEQ ID NO: 167). Table 14 below describes the starting and ending position of this segment on each transcript.

TABLE 14 Segment location on transcripts Segment Segment Transcript starting ending name position position H53393_PEA_1_T9 1326 1625 (SEQ ID NO:167)

Segment cluster H53393_PEA_(—)1_node_(—)15 (SEQ ID NO: 172) according to the present invention is supported by 11 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): H53393_PEA_(—)1_T10 (SEQ ID NO: 164), H53393_PEA_(—)1_T11 (SEQ ID NO: 165) and H53393_PEA_(—)1_T3 (SEQ ID NO: 166). Table 15 below describes the starting and ending position of this segment on each transcript.

TABLE 15 Segment location on transcripts Segment Segment Transcript starting ending name position position H53393_PEA_1_T10 1326 1579 (SEQ ID NO:164) H53393_PEA_1_T11 1326 1579 (SEQ ID NO:165) H53393_PEA_1_T3 1326 1579 (SEQ ID NO:166)

Segment cluster H53393_PEA_(—)1_node_(—)17 (SEQ ID NO: 173) according to the present invention is supported by 13 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): H53393_PEA_(—)1_T10 (SEQ ID NO: 164), H53393_PEA_(—)1_T11 (SEQ ID NO: 165) and H53393_PEA_(—)1_T3 (SEQ ID NO: 166). Table 16 below describes the starting and ending position of this segment on each transcript.

TABLE 16 Segment location on transcripts Segment Segment Transcript name starting position ending position H53393_PEA_1_T10 1580 1716 (SEQ ID NO: 164) H53393_PEA_1_T11 1580 1716 (SEQ ID NO: 165) H53393_PEA 1_T3 1580 1716 (SEQ ID NO: 166)

Segment cluster H53393_PEA_(—)1_node_(—)19 (SEQ ID NO: 174) according to the present invention is supported by 16 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): H53393_PEA_(—)1_T10 (SEQ ID NO: 164), H53393_PEA_(—)1_T11 (SEQ ID NO: 165) and H53393_PEA_(—)1_T3 (SEQ ID NO: 166). Table 17 below describes the starting and ending position of this segment on each transcript.

TABLE 17 Segment location on transcripts Segment Segment Transcript name starting position ending position H53393_PEA_1_T10 1717 1838 (SEQ ID NO: 164) H53393_PEA_1_T11 1717 1838 (SEQ ID NO: 165) H53393_PEA_1_T3 1717 1838 (SEQ ID NO: 166)

Segment cluster H53393_PEA_(—)1_node_(—)23 (SEQ ID NO: 175) according to the present invention is supported by 1 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): H53393_PEA_(—)1_T10 (SEQ ID NO: 164) and H53393_PEA_(—)1_T11 (SEQ ID NO: 165). Table 18 below describes the starting and ending position of this segment on each transcript.

TABLE 18 Segment location on transcripts Segment Segment Transcript name starting position ending position H53393_PEA_1_T10 1957 2136 (SEQ ID NO: 164) H53393_PEA_1_T11 1977 2156 (SEQ ID NO: 165)

Segment cluster H53393_PEA_(—)1_node_(—)24 (SEQ ID NO: 176) according to the present invention is supported by 19 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): H53393_PEA_(—)1_T10 (SEQ ID NO: 164), H53393_PEA_(—)1_T11 (SEQ ID NO: 165) and H53393_PEA_(—)1_T3 (SEQ ID NO: 166). Table 19 below describes the starting and ending position of this segment on each transcript.

TABLE 19 Segment location on transcripts Segment Segment Transcript name starting position ending position H53393_PEA_1_T10 2137 2388 (SEQ ID NO: 164) H53393_PEA_1_T11 2157 2408 (SEQ ID NO: 165) H53393_PEA_1_T3 1977 2228 (SEQ ID NO: 166)

Segment cluster H53393_PEA_(—)1_node_(—)25 (SEQ ID NO: 177) according to the present invention is supported by 6 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): H53393_PEA_(—)1_T10 (SEQ ID NO: 164) and H53393_PEA_(—)1_T11 (SEQ ID NO: 165). Table 20 below describes the starting and ending position of this segment on each transcript.

TABLE 20 Segment location on transcripts Segment Segment Transcript name starting position ending position H53393_PEA_1_T10 2389 2873 (SEQ ID NO: 164) H53393_PEA_1_T11 2409 2893 (SEQ ID NO: 165)

Segment cluster H53393_PEA_(—)1_node_(—)29 (SEQ ID NO: 178) according to the present invention is supported by 41 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): H53393_PEA_(—)1_T3 (SEQ ID NO: 166). Table 21 below describes the starting and ending position of this segment on each transcript.

TABLE 21 Segment location on transcripts Segment Segment Transcript name starting position ending position H53393_PEA_1_T3 2229 3998 (SEQ ID NO: 166)

Segment cluster H53393_PEA_(—)1_node_(—)4 (SEQ ID NO: 179) according to the present invention is supported by 12 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): H53393_PEA_(—)1_T10 (SEQ ID NO: 164), H53393_PEA_(—)1_T11 (SEQ ID NO: 165), H53393_PEA_(—)1_T3 (SEQ ID NO: 166) and H53393_PEA_(—)1_T9 (SEQ ID NO: 167). Table 22 below describes the starting and ending position of this segment on each transcript.

TABLE 22 Segment location on transcripts Segment Segment Transcript name starting position ending position H53393_PEA_1_T10 199 554 (SEQ ID NO: 164) H53393_PEA_1_T11 199 554 (SEQ ID NO: 165) H53393_PEA_1_T3 199 554 (SEQ ID NO: 166) H53393_PEA_1_T9 199 554 (SEQ ID NO: 167)

Segment cluster H53393_PEA_(—)1_node_(—)6 (SEQ ID NO: 180) according to the present invention is supported by 14 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): H53393_PEA_(—)1_T10 (SEQ ID NO: 164), H53393_PEA_(—)1_T11 (SEQ ID NO: 165), H53393_PEA_(—)1_T3 (SEQ ID NO: 166) and H53393_PEA_(—)1_T9 (SEQ ID NO: 167). Table 23 below describes the starting and ending position of this segment on each transcript.

TABLE 23 Segment location on transcripts Segment Segment Transcript name starting position ending position H53393_PEA_1_T10 555 849 (SEQ ID NO: 164) H53393_PEA_1_T11 555 849 (SEQ ID NO: 165) H53393_PEA_1_T3 555 849 (SEQ ID NO: 166) H53393_PEA_1_T9 555 849 (SEQ ID NO: 167)

Segment cluster H53393_PEA_(—)1_node_(—)8 (SEQ ID NO: 181) according to the present invention is supported by 12 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): H53393_PEA_(—)1_T10 (SEQ ID NO: 164), H53393_PEA_(—)1_T11 (SEQ ID NO: 165), H53393_PEA_(—)1_T3 (SEQ ID NO: 166) and H53393_PEA_(—)1_T9 (SEQ ID NO: 167). Table 24 below describes the starting and ending position of this segment on each transcript.

TABLE 24 Segment location on transcripts Segment Segment Transcript name starting position ending position H53393_PEA_1_T10 850 969 (SEQ ID NO: 164) H53393_PEA_1_T11 850 969 (SEQ ID NO: 165) H53393_PEA_1_T3 850 969 (SEQ ID NO: 166) H53393_PEA_1_T9 850 969 (SEQ ID NO: 167)

According to an optional embodiment of the present invention, short segments related to the above cluster are also provided. These segments are up to about 120 bp in length, and so are included in a separate description.

Segment cluster H53393_PEA_(—)1_node_(—)21 (SEQ ID NO: 182) according to the present invention can be found in the following transcript(s): H53393_PEA_(—)1_T11 (SEQ ID NO: 165) and H53393_PEA_(—)1_T3 (SEQ ID NO: 166). Table 25 below describes the starting and ending position of this segment on each transcript.

TABLE 25 Segment location on transcripts Segment Segment Transcript name starting position ending position H53393_PEA_1_T11 1839 1858 (SEQ ID NO: 165) H53393_PEA_1_T3 1839 1858 (SEQ ID NO: 166)

Segment cluster H53393_PEA_(—)1_node_(—)22 (SEQ ID NO: 183) according to the present invention is supported by 16 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): H53393_PEA_(—)1_T10 (SEQ ID NO: 164), H53393_PEA_(—)1_T11 (SEQ ID NO: 165) and H53393_PEA_(—)1_T3 (SEQ ID NO: 166). Table 26 below describes the starting and ending position of this segment on each transcript.

TABLE 26 Segment location on transcripts Segment Segment Transcript name starting position ending position H53393_PEA_1_T10 1839 1956 (SEQ ID NO: 164) H53393_PEA_1_T11 1859 1976 (SEQ ID NO: 165) H53393_PEA_1_T3 1859 1976 (SEQ ID NO: 166) Variant protein alignment to the previously known protein: Sequence name: /tmp/oAlc9u2qp7/1HgSZJi6aI:CAD6_HUMAN Sequence documentation:

Alignment of: H53393_PEA_(—)1_P2 (SEQ ID NO: 185) x CAD6_HUMAN ..

Alignment segment 1/1:

Quality: 5281.00 Escore: 0 Matching length: 543 Total length: 543 Matching Percent Similarity: 100.00 Matching Percent Identity: 100.00 Total Percent Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0

Alignment:

Sequence name: /tmp/I80QylyXbk/TP0IdL1tx5:CAD6_HUMAN Sequence documentation:

Alignment of: H53393_PEA_(—)1_P3 (SEQ ID NO: 186) x CAD6_HUMAN ..

Alignment segment 1/1:

Quality: 4900.00 Escore: 0 Matching length: 504 Total length: 504 Matching Percent Similarity: 100.00 Matching Percent Identity: 100.00 Total Percent Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0

Alignment:

Sequence name: /tmp/NtvjwylOCi/c5Li3O91on:CAD6_HUMAN Sequence documentation:

Alignment of: H53393_PEA_(—)1_P6 (SEQ ID NO: 187) x CAD6_HUMAN ..

Alignment segment 1/1:

Quality: 3247.00 Escore: 0 Matching length: 335 Total length: 335 Matching Percent Similarity: 100.00 Matching Percent Identity: 99.40 Total Percent Similarity: 100.00 Total Percent Identity: 99.40 Gaps: 0

Alignment:

Expression of CAD6_HUMAN Cadherin-6 [Precursor]; Kidney-Cadherin; K-Cadherin H53393 Transcripts which are Detectable by Amplicon as Depicted in Sequence Name H53393 seg13 (SEQ ID NO:981) in Normal and Cancerous Ovary Tissues

Expression of CAD6_HUMAN Cadherin-6 [Precursor]; Kidney-cadherin; K-cadherin transcripts detectable by or according to seg13, H53393 seg13 (SEQ ID NO:981) amplicon(s) and H53393 seg13F (SEQ ID NO:979) and H53393 seg13R (SEQ ID NO:980) primers was measured by real time PCR. In this specific example, the real-time PCR reaction efficiency was assumed to be 2 and was not calculated by a standard curve reaction (as detailed above in the section of “Real-Time RT-PCR analysis”). In parallel the expression of four housekeeping genes —PBGD (GenBank Accession No. BC019323, (SEQ ID NO:1036); amplicon—PBGD-amplicon, (SEQ ID NO:1039)), HPRT1 (GenBank Accession No. NM_(—)000194, (SEQ ID NO: 1040); amplicon—HPRT1-amplicon, (SEQ ID NO: 1043)), SDHA (GenBank Accession No. NM_(—)004168, (SEQ ID NO: 1032); amplicon—SDHA-amplicon, (SEQ ID NO: 1035)), and GAPDH (GenBank Accession No. BC026907, (SEQ ID NO: 1044); GAPDH amplicon, (SEQ ID NO:1047)) was measured similarly. For each RT sample, the expression of the above amplicon was normalized to the geometric mean of the quantities of the housekeeping genes. The normalized quantity of each RT sample was then divided by the median of the quantities of the normal post-mortem (PM) samples (Sample Nos. 45-48, 71, Table 1, “Tissue samples in testing panel”, above), to obtain a value of fold up-regulation for each sample relative to median of the normal PM samples.

FIG. 21 is a histogram showing over expression of the above-indicated CAD6_HUMAN Cadherin-6 [Precursor] transcripts in cancerous ovary samples relative to the normal samples.

As is evident from FIG. 21, the expression of CAD6_HUMAN Cadherin-6 [Precursor] transcripts detectable by the above amplicon(s) in cancer samples was significantly higher than in the non-cancerous samples (Sample Nos. 45-48, 71 Table 1, “Tissue samples in testing panel”). Notably an over-expression of at least 5 fold was found in 19 out of 43 adenocarcinoma samples.

Statistical analysis was applied to verify the significance of these results, as described below. The P value for the difference in the expression levels of CAD6_HUMAN Cadherin-6 [Precursor] transcripts detectable by the above amplicon(s) in ovary cancer samples versus the normal tissue samples was determined by T test as 5.5E-03.

Threshold of 5 fold overexpression was found to differentiate between cancer and normal samples with P value of 6.94E-02 as checked by exact fisher test. The above values demonstrate statistical significance of the results.

Primer pairs are also optionally and preferably encompassed within the present invention; for example, for the above experiment, the following primer pair was used as a non-limiting illustrative example only of a suitable primer pair: H53393 seg13F (SEQ ID NO:979) forward primer; and H53393 seg13R (SEQ ID NO:980) reverse primer.

The present invention also preferably encompasses any amplicon obtained through the use of any suitable primer pair; for example, for the above experiment, the following amplicon was obtained as a non-limiting illustrative example only of a suitable amplicon: H53393 seg13. H53393 seg13 Forward primer (SEQ ID NO:979): AATGCCGCTTCTTAAACACCA H53393 seg13 Reverse primer (SEQ ID NO:980): AGAACTGGCATTTTTCTGAAAATAATAA H53393 seg13 Amplicon (SEQ ID NO:981): AATGCCGCTTCTTAAACACCATACAGAGTGAACCCATTTACTTTTCTCCAGTTCCTA AGTTACCAGGGGCAATTATATCTCACATAAACATTCCTTTAGATTTTTATTTTACTTA TTATTTTCAGAAAAATGCCAGTTCT

Expression of CAD6_HUMAN Cadherin-6 [Precursor] H53393 transcripts which are detectable by amplicon as depicted in sequence name H53393 junc21-22 (SEQ ID NO:984) in normal and cancerous ovary tissues

Expression of CAD6_HUMAN Cadherin-6 [Precursor] transcripts detectable by or according to junc21-22, H53393 junc21-22 (SEQ ID NO:984) amplicon(s) and H53393 junc21-22F (SEQ ID NO:982) and H53393 junc21-22R (SEQ ID NO:983) primers was measured by real time PCR. In this specific example, the real-time PCR reaction efficiency was assumed to be 2 and was not calculated by a standard curve reaction (as detailed above in the section of “Real-Time RT-PCR analysis”). In parallel the expression of four housekeeping genes —PBGD (GenBank Accession No. BC019323, (SEQ ID NO:1036); amplicon—PBGD-amplicon, (SEQ ID NO: 1039)), HPRT1 (GenBank Accession No. NM_(—)000194, (SEQ ID NO: 1040); amplicon—HPRT1-amplicon, (SEQ ID NO:1043)), SDHA (GenBank Accession No. NM_(—)004168, (SEQ ID NO: 1032); amplicon—SDHA-amplicon, (SEQ ID NO: 1035)), and GAPDH (GenBank Accession No. BC026907, (SEQ ID NO:1044); GAPDH amplicon, (SEQ ID NO:1047)) was measured similarly. For each RT sample, the expression of the above amplicon was normalized to the geometric mean of the quantities of the housekeeping genes. The normalized quantity of each RT sample was then divided by the median of the quantities of the normal post-mortem (PM) samples (Sample Nos. 45-48, 71 Table 1, “Tissue samples in testing panel”, above), to obtain a value of fold up-regulation for each sample relative to median of the normal PM samples.

FIG. 22 is a histogram showing over expression of the above-indicated CAD6_HUMAN Cadherin-6 [Precursor] transcripts in cancerous ovary samples relative to the normal samples. As is evident from FIG. 22, the expression of CAD6_HUMAN Cadherin-6 [Precursor] transcripts detectable by the above amplicon(s) in cancer samples was higher than in the non-cancerous samples (Sample Nos. 45-48, 71 Table 1, “Tissue samples in testing panel”). Notably an over-expression of at least 5 fold was found in 23 out of 43 adenocarcinoma samples.

Primer pairs are also optionally and preferably encompassed within the present invention; for example, for the above experiment, the following primer pair was used as a non-limiting illustrative example only of a suitable primer pair: H53393 junc21-22F (SEQ ID NO:982) forward primer; and H53393 junc21-22R (SEQ ID NO:983) reverse primer.

The present invention also preferably encompasses any amplicon obtained through the use of any suitable primer pair; for example, for the above experiment, the following amplicon was obtained as a non-limiting illustrative example only of a suitable amplicon: H53393 junc21-22 (SEQ ID NO:984). H53393 junc21-22 Forward primer (SEQ ID NO:982): TGGTTTTTCTCTTAGTTGATTCAGACC H53393 junc21-22 Reverse primer (SEQ ID NO:983): GAGCCACTGGCTGCTTCAG H53393 junc21-22 Amplicon (SEQ ID NO:984): TGGTTTTTCTCTTAGTTGATTCAGACCTTGCATGCTGTTGACAAGGATGACCCTTATA GTGGGCACCAATTTTCGTTTTCCTTGGCCCCTGAAGCAGCCAGTGGCTC

Description for Cluster HSU40434

Cluster HSU40434 features 1 transcript(s) and 36 segment(s) of interest, the names for which are given in Tables 1 and 2, respectively, the sequences themselves are given at the end of the application. The selected protein variants are given in table 3.

TABLE 1 Transcripts of interest Transcript Name SEQ ID NO: HSU40434_PEA_1_T13 188

TABLE 2 Segments of interest Segment Name SEQ ID NO: HSU40434_PEA_1_node_1 189 HSU40434_PEA_1_node_16 190 HSU40434_PEA_1_node_30 191 HSU40434_PEA_1_node_32 192 HSU40434_PEA_1_node_57 193 H5U40434_PEA_1_node_0 194 HSU40434_PEA_1_node_10 195 HSU40434_PEA_1_node_13 196 HSU40434_PEA_1_node_18 197 HSU40434_PEA_1_node_2 198 HSU40434_PEA_1_node_20 199 HSU40434_PEA_1_node_21 200 HSU40434_PEA_1_node_23 201 HSU40434_PEA_1_node_24 202 HSU40434_PEA_1_node_26 203 HSU40434_PEA_1_node_28 204 HSU40434_PEA_1_node_3 205 HSU40434_PEA_1_node_35 206 HSU40434_PEA_1_node_36 207 HSU40434_PEA_1_node_37 208 HSU40434_PEA_1_node_38 209 HSU40434_PEA_1_node_39 210 HSU40434_PEA_1_node_40 211 HSU40434_PEA_1_node_41 212 HSU40434_PEA_1_node_42 213 HSU40434_PEA_1_node_43 214 HSU40434_PEA_1_node_44 215 HSU40434_PEA_1_node_47 216 HSU40434_PEA_1_node_48 217 HSU40434_PEA_1_node_51 218 HSU40434_PEA_1_node_52 219 HSU40434_PEA_1_node_53 220 HSU40434_PEA_1_node_54 221 HSU40434_PEA_1_node_56 222 HSU40434_PEA_1_node_7 223 HSU40434_PEA_1_node_8 224

TABLE 3 Proteins of interest Protein Name SEQ ID NO: HSU40434_PEA_1_P12 226

These sequences are variants of the known protein Mesothelin precursor (SwissProt accession identifier MSLN_HUMAN; known also according to the synonym CAK1 antigen), SEQ ID NO: 225, referred to herein as the previously known protein.

The variant proteins according to the present invention are variants of a known diagnostic marker, called Mesothelin (CAK-1).

Protein Mesothelin precursor is known or believed to have the following function(s): may play a role in cellular adhesion. Antigenic protein reactive with antibody K1. The sequence for protein Mesothelin precursor is given at the end of the application, as “Mesothelin precursor amino acid sequence”. Protein Mesothelin precursor localization is believed to be attached to the membrane by a GPI-anchor.

The following GO Annotation(s) apply to the previously known protein. The following annotation(s) were found: cell adhesion, which are annotation(s) related to Biological Process; protein binding, which are annotation(s) related to Molecular Function; and membrane, which are annotation(s) related to Cellular Component.

The GO assignment relies on information from one or more of the SwissProt/TremBl Protein knowledgebase, available from <http://www.expasy.ch/sprot/>; or Locuslink, available from <http://www.ncbi.nlm.nih.gov/projects/LocusLink/>.

Cluster HSU40434 can be used as a diagnostic marker according to overexpression of transcripts of this cluster in cancer. Expression of such transcripts in normal tissues is also given according to the previously described methods. The term “number” in the left hand column of the table and the numbers on the y-axis of FIG. 23 refer to weighted expression of ESTs in each category, as “parts per million” (ratio of the expression of ESTs for a particular cluster to the expression of all ESTs in that category, according to parts per million).

Overall, the following results were obtained as shown with regard to the histograms in FIG. 23 and Table 4. This cluster is overexpressed (at least at a minimum level) in the following pathological conditions: epithelial malignant tumors, a mixture of malignant tumors from different tissues, ovarian carcinoma and pancreas carcinoma.

TABLE 4 Normal tissue distribution Name of Tissue Number brain 2 colon 0 epithelial 9 general 4 kidney 0 liver 0 lung 32 ovary 0 pancreas 2 prostate 2 stomach 0 Thyroid 0 uterus 4

TABLE 5 P values and ratios for expression in cancerous tissue Name of Tissue P1 P2 SP1 R3 SP2 R4 brain 5.1e−01 3.1e−01 1 0.9 2.5e−01 2.7 colon 1.7e−01 1.7e−01 3.4e−01 2.4 4.6e−01 2.0 epithelial 4.3e−03 2.3e−03 9.3e−12 6.7 6.1e−08 4.5 general 4.0e−05 1.5e−05 3.9e−24 11.6 1.5e−17 7.5 kidney 4.1e−01 5.1e−01 1.1e−01 3.2 2.4e−01 2.3 liver 1 6.8e−01 1 1.0 4.8e−01 1.9 lung 5.4e−01 7.9e−01 4.8e−01 1.3 8.4e−01 0.7 ovary 8.2e−02 6.3e−02 4.8e−06 11.3 1.5e−04 8.0 pancreas 2.3e−01 8.7e−02 1.8e−04 5.4 2.4e−04 6.1 prostate 9.7e−01 9.3e−01 1 0.9 7.5e−01 1.2 stomach 1 3.0e−01 1 1.0 2.1e−01 2.3 Thyroid 5.0e−01 5.0e−01 6.7e−01 1.5 6.7e−01 1.5 uterus 9.0e−02 5.6e−02 8.5e−02 3.3 1.1e−01 2.8

As noted above, cluster HSU40434 features 1 transcript(s), which were listed in Table 1 above. These transcript(s) encode for protein(s) which are variant(s) of protein Mesothelin precursor. A description of each variant protein according to the present invention is now provided.

Variant protein HSU40434_PEA_(—)1_P12 (SEQ ID NO: 226) according to the present invention has an amino acid sequence as given at the end of the application; it is encoded by transcript(s) HSU40434_PEA_(—)1_T13 (SEQ ID NO: 188). An alignment is given to the known protein (Mesothelin precursor) at the end of the application. One or more alignments to one or more previously published protein sequences are given at the end of the application. A brief description of the relationship of the variant protein according to the present invention to each such aligned protein is as follows:

Comparison report between HSU40434_PEA_(—)1_P12 (SEQ ID NO: 226) and Q14859 (SEQ ID NO: 985) (SEQ ID NO:985):

1. An isolated chimeric polypeptide encoding for HSU40434_PEA_(—)1_P12 (SEQ ID NO: 226), comprising a first amino acid sequence being at least 90% homologous to MALPTARPLLGSCGTPALGSLLFLLFSLGWVQPSRTLAGETGQEAAPLDGVLANPPNISS LSPRQLLGFPCAEVSGLSTERVRELAVALAQKNVKLSTEQLRCLAHRLSEPPEDLDALP LDLLLFLNPDAFSGPQACTRFFSRITKANVDLLPRGAPERQRLLPAALACWGVRGSLLS EADVRALGGLACDLPGRFVAESAEVLLPRLVSCPGPLDQDQQEAARAALQGGGPPYGP PSTWSVSTMDALRGLLPVLGQPIIRSIPQGIVAAWRQRSSRDPSWRQPERTILRPRFRRE VEKTACPSGKKAREIDESLIFYKKWELEACVDAALLATQMDRVNAIPFTYEQLDVLKH KLDELYPQGYPESVIQHLGYLFLKMSPEDIRKWNVTSLETLKALLEVNKGHEMSPQVA TLIDRFVKGRGQLDKDTLDTLTAFYPGYLCSLSPEELSSVPPSSIW corresponding to amino acids 1-458 of Q14859 (SEQ ID NO: 985), which also corresponds to amino acids 1-458 of HSU40434_PEA_(—)1_P12 (SEQ ID NO: 226).

Comparison report between HSU40434_PEA_(—)1_P12 (SEQ ID NO: 226) and Q9BTR2 (SEQ ID NO: 986) (SEQ ID NO:986):

1. An isolated chimeric polypeptide encoding for HSU40434_PEA_(—)1_P12 (SEQ ID NO: 226), comprising a first amino acid sequence being at least 90% homologous to MALPTARPLLGSCGTPALGSLLFLLFSLGWVQPSRTLAGETGQ corresponding to amino acids 1-43 of Q9BTR2 (SEQ ID NO: 986), which also corresponds to amino acids 1-43 of HSU40434_PEA_(—)1_P12 (SEQ ID NO: 226), second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence E corresponding to amino acids 44-44 of HSU40434_PEA_(—)1_P12 (SEQ ID NO: 226), and a third amino acid sequence being at least 90% homologous to AAPLDGVLANPPNISSLSPRQLLGFPCAEVSGLSTERVRELAVALAQKNVKLSTEQLRC LAHRLSEPPEDLDALPLDLLLFLNPDAFSGPQACTRFFSRITKANVDLLPRGAPERQRLL PAALACWGVRGSLLSEADVRALGGLACDLPGRFVAESAEVLLPRLVSCPGPLDQDQQE AARAALQGGGPPYGPPSTWSVSTMDALRGLLPVLGQPIIRSIPQGIVAAWRQRSSRDPS WRQPERTILRPRFRREVEKTACPSGKKAREIDESLIFYKKWELEACVDAALLATQMDRV NAIPFTYEQLDVLKHKLDELYPQGYPESVIQHLGYLFLKMSPEDIRKWNVTSLETLKAL LEVNKGHEMSPQVATLIDRFVKGRGQLDKDTLDTLTAFYPGYLCSLSPEELSSVPPSSIW corresponding to amino acids 44-457 of Q9BTR2 (SEQ ID NO: 986), which also corresponds to amino acids 45-458 of HSU40434_PEA_(—)1_P12 (SEQ ID NO: 226), wherein said first, second and third amino acid sequences are contiguous and in a sequential order.

2. An isolated polypeptide encoding for an edge portion of HSU40434_PEA_(—)1_P12 (SEQ ID NO: 226), comprising an amino acid sequence being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence encoding for E, corresponding to HSU40434_PEA_(—)1_P12 (SEQ ID NO: 226).

The location of the variant protein was determined according to results from a number of different software programs and analyses, including analyses from SignalP and other specialized programs. The variant protein is believed to be located as follows with regard to the cell: secreted. The protein localization is believed to be secreted because both signal-peptide prediction programs predict that this protein has a signal peptide, and neither trans-membrane region prediction program predicts that this protein has a trans-membrane region.

Variant protein HSU40434_PEA_(—)1_P12 (SEQ ID NO: 226) also has the following non-silent SNPs (Single Nucleotide Polymorphisms) as listed in Table 6, (given according to their position(s) on the amino acid sequence, with the alternative amino acid(s) listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein HSU40434_PEA_(—)1_P12 (SEQ ID NO: 226) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 6 Amino acid mutations SNP position(s) on amino acid Previously sequence Alternative amino acid(s) known SNP? 118 L -> V No 139 R -> H No 162 L -> Q No 235 G -> No 330 A -> V No 342 I -> N No 402 N -> D No 51 V -> No

Variant protein HSU40434_PEA_(—)1_P12 (SEQ ID NO: 226) is encoded by the following transcript(s): HSU40434_PEA_(—)1_T13 (SEQ ID NO: 188), for which the sequence(s) is/are given at the end of the application. The coding portion of transcript HSU40434_PEA_(—)1_T13 (SEQ ID NO: 188) is shown in bold; this coding portion starts at position 420 and ends at position 1793. The transcript also has the following SNPs as listed in Table 7 (given according to their position on the nucleotide sequence, with the alternative nucleic acid listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein HSU40434_PEA_(—)1_P12 (SEQ ID NO: 226) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 7 Nucleic acid SNPs SNP position on nucleotide Previously sequence Alternative nucleic acid known SNP? 170 G -> A Yes 334 G -> A Yes 1623 A -> G No 1931 G -> No 1955 A -> G No 2270 A -> G No 2352 C -> No 2431 G -> A No 2482 C -> A No 2483 C -> A No 557 G -> A No 572 C -> No 771 C -> G No 835 G -> A No 904 T -> A No 1124 C -> No 1408 C -> T No 1444 T -> A No

As noted above, cluster HSU40434 features 36 segment(s), which were listed in Table 2 above and for which the sequence(s) are given at the end of the application. These segment(s) are portions of nucleic acid sequence(s) which are described herein separately because they are of particular interest. A description of each segment according to the present invention is now provided.

Segment cluster HSU40434_PEA_(—)1_node_(—)1 (SEQ ID NO: 189) according to the present invention is supported by 3 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HSU40434_PEA_(—)1_T13 (SEQ ID NO: 188). Table 8 below describes the starting and ending position of this segment on each transcript.

TABLE 8 Segment location on transcripts Segment start- Segment Transcript name ing position ending position HSU40434_PEA_1_T13 58 308 (SEQ ID NO: 188)

Segment cluster HSU40434_PEA_(—)1_node_(—)16 (SEQ ID NO: 190) according to the present invention is supported by 30 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HSU40434_PEA_(—)1_T13 (SEQ ID NO: 188). Table 9 below describes the starting and ending position of this segment on each transcript.

TABLE 9 Segment location on transcripts Segment start- Segment Transcript name ing position ending position HSU40434_PEA_1_T13 599 719 (SEQ ID NO: 188)

Segment cluster HSU40434_PEA_(—)1_node_(—)30 (SEQ ID NO: 191) according to the present invention is supported by 37 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HSU40434_PEA_(—)1_T13 (SEQ ID NO: 188). Table 10 below describes the starting and ending position of this segment on each transcript.

TABLE 10 Segment location on transcripts Segment start- Segment Transcript name ing position ending position HSU40434_PEA_1_T13 1315 1493 (SEQ ID NO: 188)

Segment cluster HSU40434_PEA_(—)1_node_(—)32 (SEQ ID NO: 192) according to the present invention is supported by 45 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HSU40434_PEA_(—)1_T13 (SEQ ID NO: 188). Table 11 below describes the starting and ending position of this segment on each transcript.

TABLE 11 Segment location on transcripts Segment start- Segment Transcript name ing position ending position HSU40434_PEA_1_T13 1494 1649 (SEQ ID NO: 188)

Segment cluster HSU40434_PEA_(—)1_node_(—)57 (SEQ ID NO: 193) according to the present invention is supported by 53 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HSU40434_PEA_(—)1_T13 (SEQ ID NO: 188). Table 12 below describes the starting and ending position of this segment on each transcript.

TABLE 12 Segment location on transcripts Segment start- Segment Transcript name ing position ending position HSU40434_PEA_1_T13 2307 2499 (SEQ ID NO: 188)

According to an optional embodiment of the present invention, short segments related to the above cluster are also provided. These segments are up to about 120 bp in length, and so are included in a separate description.

Segment cluster HSU40434_PEA_(—)1_node_(—)0 (SEQ ID NO: 194) according to the present invention is supported by 4 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HSU40434_PEA_(—)1_T13 (SEQ ID NO: 188). Table 13 below describes the starting and ending position of this segment on each transcript.

TABLE 13 Segment location on transcripts Segment start- Segment Transcript name ing position ending position HSU40434_PEA_1_T13 1 57 (SEQ ID NO: 188)

Segment cluster HSU40434_PEA_(—)1_node_(—)10 (SEQ ID NO: 195) according to the present invention is supported by 28 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HSU40434_PEA_(—)1_T13 (SEQ ID NO: 188). Table 14 below describes the starting and ending position of this segment on each transcript.

TABLE 14 Segment location on transcripts Segment Segment Transcript starting ending name position position HSU40434_PEA_1_T13 505 548 (SEQ ID NO:188)

Segment cluster HSU40434_PEA_(—)1_node_(—)13 (SEQ ID NO: 196) according to the present invention is supported by 27 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HSU40434_PEA_(—)1_T13 (SEQ ID NO: 188). Table 15 below describes the starting and ending position of this segment on each transcript.

TABLE 15 Segment location on transcripts Segment Segment Transcript starting ending name position position HSU40434_PEA_1_T13 549 598 (SEQ ID NO:188)

Segment cluster HSU40434_PEA_(—)1_node_(—)18 (SEQ ID NO: 197) according to the present invention is supported by 30 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HSU40434_PEA_(—)1_T13 (SEQ ID NO: 188). Table 16 below describes the starting and ending position of this segment on each transcript.

TABLE 16 Segment location on transcripts Segment Segment Transcript starting ending name position position HSU40434_PEA_1_T13 720 799 (SEQ ID NO:188)

Segment cluster HSU40434_PEA_(—)1_node_(—)2 (SEQ ID NO: 198) according to the present invention is supported by 11 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HSU40434_PEA_(—)1_T13 (SEQ ID NO: 188). Table 17 below describes the starting and ending position of this segment on each transcript.

TABLE 17 Segment location on transcripts Segment Segment Transcript starting ending name position position HSU40434_PEA_1_T13 309 368 (SEQ ID NO:188)

Segment cluster HSU40434_PEA_(—)1_node_(—)20 (SEQ ID NO: 199) according to the present invention is supported by 29 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HSU40434_PEA_(—)1_T13 (SEQ ID NO: 188). Table 18 below describes the starting and ending position of this segment on each transcript.

TABLE 18 Segment location on transcripts Segment Segment Transcript starting ending name position position HSU40434_PEA_1_T13 800 905 (SEQ ID NO:188)

Segment cluster HSU40434_PEA_(—)1_node_(—)21 (SEQ ID NO: 200) according to the present invention can be found in the following transcript(s): HSU40434_PEA_(—)1_T13 (SEQ ID NO: 188). Table 19 below describes the starting and ending position of this segment on each transcript.

TABLE 19 Segment location on transcripts Segment Segment Transcript starting ending name position position HSU40434_PEA_1_T13 906 929 (SEQ ID NO:188)

Segment cluster HSU40434_PEA_(—)1_node_(—)23 (SEQ ID NO: 201) according to the present invention is supported by 27 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HSU40434_PEA_(—)1_T13 (SEQ ID NO: 188). Table 20 below describes the starting and ending position of this segment on each transcript.

TABLE 20 Segment location on transcripts Segment Segment Transcript starting ending name position position HSU40434_PEA_1_T13 930 1043 (SEQ ID NO:188)

Segment cluster HSU40434_PEA_(—)1_node_(—)24 (SEQ ID NO: 202) according to the present invention is supported by 26 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HSU40434_PEA_(—)1_T13 (SEQ ID NO: 188). Table 21 below describes the starting and ending position of this segment on each transcript.

TABLE 21 Segment location on transcripts Segment Segment Transcript starting ending name position position HSU40434_PEA_1_T13 1044 1123 (SEQ ID NO:188)

Segment cluster HSU40434_PEA_(—)1_node_(—)26 (SEQ ID NO: 203) according to the present invention is supported by 28 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HSU40434_PEA_(—)1_T13 (SEQ ID NO: 188). Table 22 below describes the starting and ending position of this segment on each transcript.

TABLE 22 Segment location on transcripts Segment Segment Transcript starting ending name position position HSU40434_PEA_1_T13 1124 1214 (SEQ ID NO:188)

Segment cluster HSU40434_PEA_(—)1_node_(—)28 (SEQ ID NO: 204) according to the present invention is supported by 30 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HSU40434_PEA_(—)1_T13 (SEQ ID NO: 188). Table 23 below describes the starting and ending position of this segment on each transcript.

TABLE 23 Segment location on transcripts Segment Segment Transcript starting ending name position position HSU40434_PEA_1_T13 1215 1314 (SEQ ID NO:188)

Segment cluster HSU40434_PEA_(—)1_node_(—)3 (SEQ ID NO: 205) according to the present invention is supported by 19 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HSU40434_PEA_(—)1_T13 (SEQ ID NO: 188). Table 24 below describes the starting and ending position of this segment on each transcript.

TABLE 24 Segment location on transcripts Segment Segment Transcript starting ending name position position HSU40434_PEA_1_T13 369 410 (SEQ ID NO:188)

Segment cluster HSU40434_PEA_(—)1_node_(—)35 (SEQ ID NO: 206) according to the present invention is supported by 43 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HSU40434_PEA_(—)1_T13 (SEQ ID NO: 188). Table 25 below describes the starting and ending position of this segment on each transcript.

TABLE 25 Segment location on transcripts Segment Segment Transcript starting ending name position position HSU40434_PEA_1_T13 1650 1679 (SEQ ID NO:188)

Segment cluster HSU40434_PEA_(—)1_node_(—)36 (SEQ ID NO: 207) according to the present invention is supported by 51 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HSU40434_PEA_(—)1_T13 (SEQ ID NO: 188). Table 26 below describes the starting and ending position of this segment on each transcript.

TABLE 26 Segment location on transcripts Segment Segment Transcript starting ending name position position HSU40434_PEA_1_T13 1680 1753 (SEQ ID NO:188)

Segment cluster HSU40434_PEA_(—)1_node_(—)37 (SEQ ID NO: 208) according to the present invention is supported by 48 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HSU40434_PEA_(—)1_T13 (SEQ ID NO: 188). Table 27 below describes the starting and ending position of this segment on each transcript.

TABLE 27 Segment location on transcripts Segment Segment Transcript starting ending name position position HSU40434_PEA_1_T13 1754 1792 (SEQ ID NO:188)

Segment cluster HSU40434_PEA_(—)1_node_(—)38 (SEQ ID NO: 209) according to the present invention is supported by 1 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HSU40434_PEA_(—)1_T13 (SEQ ID NO: 188). Table 28 below describes the starting and ending position of this segment on each transcript.

TABLE 28 Segment location on transcripts Segment Segment Transcript starting ending name position position HSU40434_PEA_1_T13 1793 1866 (SEQ ID NO:188)

Segment cluster HSU40434_PEA_(—)1_node_(—)39 (SEQ ID NO: 210) according to the present invention is supported by 46 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HSU40434_PEA_(—)1_T13 (SEQ ID NO: 188). Table 29 below describes the starting and ending position of this segment on each transcript.

TABLE 29 Segment location on transcripts Segment Segment Transcript starting ending name position position HSU40434_PEA_1_T13 1867 1909 (SEQ ID NO:188)

Segment cluster HSU40434_PEA_(—)1_node_(—)40 (SEQ ID NO: 211) according to the present invention can be found in the following transcript(s): HSU40434_PEA_(—)1_T13 (SEQ ID NO: 188). Table 30 below describes the starting and ending position of this segment on each transcript.

TABLE 30 Segment location on transcripts Segment Segment Transcript starting ending name position position HSU40434_PEA_1_T13 1910 1930 (SEQ ID NO:188)

Segment cluster HSU40434_PEA_(—)1_node_(—)41 (SEQ ID NO: 212) according to the present invention can be found in the following transcript(s): HSU40434_PEA_(—)1_T13 (SEQ ID NO: 188). Table 31 below describes the starting and ending position of this segment on each transcript.

TABLE 31 Segment location on transcripts Segment Segment Transcript starting ending name position position HSU40434_PEA_1_T13 1931 1948 (SEQ ID NO:188)

Segment cluster HSU40434_PEA_(—)1_node_(—)42 (SEQ ID NO: 213) according to the present invention can be found in the following transcript(s): HSU40434_PEA_(—)1_T13 (SEQ ID NO: 188). Table 32 below describes the starting and ending position of this segment on each transcript.

TABLE 32 Segment location on transcripts Segment Segment Transcript starting ending name position position HSU40434_PEA_1_T13 1949 1972 (SEQ ID NO:188)

Segment cluster HSU40434_PEA_(—)1_node_(—)43 (SEQ ID NO: 214) according to the present invention can be found in the following transcript(s): HSU40434_PEA_(—)1_T13 (SEQ ID NO: 188). Table 33 below describes the starting and ending position of this segment on each transcript.

TABLE 33 Segment location on transcripts Segment Segment Transcript starting ending name position position HSU40434_PEA_1_T13 1973 1990 (SEQ ID NO:188)

Segment cluster HSU40434_PEA_(—)1_node_(—)44 (SEQ ID NO: 215) according to the present invention can be found in the following transcript(s): HSU40434_PEA_(—)1_T13 (SEQ ID NO: 188). Table 34 below describes the starting and ending position of this segment on each transcript.

TABLE 34 Segment location on transcripts Segment Segment Transcript starting ending name position position HSU40434_PEA_1_T13 1991 1994 (SEQ ID NO:188)

Segment cluster HSU40434_PEA_(—)1_node_(—)47 (SEQ ID NO: 216) according to the present invention is supported by 49 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HSU40434_PEA_(—)1_T13 (SEQ ID NO: 188). Table 35 below describes the starting and ending position of this segment on each transcript.

TABLE 35 Segment location on transcripts Segment Segment Transcript starting ending name position position HSU40434_PEA_1_T13 1995 2032 (SEQ ID NO:188)

Segment cluster HSU40434_PEA_(—)1_node_(—)48 (SEQ ID NO: 217) according to the present invention is supported by 50 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HSU40434_PEA_(—)1_T13 (SEQ ID NO: 188). Table 36 below describes the starting and ending position of this segment on each transcript.

TABLE 36 Segment location on transcripts Segment Segment Transcript starting ending name position position HSU40434_PEA_1_T13 2033 2089 (SEQ ID NO:188)

Segment cluster HSU40434_PEA_(—)1_node_(—)51 (SEQ ID NO: 218) according to the present invention can be found in the following transcript(s): HSU40434_PEA_(—)1_T13 (SEQ ID NO: 188). Table 37 below describes the starting and ending position of this segment on each transcript.

TABLE 37 Segment location on transcripts Segment Segment Transcript starting ending name position position HSU40434_PEA_1_T13 2090 2113 (SEQ ID NO:188)

Segment cluster HSU40434_PEA_(—)1_node_(—)52 (SEQ ID NO: 219) according to the present invention is supported by 52 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HSU40434_PEA_(—)1_T13 (SEQ ID NO: 188). Table 38 below describes the starting and ending position of this segment on each transcript.

TABLE 38 Segment location on transcripts Segment Segment Transcript starting ending name position position HSU40434_PEA_1_T13 2114 2140 (SEQ ID NO:188)

Segment cluster HSU40434_PEA_(—)1_node_(—)53 (SEQ ID NO: 220) according to the present invention is supported by 58 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HSU40434_PEA_(—)1_T13 (SEQ ID NO: 188). Table 39 below describes the starting and ending position of this segment on each transcript.

TABLE 39 Segment location on transcripts Segment Segment Transcript starting ending name position position HSU40434_PEA_1_T13 2141 2197 (SEQ ID NO:188)

Segment cluster HSU40434_PEA_(—)1_node_(—)54 (SEQ ID NO: 221) according to the present invention is supported by 56 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HSU40434_PEA_(—)1_T13 (SEQ ID NO: 188). Table 40 below describes the starting and ending position of this segment on each transcript.

TABLE 40 Segment location on transcripts Segment Segment Transcript starting ending name position position HSU40434_PEA_1_T13 2198 2276 (SEQ ID NO:188)

Segment cluster HSU40434_PEA_(—)1_node_(—)56 (SEQ ID NO: 222) according to the present invention is supported by 49 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HSU40434_PEA_(—)1_T13 (SEQ ID NO: 188). Table 41 below describes the starting and ending position of this segment on each transcript.

TABLE 41 Segment location on transcripts Segment Segment Transcript starting ending name position position HSU40434_PEA_1_T13 2277 2306 (SEQ ID NO:188)

Segment cluster HSU40434_PEA_(—)1_node_(—)7 (SEQ ID NO: 223) according to the present invention is supported by 29 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HSU40434_PEA_(—)1_T13 (SEQ ID NO: 188). Table 42 below describes the starting and ending position of this segment on each transcript.

TABLE 42 Segment location on transcripts Segment Segment Transcript starting ending name position position HSU40434_PEA_1_T13 411 464 (SEQ ID NO:188)

Segment cluster HSU40434_PEA_(—)1_node_(—)8 (SEQ ID NO: 224) according to the present invention is supported by 28 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HSU40434_PEA_(—)1_T13 (SEQ ID NO: 188). Table 43 below describes the starting and ending position of this segment on each transcript.

TABLE 43 Segment location on transcripts Segment Segment Transcript starting ending name position position HSU40434_PEA_1_T13 465 504 (SEQ ID NO:188) Variant protein alignment to the previously known protein: Sequence name: /tmp/tZTolp1A91/eTMhjqGV2R:Q14859 (SEQ ID NO: 985) Sequence documentation:

Alignment of: HSU40434_PEA_(—)1_P12 (SEQ ID NO: 226) x Q14859 (SEQ ID NO: 985) ..

Alignment segment 1/1:

Quality: 4448.00 Escore: 0 Matching length: 458 Total length: 458 Matching Percent Similarity: 100.00 Matching Percent Identity: 100.00 Total Percent Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0

Alignment:

Sequence name: /tmp/tZTolp1A91/eTMhjqGV2R:Q9BTR2 (SEQ ID NO: 986) Sequence documentation:

Alignment of: HSU40434_PEA_(—)1_P12 (SEQ ID NO: 226) x Q9BTR2 (SEQ ID NO: 986) ..

Alignment segment 1/1:

Quality: 4338.00 Escore: 0 Matching length: 457 Total length: 458 Matching Percent Similarity: 100.00 Matching Percent Identity: 100.00 Total Percent Similarity: 99.78 Total Percent Identity: 99.78 Gaps: 1

Alignment:

Sequence name: /tmp/tZTolp1A91/eTMhjqGV2R:MSLN_HUMAN Sequence documentation:

Alignment of: HSU40434_PEA_(—)1_P12 (SEQ ID NO: 226) x MSLN_HUMAN ..

Alignment segment 1/1:

Quality: 4074.00 Escore: 0 Matching length: 440 Total length: 448 Matching Percent Similarity: 98.86 Matching Percent Identity: 97.95 Total Percent Similarity: 97.10 Total Percent Identity: 96.21 Gaps: 1

Alignment:

Description for Cluster M77904

Cluster M77904 features 4 transcript(s) and 21 segment(s) of interest, the names for which are given in Tables 1 and 2, respectively, the sequences themselves are given at the end of the application. The selected protein variants are given in table 3.

TABLE 1 Transcripts of interest Transcript Name SEQ ID NO: M77904_T11 227 M77904_T3 228 M77904_T8 229 M77904_T9 230

TABLE 2 Segments of interest Segment Name SEQ ID NO: M77904_node_0 231 M77904_node_11 232 M77904_node_12 233 M77904_node_14 234 M77904_node_15 235 M77904_node_17 236 M77904_node_2 237 M77904_node_21 238 M77904_node_23 239 M77904_node_24 240 M77904_node_27 241 M77904_node_28 242 M77904_node_4 243 M77904_node_6 244 M77904_node_7 245 M77904_node_8 246 M77904_node_9 247 M77904_node_19 248 M77904_node_22 249 M77904_node_25 250 M77904_node_26 251

TABLE 3 Proteins of interest Protein Name SEQ ID NO: M77904_P2 252 M77904_P4 253 M77904_P5 254 M77904_P7 255

Cluster M77904 can be used as a diagnostic marker according to overexpression of transcripts of this cluster in cancer. Expression of such transcripts in normal tissues is also given according to the previously described methods. The term “number” in the left hand column of the table and the numbers on the y-axis of FIG. 24 refer to weighted expression of ESTs in each category, as “parts per million” (ratio of the expression of ESTs for a particular cluster to the expression of all ESTs in that category, according to parts per million).

Overall, the following results were obtained as shown with regard to the histograms in FIG. 24 and Table 4. This cluster is overexpressed (at least at a minimum level) in the following pathological conditions: a mixture of malignant tumors from different tissues.

TABLE 4 Normal tissue distribution Name of Tissue Number bladder 0 brain 0 colon 94 epithelial 35 general 15 kidney 0 liver 0 lung 33 breast 140 bone marrow 0 ovary 0 pancreas 26 prostate 94 stomach 36 Thyroid 0 uterus 22

TABLE 5 P values and ratios for expression in cancerous tissue Name of Tissue P1 P2 SP1 R3 SP2 R4 bladder 5.4e−01 3.4e−01 5.6e−01 1.8 3.2e−01 2.4 brain 8.8e−02 1.3e−01 4.8e−02 8.1 1.1e−01 5.1 colon 3.8e−01 3.8e−01 8.7e−01 0.8 8.2e−01 0.8 epithelial 3.1e−02 1.5e−02 4.9e−01 1.1 3.9e−02 1.4 general 2.0e−04 3.4e−05 4.1e−03 2.0 6.2e−07 2.5 kidney 6.5e−01 3.5e−01 1 1.1 1.4e−02 4.0 liver 1 3.0e−01 1 1.0 2.3e−01 2.0 lung 5.9e−01 4.8e−01 8.8e−01 0.7 3.4e−01 1.2 breast 8.7e−01 8.8e−01 1 0.2 9.4e−01 0.3 bone marrow 1 4.2e−01 1 1.0 5.3e−01 2.1 ovary 1.3e−01 9.4e−02 3.2e−01 2.4 3.4e−01 2.2 pancreas 5.1e−01 5.2e−01 2.1e−01 1.8 7.6e−02 1.8 prostate 8.6e−01 8.0e−01 9.2e−01 0.5 8.4e−01 0.6 stomach 2.7e−01 1.9e−01 5.0e−01 1.5 2.7e−01 1.8 Thyroid 6.4e−01 6.4e−01 6.7e−01 1.5 6.7e−01 1.5 uterus 1.2e−01 3.4e−01 5.9e−01 1.4 8.2e−01 0.9

As noted above, cluster M77904 features 4 transcript(s), which were listed in Table 1 above. A description of each variant protein according to the present invention is now provided.

Variant protein M77904_P2 (SEQ ID NO: 252) according to the present invention has an amino acid sequence as given at the end of the application; it is encoded by transcript(s) M77904_T3 (SEQ ID NO: 228). One or more alignments to one or more previously published protein sequences are given at the end of the application. A brief description of the relationship of the variant protein according to the present invention to each such aligned protein is as follows:

Comparison report between M77904_P2 (SEQ ID NO: 252) and Q8WU91 (SEQ ID NO: 987) (SEQ ID NO:987):

1. An isolated chimeric polypeptide encoding for M77904_P2 (SEQ ID NO: 252), comprising a first amino acid sequence being at least 90% homologous to MLSIKSGERIVFTFSCQSPENHFVIEIQKNIDCMSGPCPFGEVQLQPSTSLLPTLNRTFIWD VKAHKSIGLELQFSIPRLRQIGPGESCPDGVTHSISGRIDATVVRIGTFCSNGTVSRIKMQ EGVKMALHLPWFHPRNVSGFSIANRSSIKRLCIIESVFEGEGSATLMSANYPEGFPEDEL MTWQFVVPAHLRASVSFLNFNLSNCERKEERVEYYIPGSTTNPEVFKLEDKQPGNMAG NFNLSLQGCDQDAQSPGILRLQFQVLVQHPQNES corresponding to amino acids 67-341 of Q8WU91 (SEQ ID NO: 987), which also corresponds to amino acids 1-275 of M77904_P2 (SEQ ID NO: 252), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence NKIYVVDLSNERAMSLTIEPRPVKQSRKFVPGCFVCLESRTCSSNLTLTSGSKHKISFLCD DLTRLWMNVEKTISCTDHRYCQRKSYSLQVPSDILHLPVELHDFSWKLLVPKDRLSLVL VPAQKLQQHTHEKPCNTSFSYLVASAIPSQDLYFGSFCPGGSIKQIQVKQNISVTLRTFAP SFQQEASRQGLTVSFIPYFKEEGVFTVTPDTKSKVYLRTPNWDRGLPSLTSVSWNISVPR DQVACLTFFKERSGVVCQTGRAFMIIQEQRTRAEEIFSLDEDVLPKPSFHHHSFWVNISN CSPTSGKQLDLLFSVTLTPRTVDLTVILIAAVGGGVLLLSALGLIICCVKKKKKKTNKGP AVGIYNGNINTEMPRQPKKFQKGRKDNDSHVYAVIEDTMVYGHLLQDSSGSFLQPEVD TYRPFQGTMGVCPPSPPTICSRAPTAKLATEEPPPRSPPESESEPYTFSHPNNGDVSSKDT DIPLLNTQEPMEPAE (SEQ ID NO: 1135) corresponding to amino acids 276-770 of M77904_P2 (SEQ ID NO: 252), wherein said first and second amino acid sequences are contiguous and in a sequential order.

2. An isolated polypeptide encoding for a tail of M77904_P2 (SEQ ID NO: 252), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence NKIYVVDLSNERAMSLTIEPRPVKQSRKFVPGCFVCLESRTCSSNLTLTSGSKHKISFLCD DLTRLWMNVEKTISCTDHRYCQRKSYSLQVPSDILHLPVELHDFSWKLLVPKDRLSLVL VPAQKLQQHTHEKPCNTSFSYLVASAIPSQDLYFGSFCPGGSIKQIQVKQNISVTLRTFAP SFQQEASRQGLTVSFIPYFKEEGVFTVTPDTKSKVYLRTPNWDRGLPSLTSVSWNISVPR DQVACLTFFKERSGVVCQTGRAFMIIQEQRTRAEEIFSLDEDVLPKPSFHHHSFWVNISN CSPTSGKQLDLLFSVTLTPRTVDLTVILIAAVGGGVLLLSALGLIICCVKKKKKKTNKGP AVGIYNGNINTEMPRQPKKFQKGRKDNDSHVYAVIEDTMVYGHLLQDSSGSFLQPEVD TYRPFQGTMGVCPPSPPTICSRAPTAKLATEEPPPRSPPESESEPYTFSHPNNGDVSSKDT DIPLLNTQEPMEPAE (SEQ ID NO: 1135) in M77904_P2 (SEQ ID NO: 252).

Comparison report between M77904_P2 (SEQ ID NO: 252) and Q96QU7 (SEQ ID NO: 988) (SEQ ID NO:988):

1. An isolated chimeric polypeptide encoding for M77904_P2 (SEQ ID NO: 252), comprising a first amino acid sequence being at least 90% homologous to MLSIKSGERIVFTFSCQSPENHFVIEIQKNIDCMSGPCPFGEVQLQPSTSLLPTLNRTFIWD VKAHKSIGLELQFSIPRLRQIGPGESCPDGVTHSISGRIDATVVRIGTFCSNGTVSRIKMQ EGVKMALHLPWFHPRNVSGFSIANRSSIKRLCIIESVFEGEGSATLMSANYPEGFPEDEL MTWQFVVPAHLRASVSFLNFNLSNCERKEERVEYYIPGSTTNPEVFKLEDKQPGNMAG NFNLSLQGCDQDAQSPGILRLQFQVLVQHPQNESNKIYVVDLSNERAMSLTIEPRPVKQ SRKFVPGCFVCLESRTCSSNLTLTSGSKHKISFLCDDLTRLWMNVEKTISCTDHRYCQR KSYSLQVPSDILHLPVELHDFSWKLLVPKDRLSLVLVPAQKLQQHTHEKPCNTSFSYLV ASAIPSQDLYFGSFCPGGSIKQIQVKQNISVTLRTFAPSFQQEASRQGLTVSFIPYFKEEGV FTVTPDTKSKVYLRTPNWDRGLPSLTSVSWNISVPRDQVACLTFFKERSGVVCQTGRAF MIIQEQRTRAEEIFSLDEDVLPKPSFHHHSFWVNISNCSPTSGKQLDLLFSVTLTPRTVDL TVILIAAVGGGVLLLSALGLIICCVKKKKKKTNKGPAVGIYNGNINTEMPRQPKKFQKG RKDNDSHVYAVIEDTMVYGHLLQDSSGSFLQPEVDTYRPFQGTMGVCPPSPPTICSRAP TAKLATEEPPPRSPPESESEPYTFSHPNNGDVSSKDTDIPLLNTQEPMEPAE (SEQ ID NO: 1135) corresponding to amino acids 67-836 of Q96QU7 (SEQ ID NO: 988), which also corresponds to amino acids 1-770 of M77904_P2 (SEQ ID NO: 252).

The location of the variant protein was determined according to results from a number of different software programs and analyses, including analyses from SignalP and other specialized programs. The variant protein is believed to be located as follows with regard to the cell: membrane. The protein localization is believed to be membrane because both trans-membrane region prediction programs predicted a trans-membrane region for this protein. In addition both signal-peptide prediction programs predict that this protein is a non-secreted protein.

Variant protein M77904_P2 (SEQ ID NO: 252) also has the following non-silent SNPs (Single Nucleotide Polymorphisms) as listed in Table 6, (given according to their position(s) on the amino acid sequence, with the alternative amino acid(s) listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein M77904_P2 (SEQ ID NO: 252) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 6 Amino acid mutations SNP position(s) on amino acid Previously sequence Alternative amino acid(s) known SNP? 263 Q -> R No 459 Q -> R Yes 643 G -> D Yes

Variant protein M77904_P2 (SEQ ID NO: 252) is encoded by the following transcript(s): M77904_T3 (SEQ ID NO: 228), for which the sequence(s) is/are given at the end of the application. The coding portion of transcript M77904_T3 (SEQ ID NO: 228) is shown in bold; this coding portion starts at position 238 and ends at position 2547. The transcript also has the following SNPs as listed in Table 7 (given according to their position on the nucleotide sequence, with the alternative nucleic acid listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein M77904_P2 (SEQ ID NO: 252) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 7 Nucleic acid SNPs SNP position on nucleotide Previously sequence Alternative nucleic acid known SNP? 561 C -> T No 585 T -> C No 3276 T -> G Yes 3465 C -> T Yes 3760 A -> T Yes 3830 G -> A Yes 3900 A -> G Yes 3960 C -> A Yes 4114 G -> A Yes 4613 C -> T Yes 5050 G -> A No 5309 A -> C Yes 957 G -> A Yes 5329 A -> G Yes 5420 T -> C Yes 5490 T -> C Yes 5507 C -> A Yes 5511 G -> A Yes 5578 T -> G Yes 5662 A -> C No 1025 A -> G No 1613 A -> G Yes 1623 C -> T Yes 2085 T -> C No 2165 G -> A Yes 3043 T -> C No 3122 G -> A Yes

Variant protein M77904_P4 (SEQ ID NO: 253) according to the present invention has an amino acid sequence as given at the end of the application; it is encoded by transcript(s) M77904_T8 (SEQ ID NO: 229). One or more alignments to one or more previously published protein sequences are given at the end of the application. A brief description of the relationship of the variant protein according to the present invention to each such aligned protein is as follows:

Comparison report between M77904_P4 (SEQ ID NO: 253) and Q8WU91 (SEQ ID NO: 987):

1. An isolated chimeric polypeptide encoding for M77904_P4 (SEQ ID NO: 253), comprising a first amino acid sequence being at least 90% homologous to MAGLNCGVSIALLGVLLLGAARLPRGAEAFEIALPRESNITVLIKLGTPTLLAKPCYIVIS KRHITMLSIKSGERIVFTFSCQSPENHFVIEIQKNIDCMSGPCPFGEVQLQPSTSLLPTLNR TFIWDVKAHKSIGLELQFSIPRLRQIGPGESCPDGVTHSISGRIDATVVRIGTFCSNGTVSR IKMQEGVKMALHLPWFHPRNVSGFSIANRSSIKRLCIIESVFEGEGSATLMSANYPEGFP EDELMTWQFVVPAHLRASVSFLNFNLSNCERKEERVEYYIPGSTTNPEVFKLEDKQPGN MAGNFNLSLQGCDQDAQSPGILRLQFQVLVQHPQNES corresponding to amino acids 1-341 of Q8WU91 (SEQ ID NO: 987), which also corresponds to amino acids 1-341 of M77904_P4 (SEQ ID NO: 253), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence NKIYVVDLSNERAMSLTIEPRPVKQSRKFVPGCFVCLESRTCSSNLTLTSGSKHKISFLCD DLTRLWMNVEKTISTPLNQCICPWPWIALLSPPCLSGVPWVGCKSYQKGPSGRARWLT PVIPALWEAKAGGSLEVRSSRPAWPTW (SEQ ID NO: 1136) corresponding to amino acids 342-487 of M77904_P4 (SEQ ID NO: 253), wherein said first and second amino acid sequences are contiguous and in a sequential order.

2. An isolated polypeptide encoding for a tail of M77904_P4 (SEQ ID NO: 253), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence NKIYVVDLSNERAMSLTIEPRPVKQSRKFVPGCFVCLESRTCSSNLTLTSGSKHKISFLCD DLTRLWMNVEKTISTPLNQCICPWPWIALLSPPCLSGVPWVGCKSYQKGPSGRARWLT PVIPALWEAKAGGSLEVRSSRPAWPTW (SEQ ID NO: 1136) in M77904_P4 (SEQ ID NO: 253).

Comparison report between M77904_P4 (SEQ ID NO: 253) and Q9H5V8 (SEQ ID NO: 989) (SEQ ID NO:989):

1. An isolated chimeric polypeptide encoding for M77904_P4 (SEQ ID NO: 253), comprising a first amino acid sequence being at least 90% homologous to MAGLNCGVSIALLGVLLLGAARLPRGAEAFEIALPRESNITVLIKLGTPTLLAKPCYIVIS KRHITMLSIKSGERIVFTFSCQSPENHFVIEIQKNIDCMSGPCPFGEVQLQPSTSLLPTLNR TFIWDVKAHKSIGLELQFSIPRLRQIGPGESCPDGVTHSISGRIDATVVRIGTFCSNGTVSR IKMQEGVKMALHLPWFHPRNVSGFSIANRSSIKRLCIIESVFEGEGSATLMSANYPEGFP EDELMTWQFVVPAHLRASVSFLNFNLSNCERKEERVEYYIPGSTTNPEVFKLEDKQPGN MAGNFNLSLQGCDQDAQSPGILRLQFQVLVQHPQNESNKIYVVDLSNERAMSLTIEPRP VKQSRKFVPGCFVCLESRTCSSNLTLTSGSKHKISFLCDDLTRLWMNVEKTIS corresponding to amino acids 1-416 of Q9H5V8 (SEQ ID NO: 989), which also corresponds to amino acids 1-416 of M77904_P4 (SEQ ID NO: 253), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence TPLNQCICPWPWIALLSPPCLSGVPWVGCKSYQKGPSGRARWLTPVIPALWEAKAGGS LEVRSSRPAWPTW corresponding to amino acids 417-487 of M77904_P4 (SEQ ID NO: 253), wherein said first and second amino acid sequences are contiguous and in a sequential order.

2. An isolated polypeptide encoding for a tail of M77904_P4 (SEQ ID NO: 253), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence TPLNQCICPWPWIALLSPPCLSGVPWVGCKSYQKGPSGRARWLTPVIPALWEAKAGGS LEVRSSRPAWPTW in M77904_P4 (SEQ ID NO: 253).

Comparison report between M77904_P4 (SEQ ID NO: 253) and Q96QU7 (SEQ ID NO: 988):

1. An isolated chimeric polypeptide encoding for M77904_P4 (SEQ ID NO: 253), comprising a first amino acid sequence being at least 90% homologous to MAGLNCGVSIALLGVLLLGAARLPRGAEAFEIALPRESNITVLIKLGTPTLLAKPCYIVIS KRHITMLSIKSGERIVFTFSCQSPENHFVIEIQKNIDCMSGPCPFGEVQLQPSTSLLPTLNR TFIWDVKAHKSIGLELQFSIPRLRQIGPGESCPDGVTHSISGRIDATVVRIGTFCSNGTVSR IKMQEGVKMALHLPWFHPRNVSGFSIANRSSIKRLCIIESVFEGEGSATLMSANYPEGFP EDELMTWQFVVPAHLRASVSFLNFNLSNCERKEERVEYYIPGSTTNPEVFKLEDKQPGN MAGNFNLSLQGCDQDAQSPGILRLQFQVLVQHPQNESNKIYVVDLSNERAMSLTIEPRP VKQSRKFVPGCFVCLESRTCSSNLTLTSGSKHKISFLCDDLTRLWMNVEKTIS corresponding to amino acids 1-416 of Q96QU7 (SEQ ID NO: 988), which also corresponds to amino acids 1-416 of M77904_P4 (SEQ ID NO: 253), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence TPLNQCICPWPWIALLSPPCLSGVPWVGCKSYQKGPSGRARWLTPVIPALWEAKAGGS LEVRSSRPAWPTW corresponding to amino acids 417-487 of M77904_P4 (SEQ ID NO: 253), wherein said first and second amino acid sequences are contiguous and in a sequential order.

2. An isolated polypeptide encoding for a tail of M77904_P4 (SEQ ID NO: 253), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence TPLNQCICPWPWIALLSPPCLSGVPWVGCKSYQKGPSGRARWLTPVIPALWEAKAGGS LEVRSSRPAWPTW in M77904_P4 (SEQ ID NO: 253).

The location of the variant protein was determined according to results from a number of different software programs and analyses, including analyses from SignalP and other specialized programs. The variant protein is believed to be located as follows with regard to the cell: secreted. The protein localization is believed to be secreted because both signal-peptide prediction programs predict that this protein has a signal peptide, and neither trans-membrane region prediction program predicts that this protein has a trans-membrane region.

Variant protein M77904_P4 (SEQ ID NO: 253) also has the following non-silent SNPs (Single Nucleotide Polymorphisms) as listed in Table 8, (given according to their position(s) on the amino acid sequence, with the alternative amino acid(s) listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein M77904_P4 (SEQ ID NO: 253) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 8 Amino acid mutations SNP position(s) on amino acid Previously sequence Alternative amino acid(s) known SNP? 329 Q -> R No

Variant protein M77904_P4 (SEQ ID NO: 253) is encoded by the following transcript(s): M77904_T8 (SEQ ID NO: 229), for which the sequence(s) is/are given at the end of the application. The coding portion of transcript M77904_T8 (SEQ ID NO: 229) is shown in bold; this coding portion starts at position 137 and ends at position 1597. The transcript also has the following SNPs as listed in Table 9 (given according to their position on the nucleotide sequence, with the alternative nucleic acid listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein M77904_P4 (SEQ ID NO: 253) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 9 Nucleic acid SNPs SNP position on nucleotide Previously sequence Alternative nucleic acid known SNP? 54 G -> No 59 G -> No 131 G -> C Yes 658 C -> T No 682 T -> C No 1054 G -> A Yes 1122 A -> G No

Variant protein M77904_P5 (SEQ ID NO: 254) according to the present invention has an amino acid sequence as given at the end of the application; it is encoded by transcript(s) M77904_T9 (SEQ ID NO: 230). One or more alignments to one or more previously published protein sequences are given at the end of the application. A brief description of the relationship of the variant protein according to the present invention to each such aligned protein is as follows:

Comparison report between M77904_P5 (SEQ ID NO: 254) and Q96QU7 (SEQ ID NO: 988):

1. An isolated chimeric polypeptide encoding for M77904_P5 (SEQ ID NO: 254), comprising a first amino acid sequence being at least 90% homologous to MIIQEQRTRAEEIFSLDEDVLPKPSFHHHSFWVNISNCSPTSGKQLDLLFSVTLTPRTVDL TVILIAAVGGGVLLLSALGLIICCVKKKKKKTNKGPAVGIYNGNINTEMPRQPKKFQKG RKDNDSHVYAVIEDTMVYGHLLQDSSGSFLQPEVDTYRPFQGTMGVCPPSPPTICSRAP TAKLATEEPPPRSPPESESEPYTFSHPNNGDVSSKDTDIPLLNTQEPMEPAE corresponding to amino acids 606-836 of Q96QU7 (SEQ ID NO: 988), which also corresponds to amino acids 1-231 of M77904_P5 (SEQ ID NO: 254).

Comparison report between M77904_P5 (SEQ ID NO: 254) and Q9H₈C2 (SEQ ID NO: 990) (SEQ ID NO:990):

1. An isolated chimeric polypeptide encoding for M77904_P5 (SEQ ID NO: 254), comprising a first amino acid sequence being at least 90% homologous to MIIQEQRTRAEEIFSLDEDVLPKPSFHHHSFWVNISNCSPTSGKQLDLLFSVTLTPRTVDL TVILIAAVGGGVLLLSALGLIICCVKKKKKKTNKGPAVGIYNGNINTEMPRQPKKFQKG RKDNDSHVYAVIEDTMVYGHLLQDSSGSFLQPEVDTYRPFQGTMGVCPPSPPTICSRAP TAKLATEEPPPRSPPESESEPYTFSHPNNGDVSSKDTDIPLLNTQEPMEPAE corresponding to amino acids 419-649 of Q9H₈C2 (SEQ ID NO: 990), which also corresponds to amino acids 1-231 of M77904_P5 (SEQ ID NO: 254).

The location of the variant protein was determined according to results from a number of different software programs and analyses, including analyses from SignalP and other specialized programs. The variant protein is believed to be located as follows with regard to the cell: membrane. The protein localization is believed to be membrane because both trans-membrane region prediction programs predicted a trans-membrane region for this protein. In addition both signal-peptide prediction programs predict that this protein is a non-secreted protein.

Variant protein M77904_P5 (SEQ ID NO: 254) also has the following non-silent SNPs (Single Nucleotide Polymorphisms) as listed in Table 10, (given according to their position(s) on the amino acid sequence, with the alternative amino acid(s) listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein M77904_P5 (SEQ ID NO: 254) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 10 Amino acid mutations SNP position(s) on amino acid Alternative sequence amino acid(s) Previously known SNP? 104 G -> D Yes

Variant protein M77904_P5 (SEQ ID NO: 254) is encoded by the following transcript(s): M77904_T9 (SEQ ID NO: 230), for which the sequence(s) is/are given at the end of the application. The coding portion of transcript M77904_T9 (SEQ ID NO: 230) is shown in bold; this coding portion starts at position 1226 and ends at position 1918. The transcript also has the following SNPs as listed in Table 11 (given according to their position on the nucleotide sequence, with the alternative nucleic acid listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein M77904_P5 (SEQ ID NO: 254) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 11 Nucleic acid SNPs SNP position on nucleotide Alternative sequence nucleic acid Previously known SNP? 248 A -> C Yes 318 G -> C Yes 3131 A -> T Yes 3201 G -> A Yes 3271 A -> G Yes 3331 C -> A Yes 3485 G -> A Yes 3984 C -> T Yes 4421 G -> A No 4680 A -> C Yes 4700 A -> G Yes 4791 T -> C Yes 984 A -> G Yes 4861 T -> C Yes 4878 C -> A Yes 4882 G -> A Yes 4949 T -> G Yes 5033 A -> C No 994 C -> T Yes 1456 T -> C No 1536 G -> A Yes 2414 T -> C No 2493 G -> A Yes 2647 T -> G Yes 2836 C -> T Yes

Variant protein M77904_P7 (SEQ ID NO: 255) according to the present invention has an amino acid sequence as given at the end of the application; it is encoded by transcript(s) M77904_T11 (SEQ ID NO: 227). One or more alignments to one or more previously published protein sequences are given at the end of the application. A brief description of the relationship of the variant protein according to the present invention to each such aligned protein is as follows:

Comparison report between M77904_P7 (SEQ ID NO: 255) and Q8WU91 (SEQ ID NO: 987):

1. An isolated chimeric polypeptide encoding for M77904_P7 (SEQ ID NO: 255), comprising a first amino acid sequence being at least 90% homologous to MAGLNCGVSIALLGVLLLGAARLPRGAEAFEIALPRESNITVLIKLGTPTLLAKPCYIVIS KRHITMLSIKSGERIVFTFSCQSPENHFVIEIQKNIIDCMSGPCPFGEVQLQPSTSLLPTLNR TFIWDVKAHKSIGLELQFSIPRLRQIGPGESCPDGVTHSISGRIDATVVRIGTFCSNGTVSR IKMQEGVKMALHLPWFHPRNVSGFSIANRSSIKR corresponding to amino acids 1-219 of Q8WU91 (SEQ ID NO: 987), which also corresponds to amino acids 1-219 of M77904_P7 (SEQ ID NO: 255), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence EKAPPCYLIRLKHTRSSLF (SEQ ID NO: 1137) corresponding to amino acids 220-238 of M77904_P7 (SEQ ID NO: 255), wherein said first and second amino acid sequences are contiguous and in a sequential order.

2. An isolated polypeptide encoding for a tail of M77904_P7 (SEQ ID NO: 255), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence EKAPPCYLIRLKHTRSSLF (SEQ ID NO: 1137) in M77904_P7 (SEQ ID NO: 255).

Comparison report between M77904_P7 (SEQ ID NO: 255) and Q9H5V8 (SEQ ID NO: 989):

1. An isolated chimeric polypeptide encoding for M77904_P7 (SEQ ID NO: 255), comprising a first amino acid sequence being at least 90% homologous to MAGLNCGVSIALLGVLLLGAARLPRGAEAFEIALPRESNITVLIKLGTPTLLAKPCYIVIS KRHITMLSIKSGERIVFTFSCQSPENHFVIEIQKNIDCMSGPCPFGEVQLQPSTSLLPTLNR TFIWDVKAHKSIGLELQFSIPRLRQIGPGESCPDGVTHSISGRIDATVVRIGTFCSNGTVSR IKMQEGVKMALHLPWFHPRNVSGFSIANRSSIKR corresponding to amino acids 1-219 of Q9H5V8 (SEQ ID NO: 989), which also corresponds to amino acids 1-219 of M77904_P7 (SEQ ID NO: 255), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence EKAPPCYLIRLKHTRSSLF (SEQ ID NO: 1137) corresponding to amino acids 220-238 of M77904_P7 (SEQ ID NO: 255), wherein said first and second amino acid sequences are contiguous and in a sequential order.

2. An isolated polypeptide encoding for a tail of M77904_P7 (SEQ ID NO: 255), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence EKAPPCYLIRLKHTRSSLF (SEQ ID NO: 1137) in M77904_P7 (SEQ ID NO: 255).

Comparison report between M77904_P7 (SEQ ID NO: 255) and Q96QU7 (SEQ ID NO: 988):

1. An isolated chimeric polypeptide encoding for M77904_P7 (SEQ ID NO: 255), comprising a first amino acid sequence being at least 90% homologous to MAGLNCGVSIALLGVLLLGAARLPRGAEAFEIALPRESNITVLIKLGTPTLLAKPCYIVIS KRHITMLSIKSGERIVFTFSCQSPENHFVIEIQKNIDCMSGPCPFGEVQLQPSTSLLPTLNR TFIWDVKAHKSIGLELQFSIPRLRQIGPGESCPDGVTHSISGRIDATVVRIGTFCSNGTVSR IKMQEGVKMALHLPWFHPRNVSGFSIANRSSIKR corresponding to amino acids 1-219 of Q96QU7 (SEQ ID NO: 988), which also corresponds to amino acids 1-219 of M77904_P7 (SEQ ID NO: 255), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence EKAPPCYLIRLKHTRSSLF (SEQ ID NO: 1137) corresponding to amino acids 220-238 of M77904_P7 (SEQ ID NO: 255), wherein said first and second amino acid sequences are contiguous and in a sequential order.

2. An isolated polypeptide encoding for a tail of M77904_P7 (SEQ ID NO: 255), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence EKAPPCYLIRLKHTRSSLF (SEQ ID NO: 1137) in M77904_P7 (SEQ ID NO: 255).

The location of the variant protein was determined according to results from a number of different software programs and analyses, including analyses from SignalP and other specialized programs. The variant protein is believed to be located as follows with regard to the cell: secreted. The protein localization is believed to be secreted because both signal-peptide prediction programs predict that this protein has a signal peptide, and neither trans-membrane region prediction program predicts that this protein has a trans-membrane region.

Variant protein M77904_P7 (SEQ ID NO: 255) is encoded by the following transcript(s): M77904_T11 (SEQ ID NO: 227), for which the sequence(s) is/are given at the end of the application. The coding portion of transcript M77904_T11 (SEQ ID NO: 227) is shown in bold; this coding portion starts at position 137 and ends at position 850. The transcript also has the following SNPs as listed in Table 12 (given according to their position on the nucleotide sequence, with the alternative nucleic acid listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein M77904_P7 (SEQ ID NO: 255) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 12 Nucleic acid SNPs SNP position on nucleotide Alternative sequence nucleic acid Previously known SNP? 54 G -> No 59 G -> No 2361 A -> G No 131 G -> C Yes 658 C -> T No 682 T -> C No 943 C -> T Yes 1667 G -> A No 1700 G -> A No 1807 T -> C Yes 2293 G -> A Yes

As noted above, cluster M77904 features 21 segment(s), which were listed in Table 2 above and for which the sequence(s) are given at the end of the application. These segment(s) are portions of nucleic acid sequence(s) which are described herein separately because they are of particular interest. A description of each segment according to the present invention is now provided.

Segment cluster M77904_node_(—)0 (SEQ ID NO: 231) according to the present invention is supported by 32 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): M77904_T11 (SEQ ID NO: 227) and M77904_T8 (SEQ ID NO: 229). Table 13 below describes the starting and ending position of this segment on each transcript.

TABLE 13 Segment location on transcripts Segment Segment Transcript starting ending name position position M77904_T11 1 218 (SEQ ID NO:227) M77904_T8 1 218 (SEQ ID NO:229)

Segment cluster M77904_node_(—)11 (SEQ ID NO: 232) according to the present invention is supported by 37 libraries. The number of libraries was determined as previously described.

This segment can be found in the following transcript(s): M77904_T3 (SEQ ID NO: 228) and M77904_T8 (SEQ ID NO: 229). Table 14 below describes the starting and ending position of this segment on each transcript.

TABLE 14 Segment location on transcripts Segment Segment Transcript starting ending name position position M77904_T3 1064 1285 (SEQ ID NO:228) M77904_T8 1161 1382 (SEQ ID NO:229)

Segment cluster M77904_node_(—)12 (SEQ ID NO: 233) according to the present invention is supported by 4 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): M77904_T8 (SEQ ID NO: 229). Table 15 below describes the starting and ending position of this segment on each transcript.

TABLE 15 Segment location on transcripts Segment Segment Transcript starting ending name position position M77904_T8 1383 1785 (SEQ ID NO:229)

Segment cluster M77904_node_(—)14 (SEQ ID NO: 234) according to the present invention is supported by 5 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): M77904_T9 (SEQ ID NO: 230). Table 16 below describes the starting and ending position of this segment on each transcript.

TABLE 16 Segment location on transcripts Segment Segment Transcript starting ending name position position M77904_T9 1 656 (SEQ ID NO:230)

Segment cluster M77904_node_(—)15 (SEQ ID NO: 235) according to the present invention is supported by 44 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): M77904_T3 (SEQ ID NO: 228) and M77904_T9 (SEQ ID NO: 230). Table 17 below describes the starting and ending position of this segment on each transcript.

TABLE 17 Segment location on transcripts Segment Segment Transcript starting ending name position position M77904_T3 1286 1666 (SEQ ID NO:228) M77904_T9 657 1037 (SEQ ID NO:230)

Segment cluster M77904_node_(—)17 (SEQ ID NO: 236) according to the present invention is supported by 48 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): M77904_T3 (SEQ ID NO: 228) and M77904_T9 (SEQ ID NO: 230). Table 18 below describes the starting and ending position of this segment on each transcript.

TABLE 18 Segment location on transcripts Segment Segment Transcript starting ending name position position M77904_T3 1667 2032 (SEQ ID NO:228) M77904_T9 1038 1403 (SEQ ID NO:230)

Segment cluster M77904_node_(—)2 (SEQ ID NO: 237) according to the present invention is supported by 1 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): M77904_T3 (SEQ ID NO: 228). Table 19 below describes the starting and ending position of this segment on each transcript.

TABLE 19 Segment location on transcripts Segment Segment Transcript starting ending name position position M77904_T3 1 121 (SEQ ID NO:228)

Segment cluster M77904_node_(—)21 (SEQ ID NO: 238) according to the present invention is supported by 54 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): M77904_T3 (SEQ ID NO: 228) and M77904_T9 (SEQ ID NO: 230). Table 20 below describes the starting and ending position of this segment on each transcript.

TABLE 20 Segment location on transcripts Segment Segment Transcript starting ending name position position M77904_T3 2121 4095 (SEQ ID NO:228) M77904_T9 1492 3466 (SEQ ID NO:230)

Segment cluster M77904_node_(—)23 (SEQ ID NO: 239) according to the present invention is supported by 24 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): M77904_T3 (SEQ ID NO: 228) and M77904_T9 (SEQ ID NO: 230). Table 21 below describes the starting and ending position of this segment on each transcript.

TABLE 21 Segment location on transcripts Segment Segment Transcript starting ending name position position M77904_T3 4106 4375 (SEQ ID NO:228) M77904_T9 3477 3746 (SEQ ID NO:230)

Segment cluster M77904_node_(—)24 (SEQ ID NO: 240) according to the present invention is supported by 48 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): M77904_T3 (SEQ ID NO: 228) and M77904_T9 (SEQ ID NO: 230). Table 22 below describes the starting and ending position of this segment on each transcript.

TABLE 22 Segment location on transcripts Segment Segment Transcript starting ending name position position M77904_T3 4376 4785 (SEQ ID NO:228) M77904_T9 3747 4156 (SEQ ID NO:230)

Segment cluster M77904_node_(—)27 (SEQ ID NO: 241) according to the present invention is supported by 81 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): M77904_T3 (SEQ ID NO: 228) and M77904_T9 (SEQ ID NO: 230). Table 23 below describes the starting and ending position of this segment on each transcript.

TABLE 23 Segment location on transcripts Segment Segment Transcript starting ending name position position M77904_T3 4994 5482 (SEQ ID NO:228) M77904_T9 4365 4853 (SEQ ID NO:230)

Segment cluster M77904_node_(—)28 (SEQ ID NO: 242) according to the present invention is supported by 55 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): M77904_T3 (SEQ ID NO: 228) and M77904_T9 (SEQ ID NO: 230). Table 24 below describes the starting and ending position of this segment on each transcript.

TABLE 24 Segment location on transcripts Segment Segment Transcript starting ending name position position M77904_T3 5483 5914 (SEQ ID NO:228) M77904_T9 4854 5285 (SEQ ID NO:230)

Segment cluster M77904_node_(—)4 (SEQ ID NO: 243) according to the present invention is supported by 35 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): M77904_T11 (SEQ ID NO: 227), M77904_T3 (SEQ ID NO: 228) and M77904_T8 (SEQ ID NO: 229). Table 25 below describes the starting and ending position of this segment on each transcript.

TABLE 25 Segment location on transcripts Segment Segment Transcript starting ending name position position M77904_T11 219 428 (SEQ ID NO:227) M77904_T3 122 331 (SEQ ID NO:228) M77904_T8 219 428 (SEQ ID NO:229)

Segment cluster M77904_node_(—)6 (SEQ ID NO: 244) according to the present invention is supported by 44 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): M77904_T11 (SEQ ID NO: 227), M77904_T3 (SEQ ID NO: 228) and M77904_T8 (SEQ ID NO: 229). Table 26 below describes the starting and ending position of this segment on each transcript.

TABLE 26 Segment location on transcripts Segment Segment Transcript starting ending name position position M77904_T11 429 791 (SEQ ID NO:227) M77904_T3 332 694 (SEQ ID NO:228) M77904_T8 429 791 (SEQ ID NO:229)

Segment cluster M77904_node_(—)7 (SEQ ID NO: 245) according to the present invention is supported by 4 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): M77904_T11 (SEQ ID NO: 227). Table 27 below describes the starting and ending position of this segment on each transcript.

TABLE 27 Segment location on transcripts Segment Segment Transcript starting ending name position position M77904_T11 792 2030 (SEQ ID NO:227)

Segment cluster M77904_node_(—)8 (SEQ ID NO: 246) according to the present invention is supported by 50 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): M77904_T11 (SEQ ID NO: 227), M77904_T3 (SEQ ID NO: 228) and M77904_T8 (SEQ ID NO: 229). Table 28 below describes the starting and ending position of this segment on each transcript.

TABLE 28 Segment location on transcripts Segment Segment Transcript starting ending name position position M77904_T11 2031 2399 (SEQ ID NO:227) M77904_T3 695 1063 (SEQ ID NO:228) M77904_T8 792 1160 (SEQ ID NO:229)

Segment cluster M77904_node_(—)9 (SEQ ID NO: 247) according to the present invention is supported by 11 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): M77904_T11 (SEQ ID NO: 227). Table 29 below describes the starting and ending position of this segment on each transcript.

TABLE 29 Segment location on transcripts Segment Segment Transcript starting ending name position position M77904_T11 2400 2658 (SEQ ID NO:227)

According to an optional embodiment of the present invention, short segments related to the above cluster are also provided. These segments are up to about 120 bp in length, and so are included in a separate description.

Segment cluster M77904_node_(—)19 (SEQ ID NO: 248) according to the present invention is supported by 42 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): M77904_T3 (SEQ ID NO: 228) and M77904_T9 (SEQ ID NO: 230). Table 30 below describes the starting and ending position of this segment on each transcript.

TABLE 30 Segment location on transcripts Segment Segment Transcript starting ending name position position M77904_T3 2033 2120 (SEQ ID NO:228) M77904_T9 1404 1491 (SEQ ID NO:230)

Segment cluster M77904_node_(—)22 (SEQ ID NO: 249) according to the present invention can be found in the following transcript(s): M77904_T3 (SEQ ID NO: 228) and M77904_T9 (SEQ ID NO: 230). Table 31 below describes the starting and ending position of this segment on each transcript.

TABLE 31 Segment location on transcripts Segment Segment Transcript starting ending name position position M77904_T3 4096 4105 (SEQ ID NO:228) M77904_T9 3467 3476 (SEQ ID NO:230)

Segment cluster M77904_node_(—)25 (SEQ ID NO: 250) according to the present invention is supported by 40 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): M77904_T3 (SEQ ID NO: 228) and M77904_T9 (SEQ ID NO: 230). Table 32 below describes the starting and ending position of this segment on each transcript.

TABLE 32 Segment location on transcripts Segment Segment Transcript starting ending name position position M77904_T3 4786 4896 (SEQ ID NO:228) M77904_T9 4157 4267 (SEQ ID NO:230)

Segment cluster M77904_node_(—)26 (SEQ ID NO: 251) according to the present invention is supported by 39 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): M77904_T3 (SEQ ID NO: 228) and M77904_T9 (SEQ ID NO: 230). Table 33 below describes the starting and ending position of this segment on each transcript.

TABLE 33 Segment location on transcripts Segment Segment Transcript starting ending name position position M77904_T3 4897 4993 (SEQ ID NO:228) M77904_T9 4268 4364 (SEQ ID NO:230)

Microarray (chip) data is also available for this gene as follows. As described above with regard to the cluster itself, various oligonucleotides were tested for being differentially expressed in various disease conditions, particularly cancer. The following oligonucleotide was found to hit this segment (with regard to ovarian cancer), shown in Table 33.

TABLE 33 Oligonucleotide related to this gene Oligonucleotide Overexpressed Chip name in cancers reference M77904_0_8_0 Ovarian cancer Ovary (SEQ ID NO:1016) Variant protein alignment to the previously known protein: Sequence name: /tmp/c2Fe8npYgJ/QPDZHH46X1:Q8WU91 (SEQ ID NO: 987) Sequence documentation:

Alignment of: M77904_P2 (SEQ ID NO: 252) x Q8WU91 (SEQ ID NO: 987) ..

Alignment segment 1/1:

Quality: 2730.00 Escore: 0 Matching length: 275 Total length: 275 Matching Percent Similarity: 100.00 Matching Percent Identity: 100.00 Total Percent Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0

Alignment:

Sequence name: /tmp/c2Fe8npYgJ/QPDZHH46X1:Q96QU7 (SEQ ID NO: 988) Sequence documentation:

Alignment of: M77904_P2 (SEQ ID NO: 252) x Q96QU7 (SEQ ID NO: 988) ..

Alignment segment 1/1:

Quality: 7633.00 Escore: 0 Matching length: 770 Total length: 770 Matching Percent Similarity: 100.00 Matching Percent Identity: 100.00 Total Percent Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0

Alignment:

Sequence name: /tmp/4AUsKD5TnV/TBRg9DoebW:Q8WU91 (SEQ ID NO: 987) Sequence documentation:

Alignment of: M77904_P4 (SEQ ID NO: 253) x Q8WU91 (SEQ ID NO: 987) ..

Alignment segment 1/1:

Quality: 3341.00 Escore: 0 Matching length: 341 Total length: 341 Matching Percent Similarity: 100.00 Matching Percent Identity: 100.00 Total Percent Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0

Alignment:

Sequence name: /tmp/4AUsKD5TnV/TBRg9DoebW:Q9H5V8 (SEQ ID NO: 989) Sequence documentation:

Alignment of: M77904_P4 (SEQ ID NO: 253) x Q9H5V8 (SEQ ID NO: 989) ..

Alignment segment 1/1:

Quality: 4081.00 Escore: 0 Matching length: 416 Total length: 416 Matching Percent Similarity: 100.00 Matching Percent Identity: 100.00 Total Percent Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0

Alignment:

Sequence name: /tmp/4AUsKD5TnV/TBRg9DoebW:Q96QU7 (SEQ ID NO: 988) Sequence documentation:

Alignment of: M77904_P4 (SEQ ID NO: 253) x Q96QU7 (SEQ ID NO: 988) ..

Alignment segment 1/1:

Quality: 4081.00 Escore: 0 Matching length: 416 Total length: 416 Matching Percent Similarity: 100.00 Matching Percent Identity: 100.00 Total Percent Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0

Alignment:

Sequence name: /tmp/IChL9mLIus/pmgyBTHuqO:Q96QU7 (SEQ ID NO: 988) Sequence documentation:

Alignment of: M77904_P5 (SEQ ID NO: 254) x Q96QU7 (SEQ ID NO: 988) ..

Alignment segment 1/1:

Quality: 2285.00 Escore: 0 Matching length: 231 Total length: 231 Matching Percent Similarity: 100.00 Matching Percent Identity: 100.00 Total Percent Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0

Alignment:

Sequence name: /tmp/IChL9nLIus/pmgyBTHuqO:Q9H₈C2 (SEQ ID NO: 990) Sequence documentation:

Alignment of: M77904_P5 (SEQ ID NO: 254) x Q9H₈C2 (SEQ ID NO: 990) ..

Alignment segment 1/1:

Quality: 2285.00 Escore: 0 Matching length: 231 Total length: 231 Matching Percent Similarity: 100.00 Matching Percent Identity: 100.00 Total Percent Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0

Alignment:

Sequence name: /tmp/sQqi6hWOGJ/KjbKmDd574:Q8WU91 (SEQ ID NO: 987) Sequence documentation:

Alignment of: M77904_P7 (SEQ ID NO: 255) x Q8WU91 (SEQ ID NO: 987) ..

Alignment segment 1/1:

Quality: 2124.00 Escore: 0 Matching length: 219 Total length: 219 Matching Percent Similarity: 100.00 Matching Percent Identity: 100.00 Total Percent Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0

Alignment:

Sequence name: /tmp/sQqi6hWOGJ/KjbKmDd574:Q9H5V8 (SEQ ID NO: 989) Sequence documentation:

Alignment of: M77904_P7 (SEQ ID NO: 255) x Q9H5V8 (SEQ ID NO: 989) ..

Alignment segment 1/1:

Quality: 2124.00 Escore: 0 Matching length: 219 Total length: 219 Matching Percent Similarity: 100.00 Matching Percent Identity: 100.00 Total Percent Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0

Alignment:

Sequence name: /tmp/sQqi6hWOGJ/KjbKmDd574:Q96QU7 (SEQ ID NO: 988) Sequence documentation:

Alignment of: M77904_P7 (SEQ ID NO: 255) x Q96QU7 (SEQ ID NO: 988) ..

Alignment segment 1/1:

Quality: 2124.00 Escore: 0 Matching length: 219 Total length: 219 Matching Percent Similarity: 100.00 Matching Percent Identity: 100.00 Total Percent Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0

Alignment:

Description for Cluster Z25299

Cluster Z25299 features 5 transcript(s) and 11 segment(s) of interest, the names for which are given in Tables 1 and 2, respectively, the sequences themselves are given at the end of the application. The selected protein variants are given in table 3.

TABLE 1 Transcripts of interest Transcript Name SEQ ID NO: Z25299_PEA_2_T1 256 Z25299_PEA_2_T2 257 Z25299_PEA_2_T3 258 Z25299_PEA_2_T6 259 Z25299_PEA_2_T9 260

TABLE 2 Segments of interest Segment Name SEQ ID NO: Z25299_PEA_2_node_20 261 Z25299_PEA_2_node_21 262 Z25299_PEA_2_node_23 263 Z25299_PEA_2_node_24 264 Z25299_PEA_2_node_8 265 Z25299_PEA_2_node_12 266 Z25299_PEA_2_node_13 267 Z25299_PEA_2_node_14 268 Z25299_PEA_2_node_17 269 Z25299_PEA_2_node_18 270 Z25299_PEA_2_node_19 271

TABLE 3 Proteins of interest Protein Name SEQ ID NO: Z25299_PEA_2_P2 273 Z25299_PEA_2_P3 274 Z25299_PEA_2_P7 275 Z25299_PEA_2_P10 276

These sequences are variants of the known protein Antileukoproteinase 1 precursor (SwissProt accession identifier ALK1_HUMAN; known also according to the synonyms ALP; HUSI-1; Seminal proteinase inhibitor; Secretory leukocyte protease inhibitor; BLPI; Mucus proteinase inhibitor; MPI; WAP four-disulfide core domain protein 4; Protease inhibitor WAP4), SEQ ID NO: 272, referred to herein as the previously known protein.

Protein Antileukoproteinase 1 precursor is known or believed to have the following function(s): Acid-stable proteinase inhibitor with strong affinities for trypsin, chymotrypsin, elastase, and cathepsin G. May prevent elastase-mediated damage to oral and possibly other mucosal tissues. The sequence for protein Antileukoproteinase 1 precursor is given at the end of the application, as “Antileukoproteinase 1 precursor amino acid sequence”. Protein Antileukoproteinase 1 precursor localization is believed to be Secreted.

It has been investigated for clinical/therapeutic use in humans, for example as a target for an antibody or small molecule, and/or as a direct therapeutic; available information related to these investigations is as follows. Potential pharmaceutically related or therapeutically related activity or activities of the previously known protein are as follows: Elastase inhibitor; Tryptase inhibitor. A therapeutic role for a protein represented by the cluster has been predicted. The cluster was assigned this field because there was information in the drug database or the public databases (e.g., described herein above) that this protein, or part thereof, is used or can be used for a potential therapeutic indication: Anti-inflammatory; Antiasthma.

The following GO Annotation(s) apply to the previously known protein. The following annotation(s) were found: proteinase inhibitor; serine protease inhibitor, which are annotation(s) related to Molecular Function.

The GO assignment relies on information from one or more of the SwissProt/TremBl Protein knowledgebase, available from <http://www.expasy.ch/sprot/>; or Locuslink, available from <http://www.ncbi.nlm.nih.gov/projects/LocusLink/>.

Cluster Z25299 can be used as a diagnostic marker according to overexpression of transcripts of this cluster in cancer. Expression of such transcripts in normal tissues is also given according to the previously described methods. The term “number” in the left hand column of the table and the numbers on the y-axis of FIG. 25 refer to weighted expression of ESTs in each category, as “parts per million” (ratio of the expression of ESTs for a particular cluster to the expression of all ESTs in that category, according to parts per million).

Overall, the following results were obtained as shown with regard to the histograms in FIG. 25 and Table 4. This cluster is overexpressed (at least at a minimum level) in the following pathological conditions: brain malignant tumors, a mixture of malignant tumors from different tissues and ovarian carcinoma.

TABLE 4 Normal tissue distribution Name of Tissue Number bladder 82 bone 6 brain 0 colon 37 epithelial 145 general 73 head and neck 638 kidney 26 liver 68 lung 465 breast 52 ovary 0 pancreas 20 prostate 36 skin 215 stomach 219 uterus 113

TABLE 5 P values and ratios for expression in cancerous tissue Name of Tissue P1 P2 SP1 R3 SP2 R4 bladder 8.2e−01 8.5e−01 9.2e−01 0.6 9.7e−01 0.5 bone 5.5e−01 7.3e−01 4.0e−01 2.1 4.9e−01 1.5 brain 8.8e−02 1.5e−01 2.3e−03 7.7 1.2e−02 4.8 colon 3.3e−01 2.8e−01 4.2e−01 1.6 4.2e−01 1.5 epithelial 2.5e−01 7.6e−01 3.8e−01 1.0 1 0.6 general 6.4e−03 2.5e−01 1.7e−06 1.6 5.2e−01 0.9 head and neck 3.6e−01 5.9e−01 7.6e−01 0.6 1 0.3 kidney 7.4e−01 8.4e−01 2.1e−01 2.1 4.2e−01 1.4 liver 4.1e−01 9.1e−01 4.2e−02 3.2 6.4e−01 0.8 lung 7.6e−01 8.3e−01 9.8e−01 0.5 1 0.3 breast 5.0e−01 5.5e−01 9.8e−02 1.6 3.4e−01 1.1 ovary 3.7e−02 3.0e−02 6.9e−03 6.1 4.9e−03 5.6 pancreas 3.8e−01 3.6e−01 3.6e−01 1.7 3.9e−01 1.5 prostate 9.1e−01 9.2e−01 8.9e−01 0.5 9.4e−01 0.5 skin 6.0e−01 8.1e−01 9.3e−01 0.4 1 0.1 stomach 3.0e−01 8.1e−01 9.1e−01 0.6 1 0.3 uterus 1.6e−01 1.3e−01 3.2e−02 1.6 3.0e−01 1.1

As noted above, cluster Z25299 features 5 transcript(s), which were listed in Table 1 above. These transcript(s) encode for protein(s) which are variant(s) of protein Antileukoproteinase 1 precursor. A description of each variant protein according to the present invention is now provided.

Variant protein Z25299_PEA_(—)2_P2 (SEQ ID NO: 273) according to the present invention has an amino acid sequence as given at the end of the application; it is encoded by transcript(s) Z25299_PEA_(—)2_T1 (SEQ ID NO: 256). An alignment is given to the known protein (Antileukoproteinase 1 precursor) at the end of the application. One or more alignments to one or more previously published protein sequences are given at the end of the application. A brief description of the relationship of the variant protein according to the present invention to each such aligned protein is as follows:

Comparison report between Z25299_PEA_(—)2_P2 (SEQ ID NO: 273) and ALK1_HUMAN:

1. An isolated chimeric polypeptide encoding for Z25299_PEA_(—)2_P2 (SEQ ID NO: 273), comprising a first amino acid sequence being at least 90% homologous to MKSSGLFPFLVLLALGTLAPWAVEGSGKSFKAGVCPPKKSAQCLRYKKPECQSDWQCP GKKRCCPDTCGIKCLDPVDTPNPTRRKPGKCPVTYGQCLMLNPPNFCEMDGQCKRDLK CCMGMCGKSCVSPVK corresponding to amino acids 1-131 of ALK1_HUMAN, which also corresponds to amino acids 1-131 of Z25299_PEA_(—)2_P2 (SEQ ID NO: 273), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence GKQGMRAH (SEQ ID NO: 1138) corresponding to amino acids 132-139 of Z25299_PEA_(—)2_P2 (SEQ ID NO: 273), wherein said first and second amino acid sequences are contiguous and in a sequential order.

2. An isolated polypeptide encoding for a tail of Z25299_PEA_(—)2_P2 (SEQ ID NO: 273), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence GKQGMRAH (SEQ ID NO: 1138) in Z25299_PEA_(—)2_P2 (SEQ ID NO: 273).

The location of the variant protein was determined according to results from a number of different software programs and analyses, including analyses from SignalP and other specialized programs. The variant protein is believed to be located as follows with regard to the cell: secreted. The protein localization is believed to be secreted because both signal-peptide prediction programs predict that this protein has a signal peptide, and neither trans-membrane region prediction program predicts that this protein has a trans-membrane region.

Variant protein Z25299_PEA_(—)2_P2 (SEQ ID NO: 273) also has the following non-silent SNPs (Single Nucleotide Polymorphisms) as listed in Table 6, (given according to their position(s) on the amino acid sequence, with the alternative amino acid(s) listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein Z25299_PEA_(—)2_P2 (SEQ ID NO: 273) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 6 Amino acid mutations SNP position(s) on amino acid Alternative sequence amino acid(s) Previously known SNP? 136 M -> T Yes 20 P -> No 43 C -> R No 48 K -> N No 83 R -> K No 84 R -> W No

Variant protein Z25299_PEA_(—)2_P2 (SEQ ID NO: 273) is encoded by the following transcript(s): Z25299_PEA_(—)2_T1 (SEQ ID NO: 256), for which the sequence(s) is/are given at the end of the application. The coding portion of transcript Z25299_PEA_(—)2_T1 (SEQ ID NO: 256) is shown in bold; this coding portion starts at position 124 and ends at position 540. The transcript also has the following SNPs as listed in Table 7 (given according to their position on the nucleotide sequence, with the alternative nucleic acid listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein Z25299_PEA_(—)2_P2 (SEQ ID NO: 273) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 7 Nucleic acid SNPs SNP position on nucleotide Alternative sequence nucleic acid Previously known SNP? 122 C -> T No 123 C -> T No 530 T -> C Yes 989 C -> T Yes 1127 C -> T Yes 1162 A -> C Yes 1180 A -> C Yes 1183 A -> C Yes 1216 A -> C Yes 1262 G -> A Yes 183 T -> No 250 T -> C No 267 A -> C No 267 A -> G No 339 C -> T Yes 371 G -> A No 373 A -> T No 435 C -> T No

Variant protein Z25299_PEA_(—)2_P3 (SEQ ID NO: 274) according to the present invention has an amino acid sequence as given at the end of the application; it is encoded by transcript(s) Z25299_PEA_(—)2_T2 (SEQ ID NO: 257). An alignment is given to the known protein (Antileukoproteinase 1 precursor) at the end of the application. One or more alignments to one or more previously published protein sequences are given at the end of the application. A brief description of the relationship of the variant protein according to the present invention to each such aligned protein is as follows:

Comparison report between Z25299_PEA_(—)2_P3 (SEQ ID NO: 274) and ALK1_HUMAN:

1. An isolated chimeric polypeptide encoding for Z25299_PEA_(—)2_P3 (SEQ ID NO: 274), comprising a first amino acid sequence being at least 90% homologous to MKSSGLFPFLVLLALGTLAPWAVEGSGKSFKAGVCPPKKSAQCLRYKKPECQSDWQCP GKKRCCPDTCGIKCLDPVDTPNPTRRKPGKCPVTYGQCLMLNPPNFCEMDGQCKRDLK CCMGMCGKSCVSPVK corresponding to amino acids 1-131 of ALK1_HUMAN, which also corresponds to amino acids 1-131 of Z25299_PEA_(—)2_P3 (SEQ ID NO: 274), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence GEKRHHKQLRDQEVDPLEMRRHSAG (SEQ ID NO: 1139) corresponding to amino acids 132-156 of Z25299_PEA_(—)2_P3 (SEQ ID NO: 274), wherein said first and second amino acid sequences are contiguous and in a sequential order.

2. An isolated polypeptide encoding for a tail of Z25299_PEA_(—)2_P3 (SEQ ID NO: 274), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence GEKRHHKQLRDQEVDPLEMRRHSAG (SEQ ID NO: 1139) in Z25299_PEA_(—)2_P3 (SEQ ID NO: 274).

The location of the variant protein was determined according to results from a number of different software programs and analyses, including analyses from SignalP and other specialized programs. The variant protein is believed to be located as follows with regard to the cell: secreted. The protein localization is believed to be secreted because both signal-peptide prediction programs predict that this protein has a signal peptide, and neither trans-membrane region prediction program predicts that this protein has a trans-membrane region.

Variant protein Z25299_PEA_(—)2_P3 (SEQ ID NO: 274) also has the following non-silent SNPs (Single Nucleotide Polymorphisms) as listed in Table 8, (given according to their position(s) on the amino acid sequence, with the alternative amino acid(s) listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein Z25299_PEA_(—)2_P3 (SEQ ID NO: 274) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 8 Amino acid mutations SNP position(s) on amino acid Alternative sequence amino acid(s) Previously known SNP? 20 P -> No 43 C -> R No 48 K -> N No 83 R -> K No 84 R -> W No

Variant protein Z25299_PEA_(—)2_P3 (SEQ ID NO: 274) is encoded by the following transcript(s): Z25299_PEA_(—)2_T2 (SEQ ID NO: 257), for which the sequence(s) is/are given at the end of the application. The coding portion of transcript Z25299_PEA_(—)2_T2 (SEQ ID NO: 257) is shown in bold; this coding portion starts at position 124 and ends at position 591. The transcript also has the following SNPs as listed in Table 9 (given according to their position on the nucleotide sequence, with the alternative nucleic acid listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein Z25299_PEA_(—)2_P3 (SEQ ID NO: 274) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 9 Nucleic acid SNPs SNP position on nucleotide Alternative sequence nucleic acid Previously known SNP? 122 C -> T No 123 C -> T No 183 T -> No 250 T -> C No 267 A -> C No 267 A -> G No 339 C -> T Yes 371 G -> A No 373 A -> T No 435 C -> T No

Variant protein Z25299_PEA_(—)2_P7 (SEQ ID NO: 275) according to the present invention has an amino acid sequence as given at the end of the application; it is encoded by transcript(s) Z25299_PEA_(—)2_T6 (SEQ ID NO: 259). An alignment is given to the known protein (Antileukoproteinase 1 precursor) at the end of the application. One or more alignments to one or more previously published protein sequences are given at the end of the application. A brief description of the relationship of the variant protein according to the present invention to each such aligned protein is as follows:

Comparison report between Z25299_PEA_(—)2_P7 (SEQ ID NO: 275) and ALK1_HUMAN:

1. An isolated chimeric polypeptide encoding for Z25299_PEA_(—)2_P7 (SEQ ID NO: 275), comprising a first amino acid sequence being at least 90% homologous to MKSSGLFPFLVLLALGTLAPWAVEGSGKSFKAGVCPPKKSAQCLRYKKPECQSDWQCP GKKRCCPDTCGIKCLDPVDTPNP corresponding to amino acids 1-81 of ALK1_HUMAN, which also corresponds to amino acids 1-81 of Z25299_PEA_(—)2_P7 (SEQ ID NO: 275), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence RGSLGSAQ (SEQ ID NO: 1140) corresponding to amino acids 82-89 of Z25299_PEA_(—)2_P7 (SEQ ID NO: 275), wherein said first and second amino acid sequences are contiguous and in a sequential order.

2. An isolated polypeptide encoding for a tail of Z25299_PEA_(—)2_P7 (SEQ ID NO: 275), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence RGSLGSAQ (SEQ ID NO: 1140) in Z25299_PEA_(—)2_P7 (SEQ ID NO: 275).

The location of the variant protein was determined according to results from a number of different software programs and analyses, including analyses from SignalP and other specialized programs. The variant protein is believed to be located as follows with regard to the cell: secreted. The protein localization is believed to be secreted because both signal-peptide prediction programs predict that this protein has a signal peptide, and neither trans-membrane region prediction program predicts that this protein has a trans-membrane region.

Variant protein Z25299_PEA_(—)2_P7 (SEQ ID NO: 275) also has the following non-silent SNPs (Single Nucleotide Polymorphisms) as listed in Table 10, (given according to their position(s) on the amino acid sequence, with the alternative amino acid(s) listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein Z25299_PEA_(—)2_P7 (SEQ ID NO: 275) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 10 Amino acid mutations SNP position(s) on amino acid Alternative sequence amino acid(s) Previously known SNP? 20 P -> No 43 C -> R No 48 K -> N No 82 R -> S No

Variant protein Z25299_PEA_(—)2_P7 (SEQ ID NO: 275) is encoded by the following transcript(s): Z25299_PEA_(—)2_T6 (SEQ ID NO: 259), for which the sequence(s) is/are given at the end of the application. The coding portion of transcript Z25299_PEA_(—)2_T6 (SEQ ID NO: 259) is shown in bold; this coding portion starts at position 124 and ends at position 390. The transcript also has the following SNPs as listed in Table 11 (given according to their position on the nucleotide sequence, with the alternative nucleic acid listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein Z25299_PEA_(—)2_P7 (SEQ ID NO: 275) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 11 Nucleic acid SNPs SNP position on nucleotide Alternative sequence nucleic acid Previously known SNP? 122 C -> T No 123 C -> T No 576 A -> C Yes 594 A -> C Yes 597 A -> C Yes 630 A -> C Yes 676 G -> A Yes 183 T -> No 250 T -> C No 267 A -> C No 267 A -> G No 339 C -> T Yes 369 A -> T No 431 C -> T No 541 C -> T Yes

Variant protein Z25299_PEA_(—)2_P10 (SEQ ID NO: 276) according to the present invention has an amino acid sequence as given at the end of the application; it is encoded by transcript(s) Z25299_PEA_(—)2_T9 (SEQ ID NO: 260). An alignment is given to the known protein (Antileukoproteinase 1 precursor) at the end of the application. One or more alignments to one or more previously published protein sequences are given at the end of the application. A brief description of the relationship of the variant protein according to the present invention to each such aligned protein is as follows:

Comparison report between Z25299_PEA_(—)2_P10 (SEQ ID NO: 276) and ALK1_HUMAN:

1. An isolated chimeric polypeptide encoding for Z25299_PEA_(—)2_P10 (SEQ ID NO: 276), comprising a first amino acid sequence being at least 90% homologous to MKSSGLFPFLVLLALGTLAPWAVEGSGKSFKAGVCPPKKSAQCLRYKKPECQSDWQCP GKKRCCPDTCGIKCLDPVDTPNPT corresponding to amino acids 1-82 of ALK1_HUMAN, which also corresponds to amino acids 1-82 of Z25299_PEA_(—)2_P10 (SEQ ID NO: 276).

The location of the variant protein was determined according to results from a number of different software programs and analyses, including analyses from SignalP and other specialized programs. The variant protein is believed to be located as follows with regard to the cell: secreted. The protein localization is believed to be secreted because both signal-peptide prediction programs predict that this protein has a signal peptide, and neither trans-membrane region prediction program predicts that this protein has a trans-membrane region.

Variant protein Z25299_PEA_(—)2_P10 (SEQ ID NO: 276) also has the following non-silent SNPs (Single Nucleotide Polymorphisms) as listed in Table 12, (given according to their position(s) on the amino acid sequence, with the alternative amino acid(s) listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein Z25299_PEA_(—)2_P10 (SEQ ID NO: 276) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 12 Amino acid mutations SNP position(s) on amino acid equence Alternative amino acid(s) Previously known SNP? 20 P -> No 43 C -> R No 48 K -> N No

Variant protein Z25299_PEA_(—)2_P10 (SEQ ID NO: 276) is encoded by the following transcript(s): Z25299_PEA_(—)2_T9 (SEQ ID NO: 260), for which the sequence(s) is/are given at the end of the application. The coding portion of transcript Z25299_PEA_(—)2_T9 (SEQ ID NO: 260) is shown in bold; this coding portion starts at position 124 and ends at position 369. The transcript also has the following SNPs as listed in Table 13 (given according to their position on the nucleotide sequence, with the alternative nucleic acid listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein Z25299_PEA_(—)2_P10 (SEQ ID NO: 276) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 13 Nucleic acid SNPs SNP position on nucleotide sequence Alternative nucleic acid Previously known SNP? 122 C -> T No 123 C -> T No 451 A -> C Yes 484 A -> C Yes 530 G -> A Yes 183 T -> No 250 T -> C No 267 A -> C No 267 A -> G No 339 C -> T Yes 395 C -> T Yes 430 A -> C Yes 448 A -> C Yes

As noted above, cluster Z25299 features 11 segment(s), which were listed in Table 2 above and for which the sequence(s) are given at the end of the application. These segment(s) are portions of nucleic acid sequence(s) which are described herein separately because they are of particular interest. A description of each segment according to the present invention is now provided.

Segment cluster Z25299_PEA_(—)2_node_(—)20 (SEQ ID NO: 261) according to the present invention is supported by 6 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): Z25299_PEA_(—)2_T1 (SEQ ID NO: 256). Table 14 below describes the starting and ending position of this segment on each transcript.

TABLE 14 Segment location on transcripts Segment Segment Transcript name starting position ending position Z25299_PEA_2_T1 518 1099 (SEQ ID NO: 256)

Segment cluster Z25299_PEA_(—)2_node_(—)21 (SEQ ID NO: 262) according to the present invention is supported by 162 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): Z25299_PEA_(—)2_T1 (SEQ ID NO: 256), Z25299_PEA_(—)2_T6 (SEQ ID NO: 259) and Z25299_PEA_(—)2_T9 (SEQ ID NO: 260). Table 15 below describes the starting and ending position of this segment on each transcript.

TABLE 15 Segment location on transcripts Segment Segment Transcript name starting position ending position Z25299_PEA_2_T1 1100 1292 (SEQ ID NO: 256) Z25299_PEA_2_T6 514 706 (SEQ ID NO: 259) Z25299_PEA_2_T9 368 560 (SEQ ID NO: 260)

Segment cluster Z25299_PEA_(—)2_node_(—)23 (SEQ ID NO: 263) according to the present invention is supported by 2 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): Z25299_PEA_(—)2_T2 (SEQ ID NO: 257). Table 16 below describes the starting and ending position of this segment on each transcript.

TABLE 16 Segment location on transcripts Segment Segment Transcript name starting position ending position Z25299_PEA_2_T2 518 707 (SEQ ID NO: 257)

Segment cluster Z25299_PEA_(—)2_node_(—)24 (SEQ ID NO: 264) according to the present invention is supported by 2 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): Z25299_PEA_(—)2_T2 (SEQ ID NO: 257) and Z25299_PEA_(—)2_T3 (SEQ ID NO: 258). Table 17 below describes the starting and ending position of this segment on each transcript.

TABLE 17 Segment location on transcripts Segment Segment Transcript name starting position ending position Z25299_PEA_2_T2 708 886 (SEQ ID NO: 257) Z25299_PEA_2_T3 518 696 (SEQ ID NO: 258)

Segment cluster Z25299_PEA_(—)2_node_(—)8 (SEQ ID NO: 265) according to the present invention is supported by 218 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): Z25299_PEA_(—)2_T1 (SEQ ID NO: 256), Z25299_PEA_(—)2_T2 (SEQ ID NO: 257), Z25299_PEA_(—)2_T3 (SEQ ID NO: 258), Z25299_PEA_(—)2_T6 (SEQ ID NO: 259) and Z25299_PEA_(—)2_T9 (SEQ ID NO: 260). Table 18 below describes the starting and ending position of this segment on each transcript.

TABLE 18 Segment location on transcripts Segment Segment Transcript name starting position ending position Z25299_PEA_2_T1 1 208 (SEQ ID NO: 256) Z25299_PEA_2_T2 1 208 (SEQ ID NO: 257) Z25299_PEA_2_T3 1 208 (SEQ ID NO: 258) Z25299_PEA_2_T6 1 208 (SEQ ID NO: 259) Z25299_PEA_2_T9 1 208 (SEQ ID NO: 260)

Microarray (chip) data is also available for this segment as follows. As described above with regard to the cluster itself, various oligonucleotides were tested for being differentially expressed in various disease conditions, particularly cancer. The following oligonucleotides were found to hit this segment (with regard to ovarian cancer), shown in Table 19.

TABLE 19 Oligonucleotides related to this segment Oligonucleotide Overexpressed in name cancers Chip reference Z25299_0_3_0 ovarian carcinoma OVA (SEQ ID NO: 1017)

According to an optional embodiment of the present invention, short segments related to the above cluster are also provided. These segments are up to about 120 bp in length, and so are included in a separate description.

Segment cluster Z25299_PEA_(—)2_node_(—)12 (SEQ ID NO: 266) according to the present invention is supported by 228 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): Z25299_PEA_(—)2_T1 (SEQ ID NO: 256), Z25299_PEA_(—)2_T2 (SEQ ID NO: 257), Z25299_PEA_(—)2_T3 (SEQ ID NO: 258), Z25299_PEA_(—)2_T6 (SEQ ID NO: 259) and Z25299_PEA_(—)2_T9 (SEQ ID NO: 260). Table 20 below describes the starting and ending position of this segment on each transcript.

TABLE 20 Segment location on transcripts Segment Segment Transcript name starting position ending position Z25299_PEA_2_T1 209 245 (SEQ ID NO: 256) Z25299_PEA_2_T2 209 245 (SEQ ID NO: 257) Z25299_PEA_2_T3 209 245 (SEQ ID NO: 258) Z25299_PEA_2_T6 209 245 (SEQ ID NO: 259) Z25299_PEA_2_T9 209 245 (SEQ ID NO: 260)

Microarray (chip) data is also available for this segment as follows. As described above with regard to the cluster itself, various oligonucleotides were tested for being differentially expressed in various disease conditions, particularly cancer. The following oligonucleotides were found to hit this segment (in relation to ovarian cancer), shown in Table 21.

TABLE 21 Oligonucleotides related to this segment Oligonucleotide Overexpressed in name cancers Chip reference Z25299_0_3_0 ovarian carcinoma OVA (SEQ ID NO: 1017)

Segment cluster Z25299_PEA_(—)2_node_(—)13 (SEQ ID NO: 267) according to the present invention is supported by 246 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): Z25299_PEA_(—)2_T1 (SEQ ID NO: 256), Z25299_PEA_(—)2_T2 (SEQ ID NO: 257), Z25299_PEA_(—)2_T3 (SEQ ID NO: 258), Z25299_PEA_(—)2_T6 (SEQ ID NO: 259) and Z25299_PEA_(—)2_T9 (SEQ ID NO: 260). Table 22 below describes the starting and ending position of this segment on each transcript.

TABLE 22 Segment location on transcripts Segment Segment Transcript name starting position ending position Z25299_PEA_2_T1 246 357 (SEQ ID NO: 256) Z25299_PEA_2_T2 246 357 (SEQ ID NO: 257) Z25299_PEA_2_T3 246 357 (SEQ ID NO: 258) Z25299_PEA_2_T6 246 357 (SEQ ID NO: 259) Z25299_PEA_2_T9 246 357 (SEQ ID NO: 260)

Segment cluster Z25299_PEA_(—)2_node_(—)14 (SEQ ID NO: 268) Z25299_PEA_(—)2_node_(—)14 (SEQ ID NO: 268) (SEQ ID NO: 268) according to the present invention can be found in the following transcript(s): Z25299_PEA_(—)2_T1 (SEQ ID NO: 256), Z25299_PEA_(—)2_T2 (SEQ ID NO: 257), Z25299_PEA_(—)2_T3 (SEQ ID NO: 258), Z25299_PEA_(—)2_T6 (SEQ ID NO: 259) and Z25299_PEA_(—)2_T9 (SEQ ID NO: 260). Table 23 below describes the starting and ending position of this segment on each transcript.

TABLE 23 Segment location on transcripts Segment Segment Transcript name starting position ending position Z25299_PEA_2_T1 358 367 (SEQ ID NO: 256) Z25299_PEA_2_T2 358 367 (SEQ ID NO: 257) Z25299_PEA_2_T3 358 367 (SEQ ID NO: 258) Z25299_PEA_2_T6 358 367 (SEQ ID NO: 259) Z25299_PEA_2_T9 358 367 (SEQ ID NO: 260)

Segment cluster Z25299_PEA_(—)2_node_(—)17 (SEQ ID NO: 269) according to the present invention can be found in the following transcript(s): Z25299_PEA_(—)2_T1 (SEQ ID NO: 256), Z25299_PEA_(—)2_T2 (SEQ ID NO: 257) and Z25299_PEA_(—)2_T3 (SEQ ID NO: 258). Table 24 below describes the starting and ending position of this segment on each transcript.

TABLE 24 Segment location on transcripts Segment Segment Transcript name starting position ending position Z25299_PEA_2_T1 368 371 (SEQ ID NO: 256) Z25299_PEA_2_T2 368 371 (SEQ ID NO: 257) Z25299_PEA_2_T3 368 371 (SEQ ID NO: 258)

Segment cluster Z25299_PEA_(—)2_node_(—)18 (SEQ ID NO: 270) according to the present invention is supported by 221 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): Z25299_PEA_(—)2_T1 (SEQ ID NO: 256), Z25299_PEA_(—)2_T2 (SEQ ID NO: 257), Z25299_PEA_(—)2_T3 (SEQ ID NO: 258) and Z25299_PEA_(—)2_T6 (SEQ ID NO: 259). Table 25 below describes the starting and ending position of this segment on each transcript.

TABLE 25 Segment location on transcripts Segment Segment Transcript name starting position ending position Z25299_PEA_2_T1 372 427 (SEQ ID NO: 256) Z25299_PEA_2_T2 372 427 (SEQ ID NO: 257) Z25299_PEA_2_T3 372 427 (SEQ ID NO: 258) Z25299_PEA_2_T6 368 423 (SEQ ID NO: 259)

Segment cluster Z25299_PEA_(—)2_node_(—)19 (SEQ ID NO: 271) according to the present invention is supported by 197 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): Z25299_PEA_(—)2_T1 (SEQ ID NO: 256), Z25299_PEA_(—)2_T2 (SEQ ID NO: 257), Z25299_PEA_(—)2_T3 (SEQ ID NO: 258) and Z25299_PEA_(—)2_T6 (SEQ ID NO: 259). Table 26 below describes the starting and ending position of this segment on each transcript.

TABLE 26 Segment location on transcripts Segment Segment Transcript name starting position ending position Z25299_PEA_2_T1 428 517 (SEQ ID NO: 256) Z25299_PEA_2_T2 428 517 (SEQ ID NO: 257) Z25299_PEA_2_T3 428 517 (SEQ ID NO: 258) Z25299_PEA_2_T6 424 513 (SEQ ID NO: 259) Variant protein alignment to the previously known protein: Sequence name: /tmp/oXgeQ4MeyL/K6Vqb1MQu2:ALK1_HUMAN Sequence documentation:

Alignment of: Z25299_PEA_(—)2_P2 (SEQ ID NO: 273) x ALK1_HUMAN ..

Alignment segment 1/1:

Quality: 1371.00 Escore: 0 Matching length: 131 Total length: 131 Matching Percent Similarity: 100.00 Matching Percent Identity: 100.00 Total Percent Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0

Alignment:

Sequence name: /tmp/rbf314VLIm/yR4314SbP4:ALK1_HUMAN Sequence documentation:

Alignment of: Z25299_PEA_(—)2_P3 (SEQ ID NO: 274) x ALK1_HUMAN ..

Alignment segment 1/1:

Quality: 1371.00 Escore: 0 Matching length: 131 Total length: 131 Matching Percent Similarity: 100.00 Matching Percent Identity: 100.00 Total Percent Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0

Alignment:

Sequence name: /tmp/KCtSXACZXe/rK4T6LKeRX:ALK1_HUMAN Sequence documentation:

Alignment of: Z25299_PEA_(—)2_P7 (SEQ ID NO: 275) x ALK1_HUMAN ..

Alignment segment 1/1:

Quality: 835.00 Escore: 0 Matching length: 81 Total length: 81 Matching Percent Similarity: 100.00 Matching Percent Identity: 100.00 Total Percent Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0

Alignment:

Sequence name: /tmp/LcBlcAxB6c/NSI9pqfxoU:ALK1_HUMAN Sequence documentation:

Alignment of: Z25299_PEA_(—)2_P10 (SEQ ID NO: 276) x ALK1_HUMAN ..

Alignment segment 1/1:

Quality: 844.00 Escore: 0 Matching length: 82 Total length: 82 Matching Percent Similarity: 100.00 Matching Percent Identity: 100.00 Total Percent Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0

Alignment:

Expression of Secretory Leukocyte Protease Inhibitor Acid-Stable Proteinase Inhibitor Z25299 Transcripts which are Detectable by Amplicon as Depicted in Sequence Name Z25299 junc13-14-21 (SEQ ID NO:993) in Normal and Cancerous Ovary Tissues

Expression of Secretory leukocyte protease inhibitor Acid-stable proteinase inhibitor transcripts detectable by or according to junc13-14-21, Z25299 junc13-14-21 (SEQ ID NO:993) amplicon(s) and Z25299 junc13-14-21F (SEQ ID NO:991) and Z25299 junc13-14-21R (SEQ ID NO:992) primers was measured by real time PCR. In parallel the expression of four housekeeping genes —PBGD (GenBank Accession No. BC019323, (SEQ ID NO:1036); amplicon—PBGD-amplicon, (SEQ ID NO:1039)), HPRT1 (GenBank Accession No. NM_(—)000194, (SEQ ID NO:1040); amplicon—HPRT1-amplicon, (SEQ ID NO:1043)), SDHA (GenBank Accession No. NM_(—)004168, (SEQ ID NO: 1032); amplicon—SDHA-amplicon, (SEQ ID NO:1035)), and GAPDH (GenBank Accession No. BC026907, (SEQ ID NO:1044); GAPDH amplicon, (SEQ ID NO: 1047)) was measured similarly. For each RT sample, the expression of the above amplicon was normalized to the geometric mean of the quantities of the housekeeping genes. The normalized quantity of each RT sample was then divided by the median of the quantities of the normal post-mortem (PM) samples (Sample Nos. 45-48, 71, Table 1, “Tissue samples in testing panel”, above), to obtain a value of fold up-regulation for each sample relative to median of the normal PM samples.

FIG. 26 is a histogram showing over expression of the above-indicated Secretory leukocyte protease inhibitor Acid-stable proteinase inhibitor transcripts in cancerous ovary samples relative to the normal samples. The number and percentage of samples that exhibit at least 5 fold over-expression, out of the total number of samples tested is indicated in the bottom.

As is evident from FIG. 26, the expression of Secretory leukocyte protease inhibitor Acid-stable proteinase inhibitor transcripts detectable by the above amplicon(s) in cancer samples was significantly higher than in the non-cancerous samples (Sample Nos. 45-48, 71, Table 1, “Tissue samples in testing panel”). Notably an over-expression of at least 5 fold was found in 12 out of 42 adenocarcinoma samples.

Statistical analysis was applied to verify the significance of these results, as described below. The P value for the difference in the expression levels of Secretory leukocyte protease inhibitor Acid-stable proteinase inhibitor transcripts detectable by the above amplicon(s) in ovary cancer samples versus the normal tissue samples was determined by T test as 3.0E-04. The above value demonstrates statistical significance of the results.

Primer pairs are also optionally and preferably encompassed within the present invention; for example, for the above experiment, the following primer pair was used as a non-limiting illustrative example only of a suitable primer pair: Z25299 junc13-14-21F (SEQ ID NO:991) forward primer; and Z25299 junc13-14-21R (SEQ ID NO:992) reverse primer.

The present invention also preferably encompasses any amplicon obtained through the use of any suitable primer pair; for example, for the above experiment, the following amplicon was obtained as a non-limiting illustrative example only of a suitable amplicon: Z25299 junc13-14-21 (SEQ ID NO:993).

Z25299 junc13-14-21 Forward primer (SEQ ID NO:991): ACCCCAAACCCAACTTGATTC Z25299junc13-14-21 Reverse primer (SEQ ID NO:992): TCAGTGGTGGAGCCAAGTCTC Z25299 junc13-14-21 Amplicon (SEQ ID NO:993): ACCCCAAACCCAACTTGATTCCTGCCATATGGAGGAGGCTCTGGAGTCCT GCTCTGTGTGGTCCAGGTCCTTTCCACCCTGAGACTTGGCTCCACCACTG A

Expression of Secretory Leukocyte Protease Inhibitor Acid-Stable Proteinase Inhibitor Z25299 Transcripts, which are Detectable by Amplicon as Depicted in Sequence Name Z25299 seg20 (SEQ ID NO:996) in Normal and Cancerous Ovary Tissues

Expression of Secretory leukocyte protease inhibitor Acid-stable proteinase inhibitor transcripts detectable by or according to seg20, Z25299 seg20 amplicon(s) (SEQ ID NO:996) and Z25299 seg20F (SEQ ID NO:994) and Z25299 seg20R (SEQ ID NO:995) primers was measured by real time PCR. In parallel the expression of four housekeeping genes —PBGD (GenBank Accession No. BC019323, (SEQ ID NO:1036); amplicon—PBGD-amplicon, (SEQ ID NO:1039)), HPRT1 (GenBank Accession No. NM_(—)000194, (SEQ ID NO:1040); amplicon—HPRT1-amplicon, (SEQ ID NO:1043)), SDHA (GenBank Accession No. NM_(—)004168, (SEQ ID NO:1032); amplicon—SDHA-amplicon, (SEQ ID NO:1035)), and GAPDH (GenBank Accession No. BC026907, (SEQ ID NO:1044); GAPDH amplicon, (SEQ ID NO:1047)) was measured similarly. For each RT sample, the expression of the above amplicon was normalized to the geometric mean of the quantities of the housekeeping genes. The normalized quantity of each RT sample was then divided by the median of the quantities of the normal post-mortem (PM) samples (Sample Nos. 45-48, 71, Table 1, “Tissue samples in testing panel” above), to obtain a value of fold up-regulation for each sample relative to median of the normal PM samples.

FIG. 27A is a histogram showing over expression of the above-indicated Secretory leukocyte protease inhibitor Acid-stable proteinase transcripts in cancerous ovary samples relative to the normal samples. As is evident from FIG. 27A, the expression of Secretory leukocyte protease inhibitor Acid-stable proteinase transcripts detectable by the above amplicon(s) in cancer samples was significantly higher than in the non-cancerous samples (Sample Nos. 45-48, 71, Table 1, “Tissue samples in testing panel”). Notably an over-expression of at least 10 fold was found in 30 out of 43 adenocarcinoma samples.

Statistical analysis was applied to verify the significance of these results, as described below. The P value for the difference in the expression levels of Secretory leukocyte protease inhibitor Acid-stable proteinase inhibitor transcripts detectable by the above amplicon(s) in ovary cancer samples versus the normal tissue samples was determined by T test as 9.81E-07. Threshold of 10 fold overexpression was found to differentiate between cancer and normal samples with P value of 5E-03 as checked by exact fisher test. The above values demonstrate statistical significance of the results.

Primer pairs are also optionally and preferably encompassed within the present invention; for example, for the above experiment, the following primer pair was used as a non-limiting illustrative example only of a suitable primer pair: Z25299 seg20F forward primer; and Z25299 seg20R reverse primer.

The present invention also preferably encompasses any amplicon obtained through the use of any suitable primer pair; for example, for the above experiment, the following amplicon was obtained as a non-limiting illustrative example only of a suitable amplicon: Z25299 seg20.

Z25299 seg20 Forward primer (SEQ ID NO:994): CTCCTGAACCCTACTCCAAGCA Z25299 seg20 Reverse primer (SEQ ID NO:995): CAGGCGATCCTATGGAAATCC Z25299 seg20 Amplicon (SEQ ID NO:996): CTCCTGAACCCTACTCCAAGCACAGCCTCTGTCTGACTCCCTTGTCCTTC AAGAGAACTGTTCTCCAGGTCTCAGGGCCAGGATTTCCATAGGATCGCCT G

Expression of Secretory Leukocyte Protease Inhibitor (Acid-Stable Proteinase Inhibitor with Strong Affinities for Trypsin, Chymotrypsin, Elastase, and Cathepsin G) Z25299 Transcripts which are Detectable by Amplicon as Depicted in Sequence Name Z25299seg20 (SEQ ID NO:996) in Different Normal Tissues

Expression of Secretory leukocyte protease inhibitor transcripts detectable by or according to Z25299seg20 (SEQ ID NO:996) amplicon(s) and primers: Z25299seg23F (SEQ ID NO:994) Z25299seg20R (SEQ ID NO:995) was measured by real time PCR. In parallel the expression of four housekeeping genes -RPL19 (GenBank Accession No. NM_(—)000981, (SEQ ID NO:1048); RPL19 amplicon, (SEQ ID NO:1051)), TATA box (GenBank Accession No. NM_(—)003194, (SEQ ID NO:1052); TATA amplicon, (SEQ ID NO:1055)), Ubiquitin (GenBank Accession No. BC000449, (SEQ ID NO: 1056); amplicon—Ubiquitin-amplicon, (SEQ ID NO: 1059)) and SDHA (GenBank Accession No. NM_(—)004168, (SEQ ID NO: 1032); amplicon—SDHA-amplicon, (SEQ ID NO:1035)) was measured similarly. For each RT sample, the expression of the above amplicon was normalized to the geometric mean of the quantities of the housekeeping genes. The normalized quantity of each RT sample was then divided by the median of the quantities of the ovary samples (Sample Nos. 18-20, Table 1 above, Tissue samples in testing panel), to obtain a value of relative expression of each sample relative to median of the ovary samples. Primers and amplicon are as above. Results are shown in FIG. 27B.

Expression of Secretory Leukocyte Protease Inhibitor Z25299 Transcripts, which are Detectable by Amplicon as Depicted in Sequence Name Z25299 seg23 (SEQ ID NO:999) in Normal and Cancerous Ovary Tissues

Expression of Secretory leukocyte protease inhibitor Acid-stable proteinase inhibitor transcripts detectable by or according to seg23, Z25299 seg23 (SEQ ID NO:999) amplicon(s) and Z25299 seg23F (SEQ ID NO:997) and Z25299 seg23R (SEQ ID NO:998) primers was measured by real time PCR. In parallel the expression of four housekeeping genes —PBGD (GenBank Accession No. BC019323, (SEQ ID NO: 1036); amplicon—PBGD-amplicon, (SEQ ID NO: 1039)), HPRT1 (GenBank Accession No. NM_(—)000194, (SEQ ID NO: 1040); amplicon—HPRT1-amplicon, (SEQ ID NO:1043)), SDHA (GenBank Accession No. NM_(—)004168, (SEQ ID NO: 1032); amplicon—SDHA-amplicon, (SEQ ID NO: 1035)), and GAPDH (GenBank Accession No. BC026907, (SEQ ID NO:1044); GAPDH amplicon, (SEQ ID NO:1047)) was measured similarly. For each RT sample, the expression of the above amplicon was normalized to the geometric mean of the quantities of the housekeeping genes. The normalized quantity of each RT sample was then divided by the median of the quantities of the normal post-mortem (PM) samples (Sample Nos. 45-48, 71, Table 1, “Tissue samples in testing panel” above), to obtain a value of fold up-regulation for each sample relative to median of the normal PM samples.

FIG. 28A is a histogram showing over expression of the above-indicated Secretory leukocyte protease inhibitor Acid-stable proteinase inhibitor transcripts in cancerous ovary samples relative to the normal samples.

As is evident from FIG. 28A, the expression of Secretory leukocyte protease inhibitor Acid-stable proteinase inhibitor transcripts detectable by the above amplicon(s) in cancer samples was significantly higher than in the non-cancerous samples (Sample Nos. 45-48, 71, Table 1, “Tissue samples in testing panel”). Notably an over-expression of at least 10 fold was found in 31 out of 43 adenocarcinoma samples.

Statistical analysis was applied to verify the significance of these results, as described below. The P value for the difference in the expression levels of Secretory leukocyte protease inhibitor Acid-stable proteinase inhibitor transcripts detectable by the above amplicon(s) in ovary cancer samples versus the normal tissue samples was determined by T test as 2.48E-07. Threshold of 10 fold overexpression was found to differentiate between cancer and normal samples with P value of 3.61E-03 as checked by exact fisher test. The above values demonstrate statistical significance of the results.

Primer pairs are also optionally and preferably encompassed within the present invention; for example, for the above experiment, the following primer pair was used as a non-limiting illustrative example only of a suitable primer pair: Z25299 seg23F forward primer; and Z25299 seg23R reverse primer.

The present invention also preferably encompasses any amplicon obtained through the use of any suitable primer pair; for example, for the above experiment, the following amplicon was obtained as a non-limiting illustrative example only of a suitable amplicon: Z25299 seg23.

Z25299 seg23 Forward primer (SEQ ID NO:997): CAAGCAATTGAGGGACCAGG Z25299 seg23 Reverse primer (SEQ ID NO:998): CAAAAAACATTGTTAATGAGAGAGATGAC Z25299 seg23 Amplicon (SEQ ID NO:999): CAAGCAATTGAGGGACCAGGAAGTGGATCCTCTAGAGATGAGGAGGCATT CTGCTGGATGACTTTTAAAAATGTTTTCTCCAGAGTCATCTCTCTCATTA ACAATGTTTTTTG

Expression of Secretory Leukocyte Protease Inhibitor Z25299 Transcripts which are Detectable by Amplicon as Depicted in Sequence Name Z25299seg23 (SEQ ID NO:999) in Different Normal Tissues

Expression of Secretory leukocyte protease inhibitor transcripts detectable by or according to Z25299seg23 (SEQ ID NO:999) amplicon(s) and primers (as above): Z25299seg23F (SEQ ID NO:997) Z25299seg23R (SEQ ID NO:998) was measured by real time PCR. In parallel the expression of four housekeeping genes -RPL19 (GenBank Accession No. NM_(—)000981, (SEQ ID NO:1048); RPL19 amplicon, (SEQ ID NO:1051)), TATA box (GenBank Accession No. NM_(—)003194, (SEQ ID NO:1052); TATA amplicon, (SEQ ID NO:1055)), Ubiquitin (GenBank Accession No. BC000449, (SEQ ID NO: 1056); amplicon—Ubiquitin-amplicon, (SEQ ID NO:1059)) and SDHA (GenBank Accession No. NM_(—)004168, (SEQ ID NO: 1032); amplicon—SDHA-amplicon, (SEQ ID NO: 1035)) was measured similarly. For each RT sample, the expression of the above amplicon was normalized to the geometric mean of the quantities of the housekeeping genes. The normalized quantity of each RT sample was then divided by the median of the quantities of the ovary samples (Sample Nos. 18-20, Table 1 above, Tissue samples in testing panel), to obtain a value of relative expression of each sample relative to median of the ovary samples. Results are shown in FIG. 28B.

Description for Cluster T39971

Cluster T39971 features 4 transcript(s) and 28 segment(s) of interest, the names for which are given in Tables 1 and 2, respectively, the sequences themselves are given at the end of the application. The selected protein variants are given in table 3.

TABLE 1 Transcripts of interest Transcript Name SEQ ID NO: T39971_T10 570 T39971_T12 571 T39971_116 572 T39971_T5 573

TABLE 2 Segments of interest Segment Name SEQ ID NO: T39971_node_0 574 T39971_node_18 575 T39971_node_21 576 T39971_node_22 577 T39971_node_23 578 T39971_node_31 579 T39971_node_33 580 T39971_node_7 581 T39971_node_1 582 T39971_node_10 583 T39971_node_11 584 T39971_node_12 585 T39971_node_15 586 T39971_node_16 587 T39971_node_17 588 T39971_node_26 589 T39971_node_27 590 T39971_node_28 591 T39971_node_29 592 T39971_node_3 593 T39971_node_30 594 T39971_node_34 595 T39971_node_35 596 T39971_node_36 597 T39971_node_4 598 T39971_node_5 599 T39971_node_8 600 T39971_node_9 601

TABLE 3 Proteins of interest Protein Name SEQ ID NO: T39971_P6 603 T39971_P9 604 T39971_P11 605 T39971_P12 606

These sequences are variants of the known protein Vitronectin precursor (SwissProt accession identifier VTNC_HUMAN; known also according to the synonyms Serum spreading factor; S-protein; V75), SEQ ID NO: 602, referred to herein as the previously known protein.

Protein Vitronectin precursor is known or believed to have the following function(s): Vitronectin is a cell adhesion and spreading factor found in serum and tissues. Vitronectin interacts with glycosaminoglycans and proteoglycans. Is recognized by certain members of the integrin family and serves as a cell-to-substrate adhesion molecule. Inhibitor of the membrane-damaging effect of the terminal cytolytic complement pathway. The sequence for protein Vitronectin precursor is given at the end of the application, as “Vitronectin precursor amino acid sequence”. Known polymorphisms for this sequence are as shown in Table 4.

TABLE 4 Amino acid mutations for Known Protein SNP position(s) on amino acid sequence Comment 122 A -> S. /FTId = VAR_012983. 268 R -> Q. /FTId = VAR_012984. 400 T -> M. /FTId = VAR_012985. 50 C -> N 225 S -> N 366 A -> T

Protein Vitronectin precursor localization is believed to be Extracellular.

The previously known protein also has the following indication(s) and/or potential therapeutic use(s): Cancer, melanoma. It has been investigated for clinical/therapeutic use in humans, for example as a target for an antibody or small molecule, and/or as a direct therapeutic; available information related to these investigations is as follows. Potential pharmaceutically related or therapeutically related activity or activities of the previously known protein are as follows: Alphavbeta3 integrin antagonist; Apoptosis agonist. A therapeutic role for a protein represented by the cluster has been predicted. The cluster was assigned this field because there was information in the drug database or the public databases (e.g., described herein above) that this protein, or part thereof, is used or can be used for a potential therapeutic indication: Anticancer.

The following GO Annotation(s) apply to the previously known protein. The following annotation(s) were found: immune response; cell adhesion, which are annotation(s) related to Biological Process; protein binding; heparin binding, which are annotation(s) related to Molecular Function; and extracellular space, which are annotation(s) related to Cellular Component.

The GO assignment relies on information from one or more of the SwissProt/TremBl Protein knowledgebase, available from <http://www.expasy.ch/sprot/>; or Locuslink, available from <http://www.ncbi.nlm.nih.gov/projects/LocusLink/>.

Cluster T39971 can be used as a diagnostic marker according to overexpression of transcripts of this cluster in cancer. Expression of such transcripts in normal tissues is also given according to the previously described methods. The term “number” in the right hand column of the table and the numbers on the y-axis of FIG. 29 refer to weighted expression of ESTs in each category, as “parts per million” (ratio of the expression of ESTs for a particular cluster to the expression of all ESTs in that category, according to parts per million).

Overall, the following results were obtained as shown with regard to the histograms in FIG. 29 and Table 5. This cluster is overexpressed (at least at a minimum level) in the following pathological conditions: liver cancer, lung malignant tumors and pancreas carcinoma.

TABLE 5 Normal tissue distribution Name of Tissue Number adrenal 60 bladder 0 bone 0 brain 9 colon 0 epithelial 79 general 29 liver 2164 lung 0 lymph nodes 0 breast 0 pancreas 0 prostate 0 skin 0 uterus 0

TABLE 6 P values and ratios for expression in cancerous tissue Name of Tissue P1 P2 SP1 R3 SP2 R4 adrenal 6.9e−01 7.4e−01 2.0e−02 2.3 5.3e−02 1.8 bladder 5.4e−01 6.0e−01 5.6e−01 1.8 6.8e−01 1.5 bone 1 6.7e−01 1 1.0 7.0e−01 1.4 brain 8.0e−01 8.6e−01 3.0e−01 1.9 5.3e−01 1.2 colon 4.2e−01 4.8e−01 7.0e−01 1.6 7.7e−01 1.4 epithelial 6.6e−01 5.7e−01 1.0e−01 0.8 8.7e−01 0.6 general 5.1e−01 3.8e−01 9.2e−08 1.6 8.3e−04 1.3 liver 1 6.7e−01 2.3e−03 0.3 1 0.2 lung 2.4e−01 9.1e−02 1.7e−01 4.3 8.1e−03 5.0 lymph nodes 1 5.7e−01 1 1.0 5.8e−01 2.3 breast 1 6.7e−01 1 1.0 8.2e−01 1.2 pancreas 9.5e−02 1.8e−01 1.5e−11 6.5 8.2e−09 4.6 prostate 7.3e−01 6.0e−01 6.7e−01 1.5 5.6e−01 1.7 skin 1 4.4e−01 1 1.0 6.4e−01 1.6 uterus 5.0e−01 2.6e−01 1 1.1 8.0e−01 1.4

As noted above, cluster T39971 features 4 transcript(s), which were listed in Table 1 above. These transcript(s) encode for protein(s) which are variant(s) of protein Vitronectin precursor. A description of each variant protein according to the present invention is now provided.

Variant protein T39971_P6 (SEQ ID NO: 603) according to the present invention has an amino acid sequence as given at the end of the application; it is encoded by transcript(s) T39971_T5 (SEQ ID NO: 573). An alignment is given to the known protein (Vitronectin precursor) at the end of the application. One or more alignments to one or more previously published protein sequences are given at the end of the application. A brief description of the relationship of the variant protein according to the present invention to each such aligned protein is as follows:

Comparison report between T39971_P6 (SEQ ID NO: 603) and VTNC_HUMAN:

1. An isolated chimeric polypeptide encoding for T39971_P6 (SEQ ID NO: 603), comprising a first amino acid sequence being at least 90% homologous to MAPLRPLLILALLAWVALADQESCKGRCTEGFNVDKKCQCDELCSYYQSCCTDYTAEC KPQVTRGDVFTMPEDEYTVYDDGEEKNNATVHEQVGGPSLTSDLQAQSKGNPEQTPV LKPEEEAPAPEVGASKPEGIDSRPETLHPGRPQPPAEEELCSGKPFDAFTDLKNGSLFAFR GQYCYELDEKAVRPGYPKLIRDVWGIEGPIDAAFTRINCQGKTYLFKGSQYWRFEDGV LDPDYPRNISDGFDGIPDNVDAALALPAHSYSGRERVYFFKG corresponding to amino acids 1-276 of VTNC_HUMAN, which also corresponds to amino acids 1-276 of T39971_P6 (SEQ ID NO: 603), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence TQGVVGD (SEQ ID NO: 1075) corresponding to amino acids 277-283 of T39971_P6 (SEQ ID NO: 603), wherein said first and second amino acid sequences are contiguous and in a sequential order.

2. An isolated polypeptide encoding for a tail of T39971_P6 (SEQ ID NO: 603), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence TQGVVGD (SEQ ID NO: 1075) in T39971_P6 (SEQ ID NO: 603). The location of the variant protein was determined according to results from a number of different software programs and analyses, including analyses from SignalP and other specialized programs. The variant protein is believed to be located as follows with regard to the cell: secreted. The protein localization is believed to be secreted because both signal-peptide prediction programs predict that this protein has a signal peptide, and neither trans-membrane region prediction program predicts that this protein has a trans-membrane region.

Variant protein T39971_P6 (SEQ ID NO: 603) also has the following non-silent SNPs (Single Nucleotide Polymorphisms) as listed in Table 7, (given according to their position(s) on the amino acid sequence, with the alternative amino acid(s) listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein T39971_P6 (SEQ ID NO: 603) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 7 Amino acid mutations SNP position(s) on amino acid sequence Alternative amino acid(s) Previously known SNP? 122 A -> S Yes 145 G -> No 268 R -> Q Yes 280 V -> A Yes 180 C -> No 180 C -> W No 192 Y -> No 209 A -> No 211 T -> No 267 G -> No 267 G -> A No 268 R -> No

Variant protein T39971_P6 (SEQ ID NO: 603) is encoded by the following transcript(s): T39971_T5 (SEQ ID NO: 573), for which the sequence(s) is/are given at the end of the application. The coding portion of transcript T39971_T5 (SEQ ID NO: 573) is shown in bold; this coding portion starts at position 756 and ends at position 1604. The transcript also has the following SNPs as listed in Table 8 (given according to their position on the nucleotide sequence, with the alternative nucleic acid listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein T39971_P6 (SEQ ID NO: 603) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 8 Nucleic acid SNPs SNP position on nucleotide sequence Alternative nucleic acid Previously known SNP? 417 G -> C Yes 459 T -> C Yes 1387 C -> No 1406 -> A No 1406 -> G No 1555 G -> No 1555 G -> C No 1558 G -> No 1558 G -> A Yes 1594 T -> C Yes 1642 T -> C Yes 1770 C -> T Yes 529 G -> T Yes 1982 A -> G No 2007 G -> No 2029 T -> C No 2094 T -> C No 2117 C -> G No 2123 C -> T Yes 2152 C -> T Yes 2182 G -> T No 2185 A -> C No 2297 T -> C Yes 1119 G -> T Yes 2411 G -> No 2411 G -> T No 2487 T -> C Yes 1188 G -> No 1295 C -> No 1295 C -> G No 1324 -> T No 1331 C -> No 1381 C -> No

Variant protein T39971_P9 (SEQ ID NO: 604) according to the present invention has an amino acid sequence as given at the end of the application; it is encoded by transcript(s) T39971_T10 (SEQ ID NO: 570). An alignment is given to the known protein (Vitronectin precursor) at the end of the application. One or more alignments to one or more previously published protein sequences are given at the end of the application. A brief description of the relationship of the variant protein according to the present invention to each such aligned protein is as follows:

Comparison report between T39971_P9 (SEQ ID NO: 604) and VTNC_HUMAN:

1. An isolated chimeric polypeptide encoding for T39971_P9 (SEQ ID NO: 604), comprising a first amino acid sequence being at least 90% homologous to MAPLRPLLILALLAWVALADQESCKGRCTEGFNVDKKCQCDELCSYYQSCCTDYTAEC KPQVTRGDVFTMPEDEYTVYDDGEEKNNATVHEQVGGPSLTSDLQAQSKGNPEQTPV LKPEEEAPAPEVGASKPEGIDSRPETLHPGRPQPPAEEELCSGKPFDAFTDLKNGSLFAFR GQYCYELDEKAVRPGYPKLIRDVWGIEGPIDAAFTRINCQGKTYLFKGSQYWRFEDGV LDPDYPRNISDGFDGIPDNVDAALALPAHSYSGRERVYFFKGKQYWEYQFQHQPSQEE CEGSSLSAVFEHFAMMQRDSWEDIFELLFWGRT corresponding to amino acids 1-325 of VTNC_HUMAN, which also corresponds to amino acids 1-325 of T39971_P9 (SEQ ID NO: 604), and a second amino acid sequence being at least 90% homologous to SGMAPRPSLAKKQRFRHRNRKGYRSQRGHSRGRNQNSRRPSRATWLSLFSSEESNLGA NNYDDYRMDWLVPATCEPIQSVFFFSGDKYYRVNLRTRRVDTVDPPYPRSIAQYWLGC PAPGHL corresponding to amino acids 357-478 of VTNC_HUMAN, which also corresponds to amino acids 326-447 of T39971_P9 (SEQ ID NO: 604), wherein said first and second amino acid sequences are contiguous and in a sequential order.

2. An isolated chimeric polypeptide encoding for an edge portion of T39971_P9 (SEQ ID NO: 604), comprising a polypeptide having a length “n”, wherein n is at least about 10 amino acids in length, optionally at least about 20 amino acids in length, preferably at least about 30 amino acids in length, more preferably at least about 40 amino acids in length and most preferably at least about 50 amino acids in length, wherein at least two amino acids comprise TS, having a structure as follows: a sequence starting from any of amino acid numbers 325-x to 325; and ending at any of amino acid numbers 326+((n−2)−x), in which x varies from 0 to n−2.

The location of the variant protein was determined according to results from a number of different software programs and analyses, including analyses from SignalP and other specialized programs. The variant protein is believed to be located as follows with regard to the cell: secreted. The protein localization is believed to be secreted because both signal-peptide prediction programs predict that this protein has a signal peptide, and neither trans-membrane region prediction program predicts that this protein has a trans-membrane region.

Variant protein T39971_P9 (SEQ ID NO: 604) also has the following non-silent SNPs (Single Nucleotide Polymorphisms) as listed in Table 9, (given according to their position(s) on the amino acid sequence, with the alternative amino acid(s) listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein T39971_P9 (SEQ ID NO: 604) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 9 Amino acid mutations SNP position(s) on amino acid sequence Alternative amino acid(s) Previously known SNP? 122 A -> S Yes 145 G -> No 268 R -> Q Yes 328 M -> T No 350 S -> P No 369 T -> M Yes 379 S -> I No 380 N -> T No 180 C -> No 180 C -> W No 192 Y -> No 209 A -> No 211 T -> No 267 G -> No 267 G -> A No 268 R -> No

Variant protein T39971_P9 (SEQ ID NO: 604) is encoded by the following transcript(s): T39971_T10 (SEQ ID NO: 570), for which the sequence(s) is/are given at the end of the application. The coding portion of transcript T39971_T10 (SEQ ID NO: 570) is shown in bold; this coding portion starts at position 756 and ends at position 2096. The transcript also has the following SNPs as listed in Table 10 (given according to their position on the nucleotide sequence, with the alternative nucleic acid listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein T39971_P9 (SEQ ID NO: 604) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 10 Nucleic acid SNPs SNP position on nucleotide sequence Alternative nucleic acid Previously known SNP? 417 G -> C Yes 459 T -> C Yes 1387 C -> No 1406 -> A No 1406 -> G No 1555 G -> No 1555 G -> C No 1558 G -> No 1558 G -> A Yes 1738 T -> C No 1803 T -> C No 1826 C -> G No 529 G -> T Yes 1832 C -> T Yes 1861 C -> T Yes 1891 G -> T No 1894 A -> C No 2006 T -> C Yes 2120 G -> No 2120 G -> T No 2196 T -> C Yes 1119 G -> T Yes 1188 G -> No 1295 C -> No 1295 C -> G No 1324 -> T No 1331 C -> No 1381 C -> No

Variant protein T39971_P11 (SEQ ID NO: 605) according to the present invention has an amino acid sequence as given at the end of the application; it is encoded by transcript(s) T39971_T12 (SEQ ID NO: 571). An alignment is given to the known protein (Vitronectin precursor) at the end of the application. One or more alignments to one or more previously published protein sequences are given at the end of the application. A brief description of the relationship of the variant protein according to the present invention to each such aligned protein is as follows:

Comparison report between T39971_P11 (SEQ ID NO: 605) and VTNC_HUMAN:

1. An isolated chimeric polypeptide encoding for T39971_P11 (SEQ ID NO: 605), comprising a first amino acid sequence being at least 90% homologous to MAPLRPLLILALLAWVALADQESCKGRCTEGFNVDKKCQCDELCSYYQSCCTDYTAEC KPQVTRGDVFTMPEDEYTVYDDGEEKNNATVHEQVGGPSLTSDLQAQSKGNPEQTPV LKPEEEAPAPEVGASKPEGIDSRPETLHPGRPQPPAEEELCSGKPFDAFTDLKNGSLFAFR GQYCYELDEKAVRPGYPKLIRDVWGIEGPIDAAFTRINCQGKTYLFKGSQYWRFEDGV LDPDYPRNISDGFDGIPDNVDAALALPAHSYSGRERVYFFKGKQYWEYQFQHQPSQEE CEGSSLSAVFEHFAMMQRDSWEDIFELLFWGRTS corresponding to amino acids 1-326 of VTNC_HUMAN, which also corresponds to amino acids 1-326 of T39971_P11 (SEQ ID NO: 605), and a second amino acid sequence being at least 90% homologous to DKYYRVNLRTRRVDTVDPPYPRSIAQYWLGCPAPGHL corresponding to amino acids 442-478 of VTNC_HUMAN, which also corresponds to amino acids 327-363 of T39971_P11 (SEQ ID NO: 605), wherein said first and second amino acid sequences are contiguous and in a sequential order.

2. An isolated chimeric polypeptide encoding for an edge portion of T39971_P11 (SEQ ID NO: 605), comprising a polypeptide having a length “n”, wherein n is at least about 10 amino acids in length, optionally at least about 20 amino acids in length, preferably at least about 30 amino acids in length, more preferably at least about 40 amino acids in length and most preferably at least about 50 amino acids in length, wherein at least two amino acids comprise SD, having a structure as follows: a sequence starting from any of amino acid numbers 326-x to 326; and ending at any of amino acid numbers 327+((n−2)−x), in which x varies from 0 to n−2.

Comparison report between T39971_P11 (SEQ ID NO: 605) and Q9BSH7 (SEQ ID NO: 1000) (SEQ ID NO:1000):

1. An isolated chimeric polypeptide encoding for T39971_P11 (SEQ ID NO: 605), comprising a first amino acid sequence being at least 90% homologous to MAPLRPLLILALLAWVALADQESCKGRCTEGFNVDKKCQCDELCSYYQSCCTDYTAEC KPQVTRGDVFTMPEDEYTVYDDGEEKNNATVHEQVGGPSLTSDLQAQSKGNPEQTPV LKPEEEAPAPEVGASKPEGIDSRPETLHPGRPQPPAEEELCSGKPFDAFTDLKNGSLFAFR GQYCYELDEKAVRPGYPKLIRDVWGIEGPIDAAFTRINCQGKTYLFKGSQYWRFEDGV LDPDYPRNISDGFDGIPDNVDAALALPAHSYSGRERVYFFKGKQYWEYQFQHQPSQEE CEGSSLSAVFEHFAMMQRDSWEDIFELLFWGRTS corresponding to amino acids 1-326 of Q9BSH7 (SEQ ID NO: 1000), which also corresponds to amino acids 1-326 of T39971_P11 (SEQ ID NO: 605), and a second amino acid sequence being at least 90% homologous to DKYYRVNLRTRRVDTVDPPYPRSIAQYWLGCPAPGHL corresponding to amino acids 442-478 of Q9BSH7 (SEQ ID NO: 1000), which also corresponds to amino acids 327-363 of T39971_P11 (SEQ ID NO: 605), wherein said first and second amino acid sequences are contiguous and in a sequential order.

2. An isolated chimeric polypeptide encoding for an edge portion of T39971_P11 (SEQ ID NO: 605), comprising a polypeptide having a length “n”, wherein n is at least about 10 amino acids in length, optionally at least about 20 amino acids in length, preferably at least about 30 amino acids in length, more preferably at least about 40 amino acids in length and most preferably at least about 50 amino acids in length, wherein at least two amino acids comprise SD, having a structure as follows: a sequence starting from any of amino acid numbers 326-x to 326; and ending at any of amino acid numbers 327+((n−2)−x), in which x varies from 0 to n−2.

The location of the variant protein was determined according to results from a number of different software programs and analyses, including analyses from SignalP and other specialized programs. The variant protein is believed to be located as follows with regard to the cell: secreted. The protein localization is believed to be secreted because both signal-peptide prediction programs predict that this protein has a signal peptide, and neither trans-membrane region prediction program predicts that this protein has a trans-membrane region.

Variant protein T39971_P11 (SEQ ID NO: 605) also has the following non-silent SNPs (Single Nucleotide Polymorphisms) as listed in Table 11, (given according to their position(s) on the amino acid sequence, with the alternative amino acid(s) listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein T39971_P11 (SEQ ID NO: 605) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 11 Amino acid mutations SNP position(s) on amino acid sequence Alternative amino acid(s) Previously known SNP? 122 A -> S Yes 145 G -> No 268 R -> Q Yes 180 C -> No 180 C -> W No 192 Y -> No 209 A -> No 211 T -> No 267 G -> No 267 G -> A No 268 R -> No

Variant protein T39971_P11 (SEQ ID NO: 605) is encoded by the following transcript(s): T39971_T12 (SEQ ID NO: 571), for which the sequence(s) is/are given at the end of the application. The coding portion of transcript T39971_T12 (SEQ ID NO: 571) is shown in bold; this coding portion starts at position 756 and ends at position 1844. The transcript also has the following SNPs as listed in Table 12 (given according to their position on the nucleotide sequence, with the alternative nucleic acid listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein T39971_P11 (SEQ ID NO: 605) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 12 Nucleic acid SNPs SNP position on nucleotide sequence Alternative nucleic acid Previously known SNP? 417 G -> C Yes 459 T -> C Yes 1387 C -> No 1406 -> A No 1406 -> G No 1555 G -> No 1555 G -> C No 1558 G -> No 1558 G -> A Yes 1754 T -> C Yes 1868 G -> No 1868 G -> T No 529 G -> T Yes 1944 T -> C Yes 1119 G -> T Yes 1188 G -> No 1295 C -> No 1295 C -> G No 1324 -> T No 1331 C -> No 1381 C -> No

Variant protein T39971_P12 (SEQ ID NO: 606) according to the present invention has an amino acid sequence as given at the end of the application; it is encoded by transcript(s) T39971_T16 (SEQ ID NO: 572). An alignment is given to the known protein (Vitronectin precursor) at the end of the application. One or more alignments to one or more previously published protein sequences are given at the end of the application. A brief description of the relationship of the variant protein according to the present invention to each such aligned protein is as follows:

Comparison report between T39971_P12 (SEQ ID NO: 606) and VTNC_HUMAN:

1. An isolated chimeric polypeptide encoding for T39971_P12 (SEQ ID NO: 606), comprising a first amino acid sequence being at least 90% homologous to MAPLRPLLILALLAWVALADQESCKGRCTEGFNVDKKCQCDELCSYYQSCCTDYTAEC KPQVTRGDVFTMPEDEYTVYDDGEEKNNATVHEQVGGPSLTSDLQAQSKGNPEQTPV LKPEEEAPAPEVGASKPEGIDSRPETLHPGRPQPPAEEELCSGKPFDAFTDLKNGSLFAFR GQYCYELDEKAVRPGYPKLIRDVWGIEGPIDAAFTRINCQGKTYLFK corresponding to amino acids 1-223 of VTNC_HUMAN, which also corresponds to amino acids 1-223 of T39971_P12 (SEQ ID NO: 606), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence VPGAVGQGRKHLGRV (SEQ ID NO: 1076) corresponding to amino acids 224-238 of T39971_P12 (SEQ ID NO: 606), wherein said first and second amino acid sequences are contiguous and in a sequential order.

2. An isolated polypeptide encoding for a tail of T39971_P12 (SEQ ID NO: 606), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence VPGAVGQGRKHLGRV (SEQ ID NO: 1076) in T39971_P12 (SEQ ID NO: 606).

Comparison report between T39971_P12 (SEQ ID NO: 606) and Q9BSH7 (SEQ ID NO: 1000):

1. An isolated chimeric polypeptide encoding for T39971_P12 (SEQ ID NO: 606), comprising a first amino acid sequence being at least 90% homologous to MAPLRPLLILALLAWVALADQESCKGRCTEGFNVDKKCQCDELCSYYQSCCTDYTAEC KPQVTRGDVFTMPEDEYTVYDDGEEKNNATVHEQVGGPSLTSDLQAQSKGNPEQTPV LKPEEEAPAPEVGASKPEGIDSRPETLHPGRPQPPAEEELCSGKPFDAFTDLKNGSLFAFR GQYCYELDEKAVRPGYPKLIRDVWGIEGPIDAAFTRINCQGKTYLFK corresponding to amino acids 1-223 of Q9BSH7 (SEQ ID NO: 1000), which also corresponds to amino acids 1-223 of T39971_P12 (SEQ ID NO: 606), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence VPGAVGQGRKHLGRV (SEQ ID NO: 1076) corresponding to amino acids 224-238 of T39971_P12 (SEQ ID NO: 606), wherein said first and second amino acid sequences are contiguous and in a sequential order.

2. An isolated polypeptide encoding for a tail of T39971_P12 (SEQ ID NO: 606), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence VPGAVGQGRKHLGRV (SEQ ID NO: 1076) in T39971_P12 (SEQ ID NO: 606).

The location of the variant protein was determined according to results from a number of different software programs and analyses, including analyses from SignalP and other specialized programs. The variant protein is believed to be located as follows with regard to the cell: secreted. The protein localization is believed to be secreted because both signal-peptide prediction programs predict that this protein has a signal peptide, and neither trans-membrane region prediction program predicts that this protein has a trans-membrane region.

Variant protein T39971_P12 (SEQ ID NO: 606) also has the following non-silent SNPs (Single Nucleotide Polymorphisms) as listed in Table 13, (given according to their position(s) on the amino acid sequence, with the alternative amino acid(s) listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein T39971_P12 (SEQ ID NO: 606) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 13 Amino acid mutations SNP position(s) on amino acid sequence Alternative amino acid(s) Previously known SNP? 122 A -> S Yes 145 G -> No 180 C -> No 180 C -> W No 192 Y -> No 209 A -> No 211 T -> No

Variant protein T39971_P12 (SEQ ID NO: 606) is encoded by the following transcript(s): T39971_T16 (SEQ ID NO: 572), for which the sequence(s) is/are given at the end of the application. The coding portion of transcript T39971_T16 (SEQ ID NO: 572) is shown in bold; this coding portion starts at position 756 and ends at position 1469. The transcript also has the following SNPs as listed in Table 14 (given according to their position on the nucleotide sequence, with the alternative nucleic acid listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein T39971_P12 (SEQ ID NO: 606) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 14 Nucleic acid SNPs SNP position on nucleotide sequence Alternative nucleic acid Previously known SNP? 417 G -> C Yes 459 T -> C Yes 1387 C -> No 1406 -> A No 1406 -> G No 529 G -> T Yes 1119 G -> T Yes 1188 G -> No 1295 C -> No 1295 C -> G No 1324 -> T No 1331 C -> No 1381 C -> No

As noted above, cluster T39971 features 28 segment(s), which were listed in Table 2 above and for which the sequence(s) are given at the end of the application. These segment(s) are portions of nucleic acid sequence(s) which are described herein separately because they are of particular interest. A description of each segment according to the present invention is now provided.

Segment cluster T39971_node_(—)0 (SEQ ID NO: 574) according to the present invention is supported by 76 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): T39971_T10 (SEQ ID NO: 570), T39971_T12 (SEQ ID NO: 571), T39971_T16 (SEQ ID NO: 572) and T39971_T5 (SEQ ID NO: 573). Table 15 below describes the starting and ending position of this segment on each transcript.

TABLE 15 Segment location on transcripts Segment Segment Transcript starting ending name position position T39971_T10 1 810 (SEQ ID NO:570) T39971_T12 1 810 (SEQ ID NO:571) T39971_T16 1 810 (SEQ ID NO:572) T39971_T5 1 810 (SEQ ID NO:573)

Segment cluster T39971_node_(—)18 (SEQ ID NO: 575) according to the present invention is supported by 1 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): T39971_T16 (SEQ ID NO: 572). Table 16 below describes the starting and ending position of this segment on each transcript.

TABLE 16 Segment location on transcripts Segment Segment Transcript starting ending name position position T39971_T16 1425 1592 (SEQ ID NO:572)

Segment cluster T39971_node_(—)21 (SEQ ID NO: 576) according to the present invention is supported by 99 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): T39971_T10 (SEQ ID NO: 570), T39971_T12 (SEQ ID NO: 571) and T39971_T5 (SEQ ID NO: 573). Table 17 below describes the starting and ending position of this segment on each transcript.

TABLE 17 Segment location on transcripts Segment Segment Transcript starting ending name position position T39971_T10 1425 1581 (SEQ ID NO:570) T39971_T12 1425 1581 (SEQ ID NO:571) T39971_T5 1425 1581 (SEQ ID NO:573)

Segment cluster T39971_node_(—)22 (SEQ ID NO: 577) according to the present invention is supported by 7 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): T39971_T5 (SEQ ID NO: 573). Table 18 below describes the starting and ending position of this segment on each transcript.

TABLE 18 Segment location on transcripts Segment Segment Transcript starting ending name position position T39971_T5 1582 1779 (SEQ ID NO:573)

Segment cluster T39971_node_(—)23 (SEQ ID NO: 578) according to the present invention is supported by 101 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): T39971_T10 (SEQ ID NO: 570), T39971_T12 (SEQ ID NO: 571) and T39971_T5 (SEQ ID NO: 573). Table 19 below describes the starting and ending position of this segment on each transcript.

TABLE 19 Segment location on transcripts Segment Segment Transcript starting ending name position position T39971_T10 1582 1734 (SEQ ID NO:570) T39971_T12 1582 1734 (SEQ ID NO:571) T39971_T5 1780 1932 (SEQ ID NO:573)

Segment cluster T39971_node_(—)31 (SEQ ID NO: 579) according to the present invention is supported by 94 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): T39971_T10 (SEQ ID NO: 570) and T39971_T5 (SEQ ID NO: 573). Table 20 below describes the starting and ending position of this segment on each transcript.

TABLE 20 Segment location on transcripts Segment Segment Transcript starting ending name position position T39971_T10 1847 1986 (SEQ ID NO:570) T39971_T5 2138 2277 (SEQ ID NO:573)

Segment cluster T39971_node_(—)33 (SEQ ID NO: 580) according to the present invention is supported by 77 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): T39971_T10 (SEQ ID NO: 570), T39971_T12 (SEQ ID NO: 571) and T39971_T5 (SEQ ID NO: 573). Table 21 below describes the starting and ending position of this segment on each transcript.

TABLE 21 Segment location on transcripts Segment Segment Transcript starting ending name position position T39971_T10 1987 2113 (SEQ ID NO:570) T39971_T12 1735 1861 (SEQ ID NO:571) T39971_T5 2278 2404 (SEQ ID NO:573)

Segment cluster T39971_node_(—)7 (SEQ ID NO: 581) according to the present invention is supported by 87 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): T39971_T10 (SEQ ID NO: 570), T39971_T12 (SEQ ID NO: 571), T39971_T16 (SEQ ID NO: 572) and T39971_T5 (SEQ ID NO: 573). Table 22 below describes the starting and ending position of this segment on each transcript.

TABLE 22 Segment location on transcripts Segment Segment Transcript starting ending name position position T39971_T10 940 1162 (SEQ ID NO:570) T39971_T12 940 1162 (SEQ ID NO:571) T39971_T16 940 1162 (SEQ ID NO:572) T39971_T5 940 1162 (SEQ ID NO:573)

According to an optional embodiment of the present invention, short segments related to the above cluster are also provided. These segments are up to about 120 bp in length, and so are included in a separate description.

Segment cluster T39971_node_(—)1 (SEQ ID NO: 582) according to the present invention can be found in the following transcript(s): T39971_T10 (SEQ ID NO: 570), T39971_T12 (SEQ ID NO: 571), T39971_T16 (SEQ ID NO: 572) and T39971_T5 (SEQ ID NO: 573). Table 23 below describes the starting and ending position of this segment on each transcript.

TABLE 23 Segment location on transcripts Segment Segment Transcript starting ending name position position T39971_T10 811 819 (SEQ ID NO:570) T39971_T12 811 819 (SEQ ID NO:571) T39971_T16 811 819 (SEQ ID NO:572) T39971_T5 811 819 (SEQ ID NO:573)

Segment cluster T39971_node_(—)10 (SEQ ID NO: 583) according to the present invention is supported by 77 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): T39971_T10 (SEQ ID NO: 570), T39971_T12 (SEQ ID NO: 571), T39971_T16 (SEQ ID NO: 572) and T39971_T5 (SEQ ID NO: 573). Table 24 below describes the starting and ending position of this segment on each transcript.

TABLE 24 Segment location on transcripts Segment Segment Transcript starting ending name position position T39971_T10 1189 1232 (SEQ ID NO:570) T39971_T12 1189 1232 (SEQ ID NO:571) T39971_T16 1189 1232 (SEQ ID NO:572) T39971_T5 1189 1232 (SEQ ID NO:573)

Segment cluster T39971_node_(—)11 (SEQ ID NO: 584) according to the present invention is supported by 79 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): T39971_T10 (SEQ ID NO: 570), T39971_T12 (SEQ ID NO: 571), T39971_T16 (SEQ ID NO: 572) and T39971_T5 (SEQ ID NO: 573). Table 25 below describes the starting and ending position of this segment on each transcript.

TABLE 25 Segment location on transcripts Segment Segment Transcript starting ending name position position T39971_T10 1233 1270 (SEQ ID NO:570) T39971_T12 1233 1270 (SEQ ID NO:571) T39971_T16 1233 1270 (SEQ ID NO:572) T39971_T5 1233 1270 (SEQ ID NO:573)

Segment cluster T39971_node_(—)12 (SEQ ID NO: 585) according to the present invention can be found in the following transcript(s): T39971_T10 (SEQ ID NO: 570), T39971_T12 (SEQ ID NO: 571), T39971_T16 (SEQ ID NO: 572) and T39971_T5 (SEQ ID NO: 573). Table 26 below describes the starting and ending position of this segment on each transcript.

TABLE 26 Segment location on transcripts Segment Segment Transcript starting ending name position position T39971_T10 1271 1284 (SEQ ID NO:570) T39971_T12 1271 1284 (SEQ ID NO:571) T39971_T16 1271 1284 (SEQ ID NO:572) T39971_T5 1271 1284 (SEQ ID NO:573)

Segment cluster T39971_node_(—)15 (SEQ ID NO: 586) according to the present invention is supported by 79 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): T39971_T10 (SEQ ID NO: 570), T39971_T12 (SEQ ID NO: 571), T39971_T16 (SEQ ID NO: 572) and T39971_T5 (SEQ ID NO: 573). Table 27 below describes the starting and ending position of this segment on each transcript.

TABLE 27 Segment location on transcripts Segment Segment Transcript starting ending name position position T39971_T10 1285 1316 (SEQ ID NO:570) T39971_T12 1285 1316 (SEQ ID NO:571) T39971_T16 1285 1316 (SEQ ID NO:572) T39971_T5 1285 1316 (SEQ ID NO:573)

Segment cluster T39971_node_(—)16 (SEQ ID NO: 587) according to the present invention can be found in the following transcript(s): T39971_T10 (SEQ ID NO: 570), T39971_T12 (SEQ ID NO: 571), T39971_T16 (SEQ ID NO: 572) and T39971_T5 (SEQ ID NO: 573). Table 28 below describes the starting and ending position of this segment on each transcript.

TABLE 28 Segment location on transcripts Segment Segment Transcript starting ending name position position T39971_T10 1317 1340 (SEQ ID NO:570) T39971_T12 1317 1340 (SEQ ID NO:571) T39971_T16 1317 1340 (SEQ ID NO:572) T39971_T5 1317 1340 (SEQ ID NO:573)

Segment cluster T39971_node_(—)17 (SEQ ID NO: 588) according to the present invention is supported by 86 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): T39971_T10 (SEQ ID NO: 570), T39971_T12 (SEQ ID NO: 571), T39971_T16 (SEQ ID NO: 572) and T39971_T5 (SEQ ID NO: 573). Table 29 below describes the starting and ending position of this segment on each transcript.

TABLE 29 Segment location on transcripts Segment Segment Transcript starting ending name position position T39971_T10 1341 1424 (SEQ ID NO:570) T39971_T12 1341 1424 (SEQ ID NO:571) T39971_T16 1341 1424 (SEQ ID NO:572) T39971_T5 1341 1424 (SEQ ID NO:573)

Segment cluster T39971_node_(—)26 (SEQ ID NO: 589) according to the present invention is supported by 85 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): T39971_T5 (SEQ ID NO: 573). Table 30 below describes the starting and ending position of this segment on each transcript.

TABLE 30 Segment location on transcripts Segment Segment Transcript starting ending name position position T39971_T5 1933 1974 (SEQ ID NO:573)

Segment cluster T39971_node_(—)27 (SEQ ID NO: 590) according to the present invention is supported by 90 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): T39971_T5 (SEQ ID NO: 573). Table 31 below describes the starting and ending position of this segment on each transcript.

TABLE 31 Segment location on transcripts Segment Segment Transcript starting ending name position position T39971_T5 1975 2025 (SEQ ID NO:573)

Segment cluster T39971_node_(—)28 (SEQ ID NO: 591) according to the present invention can be found in the following transcript(s): T39971_T10 (SEQ ID NO: 570) and T39971_T5 (SEQ ID NO: 573). Table 32 below describes the starting and ending position of this segment on each transcript.

TABLE 32 Segment location on transcripts Segment Segment Transcript starting ending name position position T39971_T10 1735 1743 (SEQ ID NO:570) T39971_T5 2026 2034 (SEQ ID NO:573)

Segment cluster T39971_node_(—)29 (SEQ ID NO: 592) according to the present invention is supported by 99 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): T39971_T10 (SEQ ID NO: 570) and T39971_T5 (SEQ ID NO: 573). Table 33 below describes the starting and ending position of this segment on each transcript.

TABLE 33 Segment location on transcripts Segment Segment Transcript starting ending name position position T39971_T10 1744 1838 (SEQ ID NO:570) T39971_T5 2035 2129 (SEQ ID NO:573)

Segment cluster T39971_node_(—)3 (SEQ ID NO: 593) according to the present invention is supported by 78 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): T39971_T10 (SEQ ID NO: 570), T39971_T12 (SEQ ID NO: 571), T39971_T16 (SEQ ID NO: 572) and T39971_T5 (SEQ ID NO: 573). Table 34 below describes the starting and ending position of this segment on each transcript.

TABLE 34 Segment location on transcripts Segment Segment Transcript starting ending name position position T39971_T10 820 861 (SEQ ID NO:570) T39971_T12 820 861 (SEQ ID NO:571) T39971_T16 820 861 (SEQ ID NO:572) T39971_T5 820 861 (SEQ ID NO:573)

Segment cluster T39971_node_(—)30 (SEQ ID NO: 594) according to the present invention can be found in the following transcript(s): T39971_T10 (SEQ ID NO: 570) and T39971_T5 (SEQ ID NO: 573). Table 35 below describes the starting and ending position of this segment on each transcript.

TABLE 35 Segment location on transcripts Segment Segment Transcript starting ending name position position T39971_T10 1839 1846 (SEQ ID NO:570) T39971_T5 2130 2137 (SEQ ID NO:573)

Segment cluster T39971_node_(—)34 (SEQ ID NO: 595) according to the present invention can be found in the following transcript(s): T39971_T10 (SEQ ID NO: 570), T39971_T12 (SEQ ID NO: 571) and T39971_T5 (SEQ ID NO: 573). Table 36 below describes the starting and ending position of this segment on each transcript.

TABLE 36 Segment location on transcripts Segment Segment Transcript starting ending name position position T39971_T10 2114 2120 (SEQ ID NO:570) T39971_T12 1862 1868 (SEQ ID NO:571) T39971_T5 2405 2411 (SEQ ID NO:573)

Segment cluster T39971_node_(—)35 (SEQ ID NO: 596) according to the present invention can be found in the following transcript(s): T39971_T10 (SEQ ID NO: 570), T39971_T12 (SEQ ID NO: 571) and T39971_T5 (SEQ ID NO: 573). Table 37 below describes the starting and ending position of this segment on each transcript.

TABLE 37 Segment location on transcripts Segment Segment Transcript name starting position ending position T39971_T10 2121 2137 (SEQ ID NO: 570) T39971_T12 1869 1885 (SEQ ID NO: 571) T39971_T5 2412 2428 (SEQ ID NO: 573)

Segment cluster T39971_node_(—)36 (SEQ ID NO: 597) according to the present invention is supported by 51 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): T39971_T10 (SEQ ID NO: 570), T39971_T12 (SEQ ID NO: 571) and T39971_T5 (SEQ ID NO: 573). Table 38 below describes the starting and ending position of this segment on each transcript.

TABLE 38 Segment location on transcripts Segment Segment Transcript name starting position ending position T39971_T10 2138 2199 (SEQ ID NO: 570) T39971_T12 1886 1947 (SEQ ID NO: 571) T39971_T5 2429 2490 (SEQ ID NO: 573)

Segment cluster T39971_node_(—)4 (SEQ ID NO: 598) according to the present invention can be found in the following transcript(s): T39971_T10 (SEQ ID NO: 570), T39971_T12 (SEQ ID NO: 571), T39971_T16 (SEQ ID NO: 572) and T39971_T5 (SEQ ID NO: 573). Table 39 below describes the starting and ending position of this segment on each transcript.

TABLE 39 Segment location on transcripts Segment Segment Transcript name starting position ending position T39971_T10 862 881 (SEQ ID NO: 570) T39971_T12 862 881 (SEQ ID NO: 571) T39971_T16 862 881 (SEQ ID NO: 572) T39971_T5 862 881 (SEQ ID NO: 573)

Segment cluster T39971_node_(—)5 (SEQ ID NO: 595) according to the present invention is supported by 80 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): T39971_T10 (SEQ ID NO: 570), T39971_T12 (SEQ ID NO: 571), T39971_T16 (SEQ ID NO: 572) and T39971_T5 (SEQ ID NO: 573). Table 40 below describes the starting and ending position of this segment on each transcript.

TABLE 40 Segment location on transcripts Segment Segment Transcript name starting position ending position T39971_T10 882 939 (SEQ ID NO: 570) T39971_T12 882 939 (SEQ ID NO: 571) T39971_T16 882 939 (SEQ ID NO: 572) T39971_T5 882 939 (SEQ ID NO: 573)

Segment cluster T39971_node_(—)8 (SEQ ID NO: 600) according to the present invention can be found in the following transcript(s): T39971_T10 (SEQ ID NO: 570), T39971_T12 (SEQ ID NO: 571), T39971_T16 (SEQ ID NO: 572) and T39971_T5 (SEQ ID NO: 573). Table 41 below describes the starting and ending position of this segment on each transcript.

TABLE 41 Segment location on transcripts Segment Segment Transcript name starting position ending position T39971_T10 1163 1168 (SEQ ID NO: 570) T39971_T12 1163 1168 (SEQ ID NO: 571) T39971_T16 1163 1168 (SEQ ID NO: 572) T39971_T5 1163 1168 (SEQ ID NO: 573)

Segment cluster T39971_node_(—)9 (SEQ ID NO: 601) according to the present invention can be found in the following transcript(s): T39971_T10 (SEQ ID NO: 570), T39971_T12 (SEQ ID NO: 571), T39971_T16 (SEQ ID NO: 572) and T39971_T5 (SEQ ID NO: 573). Table 42 below describes the starting and ending position of this segment on each transcript.

TABLE 42 Segment location on transcripts Segment Segment Transcript name starting position ending position T39971_T10 1169 1188 (SEQ ID NO: 570) T39971_T12 1169 1188 (SEQ ID NO: 571) T39971_T16 1169 1188 (SEQ ID NO: 572) T39971_T5 1169 1188 (SEQ ID NO: 573) Variant protein alignment to the previously known protein: Sequence name: /tmp/xkraCL2OcZ/43L7YcPH7x:VTNC_HUMAN Sequence documentation:

Alignment of: T39971_P6 (SEQ ID NO: 603) x VTNC_HUMAN ..

Alignment segment 1/1:

Quality: 2774.00 Escore: 0 Matching length: 278 Total length: 278 Matching Percent Similarity: 99.64 Matching Percent Identity: 99.64 Total Percent Similarity: 99.64 Total Percent Identity: 99.64 Gaps: 0

Alignment:

Sequence name: /tmp/X4DeeuSlB4/yMubSR5FPs:VTNC_HUMAN Sequence documentation:

Alignment of: T39971_P9 (SEQ ID NO: 604) x VTNC_HUMAN ..

Alignment segment 1/1:

Quality: 4430.00 Escore: 0 Matching length: 447 Total length: 478 Matching Percent Similarity: 100.00 Matching Percent Identity: 100.00 Total Percent Similarity: 93.51 Total Percent Identity: 93.51 Gaps: 1

Alignment:

Sequence name: /tmp/jvp1VtnxNy/wxNSeFVZZw:VTNC_HUMAN Sequence documentation:

Alignment of: T39971_P11 (SEQ ID NO: 605) x VTNC_HUMAN ..

Alignment segment 1/1:

Quality: 3576.00 Escore: 0 Matching length: 363 Total length: 478 Matching Percent Similarity: 100.00 Matching Percent Identity: 100.00 Total Percent Similarity: 75.94 Total Percent Identity: 75.94 Gaps: 1

Alignment:

Sequence name: /tmp/jvp1VtnxNy/wxNSeFVZZw:Q9BSH7 (SEQ ID NO: 1000) Sequence documentation:

Alignment of: T39971_P11 (SEQ ID NO: 605) x Q9BSH7 (SEQ ID NO: 1000) ..

Alignment segment 1/1:

Quality: 3576.00 Escore: 0 Matching length: 363 Total length: 478 Matching Percent Similarity: 100.00 Matching Percent Identity: 100.00 Total Percent Similarity: 75.94 Total Percent Identity: 75.94 Gaps: 1

Alignment:

Sequence name: /tmp/fgebv7ir41/48bTBMziJ0:VTNC_HUMAN Sequence documentation:

Alignment of: T39971_P12 (SEQ ID NO: 606) x VTNC_HUMAN ..

Alignment segment 1/1:

Quality: 2237.00 Escore: 0 Matching length: 223 Total length: 223 Matching Percent Similarity: 100.00 Matching Percent Identity: 100.00 Total Percent Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0

Alignment:

Sequence name: /tmp/fgebv7ir41/48bTBMziJ0:Q9BSH7 (SEQ ID NO: 1000) Sequence documentation:

Alignment of: T39971_P12 (SEQ ID NO: 606) x Q9BSH7 (SEQ ID NO: 1000) ..

Alignment segment 1/1:

Quality: 2237.00 Escore: 0 Matching length: 223 Total length: 223 Matching Percent Similarity: 100.00 Matching Percent Identity: 100.00 Total Percent Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0

Alignment:

Expression of VTNC_HUMAN Vitronectin (Serum Spreading Factor, Somatomedin B, Complement S-Protein), T39971 Transcripts, which are Detectable by Amplicon as Depicted in Sequence Name T39971 junc23-33 (SEQ ID NO: 1003) in Normal and Cancerous Ovary Tissues

Expression of VTNC_HUMAN vitronectin (serum spreading factor, somatomedin B, complement S-protein) transcripts detectable by or according to junc23-33, T39971 junc23-33 (SEQ ID NO:1003) amplicon(s) and T39971 junc23-33F (SEQ ID NO:1001) and T39971 junc23-33R (SEQ ID NO:1002) primers was measured by real time PCR. In parallel the expression of four housekeeping genes PBGD (GenBank Accession No. BC019323 (SEQ ID NO: 1036); amplicon—PBGD-amplicon (SEQ ID NO: 1039)), HPRT1 (GenBank Accession No. NM_(—)000194 (SEQ ID NO:1040); amplicon—HPRT1-amplicon (SEQ ID NO:1044)), SDHA (GenBank Accession No. NM_(—)004168 (SEQ ID NO:1032); amplicon—SDHA-amplicon (SEQ ID NO:1035)), and GAPDH (GenBank Accession No. BC026907; GAPDH amplicon (SEQ ID NO: 1047)) was measured similarly. For each RT sample, the expression of the above amplicon was normalized to the geometric mean of the quantities of the housekeeping genes. The normalized quantity of each RT sample was then divided by the median of the quantities of the normal post-mortem (PM) samples (Sample Nos. 45-48, Table 1, above, “Tissue samples in testing panel”), to obtain a value of fold differential expression for each sample relative to median of the normal PM samples.

FIG. 30 is a histogram showing down regulation of the above-indicated VTNC_HUMAN vitronectin (serum spreading factor, somatomedin B, complement S-protein), transcripts in cancerous ovary samples relative to the normal samples.

As is evident from FIG. 30, the expression of VTNC_HUMAN vitronectin (serum spreading factor, somatomedin B, complement S-protein), transcripts detectable by the above amplicon(s) in most cancer samples was significantly lower than in the non-cancerous samples (Sample Nos. 45-48 Table 1, above, “Tissue samples in testing panel”).

Primer pairs are also optionally and preferably encompassed within the present invention; for example, for the above experiment, the following primer pair was used as a non-limiting illustrative example only of a suitable primer pair: T39971 junc23-33F (SEQ ID NO:1001) forward primer; and T39971 junc23-33R (SEQ ID NO:1002) reverse primer.

The present invention also preferably encompasses any amplicon obtained through the use of any suitable primer pair; for example, for the above experiment, the following amplicon was obtained as a non-limiting illustrative example only of a suitable amplicon: T39971 junc23-33 (SEQ ID NO:1003).

T39971 junc23-33 Forward primer (SEQ ID NO: 1001): GGGGCAGAACCTCTGACAAG T39971 junc23-33 Reverse primer (SEQ ID NO: 1002): GGGCAGCCCAGCCAGTA T39971 junc23-33 Amplicon (SEQ ID NO: 1003): GGGGCAGAACCTCTGACAAGTACTACCGAGTCAATCTTCGCACACGGCGA GTGGACACTGTGGACCCTCCCTACCCACGCTCCATCGCTCAGTACTGGCT GGGCTGCCC

Expression of VTNC_HUMAN Vitronectin (Serum Spreading Factor, Somatomedin B, Complement S-Protein), T39971 Transcripts, which are Detectable by Amplicon as Depicted in Sequence Name T39971junc23-33 (SEQ ID NO: 1003) in Different Normal Tissues

Expression of VTNC_HUMAN vitronectin (serum spreading factor, somatomedin B, complement S-protein) transcripts detectable by or according to T39971junc23-33 (SEQ ID NO: 1003) amplicon and T39971junc23-33F (SEQ ID NO: 1001) and T39971junc23-33R (SEQ ID NO: 1002) was measured by real time PCR. In parallel the expression of four housekeeping genes -RPL19 (Genbank Accession No. NM_(—)000981 (SEQ ID NO:1048); RPL19 amplicon (SEQ ID NO:1051)), TATA box (GenBank Accession No. NM_(—)003194 (SEQ ID NO:1052); TATA amplicon (SEQ ID NO:1055)), Ubiquitin (GenBank Accession No. BC000449 (SEQ ID NO: 1056); amplicon—Ubiquitin-amplicon (SEQ ID NO: 1059)) and SDHA (GenBank Accession No. NM_(—)004168 (SEQ ID NO:1032); amplicon—SDHA-amplicon (SEQ ID NO: 1035)) was measured similarly. For each RT sample, the expression of the above amplicon was normalized to the geometric mean of the quantities of the housekeeping genes. The normalized quantity of each RT sample was then divided by the median of the quantities of the breast samples (Sample Nos. 33-35, Table 2 “Tissue samples in normal panel” above), to obtain a value of relative expression of each sample relative to median of the breast samples.

The results are described in FIG. 31, presenting the histogram showing the expression of T39971 transcripts, which are detectable by amplicon as depicted in sequence name T39971junc23-33 (SEQ ID NO:1003), in different normal tissues. Primers and amplicon are as above.

Description for Cluster Z44808

Cluster Z44808 features 5 transcript(s) and 21 segment(s) of interest, the names for which are given in Tables 1 and 2, respectively, the sequences themselves are given at the end of the application. The selected protein variants are given in table 3.

TABLE 1 Transcripts of interest Transcript Name SEQ ID NO: Z44808_PEA_1_T11 607 Z44808_PEA_1_T4 608 Z44808_PEA_1_T5 609 Z44808_PEA_1_T8 610 Z44808_PEA_1_T9 611

TABLE 2 Segments of interest Segment Name SEQ ID NO: Z44808_PEA_1_node_0 612 Z44808_PEA_1_node_16 613 Z44808_PEA_1_node_2 614 Z44808_PEA_1_node_24 615 Z44808_PEA_1_node_32 616 Z44808_PEA_1_node_33 617 Z44808_PEA_1_node_36 618 Z44808_PEA_1_node_37 619 Z44808_PEA_1_node_41 620 Z44808_PEA_1_node_11 621 Z44808_PEA_1_node_13 622 Z44808_PEA_1_node_18 623 Z44808_PEA_1_node_22 624 Z44808_PEA_1_node_26 625 Z44808_PEA_1_node_30 626 Z44808_PEA_1_node_34 627 Z44808_PEA_1_node_35 628 Z44808_PEA_1_node_39 629 Z44808_PEA_1_node_4 630 Z44808_PEA_1_node_6 631 Z44808_PEA_1_node_8 632

TABLE 3 Proteins of interest Protein Name SEQ ID NO: Z44808_PEA_1_P5 634 Z44808_PEA_1_P6 635 Z44808_PEA_1_P7 636 Z44808_PEA_1_P11 637

These sequences are variants of the known protein SPARC related modular calcium-binding protein 2 precursor (SwissProt accession identifier SMO2_HUMAN; known also according to the synonyms Secreted modular calcium-binding protein 2; SMOC-2; Smooth muscle-associated protein 2; SMAP-2; MSTP117), SEQ ID NO: 633, referred to herein as the previously known protein.

The sequence for protein SPARC related modular calcium-binding protein 2 precursor is given at the end of the application, as “SPARC related modular calcium-binding protein 2 precursor amino acid sequence”. Known polymorphisms for this sequence are as shown in Table 4.

TABLE 4 Amino acid mutations for Known Protein SNP position(s) on amino acid sequence Comment 169-170 KT -> TR 212 S -> P 429-446 TPRGHAESTSNRQPRKQG -> RSKRNL 434 A -> V 439 N -> Y

Protein SPARC related modular calcium-binding protein 2 precursor localization is believed to be Secreted.

Cluster Z44808 can be used as a diagnostic marker according to overexpression of transcripts of this cluster in cancer. Expression of such transcripts in normal tissues is also given according to the previously described methods. The term “number” in the right hand column of the table and the numbers on the y-axis of FIG. 32 refer to weighted expression of ESTs in each category, as “parts per million” (ratio of the expression of ESTs for a particular cluster to the expression of all ESTs in that category, according to parts per million).

Overall, the following results were obtained as shown with regard to the histograms in FIG. 32 and Table 5. This cluster is overexpressed (at least at a minimum level) in the following pathological conditions: colorectal cancer, lung cancer and pancreas carcinoma.

TABLE 5 Normal tissue distribution Name of Tissue Number bladder 123 bone 304 brain 18 colon 0 epithelial 40 general 37 kidney 2 lung 0 breast 61 ovary 116 pancreas 0 prostate 128 stomach 36 uterus 195

TABLE 6 P values and ratios for expression in cancerous tissue Name of Tissue P1 P2 SP1 R3 SP2 R4 bladder 6.8e−01 7.6e−01 7.7e−01 0.8 9.1e−01 0.6 bone 7.0e−01 8.8e−01 9.9e−01 0.3 1 0.2 brain 6.8e−01 7.2e−01 3.0e−02 2.6 1.7e−01 1.6 colon 9.2e−03 1.3e−02 1.2e−01 3.6 1.6e−01 3.1 epithelial 2.1e−02 4.0e−01 1.0e−04 1.9 2.7e−01 1.0 general 2.6e−02 7.2e−01 4.9e−07 1.9 3.0e−01 1.0 kidney 7.3e−01 8.1e−01 1 1.0 1 1.0 lung 4.0e−03 1.8e−02 8.0e−04 12.2 2.1e−02 6.0 breast 4.8e−01 6.1e−01 9.8e−02 2.0 3.9e−01 1.2 ovary 8.1e−01 8.3e−01 9.1e−01 0.6 9.7e−01 0.5 pancreas 1.2e−01 2.1e−01 1.0e−03 6.5 5.9e−03 4.6 prostate 8.4e−01 8.9e−01 9.0e−01 0.6 9.8e−01 0.4 stomach 5.0e−01 8.7e−01 9.6e−04 1.5 1.9e−01 0.8 uterus 6.7e−01 7.9e−01 9.2e−01 0.5 1 0.3

As noted above, cluster Z44808 features 5 transcript(s), which were listed in Table 1 above. These transcript(s) encode for protein(s) which are variant(s) of protein SPARC related modular calcium-binding protein 2 precursor. A description of each variant protein according to the present invention is now provided.

Variant protein Z44808_PEA_(—)1_P5 (SEQ ID NO: 634) according to the present invention has an amino acid sequence as given at the end of the application; it is encoded by transcript(s) Z44808_PEA_(—)1_T4 (SEQ ID NO: 608). An alignment is given to the known protein (SPARC related modular calcium-binding protein 2 precursor) at the end of the application. One or more alignments to one or more previously published protein sequences are given at the end of the application. A brief description of the relationship of the variant protein according to the present invention to each such aligned protein is as follows:

Comparison report between Z44808_PEA_(—)1_P5 (SEQ ID NO: 634) and SMO2_HUMAN:

1. An isolated chimeric polypeptide encoding for Z44808_PEA_(—)1_P5 (SEQ ID NO: 634), comprising a first amino acid sequence being at least 90% homologous to MLLPQLCWLPLLAGLLPPVPAQKFSALTFLRVDQDKDKDCSLDCAGSPQKPLCASDGR TFLSRCEFQRAKCKDPQLEIAYRGNCKDVSRCVAERKYTQEQARKEFQQVFIPECNDD GTYSQVQCHSYTGYCWCVTPNGRPISGTAVAHKTPRCPGSVNEKLPQREGTGKTDDAA APALETQPQGDEEDIASRYPTLWTEQVKSRQNKTNKNSVSSCDQEHQSALEEAKQPKN DNVVIPECAHGGLYKPVQCHPSTGYCWCVLVDTGRPIPGTSTRYEQPKCDNTARAHPA KARDLYKGRQLQGCPGAKKHEFLTSVLDALSTDMVHAASDPSSSSGRLSEPDPSHTLEE RVVHWYFKLLDKNSSGDIGKKEIKPFKRFLRKKSKPKKCVKKFVEYCDVNNDKSISVQ ELMGCLGVAKEDGKADTKKRHTPRGHAESTSNRQ corresponding to amino acids 1-441 of SMO2_HUMAN, which also corresponds to amino acids 1-441 of Z44808_PEA_(—)1_P5 (SEQ ID NO: 634), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence DAMVVSSRPKATTHRKSRTLSRR (SEQ ID NO: 1077) corresponding to amino acids 442-464 of Z44808_PEA_(—)1_P5 (SEQ ID NO: 634), wherein said first and second amino acid sequences are contiguous and in a sequential order.

2. An isolated polypeptide encoding for a tail of Z44808_PEA_(—)1_P5 (SEQ ID NO: 634), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence DAMVVSSRPKATTHRKSRTLSRR (SEQ ID NO: 1077) in Z44808_PEA_(—)1_P5 (SEQ ID NO: 634).

The location of the variant protein was determined according to results from a number of different software programs and analyses, including analyses from SignalP and other specialized programs. The variant protein is believed to be located as follows with regard to the cell: secreted. The protein localization is believed to be secreted because both signal-peptide prediction programs predict that this protein has a signal peptide, and neither trans-membrane region prediction program predicts that this protein has a trans-membrane region.

Variant protein Z44808_PEA_(—)1_P5 (SEQ ID NO: 634) is encoded by the following transcript(s): Z44808_PEA_(—)1_T4 (SEQ ID NO: 608), for which the sequence(s) is/are given at the end of the application. The coding portion of transcript Z44808_PEA_(—)1_T4 (SEQ ID NO: 608) is shown in bold; this coding portion starts at position 586 and ends at position 1977. The transcript also has the following SNPs as listed in Table 7 (given according to their position on the nucleotide sequence, with the alternative nucleic acid listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein Z44808_PEA_(—)1_P5 (SEQ ID NO: 634) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 7 Nucleic acid SNPs SNP position on nucleotide sequence Alternative nucleic acid Previously known SNP? 549 A -> G No 648 T -> G No 4403 G -> T No 4456 G -> A Yes 4964 G -> C Yes 1025 C -> No 1677 T -> C No 2691 C -> T Yes 3900 T -> C No 3929 G -> A Yes 4099 G -> T Yes 4281 T -> C No 4319 G -> C Yes

Variant protein Z44808_PEA_(—)1_P6 (SEQ ID NO: 635) according to the present invention has an amino acid sequence as given at the end of the application; it is encoded by transcript(s) Z44808_PEA_(—)1_T5 (SEQ ID NO: 609). An alignment is given to the known protein (SPARC related modular calcium-binding protein 2 precursor) at the end of the application. One or more alignments to one or more previously published protein sequences are given at the end of the application. A brief description of the relationship of the variant protein according to the present invention to each such aligned protein is as follows:

Comparison report between Z44808_PEA_(—)1_P6 (SEQ ID NO: 635) and SMO2_HUMAN:

1. An isolated chimeric polypeptide encoding for Z44808_PEA_(—)1_P6 (SEQ ID NO: 635), comprising a first amino acid sequence being at least 90% homologous to MLLPQLCWLPLLAGLLPPVPAQKFSALTFLRVDQDKDKDCSLDCAGSPQKPLCASDGR TFLSRCEFQRAKCKDPQLEIAYRGNCKDVSRCVAERKYTQEQARKEFQQVFIPECNDD GTYSQVQCHSYTGYCWCVTPNGRPISGTAVAHKTPRCPGSVNEKLPQREGTGKTDDAA APALETQPQGDEEDIASRYPTLWTEQVKSRQNKTNKNSVSSCDQEHQSALEEAKQPKN DNVVIPECAHGGLYKPVQCHPSTGYCWCVLVDTGRPIPGTSTRYEQPKCDNTARAHPA KARDLYKGRQLQGCPGAKKHEFLTSVLDALSTDMVHAASDPSSSSGRLSEPDPSHTLEE RVVHWYFKLLDKNSSGDIGKKEIKPFKRFLRKKSKPKKCVKKFVEYCDVNNDKSISVQ ELMGCLGVAKEDGKADTKKRH corresponding to amino acids 1-428 of SMO2_HUMAN, which also corresponds to amino acids 1-428 of Z44808_PEA_(—)1_P6 (SEQ ID NO: 635), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence RSKRNL (SEQ ID NO: 1078) corresponding to amino acids 429-434 of Z44808_PEA_(—)1_P6 (SEQ ID NO: 635), wherein said first and second amino acid sequences are contiguous and in a sequential order.

2. An isolated polypeptide encoding for a tail of Z44808_PEA_(—)1_P6 (SEQ ID NO: 635), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence RSKRNL (SEQ ID NO: 1078) in Z44808_PEA_(—)1_P6 (SEQ ID NO: 635).

The location of the variant protein was determined according to results from a number of different software programs and analyses, including analyses from SignalP and other specialized programs. The variant protein is believed to be located as follows with regard to the cell: secreted. The protein localization is believed to be secreted because both signal-peptide prediction programs predict that this protein has a signal peptide, and neither trans-membrane region prediction program predicts that this protein has a trans-membrane region.

Variant protein Z44808_PEA_(—)1_P6 (SEQ ID NO: 635) also has the following non-silent SNPs (Single Nucleotide Polymorphisms) as listed in Table 8, (given according to their position(s) on the amino acid sequence, with the alternative amino acid(s) listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein Z44808_PEA_(—)1_P6 (SEQ ID NO: 635) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 8 Amino acid mutations SNP position(s) on amino acid sequence Alternative amino acid(s) Previously known SNP? 147 A -> No

Variant protein Z44808_PEA_(—)1_P6 (SEQ ID NO: 635) is encoded by the following transcript(s): Z44808_PEA_(—)1_T5 (SEQ ID NO: 609), for which the sequence(s) is/are given at the end of the application. The coding portion of transcript Z44808_PEA_(—)1_T5 (SEQ ID NO: 609) is shown in bold; this coding portion starts at position 586 and ends at position 1887. The transcript also has the following SNPs as listed in Table 9 (given according to their position on the nucleotide sequence, with the alternative nucleic acid listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein Z44808_PEA_(—)1_P6 (SEQ ID NO: 635) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 9 Nucleic acid SNPs SNP position on nucleotide sequence Alternative nucleic acid Previously known SNP? 549 A -> G No 648 T -> G No 2866 G -> A Yes 3374 G -> C Yes 1025 C -> No 1677 T -> C No 2310 T -> C No 2339 G -> A Yes 2509 G -> T Yes 2691 T -> C No 2729 G -> C Yes 2813 G -> T No

Variant protein Z44808_PEA_(—)1_P7 (SEQ ID NO: 636) according to the present invention has an amino acid sequence as given at the end of the application; it is encoded by transcript(s) Z44808_PEA_(—)1_T9 (SEQ ID NO: 611). An alignment is given to the known protein (SPARC related modular calcium-binding protein 2 precursor) at the end of the application. One or more alignments to one or more previously published protein sequences are given at the end of the application. A brief description of the relationship of the variant protein according to the present invention to each such aligned protein is as follows:

Comparison report between Z44808_PEA_(—)1_P7 (SEQ ID NO: 636) and SMO2_HUMAN:

1. An isolated chimeric polypeptide encoding for Z44808_PEA_(—)1_P7 (SEQ ID NO: 636), comprising a first amino acid sequence being at least 90% homologous to MLLPQLCWLPLLAGLLPPVPAQKFSALTFLRVDQDKDKDCSLDCAGSPQKPLCASDGR TFLSRCEFQRAKCKDPQLEIAYRGNCKDVSRCVAERKYTQEQARKEFQQVFIPECNDD GTYSQVQCHSYTGYCWCVTPNGRPISGTAVAHKTPRCPGSVNEKLPQREGTGKTDDAA APALETQPQGDEEDIASRYPTLWTEQVKSRQNKTNKNSVSSCDQEHQSALEEAKQPKN DNVVIPECAHGGLYKPVQCHPSTGYCWCVLVDTGRPIPGTSTRYEQPKCDNTARAHPA KARDLYKGRQLQGCPGAKKHEFLTSVLDALSTDMVHAASDPSSSSGRLSEPDPSHTLEE RVVHWYFKLLDKNSSGDIGKKEIKPFKRFLRKKSKPKKCVKKFVEYCDVNNDKSISVQ ELMGCLGVAKEDGKADTKKRHTPRGHAESTSNRQ corresponding to amino acids 1-441 of SMO2_HUMAN, which also corresponds to amino acids 1-441 of Z44808_PEA_(—)1_P7 (SEQ ID NO: 636), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence LLWLRGKVSFYCF (SEQ ID NO: 1079) corresponding to amino acids 442-454 of Z44808_PEA_(—)1_P7 (SEQ ID NO: 636), wherein said first and second amino acid sequences are contiguous and in a sequential order.

2. An isolated polypeptide encoding for a tail of Z44808_PEA_(—)1_P7 (SEQ ID NO: 636), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence LLWLRGKVSFYCF (SEQ ID NO: 1079) in Z44808_PEA_(—)1_P7 (SEQ ID NO: 636).

The location of the variant protein was determined according to results from a number of different software programs and analyses, including analyses from SignalP and other specialized programs. The variant protein is believed to be located as follows with regard to the cell: secreted. The protein localization is believed to be secreted because both signal-peptide prediction programs predict that this protein has a signal peptide, and neither trans-membrane region prediction program predicts that this protein has a trans-membrane region.

Variant protein Z44808_PEA_(—)1_P7 (SEQ ID NO: 636) also has the following non-silent SNPs (Single Nucleotide Polymorphisms) as listed in Table 10, (given according to their position(s) on the amino acid sequence, with the alternative amino acid(s) listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein Z44808_PEA_(—)1_P7 (SEQ ID NO: 636) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 10 Amino acid mutations SNP position(s) on amino acid sequence Alternative amino acid(s) Previously known SNP? 147 A -> No

Variant protein Z44808_PEA_(—)1_P7 (SEQ ID NO: 636) is encoded by the following transcript(s): Z44808_PEA_(—)1_T9 (SEQ ID NO: 611), for which the sequence(s) is/are given at the end of the application. The coding portion of transcript Z44808_PEA_(—)1_T9 (SEQ ID NO: 611) is shown in bold; this coding portion starts at position 586 and ends at position 1947. The transcript also has the following SNPs as listed in Table 11 (given according to their position on the nucleotide sequence, with the alternative nucleic acid listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein Z44808_PEA_(—)1_P7 (SEQ ID NO: 636) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 11 Nucleic acid SNPs SNP position on nucleotide sequence Alternative nucleic acid Previously known SNP? 549 A -> G No 648 T -> G No 1025 C -> No 1677 T -> C No 2169 C -> A Yes

Variant protein Z44808_PEA_(—)1_P11 (SEQ ID NO: 637) according to the present invention has an amino acid sequence as given at the end of the application; it is encoded by transcript(s) Z44808_PEA_(—)1_T11 (SEQ ID NO: 607). The identification of this transcript was performed using a non-EST based method for identification of alternative splicing, described in the following reference: “Sorek R et al., Genome Res. (2004) 14:1617-23.” An alignment is given to the known protein (SPARC related modular calcium-binding protein 2 precursor) at the end of the application. One or more alignments to one or more previously published protein sequences are given at the end of the application. A brief description of the relationship of the variant protein according to the present invention to each such aligned protein is as follows:

Comparison report between Z44808_PEA_(—)1_P11 (SEQ ID NO: 637) and SMO2_HUMAN:

1. An isolated chimeric polypeptide encoding for Z44808_PEA_(—)1_P11 (SEQ ID NO: 637), comprising a first amino acid sequence being at least 90% homologous to MLLPQLCWLPLLAGLLPPVPAQKFSALTFLRVDQDKDKDCSLDCAGSPQKPLCASDGR TFLSRCEFQRAKCKDPQLEIAYRGNCKDVSRCVAERKYTQEQARKEFQQVFIPECNDD GTYSQVQCHSYTGYCWCVTPNGRPISGTAVAHKTPRCPGSVNEKLPQREGTGKT corresponding to amino acids 1-170 of SMO2_HUMAN, which also corresponds to amino acids 1-170 of Z44808_PEA_(—)1_P11 (SEQ ID NO: 637), and a second amino acid sequence being at least 90% homologous to DIASRYPTLWTEQVKSRQNKTNKNSVSSCDQEHQSALEEAKQPKNDNVVIPECAHGGL YKPVQCHPSTGYCWCVLVDTGRPIPGTSTRYEQPKCDNTARAHPAKARDLYKGRQLQ GCPGAKKHEFLTSVLDALSTDMVHAASDPSSSSGRLSEPDPSHTLEERVVHWYFKLLD KNSSGDIGKKEIKPFKRFLRKKSKPKKCVKKFVEYCDVNNDKSISVQELMGCLGVAKE DGKADTKKRHTPRGHAESTSNRQPRKQG corresponding to amino acids 188-446 of SMO2_HUMAN, which also corresponds to amino acids 171-429 of Z44808_PEA_(—)1_P11 (SEQ ID NO: 637), wherein said first and second amino acid sequences are contiguous and in a sequential order.

2. An isolated chimeric polypeptide encoding for an edge portion of Z44808_PEA_(—)1_P11 (SEQ ID NO: 637), comprising a polypeptide having a length “n”, wherein n is at least about 10 amino acids in length, optionally at least about 20 amino acids in length, preferably at least about 30 amino acids in length, more preferably at least about 40 amino acids in length and most preferably at least about 50 amino acids in length, wherein at least two amino acids comprise TD, having a structure as follows: a sequence starting from any of amino acid numbers 170-x to −170; and ending at any of amino acid numbers 171+((n−2)−x), in which x varies from 0 to n−2.

The location of the variant protein was determined according to results from a number of different software programs and analyses, including analyses from SignalP and other specialized programs. The variant protein is believed to be located as follows with regard to the cell: secreted. The protein localization is believed to be secreted because both signal-peptide prediction programs predict that this protein has a signal peptide, and neither trans-membrane region prediction program predicts that this protein has a trans-membrane region.

Variant protein Z44808_PEA_(—)1_P11 (SEQ ID NO: 637) also has the following non-silent SNPs (Single Nucleotide Polymorphisms) as listed in Table 12, (given according to their position(s) on the amino acid sequence, with the alternative amino acid(s) listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein Z44808_PEA_(—)1_P11 (SEQ ID NO: 637) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 12 Amino acid mutations SNP position(s) on amino acid sequence Alternative amino acid(s) Previously known SNP? 147 A -> No

Variant protein Z44808_PEA_(—)1_P11 (SEQ ID NO: 637) is encoded by the following transcript(s): Z44808_PEA_(—)1_T11 (SEQ ID NO: 607), for which the sequence(s) is/are given at the end of the application. The coding portion of transcript Z44808_PEA_(—)1_T11 (SEQ ID NO: 607) is shown in bold; this coding portion starts at position 586 and ends at position 1872. The transcript also has the following SNPs as listed in Table 13 (given according to their position on the nucleotide sequence, with the alternative nucleic acid listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein Z44808_PEA_(—)1_P11 (SEQ ID NO: 637) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 13 Nucleic acid SNPs SNP position on nucleotide sequence Alternative nucleic acid Previously known SNP? 549 A -> G No 648 T -> G No 2720 G -> A Yes 3228 G -> C Yes 1025 C -> No 1626 T -> C No 2164 T -> C No 2193 G -> A Yes 2363 G -> T Yes 2545 T -> C No 2583 G -> C Yes 2667 G -> T No

As noted above, cluster Z44808 features 21 segment(s), which were listed in Table 2 above and for which the sequence(s) are given at the end of the application. These segment(s) are portions of nucleic acid sequence(s) which are described herein separately because they are of particular interest. A description of each segment according to the present invention is now provided.

Segment cluster Z44808_PEA_(—)1_node_(—)0 (SEQ ID NO: 612) according to the present invention is supported by 29 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): Z44808_PEA_(—)1_T11 (SEQ ID NO: 607), Z44808_PEA_(—)1_T4 (SEQ ID NO: 608), Z44808_PEA_(—)1_T5 (SEQ ID NO: 609), Z44808_PEA_(—)1_T8 (SEQ ID NO: 610) and Z44808_PEA_(—)1_T9 (SEQ ID NO: 611). Table 14 below describes the starting and ending position of this segment on each transcript.

TABLE 14 Segment location on transcripts Segment Segment Transcript name starting position ending position Z44808_PEA_1_T11 1 669 (SEQ ID NO: 607) Z44808_PEA_1_T4 1 669 (SEQ ID NO: 608) Z44808_PEA_1_T5 1 669 (SEQ ID NO: 609) Z44808_PEA_1_T8 1 669 (SEQ ID NO: 610) Z44808_PEA_1_T9 1 669 (SEQ ID NO: 611)

Segment cluster Z44808_PEA_(—)1_node_(—)16 (SEQ ID NO: 613) according to the present invention is supported by 39 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): Z44808_PEA_(—)1_T11 (SEQ ID NO: 607), Z44808_PEA_(—)1_T4 (SEQ ID NO: 608), Z44808_PEA_(—)1_T5 (SEQ ID NO: 609), Z44808_PEA_(—)1_T8 (SEQ ID NO: 610) and Z44808_PEA_(—)1_T9 (SEQ ID NO: 611). Table 15 below describes the starting and ending position of this segment on each transcript.

TABLE 15 Segment location on transcripts Segment Segment Transcript name starting position ending position Z44808_PEA_1_T11 1172 1358 (SEQ ID NO: 607) Z44808_PEA_1_T4 1223 1409 (SEQ ID NO: 608) Z44808_PEA_I_T5 1223 1409 (SEQ ID NO: 609) Z44808_PEA_1_T8 1223 1409 (SEQ ID NO: 610) Z44808_PEA_1_T9 1223 1409 (SEQ ID NO: 611)

Segment cluster Z44808_PEA_(—)1_node_(—)2 (SEQ ID NO: 614) according to the present invention is supported by 34 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): Z44808_PEA_(—)1_T11 (SEQ ID NO: 607), Z44808_PEA_(—)1_T4 (SEQ ID NO: 608), Z44808_PEA_(—)1_T5 (SEQ ID NO: 609), Z44808_PEA_(—)1_T8 (SEQ ID NO: 610) and Z44808_PEA_(—)1_T9 (SEQ ID NO: 611). Table 16 below describes the starting and ending position of this segment on each transcript.

TABLE 16 Segment location on transcripts Segment Segment Transcript name starting position ending position Z44808_PEA_1_T11 670 841 (SEQ ID NO: 607) Z44808_PEA_1_T4 670 841 (SEQ ID NO: 608) Z44808_PEA_1_T5 670 841 (SEQ ID NO: 609) Z44808_PEA_1_T8 670 841 (SEQ ID NO: 610) Z44808_PEA_1_T9 670 841 (SEQ ID NO: 611)

Segment cluster Z44808_PEA_(—)1_node_(—)24 (SEQ ID NO: 615) according to the present invention is supported by 52 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): Z44808_PEA_(—)1_T11 (SEQ ID NO: 607), Z44808_PEA_(—)1_T4 (SEQ ID NO: 608), Z44808_PEA_(—)1_T5 (SEQ ID NO: 609), Z44808_PEA_(—)1_T8 (SEQ ID NO: 610) and Z44808_PEA_(—)1_T9 (SEQ ID NO: 611). Table 17 below describes the starting and ending position of this segment on each transcript.

TABLE 17 Segment location on transcripts Segment Segment Transcript name starting position ending position Z44808_PEA_1_T11 1545 1819 (SEQ ID NO: 607) Z44808_PEA_1_T4 1596 1870 (SEQ ID NO: 608) Z44808_PEA_1_T5 1596 1870 (SEQ ID NO: 609) Z44808_PEA_1_T8 1596 1870 (SEQ ID NO: 610) Z44808_PEA_1_T9 1596 1870 (SEQ ID NO: 611)

Segment cluster Z44808_PEA_(—)1_node_(—)32 (SEQ ID NO: 616) according to the present invention is supported by 17 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): Z44808_PEA_(—)1_T4 (SEQ ID NO: 608) and Z44808_PEA_(—)1_T8 (SEQ ID NO: 610). Table 18 below describes the starting and ending position of this segment on each transcript.

TABLE 18 Segment location on transcripts Segment Segment Transcript name starting position ending position Z44808_PEA_1_T4 1909 3593 (SEQ ID NO: 608) Z44808_PEA_1_T8 1909 2397 (SEQ ID NO: 610)

Segment cluster Z44808_PEA_(—)1_node_(—)33 (SEQ ID NO: 617) according to the present invention is supported by 133 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): Z44808_PEA_(—)1_T11 (SEQ ID NO: 607), Z44808_PEA_(—)1_T4 (SEQ ID NO: 608) and Z44808_PEA_(—)1_T5 (SEQ ID NO: 609). Table 20 below describes the starting and ending position of this segment on each transcript.

TABLE 20 Segment location on transcripts Segment Segment Transcript name starting position ending position Z44808_PEA_1_T11 1858 2734 (SEQ ID NO: 607) Z44808_PEA_1_T4 3594 4470 (SEQ ID NO: 608) Z44808_PEA_1_T5 2004 2880 (SEQ ID NO: 609)

Segment cluster Z44808_PEA_(—)1_node_(—)36 (SEQ ID NO: 618) according to the present invention is supported by 117 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): Z44808_PEA_(—)1_T11 (SEQ ID NO: 607), Z44808_PEA_(—)1_T4 (SEQ ID NO: 608) and Z44808_PEA_(—)1_T5 (SEQ ID NO: 609). Table 21 below describes the starting and ending position of this segment on each transcript.

TABLE 21 Segment location on transcripts Segment Segment Transcript name starting position ending position Z44808_PEA_1_T11 2829 3080 (SEQ ID NO: 607) Z44808_PEA_1_T4 4565 4816 (SEQ ID NO: 608) Z44808_PEA_1_T5 2975 3226 (SEQ ID NO: 609)

Segment cluster Z44808_PEA_(—)1_node_(—)37 (SEQ ID NO: 619) according to the present invention is supported by 120 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): Z44808_PEA_(—)1_T11 (SEQ ID NO: 607), Z44808_PEA_(—)1_T4 (SEQ ID NO: 608) and Z44808_PEA_(—)1_T5 (SEQ ID NO: 609). Table 22 below describes the starting and ending position of this segment on each transcript.

TABLE 22 Segment location on transcripts Segment Segment Transcript starting ending name position position Z44808_PEA_1_T11 3081 3429 (SEQ ID NO:607) Z44808_PEA_1_T4 4817 5165 (SEQ ID NO:608) Z44808_PEA_1_T5 3227 3575 (SEQ ID NO:609)

Segment cluster Z44808_PEA_(—)1_node_(—)4 (SEQ ID NO: 630) Z44808_PEA_(—)1_node_(—)4 (SEQ ID NO: 630)1 (SEQ ID NO: 620) according to the present invention is supported by 2 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): Z44808_PEA_(—)1_T9 (SEQ ID NO: 611). Table 23 below describes the starting and ending position of this segment on each transcript.

TABLE 23 Segment location on transcripts Segment Segment Transcript starting ending name position position Z44808_PEA_1_T9 1974 2206 (SEQ ID NO:611)

According to an optional embodiment of the present invention, short segments related to the above cluster are also provided. These segments are up to about 120 bp in length, and so are included in a separate description.

Segment cluster Z44808_PEA_(—)1_node_(—)11 (SEQ ID NO: 621) according to the present invention is supported by 25 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): Z44808_PEA_(—)1_T4 (SEQ ID NO: 608), Z44808_PEA_(—)1_T5 (SEQ ID NO: 609), Z44808_PEA_(—)1_T8 (SEQ ID NO: 610) and Z44808_PEA_(—)1_T9 (SEQ ID NO: 611). Table 24 below describes the starting and ending position of this segment on each transcript.

TABLE 24 Segment location on transcripts Segment Segment Transcript starting ending name position position Z44808_PEA_1_T4 1097 1147 (SEQ ID NO:608) Z44808_PEA_1_T5 1097 1147 (SEQ ID NO:609) Z44808_PEA_1_T8 1097 1147 (SEQ ID NO:610) Z44808_PEA_1_T9 1097 1147 (SEQ ID NO:611)

Segment cluster Z44808_PEA_(—)1_node_(—)13 (SEQ ID NO: 622) according to the present invention is supported by 28 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): Z44808_PEA_(—)1_T11 (SEQ ID NO: 607), Z44808_PEA_(—)1_T4 (SEQ ID NO: 608), Z44808_PEA_(—)1_T5 (SEQ ID NO: 609), Z44808_PEA_(—)1_T8 (SEQ ID NO: 610) and Z44808_PEA_(—)1_T9 (SEQ ID NO: 611). Table 25 below describes the starting and ending position of this segment on each transcript.

TABLE 25 Segment location on transcripts Segment Segment Transcript starting ending name position position Z44808_PEA_1_T11 1097 1171 (SEQ ID NO:607) Z44808_PEA_1_T4 1148 1222 (SEQ ID NO:608) Z44808_PEA_1_T5 1148 1222 (SEQ ID NO:609) Z44808_PEA_1_T8 1148 1222 (SEQ ID NO:610) Z44808_PEA_1_T9 1148 1222 (SEQ ID NO:611)

Segment cluster Z44808_PEA_(—)1_node_(—)18 (SEQ ID NO: 623) according to the present invention is supported by 27 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): Z44808_PEA_(—)1_T11 (SEQ ID NO: 607), Z44808_PEA_(—)1_T4 (SEQ ID NO: 608), Z44808_PEA_(—)1_T5 (SEQ ID NO: 609), Z44808_PEA_(—)1_T8 (SEQ ID NO: 610) and Z44808_PEA_(—)1_T9 (SEQ ID NO: 611). Table 26 below describes the starting and ending position of this segment on each transcript.

TABLE 26 Segment location on transcripts Segment Segment Transcript starting ending name position position Z44808_PEA_1_T11 1359 1441 (SEQ ID NO:607) Z44808_PEA_1_T4 1410 1492 (SEQ ID NO:608) Z44808_PEA_1_T5 1410 1492 (SEQ ID NO:609) Z44808_PEA_1_T8 1410 1492 (SEQ ID NO:610) Z44808_PEA_1_T9 1410 1492 (SEQ ID NO:611)

Segment cluster Z44808_PEA_(—)1_node_(—)22 (SEQ ID NO: 624) according to the present invention is supported by 33 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): Z44808_PEA_(—)1_T11 (SEQ ID NO: 607), Z44808_PEA_(—)1_T4 (SEQ ID NO: 608), Z44808_PEA_(—)1_T5 (SEQ ID NO: 609), Z44808_PEA_(—)1_T8 (SEQ ID NO: 610) and Z44808_PEA_(—)1_T9 (SEQ ID NO: 611). Table 27 below describes the starting and ending position of this segment on each transcript.

TABLE 27 Segment location on transcripts Segment Segment Transcript starting ending name position position Z44808_PEA_1_T11 1442 1544 (SEQ ID NO:607) Z44808_PEA_1_T4 1493 1595 (SEQ ID NO:608) Z44808_PEA_1_T5 1493 1595 (SEQ ID NO:609) Z44808_PEA_1_T8 1493 1595 (SEQ ID NO:610) Z44808_PEA_1_T9 1493 1595 (SEQ ID NO:611)

Segment cluster Z44808_PEA_(—)1_node_(—)26 (SEQ ID NO: 625) according to the present invention is supported by 2 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): Z44808_PEA_(—)1_T5 (SEQ ID NO: 609). Table 29 below describes the starting and ending position of this segment on each transcript.

TABLE 29 Segment location on transcripts Segment Segment Transcript starting ending name position position Z44808_PEA_1_T5 1871 1965 (SEQ ID NO:609)

Segment cluster Z44808_PEA_(—)1_node_(—)30 (SEQ ID NO: 626) according to the present invention is supported by 44 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): Z44808_PEA_(—)1_T11 (SEQ ID NO: 607), Z44808_PEA_(—)1_T4 (SEQ ID NO: 608), Z44808_PEA_(—)1_T5 (SEQ ID NO: 609), Z44808_PEA_(—)1_T8 (SEQ ID NO: 610) and Z44808_PEA_(—)1_T9 (SEQ ID NO: 611). Table 31 below describes the starting and ending position of this segment on each transcript.

TABLE 31 Segment location on transcripts Segment Segment Transcript starting ending name position position Z44808_PEA_1_T11 1820 1857 (SEQ ID NO:607) Z44808_PEA_1_T4 1871 1908 (SEQ ID NO:608) Z44808_PEA_1_T5 1966 2003 (SEQ ID NO:609) Z44808_PEA_1_T8 1871 1908 (SEQ ID NO:610) Z44808_PEA_1_T9 1871 1908 (SEQ ID NO:611)

Segment cluster Z44808_PEA_(—)1_node_(—)34 (SEQ ID NO: 627) according to the present invention is supported by 70 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): Z44808_PEA_(—)1_T11 (SEQ ID NO: 607), Z44808_PEA_(—)1_T4 (SEQ ID NO: 608) and Z44808_PEA_(—)1_T5 (SEQ ID NO: 609). Table 32 below describes the starting and ending position of this segment on each transcript.

TABLE 32 Segment location on transcripts Segment Segment Transcript starting ending name position position Z44808_PEA_1_T11 2735 2809 (SEQ ID NO:607) Z44808_PEA_1_T4 4471 4545 (SEQ ID NO:608) Z44808_PEA_1_T5 2881 2955 (SEQ ID NO:609)

Segment cluster Z44808_PEA_(—)1_node_(—)35 (SEQ ID NO: 628) according to the present invention can be found in the following transcript(s): Z44808_PEA_(—)1_T11 (SEQ ID NO: 607), Z44808_PEA_(—)1_T4 (SEQ ID NO: 608) and Z44808_PEA_(—)1_T5 (SEQ ID NO: 609). Table 33 below describes the starting and ending position of this segment on each transcript.

TABLE 33 Segment location on transcripts Segment Segment Transcript starting ending name position position Z44808_PEA_1_T11 2810 2828 (SEQ ID NO:607) Z44808_PEA_1_T4 4546 4564 (SEQ ID NO:608) Z44808_PEA_1_T5 2956 2974 (SEQ ID NO:609)

Segment cluster Z44808_PEA_(—)1_node_(—)39 (SEQ ID NO: 629) according to the present invention is supported by 1 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): Z44808_PEA_(—)1_T9 (SEQ ID NO: 611). Table 34 below describes the starting and ending position of this segment on each transcript.

TABLE 34 Segment location on transcripts Segment Segment Transcript starting ending name position position Z44808_PEA_1_T9 1909 1973 (SEQ ID NO:611)

Segment cluster Z44808_PEA_(—)1_node_(—)4 (SEQ ID NO: 630) according to the present invention is supported by 33 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): Z44808_PEA_(—)1_T11 (SEQ ID NO: 607), Z44808_PEA_(—)1_T4 (SEQ ID NO: 608), Z44808_PEA_(—)1_T5 (SEQ ID NO: 609), Z44808_PEA_(—)1_T8 (SEQ ID NO: 610) and Z44808_PEA_(—)1_T9 (SEQ ID NO: 611). Table 35 below describes the starting and ending position of this segment on each transcript.

TABLE 35 Segment location on transcripts Segment Segment Transcript starting ending name position position Z44808_PEA_1_T11 842 948 (SEQ ID NO:607) Z44808_PEA_1_T4 842 948 (SEQ ID NO:608) Z44808_PEA_1_T5 842 948 (SEQ ID NO:609) Z44808_PEA_1_T8 842 948 (SEQ ID NO:610) Z44808_PEA_1_T9 842 948 (SEQ ID NO:611)

Segment cluster Z44808_PEA_(—)1_node_(—)6 (SEQ ID NO: 631) according to the present invention is supported by 30 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): Z44808_PEA_(—)1_T11 (SEQ ID NO: 607), Z44808_PEA_(—)1_T4 (SEQ ID NO: 608), Z44808_PEA_(—)1_T5 (SEQ ID NO: 609), Z44808_PEA_(—)1_T8 (SEQ ID NO: 610) and Z44808_PEA_(—)1_T9 (SEQ ID NO: 611). Table 36 below describes the starting and ending position of this segment on each transcript.

TABLE 36 Segment location on transcripts Segment Segment Transcript starting ending name position position Z44808_PEA_1_T11 949 1048 (SEQ ID NO:607) Z44808_PEA_1_T4 949 1048 (SEQ ID NO:608) Z44808_PEA_1_T5 949 1048 (SEQ ID NO:609) Z44808_PEA_1_T8 949 1048 (SEQ ID NO:610) Z44808_PEA_1_T9 949 1048 (SEQ ID NO:611)

Segment cluster Z44808_PEA_(—)1_node_(—)8 (SEQ ID NO: 632) according to the present invention is supported by 25 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): Z44808_PEA_(—)1_T11 (SEQ ID NO: 607), Z44808_PEA_(—)1_T4 (SEQ ID NO: 608), Z44808_PEA_(—)1_T5 (SEQ ID NO: 609), Z44808_PEA_(—)1_T8 (SEQ ID NO: 610) and Z44808_PEA_(—)1_T9 (SEQ ID NO: 611). Table 37 below describes the starting and ending position of this segment on each transcript.

TABLE 37 Segment location on transcripts Segment Segment Transcript starting ending name position position Z44808_PEA_1_T11 1049 1096 (SEQ ID NO:607) Z44808_PEA_1_T4 1049 1096 (SEQ ID NO:608) Z44808_PEA_1_T5 1049 1096 (SEQ ID NO:609) Z44808_PEA_1_T8 1049 1096 (SEQ ID NO:610) Z44808_PEA_1_T9 1049 1096 (SEQ ID NO:611) Variant protein alignment to the previously known protein: Sequence name: /tmp/vUqLu6eAVZ/K3JDuPvaLo:SMO2_HUMAN Sequence documentation:

Alignment of: Z44808_PEA_(—)1_P5 (SEQ ID NO: 634) x SMO2_HUMAN ..

Alignment segment 1/1:

Quality: 4440.00 Escore: 0 Matching length: 441 Total length: 441 Matching Percent Similarity: 100.00 Matching Percent Identity: 100.00 Total Percent Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0

Alignment:

Sequence name: /tmp/QSUNfTsJ5y/kLOw5Vb6SD:SMO2_HUMAN Sequence documentation:

Alignment of: Z44808_PEA_(—)1_P6 (SEQ ID NO: 635) x SMO2_HUMAN ..

Alignment segment 1/1:

Quality: 4310.00 Escore: 0 Matching length: 428 Total length: 428 Matching Percent Similarity: 100.00 Matching Percent Identity: 100.00 Total Percent Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0

Alignment:

Sequence name: /tmp/MZVdR4PVdM/5uN8RwViJ1:SMO2_HUMAN Sequence documentation:

Alignment of: Z44808_PEA_(—)1_P7 (SEQ ID NO: 636) x SMO2 HUMAN ..

Alignment segment 1/1:

Quality: 4440.00 Escore: 0 Matching length: 441 Total length: 441 Matching Percent Similarity: 100.00 Matching Percent Identity: 100.00 Total Percent Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0

Alignment:

Sequence name: /tmp/3fGVxqLloe/J5mQduAd0F:SMO2_HUMAN Sequence documentation:

Alignment of: Z44808_PEA_(—)1_P11 (SEQ ID NO: 637) x SMO2_HUMAN ..

Alignment segment 1/1:

Quality: 4228.00 Escore: 0 Matching length: 429 Total length: 446 Matching Percent Similarity: 100.00 Matching Percent Identity: 100.00 Total Percent Similarity: 96.19 Total Percent Identity: 96.19 Gaps: 1

Alignment:

Expression of SMO2_HUMAN SPARC Related Modular Calcium-Binding Protein 2 Precursor (Secreted Modular Calcium-Binding Protein 2) (SMOC-2) (Smooth Muscle-Associated Protein 2) Z44808 Transcripts, which are Detectable by Amplicon as Depicted in Sequence Name Z44808 junc8-11 (SEQ ID NO: 1006) in Normal and Cancerous Ovary Tissues

Expression of SMO2_HUMAN SPARC related modular calcium-binding protein 2 precursor (Secreted modular calcium-binding protein 2) (SMOC-2) (Smooth muscle-associated protein 2) transcripts detectable by or according to junc8-11, Z44808 junc8-11 (SEQ ID NO: 1006) amplicon(s) and Z44808 junc8-11F (SEQ ID NO: 1004) and Z44808 junc8-11R (SEQ ID NO:1005) primers was measured by real time PCR. In parallel the expression of four housekeeping genes —PBGD (GenBank Accession No. BC019323 (SEQ ID NO:1036); amplicon—PBGD-amplicon (SEQ ID NO:1039)), HPRT1 (GenBank Accession No. NM_(—)000194 (SEQ ID NO: 1040); amplicon—HPRT1-amplicon (SEQ ID NO: 1044)), SDHA (GenBank Accession No. NM_(—)004168 (SEQ ID NO: 1032); amplicon—SDHA-amplicon (SEQ ID NO:1035)), and GAPDH (GenBank Accession No. BC026907; GAPDH amplicon (SEQ ID NO: 1047)) was measured similarly. For each RT sample, the expression of the above amplicon was normalized to the geometric mean of the quantities of the housekeeping genes. The normalized quantity of each RT sample was then divided by the median of the quantities of the normal post-mortem (PM) samples (Sample Nos. 45-48, 71, Table 1, “Tissue sample in testing panel”, above). The reciprocal of this ratio was then calculated, to obtain a value of fold down-regulation for each sample relative to median of the normal PM samples.

FIG. 33A is a histogram showing down regulation of the above-indicated SMO2_HUMAN SPARC related modular calcium-binding protein 2 precursor transcripts in cancerous ovary samples relative to the normal samples.

As is evident from FIG. 33A, the expression of SMO2_HUMAN SPARC related modular calcium-binding protein 2 precursor transcripts detectable by the above amplicon(s) in cancer samples was significantly lower than in the non-cancerous samples (Sample Nos. 45-48, 71, Table 1, “Tissue sample in testing panel”). Notably down regulation of at least 5 fold was found in 33 out of 43 adenocarcinoma samples.

Statistical analysis was applied to verify the significance of these results, as described below.

The P value for the difference in the expression levels of SMO2_HUMAN SPARC related modular calcium-binding protein 2 precursor transcripts detectable by the above amplicon(s) in ovary cancer samples versus the normal tissue samples was determined by T test as 4.47E-05. Threshold of 5 fold down regulation was found to differentiate between cancer and normal samples with P value of 1.75E-03 as checked by exact fisher test. The above values demonstrate statistical significance of the results.

Primer pairs are also optionally and preferably encompassed within the present invention; for example, for the above experiment, the following primer pair was used as a non-limiting illustrative example only of a suitable primer pair: Z44808 junc8-1° F. (SEQ ID NO:10046) forward primer; and Z44808 junc8-11R (SEQ ID NO:1005) reverse primer.

The present invention also preferably encompasses any amplicon obtained through the use of any suitable primer pair; for example, for the above experiment, the following amplicon was obtained as a non-limiting illustrative example only of a suitable amplicon: Z44808 junc8-11 (SEQ ID NO: 1006).

Z44808 junc8-11 Forward primer (SEQ ID NO:1004): GAAGGCACAGGAAAAACAGATATTG Z44808 junc8-11 Reverse primer (SEQ ID NO:1005): TGGTGCTCTTGGTCACAGGAT Z44808 junc8-11 Amplicon (SEQ ID NO:1006): GAAGGCACAGGAAAAACAGATATTGCATCACGTTACCCTACCCTTTGGAC TGAACAGGTTAAAAGTCGGCAGAACAAAACCAATAAGAATTCAGTGTCAT CCTGTGACCAAGAGCACCA

Expression of SMO2_HUMAN SPARC Related Modular Calcium-Binding Protein 2 Precursor (Secreted Modular Calcium-Binding Protein 2) (SMOC-2) (Smooth Muscle-Associated Protein 2) Z44808 Transcripts which are Detectable by Amplicon as Depicted in Sequence Name Z44808 junc8-11 (SEQ ID NO:1006) in Different Normal Tissues

Expression of SMO2_HUMAN SPARC related modular calcium-binding protein 2 precursor (Secreted modular calcium-binding protein 2) (SMOC-2) (Smooth muscle-associated protein 2) transcripts detectable by or according to Z44808junc8-11 (SEQ ID NO: 1006) amplicon(s) and primers: Z44808junc8-11F (SEQ ID NO: 1004) Z44808junc8-11R (SEQ ID NO: 1005) was measured by real time PCR. In parallel the expression of four housekeeping genes -RPL19 (GenBank Accession No. NM_(—)000981 (SEQ ID NO:1048); RPL19 amplicon (SEQ ID NO: 1051)), TATA box (GenBank Accession No. NM_(—)003194 (SEQ ID NO: 1052); TATA amplicon (SEQ ID NO:1055)), Ubiquitin (GenBank Accession No. BC000449 (SEQ ID NO:1056); amplicon—Ubiquitin-amplicon (SEQ ID NO:1059)) and SDHA (GenBank Accession No. NM_(—)004168 (SEQ ID NO: 1032); amplicon—SDHA-amplicon (SEQ ID NO:1035)) was measured similarly. For each RT sample, the expression of the above amplicon was normalized to the geometric mean of the quantities of the housekeeping genes. The normalized quantity of each RT sample was then divided by the median of the quantities of the ovary samples (Sample Nos. 18-20, Table 2: Tissue samples in normal panel, above), to obtain a value of relative expression of each sample relative to median of the ovary samples. Results are shown in FIG. 33B. Primers and amplicon are as above.

Description for Cluster S67314

Cluster S67314 features 4 transcript(s) and 8 segment(s) of interest, the names for which are given in Tables 1 and 2, respectively, the sequences themselves are given at the end of the application. The selected protein variants are given in table 3.

TABLE 1 Transcripts of interest Transcript Name SEQ ID NO: S67314_PEA_1_T4 638 S67314_PEA_1_T5 639 S67314_PEA_1_T6 640 S67314_PEA_1_T7 641

TABLE 2 Segments of interest Segment Name SEQ ID NO: S67314_PEA_1_node_0 642 S67314_PEA_1_node_11 643 S67314_PEA_1_node_13 644 S67314_PEA_1_node_15 645 S67314_PEA_1_node_17 646 S67314_PEA_1_node_4 647 S67314_PEA_1_node_10 648 S67314_PEA_1_node_3 649

TABLE 3 Proteins of interest Protein Name SEQ ID NO: S67314_PEA_1_P4 651 S67314_PEA_1_P5 652 S67314_PEA_1_P6 653 S67314_PEA_1_P7 654

These sequences are variants of the known protein Fatty acid-binding protein, heart (SwissProt accession identifier FABH_HUMAN; known also according to the synonyms H-FABP; Muscle fatty acid-binding protein; M-FABP; Mammary-derived growth inhibitor; MDGI), SEQ ID NO: 650, referred to herein as the previously known protein.

Protein Fatty acid-binding protein is known or believed to have the following function(s): FABP are thought to play a role in the intracellular transport of long-chain fatty acids and their acyl-CoA esters. The sequence for protein Fatty acid-binding protein is given at the end of the application, as “Fatty acid-binding protein, heart amino acid sequence”. Known polymorphisms for this sequence are as shown in Table 4.

TABLE 4 Amino acid mutations for Known Protein SNP position(s) on amino acid sequence Comment 1 V -> A 104 L -> K 124 C -> S 129 E -> Q

Protein Fatty acid-binding protein localization is believed to be cytoplasmic.

The following GO Annotation(s) apply to the previously known protein. The following annotation(s) were found: negative control of cell proliferation, which are annotation(s) related to Biological Process; and lipid binding, which are annotation(s) related to Molecular Function.

The GO assignment relies on information from one or more of the SwissProt/TremBl Protein knowledgebase, available from <http://www.expasy.ch/sprot/>; or Locuslink, available from <http://www.ncbi.nlm.nih.gov/projects/LocusLink/>.

As noted above, cluster S67314 features 4 transcript(s), which were listed in Table 1 above. These transcript(s) encode for protein(s) which are variant(s) of protein Fatty acid-binding protein. A description of each variant protein according to the present invention is now provided.

Variant protein S67314_PEA_(—)1_P4 (SEQ ID NO: 651) according to the present invention has an amino acid sequence as given at the end of the application; it is encoded by transcript(s) S67314_PEA_(—)1_T4 (SEQ ID NO: 638). An alignment is given to the known protein (Fatty acid-binding protein) at the end of the application. One or more alignments to one or more previously published protein sequences are given at the end of the application. A brief description of the relationship of the variant protein according to the present invention to each such aligned protein is as follows:

Comparison report between S67314_PEA_(—)1_P4 (SEQ ID NO: 651) and FABH_HUMAN:

1. An isolated chimeric polypeptide encoding for S67314_PEA_(—)1_P4 (SEQ ID NO: 651), comprising a first amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence MVDAFLGTWKLVDSKNFDDYMKSLGVGFATRQVASMTKPTTIIEKNGDILTLKTHSTF KNTEISFKLGVEFDETTADDRKVKSIVTLDGGKLVHLQKWDGQETTLVRELIDGKLIL corresponding to amino acids 1-116 of FABH_HUMAN, which also corresponds to amino acids 1-116 of S67314_PEA_(—)1_P4 (SEQ ID NO: 651), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence VRWATLELYLIGYYYCSFSQACSKKPSPPLRAVEAGTREWLWVRVVSGGNFLCSGFGL TQAGTQILPYRLHDCGQITFSKCNCKTGINNTNLVGLLGSL (SEQ ID NO: 1080) corresponding to amino acids 117-215 of S67314_PEA_(—)1_P4 (SEQ ID NO: 651), wherein said first and second amino acid sequences are contiguous and in a sequential order.

2. An isolated polypeptide encoding for a tail of S67314_PEA_(—)1_P4 (SEQ ID NO: 651), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence VRWATLELYLIGYYYCSFSQACSKKPSPPLRAVEAGTREWLWVRVVSGGNFLCSGFGL TQAGTQILPYRLHDCGQITFSKCNCKTGINNTNLVGLLGSL (SEQ ID NO: 1080) in S67314_PEA_(—)1_P4 (SEQ ID NO: 651).

Comparison report between S67314_PEA_(—)1_P4 (SEQ ID NO: 651) and AAP35373 (SEQ ID NO: 1007):

1. An isolated chimeric polypeptide encoding for S67314_PEA_(—)1_P4 (SEQ ID NO: 651), comprising a first amino acid sequence being at least 90% homologous to MVDAFLGTWKLVDSKNFDDYMKSLGVGFATRQVASMTKPTTIIEKNGDILTLKTHSTF KNTEISFKLGVEFDETTADDRKVKSIVTLDGGKLVHLQKWDGQETTLVRELIDGKLIL corresponding to amino acids 1-116 of AAP35373, which also corresponds to amino acids 1-116 of S67314_PEA_(—)1_P4 (SEQ ID NO: 651), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence VRWATLELYLIGYYYCSFSQACSKKPSPPLRAVEAGTREWLWVRVVSGGNFLCSGFGL TQAGTQILPYRLHDCGQITFSKCNCKTGINNTNLVGLLGSL (SEQ ID NO: 1080) corresponding to amino acids 117-215 of S67314_PEA_(—)1_P4 (SEQ ID NO: 651), wherein said first and second amino acid sequences are contiguous and in a sequential order.

2. An isolated polypeptide encoding for a tail of S67314_PEA_(—)1_P4 (SEQ ID NO: 651), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence VRWATLELYLIGYYYCSFSQACSKKPSPPLRAVEAGTREWLWVRVVSGGNFLCSGFGL TQAGTQILPYRLHDCGQITFSKCNCKTGINNTNLVGLLGSL (SEQ ID NO: 1080) in S67314_PEA_(—)1_P4 (SEQ ID NO: 651).

The location of the variant protein was determined according to results from a number of different software programs and analyses, including analyses from SignalP and other specialized programs. The variant protein is believed to be located as follows with regard to the cell: intracellularly. The protein localization is believed to be intracellular because neither of the trans-membrane region prediction programs predicted a trans-membrane region for this protein. In addition both signal-peptide prediction programs predict that this protein is a non-secreted protein.

Variant protein S67314_PEA_(—)1_P4 (SEQ ID NO: 651) also has the following non-silent SNPs (Single Nucleotide Polymorphisms) as listed in Table 5, (given according to their position(s) on the amino acid sequence, with the alternative amino acid(s) listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein S67314_PEA_(—)1_P4 (SEQ ID NO: 651) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 5 Amino acid mutations SNP position(s) on amino acid sequence Alternative amino acid(s) Previously known SNP? 53 K -> R Yes

Variant protein S67314_PEA_(—)1_P4 (SEQ ID NO: 651) is encoded by the following transcript(s): S67314_PEA_(—)1_T4 (SEQ ID NO: 638), for which the sequence(s) is/are given at the end of the application. The coding portion of transcript S67314_PEA_(—)1_T4 (SEQ ID NO: 638) is shown in bold; this coding portion starts at position 925 and ends at position 1569. The transcript also has the following SNPs as listed in Table 6 (given according to their position on the nucleotide sequence, with the alternative nucleic acid listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein S67314_PEA_(—)1_P4 (SEQ ID NO: 651) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 6 Nucleic acid SNPs SNP position on nucleotide sequence Alternative nucleic acid Previously known SNP? 580 T -> C Yes 1082 A -> G Yes 1670 A -> C Yes

Variant protein S67314_PEA_(—)1_P5 (SEQ ID NO: 652) according to the present invention has an amino acid sequence as given at the end of the application; it is encoded by transcript(s) S67314_PEA_(—)1_T5 (SEQ ID NO: 639). An alignment is given to the known protein (Fatty acid-binding protein, heart) at the end of the application. One or more alignments to one or more previously published protein sequences are given at the end of the application. A brief description of the relationship of the variant protein according to the present invention to each such aligned protein is as follows:

Comparison report between S67314_PEA_(—)1_P5 (SEQ ID NO: 652) and FABH_HUMAN:

1. An isolated chimeric polypeptide encoding for S67314_PEA_(—)1_P5 (SEQ ID NO: 652), comprising a first amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence MVDAFLGTWKLVDSKNFDDYMKSLGVGFATRQVASMTKPTTIIEKNGDILTLKTHSTF KNTEISFKLGVEFDETTADDRKVKSIVTLDGGKLVHLQKWDGQETTLVRELIDGKLIL corresponding to amino acids 1-116 of FABH_HUMAN, which also corresponds to amino acids 1-116 of S67314_PEA_(—)1_P5 (SEQ ID NO: 652), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence DVLTAWPSIYRRQVKVLREDEITILPWHLQWSREKATKLLRPTLPSYNNHGWEELRVG KSIV (SEQ ID NO: 1081) corresponding to amino acids 117-178 of S67314_PEA_(—)1_P5 (SEQ ID NO: 652), wherein said first and second amino acid sequences are contiguous and in a sequential order.

2. An isolated polypeptide encoding for a tail of S67314_PEA_(—)1_P5 (SEQ ID NO: 652), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence DVLTAWPSIYRRQVKVLREDEITILPWHLQWSREKATKLLRPTLPSYNNHGWEELRVG KSIV (SEQ ID NO: 1081) in S67314_PEA_(—)1_P5 (SEQ ID NO: 652).

Comparison report between S67314_PEA_(—)1_P5 (SEQ ID NO: 652) and AAP35373:

1. An isolated chimeric polypeptide encoding for S67314_PEA_(—)1_P5 (SEQ ID NO: 652), comprising a first amino acid sequence being at least 90% homologous to MVDAFLGTWKLVDSKNFDDYMKSLGVGFATRQVASMTKPTTIIEKNGDILTLKTHSTF KNTEISFKLGVEFDETTADDRKVKSIVTLDGGKLVHLQKWDGQETTLVRELIDGKLIL corresponding to amino acids 1-116 of AAP35373, which also corresponds to amino acids 1-116 of S67314_PEA_(—)1_P5 (SEQ ID NO: 652), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence DVLTAWPSIYRRQVKVLREDEITILPWHLQWSREKATKLLRPTLPSYNNHGWEELRVG KSIV (SEQ ID NO: 1081) corresponding to amino acids 117-178 of S67314_PEA_(—)1_P5 (SEQ ID NO: 652), wherein said first and second amino acid sequences are contiguous and in a sequential order.

2. An isolated polypeptide encoding for a tail of S67314_PEA_(—)1_P5 (SEQ ID NO: 652), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence DVLTAWPSIYRRQVKVLREDEITILPWHLQWSREKATKLLRPTLPSYNNHGWEELRVG KSIV (SEQ ID NO: 1081) in S67314_PEA_(—)1_P5 (SEQ ID NO: 652).

The location of the variant protein was determined according to results from a number of different software programs and analyses, including analyses from SignalP and other specialized programs. The variant protein is believed to be located as follows with regard to the cell: intracellularly. The protein localization is believed to be intracellular because neither of the trans-membrane region prediction programs predicted a trans-membrane region for this protein. In addition both signal-peptide prediction programs predict that this protein is a non-secreted protein.

Variant protein S67314_PEA_(—)1_P5 (SEQ ID NO: 652) also has the following non-silent SNPs (Single Nucleotide Polymorphisms) as listed in Table 7, (given according to their position(s) on the amino acid sequence, with the alternative amino acid(s) listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein S67314_PEA_(—)1_P5 (SEQ ID NO: 652) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 7 Amino acid mutations SNP position(s) on amino acid sequence Alternative amino acid(s) Previously known SNP? 53 K -> R Yes

Variant protein S67314_PEA_(—)1_P5 (SEQ ID NO: 652) is encoded by the following transcript(s): S67314_PEA_(—)1_T5 (SEQ ID NO: 639), for which the sequence(s) is/are given at the end of the application. The coding portion of transcript S67314_PEA_(—)1_T5 (SEQ ID NO: 639) is shown in bold; this coding portion starts at position 925 and ends at position 1458. The transcript also has the following SNPs as listed in Table 8 (given according to their position on the nucleotide sequence, with the alternative nucleic acid listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein S67314_PEA_(—)1_P5 (SEQ ID NO: 652) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 8 Nucleic acid SNPs SNP position on nucleotide sequence Alternative nucleic acid Previously known SNP? 580 T -> C Yes 1082 A -> G Yes 1326 A -> G Yes

Variant protein S67314_PEA_(—)1_P6 (SEQ ID NO: 653) according to the present invention has an amino acid sequence as given at the end of the application; it is encoded by transcript(s) S67314_PEA_(—)1_T6 (SEQ ID NO: 640). An alignment is given to the known protein (Fatty acid-binding protein, heart) at the end of the application. One or more alignments to one or more previously published protein sequences are given at the end of the application. A brief description of the relationship of the variant protein according to the present invention to each such aligned protein is as follows:

Comparison report between S67314_PEA_(—)1_P6 (SEQ ID NO: 653) and FABH_HUMAN:

1. An isolated chimeric polypeptide encoding for S67314_PEA_(—)1_P6 (SEQ ID NO: 653), comprising a first amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence MVDAFLGTWKLVDSKNFDDYMKSLGVGFATRQVASMTKPTTIIEKNGDILTLKTHSTF KNTEISFKLGVEFDETTADDRKVKSIVTLDGGKLVHLQKWDGQETTLVRELIDGKLIL corresponding to amino acids 1-116 of FABH_HUMAN, which also corresponds to amino acids 1-116 of S67314_PEA_(—)1_P6 (SEQ ID NO: 653), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence MEKLQLRNVK (SEQ ID NO: 1082) corresponding to amino acids 117-126 of S67314_PEA_(—)1_P6 (SEQ ID NO: 653), wherein said first and second amino acid sequences are contiguous and in a sequential order.

2. An isolated polypeptide encoding for a tail of S67314_PEA_(—)1_P6 (SEQ ID NO: 653), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence MEKLQLRNVK (SEQ ID NO: 1082) in S67314_PEA_(—)1_P6 (SEQ ID NO: 653).

Comparison report between S67314_PEA_(—)1_P6 (SEQ ID NO: 653) and AAP35373:

1. An isolated chimeric polypeptide encoding for S67314_PEA_(—)1_P6 (SEQ ID NO: 653), comprising a first amino acid sequence being at least 90% homologous to MVDAFLGTWKLVDSKNFDDYMKSLGVGFATRQVASMTKPTTIIEKNGDILTLKTHSTF KNTEISFKLGVEFDETTADDRKVKSIVTLDGGKLVHLQKWDGQETTLVRELIDGKLIL corresponding to amino acids 1-116 of AAP35373, which also corresponds to amino acids 1-116 of S67314_PEA_(—)1_P6 (SEQ ID NO: 653), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence MEKLQLRNVK (SEQ ID NO: 1082) corresponding to amino acids 117-126 of S67314_PEA_(—)1_P6 (SEQ ID NO: 653), wherein said first and second amino acid sequences are contiguous and in a sequential order.

2. An isolated polypeptide encoding for a tail of S67314_PEA_(—)1_P6 (SEQ ID NO: 653), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence MEKLQLRNVK (SEQ ID NO: 1082) in S67314_PEA_(—)1_P6 (SEQ ID NO: 653).

The location of the variant protein was determined according to results from a number of different software programs and analyses, including analyses from SignalP and other specialized programs. The variant protein is believed to be located as follows with regard to the cell: intracellularly. The protein localization is believed to be intracellular because neither of the trans-membrane region prediction programs predicted a trans-membrane region for this protein. In addition both signal-peptide prediction programs predict that this protein is a non-secreted protein.

Variant protein S67314_PEA_(—)1_P6 (SEQ ID NO: 653) also has the following non-silent SNPs (Single Nucleotide Polymorphisms) as listed in Table 9, (given according to their position(s) on the amino acid sequence, with the alternative amino acid(s) listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein S67314_PEA_(—)1_P6 (SEQ ID NO: 653) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 9 Amino acid mutations SNP position(s) on amino acid sequence Alternative amino acid(s) Previously known SNP? 53 K -> R Yes

Variant protein S67314_PEA_(—)1_P6 (SEQ ID NO: 653) is encoded by the following transcript(s): S67314_PEA_(—)1_T6 (SEQ ID NO: 640), for which the sequence(s) is/are given at the end of the application. The coding portion of transcript S67314_PEA_(—)1_T6 (SEQ ID NO: 640) is shown in bold; this coding portion starts at position 925 and ends at position 1302. The transcript also has the following SNPs as listed in Table 10 (given according to their position on the nucleotide sequence, with the alternative nucleic acid listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein S67314_PEA_(—)1_P6 (SEQ ID NO: 653) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 10 Nucleic acid SNPs SNP position on nucleotide sequence Alternative nucleic acid Previously known SNP? 580 T -> C Yes 1082 A -> G Yes 1444 T -> C Yes

Variant protein S67314_PEA_(—)1_P7 (SEQ ID NO: 654) according to the present invention has an amino acid sequence as given at the end of the application; it is encoded by transcript(s) S67314_PEA_(—)1_T7 (SEQ ID NO: 641. An alignment is given to the known protein (Fatty acid-binding protein) at the end of the application. One or more alignments to one or more previously published protein sequences are given at the end of the application. A brief description of the relationship of the variant protein according to the present invention to each such aligned protein is as follows:

Comparison report between S67314_PEA_(—)1_P7 (SEQ ID NO: 654) and FABH_HUMAN:

1. An isolated chimeric polypeptide encoding for S67314_PEA_(—)1_P7 (SEQ ID NO: 654), comprising a first amino acid sequence being at least 90% homologous to MVDAFLGTWKLVDSKNFDDYMKSL corresponding to amino acids 1-24 of FABH_HUMAN, which also corresponds to amino acids 1-24 of S67314_PEA_(—)1_P7 (SEQ ID NO: 654), second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence AHILITFPLPS (SEQ ID NO: 1143) corresponding to amino acids 25-35 of S67314_PEA_(—)1_P7 (SEQ ID NO: 654), and a third amino acid sequence being at least 90% homologous to GVGFATRQVASMTKPTTIIEKNGDILTLKTHSTFKNTEISFKLGVEFDETTADDRKVKSI VTLDGGKLVHLQKWDGQETTLVRELIDGKLILTLTHGTAVCTRTYEKEA corresponding to amino acids 25-133 of FABH_HUMAN, which also corresponds to amino acids 36-144 of S67314_PEA_(—)1_P7 (SEQ ID NO: 654), wherein said first, second, third and fourth amino acid sequences are contiguous and in a sequential order.

2. An isolated polypeptide encoding for an edge portion of S67314_PEA_(—)1_P7 (SEQ ID NO: 654), comprising an amino acid sequence being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence encoding for AHILITFPLPS (SEQ ID NO: 1143), corresponding to S67314_PEA_(—)1_P7 (SEQ ID NO: 654).

Comparison report between S67314_PEA_(—)1_P7 (SEQ ID NO: 654) and AAP35373:

1. An isolated chimeric polypeptide encoding for S67314_PEA_(—)1_P7 (SEQ ID NO: 654), comprising a first amino acid sequence being at least 90% homologous to MVDAFLGTWKLVDSKNFDDYMKSL corresponding to amino acids 1-24 of AAP35373, which also corresponds to amino acids 1-24 of S67314_PEA_(—)1_P7 (SEQ ID NO: 654), second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence AHILITFPLPS (SEQ ID NO: 1143) corresponding to amino acids 25-35 of S67314_PEA_(—)1_P7 (SEQ ID NO: 654), and a third amino acid sequence being at least 90% homologous to GVGFATRQVASMTKPTTIIEKNGDILTLKTHSTFKNTEISFKLGVEFDETTADDRKVKSI VTLDGGKLVHLQKWDGQETTLVRELIDGKLILTLTHGTAVCTRTYEKEA corresponding to amino acids 25-133 of AAP35373, which also corresponds to amino acids 36-144 of S67314_PEA_(—)1_P7 (SEQ ID NO: 654), wherein said first, second and third amino acid sequences are contiguous and in a sequential order.

2. An isolated polypeptide encoding for an edge portion of S67314_PEA_(—)1_P7 (SEQ ID NO: 654), comprising an amino acid sequence being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence encoding for AHILITFPLPS (SEQ ID NO: 1143), corresponding to S67314_PEA_(—)1_P7 (SEQ ID NO: 654).

The location of the variant protein was determined according to results from a number of different software programs and analyses, including analyses from SignalP and other specialized programs. The variant protein is believed to be located as follows with regard to the cell: intracellularly. The protein localization is believed to be intracellular because neither of the trans-membrane region prediction programs predicted a trans-membrane region for this protein. In addition both signal-peptide prediction programs predict that this protein is a non-secreted protein.

Variant protein S67314_PEA_(—)1_P7 (SEQ ID NO: 654) also has the following non-silent SNPs (Single Nucleotide Polymorphisms) as listed in Table 11, (given according to their position(s) on the amino acid sequence, with the alternative amino acid(s) listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein S67314_PEA_(—)1_P7 (SEQ ID NO: 654) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 11 Amino acid mutations SNP position(s) on amino acid sequence Alternative amino acid(s) Previously known SNP? 64 K -> R Yes

Variant protein S67314_PEA_(—)1_P7 (SEQ ID NO: 654) is encoded by the following transcript(s): S67314_PEA_(—)1_T7 (SEQ ID NO: 641, for which the sequence(s) is/are given at the end of the application. The coding portion of transcript S67314_PEA_(—)1_T7 (SEQ ID NO: 641 is shown in bold; this coding portion starts at position 925 and ends at position 1356. The transcript also has the following SNPs as listed in Table 12 (given according to their position on the nucleotide sequence, with the alternative nucleic acid listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein S67314_PEA_(—)1_P7 (SEQ ID NO: 654) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 12 Nucleic acid SNPs SNP position on nucleotide sequence Alternative nucleic acid Previously known SNP? 580 T -> C Yes 1115 A -> G Yes 2772 G -> A Yes 2896 C -> A Yes 2918 G -> C Yes 3003 A -> G Yes 3074 T -> G Yes 1344 T -> C Yes 1522 -> T No 1540 -> A No 1540 -> T No 1578 G -> A Yes 1652 G -> A Yes 2263 G -> A Yes 2605 T -> C Yes

As noted above, cluster S67314 features 8 segment(s), which were listed in Table 2 above and for which the sequence(s) are given at the end of the application. These segment(s) are portions of nucleic acid sequence(s) which are described herein separately because they are of particular interest. A description of each segment according to the present invention is now provided.

Segment cluster S67314_PEA_(—)1_node_(—)0 (SEQ ID NO: 642) according to the present invention is supported by 90 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): S67314_PEA_(—)1_T4 (SEQ ID NO: 638), S67314_PEA_(—)1_T5 (SEQ ID NO: 639), S67314_PEA_(—)1_T6 (SEQ ID NO: 640) and S67314_PEA_(—)1_T7 (SEQ ID NO: 641. Table 13 below describes the starting and ending position of this segment on each transcript.

TABLE 13 Segment location on transcripts Segment Segment Transcript starting ending name position position S67314_PEA_1_T4 1 997 (SEQ ID NO:638) S67314_PEA_1_T5 1 997 (SEQ ID NO:639) S67314_PEA_1_T6 1 997 (SEQ ID NO:640) S67314_PEA_1_T7 1 997 (SEQ ID NO:641

Segment cluster S67314_PEA_(—)1_node_(—)11 (SEQ ID NO: 643) according to the present invention is supported by 6 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): S67314_PEA_(—)1_T4 (SEQ ID NO: 638). Table 14 below describes the starting and ending position of this segment on each transcript.

TABLE 14 Segment location on transcripts Segment Segment Transcript starting ending name position position S67314_PEA_1_T4 1273 2110 (SEQ ID NO:638)

Segment cluster S67314_PEA_(—)1_node_(—)13 (SEQ ID NO: 644) according to the present invention is supported by 76 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): S67314_PEA_(—)1_T7 (SEQ ID NO: 641. Table 15 below describes the starting and ending position of this segment on each transcript.

TABLE 15 Segment location on transcripts Segment Segment Transcript starting ending name position position S67314_PEA_1_T7 1306 3531 (SEQ ID NO:641

Segment cluster S67314_PEA_(—)1_node_(—)15 (SEQ ID NO: 645) according to the present invention is supported by 1 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): S67314_PEA_(—)1_T5 (SEQ ID NO: 639). Table 16 below describes the starting and ending position of this segment on each transcript.

TABLE 16 Segment location on transcripts Segment Segment Transcript starting ending name position position S67314_PEA_1_T5 1273 1733 (SEQ ID NO:639)

Segment cluster S67314_PEA_(—)1_node_(—)17 (SEQ ID NO: 646) according to the present invention is supported by 4 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): S67314_PEA_(—)1_T6 (SEQ ID NO: 640). Table 17 below describes the starting and ending position of this segment on each transcript.

TABLE 17 Segment location on transcripts Segment Segment Transcript starting ending name position position S67314_PEA_1_T6 1273 1822 (SEQ ID NO:640)

Microarray (chip) data is also available for this segment as follows. As described above with regard to the cluster itself, various oligonucleotides were tested for being differentially expressed in various disease conditions, particularly cancer. The following oligonucleotides were found to hit this segment (with regard to ovarian cancer), shown in Table 18.

TABLE 18 Oligonucleotides related to this segment Oligonucleotide Overexpressed Chip name in cancers reference S67314_0_0744 ovarian OVA (SEQ ID NO:1022) carcinoma

Segment cluster S67314_PEA_(—)1_node_(—)4 (SEQ ID NO: 647) according to the present invention is supported by 101 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): S67314_PEA_(—)1_T4 (SEQ ID NO: 638), S67314_PEA_(—)1_T5 (SEQ ID NO: 639), S67314_PEA_(—)1_T6 (SEQ ID NO: 640) and S67314_PEA_(—)1_T7 (SEQ ID NO: 641. Table 19 below describes the starting and ending position of this segment on each transcript.

TABLE 19 Segment location on transcripts Segment Segment Transcript starting ending name position position S67314_PEA_1_T4 998 1170 (SEQ ID NO:638) S67314_PEA_1_T5 998 1170 (SEQ ID NO:639) S67314_PEA_1_T6 998 1170 (SEQ ID NO:640) S67314_PEA_1_T7 1031 1203 (SEQ ID NO:641

According to an optional embodiment of the present invention, short segments related to the above cluster are also provided. These segments are up to about 120 bp in length, and so are included in a separate description.

Segment cluster S67314_PEA_(—)1_node_(—)10 (SEQ ID NO: 648) according to the present invention is supported by 64 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): S67314_PEA_(—)1_T4 (SEQ ID NO: 638), S67314_PEA_(—)1_T5 (SEQ ID NO: 639), S67314_PEA_(—)1_T6 (SEQ ID NO: 640) and S67314_PEA_(—)1_T7 (SEQ ID NO: 641. Table 20 below describes the starting and ending position of this segment on each transcript.

TABLE 20 Segment location on transcripts Segment Segment Transcript starting ending name position position S67314_PEA_1_T4 1171 1272 (SEQ ID NO:638) S67314_PEA_1_T5 1171 1272 (SEQ ID NO:639) S67314_PEA_1_T6 1171 1272 (SEQ ID NO:640) S67314_PEA_1_T7 1204 1305 (SEQ ID NO:641

Segment cluster S67314_PEA_(—)1_node_(—)3 (SEQ ID NO: 649) according to the present invention is supported by 1 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): S67314_PEA_(—)1_T7 (SEQ ID NO: 641. Table 21 below describes the starting and ending position of this segment on each transcript.

TABLE 2 Segment location on transcripts Segment Segment Transcript name starting position ending position S67314_PEA_1_T7 998 1030 (SEQ ID NO: 641 Variant protein alignment to the previously known protein: Sequence name: /tmp/EQ0nMn6tqU/R73CUVKUk5:FABH_HUMAN Sequence documentation:

Alignment of: S67314_PEA_(—)1_P4 (SEQ ID NO: 651) x FABH_HUMAN ..

Alignment segment 1/1:

Quality: 1095.00 Escore: 0 Matching length: 115 Total length: 115 Matching Percent Similarity: 100.00 Matching Percent Identity: 100.00 Total Percent Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0

Alignment:

Sequence name: /tmp/EQ0nMn6tqU/R73CUVKUk5:AAP35373 Sequence documentation:

Alignment of: S67314_PEA_(—)1_P4 (SEQ ID NO: 651) x AAP35373 ..

Alignment segment 1/1:

Quality: 1107.00 Escore: 0 Matching length: 116 Total length: 116 Matching Percent Similarity: 100.00 Matching Percent Identity: 100.00 Total Percent Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0

Alignment:

Sequence name: /tmp/ql4YPIBbdQ/SeofJfCmJW:FABH_HUMAN Sequence documentation:

Alignment of: S67314_PEA_(—)1_P5 (SEQ ID NO: 652) x FABH_HUMAN ..

Alignment segment 1/1:

Quality: 1095.00 Escore: 0 Matching length: 115 Total Length: 115 Matching Percent Similarity: 100.00 Matching Percent Identity: 100.00 Total Percent Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0

Alignment:

Sequence name: /tmp/ql4YPIBbdQ/SeofJfCmJW:AAP35373 Sequence documentation:

Alignment of: S67314_PEA_(—)1_P5 (SEQ ID NO: 652) x AAP35373 ..

Alignment segment 1/1:

Quality: 1107.00 Escore: 0 Matching length: 116 Total length: 116 Matching Percent Similarity: 100.00 Matching Percent Identity: 100.00 Total Percent Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0

Alignment:

Sequence name: /tmp/PXra2DxL1v/Q8GTrzNMVX:FABH_HUMAN Sequence documentation:

Alignment of: S67314_PEA_(—)1_P6 (SEQ ID NO: 653) x FABH_HUMAN ..

Alignment segment 1/1:

Quality: 1095.00 Escore: 0 Matching length: 115 Total length: 115 Matching Percent Similarity: 100.00 Matching Percent Identity: 100.00 Total Percent Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0

Alignment:

Sequence name: /tmp/PXra2DxL1v/Q8GTrzNMVX:AAP35373 Sequence documentation:

Alignment of: S67314_PEA_(—)1_P6 (SEQ ID NO: 653) x AAP35373 ..

Alignment segment 1/1:

Quality: 1107.00 Escore: 0 Matching length: 116 Total length: 116 Matching Percent Similarity: 100.00 Matching Percent Identity: 100.00 Total Percent Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0

Alignment:

Sequence name: /tmp/xYzWyViDom/twDu3T69pd:FABH_HUMAN Sequence documentation:

Alignment of: S67314_PEA_(—)1_P7 (SEQ ID NO: 654) x FABH_HUMAN ..

Alignment segment 1/1:

Quality: 1160.00 Escore: 0 Matching length: 132 Total length: 143 Matching Percent Similarity: 100.00 Matching Percent Identity: 100.00 Total Percent Similarity: 92.31 Total Percent Identity: 92.31 Gaps: 1

Alignment:

Sequence name: /tmp/xYzWyViDom/twDu3T69pd:AAP35373 Sequence documentation:

Alignment of: S67314_PEA_(—)1_P7 (SEQ ID NO: 654) x AAP35373 ..

Alignment segment 1/1:

Quality: 1172.00 Escore: 0 Matching length: 133 Total length: 144 Matching Percent Similarity: 100.00 Matching Percent Identity: 100.00 Total Percent Similarity: 92.36 Total Percent Identity: 92.36 Gaps: 1

Alignment:

Description for Cluster Z39337

Cluster Z39337 features 3 transcript(s) and 12 segment(s) of interest, the names for which are given in Tables 1 and 2, respectively, the sequences themselves are given at the end of the application. The selected protein variants are given in table 3.

TABLE 1 Transcripts of interest Transcript Name SEQ ID NO: Z39337_PEA_2_PEA_1_T3 655 Z39337_PEA_2_PEA_1_T6 656 Z39337_PEA_2_PEA_1_T12 657

TABLE 2 Segments of interest Segment Name SEQ ID NO: Z39337_PEA_2_PEA_1_node_2 658 Z39337_PEA_2_PEA_1_node_15 659 Z39337_PEA_2_PEA_1_node_16 660 Z39337_PEA_2_PEA_1_node_18 661 Z39337_PEA_2_PEA_1_node_21 662 Z39337_PEA_2_PEA_1_node_22 663 Z39337_PEA_2_PEA_1_node_3 664 Z39337_PEA_2_PEA_1_node_5 665 Z39337_PEA_2_PEA_1_node_6 666 Z39337_PEA_2_PEA_1_node_10 667 Z39337_PEA_2_PEA_1_node_11 668 Z39337_PEA_2_PEA_1_node_14 669

TABLE 3 Proteins of interest SEQ ID Corresponding Protein Name NO: Transcript(s) Z39337_PEA_2_PEA_1_P4 671 Z39337_PEA_2_PEA_1_T3 (SEQ ID NO:655) Z39337_PEA_2_PEA_1_P9 672 Z39337_PEA_2_PEA_1_T12 (SEQ ID NO:657) Z39337_PEA_2_PEA_1_P13 673 Z39337_PEA_2_PEA_1_T6 (SEQ ID NO:656)

These sequences are variants of the known protein Kallikrein 6 precursor (SwissProt accession identifier KLK6_HUMAN; known also according to the synonyms EC 3.4.21.-; Protease M; Neurosin; Zyme; SP59), SEQ ID NO: 670, referred to herein as the previously known protein.

The sequence for protein Kallikrein 6 precursor is given at the end of the application, as “Kallikrein 6 precursor amino acid sequence”. Protein Kallikrein 6 precursor localization is believed to be secreted.

The following GO Annotation(s) apply to the previously known protein. The following annotation(s) were found: central nervous system development; response to wounding; protein autoprocessing, which are annotation(s) related to Biological Process; chymotrypsin; tissue kallikrein; trypsin; protein binding; hydrolase, which are annotation(s) related to Molecular Function; and extracellular; cytoplasm, which are annotation(s) related to Cellular Component.

The GO assignment relies on information from one or more of the SwissProt/TremBl Protein knowledgebase, available from <http://www.expasy.ch/sprot/>; or Locuslink, available from <http://www.ncbi.nlm.nih.gov/projects/LocusLink/>.

Cluster Z39337 can be used as a diagnostic marker according to overexpression of transcripts of this cluster in cancer. Expression of such transcripts in normal tissues is also given according to the previously described methods. The term “number” in the left hand column of the table and the numbers on the y-axis of FIG. 34 refer to weighted expression of ESTs in each category, as “parts per million” (ratio of the expression of ESTs for a particular cluster to the expression of all ESTs in that category, according to parts per million).

Overall, the following results were obtained as shown with regard to the histograms in FIG. 34 and Table 4. This cluster is overexpressed (at least at a minimum level) in the following pathological conditions: epithelial malignant tumors and gastric carcinoma.

TABLE 4 Normal tissue distribution Name of Tissue Number brain 56 colon 0 epithelial 3 general 11 head and neck 0 kidney 26 breast 52 ovary 0 prostate 0 stomach 0 uterus 0

TABLE 5 P values and ratios for expression in cancerous tissue Name of Tissue P1 P2 SP1 R3 SP2 R4 brain 8.0e−01 8.4e−01 9.6e−01 0.5 1 0.3 colon 1.2e−01 8.1e−02 4.9e−01 1.9 7.4e−02 2.2 epithelial 2.0e−02 1.8e−02 1.0e−05 4.3 7.8e−15 6.9 general 4.1e−02 1.1e−01 4.3e−06 2.3 1.6e−16 2.6 head and neck 2.1e−01 3.3e−01 1 1.7 1 1.2 kidney 8.9e−01 9.2e−01 8.2e−01 0.8 9.1e−01 0.6 breast 9.1e−01 9.1e−01 1 0.5 9.7e−01 0.6 ovary 1.4e−01 1.7e−01 4.7e−03 2.9 2.4e−02 2.2 prostate 7.3e−01 7.8e−01 4.5e−01 2.0 5.6e−01 1.7 stomach 3.6e−01 1.1e−01 1 1.0 8.9e−08 5.3 uterus 4.7e−01 4.0e−01 1.9e−01 2.0 3.3e−01 1.7

As noted above, cluster Z39337 features 3 transcript(s), which were listed in Table 1 above. These transcript(s) encode for protein(s) which are variant(s) of protein Kallikrein 6 precursor. A description of each variant protein according to the present invention is now provided.

Variant protein Z39337_PEA_(—)2_PEA_(—)1_P4 (SEQ ID NO: 671) according to the present invention has an amino acid sequence as given at the end of the application; it is encoded by transcript(s) Z39337_PEA_(—)2_PEA_(—)1_T3 (SEQ ID NO: 655). An alignment is given to the known protein (Kallikrein 6 precursor) at the end of the application. One or more alignments to one or more previously published protein sequences are given at the end of the application. A brief description of the relationship of the variant protein according to the present invention to each such aligned protein is as follows:

Comparison report between Z39337_PEA_(—)2_PEA_(—)1_P4 (SEQ ID NO: 671) and KLK6_HUMAN:

1. An isolated chimeric polypeptide encoding for Z39337_PEA_(—)2_PEA_(—)1_P4 (SEQ ID NO: 671), comprising a first amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence MWLPLSGAA (SEQ ID NO: 1083) corresponding to amino acids 1-9 of Z39337_PEA_(—)2_PEA_(—)1_P4 (SEQ ID NO: 671), and a second amino acid sequence being at least 90% homologous to MKKLMVVLSLIAAAWAEEQNKLVHGGPCDKTSHPYQAALYTSGHLLCGGVLIHPLWV LTAAHCKKPNLQVFLGKHNLRQRESSQEQSSVVRAVIHPDYDAASHDQDIMLLRLARP AKLSELIQPLPLERDCSANTTSCHILGWGKTADGDFPDTIQCAYIHLVSREECEHAYPGQ ITQNMLCAGDEKYGKDSCQGDSGGPLVCGDHLRGLVSWGNIPCGSKEKPGVYTNVCR YTNWIQKTIQAK corresponding to amino acids 1-244 of KLK6_HUMAN, which also corresponds to amino acids 10-253 of Z39337_PEA_(—)2_PEA_(—)1_P4 (SEQ ID NO: 671), wherein said first amino acid sequence and second amino acid sequence are contiguous and in a sequential order.

2. An isolated polypeptide encoding for a head of Z39337_PEA_(—)2_PEA_(—)1_P4 (SEQ ID NO: 671), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence MWLPLSGAA (SEQ ID NO: 1083) of Z39337_PEA_(—)2_PEA_(—)1_P4 (SEQ ID NO: 671).

The location of the variant protein was determined according to results from a number of different software programs and analyses, including analyses from SignalP and other specialized programs. The variant protein is believed to be located as follows with regard to the cell: secreted. The protein localization is believed to be secreted because both signal-peptide prediction programs predict that this protein has a signal peptide, and neither trans-membrane region prediction program predicts that this protein has a trans-membrane region.

Variant protein Z39337_PEA_(—)2_PEA_(—)1_P4 (SEQ ID NO: 671) also has the following non-silent SNPs (Single Nucleotide Polymorphisms) as listed in Table 6, (given according to their position(s) on the amino acid sequence, with the alternative amino acid(s) listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein Z39337_PEA_(—)2_PEA_(—)1_P4 (SEQ ID NO: 671) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 6 Amino acid mutations SNP position(s) on amino acid sequence Alternative amino acid(s) Previously known SNP? 238 N -> No

The glycosylation sites of variant protein Z39337_PEA_(—)2_PEA_(—)1_P4 (SEQ ID NO: 671), as compared to the known protein Kallikrein 6 precursor, are described in Table 7 (given according to their position(s) on the amino acid sequence in the first column; the second column indicates whether the glycosylation site is present in the variant protein; and the last column indicates whether the position is different on the variant protein).

TABLE 7 Glycosylation site(s) Position(s) on known Present amino acid sequence in variant protein? Position in variant protein? 134 yes 143

Variant protein Z39337_PEA_(—)2_PEA_(—)1_P4 (SEQ ID NO: 671) is encoded by the following transcript(s): Z39337_PEA_(—)2_PEA_(—)1_T3 (SEQ ID NO: 655), for which the sequence(s) is/are given at the end of the application. The coding portion of transcript Z39337_PEA_(—)2_PEA_(—)1_T3 (SEQ ID NO: 655) is shown in bold; this coding portion starts at position 87 and ends at position 845. The transcript also has the following SNPs as listed in Table 8 (given according to their position on the nucleotide sequence, with the alternative nucleic acid listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein Z39337_PEA_(—)2_PEA_(—)1_P4 (SEQ ID NO: 671) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 8 Nucleic acid SNPs SNP position on nucleotide sequence Alternative nucleic acid Previously known SNP? 87 A -> G Yes 396 -> G No 599 G -> C Yes 799 A -> No 995 C -> No 995 C -> G No 1184 C -> No 1294 T -> A Yes

Variant protein Z39337_PEA_(—)2_PEA_(—)1_P9 (SEQ ID NO: 672) according to the present invention has an amino acid sequence as given at the end of the application; it is encoded by transcript(s) Z39337_PEA_(—)2_PEA_(—)1_T12 (SEQ ID NO: 657). An alignment is given to the known protein (Kallikrein 6 precursor) at the end of the application. One or more alignments to one or more previously published protein sequences are given at the end of the application. A brief description of the relationship of the variant protein according to the present invention to each such aligned protein is as follows:

Comparison report between Z39337_PEA_(—)2_PEA_(—)1_P9 (SEQ ID NO: 672) and KLK6_HUMAN:

1. An isolated chimeric polypeptide encoding for Z39337_PEA_(—)2_PEA_(—)1_P9 (SEQ ID NO: 672), comprising a first amino acid sequence being at least 90% homologous to MKKLMVVLSLIAAAWAEEQNKLVHGGPCDKTSHPYQAALYTSGHLLCGGVLIHPLWV LTAAHCKKPNLQVFLGKHNLRQRESSQEQSSVVRAVIHPDYDAASHDQDIMLLRLARP AKLSELIQPLPLERDCSANTTSCHILGWGKTADG corresponding to amino acids 1-149 of KLK6_HUMAN, which also corresponds to amino acids 1-149 of Z39337_PEA_(—)2_PEA_(—)1_P9 (SEQ ID NO: 672), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence Q corresponding to amino acids 150-150 of Z39337_PEA_(—)2_PEA_(—)1_P9 (SEQ ID NO: 672), wherein said first amino acid sequence and second amino acid sequence are contiguous and in a sequential order.

The location of the variant protein was determined according to results from a number of different software programs and analyses, including analyses from SignalP and other specialized programs. The variant protein is believed to be located as follows with regard to the cell: secreted. The protein localization is believed to be secreted because both signal-peptide prediction programs predict that this protein has a signal peptide.

The glycosylation sites of variant protein Z39337_PEA_(—)2_PEA_(—)1_P9 (SEQ ID NO: 672), as compared to the known protein Kallikrein 6 precursor, are described in Table 9 (given according to their position(s) on the amino acid sequence in the first column; the second column indicates whether the glycosylation site is present in the variant protein; and the last column indicates whether the position is different on the variant protein).

TABLE 9 Glycosylation site(s) Position(s) on known Present amino acid sequence in variant protein? Position in variant protein? 134 yes 134

Variant protein Z39337_PEA_(—)2_PEA_(—)1_P9 (SEQ ID NO: 672) is encoded by the following transcript(s): Z39337_PEA_(—)2_PEA_(—)1_T12 (SEQ ID NO: 657), for which the sequence(s) is/are given at the end of the application. The coding portion of transcript Z39337_PEA_(—)2_PEA_(—)1_T12 (SEQ ID NO: 657) is shown in bold; this coding portion starts at position 298 and ends at position 747. The transcript also has the following SNPs as listed in Table 10 (given according to their position on the nucleotide sequence, with the alternative nucleic acid listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein Z39337_PEA_(—)2_PEA_(—)1_P9 (SEQ ID NO: 672) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 10 Nucleic acid SNPs SNP position on nucleotide sequence Alternative nucleic acid Previously known SNP? 81 G -> No 102 G -> T Yes 147 G -> A Yes 270 G -> No 270 G -> A No 580 -> G No 784 T -> C Yes 802 G -> A Yes

Variant protein Z39337_PEA_(—)2_PEA_(—)1_P13 (SEQ ID NO: 673) according to the present invention has an amino acid sequence as given at the end of the application; it is encoded by transcript(s) Z39337_PEA_(—)2_PEA_(—)1_T6 (SEQ ID NO: 656). The location of the variant protein was determined according to results from a number of different software programs and analyses, including analyses from SignalP and other specialized programs. The variant protein is believed to be located as follows with regard to the cell: secreted. The protein localization is believed to be secreted because both signal-peptide prediction programs predict that this protein has a signal peptide, and neither trans-membrane region prediction program predicts that this protein has a trans-membrane region.

Variant protein Z39337_PEA_(—)2_PEA_(—)1_P13 (SEQ ID NO: 673) is encoded by the following transcript(s): Z39337_PEA_(—)2_PEA_(—)1_T6 (SEQ ID NO: 656), for which the sequence(s) is/are given at the end of the application. The coding portion of transcript Z39337_PEA_(—)2_PEA_(—)1_T6 (SEQ ID NO: 656) is shown in bold; this coding portion starts at position 298 and ends at position 417. The transcript also has the following SNPs as listed in Table 11 (given according to their position on the nucleotide sequence, with the alternative nucleic acid listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein Z39337_PEA_(—)2_PEA_(—)1_P13 (SEQ ID NO: 673) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 11 Nucleic acid SNPs SNP position on nucleotide Alternative sequence nucleic acid Previously known SNP? 81 G -> No 102 G -> T Yes 147 G -> A Yes 270 G -> No 270 G -> A No 423 -> G No 626 G -> C Yes 826 A -> No 1022 C -> No 1022 C -> G No 1211 C -> No 1321 T -> A Yes

As noted above, cluster Z39337 features 12 segment(s), which were listed in Table 2 above and for which the sequence(s) are given at the end of the application. These segment(s) are portions of nucleic acid sequence(s) which are described herein separately because they are of particular interest. A description of each segment according to the present invention is now provided.

Segment cluster Z39337_PEA_(—)2_PEA_(—)1_node_(—)2 (SEQ ID NO: 658) according to the present invention is supported by 23 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): Z39337_PEA_(—)2_PEA_(—)1_T6 (SEQ ID NO: 656) and Z39337_PEA_(—)2_PEA_(—)1_T12 (SEQ ID NO: 657). Table 12 below describes the starting and ending position of this segment on each transcript.

TABLE 12 Segment location on transcripts Segment Segment starting ending Transcript name position position Z39337_PEA_2_PEA_1_T6 1 237 (SEQ ID NO: 656) Z39337_PEA_2_PEA_1_T12 1 237 (SEQ ID NO: 657)

Segment cluster Z39337_PEA_(—)2_PEA_(—)1_node_(—)15 (SEQ ID NO: 659) according to the present invention is supported by 54 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): Z39337_PEA_(—)2_PEA_(—)1_T3 (SEQ ID NO: 655), Z39337_PEA_(—)2_PEA_(—)1_T6 (SEQ ID NO: 656) and Z39337_PEA_(—)2_PEA_(—)1_T12 (SEQ ID NO: 657). Table 13 below describes the starting and ending position of this segment on each transcript.

TABLE 13 Segment location on transcripts Segment Segment starting ending Transcript name position position Z39337_PEA_2_PEA_1_T3 363 558 (SEQ ID NO: 655) Z39337_PEA_2_PEA_1_T6 390 585 (SEQ ID NO: 656) Z39337_PEA_2_PEA_1_T12 547 742 (SEQ ID NO: 657)

Segment cluster Z39337_PEA_(—)2_PEA_(—)1_node_(—)16 (SEQ ID NO: 660) according to the present invention is supported by 2 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): Z39337_PEA_(—)2_PEA_(—)1_T12 (SEQ ID NO: 657). Table 14 below describes the starting and ending position of this segment on each transcript.

TABLE 14 Segment location on transcripts Segment Segment starting ending Transcript name position position Z39337_PEA_2_PEA_1_T12 743 1402 (SEQ ID NO: 657)

Segment cluster Z39337_PEA_(—)2_PEA_(—)1_node_(—)18 (SEQ ID NO: 661) according to the present invention is supported by 53 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): Z39337_PEA_(—)2_PEA_(—)1_T3 (SEQ ID NO: 655) and Z39337_PEA_(—)2_PEA_(—)1_T6 (SEQ ID NO: 656). Table 15 below describes the starting and ending position of this segment on each transcript.

TABLE 15 Segment location on transcripts Segment Segment starting ending Transcript name position position Z39337_PEA_2_PEA_1_T3 559 695 (SEQ ID NO: 655) Z39337_PEA_2_PEA_1_T6 586 722 (SEQ ID NO: 656)

Segment cluster Z39337_PEA_(—)2_PEA_(—)1_node_(—)21 (SEQ ID NO: 662) according to the present invention is supported by 81 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): Z39337_PEA_(—)2_PEA_(—)1_T3 (SEQ ID NO: 655) and Z39337_PEA_(—)2_PEA_(—)1_T6 (SEQ ID NO: 656). Table 16 below describes the starting and ending position of this segment on each transcript.

TABLE 16 Segment location on transcripts Segment Segment starting ending Transcript name position position Z39337_PEA_2_PEA_1_T3 696 1112 (SEQ ID NO: 655) Z39337_PEA_2_PEA_1_T6 723 1139 (SEQ ID NO: 656)

Microarray (chip) data is also available for this segment as follows. As described above with regard to the cluster itself, various oligonucleotides were tested for being differentially expressed in various disease conditions, particularly cancer. The following oligonucleotides were found to hit this segment (with regard to ovarian cancer), shown in Table 17.

TABLE 17 Oligonucleotides related to this segment Oligonucleotide name Overexpressed in cancers Chip reference Z39337_0_9_0 (SEQ ID ovarian carcinoma OVA NO: 1024)

Segment cluster Z39337_PEA_(—)2_PEA_(—)1_node_(—)22 (SEQ ID NO: 663) according to the present invention is supported by 58 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): Z39337_PEA_(—)2_PEA_(—)1_T3 (SEQ ID NO: 655) and Z39337_PEA_(—)2_PEA_(—)1_T6 (SEQ ID NO: 656). Table 18 below describes the starting and ending position of this segment on each transcript.

TABLE 18 Segment location on transcripts Segment Segment starting ending Transcript name position position Z39337_PEA_2_PEA_1_T3 1113 1387 (SEQ ID NO: 655) Z39337_PEA_2_PEA_1_T6 1140 1414 (SEQ ID NO: 656)

According to an optional embodiment of the present invention, short segments related to the above cluster are also provided. These segments are up to about 120 bp in length, and so are included in a separate description.

Segment cluster Z39337_PEA_(—)2_PEA_(—)1_node_(—)3 (SEQ ID NO: 664) according to the present invention is supported by 55 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): Z39337_PEA_(—)2_PEA_(—)1_T6 (SEQ ID NO: 656) and Z39337_PEA_(—)2_PEA_(—)1_T12 (SEQ ID NO: 657). Table 19 below describes the starting and ending position of this segment on each transcript.

TABLE 19 Segment location on transcripts Segment Segment starting ending Transcript name position position Z39337_PEA_2_PEA_1_T6 238 289 (SEQ ID NO: 656) Z39337_PEA_2_PEA_1_T12 238 289 (SEQ ID NO: 657)

Segment cluster Z39337_PEA_(—)2_PEA_(—)1_node_(—)5 (SEQ ID NO: 665) according to the present invention is supported by 1 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): Z39337_PEA_(—)2_PEA_(—)1_T3 (SEQ ID NO: 655). Table 20 below describes the starting and ending position of this segment on each transcript.

TABLE 20 Segment location on transcripts Segment Segment starting ending Transcript name position position Z39337_PEA_2_PEA_1_T3 1 105 (SEQ ID NO: 655)

Segment cluster Z39337_PEA_(—)2_PEA_(—)1_node_(—)6 (SEQ ID NO: 666) according to the present invention is supported by 56 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): Z39337_PEA_(—)2_PEA_(—)1_T3 (SEQ ID NO: 655), Z39337_PEA_(—)2_PEA_(—)1_T6 (SEQ ID NO: 656) and Z39337_PEA_(—)2_PEA_(—)1_T12 (SEQ ID NO: 657). Table 21 below describes the starting and ending position of this segment on each transcript.

TABLE 21 Segment location on transcripts Segment Segment starting ending Transcript name position position Z39337_PEA_2_PEA_1_T3 106 153 (SEQ ID NO: 655) Z39337_PEA_2_PEA_1_T6 290 337 (SEQ ID NO: 656) Z39337_PEA_2_PEA_1_T12 290 337 (SEQ ID NO: 657)

Segment cluster Z39337_PEA_(—)2_PEA_(—)1_node_(—)10 (SEQ ID NO: 667) according to the present invention is supported by 60 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): Z39337_PEA_(—)2_PEA_(—)1_T3 (SEQ ID NO: 655) and Z39337_PEA_(—)2_PEA_(—)1_T12 (SEQ ID NO: 657). Table 22 below describes the starting and ending position of this segment on each transcript.

TABLE 22 Segment location on transcripts Segment Segment starting ending Transcript name position position Z39337_PEA_2_PEA_1_T3 154 207 (SEQ ID NO: 655) Z39337_PEA_2_PEA_1_T12 338 391 (SEQ ID NO: 657)

Segment cluster Z39337_PEA_(—)2_PEA_(—)1_node_(—)11 (SEQ ID NO: 668) according to the present invention is supported by 58 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): Z39337_PEA_(—)2_PEA_(—)1_T3 (SEQ ID NO: 655) and Z39337_PEA_(—)2_PEA_(—)1_T12 (SEQ ID NO: 657). Table 23 below describes the starting and ending position of this segment on each transcript.

TABLE 23 Segment location on transcripts Segment Segment starting ending Transcript name position position Z39337_PEA_2_PEA_1_T3 208 310 (SEQ ID NO: 655) Z39337_PEA_2_PEA_1_T12 392 494 (SEQ ID NO: 657)

Segment cluster Z39337_PEA_(—)2_PEA_(—)1_node_(—)14 (SEQ ID NO: 669) according to the present invention is supported by 49 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): Z39337_PEA_(—)2_PEA_(—)1_T3 (SEQ ID NO: 655), Z39337_PEA_(—)2_PEA_(—)1_T6 (SEQ ID NO: 656) and Z39337_PEA_(—)2_PEA_(—)1_T12 (SEQ ID NO: 657). Table 24 below describes the starting and ending position of this segment on each transcript.

TABLE 24 Segment location on transcripts Segment Segment starting ending Transcript name position position Z39337_PEA_2_PEA_1_T3 311 362 (SEQ ID NO: 655) Z39337_PEA_2_PEA_1_T6 338 389 (SEQ ID NO: 656) Z39337_PEA_2_PEA_1_T12 495 546 (SEQ ID NO: 657) Variant protein alignment to the previously known protein: Sequence name: KLK6_HUMAN Sequence documentation:

Alignment of: Z39337_PEA_(—)2_PEA_(—)1_P4 (SEQ ID NO: 671) x KLK6_HUMAN ..

Alignment segment 1/1:

Quality: 2444.00 Escore: 0 Matching length: 244 Total length: 244 Matching Percent Similarity: 100.00 Matching Percent Identity: 100.00 Total Percent Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0

Alignment:

Sequence name: KLK6_HUMAN Sequence documentation:

Alignment of: Z39337_PEA_(—)2_PEA_(—)1_P9 (SEQ ID NO: 672) x KLK6_HUMAN ..

Alignment segment 1/1:

Quality: 1471.00 Escore: 0 Matching length: 149 Total length: 149 Matching Percent Similarity: 100.00 Matching Percent Identity: 100.00 Total Percent Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0

Alignment:

Description for Cluster HUMPHOSLIP

Cluster HUMPHOSLIP features 7 transcript(s) and 53 segment(s) of interest, the names for which are given in Tables 1 and 2, respectively, the sequences themselves are given at the end of the application. The selected protein variants are given in table 3.

TABLE 1 Transcripts of interest Transcript Name SEQ ID NO: HUMPHOSLIP_PEA_2_T6 674 HUMPHOSLIP_PEA_2_T7 675 HUMPHOSLIP_PEA_2_T14 676 HUMPHOSLIP_PEA_2_T16 677 HUMPHOSLIP_PEA_2_T17 678 HUMPHOSLIP_PEA_2_T18 679 HUMPHOSLIP_PEA_2_T19 680

TABLE 2 Segments of interest Segment Name SEQ ID NO: HUMPHOSLIP_PEA_2_node_0 681 HUMPHOSLIP_PEA_2_node_19 682 HUMPHOSLIP_PEA_2_node_34 683 HUMPHOSLIP_PEA_2_node_68 684 HUMPHOSLIP_PEA_2_node_70 685 HUMPHOSLIP_PEA_2_node_75 686 HUMPHOSLIP_PEA_2_node_2 687 HUMPHOSLIP_PEA_2_node_3 688 HUMPHOSLIP_PEA_2_node_4 689 HUMPHOSLIP_PEA_2_node_6 690 HUMPHOSLIP_PEA_2_node_7 691 HUMPHOSLIP_PEA_2_node_8 692 HUMPHOSLIP_PEA_2_node_9 693 HUMPHOSLIP_PEA_2_node_14 694 HUMPHOSLIP_PEA_2_node_15 695 HUMPHOSLIP_PEA_2_node_16 696 HUMPHOSLIP_PEA_2_node_17 697 HUMPHOSLIP_PEA_2_node_23 698 HUMPHOSLIP_PEA_2_node_24 699 HUMPHOSLIP_PEA_2_node_25 700 HUMPHOSLIP_PEA_2_node_26 701 HUMPHOSLIP_PEA_2_node_29 702 HUMPHOSLIP_PEA_2_node_30 703 HUMPHOSLIP_PEA_2_node_33 704 HUMPHOSLIP_PEA_2_node_36 705 HUMPHOSLIP_PEA_2_node_37 706 HUMPHOSLIP_PEA_2_node_39 707 HUMPHOSLIP_PEA_2_node_40 708 HUMPHOSLIP_PEA_2_node_41 709 HUMPHOSLIP_PEA_2_node_42 710 HUMPHOSLIP_PEA_2_node_44 711 HUMPHOSLIP_PEA_2_node_45 712 HUMPHOSLIP_PEA_2_node_47 713 HUMPHOSLIP_PEA_2_node_51 714 HUMPHOSLIP_PEA_2_node_52 715 HUMPHOSLIP_PEA_2_node_53 716 HUMPHOSLIP_PEA_2_node_54 717 HUMPHOSLIP_PEA_2_node_55 718 HUMPHOSLIP_PEA_2_node_58 719 HUMPHOSLIP_PEA_2_node_59 720 HUMPHOSLIP_PEA_2_node_60 721 HUMPHOSLIP_PEA_2_node_61 722 HUMPHOSLIP_PEA_2_node_62 723 HUMPHOSLIP_PEA_2_node_63 724 HUMPHOSLIP_PEA_2_node_64 725 HUMPHOSLIP_PEA_2_node_65 726 HUMPHOSLIP_PEA_2_node_66 727 HUMPHOSLIP_PEA_2_node_67 728 HUMPHOSLIP_PEA_2_node_69 729 HUMPHOSLIP_PEA_2_node_71 730 HUMPHOSLIP_PEA_2_node_72 731 HUMPHOSLIP_PEA_2_node_73 732 HUMPHOSLIP_PEA_2_node_74 733

TABLE 3 Proteins of interest SEQ ID Corresponding Protein Name NO: Transcript(s) HUMPHOSLIP_PEA_2_P10 735 HUMPHOSLIP_PEA_2_T17 (SEQ ID NO:678) HUMPHOSLIP_PEA_2_P12 736 HUMPHOSLIP_PEA_2_T19 (SEQ ID NO:680) HUMPHOSLIP_PEA_2_P30 737 HUMPHOSLIP_PEA_2_T6 (SEQ ID NO:674) HUMPHOSLIP_PEA_2_P31 738 HUMPHOSLIP_PEA_2_T7 (SEQ ID NO:675) HUMPHOSLIP_PEA_2_P33 739 HUMPHOSLIP_PEA_2_T14 (SEQ ID NO:676) HUMPHOSLIP_PEA_2_P34 740 HUMPHOSLIP_PEA_2_T16 (SEQ ID NO:677) HUMPHOSLIP_PEA_2_P35 741 HUMPHOSLIP_PEA_2_T18 (SEQ ID NO:679)

These sequences are variants of the known protein Phospholipid transfer protein precursor (SwissProt accession identifier PLTP_HUMAN; known also according to the synonyms Lipid transfer protein II), SEQ ID NO: 734, referred to herein as the previously known protein.

Protein Phospholipid transfer protein precursor is known or believed to have the following function(s): Converts HDL into larger and smaller particles. May play a key role in extracellular phospholipid transport and modulation of HDL particles. The sequence for protein Phospholipid transfer protein precursor is given at the end of the application, as “Phospholipid transfer protein precursor amino acid sequence”. Known polymorphisms for this sequence are as shown in Table 4.

TABLE 4 Amino acid mutations for Known Protein SNP position(s) on amino acid sequence Comment 282 R -> Q. /FTId = VAR_017020. 372 R -> H. /FTId = VAR_017021. 380 R -> W (in dbSNP: 6065903). /FTId = VAR_017022. 444 F -> L (in dbSNP: 1804161). /FTId = VAR_012073. 487 T -> K (in dbSNP: 1056929). /FTId = VAR_012074. 18 E -> V

Protein Phospholipid transfer protein precursor localization is believed to be Secreted.

The following GO Annotation(s) apply to the previously known protein. The following annotation(s) were found: lipid metabolism; lipid transport, which are annotation(s) related to Biological Process; lipid binding, which are annotation(s) related to Molecular Function; and extracellular, which are annotation(s) related to Cellular Component.

The GO assignment relies on information from one or more of the SwissProt/TremBl Protein knowledgebase, available from <http://www.expasy.ch/sprot/>; or Locuslink, available from <http://www.ncbi.nlm.nih.gov/projects/LocusLink/>.

For this cluster, at least one oligonucleotide was found to demonstrate overexpression of the cluster, although not of at least one transcript/segment as listed below. Microarray (chip) data is also available for this cluster as follows. Various oligonucleotides were tested for being differentially expressed in various disease conditions, particularly cancer, as previously described. The following oligonucleotides were found to hit this cluster but not other segments/transcripts below (with regard to ovarian cancer), shown in Table 5.

TABLE 5 Oligonucleotides related to this cluster Overexpressed in Chip Oligonucleotide name cancers reference HUMPHOSLIP_0_0_18458 ovarian carcinoma OVA (SEQ ID NO: 1025)

As noted above, cluster HUMPHOSLIP features 7 transcript(s), which were listed in Table 1 above. These transcript(s) encode for protein(s) which are variant(s) of protein Phospholipid transfer protein precursor. A description of each variant protein according to the present invention is now provided.

Variant protein HUMPHOSLIP_PEA_(—)2_P10 (SEQ ID NO: 735) according to the present invention has an amino acid sequence as given at the end of the application; it is encoded by transcript(s) HUMPHOSLIP_PEA_(—)2_T17 (SEQ ID NO: 678). An alignment is given to the known protein (Phospholipid transfer protein precursor) at the end of the application. One or more alignments to one or more previously published protein sequences are given at the end of the application. A brief description of the relationship of the variant protein according to the present invention to each such aligned protein is as follows:

Comparison report between HUMPHOSLIP_PEA_(—)2_P10 (SEQ ID NO: 735) and PLTP_HUMAN:

1. An isolated chimeric polypeptide encoding for HUMPHOSLIP_PEA_(—)2_P10 (SEQ ID NO: 735), comprising a first amino acid sequence being at least 90% homologous to MALFGALFLALLAGAHAEFPGCKIRVTSKALELVKQEGLRFLEQELETITIPDLRGKEGH FYYNISE corresponding to amino acids 1-67 of PLTP_HUMAN, which also corresponds to amino acids 1-67 of HUMPHOSLIP_PEA_(—)2_P10 (SEQ ID NO: 735), and a second amino acid sequence being at least 90% homologous to KVYDFLSTFITSGMRFLLNQQICPVLYHAGTVLLNSLLDTVPVRSSVDELVGIDYSLMK DPVASTSNLDMDFRGAFFPLTERNWSLPNRAVEPQLQEEERMVYVAFSEFFFDSAMES YFRAGALQLLLVGDKVPHDLDMLLRATYFGSIVLLSPAVIDSPLKLELRVLAPPRCTIKP SGTTISVTASVTIALVPPDQPEVQLSSMTMDARLSAKMALRGKALRTQLDLRRFRIYSN HSALESLALIPLQAPLKTMLQIGVMPMLNERTWRGVQIPLPEGINFVHEVVTNHAGFLTI GADLHFAKGLREVIEKNRPADVRASTAPTPSTAAV corresponding to amino acids 163-493 of PLTP_HUMAN, which also corresponds to amino acids 68-398 of HUMPHOSLIP_PEA_(—)2_P10 (SEQ ID NO: 735), wherein said first amino acid sequence and second amino acid sequence are contiguous and in a sequential order.

2. An isolated chimeric polypeptide encoding for an edge portion of HUMPHOSLIP_PEA_(—)2_P10 (SEQ ID NO: 735), comprising a polypeptide having a length “n”, wherein n is at least about 10 amino acids in length, optionally at least about 20 amino acids in length, preferably at least about 30 amino acids in length, more preferably at least about 40 amino acids in length and most preferably at least about 50 amino acids in length, wherein at least two amino acids comprise EK, having a structure as follows: a sequence starting from any of amino acid numbers 67-x to 67; and ending at any of amino acid numbers 68+((n−2)−x), in which x varies from 0 to n−2.

The location of the variant protein was determined according to results from a number of different software programs and analyses, including analyses from SignalP and other specialized programs. The variant protein is believed to be located as follows with regard to the cell: secreted. The protein localization is believed to be secreted because both signal-peptide prediction programs predict that this protein has a signal peptide, and neither trans-membrane region prediction program predicts that this protein has a trans-membrane region.

Variant protein HUMPHOSLIP_PEA_(—)2_P10 (SEQ ID NO: 735) also has the following non-silent SNPs (Single Nucleotide Polymorphisms) as listed in Table 6, (given according to their position(s) on the amino acid sequence, with the alternative amino acid(s) listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein HUMPHOSLIP_PEA_(—)2_P10 (SEQ ID NO: 735) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 6 Amino acid mutations SNP position(s) on amino acid sequence Alternative amino acid(s) Previously known SNP? 16 H -> R Yes 18 E -> V Yes 113 S -> F Yes 118 V -> No 140 R -> No 140 R -> P No 150 N -> No 160 P -> No 201 P -> No 274 M -> No 285 R -> W Yes 292 Q -> No 315 L -> * No 330 M -> I Yes 349 F -> L Yes 392 T -> K Yes

The glycosylation sites of variant protein HUMPHOSLIP_PEA_(—)2_P10 (SEQ ID NO: 735), as compared to the known protein Phospholipid transfer protein precursor, are described in Table 7 (given according to their position(s) on the amino acid sequence in the first column; the second column indicates whether the glycosylation site is present in the variant protein; and the last column indicates whether the position is different on the variant protein).

TABLE 7 Glycosylation site(s) Position(s) on known Present in Position in amino acid sequence variant protein? variant protein? 94 no 143 no 64 yes 64 245 yes 150 398 yes 303 117 no

Variant protein HUMPHOSLIP_PEA_(—)2_P10 (SEQ ID NO: 735) is encoded by the following transcript(s): HUMPHOSLIP_PEA_(—)2_T17 (SEQ ID NO: 678), for which the sequence(s) is/are given at the end of the application. The coding portion of transcript HUMPHOSLIP_PEA_(—)2_T17 (SEQ ID NO: 678) is shown in bold; this coding portion starts at position 276 and ends at position 1469. The transcript also has the following SNPs as listed in Table 8 (given according to their position on the nucleotide sequence, with the alternative nucleic acid listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein HUMPHOSLIP_PEA_(—)2_P10 (SEQ ID NO: 735) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 8 Nucleic acid SNPs SNP position on nucleotide sequence Alternative nucleic acid Previously known SNP? 174 G -> T No 175 A -> T No 322 A -> G Yes 328 A -> T Yes 431 G -> A Yes 551 C -> T Yes 613 C -> T Yes 628 T -> No 694 G -> No 694 G -> C No 723 A -> No 753 C -> No 876 C -> No 1037 C -> T Yes 1097 G -> No 1128 C -> T Yes 1149 C -> No 1219 T -> A No 1230 C -> T Yes 1265 G -> C Yes 1322 T -> A Yes 1450 C -> A Yes 1469 C -> T No 1549 C -> T Yes 1565 A -> G No 1565 A -> T No 1630 A -> G Yes 1654 T -> A No 1731 G -> T Yes 1864 G -> A Yes 1893 G -> T Yes 2073 G -> A Yes 2269 C -> T Yes 2325 G -> T Yes 2465 C -> T Yes 2566 C -> T Yes 2881 A -> G No

Variant protein HUMPHOSLIP_PEA_(—)2_P12 (SEQ ID NO: 736) according to the present invention has an amino acid sequence as given at the end of the application; it is encoded by transcript(s) HUMPHOSLIP_PEA_(—)2_T19 (SEQ ID NO: 680). An alignment is given to the known protein (Phospholipid transfer protein precursor) at the end of the application. One or more alignments to one or more previously published protein sequences are given at the end of the application. A brief description of the relationship of the variant protein according to the present invention to each such aligned protein is as follows:

Comparison report between HUMPHOSLIP_PEA_(—)2_P12 (SEQ ID NO: 736) and PLTP_HUMAN:

1. An isolated chimeric polypeptide encoding for HUMPHOSLIP_PEA_(—)2_P12 (SEQ ID NO: 736), comprising a first amino acid sequence being at least 90% homologous to MALFGALFLALLAGAHAEFPGCKIRVTSKALELVKQEGLRFLEQELETITIPDLRGKEGH FYYNISEVKVTELQLTSSELDFQPQQELMLQITNASLGLRFRRQLLYWFFYDGGYINAS AEGVSIRTGLELSRDPAGRMKVSNVSCQASVSRMHAAFGGTFKKVYDFLSTFITSGMRF LLNQQICPVLYHAGTVLLNSLLDTVPVRSSVDELVGIDYSLMKDPVASTSNLDMDFRG AFFPLTERNWSLPNRAVEPQLQEEERMVYVAFSEFFFDSAMESYFRAGALQLLLVGDK VPHDLDMLLRATYFGSIVLLSPAVIDSPLKLELRVLAPPRCTIKPSGTTISVTASVTIALVP PDQPEVQLSSMTMDARLSAKMALRGKALRTQLDLRRFRIYSNHSALESLALIPLQAPLK TMLQIGVMPMLN corresponding to amino acids 1-427 of PLTP_HUMAN, which also corresponds to amino acids 1-427 of HUMPHOSLIP_PEA_(—)2_P12 (SEQ ID NO: 736), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence GKAGV (SEQ ID NO: 1084) corresponding to amino acids 428-432 of HUMPHOSLIP_PEA_(—)2_P12 (SEQ ID NO: 736), wherein said first amino acid sequence and second amino acid sequence are contiguous and in a sequential order.

2. An isolated polypeptide encoding for a tail of HUMPHOSLIP_PEA_(—)2_P12 (SEQ ID NO: 736), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence GKAGV (SEQ ID NO: 1084) in HUMPHOSLIP_PEA_(—)2_P12 (SEQ ID NO: 736).

The location of the variant protein was determined according to results from a number of different software programs and analyses, including analyses from SignalP and other specialized programs. The variant protein is believed to be located as follows with regard to the cell: secreted. The protein localization is believed to be secreted because both signal-peptide prediction programs predict that this protein has a signal peptide, and neither trans-membrane region prediction program predicts that this protein has a trans-membrane region.

Variant protein HUMPHOSLIP_PEA_(—)2_P12 (SEQ ID NO: 736) also has the following non-silent SNPs (Single Nucleotide Polymorphisms) as listed in Table 9, (given according to their position(s) on the amino acid sequence, with the alternative amino acid(s) listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein HUMPHOSLIP_PEA_(—)2_P12 (SEQ ID NO: 736) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 9 Amino acid mutations SNP position(s) on amino acid sequence Alternative amino acid(s) Previously known SNP? 16 H -> R Yes 18 E -> V Yes 81 D -> H Yes 124 S -> Y Yes 160 T -> No 160 T -> N No 208 S -> F Yes 213 V -> No 235 R -> P No 235 R -> No 245 N -> No 255 P -> No 296 P -> No 369 M -> No 380 R -> W Yes 387 Q -> No 410 L -> * No 425 M -> I Yes

The glycosylation sites of variant protein HUMPHOSLIP_PEA_(—)2_P12 (SEQ ID NO: 736), as compared to the known protein Phospholipid transfer protein precursor, are described in Table 10 (given according to their position(s) on the amino acid sequence in the first column; the second column indicates whether the glycosylation site is present in the variant protein; and the last column indicates whether the position is different on the variant protein).

TABLE 10 Glycosylation site(s) Position(s) on known Present in Position in amino acid sequence variant protein? variant protein? 94 yes 94 143 yes 143 64 yes 64 245 yes 245 398 yes 398 117 yes 117

Variant protein HUMPHOSLIP_PEA_(—)2_P12 (SEQ ID NO: 736) is encoded by the following transcript(s): HUMPHOSLIP_PEA_(—)2_T19 (SEQ ID NO: 680), for which the sequence(s) is/are given at the end of the application. The coding portion of transcript HUMPHOSLIP_PEA_(—)2_T19 (SEQ ID NO: 680) is shown in bold; this coding portion starts at position 276 and ends at position 1571. The transcript also has the following SNPs as listed in Table 11 (given according to their position on the nucleotide sequence, with the alternative nucleic acid listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein HUMPHOSLIP_PEA_(—)2_P12 (SEQ ID NO: 736) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 11 Nucleic acid SNPs SNP position on nucleotide sequence Alternative nucleic acid Previously known SNP? 174 G -> T No 175 A -> T No 322 A -> G Yes 328 A -> T Yes 431 G -> A Yes 516 G -> C Yes 644 G -> A Yes 646 C -> A Yes 754 C -> No 754 C -> A No 836 C -> T Yes 898 C -> T Yes 913 T -> No 979 G -> No 979 G -> C No 1008 A -> No 1038 C -> No 1161 C -> No 1322 C -> T Yes 1382 G -> No 1413 C -> T Yes 1434 C -> No 1504 T -> A No 1515 C -> T Yes 1550 G -> C Yes 1690 T -> A Yes 1818 C -> A Yes 1837 C -> T No 1917 C -> T Yes 1933 A -> G No 1933 A -> T No 1998 A -> G Yes 2022 T -> A No 2099 G -> T Yes 2232 G -> A Yes 2261 G -> T Yes 2441 G -> A Yes 2637 C -> T Yes 2693 G -> T Yes 2833 C -> T Yes 2934 C -> T Yes 3249 A -> G No

Variant protein HUMPHOSLIP_PEA_(—)2_P30 (SEQ ID NO: 737) according to the present invention has an amino acid sequence as given at the end of the application; it is encoded by transcript(s) HUMPHOSLIP_PEA_(—)2_T6 (SEQ ID NO: 674). The location of the variant protein was determined according to results from a number of different software programs and analyses, including analyses from SignalP and other specialized programs. The variant protein is believed to be located as follows with regard to the cell: secreted. The protein localization is believed to be secreted because both signal-peptide prediction programs predict that this protein has a signal peptide, and neither trans-membrane region prediction program predicts that this protein has a trans-membrane region.

Variant protein HUMPHOSLIP_PEA_(—)2_P30 (SEQ ID NO: 737) also has the following non-silent SNPs (Single Nucleotide Polymorphisms) as listed in Table 12, (given according to their position(s) on the amino acid sequence, with the alternative amino acid(s) listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein HUMPHOSLIP_PEA_(—)2_P30 (SEQ ID NO: 737) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 12 Amino acid mutations SNP position(s) on amino acid sequence Alternative amino acid(s) Previously known SNP? 16 H -> R Yes 18 E -> V Yes 37 R -> Q Yes

Variant protein HUMPHOSLIP_PEA_(—)2_P30 (SEQ ID NO: 737) is encoded by the following transcript(s): HUMPHOSLIP_PEA_(—)2_T6 (SEQ ID NO: 674), for which the sequence(s) is/are given at the end of the application. The coding portion of transcript HUMPHOSLIP_PEA_(—)2_T6 (SEQ ID NO: 674) is shown in bold; this coding portion starts at position 276 and ends at position 431. The transcript also has the following SNPs as listed in Table 13 (given according to their position on the nucleotide sequence, with the alternative nucleic acid listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein HUMPHOSLIP_PEA_(—)2_P30 (SEQ ID NO: 737) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 13 Nucleic acid SNPs SNP position on nucleotide sequence Alternative nucleic acid Previously known SNP? 174 G -> T No 175 A -> T No 322 A -> G Yes 328 A -> T Yes 385 G -> A Yes 470 G -> C Yes 598 G -> A Yes 600 C -> A Yes 708 C -> No 708 C -> A No 790 C -> T Yes 852 C -> T Yes 867 T -> No 933 G -> No 933 G -> C No 962 A -> No 992 C -> No 1115 C -> No 1276 C -> T Yes 1336 G -> No 1367 C -> T Yes 1388 C -> No 1458 T -> A No 1469 C -> T Yes 1504 G -> C Yes 1561 T -> A Yes 1689 C -> A Yes 1708 C -> T No 1788 C -> T Yes 1804 A -> G No 1804 A -> T No 1869 A -> G Yes 1893 T -> A No 1970 G -> T Yes 2103 G -> A Yes 2132 G -> T Yes 2312 G -> A Yes 2508 C -> T Yes 2564 G -> T Yes 2704 C -> T Yes 2805 C -> T Yes 3120 A -> G No

Variant protein HUMPHOSLIP_PEA_(—)2_P31 (SEQ ID NO: 738) according to the present invention has an amino acid sequence as given at the end of the application; it is encoded by transcript(s) HUMPHOSLIP_PEA_(—)2_T7 (SEQ ID NO: 675). An alignment is given to the known protein (Phospholipid transfer protein precursor) at the end of the application. One or more alignments to one or more previously published protein sequences are given at the end of the application. A brief description of the relationship of the variant protein according to the present invention to each such aligned protein is as follows:

Comparison report between HUMPHOSLIP_PEA_(—)2_P31 (SEQ ID NO: 738) and PLTP_HUMAN:

1. An isolated chimeric polypeptide encoding for HUMPHOSLIP_PEA_(—)2_P31 (SEQ ID NO: 738), comprising a first amino acid sequence being at least 90% homologous to MALFGALFLALLAGAHAEFPGCKIRVTSKALELVKQEGLRFLEQELETITIPDLRGKEGH FYYNISE corresponding to amino acids 1-67 of PLTP_HUMAN, which also corresponds to amino acids 1-67 of HUMPHOSLIP_PEA_(—)2_P31 (SEQ ID NO: 738), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence PGLERGADKFPVVGGSSLFLALDLTLRPPVG (SEQ ID NO: 1085) corresponding to amino acids 68-98 of HUMPHOSLIP_PEA_(—)2_P31 (SEQ ID NO: 738), wherein said first amino acid sequence and second amino acid sequence are contiguous and in a sequential order.

2. An isolated polypeptide encoding for a tail of HUMPHOSLIP_PEA_(—)2_P31 (SEQ ID NO: 738), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence PGLERGADKFPVVGGSSLFLALDLTLRPPVG (SEQ ID NO: 1085) in HUMPHOSLIP_PEA_(—)2_P31 (SEQ ID NO: 738).

The location of the variant protein was determined according to results from a number of different software programs and analyses, including analyses from SignalP and other specialized programs. The variant protein is believed to be located as follows with regard to the cell: secreted. The protein localization is believed to be secreted because both signal-peptide prediction programs predict that this protein has a signal peptide, and neither trans-membrane region prediction program predicts that this protein has a trans-membrane region.

Variant protein HUMPHOSLIP_PEA_(—)2_P31 (SEQ ID NO: 738) also has the following non-silent SNPs (Single Nucleotide Polymorphisms) as listed in Table 14, (given according to their position(s) on the amino acid sequence, with the alternative amino acid(s) listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein HUMPHOSLIP_PEA_(—)2_P31 (SEQ ID NO: 738) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 14 Amino acid mutations SNP position(s) on amino acid sequence Alternative amino acid(s) Previously known SNP? 16 H -> R Yes 18 E -> V Yes

The glycosylation sites of variant protein HUMPHOSLIP_PEA_(—)2_P31 (SEQ ID NO: 738), as compared to the known protein Phospholipid transfer protein precursor, are described in Table 15 (given according to their position(s) on the amino acid sequence in the first column; the second column indicates whether the glycosylation site is present in the variant protein; and the last column indicates whether the position is different on the variant protein).

TABLE 15 Glycosylation site(s) Position(s) on known Present in Position in amino acid sequence variant protein? variant protein? 94 no 143 no 64 yes 64 245 no 398 no 117 no

Variant protein HUMPHOSLIP_PEA_(—)2_P31 (SEQ ID NO: 738) is encoded by the following transcript(s): HUMPHOSLIP_PEA_(—)2_T7 (SEQ ID NO: 675), for which the sequence(s) is/are given at the end of the application. The coding portion of transcript HUMPHOSLIP_PEA_(—)2_T7 (SEQ ID NO: 675) is shown in bold; this coding portion starts at position 276 and ends at position 569. The transcript also has the following SNPs as listed in Table 16 (given according to their position on the nucleotide sequence, with the alternative nucleic acid listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein HUMPHOSLIP_PEA_(—)2_P31 (SEQ ID NO: 738) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 16 Nucleic acid SNPs SNP position on nucleotide sequence Alternative nucleic acid Previously known SNP? 174 G -> T No 175 A -> T No 322 A -> G Yes 328 A -> T Yes 431 G -> A Yes 608 G -> C Yes 736 G -> A Yes 738 C -> A Yes 846 C -> No 846 C -> A No 928 C -> T Yes 990 C -> T Yes 1005 T -> No 1071 G -> No 1071 G -> C No 1100 A -> No 1130 C -> No 1253 C -> No 1414 C -> T Yes 1474 G -> No 1505 C -> T Yes 1526 C -> No 1596 T -> A No 1607 C -> T Yes 1642 G -> C Yes 1699 T -> A Yes 1827 C -> A Yes 1846 C -> T No 1926 C -> T Yes 1942 A -> G No 1942 A -> T No 2007 A -> G Yes 2031 T -> A No 2108 G -> T Yes 2241 G -> A Yes 2270 G -> T Yes 2450 G -> A Yes 2646 C -> T Yes 2702 G -> T Yes 2842 C -> T Yes 2943 C -> T Yes 3258 A -> G No

Variant protein HUMPHOSLIP_PEA_(—)2_P33 (SEQ ID NO: 739) according to the present invention has an amino acid sequence as given at the end of the application; it is encoded by transcript(s) HUMPHOSLIP_PEA_(—)2_T14 (SEQ ID NO: 676). An alignment is given to the known protein (Phospholipid transfer protein precursor) at the end of the application. One or more alignments to one or more previously published protein sequences are given at the end of the application. A brief description of the relationship of the variant protein according to the present invention to each such aligned protein is as follows:

Comparison report between HUMPHOSLIP_PEA_(—)2_P33 (SEQ ID NO: 739) and PLTP_HUMAN:

1. An isolated chimeric polypeptide encoding for HUMPHOSLIP_PEA_(—)2_P33 (SEQ ID NO: 739), comprising a first amino acid sequence being at least 90% homologous to MALFGALFLALLAGAHAEFPGCKIRVTSKALELVKQEGLRFLEQELETITIPDLRGKEGH FYYNISEVKVTELQLTSSELDFQPQQELMLQITNASLGLRFRRQLLYWFFYDGGYINAS AEGVSIRTGLELSRDPAGRMKVSNVSCQASVSRMHAAFGGTFKKVYDFLSTFITSGMRF LLNQQ corresponding to amino acids 1-183 of PLTP_HUMAN, which also corresponds to amino acids 1-183 of HUMPHOSLIP_PEA_(—)2_P33 (SEQ ID NO: 739), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence VWAATGRRVARVGMLSL (SEQ ID NO: 1086) corresponding to amino acids 184-200 of HUMPHOSLIP_PEA_(—)2_P33 (SEQ ID NO: 739), wherein said first amino acid sequence and second amino acid sequence are contiguous and in a sequential order.

2. An isolated polypeptide encoding for a tail of HUMPHOSLIP_PEA_(—)2_P33 (SEQ ID NO: 739), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence VWAATGRRVARVGMLSL (SEQ ID NO: 1086) in HUMPHOSLIP_PEA_(—)2_P33 (SEQ ID NO: 739).

The location of the variant protein was determined according to results from a number of different software programs and analyses, including analyses from SignalP and other specialized programs. The variant protein is believed to be located as follows with regard to the cell: secreted. The protein localization is believed to be secreted because both signal-peptide prediction programs predict that this protein has a signal peptide, and neither trans-membrane region prediction program predicts that this protein has a trans-membrane region.

Variant protein HUMPHOSLIP_PEA_(—)2_P33 (SEQ ID NO: 739) also has the following non-silent SNPs (Single Nucleotide Polymorphisms) as listed in Table 17, (given according to their position(s) on the amino acid sequence, with the alternative amino acid(s) listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein HUMPHOSLIP_PEA_(—)2_P33 (SEQ ID NO: 739) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 17 Amino acid mutations SNP position(s) on amino acid sequence Alternative amino acid(s) Previously known SNP? 16 H -> R Yes 18 E -> V Yes 81 D -> H Yes 124 S -> Y Yes 160 T -> No 160 T -> N No

The glycosylation sites of variant protein HUMPHOSLIP_PEA_(—)2_P33 (SEQ ID NO: 739), as compared to the known protein Phospholipid transfer protein precursor, are described in Table 18 (given according to their position(s) on the amino acid sequence in the first column; the second column indicates whether the glycosylation site is present in the variant protein; and the last column indicates whether the position is different on the variant protein).

TABLE 18 Glycosylation site(s) Position(s) on known amino Present in acid sequence variant protein? Position in variant protein? 94 yes 94 143 yes 143 64 yes 64 245 no 398 no 117 yes 117

Variant protein HUMPHOSLIP_PEA_(—)2_P33 (SEQ ID NO: 739) is encoded by the following transcript(s): HUMPHOSLIP_PEA_(—)2_T14 (SEQ ID NO: 676), for which the sequence(s) is/are given at the end of the application. The coding portion of transcript HUMPHOSLIP_PEA_(—)2_T14 (SEQ ID NO: 676) is shown in bold; this coding portion starts at position 276 and ends at position 875. The transcript also has the following SNPs as listed in Table 19 (given according to their position on the nucleotide sequence, with the alternative nucleic acid listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein HUMPHOSLIP_PEA_(—)2_P33 (SEQ ID NO: 739) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 19 Nucleic acid SNPs SNP position on nucleotide sequence Alternative nucleic acid Previously known SNP? 174 G -> T No 175 A -> T No 322 A -> G Yes 328 A -> T Yes 431 G -> A Yes 516 G -> C Yes 644 G -> A Yes 646 C -> A Yes 754 C -> No 754 C -> A No 921 C -> T Yes 983 C -> T Yes 998 T -> No 1064 G -> No 1064 G -> C No 1093 A -> No 1123 C -> No 1246 C -> No 1407 C -> T Yes 1467 G -> No 1498 C -> T Yes 1519 C -> No 1589 T -> A No 1600 C -> T Yes 1635 G -> C Yes 1692 T -> A Yes 1820 C -> A Yes 1839 C -> T No 1919 C -> T Yes 1935 A -> G No 1935 A -> T No 2000 A -> G Yes 2024 T -> A No 2101 G -> T Yes 2234 G -> A Yes 2263 G -> T Yes 2443 G -> A Yes 2639 C -> T Yes 2695 G -> T Yes 2835 C -> T Yes 2936 C -> T Yes 3251 A -> G No

Variant protein HUMPHOSLIP_PEA_(—)2_P34 (SEQ ID NO: 740) according to the present invention has an amino acid sequence as given at the end of the application; it is encoded by transcript(s) HUMPHOSLIP_PEA_(—)2_T16 (SEQ ID NO: 677). An alignment is given to the known protein (Phospholipid transfer protein precursor) at the end of the application. One or more alignments to one or more previously published protein sequences are given at the end of the application. A brief description of the relationship of the variant protein according to the present invention to each such aligned protein is as follows:

Comparison report between HUMPHOSLIP_PEA_(—)2_P34 (SEQ ID NO: 740) and PLTP_HUMAN:

1. An isolated chimeric polypeptide encoding for HUMPHOSLIP_PEA_(—)2_P34 (SEQ ID NO: 740), comprising a first amino acid sequence being at least 90% homologous to MALFGALFLALLAGAHAEFPGCKIRVTSKALELVKQEGLRFLEQELETITIPDLRGKEGH FYYNISEVKVTELQLTSSELDFQPQQELMLQITNASLGLRFRRQLLYWFFYDGGYINAS AEGVSIRTGLELSRDPAGRMKVSNVSCQASVSRMHAAFGGTFKKVYDFLSTFITSGMRF LLNQQICPVLYHAGTVLLNSLLDTVPV corresponding to amino acids 1-205 of PLTP_HUMAN, which also corresponds to amino acids 1-205 of HUMPHOSLIP_PEA_(—)2_P34 (SEQ ID NO: 740), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence LWTSLLALTIPS (SEQ ID NO: 1087) corresponding to amino acids 206-217 of HUMPHOSLIP_PEA_(—)2_P34 (SEQ ID NO: 740), wherein said first amino acid sequence and second amino acid sequence are contiguous and in a sequential order.

2. An isolated polypeptide encoding for a tail of HUMPHOSLIP_PEA_(—)2_P34 (SEQ ID NO: 740), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence LWTSLLALTIPS (SEQ ID NO: 1087) in HUMPHOSLIP_PEA_(—)2_P34 (SEQ ID NO: 740).

The location of the variant protein was determined according to results from a number of different software programs and analyses, including analyses from SignalP and other specialized programs. The variant protein is believed to be located as follows with regard to the cell: secreted. The protein localization is believed to be secreted because both signal-peptide prediction programs predict that this protein has a signal peptide, and neither trans-membrane region prediction program predicts that this protein has a trans-membrane region.

Variant protein HUMPHOSLIP_PEA_(—)2_P34 (SEQ ID NO: 740) also has the following non-silent SNPs (Single Nucleotide Polymorphisms) as listed in Table 20, (given according to their position(s) on the amino acid sequence, with the alternative amino acid(s) listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein HUMPHOSLIP_PEA_(—)2_P34 (SEQ ID NO: 740) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 20 Amino acid mutations SNP position(s) on amino acid sequence Alternative amino acid(s) Previously known SNP? 16 H -> R Yes 18 E -> V Yes 81 D -> H Yes 124 S -> Y Yes 160 T -> No 160 T -> N No 211 L -> No

The glycosylation sites of variant protein HUMPHOSLIP_PEA_(—)2_P34 (SEQ ID NO: 740), as compared to the known protein Phospholipid transfer protein precursor, are described in Table 21 (given according to their position(s) on the amino acid sequence in the first column; the second column indicates whether the glycosylation site is present in the variant protein; and the last column indicates whether the position is different on the variant protein).

TABLE 21 Glycosylation site(s) Position(s) on known amino Present in acid sequence variant protein? Position in variant protein? 94 yes 94 143 yes 143 64 yes 64 245 no 398 no 117 yes 117

Variant protein HUMPHOSLIP_PEA_(—)2_P34 (SEQ ID NO: 740) is encoded by the following transcript(s): HUMPHOSLIP_PEA_(—)2_T16 (SEQ ID NO: 677), for which the sequence(s) is/are given at the end of the application. The coding portion of transcript HUMPHOSLIP_PEA_(—)2_T16 (SEQ ID NO: 677) is shown in bold; this coding portion starts at position 276 and ends at position 926. The transcript also has the following SNPs as listed in Table 22 (given according to their position on the nucleotide sequence, with the alternative nucleic acid listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein HUMPHOSLIP_PEA_(—)2_P34 (SEQ ID NO: 740) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 22 Nucleic acid SNPs SNP position on nucleotide sequence Alternative nucleic acid Previously known SNP? 174 G -> T No 175 A -> T No 322 A -> G Yes 328 A -> T Yes 431 G -> A Yes 516 G -> C Yes 644 G -> A Yes 646 C -> A Yes 754 C -> No 754 C -> A No 836 C -> T Yes 891 C -> T Yes 906 T -> No 972 G -> No 972 G -> C No 1001 A -> No 1031 C -> No 1154 C -> No 1315 C -> T Yes 1375 G -> No 1406 C -> T Yes 1427 C -> No 1497 T -> A No 1508 C -> T Yes 1543 G -> C Yes 1600 T -> A Yes 1728 C -> A Yes 1747 C -> T No 1827 C -> T Yes 1843 A -> G No 1843 A -> T No 1908 A -> G Yes 1932 T -> A No 2009 G -> T Yes 2142 G -> A Yes 2171 G -> T Yes 2351 G -> A Yes 2547 C -> T Yes 2603 G -> T Yes 2743 C -> T Yes 2844 C -> T Yes 3159 A -> G No

Variant protein HUMPHOSLIP_PEA_(—)2_P35 (SEQ ID NO: 741) according to the present invention has an amino acid sequence as given at the end of the application; it is encoded by transcript(s) HUMPHOSLIP_PEA_(—)2_T18 (SEQ ID NO: 679). An alignment is given to the known protein (Phospholipid transfer protein precursor) at the end of the application. One or more alignments to one or more previously published protein sequences are given at the end of the application. A brief description of the relationship of the variant protein according to the present invention to each such aligned protein is as follows:

Comparison report between HUMPHOSLIP_PEA_(—)2_P35 (SEQ ID NO: 741) and PLTP_HUMAN:

1. An isolated chimeric polypeptide encoding for HUMPHOSLIP_PEA_(—)2_P35 (SEQ ID NO: 741), comprising a first amino acid sequence being at least 90% homologous to MALFGALFLALLAGAHAEFPGCKIRVTSKALELVKQEGLRFLEQELETITIPDLRGKEGH FYYNISEVKVTELQLTSSELDFQPQQELMLQITNASLGLRFRRQLLYWF corresponding to amino acids 1-109 of PLTP_HUMAN, which also corresponds to amino acids 1-109 of HUMPHOSLIP_PEA_(—)2_P35 (SEQ ID NO: 741), a second amino acid sequence bridging amino acid sequence comprising of L, a third amino acid sequence being at least 90% homologous to KVYDFLSTFITSGMRFLLNQQ corresponding to amino acids 163-183 of PLTP_HUMAN, which also corresponds to amino acids 111-131 of HUMPHOSLIP_PEA_(—)2_P35 (SEQ ID NO: 741), and a fourth amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence VWAATGRRVARVGMLSL (SEQ ID NO: 1086) corresponding to amino acids 132-148 of HUMPHOSLIP_PEA_(—)2_P35 (SEQ ID NO: 741), wherein said first amino acid sequence, second amino acid sequence, third amino acid sequence and fourth amino acid sequence are contiguous and in a sequential order.

2. An isolated polypeptide encoding for an edge portion of HUMPHOSLIP_PEA_(—)2_P35 (SEQ ID NO: 741), comprising a polypeptide having a length “n”, wherein n is at least about 10 amino acids in length, optionally at least about 20 amino acids in length, preferably at least about 30 amino acids in length, more preferably at least about 40 amino acids in length and most preferably at least about 50 amino acids in length, wherein at least two amino acids comprise FLK having a structure as follows (numbering according to HUMPHOSLIP_PEA_(—)2_P35 (SEQ ID NO: 741)): a sequence starting from any of amino acid numbers 109-x to 109; and ending at any of amino acid numbers 111+((n−2)−x), in which x varies from 0 to n−2.

3. An isolated polypeptide encoding for a tail of HUMPHOSLIP_PEA_(—)2_P35 (SEQ ID NO: 741), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence VWAATGRRVARVGMLSL (SEQ ID NO: 1086) in HUMPHOSLIP_PEA_(—)2_P35 (SEQ ID NO: 741).

The location of the variant protein was determined according to results from a number of different software programs and analyses, including analyses from SignalP and other specialized programs. The variant protein is believed to be located as follows with regard to the cell: secreted. The protein localization is believed to be secreted because both signal-peptide prediction programs predict that this protein has a signal peptide, and neither trans-membrane region prediction program predicts that this protein has a trans-membrane region.

Variant protein HUMPHOSLIP_PEA_(—)2_P35 (SEQ ID NO: 741) also has the following non-silent SNPs (Single Nucleotide Polymorphisms) as listed in Table 23, (given according to their position(s) on the amino acid sequence, with the alternative amino acid(s) listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein HUMPHOSLIP_PEA_(—)2_P35 (SEQ ID NO: 741) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 23 Amino acid mutations SNP position(s) on amino acid sequence Alternative amino acid(s) Previously known SNP? 16 H -> R Yes 18 E -> V Yes 81 D -> H Yes

The glycosylation sites of variant protein HUMPHOSLIP_PEA_(—)2_P35 (SEQ ID NO: 741), as compared to the known protein Phospholipid transfer protein precursor, are described in Table 24 (given according to their position(s) on the amino acid sequence in the first column; the second column indicates whether the glycosylation site is present in the variant protein; and the last column indicates whether the position is different on the variant protein).

TABLE 24 Glycosylation site(s) Position(s) on known amino Present in acid sequence variant protein? Position in variant protein? 94 yes 94 143 no 64 yes 64 245 no 398 no 117 no

Variant protein HUMPHOSLIP_PEA_(—)2_P35 (SEQ ID NO: 741) is encoded by the following transcript(s): HUMPHOSLIP_PEA_(—)2_T18 (SEQ ID NO: 679), for which the sequence(s) is/are given at the end of the application. The coding portion of transcript HUMPHOSLIP_PEA_(—)2_T18 (SEQ ID NO: 679) is shown in bold; this coding portion starts at position 276 and ends at position 719. The transcript also has the following SNPs as listed in Table 25 (given according to their position on the nucleotide sequence, with the alternative nucleic acid listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein HUMPHOSLIP_PEA_(—)2_P35 (SEQ ID NO: 741) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 25 Nucleic acid SNPs SNP position on nucleotide sequence Alternative nucleic acid Previously known SNP? 174 G -> T No 175 A -> T No 322 A -> G Yes 328 A -> T Yes 431 G -> A Yes 516 G -> C Yes 765 C -> T Yes 827 C -> T Yes 842 T -> No 908 G -> No 908 G -> C No 937 A -> No 967 C -> No 1090 C -> No 1251 C -> T Yes 1311 G -> No 1342 C -> T Yes 1363 C -> No 1433 T -> A No 1444 C -> T Yes 1479 G -> C Yes 1536 T -> A Yes 1664 C -> A Yes 1683 C -> T No 1763 C -> T Yes 1779 A -> G No 1779 A -> T No 1844 A -> G Yes 1868 T -> A No 1945 G -> T Yes 2078 G -> A Yes 2107 G -> T Yes 2287 G -> A Yes 2483 C -> T Yes 2539 G -> T Yes 2679 C -> T Yes 2780 C -> T Yes 3095 A -> G No

As noted above, cluster HUMPHOSLIP features 53 segment(s), which were listed in Table 2 above and for which the sequence(s) are given at the end of the application. These segment(s) are portions of nucleic acid sequence(s) which are described herein separately because they are of particular interest. A description of each segment according to the present invention is now provided.

Segment cluster HUMPHOSLIP_PEA_(—)2_node_(—)0 (SEQ ID NO: 681) according to the present invention is supported by 150 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HUMPHOSLIP_PEA_(—)2_T6 (SEQ ID NO: 674), HUMPHOSLIP_PEA_(—)2_T7 (SEQ ID NO: 675), HUMPHOSLIP_PEA_(—)2_T14 (SEQ ID NO: 676), HUMPHOSLIP_PEA_(—)2_T16 (SEQ ID NO: 677), HUMPHOSLIP_PEA_(—)2_T17 (SEQ ID NO: 678), HUMPHOSLIP_PEA_(—)2_T18 (SEQ ID NO: 679) and HUMPHOSLIP_PEA_(—)2_T19 (SEQ ID NO: 680). Table 26 below describes the starting and ending position of this segment on each transcript.

TABLE 26 Segment location on transcripts Segment Segment starting ending Transcript name position position HUMPHOSLIP_PEA_2_T6 1 264 (SEQ ID NO: 674) HUMPHOSLIP_PEA_2_T7 1 264 (SEQ ID NO: 675) HUMPHOSLIP_PEA_2_T14 1 264 (SEQ ID NO: 676) HUMPHOSLIP_PEA_2_T16 1 264 (SEQ ID NO: 677) HUMPHOSLIP_PEA_2_T17 1 264 (SEQ ID NO: 678) HUMPHOSLIP_PEA_2_T18 1 264 (SEQ ID NO: 679) HUMPHOSLIP_PEA_2_T19 1 264 (SEQ ID NO: 680)

Segment cluster HUMPHOSLIP_PEA_(—)2_node_(—)19_ (SEQ ID NO: 682) according to the present invention is supported by 186 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HUMPHOSLIP_PEA_(—)2_T6 (SEQ ID NO: 674), HUMPHOSLIP_PEA_(—)2_T7 (SEQ ID NO: 675), HUMPHOSLIP_PEA_(—)2_T14 (SEQ ID NO: 676), HUMPHOSLIP_PEA_(—)2_T16 (SEQ ID NO: 677) and HUMPHOSLIP_PEA_(—)2_T19 (SEQ ID NO: 680). Table 27 below describes the starting and ending position of this segment on each transcript.

TABLE 27 Segment location on transcripts Segment Segment starting ending Transcript name position position HUMPHOSLIP_PEA_2_T6 559 714 (SEQ ID NO: 674) HUMPHOSLIP_PEA_2_T7 697 852 (SEQ ID NO: 675) HUMPHOSLIP_PEA_2_T14 605 760 (SEQ ID NO: 676) HUMPHOSLIP_PEA_2_T16 605 760 (SEQ ID NO: 677) HUMPHOSLIP_PEA_2_T19 605 760 (SEQ ID NO: 680)

Segment cluster HUMPHOSLIP_PEA_(—)2_node_(—)34 (SEQ ID NO: 683) according to the present invention is supported by 191 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HUMPHOSLIP_PEA_(—)2_T6 (SEQ ID NO: 674), HUMPHOSLIP_PEA_(—)2_T7 (SEQ ID NO: 675), HUMPHOSLIP_PEA_(—)2_T14 (SEQ ID NO: 676), HUMPHOSLIP_PEA_(—)2_T16 (SEQ ID NO: 677), HUMPHOSLIP_PEA_(—)2_T17 (SEQ ID NO: 678), HUMPHOSLIP_PEA_(—)2_T18 (SEQ ID NO: 679) and HUMPHOSLIP_PEA_(—)2_T19 (SEQ ID NO: 680). Table 28 below describes the starting and ending position of this segment on each transcript.

TABLE 28 Segment location on transcripts Segment Segment starting ending Transcript name position position HUMPHOSLIP_PEA_2_T6 971 1111 (SEQ ID NO: 674) HUMPHOSLIP_PEA_2_T7 1109 1249 (SEQ ID NO: 675) HUMPHOSLIP_PEA_2_T14 1102 1242 (SEQ ID NO: 676) HUMPHOSLIP_PEA_2_T16 1010 1150 (SEQ ID NO: 677) HUMPHOSLIP_PEA_2_T17 732 872 (SEQ ID NO: 678) HUMPHOSLIP_PEA_2_T18 946 1086 (SEQ ID NO: 679) HUMPHOSLIP_PEA_2_T19 1017 1157 (SEQ ID NO: 680)

Segment cluster HUMPHOSLIP_PEA_(—)2_node_(—)68 (SEQ ID NO: 684) according to the present invention is supported by 131 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HUMPHOSLIP_PEA_(—)2_T6 (SEQ ID NO: 674), HUMPHOSLIP_PEA_(—)2_T7 (SEQ ID NO: 675), HUMPHOSLIP_PEA_(—)2_T14 (SEQ ID NO: 676), HUMPHOSLIP_PEA_(—)2_T16 (SEQ ID NO: 677), HUMPHOSLIP_PEA_(—)2_T17 (SEQ ID NO: 678), HUMPHOSLIP_PEA_(—)2_T18 (SEQ ID NO: 679) and HUMPHOSLIP_PEA_(—)2_T19 (SEQ ID NO: 680). Table 29 below describes the starting and ending position of this segment on each transcript.

TABLE 29 Segment location on transcripts Segment Segment starting ending Transcript name position position HUMPHOSLIP_PEA_2_T6 1867 2285 (SEQ ID NO: 674) HUMPHOSLIP_PEA_2_T7 2005 2423 (SEQ ID NO: 675) HUMPHOSLIP_PEA_2_T14 1998 2416 (SEQ ID NO: 676) HUMPHOSLIP_PEA_2_T16 1906 2324 (SEQ ID NO: 677) HUMPHOSLIP_PEA_2_T17 1628 2046 (SEQ ID NO: 678) HUMPHOSLIP_PEA_2_T18 1842 2260 (SEQ ID NO: 679) HUMPHOSLIP_PEA_2_T19 1996 2414 (SEQ ID NO: 680)

Segment cluster HUMPHOSLIP_PEA_(—)2_node_(—)70 (SEQ ID NO: 685) according to the present invention is supported by 5 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HUMPHOSLIP_PEA_(—)2_T6 (SEQ ID NO: 674), HUMPHOSLIP_PEA_(—)2_T7 (SEQ ID NO: 675), HUMPHOSLIP_PEA_(—)2_T14 (SEQ ID NO: 676), HUMPHOSLIP_PEA_(—)2_T16 (SEQ ID NO: 677), HUMPHOSLIP_PEA_(—)2_T17 (SEQ ID NO: 678), HUMPHOSLIP_PEA_(—)2_T18 (SEQ ID NO: 679) and HUMPHOSLIP_PEA_(—)2_T19 (SEQ ID NO: 680). Table 30 below describes the starting and ending position of this segment on each transcript.

TABLE 30 Segment location on transcripts Segment Segment starting ending Transcript name position position HUMPHOSLIP_PEA_2_T6 2298 2529 (SEQ ID NO: 674) HUMPHOSLIP_PEA_2_T7 2436 2667 (SEQ ID NO: 675) HUMPHOSLIP_PEA_2_T14 2429 2660 (SEQ ID NO: 676) HUMPHOSLIP_PEA_2_T16 2337 2568 (SEQ ID NO: 677) HUMPHOSLIP_PEA_2_T17 2059 2290 (SEQ ID NO: 678) HUMPHOSLIP_PEA_2_T18 2273 2504 (SEQ ID NO: 679) HUMPHOSLIP_PEA_2_T19 2427 2658 (SEQ ID NO: 680)

Segment cluster HUMPHOSLIP_PEA_(—)2_node_(—)75 (SEQ ID NO: 686) according to the present invention is supported by 14 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HUMPHOSLIP_PEA_(—)2_T6 (SEQ ID NO: 674), HUMPHOSLIP_PEA_(—)2_T7 (SEQ ID NO: 675), HUMPHOSLIP_PEA_(—)2_T14 (SEQ ID NO: 676), HUMPHOSLIP_PEA_(—)2_T16 (SEQ ID NO: 677), HUMPHOSLIP_PEA_(—)2_T17 (SEQ ID NO: 678), HUMPHOSLIP_PEA_(—)2_T18 (SEQ ID NO: 679) and HUMPHOSLIP_PEA_(—)2_T19 (SEQ ID NO: 680). Table 31 below describes the starting and ending position of this segment on each transcript.

TABLE 31 Segment location on transcripts Segment Segment starting ending Transcript name position position HUMPHOSLIP_PEA_2_T6 2846 3125 (SEQ ID NO: 674) HUMPHOSLIP_PEA_2_T7 2984 3263 (SEQ ID NO: 675) HUMPHOSLIP_PEA_2_T14 2977 3256 (SEQ ID NO: 676) HUMPHOSLIP_PEA_2_T16 2885 3164 (SEQ ID NO: 677) HUMPHOSLIP_PEA_2_T17 2607 2886 (SEQ ID NO: 678) HUMPHOSLIP_PEA_2_T18 2821 3100 (SEQ ID NO: 679) HUMPHOSLIP_PEA_2_T19 2975 3254 (SEQ ID NO: 680)

According to an optional embodiment of the present invention, short segments related to the above cluster are also provided. These segments are up to about 120 bp in length, and so are included in a separate description.

Segment cluster HUMPHOSLIP_PEA_(—)2_node_(—)2 (SEQ ID NO: 687) according to the present invention is supported by 159 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HUMPHOSLIP_PEA_(—)2_T6 (SEQ ID NO: 674), HUMPHOSLIP_PEA_(—)2_T7 (SEQ ID NO: 675), HUMPHOSLIP_PEA_(—)2_T14 (SEQ ID NO: 676), HUMPHOSLIP_PEA_(—)2_T16 (SEQ ID NO: 677), HUMPHOSLIP_PEA_(—)2_T17 (SEQ ID NO: 678), HUMPHOSLIP_PEA_(—)2_T18 (SEQ ID NO: 679) and HUMPHOSLIP_PEA_(—)2_T19 (SEQ ID NO: 680). Table 32 below describes the starting and ending position of this segment on each transcript.

TABLE 32 Segment location on transcripts Segment Segment starting ending Transcript name position position HUMPHOSLIP_PEA_2_T6 265 337 (SEQ ID NO: 674) HUMPHOSLIP_PEA_2_T7 265 337 (SEQ ID NO: 675) HUMPHOSLIP_PEA_2_T14 265 337 (SEQ ID NO: 676) HUMPHOSLIP_PEA_2_T16 265 337 (SEQ ID NO: 677) HUMPHOSLIP_PEA_2_T17 265 337 (SEQ ID NO: 678) HUMPHOSLIP_PEA_2_T18 265 337 (SEQ ID NO: 679) HUMPHOSLIP_PEA_2_T19 265 337 (SEQ ID NO: 680)

Segment cluster HUMPHOSLIP_PEA_(—)2_node_(—)3 (SEQ ID NO: 688) according to the present invention can be found in the following transcript(s): HUMPHOSLIP_PEA_(—)2_T7 (SEQ ID NO: 675), HUMPHOSLIP_PEA_(—)2_T14 (SEQ ID NO: 676), HUMPHOSLIP_PEA_(—)2_T16 (SEQ ID NO: 677), HUMPHOSLIP_PEA_(—)2_T17 (SEQ ID NO: 678), HUMPHOSLIP_PEA_(—)2_T18 (SEQ ID NO: 679) and HUMPHOSLIP_PEA_(—)2_T19 (SEQ ID NO: 680). Table 33 below describes the starting and ending position of this segment on each transcript.

TABLE 33 Segment location on transcripts Segment Segment starting ending Transcript name position position HUMPHOSLIP_PEA_2_T7 338 355 (SEQ ID NO: 675) HUMPHOSLIP_PEA_2_T14 338 355 (SEQ ID NO: 676) HUMPHOSLIP_PEA_2_T16 338 355 (SEQ ID NO: 677) HUMPHOSLIP_PEA_2_T17 338 355 (SEQ ID NO: 678) HUMPHOSLIP_PEA_2_T18 338 355 (SEQ ID NO: 679) HUMPHOSLIP_PEA_2_T19 338 355 (SEQ ID NO: 680)

Segment cluster HUMPHOSLIP_PEA_(—)2_node_(—)4 (SEQ ID NO: 689) according to the present invention can be found in the following transcript(s): HUMPHOSLIP_PEA_(—)2_T7 (SEQ ID NO: 675), HUMPHOSLIP_PEA_(—)2_T14 (SEQ ID NO: 676), HUMPHOSLIP_PEA_(—)2_T16 (SEQ ID NO: 677), HUMPHOSLIP_PEA_(—)2_T17 (SEQ ID NO: 678), HUMPHOSLIP_PEA_(—)2_T18 (SEQ ID NO: 679) and HUMPHOSLIP_PEA_(—)2_T19 (SEQ ID NO: 680). Table 34 below describes the starting and ending position of this segment on each transcript.

TABLE 34 Segment location on transcripts Segment Segment starting ending Transcript name position position HUMPHOSLIP_PEA_2_T7 356 375 (SEQ ID NO: 675) HUMPHOSLIP_PEA_2_T14 356 375 (SEQ ID NO: 676) HUMPHOSLIP_PEA_2_T16 356 375 (SEQ ID NO: 677) HUMPHOSLIP_PEA_2_T17 356 375 (SEQ ID NO: 678) HUMPHOSLIP_PEA_2_T18 356 375 (SEQ ID NO: 679) HUMPHOSLIP_PEA_2_T19 356 375 (SEQ ID NO: 680)

Segment cluster HUMPHOSLIP_PEA_(—)2_node_(—)6 (SEQ ID NO: 690) according to the present invention can be found in the following transcript(s): HUMPHOSLIP_PEA_(—)2_T7 (SEQ ID NO: 675), HUMPHOSLIP_PEA_(—)2_T14 (SEQ ID NO: 676), HUMPHOSLIP_PEA_(—)2_T16 (SEQ ID NO: 677), HUMPHOSLIP_PEA_(—)2_T17 (SEQ ID NO: 678), HUMPHOSLIP_PEA_(—)2_T18 (SEQ ID NO: 679) and HUMPHOSLIP_PEA_(—)2_T19 (SEQ ID NO: 680). Table 35 below describes the starting and ending position of this segment on each transcript.

TABLE 35 Segment location on transcripts Segment Segment starting ending Transcript name position position HUMPHOSLIP_PEA_2_T7 376 383 (SEQ ID NO: 675) HUMPHOSLIP_PEA_2_T14 376 383 (SEQ ID NO: 676) HUMPHOSLIP_PEA_2_T16 376 383 (SEQ ID NO: 677) HUMPHOSLIP_PEA_2_T17 376 383 (SEQ ID NO: 678) HUMPHOSLIP_PEA_2_T18 376 383 (SEQ ID NO: 679) HUMPHOSLIP_PEA_2_T19 376 383 (SEQ ID NO: 680)

Segment cluster HUMPHOSLIP_PEA_(—)2_node_(—)7 (SEQ ID NO: 691) according to the present invention can be found in the following transcript(s): HUMPHOSLIP_PEA_(—)2_T6 (SEQ ID NO: 674), HUMPHOSLIP_PEA_(—)2_T7 (SEQ ID NO: 675), HUMPHOSLIP_PEA_(—)2_T14 (SEQ ID NO: 676), HUMPHOSLIP_PEA_(—)2_T16 (SEQ ID NO: 677), HUMPHOSLIP_PEA_(—)2_T17 (SEQ ID NO: 678), HUMPHOSLIP_PEA_(—)2_T18 (SEQ ID NO: 679) and HUMPHOSLIP_PEA_(—)2_T19 (SEQ ID NO: 680). Table 36 below describes the starting and ending position of this segment on each transcript.

TABLE 36 Segment location on transcripts Segment Segment starting ending Transcript name position position HUMPHOSLIP_PEA_2_T6 338 343 (SEQ ID NO: 674) HUMPHOSLIP_PEA_2_T7 384 389 (SEQ ID NO: 675) HUMPHOSLIP_PEA_2_T14 384 389 (SEQ ID NO: 676) HUMPHOSLIP_PEA_2_T16 384 389 (SEQ ID NO: 677) HUMPHOSLIP_PEA_2_T17 384 389 (SEQ ID NO: 678) HUMPHOSLIP_PEA_2_T18 384 389 (SEQ ID NO: 679) HUMPHOSLIP_PEA_2_T19 384 389 (SEQ ID NO: 680)

Segment cluster HUMPHOSLIP_PEA_(—)2_node_(—)8 (SEQ ID NO: 692) according to the present invention is supported by 171 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HUMPHOSLIP_PEA_(—)2_T6 (SEQ ID NO: 674), HUMPHOSLIP_PEA_(—)2_T7 (SEQ ID NO: 675), HUMPHOSLIP_PEA_(—)2_T14 (SEQ ID NO: 676), HUMPHOSLIP_PEA_(—)2_T16 (SEQ ID NO: 677), HUMPHOSLIP_PEA_(—)2_T17 (SEQ ID NO: 678), HUMPHOSLIP_PEA_(—)2_T18 (SEQ ID NO: 679) and HUMPHOSLIP_PEA_(—)2_T19 (SEQ ID NO: 680). Table 37 below describes the starting and ending position of this segment on each transcript.

TABLE 37 Segment location on transcripts Segment Segment starting ending Transcript name position position HUMPHOSLIP_PEA_2_T6 344 378 (SEQ ID NO: 674) HUMPHOSLIP_PEA_2_T7 390 424 (SEQ ID NO: 675) HUMPHOSLIP_PEA_2_T14 390 424 (SEQ ID NO: 676) HUMPHOSLIP_PEA_2_T16 390 424 (SEQ ID NO: 677) HUMPHOSLIP_PEA_2_T17 390 424 (SEQ ID NO: 678) HUMPHOSLIP_PEA_2_T18 390 424 (SEQ ID NO: 679) HUMPHOSLIP_PEA_2_T19 390 424 (SEQ ID NO: 680)

Segment cluster HUMPHOSLIP_PEA_(—)2_node_(—)9 (SEQ ID NO: 693) according to the present invention is supported by 168 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HUMPHOSLIP_PEA_(—)2_T6 (SEQ ID NO: 674), HUMPHOSLIP_PEA_(—)2_T7 (SEQ ID NO: 675), HUMPHOSLIP_PEA_(—)2_T14 (SEQ ID NO: 676), HUMPHOSLIP_PEA_(—)2_T16 (SEQ ID NO: 677), HUMPHOSLIP_PEA_(—)2_T17 (SEQ ID NO: 678), HUMPHOSLIP_PEA_(—)2_T18 (SEQ ID NO: 679) and HUMPHOSLIP_PEA_(—)2_T19 (SEQ ID NO: 680). Table 38 below describes the starting and ending position of this segment on each transcript.

TABLE 38 Segment location on transcripts Segment Segment starting ending Transcript name position position HUMPHOSLIP_PEA_2_T6 379 429 (SEQ ID NO: 674) HUMPHOSLIP_PEA_2_T7 425 475 (SEQ ID NO: 675) HUMPHOSLIP_PEA_2_T14 425 475 (SEQ ID NO: 676) HUMPHOSLIP_PEA_2_T16 425 475 (SEQ ID NO: 677) HUMPHOSLIP_PEA_2_T17 425 475 (SEQ ID NO: 678) HUMPHOSLIP_PEA_2_T18 425 475 (SEQ ID NO: 679) HUMPHOSLIP_PEA_2_T19 425 475 (SEQ ID NO: 680)

Segment cluster HUMPHOSLIP_PEA_(—)2_node_(—)14 (SEQ ID NO: 694) according to the present invention is supported by 6 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HUMPHOSLIP_PEA_(—)2_T7 (SEQ ID NO: 675). Table 39 below describes the starting and ending position of this segment on each transcript.

TABLE 39 Segment location on transcripts Segment Segment starting ending Transcript name position position HUMPHOSLIP_PEA_2_T7 476 567 (SEQ ID NO: 675)

Segment cluster HUMPHOSLIP_PEA_(—)2_node_(—)15 (SEQ ID NO: 695) according to the present invention can be found in the following transcript(s): HUMPHOSLIP_PEA_(—)2_T6 (SEQ ID NO: 674), HUMPHOSLIP_PEA_(—)2_T7 (SEQ ID NO: 675), HUMPHOSLIP_PEA_(—)2_T14 (SEQ ID NO: 676), HUMPHOSLIP_PEA_(—)2_T16 (SEQ ID NO: 677), HUMPHOSLIP_PEA_(—)2_T18 (SEQ ID NO: 679) and HUMPHOSLIP_PEA_(—)2_T19 (SEQ ID NO: 680). Table 40 below describes the starting and ending position of this segment on each transcript.

TABLE 40 Segment location on transcripts Segment Segment starting ending Transcript name position position HUMPHOSLIP_PEA_2_T6 430 445 (SEQ ID NO: 674) HUMPHOSLIP_PEA_2_T7 568 583 (SEQ ID NO: 675) HUMPHOSLIP_PEA_2_T14 476 491 (SEQ ID NO: 676) HUMPHOSLIP_PEA_2_T16 476 491 (SEQ ID NO: 677) HUMPHOSLIP_PEA_2_T18 476 491 (SEQ ID NO: 679) HUMPHOSLIP_PEA_2_T19 476 491 (SEQ ID NO: 680)

Segment cluster HUMPHOSLIP_PEA_(—)2_node_(—)16 (SEQ ID NO: 696) according to the present invention is supported by 179 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HUMPHOSLIP_PEA_(—)2_T6 (SEQ ID NO: 674), HUMPHOSLIP_PEA_(—)2_T7 (SEQ ID NO: 675), HUMPHOSLIP_PEA_(—)2_T14 (SEQ ID NO: 676), HUMPHOSLIP_PEA_(—)2_T16 (SEQ ID NO: 677), HUMPHOSLIP_PEA_(—)2_T18 (SEQ ID NO: 679) and HUMPHOSLIP_PEA_(—)2_T19 (SEQ ID NO: 680). Table 41 below describes the starting and ending position of this segment on each transcript.

TABLE 41 Segment location on transcripts Segment Segment starting ending Transcript name position position HUMPHOSLIP_PEA_2_T6 446 534 (SEQ ID NO: 674) HUMPHOSLIP_PEA_2_T7 584 672 (SEQ ID NO: 675) HUMPHOSLIP_PEA_2_T14 492 580 (SEQ ID NO: 676) HUMPHOSLIP_PEA_2_T16 492 580 (SEQ ID NO: 677) HUMPHOSLIP_PEA_2_T18 492 580 (SEQ ID NO: 679) HUMPHOSLIP_PEA_2_T19 492 580 (SEQ ID NO: 680)

Segment cluster HUMPHOSLIP_PEA_(—)2_node_(—)17 (SEQ ID NO: 697) according to the present invention can be found in the following transcript(s): HUMPHOSLIP_PEA_(—)2_T6 (SEQ ID NO: 674), HUMPHOSLIP_PEA_(—)2_T7 (SEQ ID NO: 675), HUMPHOSLIP_PEA_(—)2_T14 (SEQ ID NO: 676), HUMPHOSLIP_PEA_(—)2_T16 (SEQ ID NO: 677), HUMPHOSLIP_PEA_(—)2_T18 (SEQ ID NO: 679) and HUMPHOSLIP_PEA_(—)2_T19 (SEQ ID NO: 680). Table 42 below describes the starting and ending position of this segment on each transcript.

TABLE 42 Segment location on transcripts Segment Segment starting ending Transcript name position position HUMPHOSLIP_PEA_2_T6 535 558 (SEQ ID NO: 674) HUMPHOSLIP_PEA_2_T7 673 696 (SEQ ID NO: 675) HUMPHOSLIP_PEA_2_T14 581 604 (SEQ ID NO: 676) HUMPHOSLIP_PEA_2_T16 581 604 (SEQ ID NO: 677) HUMPHOSLIP_PEA_2_T18 581 604 (SEQ ID NO: 679) HUMPHOSLIP_PEA_2_T19 581 604 (SEQ ID NO: 680)

Segment cluster HUMPHOSLIP_PEA_(—)2_node_(—)23 (SEQ ID NO: 698) according to the present invention is supported by 168 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HUMPHOSLIP_PEA_(—)2_T6 (SEQ ID NO: 674), HUMPHOSLIP_PEA_(—)2_T7 (SEQ ID NO: 675), HUMPHOSLIP_PEA_(—)2_T14 (SEQ ID NO: 676), HUMPHOSLIP_PEA_(—)2_T16 (SEQ ID NO: 677), HUMPHOSLIP_PEA_(—)2_T17 (SEQ ID NO: 678), HUMPHOSLIP_PEA_(—)2_T18 (SEQ ID NO: 679) and HUMPHOSLIP_PEA_(—)2_T19 (SEQ ID NO: 680). Table 43 below describes the starting and ending position of this segment on each transcript.

TABLE 43 Segment location on transcripts Segment Segment starting ending Transcript name position position HUMPHOSLIP_PEA_2_T6 715 766 (SEQ ID NO: 674) HUMPHOSLIP_PEA_2_T7 853 904 (SEQ ID NO: 675) HUMPHOSLIP_PEA_2_T14 761 812 (SEQ ID NO: 676) HUMPHOSLIP_PEA_2_T16 761 812 (SEQ ID NO: 677) HUMPHOSLIP_PEA_2_T17 476 527 (SEQ ID NO: 678) HUMPHOSLIP_PEA_2_T18 605 656 (SEQ ID NO: 679) HUMPHOSLIP_PEA_2_T19 761 812 (SEQ ID NO: 680)

Segment cluster HUMPHOSLIP_PEA_(—)2_node_(—)24 (SEQ ID NO: 699) according to the present invention can be found in the following transcript(s): HUMPHOSLIP_PEA_(—)2_T6 (SEQ ID NO: 674), HUMPHOSLIP_PEA_(—)2_T7 (SEQ ID NO: 675), HUMPHOSLIP_PEA_(—)2_T14 (SEQ ID NO: 676), HUMPHOSLIP_PEA_(—)2_T16 (SEQ ID NO: 677), HUMPHOSLIP_PEA_(—)2_T17 (SEQ ID NO: 678), HUMPHOSLIP_PEA_(—)2_T18 (SEQ ID NO: 679) and HUMPHOSLIP_PEA_(—)2_T19 (SEQ ID NO: 680). Table 44 below describes the starting and ending position of this segment on each transcript.

TABLE 44 Segment location on transcripts Segment Segment starting ending Transcript name position position HUMPHOSLIP_PEA_2_T6 767 778 (SEQ ID NO: 674) HUMPHOSLIP_PEA_2_T7 905 916 (SEQ ID NO: 675) HUMPHOSLIP_PEA_2_T14 813 824 (SEQ ID NO: 676) HUMPHOSLIP_PEA_2_T16 813 824 (SEQ ID NO: 677) HUMPHOSLIP_PEA_2_T17 528 539 (SEQ ID NO: 678) HUMPHOSLIP_PEA_2_T18 657 668 (SEQ ID NO: 679) HUMPHOSLIP_PEA_2_T19 813 824 (SEQ ID NO: 680)

Segment cluster HUMPHOSLIP_PEA_(—)2_node_(—)25 (SEQ ID NO: 700) according to the present invention is supported by 5 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HUMPHOSLIP_PEA_(—)2_T14 (SEQ ID NO: 676) and HUMPHOSLIP_PEA_(—)2_T18 (SEQ ID NO: 679). Table 45 below describes the starting and ending position of this segment on each transcript.

TABLE 45 Segment location on transcripts Segment Segment starting ending Transcript name position position HUMPHOSLIP_PEA_2_T14 825 909 (SEQ ID NO: 676) HUMPHOSLIP_PEA_2_T18 669 753 (SEQ ID NO: 679)

Segment cluster HUMPHOSLIP_PEA_(—)2_node_(—)26 (SEQ ID NO: 701) according to the present invention is supported by 163 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HUMPHOSLIP_PEA_(—)2_T6 (SEQ ID NO: 674), HUMPHOSLIP_PEA_(—)2_T7 (SEQ ID NO: 675), HUMPHOSLIP_PEA_(—)2_T14 (SEQ ID NO: 676), HUMPHOSLIP_PEA_(—)2_T16 (SEQ ID NO: 677), HUMPHOSLIP_PEA_(—)2_T17 (SEQ ID NO: 678), HUMPHOSLIP_PEA_(—)2_T18 (SEQ ID NO: 679) and HUMPHOSLIP_PEA_(—)2_T19 (SEQ ID NO: 680). Table 46 below describes the starting and ending position of this segment on each transcript.

TABLE 46 Segment location on transcripts Segment Segment starting ending Transcript name position position HUMPHOSLIP_PEA_2_T6 779 842 (SEQ ID NO: 674) HUMPHOSLIP_PEA_2_T7 917 980 (SEQ ID NO: 675) HUMPHOSLIP_PEA_2_T14 910 973 (SEQ ID NO: 676) HUMPHOSLIP_PEA_2_T16 825 888 (SEQ ID NO: 677) HUMPHOSLIP_PEA_2_T17 540 603 (SEQ ID NO: 678) HUMPHOSLIP_PEA_2_T18 754 817 (SEQ ID NO: 679) HUMPHOSLIP_PEA_2_T19 825 888 (SEQ ID NO: 680)

Segment cluster HUMPHOSLIP_PEA_(—)2_node_(—)29 (SEQ ID NO: 702) according to the present invention can be found in the following transcript(s): HUMPHOSLIP_PEA_(—)2_T6 (SEQ ID NO: 674), HUMPHOSLIP_PEA_(—)2_T7 (SEQ ID NO: 675), HUMPHOSLIP_PEA_(—)2_T14 (SEQ ID NO: 676), HUMPHOSLIP_PEA_(—)2_T17 (SEQ ID NO: 678), HUMPHOSLIP_PEA_(—)2_T18 (SEQ ID NO: 679) and HUMPHOSLIP_PEA_(—)2_T19 (SEQ ID NO: 680). Table 47 below describes the starting and ending position of this segment on each transcript.

TABLE 47 Segment location on transcripts Segment Segment starting ending Transcript name position position HUMPHOSLIP_PEA_2_T6 843 849 (SEQ ID NO: 674) HUMPHOSLIP_PEA_2_T7 981 987 (SEQ ID NO: 675) HUMPHOSLIP_PEA_2_T14 974 980 (SEQ ID NO: 676) HUMPHOSLIP_PEA_2_T17 604 610 (SEQ ID NO: 678) HUMPHOSLIP_PEA_2_T18 818 824 (SEQ ID NO: 679) HUMPHOSLIP_PEA_2_T19 889 895 (SEQ ID NO: 680)

Segment cluster HUMPHOSLIP_PEA_(—)2_node_(—)30 (SEQ ID NO: 703) according to the present invention is supported by 181 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HUMPHOSLIP_PEA_(—)2_T6 (SEQ ID NO: 674), HUMPHOSLIP_PEA_(—)2_T7 (SEQ ID NO: 675), HUMPHOSLIP_PEA_(—)2_T14 (SEQ ID NO: 676), HUMPHOSLIP_PEA_(—)2_T16 (SEQ ID NO: 677), HUMPHOSLIP_PEA_(—)2_T17 (SEQ ID NO: 678), HUMPHOSLIP_PEA_(—)2_T18 (SEQ ID NO: 679) and HUMPHOSLIP_PEA_(—)2_T19 (SEQ ID NO: 680). Table 48 below describes the starting and ending position of this segment on each transcript.

TABLE 48 Segment location on transcripts Segment Segment starting ending Transcript name position position HUMPHOSLIP_PEA_2_T6 850 934 (SEQ ID NO: 674) HUMPHOSLIP_PEA_2_T7 988 1072 (SEQ ID NO: 675) HUMPHOSLIP_PEA_2_T14 981 1065 (SEQ ID NO: 676) HUMPHOSLIP_PEA_2_T16 889 973 (SEQ ID NO: 677) HUMPHOSLIP_PEA_2_T17 611 695 (SEQ ID NO: 678) HUMPHOSLIP_PEA_2_T18 825 909 (SEQ ID NO: 679) HUMPHOSLIP_PEA_2_T19 896 980 (SEQ ID NO: 680)

Segment cluster HUMPHOSLIP_PEA_(—)2_node_(—)33 (SEQ ID NO: 704) according to the present invention is supported by 173 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HUMPHOSLIP_PEA_(—)2_T6 (SEQ ID NO: 674), HUMPHOSLIP_PEA_(—)2_T7 (SEQ ID NO: 675), HUMPHOSLIP_PEA_(—)2_T14 (SEQ ID NO: 676), HUMPHOSLIP_PEA_(—)2_T16 (SEQ ID NO: 677), HUMPHOSLIP_PEA_(—)2_T17 (SEQ ID NO: 678), HUMPHOSLIP_PEA_(—)2_T18 (SEQ ID NO: 679) and HUMPHOSLIP_PEA_(—)2_T19 (SEQ ID NO: 680). Table 49 below describes the starting and ending position of this segment on each transcript.

TABLE 49 Segment location on transcripts Segment Segment starting ending Transcript name position position HUMPHOSLIP_PEA_2_T6 935 970 (SEQ ID NO: 674) HUMPHOSLIP_PEA_2_T7 1073 1108 (SEQ ID NO: 675) HUMPHOSLIP_PEA_2_T14 1066 1101 (SEQ ID NO: 676) HUMPHOSLIP_PEA_2_T16 974 1009 (SEQ ID NO: 677) HUMPHOSLIP_PEA_2_T17 696 731 (SEQ ID NO: 678) HUMPHOSLIP_PEA_2_T18 910 945 (SEQ ID NO: 679) HUMPHOSLIP_PEA_2_T19 981 1016 (SEQ ID NO: 680)

Segment cluster HUMPHOSLIP_PEA_(—)2_node_(—)36 (SEQ ID NO: 705) according to the present invention is supported by 163 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HUMPHOSLIP_PEA_(—)2_T6 (SEQ ID NO: 674), HUMPHOSLIP_PEA_(—)2_T7 (SEQ ID NO: 675), HUMPHOSLIP_PEA_(—)2_T14 (SEQ ID NO: 676), HUMPHOSLIP_PEA_(—)2_T16 (SEQ ID NO: 677), HUMPHOSLIP_PEA_(—)2_T17 (SEQ ID NO: 678), HUMPHOSLIP_PEA_(—)2_T18 (SEQ ID NO: 679) and HUMPHOSLIP_PEA_(—)2_T19 (SEQ ID NO: 680). Table 50 below describes the starting and ending position of this segment on each transcript.

TABLE 50 Segment location on transcripts Segment Segment starting ending Transcript name position position HUMPHOSLIP_PEA_2_T6 1112 1156 (SEQ ID NO: 674) HUMPHOSLIP_PEA_2_T7 1250 1294 (SEQ ID NO: 675) HUMPHOSLIP_PEA_2_T14 1243 1287 (SEQ ID NO: 676) HUMPHOSLIP_PEA_2_T16 1151 1195 (SEQ ID NO: 677) HUMPHOSLIP_PEA_2_T17 873 917 (SEQ ID NO: 678) HUMPHOSLIP_PEA_2_T18 1087 1131 (SEQ ID NO: 679) HUMPHOSLIP_PEA_2_T19 1158 1202 (SEQ ID NO: 680)

Segment cluster HUMPHOSLIP_PEA_(—)2_node_(—)37 (SEQ ID NO: 706) according to the present invention can be found in the following transcript(s): HUMPHOSLIP_PEA_(—)2_T6 (SEQ ID NO: 674), HUMPHOSLIP_PEA_(—)2_T7 (SEQ ID NO: 675), HUMPHOSLIP_PEA_(—)2_T14 (SEQ ID NO: 676), HUMPHOSLIP_PEA_(—)2_T16 (SEQ ID NO: 677), HUMPHOSLIP_PEA_(—)2_T17 (SEQ ID NO: 678), HUMPHOSLIP_PEA_(—)2_T18 (SEQ ID NO: 679) and HUMPHOSLIP_PEA_(—)2_T19 (SEQ ID NO: 680). Table 51 below describes the starting and ending position of this segment on each transcript.

TABLE 51 Segment location on transcripts Segment Segment starting ending Transcript name position position HUMPHOSLIP_PEA_2_T6 1157 1171 (SEQ ID NO: 674) HUMPHOSLIP_PEA_2_T7 1295 1309 (SEQ ID NO: 675) HUMPHOSLIP_PEA_2_T14 1288 1302 (SEQ ID NO: 676) HUMPHOSLIP_PEA_2_T16 1196 1210 (SEQ ID NO: 677) HUMPHOSLIP_PEA_2_T17 918 932 (SEQ ID NO: 678) HUMPHOSLIP_PEA_2_T18 1132 1146 (SEQ ID NO: 679) HUMPHOSLIP_PEA_2_T19 1203 1217 (SEQ ID NO: 680)

Segment cluster HUMPHOSLIP_PEA_(—)2_node_(—)39 (SEQ ID NO: 707) according to the present invention is supported by 166 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HUMPHOSLIP_PEA_(—)2_T6 (SEQ ID NO: 674), HUMPHOSLIP_PEA_(—)2_T7 (SEQ ID NO: 675), HUMPHOSLIP_PEA_(—)2_T14 (SEQ ID NO: 676), HUMPHOSLIP_PEA_(—)2_T16 (SEQ ID NO: 677), HUMPHOSLIP_PEA_(—)2_T17 (SEQ ID NO: 678), HUMPHOSLIP_PEA_(—)2_T18 (SEQ ID NO: 679) and HUMPHOSLIP_PEA_(—)2_T19 (SEQ ID NO: 680). Table 52 below describes the starting and ending position of this segment on each transcript.

TABLE 52 Segment location on transcripts Segment Segment starting ending Transcript name position position HUMPHOSLIP_PEA_2_T6 1172 1201 (SEQ ID NO: 674) HUMPHOSLIP_PEA_2_T7 1310 1339 (SEQ ID NO: 675) HUMPHOSLIP_PEA_2_T14 1303 1332 (SEQ ID NO: 676) HUMPHOSLIP_PEA_2_T16 1211 1240 (SEQ ID NO: 677) HUMPHOSLIP_PEA_2_T17 933 962 (SEQ ID NO: 678) HUMPHOSLIP_PEA_2_T18 1147 1176 (SEQ ID NO: 679) HUMPHOSLIP_PEA_2_T19 1218 1247 (SEQ ID NO: 680)

Segment cluster HUMPHOSLIP_PEA_(—)2_node_(—)40 (SEQ ID NO: 708) according to the present invention is supported by 199 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HUMPHOSLIP_PEA_(—)2_T6 (SEQ ID NO: 674), HUMPHOSLIP_PEA_(—)2_T7 (SEQ ID NO: 675), HUMPHOSLIP_PEA_(—)2_T14 (SEQ ID NO: 676), HUMPHOSLIP_PEA_(—)2_T16 (SEQ ID NO: 677), HUMPHOSLIP_PEA_(—)2_T17 (SEQ ID NO: 678), HUMPHOSLIP_PEA_(—)2_T18 (SEQ ID NO: 679) and HUMPHOSLIP_PEA_(—)2_T19 (SEQ ID NO: 680). Table 53 below describes the starting and ending position of this segment on each transcript.

TABLE 53 Segment location on transcripts Segment Segment starting ending Transcript name position position HUMPHOSLIP_PEA_2_T6 1202 1288 (SEQ ID NO: 674) HUMPHOSLIP_PEA_2_T7 1340 1426 (SEQ ID NO: 675) HUMPHOSLIP_PEA_2_T14 1333 1419 (SEQ ID NO: 676) HUMPHOSLIP_PEA_2_T16 1241 1327 (SEQ ID NO: 677) HUMPHOSLIP_PEA_2_T17 963 1049 (SEQ ID NO: 678) HUMPHOSLIP_PEA_2_T18 1177 1263 (SEQ ID NO: 679) HUMPHOSLIP_PEA_2_T19 1248 1334 (SEQ ID NO: 680)

Segment cluster HUMPHOSLIP_PEA_(—)2_node_(—)41 (SEQ ID NO: 709) according to the present invention is supported by 186 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HUMPHOSLIP_PEA_(—)2_T6 (SEQ ID NO: 674), HUMPHOSLIP_PEA_(—)2_T7 (SEQ ID NO: 675), HUMPHOSLIP_PEA_(—)2_T14 (SEQ ID NO: 676), HUMPHOSLIP_PEA_(—)2_T16 (SEQ ID NO: 677), HUMPHOSLIP_PEA_(—)2_T17 (SEQ ID NO: 678), HUMPHOSLIP_PEA_(—)2_T18 (SEQ ID NO: 679) and HUMPHOSLIP_PEA_(—)2_T19 (SEQ ID NO: 680). Table 54 below describes the starting and ending position of this segment on each transcript.

TABLE 54 Segment location on transcripts Segment Segment starting ending Transcript name position position HUMPHOSLIP_PEA_2_T6 1289 1318 (SEQ ID NO: 674) HUMPHOSLIP_PEA_2_T7 1427 1456 (SEQ ID NO: 675) HUMPHOSLIP_PEA_2_T14 1420 1449 (SEQ ID NO: 676) HUMPHOSLIP_PEA_2_T16 1328 1357 (SEQ ID NO: 677) HUMPHOSLIP_PEA_2_T17 1050 1079 (SEQ ID NO: 678) HUMPHOSLIP_PEA_2_T18 1264 1293 (SEQ ID NO: 679) HUMPHOSLIP_PEA_2_T19 1335 1364 (SEQ ID NO: 680)

Segment cluster HUMPHOSLIP_PEA_(—)2_node_(—)42 (SEQ ID NO: 710) according to the present invention can be found in the following transcript(s): HUMPHOSLIP_PEA_(—)2_T6 (SEQ ID NO: 674), HUMPHOSLIP_PEA_(—)2_T7 (SEQ ID NO: 675), HUMPHOSLIP_PEA_(—)2_T14 (SEQ ID NO: 676), HUMPHOSLIP_PEA_(—)2_T16 (SEQ ID NO: 677), HUMPHOSLIP_PEA_(—)2_T17 (SEQ ID NO: 678), HUMPHOSLIP_PEA_(—)2_T18 (SEQ ID NO: 679) and HUMPHOSLIP_PEA_(—)2_T19 (SEQ ID NO: 680). Table 55 below describes the starting and ending position of this segment on each transcript.

TABLE 55 Segment location on transcripts Segment Segment starting ending Transcript name position position HUMPHOSLIP_PEA_2_T6 1319 1336 (SEQ ID NO: 674) HUMPHOSLIP_PEA_2_T7 1457 1474 (SEQ ID NO: 675) HUMPHOSLIP_PEA_2_T14 1450 1467 (SEQ ID NO: 676) HUMPHOSLIP_PEA_2_T16 1358 1375 (SEQ ID NO: 677) HUMPHOSLIP_PEA_2_T17 1080 1097 (SEQ ID NO: 678) HUMPHOSLIP_PEA_2_T18 1294 1311 (SEQ ID NO: 679) HUMPHOSLIP_PEA_2_T19 1365 1382 (SEQ ID NO: 680)

Segment cluster HUMPHOSLIP_PEA_(—)2_node_(—)44 (SEQ ID NO: 711) according to the present invention is supported by 185 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HUMPHOSLIP_PEA_(—)2_T6 (SEQ ID NO: 674), HUMPHOSLIP_PEA_(—)2_T7 (SEQ ID NO: 675), HUMPHOSLIP_PEA_(—)2_T14 (SEQ ID NO: 676), HUMPHOSLIP_PEA_(—)2_T16 (SEQ ID NO: 677), HUMPHOSLIP_PEA_(—)2_T17 (SEQ ID NO: 678), HUMPHOSLIP_PEA_(—)2_T18 (SEQ ID NO: 679) and HUMPHOSLIP_PEA_(—)2_T19 (SEQ ID NO: 680). Table 56 below describes the starting and ending position of this segment on each transcript.

TABLE 56 Segment location on transcripts Segment Segment starting ending Transcript name position position HUMPHOSLIP_PEA_2_T6 1337 1363 (SEQ ID NO: 674) HUMPHOSLIP_PEA_2_T7 1475 1501 (SEQ ID NO: 675) HUMPHOSLIP_PEA_2_T14 1468 1494 (SEQ ID NO: 676) HUMPHOSLIP_PEA_2_T16 1376 1402 (SEQ ID NO: 677) HUMPHOSLIP_PEA_2_T17 1098 1124 (SEQ ID NO: 678) HUMPHOSLIP_PEA_2_T18 1312 1338 (SEQ ID NO: 679) HUMPHOSLIP_PEA_2_T19 1383 1409 (SEQ ID NO: 680)

Segment cluster HUMPHOSLIP_PEA_(—)2_node_(—)45 (SEQ ID NO: 712) according to the present invention is supported by 197 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HUMPHOSLIP_PEA_(—)2_T6 (SEQ ID NO: 674), HUMPHOSLIP_PEA_(—)2_T7 (SEQ ID NO: 675), HUMPHOSLIP_PEA_(—)2_T14 (SEQ ID NO: 676), HUMPHOSLIP_PEA_(—)2_T16 (SEQ ID NO: 677), HUMPHOSLIP_PEA_(—)2_T17 (SEQ ID NO: 678), HUMPHOSLIP_PEA_(—)2_T18 (SEQ ID NO: 679) and HUMPHOSLIP_PEA_(—)2_T19 (SEQ ID NO: 680). Table 57 below describes the starting and ending position of this segment on each transcript.

TABLE 57 Segment location on transcripts Segment Segment starting ending Transcript name position position HUMPHOSLIP_PEA_2_T6 1364 1404 (SEQ ID NO: 674) HUMPHOSLIP_PEA_2_T7 1502 1542 (SEQ ID NO: 675) HUMPHOSLIP_PEA_2_T14 1495 1535 (SEQ ID NO: 676) HUMPHOSLIP_PEA_2_T16 1403 1443 (SEQ ID NO: 677) HUMPHOSLIP_PEA_2_T17 1125 1165 (SEQ ID NO: 678) HUMPHOSLIP_PEA_2_T18 1339 1379 (SEQ ID NO: 679) HUMPHOSLIP_PEA_2_T19 1410 1450 (SEQ ID NO: 680)

Segment cluster HUMPHOSLIP_PEA_(—)2_node_(—)47 (SEQ ID NO: 713) according to the present invention is supported by 223 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HUMPHOSLIP_PEA_(—)2_T6 (SEQ ID NO: 674), HUMPHOSLIP_PEA_(—)2_T7 (SEQ ID NO: 675), HUMPHOSLIP_PEA_(—)2_T14 (SEQ ID NO: 676), HUMPHOSLIP_PEA_(—)2_T16 (SEQ ID NO: 677), HUMPHOSLIP_PEA_(—)2_T17 (SEQ ID NO: 678), HUMPHOSLIP_PEA_(—)2_T18 (SEQ ID NO: 679) and HUMPHOSLIP_PEA_(—)2_T19 (SEQ ID NO: 680). Table 58 below describes the starting and ending position of this segment on each transcript.

TABLE 58 Segment location on transcripts Segment Segment starting ending Transcript name position position HUMPHOSLIP_PEA_2_T6 1405 1447 (SEQ ID NO: 674) HUMPHOSLIP_PEA_2_T7 1543 1585 (SEQ ID NO: 675) HUMPHOSLIP_PEA_2_T14 1536 1578 (SEQ ID NO: 676) HUMPHOSLIP_PEA_2_T16 1444 1486 (SEQ ID NO: 677) HUMPHOSLIP_PEA_2_T17 1166 1208 (SEQ ID NO: 678) HUMPHOSLIP_PEA_2_T18 1380 1422 (SEQ ID NO: 679) HUMPHOSLIP_PEA_2_T19 1451 1493 (SEQ ID NO: 680)

Segment cluster HUMPHOSLIP_PEA_(—)2_node_(—)51 (SEQ ID NO: 714) according to the present invention can be found in the following transcript(s): HUMPHOSLIP_PEA_(—)2_T6 (SEQ ID NO: 674), HUMPHOSLIP_PEA_(—)2_T7 (SEQ ID NO: 675), HUMPHOSLIP_PEA_(—)2_T14 (SEQ ID NO: 676), HUMPHOSLIP_PEA_(—)2_T16 (SEQ ID NO: 677), HUMPHOSLIP_PEA_(—)2_T17 (SEQ ID NO: 678), HUMPHOSLIP_PEA_(—)2_T18 (SEQ ID NO: 679) and HUMPHOSLIP_PEA_(—)2_T19 (SEQ ID NO: 680). Table 59 below describes the starting and ending position of this segment on each transcript.

TABLE 59 Segment location on transcripts Segment Segment starting ending Transcript name position position HUMPHOSLIP_PEA_2_T6 1448 1462 (SEQ ID NO: 674) HUMPHOSLIP_PEA_2_T7 1586 1600 (SEQ ID NO: 675) HUMPHOSLIP_PEA_2_T14 1579 1593 (SEQ ID NO: 676) HUMPHOSLIP_PEA_2_T16 1487 1501 (SEQ ID NO: 677) HUMPHOSLIP_PEA_2_T17 1209 1223 (SEQ ID NO: 678) HUMPHOSLIP_PEA_2_T18 1423 1437 (SEQ ID NO: 679) HUMPHOSLIP_PEA_2_T19 1494 1508 (SEQ ID NO: 680)

Segment cluster HUMPHOSLIP_PEA_(—)2_node_(—)52 (SEQ ID NO: 715) according to the present invention is supported by 235 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HUMPHOSLIP_PEA_(—)2_T6 (SEQ ID NO: 674), HUMPHOSLIP_PEA_(—)2_T7 (SEQ ID NO: 675), HUMPHOSLIP_PEA_(—)2_T14 (SEQ ID NO: 676), HUMPHOSLIP_PEA_(—)2_T16 (SEQ ID NO: 677), HUMPHOSLIP_PEA_(—)2_T17 (SEQ ID NO: 678), HUMPHOSLIP_PEA_(—)2_T18 (SEQ ID NO: 679) and HUMPHOSLIP_PEA_(—)2_T19 (SEQ ID NO: 680). Table 60 below describes the starting and ending position of this segment on each transcript.

TABLE 60 Segment location on transcripts Segment Segment Transcript starting ending name position position HUMPHOSLIP_PEA_2_T6 1463 1511 (SEQ ID NO: 674) HUMPHOSLIP_PEA_2_T7 1601 1649 (SEQ ID NO: 675) HUMPHOSLIP_PEA_2_T14 1594 1642 (SEQ ID NO: 676) HUMPHOSLIP_PEA_2_T16 1502 1550 (SEQ ID NO: 677) HUMPHOSLIP_PEA_2_T17 1224 1272 (SEQ ID NO: 678) HUMPHOSLIP_PEA_2_T18 1438 1486 (SEQ ID NO: 679) HUMPHOSLIP_PEA_2_T19 1509 1557 (SEQ ID NO: 680)

Segment cluster HUMPHOSLIP_PEA_(—)2_node_(—)53 (SEQ ID NO: 716) according to the present invention is supported by 5 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HUMPHOSLIP_PEA_(—)2_T19 (SEQ ID NO: 680). Table 61 below describes the starting and ending position of this segment on each transcript.

TABLE 61 Segment location on transcripts Segment Segment Transcript starting ending name position position HUMPHOSLIP_PEA_2_T19 1558 1640 (SEQ ID NO: 680)

Segment cluster HUMPHOSLIP_PEA_(—)2_node_(—)54 (SEQ ID NO: 717) according to the present invention is supported by 236 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HUMPHOSLIP_PEA_(—)2_T6 (SEQ ID NO: 674), HUMPHOSLIP_PEA_(—)2_T7 (SEQ ID NO: 675), HUMPHOSLIP_PEA_(—)2_T14 (SEQ ID NO: 676), HUMPHOSLIP_PEA_(—)2_T16 (SEQ ID NO: 677), HUMPHOSLIP_PEA_(—)2_T17 (SEQ ID NO: 678), HUMPHOSLIP_PEA_(—)2_T18 (SEQ ID NO: 679) and HUMPHOSLIP_PEA_(—)2_T19 (SEQ ID NO: 680). Table 62 below describes the starting and ending position of this segment on each transcript.

TABLE 62 Segment location on transcripts Segment Segment Transcript starting ending name position position HUMPHOSLIP_PEA_2_T6 1512 1552 (SEQ ID NO: 674) HUMPHOSLIP_PEA_2_T7 1650 1690 (SEQ ID NO: 675) HUMPHOSLIP_PEA_2_T14 1643 1683 (SEQ ID NO: 676) HUMPHOSLIP_PEA_2_T16 1551 1591 (SEQ ID NO: 677) HUMPHOSLIP_PEA_2_T17 1273 1313 (SEQ ID NO: 678) HUMPHOSLIP_PEA_2_T18 1487 1527 (SEQ ID NO: 679) HUMPHOSLIP_PEA_2_T19 1641 1681 (SEQ ID NO: 680)

Segment cluster HUMPHOSLIP_PEA_(—)2_node_(—)55 (SEQ ID NO: 718) according to the present invention is supported by 232 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HUMPHOSLIP_PEA_(—)2_T6 (SEQ ID NO: 674), HUMPHOSLIP_PEA_(—)2_T7 (SEQ ID NO: 675), HUMPHOSLIP_PEA_(—)2_T14 (SEQ ID NO: 676), HUMPHOSLIP_PEA_(—)2_T16 (SEQ ID NO: 677), HUMPHOSLIP_PEA_(—)2_T17 (SEQ ID NO: 678), HUMPHOSLIP_PEA_(—)2_T18 (SEQ ID NO: 679) and HUMPHOSLIP_PEA_(—)2_T19 (SEQ ID NO: 680). Table 63 below describes the starting and ending position of this segment on each transcript.

TABLE 63 Segment location on transcripts Segment Segment Transcript starting ending name position position HUMPHOSLIP_PEA_2_T6 1553 1588 (SEQ ID NO: 674) HUMPHOSLIP_PEA_2_T7 1691 1726 (SEQ ID NO: 675) HUMPHOSLIP_PEA_2_T14 1684 1719 (SEQ ID NO: 676) HUMPHOSLIP_PEA_2_T16 1592 1627 (SEQ ID NO: 677) HUMPHOSLIP_PEA_2_T17 1314 1349 (SEQ ID NO: 678) HUMPHOSLIP_PEA_2_T18 1528 1563 (SEQ ID NO: 679) HUMPHOSLIP_PEA_2_T19 1682 1717 (SEQ ID NO: 680)

Segment cluster HUMPHOSLIP_PEA_(—)2_node_(—)58 (SEQ ID NO: 719) according to the present invention can be found in the following transcript(s): HUMPHOSLIP_PEA_(—)2_T6 (SEQ ID NO: 674), HUMPHOSLIP_PEA_(—)2_T7 (SEQ ID NO: 675), HUMPHOSLIP_PEA_(—)2_T14 (SEQ ID NO: 676), HUMPHOSLIP_PEA_(—)2_T16 (SEQ ID NO: 677), HUMPHOSLIP_PEA_(—)2_T17 (SEQ ID NO: 678), HUMPHOSLIP_PEA_(—)2_T18 (SEQ ID NO: 679) and HUMPHOSLIP_PEA_(—)2_T19 (SEQ ID NO: 680). Table 64 below describes the starting and ending position of this segment on each transcript.

TABLE 64 Segment location on transcripts Segment Segment Transcript starting ending name position position HUMPHOSLIP_PEA_2_T6 1589 1612 (SEQ ID NO: 674) HUMPHOSLIP_PEA_2_T7 1727 1750 (SEQ ID NO: 675) HUMPHOSLIP_PEA_2_T14 1720 1743 (SEQ ID NO: 676) HUMPHOSLIP_PEA_2_T16 1628 1651 (SEQ ID NO: 677) HUMPHOSLIP_PEA_2_T17 1350 1373 (SEQ ID NO: 678) HUMPHOSLIP_PEA_2_T18 1564 1587 (SEQ ID NO: 679) HUMPHOSLIP_PEA_2_T19 1718 1741 (SEQ ID NO: 680)

Segment cluster HUMPHOSLIP_PEA_(—)2_node_(—)59 (SEQ ID NO: 720) according to the present invention is supported by 230 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HUMPHOSLIP_PEA_(—)2_T6 (SEQ ID NO: 674), HUMPHOSLIP_PEA_(—)2_T7 (SEQ ID NO: 675), HUMPHOSLIP_PEA_(—)2_T14 (SEQ ID NO: 676), HUMPHOSLIP_PEA_(—)2_T16 (SEQ ID NO: 677), HUMPHOSLIP_PEA_(—)2_T17 (SEQ ID NO: 678), HUMPHOSLIP_PEA_(—)2_T18 (SEQ ID NO: 679) and HUMPHOSLIP_PEA_(—)2_T19 (SEQ ID NO: 680). Table 65 below describes the starting and ending position of this segment on each transcript.

TABLE 65 Segment location on transcripts Segment Segment Transcript starting ending name position position HUMPHOSLIP_PEA_2_T6 1613 1648 (SEQ ID NO: 674) HUMPHOSLIP_PEA_2_T7 1751 1786 (SEQ ID NO: 675) HUMPHOSLIP_PEA_2_T14 1744 1779 (SEQ ID NO: 676) HUMPHOSLIP_PEA_2_T16 1652 1687 (SEQ ID NO: 677) HUMPHOSLIP_PEA_2_T17 1374 1409 (SEQ ID NO: 678) HUMPHOSLIP_PEA_2_T18 1588 1623 (SEQ ID NO: 679) HUMPHOSLIP_PEA_2_T19 1742 1777 (SEQ ID NO: 680)

Segment cluster HUMPHOSLIP_PEA_(—)2_node_(—)60 (SEQ ID NO: 721) according to the present invention can be found in the following transcript(s): HUMPHOSLIP_PEA_(—)2_T6 (SEQ ID NO: 674), HUMPHOSLIP_PEA_(—)2_T7 (SEQ ID NO: 675), HUMPHOSLIP_PEA_(—)2_T14 (SEQ ID NO: 676), HUMPHOSLIP_PEA_(—)2_T16 (SEQ ID NO: 677), HUMPHOSLIP_PEA_(—)2_T17 (SEQ ID NO: 678), HUMPHOSLIP_PEA_(—)2_T18 (SEQ ID NO: 679) and HUMPHOSLIP_PEA_(—)2_T19 (SEQ ID NO: 680). Table 66 below describes the starting and ending position of this segment on each transcript.

TABLE 66 Segment location on transcripts Segment Segment Transcript starting ending name position position HUMPHOSLIP_PEA_2_T6 1649 1671 (SEQ ID NO: 674) HUMPHOSLIP_PEA_2_T7 1787 1809 (SEQ ID NO: 675) HUMPHOSLIP_PEA_2_T14 1780 1802 (SEQ ID NO: 676) HUMPHOSLIP_PEA_2_T16 1688 1710 (SEQ ID NO: 677) HUMPHOSLIP_PEA_2_T17 1410 1432 (SEQ ID NO: 678) HUMPHOSLIP_PEA_2_T18 1624 1646 (SEQ ID NO: 679) HUMPHOSLIP_PEA_2_T19 1778 1800 (SEQ ID NO: 680)

Segment cluster HUMPHOSLIP_PEA_(—)2_node_(—)61 (SEQ ID NO: 722) according to the present invention can be found in the following transcript(s): HUMPHOSLIP_PEA_(—)2_T6 (SEQ ID NO: 674), HUMPHOSLIP_PEA_(—)2_T7 (SEQ ID NO: 675), HUMPHOSLIP_PEA_(—)2_T14 (SEQ ID NO: 676), HUMPHOSLIP_PEA_(—)2_T16 (SEQ ID NO: 677), HUMPHOSLIP_PEA_(—)2_T17 (SEQ ID NO: 678), HUMPHOSLIP_PEA_(—)2_T18 (SEQ ID NO: 679) and HUMPHOSLIP_PEA_(—)2_T19 (SEQ ID NO: 680). Table 67 below describes the starting and ending position of this segment on each transcript.

TABLE 67 Segment location on transcripts Segment Segment Transcript starting ending name position position HUMPHOSLIP_PEA_2_T6 1672 1680 (SEQ ID NO: 674) HUMPHOSLIP_PEA_2_T7 1810 1818 (SEQ ID NO: 675) HUMPHOSLIP_PEA_2_T14 1803 1811 (SEQ ID NO: 676) HUMPHOSLIP_PEA_2_T16 1711 1719 (SEQ ID NO: 677) HUMPHOSLIP_PEA_2_T17 1433 1441 (SEQ ID NO: 678) HUMPHOSLIP_PEA_2_T18 1647 1655 (SEQ ID NO: 679) HUMPHOSLIP_PEA_2_T19 1801 1809 (SEQ ID NO: 680)

Segment cluster HUMPHOSLIP_PEA_(—)2_node_(—)62 (SEQ ID NO: 723) according to the present invention can be found in the following transcript(s): HUMPHOSLIP_PEA_(—)2_T6 (SEQ ID NO: 674), HUMPHOSLIP_PEA_(—)2_T7 (SEQ ID NO: 675), HUMPHOSLIP_PEA_(—)2_T14 (SEQ ID NO: 676), HUMPHOSLIP_PEA_(—)2_T16 (SEQ ID NO: 677), HUMPHOSLIP_PEA_(—)2_T17 (SEQ ID NO: 678), HUMPHOSLIP_PEA_(—)2_T18 (SEQ ID NO: 679) and HUMPHOSLIP_PEA_(—)2_T19 (SEQ ID NO: 680). Table 68 below describes the starting and ending position of this segment on each transcript.

TABLE 68 Segment location on transcripts Segment Segment Transcript starting ending name position position HUMPHOSLIP_PEA_2_T6 1681 1703 (SEQ ID NO: 674) HUMPHOSLIP_PEA_2_T7 1819 1841 (SEQ ID NO: 675) HUMPHOSLIP_PEA_2_T14 1812 1834 (SEQ ID NO: 676) HUMPHOSLIP_PEA_2_T16 1720 1742 (SEQ ID NO: 677) HUMPHOSLIP_PEA_2_T17 1442 1464 (SEQ ID NO: 678) HUMPHOSLIP_PEA_2_T18 1656 1678 (SEQ ID NO: 679) HUMPHOSLIP_PEA_2_T19 1810 1832 (SEQ ID NO: 680)

Segment cluster HUMPHOSLIP_PEA_(—)2_node_(—)63 (SEQ ID NO: 724) according to the present invention can be found in the following transcript(s): HUMPHOSLIP_PEA_(—)2_T6 (SEQ ID NO: 674), HUMPHOSLIP_PEA_(—)2_T7 (SEQ ID NO: 675), HUMPHOSLIP_PEA_(—)2_T14 (SEQ ID NO: 676), HUMPHOSLIP_PEA_(—)2_T16 (SEQ ID NO: 677), HUMPHOSLIP_PEA_(—)2_T17 (SEQ ID NO: 678), HUMPHOSLIP_PEA_(—)2_T18 (SEQ ID NO: 679) and HUMPHOSLIP_PEA_(—)2_T19 (SEQ ID NO: 680). Table 69 below describes the starting and ending position of this segment on each transcript.

TABLE 69 Segment location on transcripts Segment Segment Transcript starting ending name position position HUMPHOSLIP_PEA_2_T6 1704 1727 (SEQ ID NO: 674) HUMPHOSLIP_PEA_2_T7 1842 1865 (SEQ ID NO: 675) HUMPHOSLIP_PEA_2_T14 1835 1858 (SEQ ID NO: 676) HUMPHOSLIP_PEA_2_T16 1743 1766 (SEQ ID NO: 677) HUMPHOSLIP_PEA_2_T17 1465 1488 (SEQ ID NO: 678) HUMPHOSLIP_PEA_2_T18 1679 1702 (SEQ ID NO: 679) HUMPHOSLIP_PEA_2_T19 1833 1856 (SEQ ID NO: 680)

Segment cluster HUMPHOSLIP_PEA_(—)2_node_(—)64 (SEQ ID NO: 725) according to the present invention can be found in the following transcript(s): HUMPHOSLIP_PEA_(—)2_T6 (SEQ ID NO: 674), HUMPHOSLIP_PEA_(—)2_T7 (SEQ ID NO: 675), HUMPHOSLIP_PEA_(—)2_T14 (SEQ ID NO: 676), HUMPHOSLIP_PEA_(—)2_T16 (SEQ ID NO: 677), HUMPHOSLIP_PEA_(—)2_T17 (SEQ ID NO: 678), HUMPHOSLIP_PEA_(—)2_T18 (SEQ ID NO: 679) and HUMPHOSLIP_PEA_(—)2_T19 (SEQ ID NO: 680). Table 70 below describes the starting and ending position of this segment on each transcript.

TABLE 70 Segment location on transcripts Segment Segment Transcript starting ending name position position HUMPHOSLIP_PEA_2_T6 1728 1734 (SEQ ID NO: 674) HUMPHOSLIP_PEA_2_T7 1866 1872 (SEQ ID NO: 675) HUMPHOSLIP_PEA_2_T14 1859 1865 (SEQ ID NO: 676) HUMPHOSLIP_PEA_2_T16 1767 1773 (SEQ ID NO: 677) HUMPHOSLIP_PEA_2_T17 1489 1495 (SEQ ID NO: 678) HUMPHOSLIP_PEA_2_T18 1703 1709 (SEQ ID NO: 679) HUMPHOSLIP_PEA_2_T19 1857 1863 (SEQ ID NO: 680)

Segment cluster HUMPHOSLIP_PEA_(—)2_node_(—)65 (SEQ ID NO: 726) according to the present invention can be found in the following transcript(s): HUMPHOSLIP_PEA_(—)2_T6 (SEQ ID NO: 674), HUMPHOSLIP_PEA_(—)2_T7 (SEQ ID NO: 675), HUMPHOSLIP_PEA_(—)2_T14 (SEQ ID NO: 676), HUMPHOSLIP_PEA_(—)2_T16 (SEQ ID NO: 677), HUMPHOSLIP_PEA_(—)2_T17 (SEQ ID NO: 678), HUMPHOSLIP_PEA_(—)2_T18 (SEQ ID NO: 679) and HUMPHOSLIP_PEA_(—)2_T19 (SEQ ID NO: 680). Table 71 below describes the starting and ending position of this segment on each transcript.

TABLE 71 Segment location on transcripts Segment Segment Transcript starting ending name position position HUMPHOSLIP_PEA_2_T6 1735 1754 (SEQ ID NO: 674) HUMPHOSLIP_PEA_2_T7 1873 1892 (SEQ ID NO: 675) HUMPHOSLIP_PEA_2_T14 1866 1885 (SEQ ID NO: 676) HUMPHOSLIP_PEA_2_T16 1774 1793 (SEQ ID NO: 677) HUMPHOSLIP_PEA_2_T17 1496 1515 (SEQ ID NO: 678) HUMPHOSLIP_PEA_2_T18 1710 1729 (SEQ ID NO: 679) HUMPHOSLIP_PEA_2_T19 1864 1883 (SEQ ID NO: 680)

Segment cluster HUMPHOSLIP_PEA_(—)2_node_(—)66 (SEQ ID NO: 727) according to the present invention is supported by 180 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HUMPHOSLIP_PEA_(—)2_T6 (SEQ ID NO: 674), HUMPHOSLIP_PEA_(—±2)_T7 (SEQ ID NO: 675), HUMPHOSLIP_PEA_(—)2_T14 (SEQ ID NO: 676), HUMPHOSLIP_PEA_(—)2_T16 (SEQ ID NO: 677), HUMPHOSLIP_PEA_(—)2_T17 (SEQ ID NO: 678), HUMPHOSLIP_PEA_(—)2_T18 (SEQ ID NO: 679) and HUMPHOSLIP_PEA_(—)2_T19 (SEQ ID NO: 680). Table 72 below describes the starting and ending position of this segment on each transcript.

TABLE 72 Segment location on transcripts Segment Segment Transcript starting ending name position position HUMPHOSLIP_PEA_2_T6 1755 1844 (SEQ ID NO: 674) HUMPHOSLIP_PEA_2_T7 1893 1982 (SEQ ID NO: 675) HUMPHOSLIP_PEA_2_T14 1886 1975 (SEQ ID NO: 676) HUMPHOSLIP_PEA_2_T16 1794 1883 (SEQ ID NO: 677) HUMPHOSLIP_PEA_2_T17 1516 1605 (SEQ ID NO: 678) HUMPHOSLIP_PEA_2_T18 1730 1819 (SEQ ID NO: 679) HUMPHOSLIP_PEA_2_T19 1884 1973 (SEQ ID NO: 680)

Segment cluster HUMPHOSLIP_PEA_(—)2_node_(—)67 (SEQ ID NO: 728) according to the present invention can be found in the following transcript(s): HUMPHOSLIP_PEA_(—)2_T6 (SEQ ID NO: 674), HUMPHOSLIP_PEA_(—)2_T7 (SEQ ID NO: 675), HUMPHOSLIP_PEA_(—)2_T14 (SEQ ID NO: 676), HUMPHOSLIP_PEA_(—)2_T16 (SEQ ID NO: 677), HUMPHOSLIP_PEA_(—)2_T17 (SEQ ID NO: 678), HUMPHOSLIP_PEA_(—)2_T18 (SEQ ID NO: 679) and HUMPHOSLIP_PEA_(—)2_T19 (SEQ ID NO: 680). Table 73 below describes the starting and ending position of this segment on each transcript.

TABLE 73 Segment location on transcripts Segment Segment Transcript starting ending name position position HUMPHOSLIP_PEA_2_T6 1845 1866 (SEQ ID NO: 674) HUMPHOSLIP_PEA_2_T7 1983 2004 (SEQ ID NO: 675) HUMPHOSLIP_PEA_2_T14 1976 1997 (SEQ ID NO: 676) HUMPHOSLIP_PEA_2_T16 1884 1905 (SEQ ID NO: 677) HUMPHOSLIP_PEA_2_T17 1606 1627 (SEQ ID NO: 678) HUMPHOSLIP_PEA_2_T18 1820 1841 (SEQ ID NO: 679) HUMPHOSLIP_PEA_2_T19 1974 1995 (SEQ ID NO: 680)

Segment cluster HUMPHOSLIP_PEA_(—)2_node_(—)69 (SEQ ID NO: 729) according to the present invention can be found in the following transcript(s): HUMPHOSLIP_PEA_(—)2_T6 (SEQ ID NO: 674), HUMPHOSLIP_PEA_(—)2_T7 (SEQ ID NO: 675), HUMPHOSLIP_PEA_(—)2_T14 (SEQ ID NO: 676), HUMPHOSLIP_PEA_(—)2_T16 (SEQ ID NO: 677), HUMPHOSLIP_PEA_(—)2_T17 (SEQ ID NO: 678), HUMPHOSLIP_PEA_(—)2_T18 (SEQ ID NO: 679) and HUMPHOSLIP_PEA_(—)2_T19 (SEQ ID NO: 680). Table 74 below describes the starting and ending position of this segment on each transcript.

TABLE 74 Segment location on transcripts Segment Segment Transcript starting ending name position position HUMPHOSLIP_PEA_2_T6 2286 2297 (SEQ ID NO: 674) HUMPHOSLIP_PEA_2_T7 2424 2435 (SEQ ID NO: 675) HUMPHOSLIP_PEA_2_T14 2417 2428 (SEQ ID NO: 676) HUMPHOSLIP_PEA_2_T16 2325 2336 (SEQ ID NO: 677) HUMPHOSLIP_PEA_2_T17 2047 2058 (SEQ ID NO: 678) HUMPHOSLIP_PEA_2_T18 2261 2272 (SEQ ID NO: 679) HUMPHOSLIP_PEA_2_T19 2415 2426 (SEQ ID NO: 680)

Segment cluster HUMPHOSLIP_PEA_(—)2_node_(—)71 (SEQ ID NO: 730) according to the present invention can be found in the following transcript(s): HUMPHOSLIP_PEA_(—)2_T6 (SEQ ID NO: 674), HUMPHOSLIP_PEA_(—)2_T7 (SEQ ID NO: 675), HUMPHOSLIP_PEA_(—)2_T14 (SEQ ID NO: 676), HUMPHOSLIP_PEA_(—)2_T16 (SEQ ID NO: 677), HUMPHOSLIP_PEA_(—)2_T17 (SEQ ID NO: 678), HUMPHOSLIP_PEA_(—)2_T18 (SEQ ID NO: 679) and HUMPHOSLIP_PEA_(—)2_T19 (SEQ ID NO: 680). Table 75 below describes the starting and ending position of this segment on each transcript.

TABLE 75 Segment location on transcripts Segment Segment Transcript starting ending name position position HUMPHOSLIP_PEA_2_T6 2530 2542 (SEQ ID NO: 674) HUMPHOSLIP_PEA_2_T7 2668 2680 (SEQ ID NO: 675) HUMPHOSLIP_PEA_2_T14 2661 2673 (SEQ ID NO: 676) HUMPHOSLIP_PEA_2_T16 2569 2581 (SEQ ID NO: 677) HUMPHOSLIP_PEA_2_T17 2291 2303 (SEQ ID NO: 678) HUMPHOSLIP_PEA_2_T18 2505 2517 (SEQ ID NO: 679) HUMPHOSLIP_PEA_2_T19 2659 2671 (SEQ ID NO: 680)

Segment cluster HUMPHOSLIP_PEA_(—)2_node_(—)72 (SEQ ID NO. 731) according to the present invention is supported by 7 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HUMPHOSLIP_PEA_(—)2_T6 (SEQ ID NO: 674), HUMPHOSLIP_PEA_(—)2_T7 (SEQ ID NO: 675), HUMPHOSLIP_PEA_(—)2_T14 (SEQ ID NO: 676), HUMPHOSLIP_PEA_(—)2_T16 (SEQ ID NO: 677), HUMPHOSLIP_PEA_(—)2_T17 (SEQ ID NO: 678), HUMPHOSLIP_PEA_(—)2_T18 (SEQ ID NO: 679) and HUMPHOSLIP_PEA_(—)2_T19 (SEQ ID NO: 680). Table 76 below describes the starting and ending position of this segment on each transcript.

TABLE 76 Segment location on transcripts Segment Segment Transcript starting ending name position position HUMPHOSLIP_PEA_2_T6 2543 2647 (SEQ ID NO: 674) HUMPHOSLIP_PEA_2_T7 2681 2785 (SEQ ID NO: 675) HUMPHOSLIP_PEA_2_T14 2674 2778 (SEQ ID NO: 676) HUMPHOSLIP_PEA_2_T16 2582 2686 (SEQ ID NO: 677) HUMPHOSLIP_PEA_2_T17 2304 2408 (SEQ ID NO: 678) HUMPHOSLIP_PEA_2_T18 2518 2622 (SEQ ID NO: 679) HUMPHOSLIP_PEA_2_T19 2672 2776 (SEQ ID NO: 680)

Segment cluster HUMPHOSLIP_PEA_(—)2_node_(—)73 (SEQ ID NO: 732) according to the present invention is supported by 5 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HUMPHOSLIP_PEA_(—)2_T6 (SEQ ID NO: 674), HUMPHOSLIP_PEA_(—)2_T7 (SEQ ID NO: 675), HUMPHOSLIP_PEA_(—)2_T14 (SEQ ID NO: 676), HUMPHOSLIP_PEA_(—)2_T16 (SEQ ID NO: 677), HUMPHOSLIP_PEA_(—)2_T17 (SEQ ID NO: 678), HUMPHOSLIP_PEA_(—)2_T18 (SEQ ID NO: 679) and HUMPHOSLIP_PEA_(—)2_T19 (SEQ ID NO: 680). Table 77 below describes the starting and ending position of this segment on each transcript.

TABLE 77 Segment location on transcripts Segment Segment Transcript starting ending name position position HUMPHOSLIP_PEA_2_T6 2648 2755 (SEQ ID NO: 674) HUMPHOSLIP_PEA_2_T7 2786 2893 (SEQ ID NO: 675) HUMPHOSLIP_PEA_2_T14 2779 2886 (SEQ ID NO: 676) HUMPHOSLIP_PEA_2_T16 2687 2794 (SEQ ID NO: 677) HUMPHOSLIP_PEA_2_T17 2409 2516 (SEQ ID NO: 678) HUMPHOSLIP_PEA_2_T18 2623 2730 (SEQ ID NO: 679) HUMPHOSLIP_PEA_2_T19 2777 2884 (SEQ ID NO: 680)

Segment cluster HUMPHOSLIP_PEA_(—)2_node_(—)74 (SEQ ID NO: 733) according to the present invention is supported by 10 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HUMPHOSLIP_PEA_(—)2_T6 (SEQ ID NO: 674), HUMPHOSLIP_PEA_(—)2_T7 (SEQ ID NO: 675), HUMPHOSLIP_PEA_(—)2_T14 (SEQ ID NO: 676), HUMPHOSLIP_PEA_(—)2_T16 (SEQ ID NO: 677), HUMPHOSLIP_PEA_(—)2_T17 (SEQ ID NO: 678), HUMPHOSLIP_PEA_(—)2_T18 (SEQ ID NO: 679) and HUMPHOSLIP_PEA_(—)2_T19 (SEQ ID NO: 680). Table 78 below describes the starting and ending position of this segment on each transcript.

TABLE 76 Segment location on transcripts Segment Segment Transcript starting ending name position position HUMPHOSLIP_PEA_2_T6 2543 2647 (SEQ ID NO: 674) HUMPHOSLIP_PEA_2_T7 2681 2785 (SEQ ID NO: 675) HUMPHOSLIP_PEA_2_T14 2674 2778 (SEQ ID NO: 676) HUMPHOSLIP_PEA_2_T16 2582 2686 (SEQ ID NO: 677) HUMPHOSLIP_PEA_2_T17 2304 2408 (SEQ ID NO: 678) HUMPHOSLIP_PEA_2_T18 2518 2622 (SEQ ID NO: 679) HUMPHOSLIP_PEA_2_T19 2672 2776 (SEQ ID NO: 680) Variant protein alignment to the previously known protein: Sequence name: PLTP_HUMAN Sequence documentation:

Alignment of: HUMPHOSLIP_PEA_(—)2_P10 (SEQ ID NO: 735) x PLTP_HUMAN ..

Alignment segment 1/1:

Quality: 3716.00 Escore: 0 Matching length: 398 Total length: 493 Matching Percent Similarity: 100.00 Matching Percent Identity: 100.00 Total Percent Similarity: 80.73 Total Percent Identity: 80.73 Gaps: 1

Alignment:

Sequence name: PLTP_HUMAN Sequence documentation:

Alignment of: HUMPHOSLIP_PEA_(—)2_P12 (SEQ ID NO: 736) x PLTP_HUMAN ..

Alignment segment 1/1:

Quality: 4101.00 Escore: 0 Matching length: 427 Total length: 427 Matching Percent Similarity: 100.00 Matching Percent Identity: 100.00 Total Percent Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0

Alignment:

Sequence name: PLTP_HUMAN Sequence documentation:

Alignment of: HUMPHOSLIP_PEA_(—)2_P31 (SEQ ID NO: 738) x PLTP_HUMAN ..

Alignment segment 1/1:

Quality: 639.00 Escore: 0 Matching length: 67 Total length: 67 Matching Percent Similarity: 100.00 Matching Percent Identity: 100.00 Total Percent Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0

Alignment:

Sequence name: PLTP_HUMAN Sequence documentation:

Alignment of: HUMPHOSLIP_PEA_(—)2_P33 (SEQ ID NO: 739) x PLTP_HUMAN ..

Alignment segment 1/1:

Quality: 1767.00 Escore: 0 Matching length: 184 Total length: 184 Matching Percent Similarity: 100.00 Matching Percent Identity: 99.46 Total Percent Similarity: 100.00 Total Percent Identity: 99.46 Gaps: 0

Alignment:

Sequence name: PLTP_HUMAN Sequence documentation:

Alignment of: HUMPHOSLIP_PEA_(—)2_P34 (SEQ ID NO: 740) x PLTP_HUMAN ..

Alignment segment 1/1:

Quality: 1971.00 Escore: 0 Matching length: 205 Total length: 205 Matching Percent Similarity: 100.00 Matching Percent Identity: 100.00 Total Percent Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0

Alignment:

Sequence name: PLTP_HUMAN Sequence documentation:

Alignment of: HUMPHOSLIP_PEA_(—)2_P35 (SEQ ID NO: 741) x PLTP_HUMAN ..

Alignment segment 1/1:

Quality: 1158.00 Escore: 0 Matching length: 132 Total length: 184 Matching Percent Similarity: 100.00 Matching Percent Identity: 98.48 Total Percent Similarity: 71.74 Total Percent Identity: 70.65 Gaps: 1

Alignment:

Description for Cluster T59832

Cluster T59832 features 5 transcript(s) and 30 segment(s) of interest, the names for which are given in Tables 1 and 2, respectively, the sequences themselves are given at the end of the application. The selected protein variants are given in table 3.

TABLE 1 Transcripts of interest Transcript Name SEQ ID NO: T59832_T6 742 T59832_T8 743 T59832_T11 744 T59832_T15 745 T59832_T22 746

TABLE 2 Segments of interest Segment Name SEQ ID NO: T59832_node_1 747 T59832_node_7 748 T59832_node_29 749 T59832_node_39 750 T59832_node_2 751 T59832_node_3 752 T59832_node_4 753 T59832_node_5 754 T59832_node_6 755 T59832_node_8 756 T59832_node_9 757 T59832_node_10 758 T59832_node_11 759 T59832_node_12 760 T59832_node_14 761 T59832_node_16 762 T59832_node_19 763 T59832_node_20 764 T59832_node_25 765 T59832_node_26 766 T59832_node_27 767 T59832_node_28 768 T59832_node_30 769 T59832_node_31 770 T59832_node_32 771 T59832_node_34 772 T59832_node_35 773 T59832_node_36 774 T59832_node_37 775 T59832_node_38 776

TABLE 3 Proteins of interest SEQ ID Corresponding Protein Name NO: Transcript(s) T59832_P5 778 T59832_T6 (SEQ ID NO:742) T59832_P7 779 T59832_T8 (SEQ ID NO:743) T59832_P9 780 T59832_T11 (SEQ ID NO:744) T59832_P12 781 T59832_T15 (SEQ ID NO:745) T59832_P18 782 T59832_T22 (SEQ ID NO:746)

These sequences are variants of the known protein Gamma-interferon inducible lysosomal thiol reductase precursor (SwissProt accession identifier GILT_HUMAN; known also according to the synonyms Gamma-interferon-inducible protein IP-30), SEQ ID NO: 777, referred to herein as the previously known protein.

Protein Gamma-interferon inducible lysosomal thiol reductase precursor is known or believed to have the following function(s): cleaves disulfide bonds in proteins by reduction. May facilitate the complete unfolding of proteins destined for lysosomal degradation. May be involved in MHC class II-restricted antigen processing. The sequence for protein Gamma-interferon inducible lysosomal thiol reductase precursor is given at the end of the application, as “Gamma-interferon inducible lysosomal thiol reductase precursor amino acid sequence”. Known polymorphisms for this sequence are as shown in Table 4.

TABLE 4 Amino acid mutations for Known Protein SNP position(s) on amino acid sequence Comment 109 L -> S 130 H -> L 157-261 IVCMEEFEDMERSLPLCLQLYAPGLSPDTIMECAMG DRGMQ LMHANAQRTDALQPPHEYVPWVTVNGKPLEDQTQL LTLVCQ LYQGKKPDVCPSSTSSLRSVCFK -> MSGMAWKSLRTWRE VCHYACSSTPQGCRQNYHGVCNGGPRHAAHARQRP ADRCSP ATARVCALGHRQWETLGRSDPAPYPCLPVVPGQEA GCLPFL NQLPPECLLRVLAGGLRRAHGRRVGTRLPAFFSDPD PRHLL LTNWKILCIP

Protein Gamma-interferon inducible lysosomal thiol reductase precursor localization is believed to be Lysosomal.

The following GO Annotation(s) apply to the previously known protein. The following annotation(s) were found: extracellular; lysosome, which are annotation(s) related to Cellular Component.

The GO assignment relies on information from one or more of the SwissProt/TremBl Protein knowledgebase, available from <http://www.expasy.ch/sprot/>; or Locuslink, available from <http://www.ncbi.nlm.nih.gov/projects/LocusLink/>.

Cluster T59832 can be used as a diagnostic marker according to overexpression of transcripts of this cluster in cancer. Expression of such transcripts in normal tissues is also given according to the previously described methods. The term “number” in the left hand column of the table and the numbers on the y-axis of FIG. 35 refer to weighted expression of ESTs in each category, as “parts per million” (ratio of the expression of ESTs for a particular cluster to the expression of all ESTs in that category, according to parts per million).

Overall, the following results were obtained as shown with regard to the histograms in FIG. 35 and Table 5. This cluster is overexpressed (at least at a minimum level) in the following pathological conditions: brain malignant tumors, breast malignant tumors, ovarian carcinoma and pancreas carcinoma.

TABLE 5 Normal tissue distribution Name of Tissue Number adrenal 208 bladder 205 bone 200 brain 18 colon 236 epithelial 143 general 280 head and neck 192 kidney 71 liver 53 lung 459 lymph nodes 248 breast 0 bone marrow 94 ovary 0 pancreas 20 prostate 86 skin 29 stomach 109 T cells 557 Thyroid 0 uterus 63

TABLE 6 P values and ratios for expression in cancerous tissue Name of Tissue P1 P2 SP1 R3 SP2 R4 adrenal 4.9e−01 5.9e−01 4.7e−03 1.1 2.9e−02 0.8 bladder 3.7e−01 5.6e−01 3.7e−02 1.3 2.5e−01 0.9 bone 6.6e−01 6.7e−01 3.4e−01 0.6 9.1e−01 0.4 brain 1.8e−01 2.9e−01 4.3e−03 3.8 2.8e−02 2.5 colon 4.4e−01 5.2e−01 6.1e−01 0.9 8.1e−01 0.7 epithelial 2.5e−02 1.6e−01 1.2e−05 1.6 9.8e−02 1.1 general 1.3e−02 1.6e−01 1 0.8 1 0.6 head and neck 3.4e−01 3.3e−01 1 0.4 9.4e−01 0.5 kidney 7.7e−01 8.5e−01 1.4e−01 1.3 4.2e−01 0.9 liver 8.3e−01 7.6e−01 1 0.5 1 0.6 lung 5.7e−01 8.3e−01 3.5e−01 0.8 9.8e−01 0.5 lymph nodes 5.7e−01 6.6e−01 7.6e−01 0.8 3.6e−02 1.1 breast 5.0e−02 1.3e−01 2.5e−03 6.5 4.4e−02 3.6 bone marrow 6.2e−01 7.8e−01 1 0.3 9.5e−01 0.5 ovary 2.2e−01 9.4e−02 3.2e−03 6.1 8.3e−03 5.3 pancreas 9.0e−02 1.6e−02 1.1e−03 4.0 7.9e−04 4.2 prostate 8.1e−01 8.0e−01 5.7e−01 0.9 4.1e−01 0.9 skin 1.6e−01 1.2e−01 2.3e−02 6.0 1.0e−02 2.2 stomach 5.5e−01 7.4e−01 9.4e−01 0.6 4.9e−01 1.0 T cells 1 6.7e−01 6.9e−01 1.0 9.8e−01 0.5 Thyroid 2.3e−01 2.3e−01 5.9e−02 2.5 5.9e−02 2.5 uterus 7.4e−02 4.7e−02 2.2e−02 2.0 6.2e−02 1.7

As noted above, cluster T59832 features 5 transcript(s), which were listed in Table 1 above. These transcript(s) encode for protein(s) which are variant(s) of protein Gamma-interferon inducible lysosomal thiol reductase precursor. A description of each variant protein according to the present invention is now provided.

Variant protein T59832_P5 (SEQ ID NO: 778) according to the present invention has an amino acid sequence as given at the end of the application; it is encoded by transcript(s) T59832_T6 (SEQ ID NO: 742). The location of the variant protein was determined according to results from a number of different software programs and analyses, including analyses from SignalP and other specialized programs. The variant protein is believed to be located as follows with regard to the cell: secreted. The protein localization is believed to be secreted because both signal-peptide prediction programs predict that this protein has a signal peptide, and neither trans-membrane region prediction program predicts that this protein has a trans-membrane region.

Variant protein T59832_P5 (SEQ ID NO: 778) is encoded by the following transcript(s): T59832_T6 (SEQ ID NO: 742), for which the sequence(s) is/are given at the end of the application. The coding portion of transcript T59832_T6 (SEQ ID NO: 742) is shown in bold; this coding portion starts at position 149 and ends at position 715. The transcript also has the following SNPs as listed in Table 7 (given according to their position on the nucleotide sequence, with the alternative nucleic acid listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein T59832_P5 (SEQ ID NO: 778) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 7 Nucleic acid SNPs SNP position on nucleotide sequence Alternative nucleic acid Previously known SNP? 61 C -> T Yes 148 G -> T Yes 212 -> A No 241 G -> T No 244 A -> G Yes 962 C -> T Yes 1074 G -> A Yes 1248 G -> C Yes 1441 G -> A Yes 1443 G -> A No 1505 G -> C Yes 1651 T -> No 1652 T -> G Yes 1717 C -> A No 1722 C -> No 1722 C -> G No 1752 A -> G Yes 1817 A -> G Yes 1854 C -> No 1854 C -> A No 1871 C -> T Yes 1886 T -> G No 1906 G -> A No 1906 G -> C No 1942 C -> No 1942 C -> T No 1971 C -> No 1986 G -> A No 2001 G -> T Yes 2008 A -> No 2030 -> T No 2031 C -> T No 2050 C -> No 2056 A -> G Yes 2068 G -> A Yes 2111 A -> C Yes 2136 A -> C Yes 2144 T -> C Yes

Variant protein T59832_P7 (SEQ ID NO: 779) according to the present invention has an amino acid sequence as given at the end of the application; it is encoded by transcript(s) T59832_T8 (SEQ ID NO: 743). An alignment is given to the known protein (Gamma-interferon inducible lysosomal thiol reductase precursor) at the end of the application. One or more alignments to one or more previously published protein sequences are given at the end of the application. A brief description of the relationship of the variant protein according to the present invention to each such aligned protein is as follows:

Comparison report between T59832_P7 (SEQ ID NO: 779) and GILT_HUMAN:

1. An isolated chimeric polypeptide encoding for T59832_P7 (SEQ ID NO: 779), comprising a first amino acid sequence being at least 90% homologous to MTLSPLLLFLPPLLLLLDVPTAAVQASPLQALDFFGNGPPVNYKTGNLYLRGPLKKSNA PLVNVTLYYEALCGGCRAFLIRELFPTWLLVMEILNVTLVPYGNAQEQNVSGRWEFKC QHGEEECKFNKVEACVLDELDMELAFLTIVCMEEFEDMERSLPLCLQLYAPGLSPDTIM ECAMGDRGMQLMHANAQRTDALQPPHEYVPWVTVNG corresponding to amino acids 12-223 of GILT_HUMAN, which also corresponds to amino acids 1-212 of T59832_P7 (SEQ ID NO: 779), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence VRIFLALSLTLIVPWSQGWTRQRDQR (SEQ ID NO: 1089) corresponding to amino acids 213-238 of T59832_P7 (SEQ ID NO: 779), wherein said first amino acid sequence and second amino acid sequence are contiguous and in a sequential order.

2. An isolated polypeptide encoding for a tail of T59832_P7 (SEQ ID NO: 779), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence VRIFLALSLTLIVPWSQGWTRQRDQR (SEQ ID NO: 1089) in T59832_P7 (SEQ ID NO: 779).

The location of the variant protein was determined according to results from a number of different software programs and analyses, including analyses from SignalP and other specialized programs. The variant protein is believed to be located as follows with regard to the cell: secreted. The protein localization is believed to be secreted because both signal-peptide prediction programs predict that this protein has a signal peptide.

Variant protein T59832_P7 (SEQ ID NO: 779) also has the following non-silent SNPs (Single Nucleotide Polymorphisms) as listed in Table 8, (given according to their position(s) on the amino acid sequence, with the alternative amino acid(s) listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein T59832_P7 (SEQ ID NO: 779) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 8 Amino acid mutations SNP position(s) on amino acid sequence Alternative amino acid(s) Previously known SNP? 76 R -> Q Yes 77 A -> T No 146 I -> No 146 I -> M Yes 168 P -> Q No 170 L -> No 170 L -> V No 180 M -> V Yes

The glycosylation sites of variant protein T59832_P7 (SEQ ID NO: 779), as compared to the known protein Gamma-interferon inducible lysosomal thiol reductase precursor, are described in Table 9 (given according to their position(s) on the amino acid sequence in the first column; the second column indicates whether the glycosylation site is present in the variant protein; and the last column indicates whether the position is different on the variant protein).

TABLE 9 Glycosylation site(s) Position(s) on known amino Present in Position in acid sequence variant protein? variant protein? 119 yes 108 106 yes 95 74 yes 63

Variant protein T59832_P7 (SEQ ID NO: 779) is encoded by the following transcript(s): T59832_T8 (SEQ ID NO: 743), for which the sequence(s) is/are given at the end of the application. The coding portion of transcript T59832_T8 (SEQ ID NO: 743) is shown in bold; this coding portion starts at position 149 and ends at position 862. The transcript also has the following SNPs as listed in Table 10 (given according to their position on the nucleotide sequence, with the alternative nucleic acid listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein T59832_P7 (SEQ ID NO: 779) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 10 Nucleic acid SNPs SNP position on nucleotide Previously known sequence Alternative nucleic acid SNP? 61 C -> T Yes 148 G -> T Yes 212 -> A No 241 G -> T No 244 A -> G Yes 375 G -> A Yes 377 G -> A No 439 G -> C Yes 585 T -> No 586 T -> G Yes 651 C -> A No 656 C -> No 656 C -> G No 686 A -> G Yes 751 A -> G Yes 1004 T -> G Yes 1206 C -> No 1206 C -> A No 1223 C -> T Yes 1238 T -> G No 1258 G -> A No 1258 G -> C No 1294 C -> No 1294 C -> T No 1323 C -> No 1338 G -> A No 1353 G -> T Yes 1360 A -> No 1382 -> T No 1383 C -> T No 1402 C -> No 1408 A -> G Yes 1420 G -> A Yes 1463 A -> C Yes 1488 A -> C Yes 1496 T -> C Yes

Variant protein T59832_P9 (SEQ ID NO: 780) according to the present invention has an amino acid sequence as given at the end of the application; it is encoded by transcript(s) T59832_T11 (SEQ ID NO: 744). An alignment is given to the known protein (Gamma-interferon inducible lysosomal thiol reductase precursor) at the end of the application. One or more alignments to one or more previously published protein sequences are given at the end of the application. A brief description of the relationship of the variant protein according to the present invention to each such aligned protein is as follows:

Comparison report between T59832_P9 (SEQ ID NO: 780) and GILT_HUMAN:

1. An isolated chimeric polypeptide encoding for T59832_P9 (SEQ ID NO: 780), comprising a first amino acid sequence being at least 90% homologous to MTLSPLLLFLPPLLLLLDVPTAAVQASPLQALDFFGNGPPVNYKTGNLYLRGPLKKSNA PLVNVTLYYEALCGGCRAFLIRELFPTWLLVMEILNVTLVPYGNAQEQNVSGRWEFKC QHGEEECKFNKVEACVLDELDMELAFLTIVCMEEFEDMERSLPLCLQLYAPGLSPDTIM ECAMGDRGMQLMHANAQRTDALQPPHE corresponding to amino acids 12-214 of GILT_HUMAN, which also corresponds to amino acids 1-203 of T59832_P9 (SEQ ID NO: 780), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence NPWKIRPSSLPLSASCTRARSRMSALPQPAPSGVFASSDGR (SEQ ID NO: 1090) corresponding to amino acids 204-244 of T59832_P9 (SEQ ID NO: 780), wherein said first amino acid sequence and second amino acid sequence are contiguous and in a sequential order.

2. An isolated polypeptide encoding for a tail of T59832_P9 (SEQ ID NO: 780), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence NPWKIRPSSLPLSASCTRARSRMSALPQPAPSGVFASSDGR (SEQ ID NO: 1090) in T59832_P9 (SEQ ID NO: 780).

The location of the variant protein was determined according to results from a number of different software programs and analyses, including analyses from SignalP and other specialized programs. The variant protein is believed to be located as follows with regard to the cell: secreted. The protein localization is believed to be secreted because both signal-peptide prediction programs predict that this protein has a signal peptide, and neither trans-membrane region prediction program predicts that this protein has a trans-membrane region.

Variant protein T59832_P9 (SEQ ID NO: 780) also has the following non-silent SNPs (Single Nucleotide Polymorphisms) as listed in Table 11, (given according to their position(s) on the amino acid sequence, with the alternative amino acid(s) listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein T59832_P9 (SEQ ID NO: 780) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 11 Amino acid mutations SNP position(s) on amino acid Alternative Previously sequence amino acid(s) known SNP? 76 R -> Q Yes 77 A -> T No 146 I -> No 146 I -> M Yes 168 P -> Q No 170 L -> No 170 L -> V No 180 M -> V Yes 204 N -> No 204 N -> K No 210 P -> L Yes 215 L -> W No 222 A -> T No 222 A -> P No 234 P -> No 234 P -> S No 243 G -> No

The glycosylation sites of variant protein T59832_P9 (SEQ ID NO: 780), as compared to the known protein Gamma-interferon inducible lysosomal thiol reductase precursor, are described in Table 12 (given according to their position(s) on the amino acid sequence in the first column; the second column indicates whether the glycosylation site is present in the variant protein; and the last column indicates whether the position is different on the variant protein).

TABLE 12 Glycosylation site(s) Position(s) on known amino Position in acid sequence Present in variant protein? variant protein? 119 yes 108 106 yes 95 74 yes 63

Variant protein T59832_P9 (SEQ ID NO: 780) is encoded by the following transcript(s): T59832_T11 (SEQ ID NO: 744), for which the sequence(s) is/are given at the end of the application. The coding portion of transcript T59832_T11 (SEQ ID NO: 744) is shown in bold; this coding portion starts at position 149 and ends at position 880. The transcript also has the following SNPs as listed in Table 13 (given according to their position on the nucleotide sequence, with the alternative nucleic acid listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein T59832_P9 (SEQ ID NO: 780) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 13 Nucleic acid SNPs SNP position on nucleotide Previously known sequence Alternative nucleic acid SNP? 61 C -> T Yes 148 G -> T Yes 212 -> A No 241 G -> T No 244 A -> G Yes 375 G -> A Yes 377 G -> A No 439 G -> C Yes 585 T -> No 586 T -> G Yes 651 C -> A No 656 C -> No 656 C -> G No 686 A -> G Yes 751 A -> G Yes 760 C -> No 760 C -> A No 777 C -> T Yes 792 T -> G No 812 G -> A No 812 G -> C No 848 C -> No 848 C -> T No 877 C -> No 892 G -> A No 907 G -> T Yes 914 A -> No 936 -> T No 937 C -> T No 956 C -> No 962 A -> G Yes 974 G -> A Yes 1017 A -> C Yes 1042 A -> C Yes 1050 T -> C Yes

Variant protein T59832_P12 (SEQ ID NO: 781) according to the present invention has an amino acid sequence as given at the end of the application; it is encoded by transcript(s) T59832_T15 (SEQ ID NO: 745). An alignment is given to the known protein (Gamma-interferon inducible lysosomal thiol reductase precursor) at the end of the application. One or more alignments to one or more previously published protein sequences are given at the end of the application. A brief description of the relationship of the variant protein according to the present invention to each such aligned protein is as follows:

Comparison report between T59832_P12 (SEQ ID NO: 781) and GILT_HUMAN:

1. An isolated chimeric polypeptide encoding for T59832_P12 (SEQ ID NO: 781), comprising a first amino acid sequence being at least 90% homologous to MTLSPLLLFLPPLLLLLDVPTAAVQASPLQALDFFGNGPPVNYKTGNLYLRGPLKKSNA PLVNVTLYYEALCGGCRAFLIRELFPTWLLVMEILNVTLVPYGNAQEQNVSGRWEFKC QHGEEECKFNKVE corresponding to amino acids 12-141 of GILT_HUMAN, which also corresponds to amino acids 1-130 of T59832_P12 (SEQ ID NO: 781), and a second amino acid sequence being at least 90% homologous to CLQLYAPGLSPDTIMECAMGDRGMQLMHANAQRTDALQPPHEYVPWVTVNGKPLED QTQLLTLVCQLYQGKKPDVCPSSTSSLRSVCFK corresponding to amino acids 173-261 of GILT_HUMAN, which also corresponds to amino acids 131-219 of T59832_P12 (SEQ ID NO: 781), wherein said first amino acid sequence and second amino acid sequence are contiguous and in a sequential order.

2. An isolated chimeric polypeptide encoding for an edge portion of T59832_P12 (SEQ ID NO: 781), comprising a polypeptide having a length “n”, wherein n is at least about 10 amino acids in length, optionally at least about 20 amino acids in length, preferably at least about 30 amino acids in length, more preferably at least about 40 amino acids in length and most preferably at least about 50 amino acids in length, wherein at least two amino acids comprise EC, having a structure as follows: a sequence starting from any of amino acid numbers 130-x to 130; and ending at any of amino acid numbers 131+((n−2)−x), in which x varies from 0 to n−2.

The location of the variant protein was determined according to results from a number of different software programs and analyses, including analyses from SignalP and other specialized programs. The variant protein is believed to be located as follows with regard to the cell: secreted. The protein localization is believed to be secreted because both signal-peptide prediction programs predict that this protein has a signal peptide, and neither trans-membrane region prediction program predicts that this protein has a trans-membrane region.

Variant protein T59832_P12 (SEQ ID NO: 781) also has the following non-silent SNPs (Single Nucleotide Polymorphisms) as listed in Table 14, (given according to their position(s) on the amino acid sequence, with the alternative amino acid(s) listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein T59832_P12 (SEQ ID NO: 781) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 14 Amino acid mutations SNP position(s) on amino acid Alternative sequence amino acid(s) Previously known SNP? 76 R -> Q Yes 77 A -> T No 137 P -> Q No 139 L -> No 139 L -> V No 149 M -> V Yes 183 P -> No 183 P -> T No 200 G -> A No 200 G -> D No 212 S -> No 212 S -> F No

The glycosylation sites of variant protein T59832_P12 (SEQ ID NO: 781), as compared to the known protein Gamma-interferon inducible lysosomal thiol reductase precursor, are described in Table 15 (given according to their position(s) on the amino acid sequence in the first column; the second column indicates whether the glycosylation site is present in the variant protein; and the last column indicates whether the position is different on the variant protein).

TABLE 15 Glycosylation site(s) Position(s) on known amino Position in acid sequence Present in variant protein? variant protein? 119 yes 108 106 yes 95 74 yes 63

Variant protein T59832_P12 (SEQ ID NO: 781) is encoded by the following transcript(s): T59832_T15 (SEQ ID NO: 745), for which the sequence(s) is/are given at the end of the application. The coding portion of transcript T59832_T15 (SEQ ID NO: 745) is shown in bold; this coding portion starts at position 149 and ends at position 805. The transcript also has the following SNPs as listed in Table 16 (given according to their position on the nucleotide sequence, with the alternative nucleic acid listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein T59832_P12 (SEQ ID NO: 781) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 16 Nucleic acid SNPs SNP position on nucleotide Previously known sequence Alternative nucleic acid SNP? 61 C -> T Yes 148 G -> T Yes 212 -> A No 241 G -> T No 244 A -> G Yes 375 G -> A Yes 377 G -> A No 439 G -> C Yes 558 C -> A No 563 C -> No 563 C -> G No 593 A -> G Yes 658 A -> G Yes 695 C -> No 695 C -> A No 712 C -> T Yes 727 T -> G No 747 G -> A No 747 G -> C No 783 C -> No 783 C -> T No 812 C -> No 827 G -> A No 842 G -> T Yes 849 A -> No 871 -> T No 872 C -> T No 891 C -> No 897 A -> G Yes 909 G -> A Yes 952 A -> C Yes 977 A -> C Yes 985 T -> C Yes

Variant protein T59832_P18 (SEQ ID NO: 782) according to the present invention has an amino acid sequence as given at the end of the application; it is encoded by transcript(s) T59832_T22 (SEQ ID NO. 746). An alignment is given to the known protein (Gamma-interferon inducible lysosomal thiol reductase precursor) at the end of the application. One or more alignments to one or more previously published protein sequences are given at the end of the application. A brief description of the relationship of the variant protein according to the present invention to each such aligned protein is as follows:

Comparison report between T59832_P18 (SEQ ID NO: 782) and GILT_HUMAN:

1. An isolated chimeric polypeptide encoding for T59832_P18 (SEQ ID NO: 782), comprising a first amino acid sequence being at least 90% homologous to MTLSPLLLFLPPLLLLLDVPTAAVQASPLQALDFFGNGPPVNYK corresponding to amino acids 12-55 of GILT_HUMAN, which also corresponds to amino acids 1-44 of T59832_P18 (SEQ ID NO: 782), and a second amino acid sequence being at least 90% homologous to CLQLYAPGLSPDTIMECAMGDRGMQLMHANAQRTDALQPPHEYVPWVTVNGKPLED QTQLLTLVCQLYQGKKPDVCPSSTSSLRSVCFK corresponding to amino acids 173-261 of GILT_HUMAN, which also corresponds to amino acids 45-133 of T59832_P18 (SEQ ID NO: 782), wherein said first amino acid sequence and second amino acid sequence are contiguous and in a sequential order.

2. An isolated chimeric polypeptide encoding for an edge portion of T59832_P18 (SEQ ID NO: 782), comprising a polypeptide having a length “n”, wherein n is at least about 10 amino acids in length, optionally at least about 20 amino acids in length, preferably at least about 30 amino acids in length, more preferably at least about 40 amino acids in length and most preferably at least about 50 amino acids in length, wherein at least two amino acids comprise KC, having a structure as follows: a sequence starting from any of amino acid numbers 44-x to 44; and ending at any of amino acid numbers 45+((n−2)−x), in which x varies from 0 to n−2.

The location of the variant protein was determined according to results from a number of different software programs and analyses, including analyses from SignalP and other specialized programs. The variant protein is believed to be located as follows with regard to the cell: secreted. The protein localization is believed to be secreted because both signal-peptide prediction programs predict that this protein has a signal peptide, and neither trans-membrane region prediction program predicts that this protein has a trans-membrane region.

Variant protein T59832_P18 (SEQ ID NO: 782) also has the following non-silent SNPs (Single Nucleotide Polymorphisms) as listed in Table 17, (given according to their position(s) on the amino acid sequence, with the alternative amino acid(s) listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein T59832_P18 (SEQ ID NO: 782) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 17 Amino acid mutations SNP position(s) on amino acid Alternative sequence amino acid(s) Previously known SNP? 51 P -> Q No 53 L -> V No 53 L -> No 63 M -> V Yes 97 P -> No 97 P -> T No 114 G -> A No 114 G -> D No 126 S -> F No 126 S -> No

The glycosylation sites of variant protein T59832_P18 (SEQ ID NO: 782), as compared to the known protein Gamma-interferon inducible lysosomal thiol reductase precursor, are described in Table 18 (given according to their position(s) on the amino acid sequence in the first column; the second column indicates whether the glycosylation site is present in the variant protein; and the last column indicates whether the position is different on the variant protein).

TABLE 18 Glycosylation site(s) Position(s) on known amino acid sequence Present in variant protein? 119 no 106 no 74 no

Variant protein T59832_P18 (SEQ ID NO: 782) is encoded by the following transcript(s): T59832_T22 (SEQ ID NO. 746), for which the sequence(s) is/are given at the end of the application. The coding portion of transcript T59832_T22 (SEQ ID NO. 746) is shown in bold; this coding portion starts at position 149 and ends at position 547. The transcript also has the following SNPs as listed in Table 19 (given according to their position on the nucleotide sequence, with the alternative nucleic acid listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein T59832_P18 (SEQ ID NO: 782) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 19 Nucleic acid SNPs SNP position on nucleotide Previously known sequence Alternative nucleic acid SNP? 61 C -> T Yes 148 G -> T Yes 212 -> A No 241 G -> T No 244 A -> G Yes 300 C -> A No 305 C -> No 305 C -> G No 335 A -> G Yes 400 A -> G Yes 437 C -> No 437 C -> A No 454 C -> T Yes 469 T -> G No 489 G -> A No 489 G -> C No 525 C -> No 525 C -> T No 554 C -> No 569 G -> A No 584 G -> T Yes 591 A -> No 613 -> T No 614 C -> T No 633 C -> No 639 A -> G Yes 651 G -> A Yes 694 A -> C Yes 719 A -> C Yes 727 T -> C Yes

As noted above, cluster T59832 features 30 segment(s), which were listed in Table 2 above and for which the sequence(s) are given at the end of the application. These segment(s) are portions of nucleic acid sequence(s) which are described herein separately because they are of particular interest. A description of each segment according to the present invention is now provided.

Segment cluster T59832_node_(—)1 (SEQ ID NO: 747) according to the present invention is supported by 62 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): T59832_T6 (SEQ ID NO: 742), T59832_T8 (SEQ ID NO: 743), T59832_T11 (SEQ ID NO: 744), T59832_T15 (SEQ ID NO: 745) and T59832_T22 (SEQ ID NO. 746). Table 20 below describes the starting and ending position of this segment on each transcript.

TABLE 20 Segment location on transcripts Segment Segment Transcript starting ending name position position T59832_T6 1 123 (SEQ ID NO:742) T59832_T8 1 123 (SEQ ID NO:743) T59832_T11 1 123 (SEQ ID NO:744) T59832_T15 1 123 (SEQ ID NO:745) T59832_T22 1 123 (SEQ ID NO.746)

Segment cluster T59832_node_(—)7 (SEQ ID NO: 748) according to the present invention is supported by 8 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): T59832_T6 (SEQ ID NO: 742). Table 21 below describes the starting and ending position of this segment on each transcript.

TABLE 21 Segment location on transcripts Segment Segment Transcript starting ending name position position T59832_T6 281 1346 (SEQ ID NO:742)

Segment cluster T59832_node_(—)29 (SEQ ID NO: 749) according to the present invention is supported by 12 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): T59832_T8 (SEQ ID NO: 743). Table 22 below describes the starting and ending position of this segment on each transcript.

TABLE 22 Segment location on transcripts Segment Segment Transcript starting ending name position position T59832_T8 785 1202 (SEQ ID NO:743)

Segment cluster T59832_node_(—)39 (SEQ ID NO: 750)) according to the present invention is supported by 195 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): T59832_T6 (SEQ ID NO: 742), T59832_T8 (SEQ ID NO: 743), T59832_T11 (SEQ ID NO: 744), T59832_T15 (SEQ ID NO: 745) and T59832_T22 (SEQ ID NO. 746). Table 23 below describes the starting and ending position of this segment on each transcript.

TABLE 23 Segment location on transcripts Segment Segment Transcript starting ending name position position T59832_T6 2125 2178 (SEQ ID NO:742) T59832_T8 1477 1530 (SEQ ID NO:743) T59832_T11 1031 1084 (SEQ ID NO:744) T59832_T15 966 1019 (SEQ ID NO:745) T59832_T22 708 761 (SEQ ID NO.746)

According to an optional embodiment of the present invention, short segments related to the above cluster are also provided. These segments are up to about 120 bp in length, and so are included in a separate description.

Segment cluster T59832_node_(—)2 (SEQ ID NO: 751) according to the present invention is supported by 258 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): T59832_T6 (SEQ ID NO: 742), T59832_T8 (SEQ ID NO: 743), T59832_T11 (SEQ ID NO: 744), T59832_T15 (SEQ ID NO: 745) and T59832_T22 (SEQ ID NO. 746). Table 24 below describes the starting and ending position of this segment on each transcript.

TABLE 24 Segment location on transcripts Segment Segment Transcript starting ending name position position T59832_T6 124 154 (SEQ ID NO:742) T59832_T8 124 154 (SEQ ID NO:743) T59832_T11 124 154 (SEQ ID NO:744) T59832_T15 124 154 (SEQ ID NO:745) T59832_T22 124 154 (SEQ ID NO.746)

Segment cluster T59832_node_(—)3 (SEQ ID NO: 752) according to the present invention can be found in the following transcript(s): T59832_T6 (SEQ ID NO: 742), T59832_T8 (SEQ ID NO: 743), T59832_T11 (SEQ ID NO: 744), T59832_T15 (SEQ ID NO: 745) and T59832_T22 (SEQ ID NO. 746). Table 25 below describes the starting and ending position of this segment on each transcript.

TABLE 25 Segment location on transcripts Segment Segment Transcript starting ending name position position T59832_T6 155 172 (SEQ ID NO:742) T59832_T8 155 172 (SEQ ID NO:743) T59832_T11 155 172 (SEQ ID NO:744) T59832_T15 155 172 (SEQ ID NO:745) T59832_T22 155 172 (SEQ ID NO.746)

Segment cluster T59832_node_(—)4 (SEQ ID NO: 753) according to the present invention is supported by 296 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): T59832_T6 (SEQ ID NO: 742), T59832_T8 (SEQ ID NO: 743), T59832_T11 (SEQ ID NO: 744), T59832_T15 (SEQ ID NO: 745) and T59832_T22 (SEQ ID NO. 746). Table 26 below describes the starting and ending position of this segment on each transcript.

TABLE 26 Segment location on transcripts Segment Segment Transcript starting ending name position position T59832_T6 173 223 (SEQ ID NO:742) T59832_T8 173 223 (SEQ ID NO:743) T59832_T11 173 223 (SEQ ID NO:744) T59832_T15 173 223 (SEQ ID NO:745) T59832_T22 173 223 (SEQ ID NO.746)

Segment cluster T59832_node_(—)5 (SEQ ID NO: 754) according to the present invention is supported by 305 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): T59832_T6 (SEQ ID NO: 742), T59832_T8 (SEQ ID NO: 743), T59832_T11 (SEQ ID NO: 744), T59832_T15 (SEQ ID NO: 745) and T59832_T22 (SEQ ID NO. 746). Table 27 below describes the starting and ending position of this segment on each transcript.

TABLE 27 Segment location on transcripts Segment Segment Transcript starting ending name position position T59832_T6 224 259 (SEQ ID NO:742) T59832_T8 224 259 (SEQ ID NO:743) T59832_T11 224 259 (SEQ ID NO:744) T59832_T15 224 259 (SEQ ID NO:745) T59832_T22 224 259 (SEQ ID NO.746)

Segment cluster T59832_node_(—)6 (SEQ ID NO: 755) according to the present invention can be found in the following transcript(s): T59832_T6 (SEQ ID NO: 742), T59832_T8 (SEQ ID NO: 743), T59832_T11 (SEQ ID NO: 744), T59832_T15 (SEQ ID NO: 745) and T59832_T22 (SEQ ID NO. 746). Table 28 below describes the starting and ending position of this segment on each transcript.

TABLE 28 Segment location on transcripts Segment Segment Transcript starting ending name position position T59832_T6 260 280 (SEQ ID NO:742) T59832_T8 260 280 (SEQ ID NO:743) T59832_T11 260 280 (SEQ ID NO:744) T59832_T15 260 280 (SEQ ID NO:745) T59832_T22 260 280 (SEQ ID NO.746)

Segment cluster T59832_node_(—)8 (SEQ ID NO: 756) according to the present invention can be found in the following transcript(s): T59832_T6 (SEQ ID NO: 742), T59832_T8 (SEQ ID NO: 743), T59832T11 (SEQ ID NO: 744) and T59832_T15 (SEQ ID NO: 745). Table 29 below describes the starting and ending position of this segment on each transcript.

TABLE 29 Segment location on transcripts Segment Segment Transcript starting ending name position position T59832_T6 1347 1367 (SEQ ID NO:742) T59832_T8 281 172 (SEQ ID NO:743) T59832_T11 281 172 (SEQ ID NO:744) T59832_T15 281 172 (SEQ ID NO:745)

Segment cluster T59832_node_(—)9 (SEQ ID NO: 757) according to the present invention is supported by 330 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): T59832_T6 (SEQ ID NO: 742), T59832_T8 (SEQ ID NO: 743), T59832_T11 (SEQ ID NO: 744) and T59832_T15 (SEQ ID NO: 745). Table 30 below describes the starting and ending position of this segment on each transcript.

TABLE 30 Segment location on transcripts Segment Segment Transcript starting ending name position position T59832_T6 1368 1403 (SEQ ID NO:742) T59832_T8 302 337 (SEQ ID NO:743) T59832_T11 302 337 (SEQ ID NO:744) T59832_T15 302 337 (SEQ ID NO:745)

Segment cluster T59832_node_(—)10 (SEQ ID NO: 758) according to the present invention is supported by 332 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): T59832_T6 (SEQ ID NO: 742), T59832_T8 (SEQ ID NO: 743), T59832_T11 (SEQ ID NO: 744) and T59832_T15 (SEQ ID NO: 745). Table 31 below describes the starting and ending position of this segment on each transcript.

TABLE 31 Segment location on transcripts Segment Segment Transcript starting ending name position position T59832_T6 1404 1448 (SEQ ID NO:742) T59832_T8 338 382 (SEQ ID NO:743) T59832_T11 338 382 (SEQ ID NO:744) T59832_T15 338 382 (SEQ ID NO:745)

Segment cluster T59832_node_(—)11 (SEQ ID NO: 759) according to the present invention is supported by 306 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): T59832_T6 (SEQ ID NO: 742), T59832_T8 (SEQ ID NO: 743), T59832_T11 (SEQ ID NO: 744) and T59832_T15 (SEQ ID NO: 745). Table 32 below describes the starting and ending position of this segment on each transcript.

TABLE 32 Segment location on transcripts Segment Segment Transcript starting ending name position position T59832_T6 1449 1483 (SEQ ID NO:742) T59832_T8 383 417 (SEQ ID NO:743) T59832_T11 383 417 (SEQ ID NO:744) T59832_T15 383 417 (SEQ ID NO:745)

Segment cluster T59832_node_(—)12 (SEQ ID NO. 760) according to the present invention is supported by 280 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): T59832_T6 (SEQ ID NO: 742), T59832_T8 (SEQ ID NO: 743), T59832_T11 (SEQ ID NO: 744) and T59832_T15 (SEQ ID NO: 745). Table 33 below describes the starting and ending position of this segment on each transcript.

TABLE 33 Segment location on transcripts Segment Segment Transcript starting ending name position position T59832_T6 1484 1529 (SEQ ID NO:742) T59832_T8 418 463 (SEQ ID NO:743) T59832_T11 418 463 (SEQ ID NO:744) T59832_T15 418 463 (SEQ ID NO:745)

Segment cluster T59832_node_(—)14 (SEQ ID NO: 761) according to the present invention is supported by 280 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): T59832_T6 (SEQ ID NO: 742), T59832_T8 (SEQ ID NO: 743), T59832_T11 (SEQ ID NO: 744) and T59832_T15 (SEQ ID NO: 745). Table 34 below describes the starting and ending position of this segment on each transcript.

TABLE 34 Segment location on transcripts Segment Segment Transcript starting ending name position position T59832_T6 1530 1568 (SEQ ID NO:742) T59832_T8 464 502 (SEQ ID NO:743) T59832_T11 464 502 (SEQ ID NO:744) T59832_T15 464 502 (SEQ ID NO:745)

Segment cluster T59832_node_(—)16 (SEQ ID NO: 762) according to the present invention is supported by 287 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): T59832_T6 (SEQ ID NO: 742), T59832_T8 (SEQ ID NO: 743), T59832_T11 (SEQ ID NO: 744) and T59832_T15 (SEQ ID NO: 745). Table 35 below describes the starting and ending position of this segment on each transcript.

TABLE 35 Segment location on transcripts Segment Segment Transcript starting ending name position position T59832_T6 1569 1604 (SEQ ID NO:742) T59832_T8 503 538 (SEQ ID NO:743) T59832_T11 503 538 (SEQ ID NO:744) T59832_T15 503 538 (SEQ ID NO:745)

Segment cluster T59832_node_(—)19 (SEQ ID NO: 763) according to the present invention is supported by 300 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): T59832_T6 (SEQ ID NO: 742), T59832_T8 (SEQ ID NO: 743) and T59832_T11 (SEQ ID NO: 744). Table 36 below describes the starting and ending position of this segment on each transcript.

TABLE 36 Segment location on transcripts Segment Segment Transcript starting ending name position position T59832_T6 1605 1643 (SEQ ID NO:742) T59832_T8 539 577 (SEQ ID NO:743) T59832_T11 539 577 (SEQ ID NO:744)

Segment cluster T59832_node_(—)20 (SEQ ID NO: 764) according to the present invention is supported by 318 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): T59832_T6 (SEQ ID NO: 742), T59832_T8 (SEQ ID NO: 743) and T59832_T11 (SEQ ID NO: 744). Table 37 below describes the starting and ending position of this segment on each transcript.

TABLE 37 Segment location on transcripts Segment Segment Transcript starting ending name position position T59832_T6 1644 1697 (SEQ ID NO:742) T59832_T8 578 631 (SEQ ID NO:743) T59832_T11 578 631 (SEQ ID NO:744)

Segment cluster T59832_node_(—)25 (SEQ ID NO: 765) according to the present invention can be found in the following transcript(s): T59832_T6 (SEQ ID NO: 742), T59832_T8 (SEQ ID NO: 743), T59832_T11 (SEQ ID NO: 744), T59832_T15 (SEQ ID NO: 745) and T59832_T22 (SEQ ID NO. 746). Table 38 below describes the starting and ending position of this segment on each transcript.

TABLE 38 Segment location on transcripts Segment Segment Transcript starting ending name position position T59832_T6 1698 1719 (SEQ ID NO:742) T59832_T8 632 653 (SEQ ID NO:743) T59832_T11 632 653 (SEQ ID NO:744) T59832_T15 539 560 (SEQ ID NO:745) T59832_T22 281 302 (SEQ ID NO.746)

Segment cluster T59832_node_(—)26 (SEQ ID NO: 766) according to the present invention is supported by 342 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): T59832_T6 (SEQ ID NO: 742), T59832_T8 (SEQ ID NO: 743), T59832_T11 (SEQ ID NO: 744), T59832_T15 (SEQ ID NO: 745) and T59832_T22 (SEQ ID NO. 746). Table 39 below describes the starting and ending position of this segment on each transcript.

TABLE 39 Segment location on transcripts Segment Segment Transcript starting ending name position position T59832_T6 1720 1783 (SEQ ID NO:742) T59832_T8 654 717 (SEQ ID NO:743) T59832_T11 654 717 (SEQ ID NO:744) T59832_T15 561 624 (SEQ ID NO:745) T59832_T22 303 366 (SEQ ID NO.746)

Segment cluster T59832_node_(—)27 (SEQ ID NO: 767) according to the present invention is supported by 314 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): T59832_T6 (SEQ ID NO: 742), T59832_T8 (SEQ ID NO: 743), T59832_T11 (SEQ ID NO: 744), T59832_T15 (SEQ ID NO: 745) and T59832_T22 (SEQ ID NO. 746). Table 40 below describes the starting and ending position of this segment on each transcript.

TABLE 40 Segment location on transcripts Segment Segment Transcript starting ending name position position T59832_T6 1784 1822 (SEQ ID NO:742) T59832_T8 718 756 (SEQ ID NO:743) T59832_T11 718 756 (SEQ ID NO:744) T59832_T15 625 663 (SEQ ID NO:745) T59832_T22 367 405 (SEQ ID NO.746)

Segment cluster T59832_node_(—)28 (SEQ ID NO: 768) according to the present invention is supported by 284 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): T59832_T6 (SEQ ID NO: 742), T59832_T8 (SEQ ID NO: 743), T59832_T15 (SEQ ID NO: 745) and T59832_T22 (SEQ ID NO. 746). Table 41 below describes the starting and ending position of this segment on each transcript.

TABLE 41 Segment location on transcripts Segment Segment Transcript starting ending name position position T59832_T6 1823 1850 (SEQ ID NO:742) T59832_T8 757 784 (SEQ ID NO:743) T59832_T15 664 691 (SEQ ID NO:745) T59832_T22 406 433 (SEQ ID NO.746)

Segment cluster T59832_node_(—)30 (SEQ ID NO: 769) according to the present invention can be found in the following transcript(s): T59832_T6 (SEQ ID NO: 742), T59832_T8 (SEQ ID NO: 743), T59832_T11 (SEQ ID NO: 744), T59832_T15 (SEQ ID NO: 745) and T59832_T22 (SEQ ID NO. 746). Table 42 below describes the starting and ending position of this segment on each transcript.

TABLE 42 Segment location on transcripts Segment Segment Transcript starting ending name position position T59832_T6 1851 1854 (SEQ ID NO:742) T59832_T8 1203 1206 (SEQ ID NO:743) T59832_T11 757 760 (SEQ ID NO:744) T59832_T15 692 695 (SEQ ID NO:745) T59832_T22 434 437 (SEQ ID NO.746)

Segment cluster T59832_node_(—)31 (SEQ ID NO: 770) according to the present invention can be found in the following transcript(s): T59832_T6 (SEQ ID NO: 742), T59832_T8 (SEQ ID NO: 743), T59832_T11 (SEQ ID NO: 744), T59832_T15 (SEQ ID NO: 745) and T59832_T22 (SEQ ID NO. 746). Table 43 below describes the starting and ending position of this segment on each transcript.

TABLE 43 Segment location on transcripts Segment Segment Transcript starting ending name position position T59832_T6 1855 1874 (SEQ ID NO:742) T59832_T8 1207 1226 (SEQ ID NO:743) T59832_T11 761 780 (SEQ ID NO:744) T59832_T15 696 715 (SEQ ID NO:745) T59832_T22 438 457 (SEQ ID NO.746)

Segment cluster T59832_node_(—)32 (SEQ ID NO: 771) according to the present invention is supported by 287 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): T59832_T6 (SEQ ID NO: 742), T59832_T8 (SEQ ID NO: 743), T59832_T11 (SEQ ID NO: 744), T59832_T15 (SEQ ID NO: 745) and T59832_T22 (SEQ ID NO. 746). Table 44 below describes the starting and ending position of this segment on each transcript.

TABLE 44 Segment location on transcripts Segment Segment Transcript starting ending name position position T59832_T6 1875 1904 (SEQ ID NO:742) T59832_T8 1227 1256 (SEQ ID NO:743) T59832_T11 781 810 (SEQ ID NO:744) T59832_T15 716 745 (SEQ ID NO:745) T59832_T22 458 487 (SEQ ID NO.746)

Segment cluster T59832_node_(—)34 (SEQ ID NO: 772) according to the present invention can be found in the following transcript(s): T59832_T6 (SEQ ID NO: 742), T59832_T8 (SEQ ID NO: 743), T59832_T11 (SEQ ID NO: 744), T59832_T15 (SEQ ID NO: 745) and T59832_T22 (SEQ ID NO. 746). Table 45 below describes the starting and ending position of this segment on each transcript.

TABLE 45 Segment location on transcripts Segment Segment Transcript starting ending name position position T59832_T6 1905 1926 (SEQ ID NO:742) T59832_T8 1257 1278 (SEQ ID NO:743) T59832_T11 811 832 (SEQ ID NO:744) T59832_T15 746 767 (SEQ ID NO:745) T59832_T22 488 509 (SEQ ID NO.746)

Segment cluster T59832_node_(—)35 (SEQ ID NO: 773) according to the present invention can be found in the following transcript(s): T59832_T6 (SEQ ID NO: 742), T59832_T8 (SEQ ID NO: 743), T59832_T11 (SEQ ID NO: 744), T59832_T15 (SEQ ID NO: 745) and T59832_T22 (SEQ ID NO. 746). Table 46 below describes the starting and ending position of this segment on each transcript.

TABLE 46 Segment location on transcripts Segment Segment Transcript starting ending name position position T59832_T6 1927 1930 (SEQ ID NO:742) T59832_T8 1279 1282 (SEQ ID NO:743) T59832_T11 833 836 (SEQ ID NO:744) T59832_T15 768 771 (SEQ ID NO:745) T59832_T22 510 513 (SEQ ID NO:746)

Segment cluster T59832_node_(—)36 (SEQ ID NO: 774) according to the present invention can be found in the following transcript(s): T59832_T6 (SEQ ID NO: 742), T59832_T8 (SEQ ID NO: 743), T59832_T11 (SEQ ID NO: 744), T59832_T15 (SEQ ID NO: 745) and T59832_T22 (SEQ ID NO. 746). Table 47 below describes the starting and ending position of this segment on each transcript.

TABLE 47 Segment location on transcripts Segment Segment Transcript starting ending name position position T59832_T6 1931 1939 (SEQ ID NO:742) T59832_T8 1283 1291 (SEQ ID NO:743) T59832_T11 837 845 (SEQ ID NO:744) T59832_T15 772 780 (SEQ ID NO:745) T59832_T22 514 522 (SEQ ID NO:746)

Segment cluster T59832_node_(—)37 (SEQ ID NO: 775) according to the present invention is supported by 300 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): T59832_T6 (SEQ ID NO: 742), T59832_T8 (SEQ ID NO: 743), T59832_T11 (SEQ ID NO: 744), T59832_T15 (SEQ ID NO: 745) and T59832_T22 (SEQ ID NO. 746). Table 48 below describes the starting and ending position of this segment on each transcript.

TABLE 48 Segment location on transcripts Segment Segment Transcript starting ending name position position T59832_T6 1940 2039 (SEQ ID NO:742) T59832_T8 1292 1391 (SEQ ID NO:743) T59832_T11 846 945 (SEQ ID NO:744) T59832_T15 781 880 (SEQ ID NO:745) T59832_T22 523 622 (SEQ ID NO:746)

Segment cluster T59832_node_(—)38 (SEQ ID NO: 776) according to the present invention is supported by 247 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): T59832_T6 (SEQ ID NO: 742), T59832_T8 (SEQ ID NO: 743), T59832_T11 (SEQ ID NO: 744), T59832_T15 (SEQ ID NO: 745) and T59832_T22 (SEQ ID NO. 746). Table 49 below describes the starting and ending position of this segment on each transcript.

TABLE 49 Segment location on transcripts Segment Segment Transcript starting ending name position position T59832_T6 1940 2039 (SEQ ID NO:742) T59832_T8 1292 1391 (SEQ ID NO:743) T59832_T11 846 945 (SEQ ID NO:744) T59832_T15 781 880 (SEQ ID NO:745) T59832_T22 523 622 (SEQ ID NO:746) Variant protein alignment to the previously known protein: Sequence name: GILT_HUMAN Sequence documentation:

Alignment of: T59832_P7 (SEQ ID NO: 779) x GILT_HUMAN ..

Alignment segment 1/1:

Quality: 2110.00 Escore: 0 Matching length: 212 Total length: 212 Matching Percent Similarity: 100.00 Matching Percent Identity: 100.00 Total Percent Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0

Alignment:

Sequence name: GILT_HUMAN Sequence documentation:

Alignment of: T59832_P9 (SEQ ID NO: 780) x GILT_HUMAN ..

Alignment segment 1/1:

Quality: 2016.00 Escore: 0 Matching length: 203 Total length: 203 Matching Percent Similarity: 100.00 Matching Percent Identity: 100.00 Total Percent Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0

Alignment:

Sequence name: GILT_HUMAN Sequence documentation:

Alignment of: T59832_P12 (SEQ ID NO: 781) x GILT_HUMAN ..

Alignment segment 1/1:

Quality: 2084.00 Escore: 0 Matching length: 219 Total length: 250 Matching Percent Similarity: 100.00 Matching Percent Identity: 100.00 Total Percent Similarity: 87.60 Total Percent Identity: 87.60 Gaps: 1

Alignment:

Sequence name: GILT_HUMAN Sequence documentation:

Alignment of: T59832_P18 (SEQ ID NO: 782) x GILT_HUMAN ..

Alignment segment 1/1:

Quality: 1222.00 Escore: 0 Matching length: 133 Total length: 250 Matching Percent Similarity: 100.00 Matching Percent Identity: 100.00 Total Percent Similarity: 53.20 Total Percent Identity: 53.20 Gaps: 1

Alignment:

Expression of Homo sapiens Interferon, Gamma-Inducible Protein 30 (IFI30) T59832 Transcripts Which are Detectable by Amplicon as Depicted in Sequence Name T59832 junc6-25-26 (SEQ ID NO:1010) in Normal and Cancerous Ovary Tissues

Expression of Homo sapiens interferon, gamma-inducible protein 30 (IFI30) transcripts detectable by or according to junc6-25-26, T59832 junc6-25-26 (SEQ ID NO:1010) amplicon(s) and primers T59832 junc6-25-26F (SEQ ID NO:1008) and T59832 junc6-25-26R (SEQ ID NO:1009) was measured by real time PCR. In parallel the expression of four housekeeping genes —PBGD (GenBank Accession No. BC019323 (SEQ ID NO:1036); amplicon—PBGD-amplicon (SEQ ID NO:1039)), HPRT1 (GenBank Accession No. NM_(—)000194 (SEQ ID NO: 1040); amplicon—HPRT1-amplicon (SEQ ID NO: 1044)), SDHA (GenBank Accession No. NM_(—)004168 (SEQ ID NO:1032); amplicon—SDHA-amplicon (SEQ ID NO:1035)), and GAPDH (GenBank Accession No. BC026907; GAPDH amplicon (SEQ ID NO:1047)) was measured similarly. For each RT sample, the expression of the above amplicon was normalized to the geometric mean of the quantities of the housekeeping genes. The normalized quantity of each RT sample was then divided by the median of the quantities of the normal post-mortem (PM) samples (Sample Nos. 45-48, 71, Table 1, above), to obtain a value of fold differential expression for each sample relative to median of the normal PM samples.

In one experiment that was carried out no differential expression in the cancerous samples relative to the normal PM samples was observed, although this may be due a problem with this specific experiment.

Primer pairs are also optionally and preferably encompassed within the present invention; for example, for the above experiment, the following primer pair was used as a non-limiting illustrative example only of a suitable primer pair: T59832 junc6-25-26F forward primer (SEQ ID NO:1008); and T59832 junc6-25-26R reverse primer (SEQ ID NO:1009).

The present invention also preferably encompasses any amplicon obtained through the use of any suitable primer pair; for example, for the above experiment, the following amplicon was obtained as a non-limiting illustrative example only of a suitable amplicon: T59832 junc6-25-26 (SEQ ID NO:1010).

Forward primer T59832 junc6-25-26F (SEQ ID NO:1008): CCACCAGTTAACTACAAGTGCCTG Reverse primer T59832 junc6-25-26R (SEQ ID NO:1009): GCGTGCATGAGCTGCATG Amplicon T59832 junc6-25-26 (SEQ ID NO:1010): CCACCAGTTAACTACAAGTGCCTGCAGCTCTACGCCCCAGGGCTGTCGCC AGACACTATCATGGAGTGTGCAATGGGGGACCGCGGCATGCAGCTCATGC ACGC

Description for Cluster HSCP2

Cluster HSCP2 features 12 transcript(s) and 50 segment(s) of interest, the names for which are given in Tables 1 and 2, respectively, the sequences themselves are given at the end of the application. The selected protein variants are given in table 3.

TABLE 1 Transcripts of interest Transcript Name SEQ ID NO: HSCP2_PEA_1_T4 783 HSCP2_PEA_1_T13 784 HSCP2_PEA_1_T19 785 HSCP2_PEA_1_T20 786 HSCP2_PEA_1_T22 787 HSCP2_PEA_1_T23 788 HSCP2_PEA_1_T25 789 HSCP2_PEA_1_T31 790 HSCP2_PEA_1_T33 791 HSCP2_PEA_1_T34 792 HSCP2_PEA_1_T45 793 HSCP2_PEA_1_T50 794

TABLE 2 Segments of interest Segment Name SEQ ID NO: HSCP2_PEA_1_node_0 795 HSCP2_PEA_1_node_3 796 HSCP2_PEA_1_node_6 797 HSCP2_PEA_1_node_8 798 HSCP2_PEA_1_node_10 799 HSCP2_PEA_1_node_14 800 HSCP2_PEA_1_node_23 801 HSCP2_PEA_I node_26 802 HSCP2_PEA_1_node_29 803 HSCP2_PEA_1_node_31 804 HSCP2_PEA_1_node_32 805 HSCP2_PEA_1_node_34 806 HSCP2_PEA_1_node_52 807 HSCP2_PEA_1_node_58 808 HSCP2_PEA_1_node_72 809 HSCP2_PEA_1_node_73 810 HSCP2_PEA_1_node_74 811 HSCP2_PEA_1_node_76 812 HSCP2_PEA_1_node_78 813 HSCP2_PEA_1_node_80 814 HSCP2_PEA_1_node_84 815 HSCP2_PEA_1_node_4 816 HSCP2_PEA_1_node_7 817 HSCP2_PEA_1_node_13 818 HSCP2_PEA_1_node_15 819 HSCP2_PEA_1_node_16 820 HSCP2_PEA_1_node_18 821 HSCP2_PEA_1_node_20 822 HSCP2_PEA_1_node_21 823 HSCP2_PEA_1_node_37 824 HSCP2_PEA_1_node_38 825 HSCP2_PEA_1_node_39 826 HSCP2_PEA_1_node_41 827 HSCP2_PEA_1_node_42 828 HSCP2_PEA_1_node_46 829 HSCP2_PEA_1_node_47 830 HSHSCP2_PEA_1_node_50 831 HSCP2_PEA_1_node_51 832 HSCP2_PEA_1_node_55 833 HSCP2_PEA_1_node_56 834 HSCP2_PEA_1_node_60 835 HSCP2_PEA_1_node_61 836 HSCP2_PEA_1_node_67 837 HSCP2_PEA_1_node_68 838 HSCP2_PEA_1_node_69 839 HSCP2_PEA_1_node_70 840 HSCP2_PEA_1_node_75 841 HSCP2_PEA_1_node_77 842 HSCP2_PEA_1_node_79 843 HSCP2_PEA_1_node_82 844

TABLE 3 Proteins of interest SEQ ID Corresponding Protein Name NO: Transcript(s) HSCP2_PEA_1_P4 846 HSCP2_PEA_1_T4 (SEQ ID NO:783); HSCP2_PEA_1_T50 (SEQ ID NO:794) HSCP2_PEA_1_P8 847 HSCP2_PEA_1_T13 (SEQ ID NO:784) HSCP2_PEA_1_P14 848 HSCP2_PEA_1_T19 (SEQ ID NO:785) HSCP2_PEA_1_P15 849 HSCP2_PEA_1_T20 (SEQ ID NO:786) HSCP2_PEA_1_P2 850 HSCP2_PEA_1_T22 (SEQ ID NO:787) HSCP2_PEA_1_P16 851 HSCP2_PEA_1_T23 (SEQ ID NO:788) HSCP2_PEA_1_P6 852 HSCP2_PEA_1_T25 (SEQ ID NO:789) HSCP2_PEA_1_P22 853 HSCP2_PEA_1_T31 (SEQ ID NO:790) HSCP2_PEA_1_P24 854 HSCP2_PEA_1_T33 (SEQ ID NO:791) HSCP2_PEA_1_P25 855 HSCP2_PEA_1_T34 (SEQ ID NO:792) HSCP2_PEA_1_P33 856 HSCP2_PEA_1_T45 (SEQ ID NO:793)

These sequences are variants of the known protein Ceruloplasmin precursor (SwissProt accession identifier CERU_HUMAN; known also according to the synonyms EC 1.16.3.1; Ferroxidase), SEQ ID NO: 845, referred to herein as the previously known protein.

Protein Ceruloplasmin precursor is known or believed to have the following function(s): Ceruloplasmin is a blue, copper-binding (6-7 atoms per molecule) glycoprotein found in plasma. Four possible functions are ferroxidase activity, amine oxidase activity, copper transport and homeostasis, and superoxide dismutase activity. The sequence for protein Ceruloplasmin precursor is given at the end of the application, as “Ceruloplasmin precursor amino acid sequence”. Known polymorphisms for this sequence are as shown in Table 4.

TABLE 4 Amino acid mutations for Known Protein SNP position(s) on amino acid sequence Comment 79 T -> G. /FTId = VAR_001043. 449 L -> G. /FTId = VAR_001044. 1060 E -> EGEYP

The following GO Annotation(s) apply to the previously known protein. The following annotation(s) were found: ion transport; copper ion transport; copper homeostasis; iron homeostasis, which are annotation(s) related to Biological Process; ferroxidase; copper ion transporter; copper binding; oxidoreductase, which are annotation(s) related to Molecular Function; and extracellular space, which are annotation(s) related to Cellular Component.

The GO assignment relies on information from one or more of the SwissProt/TremBl Protein knowledgebase, available from <http://www.expasy.ch/sprot/>; or Locuslink, available from <http://www.ncbi.nlm.nih.gov/projects/LocusLink/>.

Cluster HSCP2 can be used as a diagnostic marker according to overexpression of transcripts of this cluster in cancer. Expression of such transcripts in normal tissues is also given according to the previously described methods. The term “number” in the left hand column of the table and the numbers on the y-axis of FIG. 36 refer to weighted expression of ESTs in each category, as “parts per million” (ratio of the expression of ESTs for a particular cluster to the expression of all ESTs in that category, according to parts per million).

Overall, the following results were obtained as shown with regard to the histograms in FIG. 36 and Table 5. This cluster is overexpressed (at least at a minimum level) in the following pathological conditions: kidney malignant tumors and ovarian carcinoma.

TABLE 5 Normal tissue distribution Name of Tissue Number bladder 0 bone 9 brain 48 epithelial 100 general 58 head and neck 0 kidney 4 liver 1818 lung 96 lymph nodes 18 breast 43 bone marrow 0 ovary 0 pancreas 10 prostate 6 Thyroid 0 uterus 113

TABLE 6 P values and ratios for expression in cancerous tissue Name of Tissue P1 P2 SP1 R3 SP2 R4 bladder 5.4e−01 6.0e−01 5.6e−01 1.8 6.8e−01 1.5 bone 6.3e−01 8.3e−01 1 1.0 7.0e−01 1.2 brain 8.1e−01 8.4e−01 9.8e−01 0.3 1 0.2 epithelial 2.5e−01 5.8e−01 1.9e−03 1.3 2.4e−01 0.9 general 4.0e−01 7.6e−01 1.0e−08 1.8 7.4e−04 1.2 head and neck 2.1e−01 3.3e−01 2.1e−01 4.3 5.6e−01 1.9 kidney 4.0e−01 4.4e−01 2.9e−04 8.5 2.3e−03 6.1 liver 2.9e−01 8.3e−01 1 0.3 1 0.1 lung 8.4e−01 9.0e−01 4.4e−02 1.1 5.6e−01 0.6 lymph nodes 5.8e−01 8.2e−01 4.9e−01 1.8 8.2e−01 0.9 breast 3.2e−01 3.7e−01 2.3e−01 2.1 5.7e−01 1.3 bone marrow 1 6.7e−01 1 1.0 5.3e−01 1.9 ovary 7.8e−03 7.0e−03 7.0e−04 7.5 4.9e−03 5.6 pancreas 2.3e−01 4.0e−01 1.2e−03 2.5 9.4e−03 1.8 prostate 9.7e−01 9.3e−01 1 0.8 7.4e−05 1.3 Thyroid 5.0e−01 5.0e−01 6.7e−01 1.5 6.7e−01 1.5 Uterus 2.4e−01 1.7e−01 6.5e−04 2.1 7.2e−02 1.3

As noted above, cluster HSCP2 features 12 transcript(s), which were listed in Table 1 above. These transcript(s) encode for protein(s) which are variant(s) of protein Ceruloplasmin precursor. A description of each variant protein according to the present invention is now provided.

Variant protein HSCP2_PEA_(—)1_P4 (SEQ ID NO: 846) according to the present invention has an amino acid sequence as given at the end of the application; it is encoded by transcript(s) HSCP2_PEA_(—)1_T4 (SEQ ID NO: 783) and HSCP2_PEA_(—)1_T50 (SEQ ID NO: 794). An alignment is given to the known protein (Ceruloplasmin precursor) at the end of the application. One or more alignments to one or more previously published protein sequences are given at the end of the application. A brief description of the relationship of the variant protein according to the present invention to each such aligned protein is as follows:

Comparison report between HSCP2_PEA_(—)1_P4 (SEQ ID NO: 846) and CERU_HUMAN:

1. An isolated chimeric polypeptide encoding for HSCP2_PEA_(—)1_P4 (SEQ ID NO: 846), comprising a first amino acid sequence being at least 90% homologous to MKILILGIFLFLCSTPAWAKEKHYYIGIIETTWDYASDHGEKKLISVDTEHSNIYLQNGPD RIGRLYKKALYLQYTDETFRTTIEKPVWLGFLGPIIKAETGDKVYVHLKNLASRPYTFHS HGITYYKEHEGAIYPDNTTDFQRADDKVYPGEQYTYMLLATEEQSPGEGDGNCVTRIY HSHIDAPKDIASGLIGPLIICKKDSLDKEKEKHIDREFVVMFSVVDENFSWYLEDNIKTY CSEPEKVDKDNEDFQESNRMYSVNGYTFGSLPGLSMCAEDRVKWYLFGMGNEVDVH AAFFHGQALTNKNYRIDTINLFPATLFDAYMVAQNPGEWMLSCQNLNHLKAGLQAFF QVQECNKSSSKDNIRGKHVRHYYIAAEEIIWNYAPSGIDIFTKENLTAPGSDSAVFFEQG TTRIGGSYKKLVYREYTDASFTNRKERGPEEEHLGILGPVIWAEVGDTIRVTFHNKGAY PLSIEPIGVRFNKNNEGTYYSPNYNPQSRSVPPSASHVAPTETFTYEWTVPKEVGPTNAD PVCLAKMYYSAVDPTKDIFTGLIGPMKICKKGSLHANGRQKDVDKEFYLFPTVFDENES LLLEDNIRMFTTAPDQVDKEDEDFQESNKMHSMNGFMYGNQPGLTMCKGDSVVWYL FSAGNEADVHGIYFSGNTYLWRGERRDTANLFPQTSLTLHMWPDTEGTFNVECLTTDH YTGGMKQKYTVNQCRRQSEDSTFYLGERTYYIAAVEVEWDYSPQREWEKELHHLQEQ NVSNAFLDKGEFYIGSKYKKVVYRQYTDSTFRVPVERKAEEEHLGILGPQLHADVGDK VKIIFKNMATRPYSIHAHGVQTESSTVTPTLPGETLTYVWKIPERSGAGTEDSACIPWAY YSTVDQVKDLYSGLIGPLIVCRRPYLKVFNPRRKLEFALLFLVFDENESWYLDDNIKTYS DHPEKVNKDDEEFIESNKMHAINGRMFGNLQGLTMHVGDEVNWYLMGMGNEIDLHT VHFHGHSFQYKHRGVYSSDVFDIFPGTYQTLEMFPRTPGIWLLHCHVTDHIHAGMETT YTVLQNE corresponding to amino acids 1-1060 of CERU_HUMAN, which also corresponds to amino acids 1-1060 of HSCP2_PEA_(—)1_P4 (SEQ ID NO: 846), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence GGTSM (SEQ ID NO: 1091) corresponding to amino acids 1061-1065 of HSCP2_PEA_(—)1_P4 (SEQ ID NO: 846), wherein said first amino acid sequence and second amino acid sequence are contiguous and in a sequential order.

2. An isolated polypeptide encoding for a tail of HSCP2_PEA_(—)1_P4 (SEQ ID NO: 846), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence GGTSM (SEQ ID NO: 1091) in HSCP2_PEA_(—)1_P4 (SEQ ID NO: 846).

The location of the variant protein was determined according to results from a number of different software programs and analyses, including analyses from SignalP and other specialized programs. The variant protein is believed to be located as follows with regard to the cell: secreted. The protein localization is believed to be secreted because both signal-peptide prediction programs predict that this protein has a signal peptide, and neither trans-membrane region prediction program predicts that this protein has a trans-membrane region.

Variant protein HSCP2_PEA_(—)1_P4 (SEQ ID NO: 846) also has the following non-silent SNPs (Single Nucleotide Polymorphisms) as listed in Table 7 (given according to their position(s) on the amino acid sequence, with the alternative amino acid(s) listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein HSCP2_PEA_(—)1_P4 (SEQ ID NO: 846) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 7 Amino acid mutations SNP position(s) on amino acid Alternative sequence amino acid(s) Previously known SNP? 26 I -> No 29 I -> No 37 S -> P No 47 V -> No 54 I -> V No 63 I -> No 92 F -> S No 117 Y -> N No 148 K -> R No 173 N -> No 186 P -> No 190 A -> No 190 A -> G No 213 I -> No 218 V -> M No 221 F -> No 235 N -> D No 253 F -> L No 275 M -> T No 286 F -> L No 298 F -> S No 305 T -> A No 445 H -> Y No 451 P -> A No 477 P -> L No 493 P -> No 507 S -> P No 535 L -> P No 544 D -> E Yes 584 V -> A No 598 R -> K Yes 607 V -> G Yes 640 D -> G No 660 F -> S No 675 A -> No 711 Q -> No 727 F -> S No 748 Q -> No 759 Q -> No 759 Q -> P No 789 D -> N No 927 E -> K Yes 1040 C -> W No

The glycosylation sites of variant protein HSCP2_PEA_(—)1_P4 (SEQ ID NO: 846), as compared to the known protein Ceruloplasmin precursor, are described in Table 8 (given according to their position(s) on the amino acid sequence in the first column; the second column indicates whether the glycosylation site is present in the variant protein; and the last column indicates whether the position is different on the variant protein).

TABLE 8 Glycosylation site(s) Position(s) on known Present Position in amino acid sequence in variant protein? variant protein? 138 yes 138 762 yes 762 397 yes 397 358 yes 358

Variant protein HSCP2_PEA_(—)1_P4 (SEQ ID NO: 846) is encoded by the following transcript(s): HSCP2_PEA_(—)1_T4 (SEQ ID NO: 783) and HSCP2_PEA_(—)1_T50 (SEQ ID NO: 794), for which the sequence(s) is/are given at the end of the application.

The coding portion of transcript HSCP2_PEA_(—)1_T4 (SEQ ID NO: 783) is shown in bold; this coding portion starts at position 250 and ends at position 3444. The transcript also has the following SNPs as listed in Table 9 (given according to their position on the nucleotide sequence, with the alternative nucleic acid listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein HSCP2_PEA_(—)1_P4 (SEQ ID NO: 846) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 9 Nucleic acid SNPs SNP position on nucleotide sequence Alternative nucleic acid Previously known SNP? 63 A -> No 201 G -> T No 326 T -> No 335 T -> No 358 T -> C No 360 T -> C No 389 T -> No 409 A -> G No 437 T -> No 524 T -> C No 591 T -> C No 598 T -> A No 692 A -> G No 768 T -> No 807 A -> No 807 A -> G No 818 C -> No 818 C -> G No 837 T -> C No 887 T -> No 901 G -> A No 910 T -> No 952 A -> G No 1006 T -> C No 1053 A -> G Yes 1073 T -> C No 1107 T -> G No 1142 T -> C No 1162 A -> G No 1284 A -> G No 1287 C -> T No 1353 G -> A No 1582 C -> T No 1600 C -> G No 1617 G -> A No 1679 C -> T No 1728 A -> No 1768 T -> C No 1851 T -> C No 1853 T -> C No 1881 T -> A Yes 1938 A -> G No 2000 T -> C No 2042 G -> A Yes 2055 T -> C No 2069 T -> G Yes 2139 T -> C No 2168 A -> G No 2199 A -> C Yes 2228 T -> C No 2274 A -> No 2364 C -> T No 2381 A -> No 2429 T -> C No 2492 A -> No 2525 A -> No 2525 A -> C No 2614 G -> A No 3028 G -> A Yes 3240 T -> C No 3276 A -> G No 3369 C -> G No 5131 C -> A Yes 6091 T -> No 6106 A -> C Yes 6366 G -> A No 6564 G -> A Yes

The coding portion of transcript HSCP2_PEA_(—)1_T50 (SEQ ID NO: 794) is shown in bold; this coding portion starts at position 250 and ends at position 3444. The transcript also has the following SNPs as listed in Table 10 (given according to their position on the nucleotide sequence, with the alternative nucleic acid listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein HSCP2_PEA_(—)1_P4 (SEQ ID NO: 846) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 10 Nucleic acid SNPs SNP position on nucleotide sequence Alternative nucleic acid Previously known SNP? 63 A -> No 201 G -> T No 326 T -> No 335 T -> No 358 T -> C No 360 T -> C No 389 T -> No 409 A -> G No 437 T -> No 524 T -> C No 591 T -> C No 598 T -> A No 692 A -> G No 768 T -> No 807 A -> No 807 A -> G No 818 C -> No 818 C -> G No 837 T -> C No 887 T -> No 901 G -> A No 910 T -> No 952 A -> G No 1006 T -> C No 1053 A -> G Yes 1073 T -> C No 1107 T -> G No 1142 T -> C No 1162 A -> G No 1284 A -> G No 1287 C -> T No 1353 G -> A No 1582 C -> T No 1600 C -> G No 1617 G -> A No 1679 C -> T No 1728 A -> No 1768 T -> C No 1851 T -> C No 1853 T -> C No 1881 T -> A Yes 1938 A -> G No 2000 T -> C No 2042 G -> A Yes 2055 T -> C No 2069 T -> G Yes 2139 T -> C No 2168 A -> G No 2199 A -> C Yes 2228 T -> C No 2274 A -> No 2364 C -> T No 2381 A -> No 2429 T -> C No 2492 A -> No 2525 A -> No 2525 A -> C No 2614 G -> A No 3028 G -> A Yes 3240 T -> C No 3276 A -> G No 3369 C -> G No

Variant protein HSCP2_PEA_(—)1_P8 (SEQ ID NO: 847) according to the present invention has an amino acid sequence as given at the end of the application; it is encoded by transcript(s) HSCP2_PEA_(—)1_T13 (SEQ ID NO: 784). An alignment is given to the known protein (Ceruloplasmin precursor) at the end of the application. One or more alignments to one or more previously published protein sequences are given at the end of the application. A brief description of the relationship of the variant protein according to the present invention to each such aligned protein is as follows:

Comparison report between HSCP2_PEA_(—)1_P8 (SEQ ID NO: 847) and CERU_HUMAN:

1. An isolated chimeric polypeptide encoding for HSCP2_PEA_(—)1_P8 (SEQ ID NO: 847), comprising a first amino acid sequence being at least 90% homologous to MKILILGIFLFLCSTPAWAKEKHYYIGIIETTWDYASDHGEKKLISVDTEHSNIYLQNGPD RIGRLYKKALYLQYTDETFRTTIEKPVWLGFLGPIIKAETGDKVYVHLKNLASRPYTFHS HGITYYKEHEGAIYPDNTTDFQRADDKVYPGEQYTYMLLATEEQSPGEGDGNCVTRIY HSHIDAPKDIASGLIGPLIICKKDSLDKEKEKHIDREFVVMFSVVDENFSWYLEDNIKTY CSEPEKVDKDNEDFQESNRMYSVNGYTFGSLPGLSMCAEDRVKWYLFGMGNEVDVH AAFFHGQALTNKNYRIDTINLFPATLFDAYMVAQNPGEWMLSCQNLNHLKAGLQAFF QVQECNKSSSKDNIRGKHVRHYYIAAEEIIWNYAPSGIDIFTKENLTAPGSDSAVFFEQG TTRIGGSYKKLVYREYTDASFTNRKERGPEEEHLGILGPVIWAEVGDTIRVTFHNKGAY PLSIEPIGVRFNKNNEGTYYSPNYNPQSRSVPPSASHVAPTETFTYEWTVPKEVGPTNAD PVCLAKMYYSAVDPTKDIFTGLIGPMKICKKGSLHANGRQKDVDKEFYLFPTVFDENES LLLEDNIRMFTTAPDQVDKEDEDFQESNKMHSMNGFMYGNQPGLTMCKGDSVVWYL FSAGNEADVHGIYFSGNTYLWRGERRDTANLFPQTSLTLHMWPDTEGTFNVECLTTDH YTGGMKQKYTVNQCRRQSEDSTFYLGERTYYIAAVEVEWDYSPQREWEKELHHLQEQ NVSNAFLDKGEFYIGSKYKKVVYRQYTDSTFRVPVERKAEEEHLGILGPQLHADVGDK VKIIFKNMATRPYSIHAHGVQTESSTVTPTLPGETLTYVWKIPERSGAGTEDSACIPWAY YSTVDQVKDLYSGLIGPLIVCRRPYLKVFNPRRKLEFALLFLVFDENESWYLDDNIKTYS DHPEKVNKDDEEFIESNKMHAINGRMFGNLQGLTMHVGDEVNWYLMGMGNEIDLHT VHFHGHSFQYK corresponding to amino acids 1-1006 of CERU_HUMAN, which also corresponds to amino acids 1-1006 of HSCP2_PEA_(—)1_P8 (SEQ ID NO: 847), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence KCFQEHLEFGYSTAM (SEQ ID NO: 1092) corresponding to amino acids 1007-1021 of HSCP2_PEA_(—)1_P8 (SEQ ID NO: 847), wherein said first amino acid sequence and second amino acid sequence are contiguous and in a sequential order.

2. An isolated polypeptide encoding for a tail of HSCP2_PEA_(—)1_P8 (SEQ ID NO: 847), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence KCFQEHLEFGYSTAM (SEQ ID NO: 1092) in HSCP2_PEA_(—)1_P8 (SEQ ID NO: 847).

The location of the variant protein was determined according to results from a number of different software programs and analyses, including analyses from SignalP and other specialized programs. The variant protein is believed to be located as follows with regard to the cell: secreted. The protein localization is believed to be secreted because both signal-peptide prediction programs predict that this protein has a signal peptide, and neither trans-membrane region prediction program predicts that this protein has a trans-membrane region.

Variant protein HSCP2_PEA_(—)1_P8 (SEQ ID NO: 847) also has the following non-silent SNPs (Single Nucleotide Polymorphisms) as listed in Table 11, (given according to their position(s) on the amino acid sequence, with the alternative amino acid(s) listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein HSCP2_PEA_(—)1_P8 (SEQ ID NO: 847) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 11 Amino acid mutations SNP position(s) on amino acid sequence Alternative amino acid(s) Previously known SNP? 26 I -> No 29 I -> No 37 S -> P No 47 V -> No 54 I -> V No 63 I -> No 92 F -> S No 117 Y -> N No 148 K -> R No 173 N -> No 186 P -> No 190 A -> No 190 A -> G No 213 I -> No 218 V -> M No 221 F -> No 235 N -> D No 253 F -> L No 275 M -> T No 286 F -> L No 298 F -> S No 305 T -> A No 445 H -> Y No 451 P -> A No 477 P -> L No 493 P -> No 507 S -> P No 535 L -> P No 544 D -> E Yes 584 V -> A No 598 R -> K Yes 607 V -> G Yes 640 D -> G No 660 F -> S No 675 A -> No 711 Q -> No 727 F -> S No 748 Q -> No 759 Q -> No 759 Q -> P No 789 D -> N No 927 E -> K Yes 1020 A -> G No

The glycosylation sites of variant protein HSCP2_PEA_(—)1_P8 (SEQ ID NO: 847), as compared to the known protein Ceruloplasmin precursor, are described in Table 12 (given according to their position(s) on the amino acid sequence in the first column; the second column indicates whether the glycosylation site is present in the variant protein; and the last column indicates whether the position is different on the variant protein).

TABLE 12 Glycosylation site(s) Position(s) on known Present Position in amino acid sequence in variant protein? variant protein? 138 yes 138 762 yes 762 397 yes 397 358 yes 358

Variant protein HSCP2_PEA_(—)1_P8 (SEQ ID NO: 847) is encoded by the following transcript(s): HSCP2_PEA_(—)1_T13 (SEQ ID NO: 784), for which the sequence(s) is/are given at the end of the application. The coding portion of transcript HSCP2_PEA_(—)1_T13 (SEQ ID NO: 784) is shown in bold; this coding portion starts at position 250 and ends at position 3312. The transcript also has the following SNPs as listed in Table 13 (given according to their position on the nucleotide sequence, with the alternative nucleic acid listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein HSCP2_PEA_(—)1_P8 (SEQ ID NO: 847) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 13 Nucleic acid SNPs SNP position on nucleotide sequence Alternative nucleic acid Previously known SNP? 63 A -> No 201 G -> T No 326 T -> No 335 T -> No 358 T -> C No 360 T -> C No 389 T -> No 409 A -> G No 437 T -> No 524 T -> C No 591 T -> C No 598 T -> A No 692 A -> G No 768 T -> No 807 A -> No 807 A -> G No 818 C -> No 818 C -> G No 837 T -> C No 887 T -> No 901 G -> A No 910 T -> No 952 A -> G No 1006 T -> C No 1053 A -> G Yes 1073 T -> C No 1107 T -> G No 1142 T -> C No 1162 A -> G No 1284 A -> G No 1287 C -> T No 1353 G -> A No 1582 C -> T No 1600 C -> G No 1617 G -> A No 1679 C -> T No 1728 A -> No 1768 T -> C No 1851 T -> C No 1853 T -> C No 1881 T -> A Yes 1938 A -> G No 2000 T -> C No 2042 G -> A Yes 2055 T -> C No 2069 T -> G Yes 2139 T -> C No 2168 A -> G No 2199 A -> C Yes 2228 T -> C No 2274 A -> No 2364 C -> T No 2381 A -> No 2429 T -> C No 2492 A -> No 2525 A -> No 2525 A -> C No 2614 G -> A No 3028 G -> A Yes 3240 T -> C No 3308 C -> G No 3880 T -> No 3895 A -> C Yes 4155 G -> A No 4353 G -> A Yes

Variant protein HSCP2_PEA_(—)1_P14 (SEQ ID NO: 848) according to the present invention has an amino acid sequence as given at the end of the application; it is encoded by transcript(s) HSCP2_PEA_(—)1_T19 (SEQ ID NO: 785). An alignment is given to the known protein (Ceruloplasmin precursor) at the end of the application. One or more alignments to one or more previously published protein sequences are given at the end of the application. A brief description of the relationship of the variant protein according to the present invention to each such aligned protein is as follows:

Comparison report between HSCP2_PEA_(—)1_P14 (SEQ ID NO: 848) and CERU_HUMAN:

1. An isolated chimeric polypeptide encoding for HSCP2_PEA_(—)1_P14 (SEQ ID NO: 848), comprising a first amino acid sequence being at least 90% homologous to MKILILGIFLFLCSTPAWAKEKHYYIGIIETTWDYASDHGEKKLISVDTEHSNIYLQNGPD RIGRLYKKALYLQYTDETFRTTIEKPVWLGFLGPIIKAETGDKVYVHLKNLASRPYTFHS HGITYYKEHEGAIYPDNTTDFQRADDKVYPGEQYTYMLLATEEQSPGEGDGNCVTRIY HSHIDAPKDIASGLIGPLIICKKDSLDKEKEKHIDREFVVMFSVVDENFSWYLEDNIKTY CSEPEKVDKDNEDFQESNRMYSVNGYTFGSLPGLSMCAEDRVKWYLFGMGNEVDVH AAFFHGQALTNKNYRIDTINLFPATLFDAYMVAQNPGEWMLSCQNLNHLKAGLQAFF QVQECNKSSSKDNIRGKHVRHYYIAAEEIIWNYAPSGIDIFTKENLTAPGSDSAVFFEQG TTRIGGSYKKLVYREYTDASFTNRKERGPEEEHLGILGPVIWAEVGDTIRVTFHNKGAY PLSIEPIGVRFNKNNEGTYYSPNYNPQSRSVPPSASHVAPTETFTYEWTVPKEVGPTNAD PVCLAKMYYSAVDPTKDIFTGLIGPMKICKKGSLHANGRQKDVDKEFYLFPTVFDENES LLLEDNIRMFTTAPDQVDKEDEDFQESNKMH corresponding to amino acids 1-621 of CERU_HUMAN, which also corresponds to amino acids 1-621 of HSCP2_PEA_(—)1_P14 (SEQ ID NO: 848), a second amino acid sequence bridging amino acid sequence comprising of W, and a third amino acid sequence being at least 90% homologous to TFNVECLTTDHYTGGMKQKYTVNQCRRQSEDSTFYLGERTYYIAAVEVEWDYSPQRE WEKELHHLQEQNVSNAFLDKGEFYIGSKYKKVVYRQYTDSTFRVPVERKAEEEHLGIL GPQLHADVGDKVKIIFKNMATRPYSIHAHGVQTESSTVTPTLPGETLTYVWKIPERSGA GTEDSACIPWAYYSTVDQVKDLYSGLIGPLIVCRRPYLKVFNPRRKLEFALLFLVFDENE SWYLDDNIKTYSDHPEKVNKDDEEFIESNKMHAINGRMFGNLQGLTMHVGDEVNWYL MGMGNEIDLHTVHFHGHSFQYKHRGVYSSDVFDIFPGTYQTLEMFPRTPGIWLLHCHV TDHIHAGMETTYTVLQNEDTKSG corresponding to amino acids 694-1065 of CERU_HUMAN, which also corresponds to amino acids 623-994 of HSCP2_PEA_(—)1_P14 (SEQ ID NO: 848), wherein said first amino acid sequence, second amino acid sequence and third amino acid sequence are contiguous and in a sequential order.

2. An isolated polypeptide encoding for an edge portion of HSCP2_PEA_(—)1_P14 (SEQ ID NO: 848), comprising a polypeptide having a length “n”, wherein n is at least about 10 amino acids in length, optionally at least about 20 amino acids in length, preferably at least about 30 amino acids in length, more preferably at least about 40 amino acids in length and most preferably at least about 50 amino acids in length, wherein at least two amino acids comprise HWT having a structure as follows (numbering according to HSCP2_PEA_(—)1_P14 (SEQ ID NO: 848)): a sequence starting from any of amino acid numbers 621-x to 621; and ending at any of amino acid numbers 623+((n−2)−x), in which x varies from 0 to n−2.

The location of the variant protein was determined according to results from a number of different software programs and analyses, including analyses from SignalP and other specialized programs. The variant protein is believed to be located as follows with regard to the cell: secreted. The protein localization is believed to be secreted because both signal-peptide prediction programs predict that this protein has a signal peptide, and neither trans-membrane region prediction program predicts that this protein has a trans-membrane region.

Variant protein HSCP2_PEA_(—)1_P14 (SEQ ID NO: 848) also has the following non-silent SNPs (Single Nucleotide Polymorphisms) as listed in Table 14, (given according to their position(s) on the amino acid sequence, with the alternative amino acid(s) listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein HSCP2_PEA_(—)1_P14 (SEQ ID NO: 848) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 14 Amino acid mutations SNP position(s) on amino acid sequence Alternative amino acid(s) Previously known SNP? 26 I -> No 29 I -> No 37 S -> P No 47 V -> No 54 I -> V No 63 I -> No 92 F -> S No 117 Y -> N No 148 K -> R No 173 N -> No 186 P -> No 190 A -> No 190 A -> G No 213 I -> No 218 V -> M No 221 F -> No 235 N -> D No 253 F -> L No 275 M -> T No 286 F -> L No 298 F -> S No 305 T -> A No 445 H -> Y No 451 P -> A No 477 P -> L No 493 P -> No 507 S -> P No 535 L -> P No 544 D -> E Yes 584 V -> A No 598 R -> K Yes 607 V -> G Yes 640 Q -> No 656 F -> S No 677 Q -> No 688 Q -> No 688 Q -> P No 718 D -> N No 856 E -> K Yes 969 C -> W No

The glycosylation sites of variant protein HSCP2_PEA_(—)1_P14 (SEQ ID NO: 848), as compared to the known protein Ceruloplasmin precursor, are described in Table 15 (given according to their position(s) on the amino acid sequence in the first column; the second column indicates whether the glycosylation site is present in the variant protein; and the last column indicates whether the position is different on the variant protein).

TABLE 15 Glycosylation site(s) Position(s) on known Present Position in amino acid sequence in variant protein? variant protein? 138 yes 138 762 yes 691 397 yes 397 358 yes 358

Variant protein HSCP2_PEA_(—)1_P14 (SEQ ID NO: 848) is encoded by the following transcript(s): HSCP2_PEA_(—)1_T19 (SEQ ID NO: 785), for which the sequence(s) is/are given at the end of the application. The coding portion of transcript HSCP2_PEA_(—)1_T19 (SEQ ID NO: 785) is shown in bold; this coding portion starts at position 250 and ends at position 3231. The transcript also has the following SNPs as listed in Table 16 (given according to their position on the nucleotide sequence, with the alternative nucleic acid listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein HSCP2_PEA_(—)1_P14 (SEQ ID NO: 848) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 16 Nucleic acid SNPs SNP position on nucleotide sequence Alternative nucleic acid Previously known SNP? 63 A -> No 201 G -> T No 326 T -> No 335 T -> No 358 T -> C No 360 T -> C No 389 T -> No 409 A -> G No 437 T -> No 524 T -> C No 591 T -> C No 598 T -> A No 692 A -> G No 768 T -> No 807 A -> No 807 A -> G No 818 C -> No 818 C -> G No 837 T -> C No 887 T -> No 901 G -> A No 910 T -> No 952 A -> G No 1006 T -> C No 1053 A -> G Yes 1073 T -> C No 1107 T -> G No 1142 T -> C No 1162 A -> G No 1284 A -> G No 1287 C -> T No 1353 G -> A No 1582 C -> T No 1600 C -> G No 1617 G -> A No 1679 C -> T No 1728 A -> No 1768 T -> C No 1851 T -> C No 1853 T -> C No 1881 T -> A Yes 1938 A -> G No 2000 T -> C No 2042 G -> A Yes 2055 T -> C No 2069 T -> G Yes 2151 C -> T No 2168 A -> No 2216 T -> C No 2279 A -> No 2312 A -> No 2312 A -> C No 2401 G -> A No 2815 G -> A Yes 3027 T -> C No 3063 A -> G No 3156 C -> G No 3728 T -> No 3743 A -> C Yes 4003 G -> A No 4201 G -> A Yes

Variant protein HSCP2_PEA_(—)1_P15 (SEQ ID NO: 849) according to the present invention has an amino acid sequence as given at the end of the application; it is encoded by transcript(s) HSCP2_PEA_(—)1_T20 (SEQ ID NO: 786). An alignment is given to the known protein (Ceruloplasmin precursor) at the end of the application. One or more alignments to one or more previously published protein sequences are given at the end of the application. A brief description of the relationship of the variant protein according to the present invention to each such aligned protein is as follows:

Comparison report between HSCP2_PEA_(—)1_P15 (SEQ ID NO: 849) and CERU_HUMAN:

1. An isolated chimeric polypeptide encoding for HSCP2_PEA_(—)1_P15 (SEQ ID NO: 849), comprising a first amino acid sequence being at least 90% homologous to MKILILGIFLFLCSTPAWAKEKHYYIGIIETTWDYASDHGEKKLISVDTEHSNIYLQNGPD RIGRLYKKALYLQYTDETFRTTIEKPVWLGFLGPIIKAETGDKVYVHLKNLASRPYTFHS HGITYYKEHEGAIYPDNTTDFQRADDKVYPGEQYTYMLLATEEQSPGEGDGNCVTRIY HSHIDAPKDIASGLIGPLIICKKDSLDKEKEKHIDREFVVMFSVVDENFSWYLEDNIKTY CSEPEKVDKDNEDFQESNRMYSVNGYTFGSLPGLSMCAEDRVKWYLFGMGNEVDVH AAFFHGQALTNKNYRIDTINLFPATLFDAYMVAQNPGEWMLSCQNLNHLKAGLQAFF QVQECNKSSSKDNIRGKHVRHYYIAAEEIIWNYAPSGIDIFTKENLTAPGSDSAVFFEQG TTRIGGSYKKLVYREYTDASFTNRKERGPEEEHLGILGPVIWAEVGDTIRVTFHNKGAY PLSIEPIGVRFNKNNEGTYYSPNYNPQSRSVPPSASHVAPTETFTYEWTVPKEVGPTNAD PVCLAKMYYSAVDPTKDIFTGLIGPMKICKKGSLHANGRQKDVDKEFYLFPTVFDENES LLLEDNIRMFTTAPDQVDKEDEDFQESNKMHSMNGFMYGNQPGLTMCKGDSVVWYL FSAGNEADVHGIYFSGNTYLWRGERRDTANLFPQTSLTLHMWPDTEGTFNVECLTTDH YTGGMKQKYTVNQCRRQSEDSTFYLGERTYYIAAVEVEWDYSPQREWEKELHHLQEQ NVSNAFLDKGEFYIGSKYKKVVYRQYTDSTFRVPVERKAEEEHLGILGPQLHADVGDK VKIIFKNMATRPYSIHAHGVQTESSTVTPTLPGETLTYVWKIPERSGAGTEDSACIPWAY YSTVDQVKDLYSGLIGPLIVCRRPYLKVFNPRRKLEFALLFLVFDENESWYLDDNIKTYS DHPEKVNKDDEEFIESNKMHAINGRMFGNLQGLTMHVGDEVNWYLMGMGNEIDLHT VHFHGHSFQYKHRGVYSSDVFDIFPGTYQTLEMFPRTPGIWLLHCHVTDHIHAGMETT YTVLQNE corresponding to amino acids 1-1060 of CERU_HUMAN, which also corresponds to amino acids 1-1060 of HSCP2_PEA_(—)1_P15 (SEQ ID NO: 849), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence GEYPASSETHRRIWNVIYPITVSVIILFQISTKE (SEQ ID NO: 1093) corresponding to amino acids 1061-1094 of HSCP2_PEA_(—)1_P15 (SEQ ID NO: 849), wherein said first amino acid sequence and second amino acid sequence are contiguous and in a sequential order.

2. An isolated polypeptide encoding for a tail of HSCP2_PEA_(—)1_P15 (SEQ ID NO: 849), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence GEYPASSETHRRIWNVIYPITVSVIILFQISTKE (SEQ ID NO: 1093) in HSCP2_PEA_(—)1_P15 (SEQ ID NO: 849).

The location of the variant protein was determined according to results from a number of different software programs and analyses, including analyses from SignalP and other specialized programs. The variant protein is believed to be located as follows with regard to the cell: secreted. The protein localization is believed to be secreted because both signal-peptide prediction programs predict that this protein has a signal peptide, and neither trans-membrane region prediction program predicts that this protein has a trans-membrane region.

Variant protein HSCP2_PEA_(—)1_P15 (SEQ ID NO: 849) also has the following non-silent SNPs (Single Nucleotide Polymorphisms) as listed in Table 17, (given according to their position(s) on the amino acid sequence, with the alternative amino acid(s) listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein HSCP2_PEA_(—)1_P15 (SEQ ID NO: 849) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 17 Amino acid mutations SNP position(s) on amino acid sequence Alternative amino acid(s) Previously known SNP? 26 I -> No 29 I -> No 37 S -> P No 47 V -> No 54 I -> V No 63 I -> No 92 F -> S No 117 Y -> N No 148 K -> R No 173 N -> No 186 P -> No 190 A -> No 190 A -> G No 213 I -> No 218 V -> M No 221 F -> No 235 N -> D No 253 F -> L No 275 M -> T No 286 F -> L No 298 F -> S No 305 T -> A No 445 H -> Y No 451 P -> A No 477 P -> L No 493 P -> No 507 S -> P No 535 L -> P No 544 D -> E Yes 584 V -> A No 598 R -> K Yes 607 V -> G Yes 640 D -> G No 660 F -> S No 675 A -> No 711 Q -> No 727 F -> S No 748 Q -> No 759 Q -> No 759 Q -> P No 789 D -> N No 927 E -> K Yes 1040 C -> W No

The glycosylation sites of variant protein HSCP2_PEA_(—)1_P15 (SEQ ID NO: 849), as compared to the known protein Ceruloplasmin precursor, are described in Table 18 (given according to their position(s) on the amino acid sequence in the first column; the second column indicates whether the glycosylation site is present in the variant protein; and the last column indicates whether the position is different on the variant protein).

TABLE 18 Glycosylation site(s) Position(s) on known Present Position in amino acid sequence in variant protein? variant protein? 138 yes 138 762 yes 762 397 yes 397 358 yes 358

Variant protein HSCP2_PEA_(—)1_P15 (SEQ ID NO: 849) is encoded by the following transcript(s): HSCP2_PEA_(—)1_T20 (SEQ ID NO: 786), for which the sequence(s) is/are given at the end of the application. The coding portion of transcript HSCP2_PEA_(—)1_T20 (SEQ ID NO: 786) is shown in bold; this coding portion starts at position 250 and ends at position 3531. The transcript also has the following SNPs as listed in Table 19 (given according to their position on the nucleotide sequence, with the alternative nucleic acid listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein HSCP2_PEA_(—)1_P15 (SEQ ID NO: 849) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 19 Nucleic acid SNPs SNP position on nucleotide sequence Alternative nucleic acid Previously known SNP? 63 A -> No 201 G -> T No 326 T -> No 335 T -> No 358 T -> C No 360 T -> C No 389 T -> No 409 A -> G No 437 T -> No 524 T -> C No 591 T -> C No 598 T -> A No 692 A -> G No 768 T -> No 807 A -> No 807 A -> G No 818 C -> No 818 C -> G No 837 T -> C No 887 T -> No 901 G -> A No 910 T -> No 952 A -> G No 1006 T -> C No 1053 A -> G Yes 1073 T -> C No 1107 T -> G No 1142 T -> C No 1162 A -> G No 1284 A -> G No 1287 C -> T No 1353 G -> A No 1582 C -> T No 1600 C -> G No 1617 G -> A No 1679 C -> T No 1728 A -> No 1768 T -> C No 1851 T -> C No 1853 T -> C No 1881 T -> A Yes 1938 A -> G No 2000 T -> C No 2042 G -> A Yes 2055 T -> C No 2069 T -> G Yes 2139 T -> C No 2168 A -> G No 2199 A -> C Yes 2228 T -> C No 2274 A -> No 2364 C -> T No 2381 A -> No 2429 T -> C No 2492 A -> No 2525 A -> No 2525 A -> C No 2614 G -> A No 3028 G -> A Yes 3240 T -> C No 3276 A -> G No 3369 C -> G No 3623 T -> Yes 3828 G -> T No 3978 T -> No 3979 C -> No

Variant protein HSCP2_PEA_(—)1_P2 (SEQ ID NO: 850) according to the present invention has an amino acid sequence as given at the end of the application; it is encoded by transcript(s) HSCP2_PEA_(—)1_T22 (SEQ ID NO: 787). An alignment is given to the known protein (Ceruloplasmin precursor) at the end of the application. One or more alignments to one or more previously published protein sequences are given at the end of the application. A brief description of the relationship of the variant protein according to the present invention to each such aligned protein is as follows:

Comparison report between HSCP2_PEA_(—)1_P2 (SEQ ID NO: 850) and CERU_HUMAN:

1. An isolated chimeric polypeptide encoding for HSCP2_PEA_(—)1_P2 (SEQ ID NO: 850), comprising a first amino acid sequence being at least 90% homologous to MKILILGIFLFLCSTPAWAKEKHYYIGIIETTWDYASDHGEKKLISVDTEHSNIYLQNGPD RIGRLYKKALYLQYTDETFRTTIEKPVWLGFLGPIIKAETGDKVYVHLKNLASRPYTFHS HGITYYKEHEGAIYPDNTTDFQRADDKVYPGEQYTYMLLATEEQSPGEGDGNCVTRIY HSHIDAPKDIASGLIGPLIICKKDSLDKEKEKHIDREFVVMFSVVDENFSWYLEDNIKTY CSEPEKVDKDNEDFQESNRMYSVNGYTFGSLPGLSMCAEDRVKWYLFGMGNEVDVH AAFFHGQALTNKNYRIDTINLFPATLFDAYMVAQNPGEWMLSCQNLNHLKAGLQAFF QVQECNKSSSKDNIRGKHVRHYYIAAEEIIWNYAPSGIDIFTKENLTAPGSDSAVFFEQG TTRIGGSYKKLVYREYTDASFTNRKERGPEEEHLGILGPVIWAEVGDTIRVTFHNKGAY PLSIEPIGVRFNKNNEGTYYSPNYNPQSRSVPPSASHVAPTETFTYEWTVPKEVGPTNAD PVCLAKMYYSAVDPTKDIFTGLIGPMKICKKGSLHANGRQKDVDKEFYLFPTVFDENES LLLEDNIRMFTTAPDQVDKEDEDFQESNKMHSMNGFMYGNQPGLTMCKGDSVVWYL FSAGNEADVHGIYFSGNTYLWRGERRDTANLFPQTSLTLHMWPDTEGTFNVECLTTDH YTGGMKQKYTVNQCRRQSEDSTFYLGERTYYIAAVEVEWDYSPQREWEKELHHLQEQ corresponding to amino acids 1-761 of CERU_HUMAN, which also corresponds to amino acids 1-761 of HSCP2_PEA_(—)1_P2 (SEQ ID NO: 850), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence K corresponding to amino acids 762-762 of HSCP2_PEA_(—)1_P2 (SEQ ID NO: 850), wherein said first amino acid sequence and second amino acid sequence are contiguous and in a sequential order.

The location of the variant protein was determined according to results from a number of different software programs and analyses, including analyses from SignalP and other specialized programs. The variant protein is believed to be located as follows with regard to the cell: secreted. The protein localization is believed to be secreted because both signal-peptide prediction programs predict that this protein has a signal peptide, and neither trans-membrane region prediction program predicts that this protein has a trans-membrane region.

Variant protein HSCP2_PEA_(—)1_P2 (SEQ ID NO: 850) also has the following non-silent SNPs (Single Nucleotide Polymorphisms) as listed in Table 20, (given according to their position(s) on the amino acid sequence, with the alternative amino acid(s) listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein HSCP2_PEA_(—)1_P2 (SEQ ID NO: 850) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 20 Amino acid mutations SNP position(s) on amino acid sequence Alternative amino acid(s) Previously known SNP? 26 I -> No 29 I -> No 37 S -> P No 47 V -> No 54 I -> V No 63 I -> No 92 F -> S No 117 Y -> N No 148 K -> R No 173 N -> No 186 P -> No 190 A -> No 190 A -> G No 213 I -> No 218 V -> M No 221 F -> No 235 N -> D No 253 F -> L No 275 M -> T No 286 F -> L No 298 F -> S No 305 T -> A No 445 H -> Y No 451 P -> A No 477 P -> L No 493 P -> No 507 S -> P No 535 L -> P No 544 D -> E Yes 584 V -> A No 598 R -> K Yes 607 V -> G Yes 640 D -> G No 660 F -> S No 675 A -> No 711 Q -> No 727 F -> S No 748 Q -> No 759 Q -> No 759 Q -> P No

The glycosylation sites of variant protein HSCP2_PEA_(—)1_P2 (SEQ ID NO: 850), as compared to the known protein Ceruloplasmin precursor, are described in Table 21 (given according to their position(s) on the amino acid sequence in the first column; the second column indicates whether the glycosylation site is present in the variant protein; and the last column indicates whether the position is different on the variant protein).

TABLE 21 Glycosylation site(s) Position(s) on known Present Position in amino acid sequence in variant protein? variant protein? 138 yes 138 762 no 397 yes 397 358 yes 358

Variant protein HSCP2_PEA_(—)1_P2 (SEQ ID NO: 850) is encoded by the following transcript(s): HSCP2_PEA_(—)1_T22 (SEQ ID NO: 787), for which the sequence(s) is/are given at the end of the application. The coding portion of transcript HSCP2_PEA_(—)1_T22 (SEQ ID NO: 787) is shown in bold; this coding portion starts at position 250 and ends at position 2535. The transcript also has the following SNPs as listed in Table 22 (given according to their position on the nucleotide sequence, with the alternative nucleic acid listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein HSCP2_PEA_(—)1_P2 (SEQ ID NO: 850) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 22 Nucleic acid SNPs SNP position on nucleotide sequence Alternative nucleic acid Previously known SNP? 63 A -> No 201 G -> T No 326 T -> No 335 T -> No 358 T -> C No 360 T -> C No 389 T -> No 409 A -> G No 437 T -> No 524 T -> C No 591 T -> C No 598 T -> A No 692 A -> G No 768 T -> No 807 A -> No 807 A -> G No 818 C -> No 818 C -> G No 837 T -> C No 887 T -> No 901 G -> A No 910 T -> No 952 A -> G No 1006 T -> C No 1053 A -> G Yes 1073 T -> C No 1107 T -> G No 1142 T -> C No 1162 A -> G No 1284 A -> G No 1287 C -> T No 1353 G -> A No 1582 C -> T No 1600 C -> G No 1617 G -> A No 1679 C -> T No 1728 A -> No 1768 T -> C No 1851 T -> C No 1853 T -> C No 1881 T -> A Yes 1938 A -> G No 2000 T -> C No 2042 G -> A Yes 2055 T -> C No 2069 T -> G Yes 2139 T -> C No 2168 A -> G No 2199 A -> C Yes 2228 T -> C No 2274 A -> No 2364 C -> T No 2381 A -> No 2429 T -> C No 2492 A -> No 2525 A -> No 2525 A -> C No 2565 A -> No 2676 G -> A No 3195 T -> A Yes 3482 G -> A Yes 3542 A -> G No 3975 G -> A No

Variant protein HSCP2_PEA_(—)1_P16 (SEQ ID NO: 851) according to the present invention has an amino acid sequence as given at the end of the application; it is encoded by transcript(s) HSCP2_PEA_(—)1_T23 (SEQ ID NO: 788). An alignment is given to the known protein (Ceruloplasmin precursor) at the end of the application. One or more alignments to one or more previously published protein sequences are given at the end of the application. A brief description of the relationship of the variant protein according to the present invention to each such aligned protein is as follows:

Comparison report between HSCP2_PEA_(—)1_P16 (SEQ ID NO: 851) and CERU_HUMAN:

1. An isolated chimeric polypeptide encoding for HSCP2_PEA_(—)1_P16 (SEQ ID NO: 851), comprising a first amino acid sequence being at least 90% homologous to MKILILGIFLFLCSTPAWAKEKHYYIGIIETTWDYASDHGEKKLISVDTEHSNIYLQNGPD RIGRLYKKALYLQYTDETFRTTIEKPVWLGFLGPIIKAETGDKVYVHLKNLASRPYTFHS HGITYYKEHEGAIYPDNTTDFQRADDKVYPGEQYTYMLLATEEQSPGEGDGNCVTRIY HSHIDAPKDIASGLIGPLIICKKDSLDKEKEKHIDREFVVMFSVVDENFSWYLEDNIKTY CSEPEKVDKDNEDFQESNRMYSVNGYTFGSLPGLSMCAEDRVKWYLFGMGNEVDVH AAFFHGQALTNKNYRIDTINLFPATLFDAYMVAQNPGEWMLSCQNLNHLKAGLQAFF QVQECNKSSSKDNIRGKHVRHYYIAAEEIIWNYAPSGIDIFTKENLTAPGSDSAVFFEQG TTRIGGSYKKLVYREYTDASFTNRKERGPEEEHLGILGPVIWAEVGDTIRVTFHNKGAY PLSIEPIGVRFNKNNEGTYYSPNYNPQSRSVPPSASHVAPTETFTYEWTVPKEVGPTNAD PVCLAKMYYSAVDPTKDIFTGLIGPMKICKKGSLHANGRQKDVDKEFYLFPTVFDENES LLLEDNIRMFTTAPDQVDKEDEDFQESNKMHSMNGFMYGNQPGLTMCKGDSVVWYL FSAGNEADVHGIYFSGNTYLWRGERRDTANLFPQTSLTLHMWPDTEGTFNVECLTTDH YTGGMKQKYTVNQCRRQSEDSTFYLGERTYYIAAVEVEWDYSPQREWEKELHHLQEQ NVSNAFLDKGEFYIGSKYKKVVYRQYTDSTFRVPVERKAEEEHLGILGPQLHADVGDK VKIIFKNMATRPYSIHAHGVQTESSTVTPTLPGETLTYVWKIPERSGAGTEDSACIPWAY YSTVDQVKDLYSGLIGPLIVCRRPYLKVFNPRRKLEFALLFLVFDENESWYLDDNIKTYS DHPEKVNKDDEEFIESNKMHAINGRMFGNLQGLTMHVGDEVNWYLMGMGNEIDLHT VHFHGHSFQYKH corresponding to amino acids 1-1007 of CERU_HUMAN, which also corresponds to amino acids 1-1007 of HSCP2_PEA_(—)1_P16 (SEQ ID NO: 851), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence LLRLTGEYGM (SEQ ID NO: 1094) corresponding to amino acids 1008-1017 of HSCP2_PEA_(—)1_P16 (SEQ ID NO: 851), wherein said first amino acid sequence and second amino acid sequence are contiguous and in a sequential order.

2. An isolated polypeptide encoding for a tail of HSCP2_PEA_(—)1_P16 (SEQ ID NO: 851), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence LLRLTGEYGM (SEQ ID NO: 1094) in HSCP2_PEA_(—)1_P16 (SEQ ID NO: 851).

The location of the variant protein was determined according to results from a number of different software programs and analyses, including analyses from SignalP and other specialized programs. The variant protein is believed to be located as follows with regard to the cell: secreted. The protein localization is believed to be secreted because both signal-peptide prediction programs predict that this protein has a signal peptide, and neither trans-membrane region prediction program predicts that this protein has a trans-membrane region.

Variant protein HSCP2_PEA_(—)1_P16 (SEQ ID NO: 851) also has the following non-silent SNPs (Single Nucleotide Polymorphisms) as listed in Table 23 (given according to their position(s) on the amino acid sequence, with the alternative amino acid(s) listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein HSCP2_PEA_(—)1_P16 (SEQ ID NO: 851) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 23 Amino acid mutations SNP position(s) on amino acid sequence Alternative amino acid(s) Previously known SNP? 26 I -> No 29 I -> No 37 S -> P No 47 V -> No 54 I -> V No 63 I -> No 92 F -> S No 117 Y -> N No 148 K -> R No 173 N -> No 186 P -> No 190 A -> G No 190 A -> No 213 I -> No 218 V -> M No 221 F -> No 235 N -> D No 253 F -> L No 275 M -> T No 286 F -> L No 298 F -> S No 305 T -> A No 445 H -> Y No 451 P -> A No 477 P -> L No 493 P -> No 507 S -> P No 535 L -> P No 544 D -> E Yes 584 V -> A No 598 R -> K Yes 607 V -> G Yes 640 D -> G No 660 F -> S No 675 A -> No 711 Q -> No 727 F -> S No 748 Q -> No 759 Q -> No 759 Q -> P No 789 D -> N No 927 E -> K Yes

The glycosylation sites of variant protein HSCP2_PEA_(—)1_P16 (SEQ ID NO: 851), as compared to the known protein Ceruloplasmin precursor, are described in Table 24 (given according to their position(s) on the amino acid sequence in the first column; the second column indicates whether the glycosylation site is present in the variant protein; and the last column indicates whether the position is different on the variant protein).

TABLE 24 Glycosylation site(s) Position(s) on known Present Position in amino acid sequence in variant protein? variant protein? 138 yes 138 762 yes 762 397 yes 397 358 yes 358

Variant protein HSCP2_PEA_(—)1_P16 (SEQ ID NO: 851) is encoded by the following transcript(s): HSCP2_PEA_(—)1_T23 (SEQ ID NO: 788), for which the sequence(s) is/are given at the end of the application. The coding portion of transcript HSCP2_PEA_(—)1_T23 (SEQ ID NO: 788) is shown in bold; this coding portion starts at position 250 and ends at position 3300. The transcript also has the following SNPs as listed in Table 25 (given according to their position on the nucleotide sequence, with the alternative nucleic acid listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein HSCP2_PEA_(—)1_P16 (SEQ ID NO: 851) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 25 Nucleic acid SNPs SNP position on nucleotide sequence Alternative nucleic acid Previously known SNP? 63 A -> No 201 G -> T No 326 T -> No 335 T -> No 358 T -> C No 360 T -> C No 389 T-> No 409 A -> G No 437 T -> No 524 T -> C No 591 T -> C No 598 T -> A No 692 A -> G No 768 T -> No 807 A -> No 807 A -> G No 818 C -> No 818 C -> G No 837 T -> C No 887 T -> No 901 G -> A No 910 T -> No 952 A -> G No 1006 T -> C No 1053 A -> G Yes 1073 T -> C No 1107 T -> G No 1142 T -> C No 1162 A -> G No 1284 A -> G No 1287 C -> T No 1353 G -> A No 1582 C -> T No 1600 C -> G No 1617 G -> A No 1679 C -> T No 1728 A -> No 1768 T -> C No 1851 T -> C No 1853 T -> C No 1881 T -> A Yes 1938 A -> G No 2000 T -> C No 2042 G -> A Yes 2055 T -> C No 2069 T -> G Yes 2139 T -> C No 2168 A -> G No 2199 A -> C Yes 2228 T -> C No 2274 A -> No 2364 C -> T No 2381 A -> No 2429 T -> C No 2492 A -> No 2525 A -> No 2525 A -> C No 2614 G -> A No 3028 G -> A Yes 3240 T -> C No 3448 T -> Yes 3653 G -> T No 3803 T -> No 3804 C -> No

Variant protein HSCP2_PEA_(—)1_P6 (SEQ ID NO: 852) according to the present invention has an amino acid sequence as given at the end of the application; it is encoded by transcript(s) HSCP2_PEA_(—)1_T25 (SEQ ID NO: 789). An alignment is given to the known protein (Ceruloplasmin precursor) at the end of the application. One or more alignments to one or more previously published protein sequences are given at the end of the application. A brief description of the relationship of the variant protein according to the present invention to each such aligned protein is as follows:

Comparison report between HSCP2_PEA_(—)1_P6 (SEQ ID NO: 852) and CERU_HUMAN:

1. An isolated chimeric polypeptide encoding for HSCP2_PEA_(—)1_P6 (SEQ ID NO: 852), comprising a first amino acid sequence being at least 90% homologous to MKILILGIFLFLCSTPAWAKEKHYYIGIIETTWDYASDHGEKKLISVDTEHSNIYLQNGPD RIGRLYKKALYLQYTDETFRTTIEKPVWLGFLGPIIKAETGDKVYVHLKNLASRPYTFHS HGITYYKEHEGAIYPDNTTDFQRADDKVYPGEQYTYMLLATEEQSPGEGDGNCVTRIY HSHIDAPKDIASGLIGPLIICKKDSLDKEKEKHIDREFVVMFSVVDENFSWYLEDNIKTY CSEPEKVDKDNEDFQESNRMYSVNGYTFGSLPGLSMCAEDRVKWYLFGMGNEVDVH AAFFHGQALTNKNYRIDTINLFPATLFDAYMVAQNPGEWMLSCQNLNHLKAGLQAFF QVQECNKSSSKDNIRGKHVRHYYIAAEEIIWNYAPSGIDIFTKENLTAPGSDSAVFFEQG TTRIGGSYKKLVYREYTDASFTNRKERGPEEEHLGILGPVIWAEVGDTIRVTFHNKGAY PLSIEPIGVRFNKNNEGTYYSPNYNPQSRSVPPSASHVAPTETFTYEWTVPKEVGPTNAD PVCLAKMYYSAVDPTKDIFTGLIGPMKICKKGSLHANGRQKDVDKEFYLFPTVFDENES LLLEDNIRMFTTAPDQVDKEDEDFQESNKMHSMNGFMYGNQPGLTMCKGDSVVWYL FSAGNEADVHGIYFSGNTYLWRGERRDTANLFPQTSLTLHMWPDTEGTFNVECLTTDH YTGGMKQKYTVNQCRRQSEDSTFYLGERTYYIAAVEVEWDYSPQREWEKELHHLQEQ NVSNAFLDKGEFYIGSKYKKVVYRQYTDSTFRVPVERKAEEEHLGILGPQLHADVGDK VKIIFKNMATRPYSIHAHGVQTESSTVTPTLPGETLTYVWKIPERSGAGTEDSACIPWAY YSTVDQVKDLYSGLIGPLIVCRRPYLKVFNPRRKLEFALLFLVFDENESWYLDDNIKTYS DHPEKVNKDDEEFIESNKMHAINGRMFGNLQGLTMHVGDEVNWYLMGMGNEIDLHT VHFHGHSFQYK corresponding to amino acids 1-1006 of CERU_HUMAN, which also corresponds to amino acids 1-1006 of HSCP2_PEA_(—)1_P6 (SEQ ID NO: 852), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence GSL corresponding to amino acids 1007-1009 of HSCP2_PEA_(—)1_P6 (SEQ ID NO: 852), wherein said first amino acid sequence and second amino acid sequence are contiguous and in a sequential order.

The location of the variant protein was determined according to results from a number of different software programs and analyses, including analyses from SignalP and other specialized programs. The variant protein is believed to be located as follows with regard to the cell: secreted. The protein localization is believed to be secreted because both signal-peptide prediction programs predict that this protein has a signal peptide, and neither trans-membrane region prediction program predicts that this protein has a trans-membrane region.

Variant protein HSCP2_PEA_(—)1_P6 (SEQ ID NO: 852) also has the following non-silent SNPs (Single Nucleotide Polymorphisms) as listed in Table 26, (given according to their position(s) on the amino acid sequence, with the alternative amino acid(s) listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein HSCP2_PEA_(—)1_P6 (SEQ ID NO: 852) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 26 Amino acid mutations SNP position(s) on amino acid sequence Alternative amino acid(s) Previously known SNP? 26 I -> No 29 I -> No 37 S -> P No 47 V -> No 54 I -> V No 63 I -> No 92 F -> S No 117 Y -> N No 148 K -> R No 173 N -> No 186 P -> No 190 A -> No 190 A -> G No 213 I -> No 218 V -> M No 221 F -> No 235 N -> D No 253 F -> L No 275 M -> T No 286 F -> L No 298 F -> S No 305 T -> A No 445 H -> Y No 451 P -> A No 477 P -> L No 493 P -> No 507 S -> P No 535 L -> P No 544 D -> E Yes 584 V -> A No 598 R -> K Yes 607 V -> G Yes 640 D -> G No 660 F -> S No 675 A -> No 711 Q -> No 727 F -> S No 748 Q -> No 759 Q -> No 759 Q -> P No 789 D -> N No 927 E -> K Yes 1008 S -> G No

The glycosylation sites of variant protein HSCP2_PEA_(—)1_P6 (SEQ ID NO: 852), as compared to the known protein Ceruloplasmin precursor, are described in Table 27 (given according to their position(s) on the amino acid sequence in the first column; the second column indicates whether the glycosylation site is present in the variant protein; and the last column indicates whether the position is different on the variant protein).

TABLE 27 Glycosylation site(s) Position(s) on known Present Position in amino acid sequence in variant protein? variant protein? 138 yes 138 762 yes 762 397 yes 397 358 yes 358

Variant protein HSCP2_PEA_(—)1_P6 (SEQ ID NO: 852) is encoded by the following transcript(s): HSCP2_PEA_(—)1_T25 (SEQ ID NO: 789), for which the sequence(s) is/are given at the end of the application. The coding portion of transcript HSCP2_PEA_(—)1_T25 (SEQ ID NO: 789) is shown in bold; this coding portion starts at position 250 and ends at position 3276. The transcript also has the following SNPs as listed in Table 28 (given according to their position on the nucleotide sequence, with the alternative nucleic acid listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein HSCP2_PEA_(—)1_P6 (SEQ ID NO: 852) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 28 Nucleic acid SNPs SNP position on nucleotide sequence Alternative nucleic acid Previously known SNP? 63 A -> No 201 G -> T No 326 T -> No 335 T -> No 358 T -> C No 360 T -> C No 389 T -> No 409 A -> G No 437 T -> No 524 T -> C No 591 T -> C No 598 T -> A No 692 A -> G No 768 T -> No 807 A -> No 807 A -> G No 818 C -> No 818 C -> G No 837 T -> C No 887 T -> No 901 G -> A No 910 T -> No 952 A -> G No 1006 T -> C No 1053 A -> G Yes 1073 T -> C No 1107 T -> G No 1142 T -> C No 1162 A -> G No 1284 A -> G No 1287 C -> T No 1353 G -> A No 1582 C -> T No 1600 C -> G No 1617 G -> A No 1679 C -> T No 1728 A -> No 1768 T -> C No 1851 T -> C No 1853 T -> C No 1881 T -> A Yes 1938 A -> G No 2000 T -> C No 2042 G -> A Yes 2055 T -> C No 2069 T -> G Yes 2139 T -> C No 2168 A -> G No 2199 A -> C Yes 2228 T -> C No 2274 A -> No 2364 C -> T No 2381 A -> No 2429 T -> C No 2492 A -> No 2525 A -> No 2525 A -> C No 2614 G -> A No 3028 G -> A Yes 3240 T -> C No 3271 A -> G No 3364 C -> G No

Variant protein HSCP2_PEA_(—)1_P2 (SEQ ID NO: 850)2 according to the present invention has an amino acid sequence as given at the end of the application; it is encoded by transcript(s) HSCP2_PEA_(—)1_T31 (SEQ ID NO: 790). An alignment is given to the known protein (Ceruloplasmin precursor) at the end of the application. One or more alignments to one or more previously published protein sequences are given at the end of the application. A brief description of the relationship of the variant protein according to the present invention to each such aligned protein is as follows:

Comparison report between HSCP2_PEA_(—)1_P2 (SEQ ID NO: 850)2 and CERU_HUMAN:

1. An isolated chimeric polypeptide encoding for HSCP2_PEA_(—)1_P2 (SEQ ID NO: 850)2, comprising a first amino acid sequence being at least 90% homologous to MKILILGIFLFLCSTPAWAKEKHYYIGIIETTWDYASDHGEKKLISVDTEHSNIYLQNGPD RIGRLYKKALYLQYTDETFRTTIEKPVWLGFLGPIIKAETGDKVYVHLKNLASRPYTFHS HGITYYKEHE corresponding to amino acids 1-131 of CERU_HUMAN, which also corresponds to amino acids 1-131 of HSCP2_PEA_(—)1_P22 (SEQ ID NO: 853), a second amino acid sequence bridging amino acid sequence comprising of A, and a third amino acid sequence being at least 90% homologous to VNGYTFGSLPGLSMCAEDRVKWYLFGMGNEVDVHAAFFHGQALTNKNYRIDTINLFP ATLFDAYMVAQNPGEWMLSCQNLNHLKAGLQAFFQVQECNKSSSKDNIRGKHVRHY YIAAEEIIWNYAPSGIDIFTKENLTAPGSDSAVFFEQGTTRIGGSYKKLVYREYTDASFTN RKERGPEEEHLGILGPVIWAEVGDTIRVTFHNKGAYPLSIEPIGVRFNKNNEGTYYSPNY NPQSRSVPPSASHVAPTETFTYEWTVPKEVGPTNADPVCLAKMYYSAVDPTKDIFTGLI GPMKICKKGSLHANGRQKDVDKEFYLFPTVFDENESLLLEDNIRMFTTAPDQVDKEDE DFQESNKMHSMNGFMYGNQPGLTMCKGDSVVWYLFSAGNEADVHGIYFSGNTYLWR GERRDTANLFPQTSLTLHMWPDTEGTFNVECLTTDHYTGGMKQKYTVNQCRRQSEDS TFYLGERTYYIAAVEVEWDYSPQREWEKELHHLQEQNVSNAFLDKGEFYIGSKYKKVV YRQYTDSTFRVPVERKAEEEHLGILGPQLHADVGDKVKIIFKNMATRPYSIHAHGVQTE SSTVTPTLPGETLTYVWKIPERSGAGTEDSACIPWAYYSTVDQVKDLYSGLIGPLIVCRR PYLKVFNPRRKLEFALLFLVFDENESWYLDDNIKTYSDHPEKVNKDDEEFIESNKMHAI NGRMFGNLQGLTMHVGDEVNWYLMGMGNEIDLHTVHFHGHSFQYKHRGVYSSDVF DIFPGTYQTLEMFPRTPGIWLLHCHVTDHIHAGMETTYTVLQNEDTKSG corresponding to amino acids 262-1065 of CERU_HUMAN, which also corresponds to amino acids 133-936 of HSCP2_PEA_(—)1_P22 (SEQ ID NO: 853), wherein said first amino acid sequence, second amino acid sequence and third amino acid sequence are contiguous and in a sequential order.

2. An isolated polypeptide encoding for an edge portion of HSCP2_PEA_(—)1_P22 (SEQ ID NO: 853), comprising a polypeptide having a length “n”, wherein n is at least about 10 amino acids in length, optionally at least about 20 amino acids in length, preferably at least about 30 amino acids in length, more preferably at least about 40 amino acids in length and most preferably at least about 50 amino acids in length, wherein at least two amino acids comprise EAV having a structure as follows (numbering according to HSCP2_PEA_(—)1_P22 (SEQ ID NO: 853)): a sequence starting from any of amino acid numbers 131-x to 131; and ending at any of amino acid numbers 133+((n−2)−x), in which x varies from 0 to n−2.

The location of the variant protein was determined according to results from a number of different software programs and analyses, including analyses from SignalP and other specialized programs. The variant protein is believed to be located as follows with regard to the cell: secreted. The protein localization is believed to be secreted because both signal-peptide prediction programs predict that this protein has a signal peptide, and neither trans-membrane region prediction program predicts that this protein has a trans-membrane region.

Variant protein HSCP2_PEA_(—)1_P22 (SEQ ID NO: 853) also has the following non-silent SNPs (Single Nucleotide Polymorphisms) as listed in Table 29, (given according to their position(s) on the amino acid sequence, with the alternative amino acid(s) listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein HSCP2_PEA_(—)1_P22 (SEQ ID NO: 853) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 29 Amino acid mutations SNP position(s) on amino acid sequence Alternative amino acid(s) Previously known SNP? 26 I -> No 29 I -> No 37 S -> P No 47 V -> No 54 I -> V No 63 I -> No 92 F -> S No 117 Y -> N No 146 M -> T No 157 F -> L No 169 F -> S No 176 T -> A No 316 H -> Y No 322 P -> A No 348 P -> L No 364 P -> No 378 S -> P No 406 L -> P No 415 D -> E Yes 455 V -> A No 469 R -> K Yes 478 V -> G Yes 511 D -> G No 531 F -> S No 546 A -> No 582 Q -> No 598 F -> S No 619 Q -> No 630 Q -> P No 630 Q -> No 660 D -> N No 798 E -> K Yes 911 C -> W No

The glycosylation sites of variant protein HSCP2_PEA_(—)1_P22 (SEQ ID NO: 853), as compared to the known protein Ceruloplasmin precursor, are described in Table 30 (given according to their position(s) on the amino acid sequence in the first column; the second column indicates whether the glycosylation site is present in the variant protein; and the last column indicates whether the position is different on the variant protein).

TABLE 30 Glycosylation site(s) Position(s) on known Present Position in amino acid sequence in variant protein? variant protein? 138 no 762 yes 633 397 yes 268 358 yes 229

Variant protein HSCP2_PEA_(—)1_P22 (SEQ ID NO: 853) is encoded by the following transcript(s): HSCP2_PEA_(—)1_T31 (SEQ ID NO: 790), for which the sequence(s) is/are given at the end of the application. The coding portion of transcript HSCP2_PEA_(—)1_T31 (SEQ ID NO: 790) is shown in bold; this coding portion starts at position 250 and ends at position 3057. The transcript also has the following SNPs as listed in Table 31 (given according to their position on the nucleotide sequence, with the alternative nucleic acid listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein HSCP2_PEA_(—)1_P22 (SEQ ID NO: 853) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 31 Nucleic acid SNPs SNP position on nucleotide sequence Alternative nucleic acid Previously known SNP? 63 A -> No 201 G -> T No 326 T -> No 335 T -> No 358 T -> C No 360 T -> C No 389 T -> No 409 A -> G No 437 T -> No 524 T -> C No 591 T -> C No 598 T -> A No 666 A -> G Yes 686 T -> C No 720 T -> G No 755 T -> C No 775 A -> G No 897 A -> G No 900 C -> T No 966 G -> A No 1195 C -> T No 1213 C -> G No 1230 G -> A No 1292 C -> T No 1341 A -> No 1381 T -> C No 1464 T -> C No 1466 T -> C No 1494 T -> A Yes 1551 A -> G No 1613 T -> C No 1655 G -> A Yes 1668 T -> C No 1682 T -> G Yes 1752 T -> C No 1781 A -> G No 1812 A -> C Yes 1841 T -> C No 1887 A -> No 1977 C -> T No 1994 A -> No 2042 T -> C No 2105 A -> No 2138 A -> No 2138 A -> C No 2227 G -> A No 2641 G -> A Yes 2853 T -> C No 2889 A -> G No 2982 C -> G No 3554 T -> No 3569 A -> C Yes 3829 G -> A No 4027 G -> A Yes

Variant protein HSCP2_PEA_(—)1_P24 (SEQ ID NO: 854) according to the present invention has an amino acid sequence as given at the end of the application; it is encoded by transcript(s) HSCP2_PEA_(—)1_T33 (SEQ ID NO: 791). An alignment is given to the known protein (Ceruloplasmin precursor) at the end of the application. One or more alignments to one or more previously published protein sequences are given at the end of the application. A brief description of the relationship of the variant protein according to the present invention to each such aligned protein is as follows:

Comparison report between HSCP2_PEA_(—)1_P24 (SEQ ID NO: 854) and CERU_HUMAN:

1. An isolated chimeric polypeptide encoding for HSCP2_PEA_(—)1_P24 (SEQ ID NO: 854), comprising a first amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence MPLTMGKRNLFLLTP (SEQ ID NO: 1095) corresponding to amino acids 1-15 of HSCP2_PEA_(—)1_P24 (SEQ ID NO: 854), and a second amino acid sequence being at least 90% homologous to VNGYTFGSLPGLSMCAEDRVKWYLFGMGNEVDVHAAFFHGQALTNKNYRIDTINLFP ATLFDAYMVAQNPGEWMLSCQNLNHLKAGLQAFFQVQECNKSSSKDNIRGKHVRHY YIAAEEIIWNYAPSGIDIFTKENLTAPGSDSAVFFEQGTTRIGGSYKKLVYREYTDASFTN RKERGPEEEHLGILGPVIWAEVGDTIRVTFHNKGAYPLSIEPIGVRFNKNNEGTYYSPNY NPQSRSVPPSASHVAPTETFTYEWTVPKEVGPTNADPVCLAKMYYSAVDPTKDIFTGLI GPMKICKKGSLHANGRQKDVDKEFYLFPTVFDENESLLLEDNIRMFTTAPDQVDKEDE DFQESNKMHSMNGFMYGNQPGLTMCKGDSVVWYLFSAGNEADVHGIYFSGNTYLWR GERRDTANLFPQTSLTLHMWPDTEGTFNVECLTTDHYTGGMKQKYTVNQCRRQSEDS TFYLGERTYYIAAVEVEWDYSPQREWEKELHHLQEQNVSNAFLDKGEFYIGSKYKKVV YRQYTDSTFRVPVERKAEEEHLGILGPQLHADVGDKVKIIFKNMATRPYSIHAHGVQTE SSTVTPTLPGETLTYVWKIPERSGAGTEDSACIPWAYYSTVDQVKDLYSGLIGPLIVCRR PYLKVFNPRRKLEFALLFLVFDENESWYLDDNIKTYSDHPEKVNKDDEEFIESNKMHAI NGRMFGNLQGLTMHVGDEVNWYLMGMGNEIDLHTVHFHGHSFQYKHRGVYSSDVF DIFPGTYQTLEMFPRTPGIWLLHCHVTDHIHAGMETTYTVLQNEDTKSG corresponding to amino acids 262-1065 of CERU_HUMAN, which also corresponds to amino acids 16-819 of HSCP2_PEA_(—)1_P24 (SEQ ID NO: 854), wherein said first amino acid sequence and second amino acid sequence are contiguous and in a sequential order.

2. An isolated polypeptide encoding for a head of HSCP2_PEA_(—)1_P24 (SEQ ID NO: 854), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence MPLTMGKRNLFLLTP (SEQ ID NO: 1095) of HSCP2_PEA_(—)1_P24 (SEQ ID NO: 854).

The location of the variant protein was determined according to results from a number of different software programs and analyses, including analyses from SignalP and other specialized programs. The variant protein is believed to be located as follows with regard to the cell: secreted. The protein localization is believed to be secreted because one of the two signal-peptide prediction programs (HMM:Non-secretory protein,NN:YES) predicts that this protein has a signal peptide.

Variant protein HSCP2_PEA_(—)1_P24 (SEQ ID NO: 854) also has the following non-silent SNPs (Single Nucleotide Polymorphisms) as listed in Table 32, (given according to their position(s) on the amino acid sequence, with the alternative amino acid(s) listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein HSCP2_PEA_(—)1_P24 (SEQ ID NO: 854) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 32 Amino acid mutations SNP position(s) on Alternative amino Previously amino acid sequence acid(s) known SNP? 3 L -> P No 13 L -> No 29 M -> T No 40 F -> L No 52 F -> S No 59 T -> A No 199 H -> Y No 205 P -> A No 231 P -> L No 247 P -> No 261 S -> P No 289 L -> P No 298 D -> E Yes 338 V -> A No 352 R -> K Yes 361 V -> G Yes 394 D -> G No 414 F -> S No 429 A -> No 465 Q -> No 481 F -> S No 502 Q -> No 513 Q -> P No 513 Q -> No 543 D -> N No 681 E -> K Yes 794 C -> W No

The glycosylation sites of variant protein HSCP2_PEA_(—)1_P24 (SEQ ID NO: 854), as compared to the known protein Ceruloplasmin precursor, are described in Table 33 (given according to their position(s) on the amino acid sequence in the first column; the second column indicates whether the glycosylation site is present in the variant protein; and the last column indicates whether the position is different on the variant protein).

TABLE 33 Glycosylation site(s) Position(s) on known amino Present in Position in acid sequence variant protein? variant protein? 138 no 762 yes 516 397 yes 151 358 yes 112

Variant protein HSCP2_PEA_(—)1_P24 (SEQ ID NO: 854) is encoded by the following transcript(s): HSCP2_PEA_(—)1_T33 (SEQ ID NO: 791), for which the sequence(s) is/are given at the end of the application. The coding portion of transcript HSCP2_PEA_(—)1_T33 (SEQ ID NO: 791) is shown in bold; this coding portion starts at position 353 and ends at position 2809. The transcript also has the following SNPs as listed in Table 34 (given according to their position on the nucleotide sequence, with the alternative nucleic acid listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein HSCP2_PEA_(—)1_P24 (SEQ ID NO: 854) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 34 Nucleic acid SNPs SNP position on nucleotide sequence Alternative nucleic acid Previously known SNP? 63 A -> No 201 G -> T No 326 T -> No 335 T -> No 358 T -> C No 360 T -> C No 389 T -> No 418 A -> G Yes 438 T -> C No 472 T -> G No 507 T -> C No 527 A -> G No 649 A -> G No 652 C -> T No 718 G -> A No 947 C -> T No 965 C -> G No 982 G -> A No 1044 C -> T No 1093 A -> No 1133 T -> C No 1216 T -> C No 1218 T -> C No 1246 T -> A Yes 1303 A -> G No 1365 T -> C No 1407 G -> A Yes 1420 T -> C No 1434 T -> G Yes 1504 T -> C No 1533 A -> G No 1564 A -> C Yes 1593 T -> C No 1639 A -> No 1729 C -> T No 1746 A -> No 1794 T -> C No 1857 A -> No 1890 A -> No 1890 A -> C No 1979 G -> A No 2393 G -> A Yes 2605 T -> C No 2641 A -> G No 2734 C -> G No 3306 T -> No 3321 A -> C Yes 3581 G -> A No 3779 G -> A Yes

Variant protein HSCP2_PEA_(—)1_P25 (SEQ ID NO: 855) according to the present invention has an amino acid sequence as given at the end of the application; it is encoded by transcript(s) HSCP2_PEA_(—)1_T34 (SEQ ID NO: 792). An alignment is given to the known protein (Ceruloplasmin precursor) at the end of the application. One or more alignments to one or more previously published protein sequences are given at the end of the application. A brief description of the relationship of the variant protein according to the present invention to each such aligned protein is as follows:

Comparison report between HSCP2_PEA_(—)1_P25 (SEQ ID NO: 855) and CERU_HUMAN:

1. An isolated chimeric polypeptide encoding for HSCP2_PEA_(—)1_P25 (SEQ ID NO: 855), comprising a first amino acid sequence being at least 90% homologous to MKILILGIFLFLCSTPAWAKEKHYYIGIIETTWDYASDHGEKKLISVDTEHSNIYLQNGPD RIGRLYKKALYLQYTDETFRTTIEKPVWLGFLGPIIKAETGDKVYVHLKNLASRPYTFHS HGITYYKEHEGAIYPDNTTDFQRADDKVYPGEQYTYMLLATEEQSPGEGDGNCVTRIY HSHIDAPKDIASGLIGPLIICKKDSLDKEKEKHIDREFVVMFSVVDENFSWYLEDNIKTY CSEPEKVDKDNEDFQESNRMYSVNGYTFGSLPGLSMCAEDRVKWYLFGMGNEVDVH AAFFHGQALTNKNYRIDTINLFPATLFDAYMVAQNPGEWMLSCQNLNHLKAGLQAFF QVQECNKSSSKDNIRGKHVRHYYIAAEEIIWNYAPSGIDIFTKENLTAPGSDSAVFFEQG TTRIGGSYKKLVYREYTDASFTNRKERGPEEEHLGILGPVIWAEVGDTIRVTFHNKGAY PLSIEPIGVRFNKNNEGTYYSPNYNPQSRSVPPSASHVAPTETFTYEWTVPKEVGPTNAD PVCLAKMYYSAVDPTKDIFTGLIGPMKICKKGSLHANGRQKDVDKEFYLFPTVFDENES LLLEDNIRMFTTAPDQVDKEDEDFQESNKMH corresponding to amino acids 1-621 of CERU_HUMAN, which also corresponds to amino acids 1-621 of HSCP2_PEA_(—)1_P25 (SEQ ID NO: 855), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence CKYCIIHQSTKLF (SEQ ID NO: 1096) corresponding to amino acids 622-634 of HSCP2_PEA_(—)1_P25 (SEQ ID NO: 855), wherein said first amino acid sequence and second amino acid sequence are contiguous and in a sequential order.

2. An isolated polypeptide encoding for a tail of HSCP2_PEA_(—)1_P25 (SEQ ID NO: 855), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence CKYCIIHQSTKLF (SEQ ID NO: 1096) in HSCP2_PEA_(—)1_P25 (SEQ ID NO: 855).

The location of the variant protein was determined according to results from a number of different software programs and analyses, including analyses from SignalP and other specialized programs. The variant protein is believed to be located as follows with regard to the cell: secreted. The protein localization is believed to be secreted because both signal-peptide prediction programs predict that this protein has a signal peptide, and neither trans-membrane region prediction program predicts that this protein has a trans-membrane region.

Variant protein HSCP2_PEA_(—)1_P25 (SEQ ID NO: 855) also has the following non-silent SNPs (Single Nucleotide Polymorphisms) as listed in Table 35, (given according to their position(s) on the amino acid sequence, with the alternative amino acid(s) listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein HSCP2_PEA_(—)1_P25 (SEQ ID NO: 855) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 35 Amino acid mutations SNP position(s) on Alternative amino Previously amino acid sequence acid(s) known SNP? 26 I -> No 29 I -> No 37 S -> P No 47 V -> No 54 I -> V No 63 I -> No 92 F -> S No 117 Y -> N No 148 K -> R No 173 N -> No 186 P -> No 190 A -> G No 190 A -> No 213 I -> No 218 V -> M No 221 F -> No 235 N -> D No 253 F -> L No 275 M -> T No 286 F -> L No 298 F -> S No 305 T -> A No 445 H -> Y No 451 P -> A No 477 P -> L No 493 P -> No 507 S -> P No 535 L -> P No 544 D -> E Yes 584 V -> A No 598 R -> K Yes 607 V -> G Yes

The glycosylation sites of variant protein HSCP2_PEA_(—)1_P25 (SEQ ID NO: 855), as compared to the known protein Ceruloplasmin precursor, are described in Table 36 (given according to their position(s) on the amino acid sequence in the first column; the second column indicates whether the glycosylation site is present in the variant protein; and the last column indicates whether the position is different on the variant protein).

TABLE 36 Glycosylation site(s) Position(s) on known amino Present in Position in acid sequence variant protein? variant protein? 138 yes 138 762 no 397 yes 397 358 yes 358

Variant protein HSCP2_PEA_(—)1_P25 (SEQ ID NO: 855) is encoded by the following transcript(s): HSCP2_PEA_(—)1_T34 (SEQ ID NO: 792), for which the sequence(s) is/are given at the end of the application. The coding portion of transcript HSCP2_PEA_(—)1_T34 (SEQ ID NO: 792) is shown in bold; this coding portion starts at position 250 and ends at position 2151. The transcript also has the following SNPs as listed in Table 37 (given according to their position on the nucleotide sequence, with the alternative nucleic acid listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein HSCP2_PEA_(—)1_P25 (SEQ ID NO: 855) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 37 Nucleic acid SNPs SNP position on nucleotide sequence Alternative nucleic acid Previously known SNP? 63 A -> No 201 G -> T No 326 T -> No 335 T -> No 358 T -> C No 360 T -> C No 389 T -> No 409 A -> G No 437 T -> No 524 T -> C No 591 T -> C No 598 T -> A No 692 A -> G No 768 T -> No 807 A -> No 807 A -> G No 818 C -> No 818 C -> G No 837 T -> C No 887 T -> No 901 G -> A No 910 T -> No 952 A -> G No 1006 T -> C No 1053 A -> G Yes 1073 T -> C No 1107 T -> G No 1142 T -> C No 1162 A -> G No 1284 A -> G No 1287 C -> T No 1353 G -> A No 1582 C -> T No 1600 C -> G No 1617 G -> A No 1679 C -> T No 1728 A -> No 1768 T -> C No 1851 T -> C No 1853 T -> C No 1881 T -> A Yes 1938 A -> G No 2000 T -> C No 2042 G -> A Yes 2055 T -> C No 2069 T -> G Yes

Variant protein HSCP2_PEA_(—)1_P33 (SEQ ID NO: 856) according to the present invention has an amino acid sequence as given at the end of the application; it is encoded by transcript(s) HSCP2_PEA_(—)1_T45 (SEQ ID NO: 793). An alignment is given to the known protein (Ceruloplasmin precursor) at the end of the application. One or more alignments to one or more previously published protein sequences are given at the end of the application. A brief description of the relationship of the variant protein according to the present invention to each such aligned protein is as follows:

Comparison report between HSCP2_PEA_(—)1_P33 (SEQ ID NO: 856) and CERU_HUMAN:

1. An isolated chimeric polypeptide encoding for HSCP2_PEA_(—)1_P33 (SEQ ID NO: 856), comprising a first amino acid sequence being at least 90% homologous to MKILILGIFLFLCSTPAWAKEKHYYIGIIETTWDYASDHGEKKLISVDTEHSNIYLQNGPD RIGRLYKKALYLQYTDETFRTTIEKPVWLGFLGPIIKAETGDKVYVHLKNLASRPYTFHS HGITYYKEHEGAIYPDNTTDFQRADDKVYPGEQYTYMLLATEEQSPGEGDGNCVTRIY HSHIDAPKDIASGLIGPLIICKK corresponding to amino acids 1-202 of CERU_HUMAN, which also corresponds to amino acids 1-202 of HSCP2_PEA_(—)1_P33 (SEQ ID NO: 856), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence GTSSPYCTCYMTKRQGQGSLSFKKKSSLLC (SEQ ID NO: 1097) corresponding to amino acids 203-232 of HSCP2_PEA_(—)1_P33 (SEQ ID NO: 856), wherein said first amino acid sequence and second amino acid sequence are contiguous and in a sequential order.

2. An isolated polypeptide encoding for a tail of HSCP2_PEA_(—)1_P33 (SEQ ID NO: 856), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence GTSSPYCTCYMTKRQGQGSLSFKKKSSLLC (SEQ ID NO: 1097) in HSCP2_PEA_(—)1_P33 (SEQ ID NO: 856).

The location of the variant protein was determined according to results from a number of different software programs and analyses, including analyses from SignalP and other specialized programs. The variant protein is believed to be located as follows with regard to the cell: secreted. The protein localization is believed to be secreted because both signal-peptide prediction programs predict that this protein has a signal peptide, and neither trans-membrane region prediction program predicts that this protein has a trans-membrane region.

Variant protein HSCP2_PEA_(—)1_P33 (SEQ ID NO: 856) also has the following non-silent SNPs (Single Nucleotide Polymorphisms) as listed in Table 38, (given according to their position(s) on the amino acid sequence, with the alternative amino acid(s) listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein HSCP2_PEA_(—)1_P33 (SEQ ID NO: 856) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 38 Amino acid mutations SNP position(s) on amino acid sequence Alternative amino acid(s) Previously known SNP? 26 I -> No 29 I -> No 37 S -> P No 47 V -> No 54 I -> V No 63 I -> No 92 F -> S No 117 Y -> N No 148 K -> R No 173 N -> No 186 P -> No 190 A -> G No 190 A -> No

The glycosylation sites of variant protein HSCP2_PEA_(—)1_P33 (SEQ ID NO: 856), as compared to the known protein Ceruloplasmin precursor, are described in Table 39 (given according to their position(s) on the amino acid sequence in the first column; the second column indicates whether the glycosylation site is present in the variant protein; and the last column indicates whether the position is different on the variant protein).

TABLE 39 Glycosylation site(s) Position(s) on known amino Present in Position in acid sequence variant protein? variant protein? 138 yes 138 762 no 397 no 358 no

Variant protein HSCP2_PEA_(—)1_P33 (SEQ ID NO: 856) is encoded by the following transcript(s): HSCP2_PEA_(—)1_T45 (SEQ ID NO: 793), for which the sequence(s) is/are given at the end of the application. The coding portion of transcript HSCP2_PEA_(—)1_T45 (SEQ ID NO: 793) is shown in bold; this coding portion starts at position 250 and ends at position 945. The transcript also has the following SNPs as listed in Table 40 (given according to their position on the nucleotide sequence, with the alternative nucleic acid listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein HSCP2_PEA_(—)1_P33 (SEQ ID NO: 856) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 40 Nucleic acid SNPs SNP position on nucleotide sequence Alternative nucleic acid Previously known SNP? 63 A -> No 201 G -> T No 326 T -> No 335 T -> No 358 T -> C No 360 T -> C No 389 T -> No 409 A -> G No 437 T -> No 524 T -> C No 591 T -> C No 598 T -> A No 692 A -> G No 768 T -> No 807 A -> No 807 A -> G No 818 C -> No 818 C -> G No 837 T -> C No 1099 T -> A Yes

As noted above, cluster HSCP2 features 50 segment(s), which were listed in Table 2 above and for which the sequence(s) are given at the end of the application. These segment(s) are portions of nucleic acid sequence(s) which are described herein separately because they are of particular interest. A description of each segment according to the present invention is now provided.

Segment cluster HSCP2_PEA_(—)1_node_(—)0 (SEQ ID NO: 795) according to the present invention is supported by 53 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HSCP2_PEA_(—)1_T4 (SEQ ID NO: 783), HSCP2_PEA_(—)1_T13 (SEQ ID NO: 784), HSCP2_PEA_(—)1_T19 (SEQ ID NO: 785), HSCP2_PEA_(—)1_T20 (SEQ ID NO: 786), HSCP2_PEA_(—)1_T22 (SEQ ID NO: 787), HSCP2_PEA_(—)1_T23 (SEQ ID NO: 788), HSCP2_PEA_(—)1_T25 (SEQ ID NO: 789), HSCP2_PEA_(—)1_T31 (SEQ ID NO: 790), HSCP2_PEA_(—)1_T33 (SEQ ID NO: 791), HSCP2_PEA_(—)1_T34 (SEQ ID NO: 792), HSCP2_PEA_(—)1_T45 (SEQ ID NO: 793) and HSCP2_PEA_(—)1_T50 (SEQ ID NO: 794). Table 41 below describes the starting and ending position of this segment on each transcript.

TABLE 41 Segment location on transcripts Segment Segment Transcript starting ending name position position HSCP2_PEA_1_T4 1 395 (SEQ ID NO:783) HSCP2_PEA_1_T13 1 395 (SEQ ID NO:784) HSCP2_PEA_1_T19 1 395 (SEQ ID NO:785) HSCP2_PEA_1_T20 1 395 (SEQ ID NO:786) HSCP2_PEA_1_T22 1 395 (SEQ ID NO:787) HSCP2_PEA_1_T23 1 395 (SEQ ID NO:788) HSCP2_PEA_1_T25 1 395 (SEQ ID NO:789) HSCP2_PEA_1_T31 1 395 (SEQ ID NO:790) HSCP2_PEA_1_T33 1 395 (SEQ ID NO:791) HSCP2_PEA_1_T34 1 395 (SEQ ID NO:792) HSCP2_PEA_1_T45 1 395 (SEQ ID NO:793) HSCP2_PEA_1_T50 1 395 (SEQ ID NO:794)

Segment cluster HSCP2_PEA_(—)1_node_(—)3 (SEQ ID NO: 796) according to the present invention is supported by 53 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HSCP2_PEA_(—)1_T4 (SEQ ID NO: 783), HSCP2_PEA_(—)1_T13 (SEQ ID NO: 784), HSCP2_PEA_(—)1_T19 (SEQ ID NO: 785), HSCP2_PEA_(—)1_T20 (SEQ ID NO: 786), HSCP2_PEA_(—)1_T22 (SEQ ID NO: 787), HSCP2_PEA_(—)1_T23 (SEQ ID NO: 788), HSCP2_PEA_(—)1_T25 (SEQ ID NO: 789), HSCP2_PEA_(—)1_T31 (SEQ ID NO: 790), HSCP2_PEA_(—)1_T34 (SEQ ID NO: 792), HSCP2_PEA_(—)1_T45 (SEQ ID NO: 793) and HSCP2_PEA_(—)1_T50 (SEQ ID NO: 794). Table 42 below describes the starting and ending position of this segment on each transcript.

TABLE 42 Segment location on transcripts Segment Segment Transcript starting ending name position position HSCP2_PEA_1_T4 396 587 (SEQ ID NO:783) HSCP2_PEA_1_T13 396 587 (SEQ ID NO:784) HSCP2_PEA_1_T19 396 587 (SEQ ID NO:785) HSCP2_PEA_1_T20 396 587 (SEQ ID NO:786) HSCP2_PEA_1_T22 396 587 (SEQ ID NO:787) HSCP2_PEA_1_T23 396 587 (SEQ ID NO:788) HSCP2_PEA_1_T25 396 587 (SEQ ID NO:789) HSCP2_PEA_1_T31 396 587 (SEQ ID NO:790) HSCP2_PEA_1_T33 396 587 (SEQ ID NO:791) HSCP2_PEA_1_T34 396 587 (SEQ ID NO:792) HSCP2_PEA_1_T45 396 587 (SEQ ID NO:793) HSCP2_PEA_1_T50 396 587 (SEQ ID NO:794)

Segment cluster HSCP2_PEA_(—)1_node_(—)6 (SEQ ID NO: 797) according to the present invention is supported by 63 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HSCP2_PEA_(—)1_T4 (SEQ ID NO: 783), HSCP2_PEA_(—)1_T13 (SEQ ID NO: 784), HSCP2_PEA_(—)1_T19 (SEQ ID NO: 785), HSCP2_PEA_(—)1_T20 (SEQ ID NO: 786), HSCP2_PEA_(—)1_T22 (SEQ ID NO: 787), HSCP2_PEA_(—)1_T23 (SEQ ID NO: 788), HSCP2_PEA_(—)1_T25 (SEQ ID NO: 789), HSCP2_PEA_(—)1_T34 (SEQ ID NO: 792), HSCP2_PEA_(—)1_T45 (SEQ ID NO: 793) and HSCP2_PEA_(—)1_T50 (SEQ ID NO: 794). Table 43 below describes the starting and ending position of this segment on each transcript.

TABLE 43 Segment location on transcripts Segment Segment Transcript starting ending name position position HSCP2_PEA_1_T4 644 830 (SEQ ID NO:783) HSCP2_PEA_1_T13 644 830 (SEQ ID NO:784) HSCP2_PEA_1_T19 644 830 (SEQ ID NO:785) HSCP2_PEA_1_T20 644 830 (SEQ ID NO:786) HSCP2_PEA_1_T22 644 830 (SEQ ID NO:787) HSCP2_PEA_1_T23 644 830 (SEQ ID NO:788) HSCP2_PEA_1_T25 644 830 (SEQ ID NO:789) HSCP2_PEA_1_T34 644 830 (SEQ ID NO:792) HSCP2_PEA_1_T45 644 830 (SEQ ID NO:793) HSCP2_PEA_1_T50 644 830 (SEQ ID NO:794)

Segment cluster HSCP2_PEA_(—)1_node_(—)8 (SEQ ID NO: 798) according to the present invention is supported by 3 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HSCP2_PEA_(—)1_T45 (SEQ ID NO: 793). Table 44 below describes the starting and ending position of this segment on each transcript.

TABLE 44 Segment location on transcripts Segment Segment Transcript starting ending name position position HSCP2_PEA_1_T45 857 1634 (SEQ ID NO:793)

Segment cluster HSCP2_PEA_(—)1_node_(—)10 (SEQ ID NO: 799) according to the present invention is supported by 57 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HSCP2_PEA_(—)1_T4 (SEQ ID NO: 783), HSCP2_PEA_(—)1_T13 (SEQ ID NO: 784), HSCP2_PEA_(—)1_T19 (SEQ ID NO: 785), HSCP2_PEA_(—)1_T20 (SEQ ID NO: 786), HSCP2_PEA_(—)1_T22 (SEQ ID NO: 787), HSCP2_PEA_(—)1_T23 (SEQ ID NO: 788), HSCP2_PEA_(—)1_T25 (SEQ ID NO: 789), HSCP2_PEA_(—)1_T34 (SEQ ID NO: 792) and HSCP2_PEA_(—)1_T50 (SEQ ID NO: 794). Table 45 below describes the starting and ending position of this segment on each transcript.

TABLE 45 Segment location on transcripts Segment Segment Transcript name starting position ending position HSCP2_PEA_1_T4 857 1030 (SEQ ID NO: 783) HSCP2_PEA_1_T13 857 1030 (SEQ ID NO: 784) HSCP2_PEA_1_T19 857 1030 (SEQ ID NO: 785) HSCP2_PEA_1_T20 857 1030 (SEQ ID NO: 786) HSCP2_PEA_1_T22 857 1030 (SEQ ID NO: 787) HSCP2_PEA_1_T23 857 1030 (SEQ ID NO: 788) HSCP2_PEA_1_T25 857 1030 (SEQ ID NO: 789) HSCP2_PEA_1_T34 857 1030 (SEQ ID NO: 792) HSCP2_PEA_1_T50 857 1030 (SEQ ID NO: 794)

Segment cluster HSCP2_PEA_(—)1_node_(—)14 (SEQ ID NO: 800) according to the present invention is supported by 49 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HSCP2_PEA_(—)1_T4 (SEQ ID NO: 783), HSCP2_PEA_(—)1_T13 (SEQ ID NO: 784), HSCP2_PEA_(—)1_T19 (SEQ ID NO: 785), HSCP2_PEA_(—)1_T20 (SEQ ID NO: 786), HSCP2_PEA_(—)1_T22 (SEQ ID NO: 787), HSCP2_PEA_(—)1_T23 (SEQ ID NO: 788), HSCP2_PEA_(—)1_T25 (SEQ ID NO: 789), HSCP2_PEA_(—)1_T31 (SEQ ID NO: 790), HSCP2_PEA_(—)1_T33 (SEQ ID NO: 791), HSCP2_PEA_(—)1_T34 (SEQ ID NO: 792) and HSCP2_PEA_(—)1_T50 (SEQ ID NO: 794). Table 46 below describes the starting and ending position of this segment on each transcript.

TABLE 46 Segment location on transcripts Segment Segment Transcript starting ending name position position HSCP2_PEA_1_T4 1089 1236 (SEQ ID NO:783) HSCP2_PEA_1_T13 1089 1236 (SEQ ID NO:784) HSCP2_PEA_1_T19 1089 1236 (SEQ ID NO:785) HSCP2_PEA_1_T20 1089 1236 (SEQ ID NO:786) HSCP2_PEA_1_T22 1089 1236 (SEQ ID NO:787) HSCP2_PEA_1_T23 1089 1236 (SEQ ID NO:788) HSCP2_PEA_1_T25 1089 1236 (SEQ ID NO:789) HSCP2_PEA_1_T31 702 849 (SEQ ID NO:790) HSCP2_PEA_1_T33 454 601 (SEQ ID NO:791) HSCP2_PEA_1_T34 1089 1236 (SEQ ID NO:792) HSCP2_PEA_1_T50 1089 1236 (SEQ ID NO:794)

Segment cluster HSCP2_PEA_(—)1_node_(—)23 (SEQ ID NO: 801) according to the present invention is supported by 58 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HSCP2_PEA_(—)1_T4 (SEQ ID NO: 783), HSCP2_PEA_(—)1_T13 (SEQ ID NO: 784), HSCP2_PEA_(—)1_T19 (SEQ ID NO: 785), HSCP2_PEA_(—)1_T20 (SEQ ID NO: 786), HSCP2_PEA_(—)1_T22 (SEQ ID NO: 787), HSCP2_PEA_(—)1_T23 (SEQ ID NO: 788), HSCP2_PEA_(—)1_T25 (SEQ ID NO: 789), HSCP2_PEA_(—)1_T31 (SEQ ID NO: 790), HSCP2_PEA_(—)1_T33 (SEQ ID NO: 791), HSCP2_PEA_(—)1_T34 (SEQ ID NO: 792) and HSCP2_PEA_(—)1_T50 (SEQ ID NO: 794). Table 47 below describes the starting and ending position of this segment on each transcript.

TABLE 47 Segment location on transcripts Segment Segment Transcript starting ending name position position HSCP2_PEA_1_T4 1458 1597 (SEQ ID NO:783) HSCP2_PEA_1_T13 1458 1597 (SEQ ID NO:784) HSCP2_PEA_1_T19 1458 1597 (SEQ ID NO:785) HSCP2_PEA_1_T20 1458 1597 (SEQ ID NO:786) HSCP2_PEA_1_T22 1458 1597 (SEQ ID NO:787) HSCP2_PEA_1_T23 1458 1597 (SEQ ID NO:788) HSCP2_PEA_1_T25 1458 1597 (SEQ ID NO:789) HSCP2_PEA_1_T31 1071 1210 (SEQ ID NO:790) HSCP2_PEA_1_T33 823 962 (SEQ ID NO:791) HSCP2_PEA_1_T34 1458 1597 (SEQ ID NO:792) HSCP2_PEA_1_T50 1458 1597 (SEQ ID NO:794)

Segment cluster HSCP2_PEA_(—)1_node_(—)26 (SEQ ID NO: 802) according to the present invention is supported by 67 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HSCP2_PEA_(—)1_T4 (SEQ ID NO: 783), HSCP2_PEA_(—)1_T13 (SEQ ID NO: 784), HSCP2_PEA_(—)1_T19 (SEQ ID NO: 785), HSCP2_PEA_(—)1_T20 (SEQ ID NO: 786), HSCP2_PEA_(—)1_T22 (SEQ ID NO: 787), HSCP2_PEA_(—)1_T23 (SEQ ID NO: 788), HSCP2_PEA_(—)1_T25 (SEQ ID NO: 789), HSCP2_PEA_(—)1_T31 (SEQ ID NO: 790), HSCP2_PEA_(—)1_T33 (SEQ ID NO: 791), HSCP2_PEA_(—)1_T34 (SEQ ID NO: 792) and HSCP2_PEA_(—)1_T50 (SEQ ID NO: 794). Table 48 below describes the starting and ending position of this segment on each transcript.

TABLE 48 Segment location on transcripts Segment Segment Transcript starting ending name position position HSCP2_PEA_1_T4 1598 1750 (SEQ ID NO:783) HSCP2_PEA_1_T13 1598 1750 (SEQ ID NO:784) HSCP2_PEA_1_T19 1598 1750 (SEQ ID NO:785) HSCP2_PEA_1_T20 1598 1750 (SEQ ID NO:786) HSCP2_PEA_1_T22 1598 1750 (SEQ ID NO:787) HSCP2_PEA_1_T23 1598 1750 (SEQ ID NO:788) HSCP2_PEA_1_T25 1598 1750 (SEQ ID NO:789) HSCP2_PEA_1_T31 1211 1363 (SEQ ID NO:790) HSCP2_PEA_1_T33 963 1115 (SEQ ID NO:791) HSCP2_PEA_1_T34 1598 1750 (SEQ ID NO:792) HSCP2_PEA_1_T50 1598 1750 (SEQ ID NO:794)

Segment cluster HSCP2_PEA_(—)1_node_(—)29 (SEQ ID NO: 803) according to the present invention is supported by 64 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HSCP2_PEA_(—)1_T4 (SEQ ID NO: 783), HSCP2_PEA_(—)1_T13 (SEQ ID NO: 784), HSCP2_PEA_(—)1_T19 (SEQ ID NO: 785), HSCP2_PEA_(—)1_T20 (SEQ ID NO: 786), HSCP2_PEA_(—)1_T22 (SEQ ID NO: 787), HSCP2_PEA_(—)1_T23 (SEQ ID NO: 788), HSCP2_PEA_(—)1_T25 (SEQ ID NO: 789), HSCP2_PEA_(—)1_T31 (SEQ ID NO: 790), HSCP2_PEA_(—)1_T33 (SEQ ID NO: 791), HSCP2_PEA_(—)1_T34 (SEQ ID NO: 792) and HSCP2_PEA_(—)1_T50 (SEQ ID NO: 794). Table 49 below describes the starting and ending position of this segment on each transcript.

TABLE 49 Segment location on transcripts Segment Segment Transcript starting ending name position position HSCP2_PEA_1_T4 1751 1962 (SEQ ID NO: 783) HSCP2_PEA_1_T13 1751 1962 (SEQ ID NO: 784) HSCP2_PEA_1_T19 1751 1962 (SEQ ID NO: 785) HSCP2_PEA_1_T20 1751 1962 (SEQ ID NO: 786) HSCP2_PEA_1_T22 1751 1962 (SEQ ID NO: 787) HSCP2_PEA_1_T23 1751 1962 (SEQ ID NO: 788) HSCP2_PEA_1_T25 1751 1962 (SEQ ID NO: 789) HSCP2_PEA_1_T31 1364 1575 (SEQ ID NO: 790) HSCP2_PEA_1_T33 1116 1327 (SEQ ID NO: 791) HSCP2_PEA_1_T34 1751 1962 (SEQ ID NO: 792) HSCP2_PEA_1_T50 1751 1962 (SEQ ID NO: 794)

Segment cluster HSCP2_PEA_(—)1_node_(—)31 (SEQ ID NO: 804) according to the present invention is supported by 72 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HSCP2_PEA_(—)1_T4 (SEQ ID NO: 783), HSCP2_PEA_(—)1_T13 (SEQ ID NO: 784), HSCP2_PEA_(—)1_T19 (SEQ ID NO: 785), HSCP2_PEA_(—)1_T20 (SEQ ID NO: 786), HSCP2_PEA_(—)1_T22 (SEQ ID NO: 787), HSCP2_PEA_(—)1_T23 (SEQ ID NO: 788), HSCP2_PEA_(—)1_T25 (SEQ ID NO: 789), HSCP2_PEA_(—)1_T31 (SEQ ID NO: 790), HSCP2_PEA_(—)1_T33 (SEQ ID NO: 791), HSCP2_PEA_(—)1_T34 (SEQ ID NO: 792) and HSCP2_PEA_(—)1_T50 (SEQ ID NO: 794). Table 50 below describes the starting and ending position of this segment on each transcript.

TABLE 50 Segment location on transcripts Segment Segment Transcript name starting position ending position HSCP2_PEA_1_T4 1963 2113 (SEQ ID NO: 783) HSCP2_PEA_1_T13 1963 2113 (SEQ ID NO: 784) HSCP2_PEA_1_T19 1963 2113 (SEQ ID NO: 785) HSCP2_PEA_1_T20 1963 2113 (SEQ ID NO: 786) HSCP2_PEA_1_T22 1963 2113 (SEQ ID NO: 787) HSCP2_PEA_1_T23 1963 2113 (SEQ ID NO: 788) HSCP2_PEA_1_T25 1963 2113 (SEQ ID NO: 789) HSCP2_PEA_1_T31 1576 1726 (SEQ ID NO: 790) HSCP2_PEA_1_T33 1328 1478 (SEQ ID NO: 791) HSCP2_PEA_1_T34 1963 2113 (SEQ ID NO: 792) HSCP2_PEA_1_T50 1963 2113 (SEQ ID NO: 794)

Segment cluster HSCP2_PEA_(—)1_node_(—)32 (SEQ ID NO: 805) according to the present invention is supported by 5 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HSCP2_PEA_(—)1_T34 (SEQ ID NO: 792). Table 51 below describes the starting and ending position of this segment on each transcript.

TABLE 51 Segment location on transcripts Segment Segment Transcript starting ending name position position HSCP2_PEA_1_T34 2114 2246 (SEQ ID NO: 792)

Segment cluster HSCP2_PEA_(—)1_node_(—)34 (SEQ ID NO: 806) according to the present invention is supported by 65 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HSCP2_PEA_(—)1_T4 (SEQ ID NO: 783), HSCP2_PEA_(—)1_T13 (SEQ ID NO: 784), HSCP2_PEA_(—)1_T20 (SEQ ID NO: 786), HSCP2_PEA_(—)1_T22 (SEQ ID NO: 787), HSCP2_PEA_(—)1_T23 (SEQ ID NO: 788), HSCP2_PEA_(—)1_T25 (SEQ ID NO: 789), HSCP2_PEA_(—)1_T31 (SEQ ID NO: 790), HSCP2_PEA_(—)1_T33 (SEQ ID NO: 791) and HSCP2_PEA_(—)1_T50 (SEQ ID NO: 794). Table 52 below describes the starting and ending position of this segment on each transcript.

TABLE 52 Segment location on transcripts Segment Segment Transcript starting ending name position position HSCP2_PEA_1_T4 2114 2326 (SEQ ID NO: 783) HSCP2_PEA_1_T13 2114 2326 (SEQ ID NO: 784) HSCP2_PEA_1_T20 2114 2326 (SEQ ID NO: 786) HSCP2_PEA_1_T22 2114 2326 (SEQ ID NO: 787) HSCP2_PEA_1_T23 2114 2326 (SEQ ID NO: 788) HSCP2_PEA_1_T25 2114 2326 (SEQ ID NO: 789) HSCP2_PEA_1_T31 1727 1939 (SEQ ID NO: 790) HSCP2_PEA_1_T33 1479 1691 (SEQ ID NO: 791) HSCP2_PEA_1_T50 2114 2326 (SEQ ID NO: 794)

Segment cluster HSCP2_PEA_(—)1_node_(—)52 (SEQ ID NO: 807) according to the present invention is supported by 8 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HSCP2_PEA_(—)1_T22 (SEQ ID NO: 787). Table 53 below describes the starting and ending position of this segment on each transcript.

TABLE 53 Segment location on transcripts Segment Segment Transcript starting ending name position position HSCP2_PEA_1_T22 2866 4061 (SEQ ID NO: 787)

Segment cluster HSCP2_PEA_(—)1_node_(—)58 (SEQ ID NO: 808) according to the present invention is supported by 89 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HSCP2_PEA_(—)1_T4 (SEQ ID NO: 783), HSCP2_PEA_(—)1_T13 (SEQ ID NO: 784), HSCP2_PEA_(—)1_T19 (SEQ ID NO: 785), HSCP2_PEA_(—)1_T20 (SEQ ID NO: 786), HSCP2_PEA_(—)1_T23 (SEQ ID NO: 788), HSCP2_PEA_(—)1_T25 (SEQ ID NO: 789), HSCP2_PEA_(—)1_T31 (SEQ ID NO: 790), HSCP2_PEA_(—)1_T33 (SEQ ID NO: 791) and HSCP2_PEA_(—)1_T50 (SEQ ID NO: 794). Table 54 below describes the starting and ending position of this segment on each transcript.

TABLE 54 Segment location on transcripts Segment Segment Transcript starting ending name position position HSCP2_PEA_1_T4 2911 3127 (SEQ ID NO: 783) HSCP2_PEA_1_T13 2911 3127 (SEQ ID NO: 784) HSCP2_PEA_1_T19 2698 2914 (SEQ ID NO: 785) HSCP2_PEA_1_T20 2911 3127 (SEQ ID NO: 786) HSCP2_PEA_1_T23 2911 3127 (SEQ ID NO: 788) HSCP2_PEA_1_T25 2911 3127 (SEQ ID NO: 789) HSCP2_PEA_1_T31 2524 2740 (SEQ ID NO: 790) HSCP2_PEA_1_T33 2276 2492 (SEQ ID NO: 791) HSCP2_PEA_1_T50 2911 3127 (SEQ ID NO: 794)

Segment cluster HSCP2_PEA_(—)1_node_(—)72 (SEQ ID NO: 809) according to the present invention is supported by 2 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HSCP2_PEA_(—)1_T4 (SEQ ID NO: 783) and HSCP2_PEA_(—)1_T50 (SEQ ID NO: 794). Table 55 below describes the starting and ending position of this segment on each transcript.

TABLE 55 Segment location on transcripts Segment Segment Transcript starting ending name position position HSCP2_PEA_1_T4 3431 3636 (SEQ ID NO: 783) HSCP2_PEA_1_T50 3431 3636 (SEQ ID NO: 794)

Segment cluster HSCP2_PEA_(—)1_node_(—)73 (SEQ ID NO: 810) according to the present invention is supported by 28 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HSCP2_PEA_(—)1_T4 (SEQ ID NO: 783). Table 56 below describes the starting and ending position of this segment on each transcript.

TABLE 56 Segment location on transcripts Segment Segment Transcript starting ending name position position HSCP2_PEA_1_T4 3637 5580 (SEQ ID NO: 783)

Segment cluster HSCP2_PEA_(—)1_node_(—)74 (SEQ ID NO: 811) according to the present invention is supported by 86 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HSCP2_PEA_(—)1_T4 (SEQ ID NO: 783), HSCP2_PEA_(—)1_T13 (SEQ ID NO: 784), HSCP2_PEA_(—)1_T19 (SEQ ID NO: 785), HSCP2_PEA_(—)1_T25 (SEQ ID NO: 789), HSCP2_PEA_(—)1_T31 (SEQ ID NO: 790) and HSCP2_PEA_(—)1_T33 (SEQ ID NO: 791). Table 57 below describes the starting and ending position of this segment on each transcript.

TABLE 57 Segment location on transcripts Segment Segment Transcript starting ending name position position HSCP2_PEA_1_T4 5581 5882 (SEQ ID NO: 783) HSCP2_PEA_1_T13 3370 3671 (SEQ ID NO: 784) HSCP2_PEA_1_T19 3218 3519 (SEQ ID NO: 785) HSCP2_PEA_1_T25 3426 3568 (SEQ ID NO: 789) HSCP2_PEA_1_T31 3044 3345 (SEQ ID NO: 790) HSCP2_PEA_1_T33 2796 3097 (SEQ ID NO: 791)

Segment cluster HSCP2_PEA_(—)1_node_(—)76 (SEQ ID NO: 812) according to the present invention is supported by 69 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HSCP2_PEA_(—)1_T4 (SEQ ID NO: 783), HSCP2_PEA_(—)1_T13 (SEQ ID NO: 784), HSCP2_PEA_(—)1_T19 (SEQ ID NO: 785), HSCP2_PEA_(—)1_T31 (SEQ ID NO: 790) and HSCP2_PEA_(—)1_T33 (SEQ ID NO: 791). Table 58 below describes the starting and ending position of this segment on each transcript.

TABLE 58 Segment location on transcripts Segment Segment Transcript starting ending name position position HSCP2_PEA_1_T4 5936 6215 (SEQ ID NO: 783) HSCP2_PEA_1_T13 3725 4004 (SEQ ID NO: 784) HSCP2_PEA_1_T19 3573 3852 (SEQ ID NO: 785) HSCP2_PEA_1_T31 3399 3678 (SEQ ID NO: 790) HSCP2_PEA_1_T33 3151 3430 (SEQ ID NO: 791)

Segment cluster HSCP2_PEA_(—)1_node_(—)78 (SEQ ID NO: 813) according to the present invention is supported by 67 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HSCP2_PEA_(—)1_T4 (SEQ ID NO: 783), HSCP2_PEA_(—)1_T13 (SEQ ID NO: 784), HSCP2_PEA_(—)1_T19 (SEQ ID NO: 785), HSCP2_PEA_(—)1_T31 (SEQ ID NO: 790) and HSCP2_PEA_(—)1_T33 (SEQ ID NO: 791). Table 59 below describes the starting and ending position of this segment on each transcript.

TABLE 59 Segment location on transcripts Segment Segment Transcript starting ending name position position HSCP2_PEA_1_T4 6270 6494 (SEQ ID NO: 783) HSCP2_PEA_1_T13 4059 4283 (SEQ ID NO: 784) HSCP2_PEA_1_T19 3907 4131 (SEQ ID NO: 785) HSCP2_PEA_1_T31 3733 3957 (SEQ ID NO: 790) HSCP2_PEA_1_T33 3485 3709 (SEQ ID NO: 791)

Segment cluster HSCP2_PEA_(—)1_node_(—)80 (SEQ ID NO: 814) according to the present invention is supported by 59 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HSCP2_PEA_(—)1_T4 (SEQ ID NO: 783), HSCP2_PEA_(—)1_T13 (SEQ ID NO: 784), HSCP2_PEA_(—)1_T19 (SEQ ID NO: 785), HSCP2_PEA_(—)1_T31 (SEQ ID NO: 790) and HSCP2_PEA_(—)1_T33 (SEQ ID NO: 791). Table 60 below describes the starting and ending position of this segment on each transcript.

TABLE 60 Segment location on transcripts Segment Segment Transcript starting starting name position position HSCP2_PEA_1_T4 6549 6807 (SEQ ID NO: 783) HSCP2_PEA_1_T13 4338 4596 (SEQ ID NO: 784) HSCP2_PEA_1_T19 4186 4444 (SEQ ID NO: 785) HSCP2_PEA_1_T31 4012 4270 (SEQ ID NO: 790) HSCP2_PEA_1_T33 3764 4022 (SEQ ID NO: 791)

Segment cluster HSCP2_PEA_(—)1_node_(—)84 (SEQ ID NO. 815) according to the present invention is supported by 55 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HSCP2_PEA_(—)1_T20 (SEQ ID NO: 786) and HSCP2_PEA_(—)1_T23 (SEQ ID NO: 788). Table 61 below describes the starting and ending position of this segment on each transcript.

TABLE 61 Segment location on transcripts Segment Segment Transcript starting ending name position position HSCP2_PEA_1_T20 3548 4013 (SEQ ID NO: 786) HSCP2_PEA_1_T23 3373 3838 (SEQ ID NO: 788)

According to an optional embodiment of the present invention, short segments related to the above cluster are also provided. These segments are up to about 120 bp in length, and so are included in a separate description.

Segment cluster HSCP2_PEA_(—)1_node_(—)4 (SEQ ID NO: 816) according to the present invention is supported by 51 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HSCP2_PEA_(—)1_T4 (SEQ ID NO: 783), HSCP2_PEA_(—)1_T13 (SEQ ID NO: 784), HSCP2_PEA_(—)1_T19 (SEQ ID NO: 785), HSCP2_PEA_(—)1_T20 (SEQ ID NO: 786), HSCP2_PEA_(—)1_T22 (SEQ ID NO: 787), HSCP2_PEA_(—)1_T23 (SEQ ID NO: 788), HSCP2_PEA_(—)1_T25 (SEQ ID NO: 789), HSCP2_PEA_(—)1_T31 (SEQ ID NO: 790), HSCP2_PEA_(—)1_T34 (SEQ ID NO: 792), HSCP2_PEA_(—)1_T45 (SEQ ID NO: 793) and HSCP2_PEA_(—)1_T50 (SEQ ID NO: 794). Table 62 below describes the starting and ending position of this segment on each transcript.

TABLE 62 Segment location on transcripts Segment Segment Transcript starting ending name position position HSCP2_PEA_1_T4 588 643 (SEQ ID NO: 783) HSCP2_PEA_1_T13 588 643 (SEQ ID NO: 784) HSCP2_PEA_1_T19 588 643 (SEQ ID NO: 785) HSCP2_PEA_1_T20 588 643 (SEQ ID NO: 786) HSCP2_PEA_1_T22 588 643 (SEQ ID NO: 787) HSCP2_PEA_1_T23 588 643 (SEQ ID NO: 788) HSCP2_PEA_1_T25 588 643 (SEQ ID NO: 789) HSCP2_PEA_1_T31 588 643 (SEQ ID NO: 790) HSCP2_PEA_1_T34 588 643 (SEQ ID NO: 792) HSCP2_PEA_1_T45 588 643 (SEQ ID NO: 793) HSCP2_PEA_1_T50 588 643 (SEQ ID NO: 794)

Segment cluster HSCP2_PEA_(—)1_node_(—)7 (SEQ ID NO: 817) according to the present invention is supported by 56 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HSCP2_PEA_(—)1_T4 (SEQ ID NO: 783), HSCP2_PEA_(—)1_T13 (SEQ ID NO: 784), HSCP2_PEA_(—)1_T19 (SEQ ID NO: 785), HSCP2_PEA_(—)1_T20 (SEQ ID NO: 786), HSCP2_PEA_(—)1_T22 (SEQ ID NO: 787), HSCP2_PEA_(—)1_T23 (SEQ ID NO: 788), HSCP2_PEA_(—)1_T25 (SEQ ID NO: 789), HSCP2_PEA_(—)1_T34 (SEQ ID NO: 792), HSCP2_PEA_(—)1_T45 (SEQ ID NO: 793) and HSCP2_PEA_(—)1_T50 (SEQ ID NO: 794). Table 63 below describes the starting and ending position of this segment on each transcript.

TABLE 63 Segment location on transcripts Segment Segment Transcript starting ending name position position HSCP2_PEA_1_T4 831 856 (SEQ ID NO: 783) HSCP2_PEA_1_T13 831 856 (SEQ ID NO: 784) HSCP2_PEA_1_T19 831 856 (SEQ ID NO: 785) HSCP2_PEA_1_T20 831 856 (SEQ ID NO: 786) HSCP2_PEA_1_T22 831 856 (SEQ ID NO: 787) HSCP2_PEA_1_T23 831 856 (SEQ ID NO: 788) HSCP2_PEA_1_T25 831 856 (SEQ ID NO: 789) HSCP2_PEA_1_T34 831 856 (SEQ ID NO: 792) HSCP2_PEA_1_T45 831 856 (SEQ ID NO: 793) HSCP2_PEA_1_T50 831 856 (SEQ ID NO: 794)

Segment cluster HSCP2_PEA_(—)1_node_(—)13 (SEQ ID NO: 818) according to the present invention is supported by 46 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HSCP2_PEA_(—)1_T4 (SEQ ID NO: 783), HSCP2_PEA_(—)1_T13 (SEQ ID NO: 784), HSCP2_PEA_(—)1_T19 (SEQ ID NO: 785), HSCP2_PEA_(—)1_T20 (SEQ ID NO: 786), HSCP2_PEA_(—)1_T22 (SEQ ID NO: 787), HSCP2_PEA_(—)1_T23 (SEQ ID NO: 788), HSCP2_PEA_(—)1_T25 (SEQ ID NO: 789), HSCP2_PEA_(—)1_T31 (SEQ ID NO: 790), HSCP2_PEA_(—)1_T33 (SEQ ID NO: 791), HSCP2_PEA_(—)1_T34 (SEQ ID NO: 792) and HSCP2_PEA_(—)1_T50 (SEQ ID NO: 794). Table 64 below describes the starting and ending position of this segment on each transcript.

TABLE 64 Segment location on transcripts Segment Segment Transcript starting ending name position position HSCP2_PEA_1_T4 1031 1088 (SEQ ID NO: 783) HSCP2_PEA_1_T13 1031 1088 (SEQ ID NO: 784) HSCP2_PEA_1_T19 1031 1088 (SEQ ID NO: 785) HSCP2_PEA_1_T20 1031 1088 (SEQ ID NO: 786) HSCP2_PEA_1_T22 1031 1088 (SEQ ID NO: 787) HSCP2_PEA_1_T23 1031 1088 (SEQ ID NO: 788) HSCP2_PEA_1_T25 1031 1088 (SEQ ID NO: 789) HSCP2_PEA_1_T31 644 701 (SEQ ID NO: 790) HSCP2_PEA_1_T33 396 453 (SEQ ID NO: 791) HSCP2_PEA_1_T34 1031 1088 (SEQ ID NO: 792) HSCP2_PEA_1_T50 1031 1088 (SEQ ID NO: 794)

Segment cluster HSCP2_PEA_(—)1_node_(—)15 (SEQ ID NO: 819) according to the present invention is supported by 46 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HSCP2_PEA_(—)1_T4 (SEQ ID NO: 783), HSCP2_PEA_(—)1_T13 (SEQ ID NO: 784), HSCP2_PEA_(—)1_T19 (SEQ ID NO: 785), HSCP2_PEA_(—)1_T20 (SEQ ID NO: 786), HSCP2_PEA_(—)1_T22 (SEQ ID NO: 787), HSCP2_PEA_(—)1_T23 (SEQ ID NO: 788), HSCP2_PEA_(—)1_T25 (SEQ ID NO: 789), HSCP2_PEA_(—)1_T31 (SEQ ID NO: 790), HSCP2_PEA_(—)1_T33 (SEQ ID NO: 791), HSCP2_PEA_(—)1_T34 (SEQ ID NO: 792) and HSCP2_PEA_(—)1_T50 (SEQ ID NO: 794). Table 65 below describes the starting and ending position of this segment on each transcript.

TABLE 65 Segment location on transcripts Segment Segment Transcript starting ending name position position HSCP2_PEA_1_T4 1237 1272 (SEQ ID NO: 783) HSCP2_PEA_1_T13 1237 1272 (SEQ ID NO: 784) HSCP2_PEA_1_T19 1237 1272 (SEQ ID NO: 785) HSCP2_PEA_1_T20 1237 1272 (SEQ ID NO: 786) HSCP2_PEA_1_T22 1237 1272 (SEQ ID NO: 787) HSCP2_PEA_1_T23 1237 1272 (SEQ ID NO: 788) HSCP2_PEA_1_T25 1237 1272 (SEQ ID NO: 789) HSCP2_PEA_1_T31 850 885 (SEQ ID NO: 790) HSCP2_PEA_1_T33 602 637 (SEQ ID NO: 791) HSCP2_PEA_1_T34 1237 1272 (SEQ ID NO: 792) HSCP2_PEA_1_T50 1237 1272 (SEQ ID NO: 794)

Segment cluster HSCP2_PEA_(—)1_node_(—)16 (SEQ ID NO: 820) according to the present invention can be found in the following transcript(s): HSCP2_PEA_(—)1_T4 (SEQ ID NO: 783), HSCP2_PEA_(—)1_T13 (SEQ ID NO: 784), HSCP2_PEA_(—)1_T19 (SEQ ID NO: 785), HSCP2_PEA_(—)1_T20 (SEQ ID NO: 786), HSCP2_PEA_(—)1_T22 (SEQ ID NO: 787), HSCP2_PEA_(—)1_T23 (SEQ ID NO: 788), HSCP2_PEA_(—)1_T25 (SEQ ID NO: 789), HSCP2_PEA_(—)1_T31 (SEQ ID NO: 790), HSCP2_PEA_(—)1_T33 (SEQ ID NO: 791), HSCP2_PEA_(—)1_T34 (SEQ ID NO: 792) and HSCP2_PEA_(—)1_T50 (SEQ ID NO: 794). Table 66 below describes the starting and ending position of this segment on each transcript.

TABLE 66 Segment location on transcripts Segment Segment Transcript starting ending name position position HSCP2_PEA_1_T4 1273 1285 (SEQ ID NO: 783) HSCP2_PEA_1_T13 1273 1285 (SEQ ID NO: 784) HSCP2_PEA_1_T19 1273 1285 (SEQ ID NO: 785) HSCP2_PEA_1_T20 1273 1285 (SEQ ID NO: 786) HSCP2_PEA_1_T22 1273 1285 (SEQ ID NO: 787) HSCP2_PEA_1_T23 1273 1285 (SEQ ID NO: 788) HSCP2_PEA_1_T25 1273 1285 (SEQ ID NO: 789) HSCP2_PEA_1_T31 886 898 (SEQ ID NO: 790) HSCP2_PEA_1_T33 638 650 (SEQ ID NO: 791) HSCP2_PEA_1_T34 1273 1285 (SEQ ID NO: 792) HSCP2_PEA_1_T50 1273 1285 (SEQ ID NO: 794)

Segment cluster HSCP2_PEA_(—)1_node_(—)18 (SEQ ID NO: 821) according to the present invention can be found in the following transcript(s): HSCP2_PEA_(—)1_T4 (SEQ ID NO: 783), HSCP2_PEA_(—)1_T13 (SEQ ID NO: 784), HSCP2_PEA_(—)1_T19 (SEQ ID NO: 785), HSCP2_PEA_(—)1_T20 (SEQ ID NO: 786), HSCP2_PEA_(—)1_T22 (SEQ ID NO: 787), HSCP2_PEA_(—)1_T23 (SEQ ID NO: 788), HSCP2_PEA_(—)1_T25 (SEQ ID NO: 789), HSCP2_PEA_(—)1_T31 (SEQ ID NO: 790), HSCP2_PEA_(—)1_T33 (SEQ ID NO: 791), HSCP2_PEA_(—)1_T34 (SEQ ID NO: 792) and HSCP2_PEA_(—)1_T50 (SEQ ID NO: 794). Table 67 below describes the starting and ending position of this segment on each transcript.

TABLE 67 Segment location on transcripts Segment Segment Transcript starting ending name position position HSCP2_PEA_1_T4 1286 1308 (SEQ ID NO: 783) HSCP2_PEA_1_T13 1286 1308 (SEQ ID NO: 784) HSCP2_PEA_1_T19 1286 1308 (SEQ ID NO: 785) HSCP2_PEA_1_T20 1286 1308 (SEQ ID NO: 786) HSCP2_PEA_1_T22 1286 1308 (SEQ ID NO: 787) HSCP2_PEA_1_T23 1286 1308 (SEQ ID NO: 788) HSCP2_PEA_1_T25 1286 1308 (SEQ ID NO: 789) HSCP2_PEA_1_T31 899 921 (SEQ ID NO: 790) HSCP2_PEA_1_T33 651 673 (SEQ ID NO: 791) HSCP2_PEA_1_T34 1286 1308 (SEQ ID NO: 792) HSCP2_PEA_1_T50 1286 1308 (SEQ ID NO: 794)

Segment cluster HSCP2_PEA_(—)1_node_(—)20 (SEQ ID NO: 822) according to the present invention is supported by 48 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HSCP2_PEA_(—)1_T4 (SEQ ID NO: 783), HSCP2_PEA_(—)1_T13 (SEQ ID NO: 784), HSCP2_PEA_(—)1_T19 (SEQ ID NO: 785), HSCP2_PEA_(—)1_T20 (SEQ ID NO: 786), HSCP2_PEA_(—)1_T22 (SEQ ID NO: 787), HSCP2_PEA_(—)1_T23 (SEQ ID NO: 788), HSCP2_PEA_(—)1_T25 (SEQ ID NO: 789), HSCP2_PEA_(—)1_T31 (SEQ ID NO: 790), HSCP2_PEA_(—)1_T33 (SEQ ID NO: 791), HSCP2_PEA_(—)1_T34 (SEQ ID NO: 792) and HSCP2_PEA_(—)1_T50 (SEQ ID NO: 794). Table 68 below describes the starting and ending position of this segment on each transcript.

TABLE 68 Segment location on transcripts Segment Segment Transcript starting ending name position position HSCP2_PEA_1_T4 1309 1374 (SEQ ID NO: 783) HSCP2_PEA_1_T13 1309 1374 (SEQ ID NO: 784) HSCP2_PEA_1_T19 1309 1374 (SEQ ID NO: 785) HSCP2_PEA_1_T20 1309 1374 (SEQ ID NO: 786) HSCP2_PEA_1_T22 1309 1374 (SEQ ID NO: 787) HSCP2_PEA_1_T23 1309 1374 (SEQ ID NO: 788) HSCP2_PEA_1_T25 1309 1374 (SEQ ID NO: 789) HSCP2_PEA_1_T31 922 987 (SEQ ID NO: 790) HSCP2_PEA_1_T33 674 739 (SEQ ID NO: 791) HSCP2_PEA_1_T34 1309 1374 (SEQ ID NO: 792) HSCP2_PEA_1_T50 1309 1374 (SEQ ID NO: 794)

Segment cluster HSCP2_PEA_(—)1_node_(—)21 (SEQ ID NO: 823) according to the present invention is supported by 49 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HSCP2_PEA_(—)1_T4 (SEQ ID NO: 783), HSCP2_PEA_(—)1_T13 (SEQ ID NO: 784), HSCP2_PEA_(—)1_T19 (SEQ ID NO: 785), HSCP2_PEA_(—)1_T20 (SEQ ID NO: 786), HSCP2_PEA_(—)1_T22 (SEQ ID NO: 787), HSCP2_PEA_(—)1_T23 (SEQ ID NO: 788), HSCP2_PEA_(—)1_T25 (SEQ ID NO: 789), HSCP2_PEA_(—)1_T31 (SEQ ID NO: 790), HSCP2_PEA_(—)1_T33 (SEQ ID NO: 791), HSCP2_PEA_(—)1_T34 (SEQ ID NO: 792) and HSCP2_PEA_(—)1_T50 (SEQ ID NO: 794). Table 69 below describes the starting and ending position of this segment on each transcript.

TABLE 69 Segment location on transcripts Segment Segment Transcript starting ending name position position HSCP2_PEA_1_T4 1375 1457 (SEQ ID NO: 783) HSCP2_PEA_1_T13 1375 1457 (SEQ ID NO: 784) HSCP2_PEA_1_T19 1375 1457 (SEQ ID NO: 785) HSCP2_PEA_1_T20 1375 1457 (SEQ ID NO: 786) HSCP2_PEA_1_T22 1375 1457 (SEQ ID NO: 787) HSCP2_PEA_1_T23 1375 1457 (SEQ ID NO: 788) HSCP2_PEA_1_T25 1375 1457 (SEQ ID NO: 789) HSCP2_PEA_1_T31 988 1070 (SEQ ID NO: 790) HSCP2_PEA_1_T33 740 822 (SEQ ID NO: 791) HSCP2_PEA_1_T34 1375 1457 (SEQ ID NO: 792) HSCP2_PEA_1_T50 1375 1457 (SEQ ID NO: 794)

Segment cluster HSCP2_PEA_(—)1_node_(—)37 (SEQ ID NO: 824) according to the present invention is supported by 55 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HSCP2_PEA_(—)1_T4 (SEQ ID NO: 783), HSCP2_PEA_(—)1_T13 (SEQ ID NO: 784), HSCP2_PEA_(—)1_T19 (SEQ ID NO: 785), HSCP2_PEA_(—)1_T20 (SEQ ID NO: 786), HSCP2_PEA_(—)1_T22 (SEQ ID NO: 787), HSCP2_PEA_(—)1_T23 (SEQ ID NO: 788), HSCP2_PEA_(—)1_T25 (SEQ ID NO: 789), HSCP2_PEA_(—)1_T31 (SEQ ID NO: 790), HSCP2_PEA_(—)1_T33 (SEQ ID NO: 791) and HSCP2_PEA_(—)1_T50 (SEQ ID NO: 794). Table 70 below describes the starting and ending position of this segment on each transcript.

TABLE 70 Segment location on transcripts Segment Segment Transcript starting ending name position position HSCP2_PEA_1_T4 2327 2368 (SEQ ID NO: 783) HSCP2_PEA_1_T13 2327 2368 (SEQ ID NO: 784) HSCP2_PEA_1_T19 2114 2155 (SEQ ID NO: 785) HSCP2_PEA_1_T20 2327 2368 (SEQ ID NO: 786) HSCP2_PEA_1_T22 2327 2368 (SEQ ID NO: 787) HSCP2_PEA_1_T23 2327 2368 (SEQ ID NO: 788) HSCP2_PEA_1_T25 2327 2368 (SEQ ID NO: 789) HSCP2_PEA_1_T31 1940 1981 (SEQ ID NO: 790) HSCP2_PEA_1_T33 1692 1733 (SEQ ID NO: 791) HSCP2_PEA_1_T50 2327 2368 (SEQ ID NO: 794)

Segment cluster HSCP2_PEA_(—)1_node_(—)38 (SEQ ID NO: 825) according to the present invention is supported by 59 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HSCP2_PEA_(—)1_T4 (SEQ ID NO: 783), HSCP2_PEA_(—)1_T13 (SEQ ID NO: 784), HSCP2_PEA_(—)1_T19 (SEQ ID NO: 785), HSCP2_PEA_(—)1_T20 (SEQ ID NO: 786), HSCP2_PEA_(—)1_T22 (SEQ ID NO: 787), HSCP2_PEA_(—)1_T23 (SEQ ID NO: 788), HSCP2_PEA_(—)1_T25 (SEQ ID NO: 789), HSCP2_PEA_(—)1_T31 (SEQ ID NO: 790), HSCP2_PEA_(—)1_T33 (SEQ ID NO: 791) and HSCP2_PEA_(—)1_T50 (SEQ ID NO: 794). Table 71 below describes the starting and ending position of this segment on each transcript.

TABLE 71 Segment location on transcripts Segment Segment Transcript starting ending name position position HSCP2_PEA_1_T4 2369 2442 (SEQ ID NO: 783) HSCP2_PEA_1_T13 2369 2442 (SEQ ID NO: 784) HSCP2_PEA_1_T19 2156 2229 (SEQ ID NO: 785) HSCP2_PEA_1_T20 2369 2442 (SEQ ID NO: 786) HSCP2_PEA_1_T22 2369 2442 (SEQ ID NO: 787) HSCP2_PEA_1_T23 2369 2442 (SEQ ID NO: 788) HSCP2_PEA_1_T25 2369 2442 (SEQ ID NO: 789) HSCP2_PEA_1_T31 1982 2055 (SEQ ID NO: 790) HSCP2_PEA_1_T33 1734 1807 (SEQ ID NO: 791) HSCP2_PEA_1_T50 2369 2442 (SEQ ID NO: 794)

Segment cluster HSCP2_PEA_(—)1_node_(—)39 (SEQ ID NO: 826) according to the present invention is supported by 57 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HSCP2_PEA_(—)1_T4 (SEQ ID NO: 783), HSCP2_PEA_(—)1_T13 (SEQ ID NO: 784), HSCP2_PEA_(—)1_T19 (SEQ ID NO: 785), HSCP2_PEA_(—)1_T20 (SEQ ID NO: 786), HSCP2_PEA_(—)1_T22 (SEQ ID NO: 787), HSCP2_PEA_(—)1_T23 (SEQ ID NO: 788), HSCP2_PEA_(—)1_T25 (SEQ ID NO: 789), HSCP2_PEA_(—)1_T31 (SEQ ID NO: 790), HSCP2_PEA_(—)1_T33 (SEQ ID NO: 791) and HSCP2_PEA_(—)1_T50 (SEQ ID NO: 794). Table 72 below describes the starting and ending position of this segment on each transcript.

TABLE 72 Segment location on transcripts Segment Segment Transcript starting ending name position position HSCP2_PEA_1_T4 2443 2505 (SEQ ID NO: 783) HSCP2_PEA_1_T13 2443 2505 (SEQ ID NO: 784) HSCP2_PEA_1_T19 2230 2292 (SEQ ID NO: 785) HSCP2_PEA_1_T20 2443 2505 (SEQ ID NO: 786) HSCP2_PEA_1_T22 2443 2505 (SEQ ID NO: 787) HSCP2_PEA_1_T23 2443 2505 (SEQ ID NO: 788) HSCP2_PEA_1_T25 2443 2505 (SEQ ID NO: 789) HSCP2_PEA_1_T31 2056 2118 (SEQ ID NO: 790) HSCP2_PEA_1_T33 1808 1870 (SEQ ID NO: 791) HSCP2_PEA_1_T50 2443 2505 (SEQ ID NO: 794)

Segment cluster HSCP2_PEA_(—)1_node_(—)41 (SEQ ID NO: 827) according to the present invention is supported by 60 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HSCP2_PEA_(—)1_T4 (SEQ ID NO: 783), HSCP2_PEA_(—)1_T13 (SEQ ID NO: 784), HSCP2_PEA_(—)1_T19 (SEQ ID NO: 785), HSCP2_PEA_(—)1_T20 (SEQ ID NO: 786), HSCP2_PEA_(—)1_T22 (SEQ ID NO: 787), HSCP2_PEA_(—)1_T23 (SEQ ID NO: 788), HSCP2_PEA_(—)1_T25 (SEQ ID NO: 789), HSCP2_PEA_(—)1_T31 (SEQ ID NO: 790), HSCP2_PEA_(—)1_T33 (SEQ ID NO: 791) and HSCP2_PEA_(—)1_T50 (SEQ ID NO: 794). Table 73 below describes the starting and ending position of this segment on each transcript.

TABLE 73 Segment location on transcripts Segment Segment Transcript starting ending name position position HSCP2_PEA_1_T4 2506 2534 (SEQ ID NO: 783) HSCP2_PEA_1_T13 2506 2534 (SEQ ID NO: 784) HSCP2_PEA_1_T19 2293 2321 (SEQ ID NO: 785) HSCP2_PEA_1_T20 2506 2534 (SEQ ID NO: 786) HSCP2_PEA_1_T22 2506 2534 (SEQ ID NO: 787) HSCP2_PEA_1_T23 2506 2534 (SEQ ID NO: 788) HSCP2_PEA_1_T25 2506 2534 (SEQ ID NO: 789) HSCP2_PEA_1_T31 2119 2147 (SEQ ID NO: 790) HSCP2_PEA_1_T33 1871 1899 (SEQ ID NO: 791) HSCP2_PEA_1_T50 2506 2534 (SEQ ID NO: 794)

Segment cluster HSCP2_PEA_(—)1_node_(—)42 (SEQ ID NO: 828) according to the present invention is supported by 18 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HSCP2_PEA_(—)1_T22 (SEQ ID NO: 787). Table 74 below describes the starting and ending position of this segment on each transcript.

TABLE 74 Segment location on transcripts Segment Segment Transcript starting ending name position position HSCP2_PEA_1_T22 2535 2596 (SEQ ID NO: 787)

Segment cluster HSCP2_PEA_(—)1_node_(—)46 (SEQ ID NO: 829) according to the present invention can be found in the following transcript(s): HSCP2_PEA_(—)1_T4 (SEQ ID NO: 783), HSCP2_PEA_(—)1_T13 (SEQ ID NO: 784), HSCP2_PEA_(—)1_T19 (SEQ ID NO: 785), HSCP2_PEA_(—)1_T20 (SEQ ID NO: 786), HSCP2_PEA_(—)1_T22 (SEQ ID NO: 787), HSCP2_PEA_(—)1_T23 (SEQ ID NO: 788), HSCP2_PEA_(—)1_T25 (SEQ ID NO: 789), HSCP2_PEA_(—)1_T31 (SEQ ID NO: 790), HSCP2_PEA_(—)1_T33 (SEQ ID NO: 791) and HSCP2_PEA_(—)1_T50 (SEQ ID NO: 794). Table 75 below describes the starting and ending position of this segment on each transcript.

TABLE 75 Segment location on transcripts Segment Segment Transcript starting ending name position position HSCP2_PEA_1_T4 2535 2559 (SEQ ID NO: 783) HSCP2_PEA_1_T13 2535 2559 (SEQ ID NO: 784) HSCP2_PEA_1_T19 2322 2346 (SEQ ID NO: 785) HSCP2_PEA_1_T20 2535 2559 (SEQ ID NO: 786) HSCP2_PEA_1_T22 2597 2621 (SEQ ID NO: 787) HSCP2_PEA_1_T23 2535 2559 (SEQ ID NO: 788) HSCP2_PEA_1_T25 2535 2559 (SEQ ID NO: 789) HSCP2_PEA_1_T31 2148 2172 (SEQ ID NO: 790) HSCP2_PEA_1_T33 1900 1924 (SEQ ID NO: 791) HSCP2_PEA_1_T50 2535 2559 (SEQ ID NO: 794)

Segment cluster HSCP2_PEA_(—)1_node_(—)47 (SEQ ID NO: 830) according to the present invention is supported by 59 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HSCP2_PEA_(—)1_T4 (SEQ ID NO: 783), HSCP2_PEA_(—)1_T13 (SEQ ID NO: 784), HSCP2_PEA_(—)1_T19 (SEQ ID NO: 785), HSCP2_PEA_(—)1_T20 (SEQ ID NO: 786), HSCP2_PEA_(—)1_T22 (SEQ ID NO: 787), HSCP2_PEA_(—)1_T23 (SEQ ID NO: 788), HSCP2_PEA_(—)1_T25 (SEQ ID NO: 789), HSCP2_PEA_(—)1_T31 (SEQ ID NO: 790), HSCP2_PEA_(—)1_T33 (SEQ ID NO: 791) and HSCP2_PEA_(—)1_T50 (SEQ ID NO: 794). Table 76 below describes the starting and ending position of this segment on each transcript.

TABLE 76 Segment location on transcripts Segment Segment Transcript starting ending name position position HSCP2_PEA_1_T4 2560 2674 (SEQ ID NO: 783) HSCP2_PEA_1_T13 2560 2674 (SEQ ID NO: 784) HSCP2_PEA_1_T19 2347 2461 (SEQ ID NO: 785) HSCP2_PEA_1_T20 2560 2674 (SEQ ID NO: 786) HSCP2_PEA_1_T22 2622 2736 (SEQ ID NO: 787) HSCP2_PEA_1_T23 2560 2674 (SEQ ID NO: 788) HSCP2_PEA_1_T25 2560 2674 (SEQ ID NO: 789) HSCP2_PEA_1_T31 2173 2287 (SEQ ID NO: 790) HSCP2_PEA_1_T33 1925 2039 (SEQ ID NO: 791) HSCP2_PEA_1_T50 2560 2674 (SEQ ID NO: 794)

Segment cluster HSCP2_PEA_(—)1_node_(—)50 (SEQ ID NO: 831) according to the present invention is supported by 51 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HSCP2_PEA_(—)1_T4 (SEQ ID NO: 783), HSCP2_PEA_(—)1_T13 (SEQ ID NO: 784), HSCP2_PEA_(—)1_T19 (SEQ ID NO: 785), HSCP2_PEA_(—)1_T20 (SEQ ID NO: 786), HSCP2_PEA_(—)1_T22 (SEQ ID NO: 787), HSCP2_PEA_(—)1_T23 (SEQ ID NO: 788), HSCP2_PEA_(—)1_T25 (SEQ ID NO: 789), HSCP2_PEA_(—)1_T31 (SEQ ID NO: 790), HSCP2_PEA_(—)1_T33 (SEQ ID NO: 791) and HSCP2_PEA_(—)1_T50 (SEQ ID NO: 794). Table 77 below describes the starting and ending position of this segment on each transcript.

TABLE 77 Segment location on transcripts Segment Segment Transcript starting ending name position position HSCP2_PEA_1_T4 2675 2731 (SEQ ID NO: 783) HSCP2_PEA_1_T13 2675 2731 (SEQ ID NO: 784) HSCP2_PEA_1_T19 2462 2518 (SEQ ID NO: 785) HSCP2_PEA_1_T20 2675 2731 (SEQ ID NO: 786) HSCP2_PEA_1_T22 2737 2793 (SEQ ID NO: 787) HSCP2_PEA_1_T23 2675 2731 (SEQ ID NO: 788) HSCP2_PEA_1_T25 2675 2731 (SEQ ID NO: 789) HSCP2_PEA_1_T31 2288 2344 (SEQ ID NO: 790) HSCP2_PEA_1_T33 2040 2096 (SEQ ID NO: 791) HSCP2_PEA_1_T50 2675 2731 (SEQ ID NO: 794)

Segment cluster HSCP2_PEA_(—)1_node_(—)51 (SEQ ID NO: 832) according to the present invention is supported by 58 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HSCP2_PEA_(—)1_T4 (SEQ ID NO: 783), HSCP2_PEA_(—)1_T13 (SEQ ID NO: 784), HSCP2_PEA_(—)1_T19 (SEQ ID NO: 785), HSCP2_PEA_(—)1_T20 (SEQ ID NO: 786), HSCP2_PEA_(—)1_T22 (SEQ ID NO: 787), HSCP2_PEA_(—)1_T23 (SEQ ID NO: 788), HSCP2_PEA_(—)1_T25 (SEQ ID NO: 789), HSCP2_PEA_(—)1_T31 (SEQ ID NO: 790), HSCP2_PEA_(—)1_T33 (SEQ ID NO: 791) and HSCP2_PEA_(—)1_T50 (SEQ ID NO: 794). Table 78 below describes the starting and ending position of this segment on each transcript.

TABLE 78 Segment location on transcripts Segment Segment Transcript starting ending name position position HSCP2_PEA_1_T4 2732 2803 (SEQ ID NO: 783) HSCP2_PEA_1_T13 2732 2803 (SEQ ID NO: 784) HSCP2_PEA_1_T19 2519 2590 (SEQ ID NO: 785) HSCP2_PEA_1_T20 2732 2803 (SEQ ID NO: 786) HSCP2_PEA_1_T22 2794 2865 (SEQ ID NO: 787) HSCP2_PEA_1_T23 2732 2803 (SEQ ID NO: 788) HSCP2_PEA_1_T25 2732 2803 (SEQ ID NO: 789) HSCP2_PEA_1_T31 2345 2416 (SEQ ID NO: 790) HSCP2_PEA_1_T33 2097 2168 (SEQ ID NO: 791) HSCP2_PEA_1_T50 2732 2803 (SEQ ID NO: 794)

Segment cluster HSCP2_PEA_(—)1_node_(—)55 (SEQ ID NO: 833) according to the present invention is supported by 65 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HSCP2_PEA_(—)1_T4 (SEQ ID NO: 783), HSCP2_PEA_(—)1_T13 (SEQ ID NO: 784), HSCP2_PEA_(—)1_T19 (SEQ ID NO: 785), HSCP2_PEA_(—)1_T20 (SEQ ID NO: 786), HSCP2_PEA_(—)1_T23 (SEQ ID NO: 788), HSCP2_PEA_(—)1_T25 (SEQ ID NO: 789), HSCP2_PEA_(—)1_T31 (SEQ ID NO: 790), HSCP2_PEA_(—)1_T33 (SEQ ID NO: 791) and HSCP2_PEA_(—)1_T50 (SEQ ID NO: 794). Table 79 below describes the starting and ending position of this segment on each transcript.

TABLE 79 Segment location on transcripts Segment Segment Transcript starting ending name position position HSCP2_PEA_1_T4 2804 2880 (SEQ ID NO: 783) HSCP2_PEA_1_T13 2804 2880 (SEQ ID NO: 784) HSCP2_PEA_1_T19 2591 2667 (SEQ ID NO: 785) HSCP2_PEA_1_T20 2804 2880 (SEQ ID NO: 786) HSCP2_PEA_1_T23 2804 2880 (SEQ ID NO: 788) HSCP2_PEA_1_T25 2804 2880 (SEQ ID NO: 789) HSCP2_PEA_1_T31 2417 2493 (SEQ ID NO: 790) HSCP2_PEA_1_T33 2169 2245 (SEQ ID NO: 791) HSCP2_PEA_1_T50 2804 2880 (SEQ ID NO: 794)

Segment cluster HSCP2_PEA_(—)1_node_(—)56 (SEQ ID NO: 834) according to the present invention is supported by 58 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HSCP2_PEA_(—)1_T4 (SEQ ID NO: 783), HSCP2_PEA_(—)1_T13 (SEQ ID NO: 784), HSCP2_PEA_(—)1_T19 (SEQ ID NO: 785), HSCP2_PEA_(—)1_T20 (SEQ ID NO: 786), HSCP2_PEA_(—)1_T23 (SEQ ID NO: 788), HSCP2_PEA_(—)1_T25 (SEQ ID NO: 789), HSCP2_PEA_(—)1_T31 (SEQ ID NO: 790), HSCP2_PEA_(—)1_T33 (SEQ ID NO: 791) and HSCP2_PEA_(—)1_T50 (SEQ ID NO: 794). Table 80 below describes the starting and ending position of this segment on each transcript.

TABLE 80 Segment location on transcripts Segment Segment Transcript starting ending name position position HSCP2_PEA_1_T4 2881 2910 (SEQ ID NO: 783) HSCP2_PEA_1_T13 2881 2910 (SEQ ID NO: 784) HSCP2_PEA_1_T19 2668 2697 (SEQ ID NO: 785) HSCP2_PEA_1_T20 2881 2910 (SEQ ID NO: 786) HSCP2_PEA_1_T23 2881 2910 (SEQ ID NO: 788) HSCP2_PEA_1_T25 2881 2910 (SEQ ID NO: 789) HSCP2_PEA_1_T31 2494 2523 (SEQ ID NO: 790) HSCP2_PEA_1_T33 2246 2275 (SEQ ID NO: 791) HSCP2_PEA_1_T50 2881 2910 (SEQ ID NO: 794)

Segment cluster HSCP2_PEA_(—)1_node_(—)60 (SEQ ID NO: 835) according to the present invention is supported by 90 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HSCP2_PEA_(—)1_T4 (SEQ ID NO: 783), HSCP2_PEA_(—)1_T13 (SEQ ID NO: 784), HSCP2_PEA_(—)1_T19 (SEQ ID NO: 785), HSCP2_PEA_(—)1_T20 (SEQ ID NO: 786), HSCP2_PEA_(—)1_T23 (SEQ ID NO: 788), HSCP2_PEA_(—)1_T25 (SEQ ID NO: 789), HSCP2_PEA_(—)1_T31 (SEQ ID NO: 790), HSCP2_PEA_(—)1_T33 (SEQ ID NO: 791) and HSCP2_PEA_(—)1_T50 (SEQ ID NO: 794). Table 81 below describes the starting and ending position of this segment on each transcript.

TABLE 81 Segment location on transcripts Segment Segment Transcript name starting position ending position HSCP2_PEA_1_T4 3128 3234 (SEQ ID NO: 783) HSCP2_PEA_1_T13 3128 3234 (SEQ ID NO: 784) HSCP2_PEA_1_T19 2915 3021 (SEQ ID NO: 785) HSCP2_PEA_1_T20 3128 3234 (SEQ ID NO: 786) HSCP2_PEA_1_T23 3128 3234 (SEQ ID NO: 788) HSCP2_PEA_1_T25 3128 3234 (SEQ ID NO: 789) HSCP2_PEA_1_T31 2741 2847 (SEQ ID NO: 790) HSCP2_PEA_1_T33 2493 2599 (SEQ ID NO: 791) HSCP2_PEA_1_T50 3128 3234 (SEQ ID NO: 794)

Segment cluster HSCP2_PEA_(—)1_node_(—)61 (SEQ ID NO: 836) according to the present invention is supported by 81 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HSCP2_PEA_(—)1_T4 (SEQ ID NO: 783), HSCP2_PEA_(—)1_T13 (SEQ ID NO: 784), HSCP2_PEA_(—)1_T19 (SEQ ID NO: 785), HSCP2_PEA_(—)1_T20 (SEQ ID NO: 786), HSCP2_PEA_(—)1_T23 (SEQ ID NO: 788), HSCP2_PEA_(—)1_T25 (SEQ ID NO: 789), HSCP2_PEA_(—)1_T31 (SEQ ID NO: 790), HSCP2_PEA_(—)1_T33 (SEQ ID NO: 791) and HSCP2_PEA_(—)1_T50 (SEQ ID NO: 794). Table 82 below describes the starting and ending position of this segment on each transcript.

TABLE 82 Segment location on transcripts Segment Segment Transcript name starting position ending position HSCP2_PEA_1_T4 3235 3267 (SEQ ID NO: 783) HSCP2_PEA_1_T13 3235 3267 (SEQ ID NO: 784) HSCP2_PEA_1_T19 3022 3054 (SEQ ID NO: 785) HSCP2_PEA_1_T20 3235 3267 (SEQ ID NO: 786) HSCP2_PEA_1_T23 3235 3267 (SEQ ID NO: 788) HSCP2_PEA_1_T25 3235 3267 (SEQ ID NO: 789) HSCP2_PEA_1_T31 2848 2880 (SEQ ID NO: 790) HSCP2_PEA_1_T33 2600 2632 (SEQ ID NO: 791) HSCP2_PEA_1_T50 3235 3267 (SEQ ID NO: 794)

Segment cluster HSCP2_PEA_(—)1_node_(—)67 (SEQ ID NO: 837) according to the present invention can be found in the following transcript(s): HSCP2_PEA_(—)1_T4 (SEQ ID NO: 783), HSCP2_PEA_(—)1_T19 (SEQ ID NO: 785), HSCP2_PEA_(—)1_T20 (SEQ ID NO: 786), HSCP2_PEA_(—)1_T31 (SEQ ID NO: 790), HSCP2_PEA_(—)1_T33 (SEQ ID NO: 791) and HSCP2_PEA_(—)1_T50 (SEQ ID NO: 794). Table 83 below describes the starting and ending position of this segment on each transcript.

TABLE 83 Segment location on transcripts Segment Segment Transcript name starting position ending position HSCP2_PEA_1_T4 3268 3272 (SEQ ID NO: 783) HSCP2_PEA_1_T19 3055 3059 (SEQ ID NO: 785) HSCP2_PEA_1_T20 3268 3272 (SEQ ID NO: 786) HSCP2_PEA_1_T31 2881 2885 (SEQ ID NO: 790) HSCP2_PEA_1_T33 2633 2637 (SEQ ID NO: 791) HSCP2_PEA_1_T50 3268 3272 (SEQ ID NO: 794)

Segment cluster HSCP2_PEA_(—)1_node_(—)68 (SEQ ID NO: 838) according to the present invention is supported by 88 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HSCP2_PEA_(—)1_T4 (SEQ ID NO: 783), HSCP2_PEA_(—)1_T19 (SEQ ID NO: 785), HSCP2_PEA_(—)1_T20 (SEQ ID NO: 786), HSCP2_PEA_(—)1_T25 (SEQ ID NO: 789), HSCP2_PEA_(—)1_T31 (SEQ ID NO: 790), HSCP2_PEA_(—)1_T33 (SEQ ID NO: 791) and HSCP2_PEA_(—)1_T50 (SEQ ID NO: 794). Table 84 below describes the starting and ending position of this segment on each transcript.

TABLE 84 Segment location on transcripts Segment Segment Transcript name starting position ending position HSCP2_PEA_1_T4 3273 3328 (SEQ ID NO: 783) HSCP2_PEA_1_T19 3060 3115 (SEQ ID NO: 785) HSCP2_PEA_1_T20 3273 3328 (SEQ ID NO: 786) HSCP2_PEA_1_T25 3268 3323 (SEQ ID NO: 789) HSCP2_PEA_1_T31 2886 2941 (SEQ ID NO: 790) HSCP2_PEA_1_T33 2638 2693 (SEQ ID NO: 791) HSCP2_PEA_1_T50 3273 3328 (SEQ ID NO: 794)

Segment cluster HSCP2_PEA_(—)1_node_(—)69 (SEQ ID NO: 839) according to the present invention is supported by 96 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HSCP2_PEA_(—)1_T4 (SEQ ID NO: 783), HSCP2_PEA_(—)1_T13 (SEQ ID NO: 784), HSCP2_PEA_(—)1_T19 (SEQ ID NO: 785), HSCP2_PEA_(—)1_T20 (SEQ ID NO: 786), HSCP2_PEA_(—)1_T25 (SEQ ID NO: 789), HSCP2_PEA_(—)1_T31 (SEQ ID NO: 790), HSCP2_PEA_(—)1_T33 (SEQ ID NO: 791) and HSCP2_PEA_(—)1_T50 (SEQ ID NO: 794). Table 85 below describes the starting and ending position of this segment on each transcript.

TABLE 85 Segment location on transcripts Segment Segment Transcript name starting position ending position HSCP2_PEA_1_T4 3329 3430 (SEQ ID NO: 783) HSCP2_PEA_1_T13 3268 3369 (SEQ ID NO: 784) HSCP2_PEA_1_T19 3116 3217 (SEQ ID NO: 785) HSCP2_PEA_1_T20 3329 3430 (SEQ ID NO: 786) HSCP2_PEA_1_T25 3324 3425 (SEQ ID NO: 789) HSCP2_PEA_1_T31 2942 3043 (SEQ ID NO: 790) HSCP2_PEA_1_T33 2694 2795 (SEQ ID NO: 791) HSCP2_PEA_1_T50 3329 3430 (SEQ ID NO: 794)

Segment cluster HSCP2_PEA_(—)1_node_(—)70 (SEQ ID NO: 840) according to the present invention can be found in the following transcript(s): HSCP2_PEA_(—)1_T20 (SEQ ID NO: 786). Table 86 below describes the starting and ending position of this segment on each transcript.

TABLE 86 Segment location on transcripts Segment Segment Transcript name starting position ending position HSCP2_PEA_1_T20 3431 3442 (SEQ ID NO: 786)

Segment cluster HSCP2_PEA_(—)1_node_(—)75 (SEQ ID NO: 841) according to the present invention is supported by 46 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HSCP2_PEA_(—)1_T4 (SEQ ID NO: 783), HSCP2_PEA_(—)1_T13 (SEQ ID NO: 784), HSCP2_PEA_(—)1_T19 (SEQ ID NO: 785), HSCP2_PEA_(—)1_T31 (SEQ ID NO: 790) and HSCP2_PEA_(—)1_T33 (SEQ ID NO: 791). Table 87 below describes the starting and ending position of this segment on each transcript.

TABLE 87 Segment location on transcripts Segment Segment Transcript name starting position ending position HSCP2_PEA_1_T4 5883 5935 (SEQ ID NO: 783) HSCP2_PEA_1_T13 3672 3724 (SEQ ID NO: 784) HSCP2_PEA_1_T19 3520 3572 (SEQ ID NO: 785) HSCP2_PEA_1_T31 3346 3398 (SEQ ID NO: 790) HSCP2_PEA_1_T33 3098 3150 (SEQ ID NO: 791)

Segment cluster HSCP2_PEA_(—)1_node_(—)77 (SEQ ID NO: 842) according to the present invention is supported by 47 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HSCP2_PEA_(—)1_T4 (SEQ ID NO: 783), HSCP2_PEA_(—)1_T13 (SEQ ID NO: 784), HSCP2_PEA_(—)1_T19 (SEQ ID NO: 785), HSCP2_PEA_(—)1_T31 (SEQ ID NO: 790) and HSCP2_PEA_(—)1_T33 (SEQ ID NO: 791). Table 88 below describes the starting and ending position of this segment on each transcript.

TABLE 88 Segment location on transcripts Segment Segment Transcript name starting position ending position HSCP2_PEA_1_T4 6216 6269 (SEQ ID NO: 783) HSCP2_PEA_1_T13 4005 4058 (SEQ ID NO: 784) HSCP2_PEA_1_T19 3853 3906 (SEQ ID NO: 785) HSCP2_PEA_1_T31 3679 3732 (SEQ ID NO: 790) HSCP2_PEA_1_T33 3431 3484 (SEQ ID NO: 791)

Segment cluster HSCP2_PEA_(—)1_node_(—)79 (SEQ ID NO: 843) according to the present invention is supported by 55 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HSCP2_PEA_(—)1_T4 (SEQ ID NO: 783), HSCP2_PEA_(—)1_T13 (SEQ ID NO: 784), HSCP2_PEA_(—)1_T19 (SEQ ID NO: 785), HSCP2_PEA_(—)1_T31 (SEQ ID NO: 790) and HSCP2_PEA_(—)1_T33 (SEQ ID NO: 791). Table 89 below describes the starting and ending position of this segment on each transcript.

TABLE 89 Segment location on transcripts Segment Segment Transcript name starting position ending position HSCP2_PEA_1_T4 6495 6548 (SEQ ID NO: 783) HSCP2_PEA_1_T13 4284 4337 (SEQ ID NO: 784) HSCP2_PEA_1_T19 4132 4185 (SEQ ID NO: 785) HSCP2_PEA_1_T31 3958 4011 (SEQ ID NO: 790) HSCP2_PEA_1_T33 3710 3763 (SEQ ID NO: 791)

Segment cluster HSCP2_PEA_(—)1_node_(—)82 (SEQ ID NO: 844) according to the present invention is supported by 38 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HSCP2_PEA_(—)1_T20 (SEQ ID NO: 786) and HSCP2_PEA_(—)1_T23 (SEQ ID NO: 788). Table 90 below describes the starting and ending position of this segment on each transcript.

TABLE 90 Segment location on transcripts Segment Segment Transcript name starting position ending position HSCP2_PEA_1_T20 3443 3547 (SEQ ID NO: 786) HSCP2_PEA_1_T23 3268 3372 (SEQ ID NO: 788) Variant protein alignment to the previously known protein: Sequence name: CERU_HUMAN Sequence documentation:

Alignment of: HSCP2_PEA_(—)1_P4 (SEQ ID NO: 846) x CERU_HUMAN ..

Alignment segment 1/1:

Quality: 10630.00 Escore: 0 Matching length: 1060 Total length: 1060 Matching Percent Similarity: 100.00 Matching Percent Identity: 100.00 Total Percent Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0

Alignment:

Sequence name: CERU_HUMAN Sequence documentation:

Alignment of: HSCP2_PEA_(—)1_P8 (SEQ ID NO: 847) x CERU_HUMAN ..

Alignment segment 1/1:

Quality: 10079.00 Escore: 0 Matching length: 1006 Total length: 1006 Matching Percent Similarity: 100.00 Matching Percent Identity: 100.00 Total Percent Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0

Alignment:

Sequence name: CERU_HUMAN Sequence documentation:

Alignment of: HSCP2_PEA_(—)1_P14 (SEQ ID NO: 848) x CERU_HUMAN ..

Alignment segment 1/1:

Quality: 9832.00 Escore: 0 Matching length: 994 Total length: 1065 Matching Percent Similarity: 99.90 Matching Percent Identity: 99.90 Total Percent Similarity: 93.24 Total Percent Identity: 93.24 Gaps: 1

Alignment:

Sequence name: CERU_HUMAN Sequence documentation:

Alignment of: HSCP2_PEA_(—)1_P15 (SEQ ID NO: 849) x CERU_HUMAN ..

Alignment segment 1/1:

Quality: 10630.00 Escore: 0 Matching length: 1060 Total length: 1060 Matching Percent Similarity: 100.00 Matching Percent Identity: 100.00 Total Percent Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0

Alignment:

Sequence name: CERU_HUMAN Sequence documentation:

Alignment of: HSCP2_PEA_(—)1_P2 (SEQ ID NO: 850) x CERU_HUMAN ..

Alignment segment 1/1:

Quality: 7636.00 Escore: 0 Matching length: 761 Total length: 761 Matching Percent Similarity: 100.00 Matching Percent Identity: 100.00 Total Percent Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0

Alignment:

Sequence name: CERU_HUMAN Sequence documentation:

Alignment of: HSCP2_PEA_(—)1_P16 (SEQ ID NO: 851) x CERU_HUMAN ..

Alignment segment 1/1:

Quality: 10092.00 Escore: 0 Matching length: 1007 Total length: 1007 Matching Percent Similarity: 100.00 Matching Percent Identity: 100.00 Total Percent Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0

Alignment:

Sequence name: CERU_HUMAN Sequence documentation:

Alignment of: HSCP2_PEA_(—)1_P6 (SEQ ID NO: 852) x CERU_HUMAN ..

Alignment segment 1/1:.

Quality: 10079.00 Escore: 0 Matching length: 1006 Total length: 1006 Matching Percent Similarity: 100.00 Matching Percent Identity: 100.00 Total Percent Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0

Alignment:

Sequence name: CERU_HUMAN Sequence documentation:

Alignment of: HSCP2_PEA_(—)1_P2 (SEQ ID NO: 850)2 x CERU_HUMAN ..

Alignment segment 1/1:

Quality: 9277.00 Escore: 0 Matching length: 936 Total length: 1065 Matching Percent Similarity: 100.00 Matching Percent Identity: 99.89 Total Percent Similarity: 87.89 Total Percent Identity: 87.79 Gaps: 1

Alignment:

Sequence name: CERU_HUMAN Sequence documentation:

Alignment of: HSCP2_PEA_(—)1_P2 (SEQ ID NO: 850)4 x CERU_HUMAN ..

Alignment segment 1/1:

Quality: 8074.00 Escore: 0 Matching length: 804 Total length: 804 Matching Percent Similarity: 100.00 Matching Percent Identity: 100.00 Total Percent Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0

Alignment:

Sequence name: CERU_HUMAN Sequence documentation:

Alignment of: HSCP2_PEA_(—)1_P2 (SEQ ID NO: 850)5 x CERU_HUMAN ..

Alignment segment 1/1:

Quality: 6196.00 Escore: 0 Matching length: 621 Total length: 621 Matching Percent Similarity: 100.00 Matching Percent Identity: 100.00 Total Percent Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0

Alignment:

Sequence name: CERU_HUMAN Sequence documentation:

Alignment of: HSCP2_PEA_(—)1_P33 (SEQ ID NO: 856) x CERU_HUMAN ..

Alignment segment 1/1:

Quality: 2003.00 Escore: 0 Matching length: 202 Total length: 202 Matching Percent Similarity: 100.00 Matching Percent Identity: 100.00 Total Percent Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0

Alignment:

Description for Cluster HUMTEN

Cluster HUMTEN features 19 transcript(s) and 57 segment(s) of interest, the names for which are given in Tables 1 and 2, respectively, the sequences themselves are given at the end of the application. The selected protein variants are given in table 3.

TABLE 1 Transcripts of interest SEQ ID Transcript Name NO: HUMTEN_PEA_1_T4 857 HUMTEN_PEA_1_T5 858 HUMTEN_PEA_1_T6 859 HUMTEN_PEA_1_T7 860 HUMTEN_PEA_1_T11 861 HUMTEN_PEA_1_T14 862 HUMTEN_PEA_1_T16 863 HUMTEN_PEA_1_T17 864 HUMTEN_PEA_1_T18 865 HUMTEN_PEA_1_T19 866 HUMTEN_PEA_1_T20 867 HUMTEN_PEA_1_T23 868 HUMTEN_PEA_1_T32 869 HUMTEN_PEA_1_T35 870 HUMTEN_PEA_1_T36 871 HUMTEN_PEA_1_T37 872 HUMTEN_PEA_1_T39 873 HUMTEN_PEA_1_T40 874 HUMTEN_PEA_1_T41 875

TABLE 2 Segments of interest SEQ ID Segment Name NO: HUMTEN_PEA_1_node_0 876 HUMTEN_PEA_1_node_2 877 HUMTEN_PEA_1_node_5 878 HUMTEN_PEA_1_node_6 879 HUMTEN_PEA_1_node_11 880 HUMTEN_PEA_1_node_12 881 HUMTEN_PEA_1_node_16 882 HUMTEN_PEA_1_node_19 883 HUMTEN_PEA_1_node_23 884 HUMTEN_PEA_1_node_27 885 HUMTEN_PEA_1_node_28 886 HUMTEN_PEA_1_node_30 887 HUMTEN_PEA_1_node_32 888 HUMTEN_PEA_1_node_33 889 HUMTEN_PEA_1_node_35 890 HUMTEN_PEA_1_node_38 891 HUMTEN_PEA_1_node_40 892 HUMTEN_PEA_1_node_42 893 HUMTEN_PEA_1_node_43 894 HUMTEN_PEA_1_node_44 895 HUMTEN_PEA_1_node_45 896 HUMTEN_PEA_1_node_46 897 HUMTEN_PEA_1_node_47 898 HUMTEN_PEA_1_node_49 899 HUMTEN_PEA_1_node_51 900 HUMTEN_PEA_1_node_56 901 HUMTEN_PEA_1_node_65 902 HUMTEN_PEA_1_node_71 903 HUMTEN_PEA_1_node_73 904 HUMTEN_PEA_1_node_76 905 HUMTEN_PEA_1_node_79 906 HUMTEN_PEA_1_node_83 907 HUMTEN_PEA_1_node_89 908 HUMTEN_PEA_1_node_7 909 HUMTEN_PEA_1_node_8 910 HUMTEN_PEA_1_node_9 911 HUMTEN_PEA_1_node_14 912 HUMTEN_PEA_1_node_17 913 HUMTEN_PEA_1_node_21 914 HUMTEN_PEA_1_node_22 915 HUMTEN_PEA_1_node_25 916 HUMTEN_PEA_1_node_36 917 HUMTEN_PEA_1_node_53 918 HUMTEN_PEA_1_node_54 919 HUMTEN_PEA_1_node_57 920 HUMTEN_PEA_1_node_61 921 HUMTEN_PEA_1_node_62 922 HUMTEN_PEA_1_node_67 923 HUMTEN_PEA_1_node_68 924 HUMTEN_PEA_1_node_69 925 HUMTEN_PEA_1_node_70 926 HUMIEN_PEA_1_node_72 927 HUMTEN_PEA_1_node_84 928 HUMTEN_PEA_1_node_85 929 HUMTEN_PEA_1_node_86 930 HUMTEN_PEA_1_node_87 931 HUMTEN_PEA_1_node_88 932

TABLE 3 Proteins of interest SEQ ID Corresponding Protein Name NO: Transcript(s) HUMTEN_PEA_1_P5 934 HUMTEN_PEA_1_T4 (SEQ ID NO:857) HUMTEN_PEA_1_P6 935 HUMTEN_PEA_1_T5 (SEQ ID NO:858) HUMTEN_PEA_1_P7 936 HUMTEN_PEA_1_T6 (SEQ ID NO:859) HUMTEN_PEA_1_P8 937 HUMTEN_PEA_1_T7 (SEQ ID NO:860) HUMTEN_PEA_1_P10 938 HUMTEN_PEA_1_T11 (SEQ ID NO:861) HUMTEN_PEA_1_P11 939 HUMTEN_PEA_1_T14 (SEQ ID NO:862) HUMTEN_PEA_1_P13 940 HUMTEN_PEA_1_T16 (SEQ ID NO:863) HUMTEN_PEA_1_P14 941 HUMTEN_PEA_1_T17 (SEQ ID NO:864) HUMTEN_PEA_1_P15 942 HUMTEN_PEA_1_T18 (SEQ ID NO:865) HUMTEN_PEA_1_P16 943 HUMTEN_PEA_1_T19 (SEQ ID NO:866) HUMTEN_PEA_1_P17 944 HUMTEN_PEA_1_T20 (SEQ ID NO.867) HUMTEN_PEA_1_P20 945 HUMTEN_PEA_1_T23 (SEQ ID NO:868) HUMTEN_PEA_1_P26 946 HUMTEN_PEA_1_T32 (SEQ ID NO:869) HUMTEN_PEA_1_P27 947 HUMTEN_PEA_1_T35 (SEQ ID NO:870) HUMTEN_PEA_1_P28 948 HUMTEN_PEA_1_T36 (SEQ ID NO:871) HUMTEN_PEA_1_P29 949 HUMTEN_PEA_1_T37 (SEQ ID NO:872) HUMTEN_PEA_1_P30 950 HUMTEN_PEA_1_T39 (SEQ ID NO:873) HUMTEN_PEA_1_P31 951 HUMTEN_PEA_1_T40 (SEQ ID NO:874) HUMTEN_PEA_1_P32 952 HUMTEN_PEA_1_T41 (SEQ ID NO:875)

These sequences are variants of the known protein Tenascin precursor (SwissProt accession identifier TENA_HUMAN; known also according to the synonyms TN; Hexabrachion; Cytotactin; Neuronectin; GMEM; JI; Miotendinous antigen; Glioma-associated-extracellular matrix antigen; GP 150-225; Tenascin-C; TN-C), SEQ ID NO: 933, referred to herein as the previously known protein.

Protein Tenascin precursor is known or believed to have the following function(s): SAM (substrate-adhesion molecule) that appears to inhibit cell migration. May play a role in supporting the growth of epithelial tumors. Is a ligand for integrins alpha-8/beta-1, alpha-9/beta-1, alpha-v/beta-3 and alpha-v/beta-6. The sequence for protein Tenascin precursor is given at the end of the application, as “Tenascin precursor amino acid sequence”. Known polymorphisms for this sequence are as shown in Table 4.

TABLE 4 Amino acid mutations for Known Protein SNP position(s) on amino acid sequence Comment 2008 Q -> E (in dbSNP: 13321). /FTId = VAR_014665.  244 Missing  370 L -> V  539 Q -> R  680 Q -> R 1066 R -> H 1600-1608 SGFTQGHQT -> LWLHPRASN 1677 L -> I 2054 F -> FLH 2055 W -> L 2140-2143 YKGA -> TRG

Protein Tenascin precursor localization is believed to be secreted; extracellular matrix.

It has been investigated for clinical/therapeutic use in humans, for example as a target for an antibody or small molecule, and/or as a direct therapeutic; available information related to these investigations is as follows. Potential pharmaceutically related or therapeutically related activity or activities of the previously known protein are as follows: DNA antagonist. A therapeutic role for a protein represented by the cluster has been predicted. The cluster was assigned this field because there was information in the drug database or the public databases (e.g., described herein above) that this protein, or part thereof, is used or can be used for a potential therapeutic indication: Anticancer; antibody.

The following GO Annotation(s) apply to the previously known protein. The following annotation(s) were found: cell adhesion, which are annotation(s) related to Biological Process; cell adhesion receptor; ligand binding or carrier; protein binding, which are annotation(s) related to Molecular Function; and extracellular matrix, which are annotation(s) related to Cellular Component.

The GO assignment relies on information from one or more of the SwissProt/TremBl Protein knowledgebase, available from <http://www.expasy.ch/sprot/>; or Locuslink, available from <http://www.ncbi.nlm.nih.gov/projects/LocusLink/>.

Cluster HUMTEN can be used as a diagnostic marker according to overexpression of transcripts of this cluster in cancer. Expression of such transcripts in normal tissues is also given according to the previously described methods. The term “number” in the left hand column of the table and the numbers on the y-axis of FIG. 37 refer to weighted expression of ESTs in each category, as “parts per million” (ratio of the expression of ESTs for a particular cluster to the expression of all ESTs in that category, according to parts per million).

Overall, the following results were obtained as shown with regard to the histograms in FIG. 37 and Table 5. This cluster is overexpressed (at least at a minimum level) in the following pathological conditions: a mixture of malignant tumors from different tissues, ovarian carcinoma, pancreas carcinoma and skin malignancies.

TABLE 5 Normal tissue distribution Name of Tissue Number adrenal 0 bladder 82 bone 867 brain 41 colon 154 epithelial 87 general 83 head and neck 20 kidney 123 lung 97 lymph nodes 37 breast 96 muscle 7 ovary 0 pancreas 10 prostate 38 skin 32 stomach 146 Thyroid 0 uterus 195

TABLE 6 P values and ratios for expression in cancerous tissue Name of Tissue P1 P2 SP1 R3 SP2 R4 adrenal 4.2e−01 4.6e−01 2.1e−01 3.4 2.9e−01 2.7 bladder 2.8e−01 4.2e−01 3.5e−01 1.6 6.0e−01 1.1 bone 4.7e−01 7.4e−01 3.2e−01 0.3 9.8e−01 0.4 brain 5.5e−02 8.0e−02 1.7e−06 2.3 5.1e−04 1.5 colon 6.5e−01 7.6e−01 9.4e−01 0.5 9.8e−01 0.4 epithelial 2.4e−02 4.2e−01 4.2e−03 1.3 7.5e−01 0.8 general 8.7e−05 3.2e−02 1.8e−09 1.7 2.1e−02 1.1 head and neck 2.3e−01 4.0e−01 9.9e−02 3.5 4.2e−01 1.6 kidney 7.0e−01 8.2e−01 6.2e−01 1.0 8.8e−01 0.6 lung 5.1e−01 6.5e−01 1.5e−01 1.5 3.2e−01 1.1 lymph nodes 3.3e−01 7.6e−01 3.2e−01 2.0 7.9e−01 0.8 breast 1.0e−01 2.3e−01 1.4e−01 1.6 5.3e−01 1.0 muscle 4.0e−02 1.7e−02 1.5e−01 5.6 1.5e−01 3.2 ovary 1.4e−01 1.7e−01 7.0e−04 3.4 6.4e−03 2.6 pancreas 7.5e−02 2.0e−01 5.8e−03 5.3 2.8e−02 3.6 prostate 8.4e−01 8.6e−01 3.6e−01 1.2 4.4e−01 1.1 skin 2.8e−01 1.7e−01 3.2e−05 5.6 5.5e−02 1.8 stomach 5.8e−01 7.5e−01 1 0.2 1 0.3 Thyroid 3.6e−01 3.6e−01 1 1.2 1 1.2 uterus 2.9e−01 7.4e−01 8.0e−01 0.6 9.9e−01 0.4

As noted above, cluster HUMTEN features 19 transcript(s), which were listed in Table 1 above. These transcript(s) encode for protein(s) which are variant(s) of protein Tenascin precursor. A description of each variant protein according to the present invention is now provided.

Variant protein HUMTEN_PEA_(—)1_P5 (SEQ ID NO: 934) according to the present invention has an amino acid sequence as given at the end of the application; it is encoded by transcript(s) HUMTEN_PEA_(—)1_T4 (SEQ ID NO: 857). An alignment is given to the known protein (Tenascin precursor) at the end of the application. One or more alignments to one or more previously published protein sequences are given at the end of the application. A brief description of the relationship of the variant protein according to the present invention to each such aligned protein is as follows:

Comparison report between HUMTEN_PEA_(—)1_P5 (SEQ ID NO: 934) and TENA_HUMAN_V1:

1. An isolated chimeric polypeptide encoding for HUMTEN_PEA_(—)1_P5 (SEQ ID NO: 934), comprising a first amino acid sequence being at least 90% homologous to MGAMTQLLAGVFLAFLALATEGGVLKKVIRHKRQSGVNATLPEENQPVVFNHVYNIK LPVGSQCSVDLESASGEKDLAPPSEPSESFQEHTVDGENQIVFTHRINIPRRACGCAAAP DVKELLSRLEELENLVSSLREQCTAGAGCCLQPATGRLDTRPFCSGRGNFSTEGCGCVC EPGWKGPNCSEPECPGNCHLRGRCIDGQCICDDGFTGEDCSQLACPSDCNDQGKCVNG VCICFEGYAGADCSREICPVPCSEEHGTCVDGLCVCHDGFAGDDCNKPLCLNNCYNRG RCVENECVCDEGFTGEDCSELICPNDCFDRGRCINGTCYCEEGFTGEDCGKPTCPHACH TQGRCEEGQCVCDEGFAGVDCSEKRCPADCHNRGRCVDGRCECDDGFTGADCGELKC PNGCSGHGRCVNGQCVCDEGYTGEDCSQLRCPNDCHSRGRCVEGKCVCEQGFKGYDC SDMSCPNDCHQHGRCVNGMCVCDDGYTGEDCRDRQCPRDCSNRGLCVDGQCVCEDG FTGPDCAELSCPNDCHGRGRCVNGQCVCHEGFMGKDCKEQRCPSDCHGQGRCVDGQ CICHEGFTGLDCGQHSCPSDCNNLGQCVSGRCICNEGYSGEDCSEVSPPKDLVVTEVTE ETVNLAWDNEMRVTEYLVVYTPTHEGGLEMQFRVPGDQTSTIIQELEPGVEYFIRVFAI LENKKSIPVSARVATYLPAPEGLKFKSIKETSVEVEWDPLDIAFETWEIIFRNMNKEDEG EITKSLRRPETSYRQTGLAPGQEYEISLHIVKNNTRGPGLKRVTTTRLDAPSQIEVKDVT DTTALITWFKPLAEIDGIELTYGIKDVPGDRTTIDLTEDENQYSIGNLKPDTEYEVSLISRR GDMSSNPAKETFTTGLDAPRNLRRVSQTDNSITLEWRNGKAAIDSYRIKYAPISGGDHA EVDVPKSQQATTKTTLTGLRPGTEYGIGVSAVKEDKESNPATINAATELDTPKDLQVSE TAETSLTLLWKTPLAKFDRYRLNYSLPTGQWVGVQLPRNTTSYVLRGLEPGQEYNVLL TAEKGRHKSKPARVKASTEQAPELENLTVTEVGWDGLRLNWTAADQAYEHFIIQVQE ANKVEAARNLTVPGSLRAVDIPGLKAATPYTVSIYGVIQGYRTPVLSAEASTGETPNLG EVVVAEVGWDALKLNWTAPEGAYEYFFIQVQEADTVEAAQNLTVPGGLRSTDLPGLK AATHYTITIRGVTQDFSTTPLSVEVLTEEVPDMGNLTVTEVSWDALRLNWTTPDGTYD QFTIQVQEADQVEEAHNLTVPGSLRSMEIPGLRAGTPYTVTLHGEVRGHSTRPLAVEVV TEDLPQLGDLAVSEVGWDGLRLNWTAADNAYEHFVIQVQEVNKVEAAQNLTLPGSLR AVDIPGLEAATPYRVSIYGVIRGYRTPVLSAEASTAKEPEIGNLNVSDITPESFNLSWMA TDGIFETFTIEIIDSNRLLETVEYNISGAERTAHISGLPPSTDFIVYLSGLAPSIRTKTISATA T corresponding to amino acids 1-1525 of TENA_HUMAN_V1, which also corresponds to amino acids 1-1525 of HUMTEN_PEA_(—)1_P5 (SEQ ID NO: 934), a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence TEPKPQLGTLIFSNITPKSFNMSWTTQAGLFAKIVINVSDAHSLHESQQFTVSGDAKQAH ITGLVENTGYDVSVAGTTLAGDPTRPLTAFVI (SEQ ID NO: 1144) corresponding to amino acids 1526-1617 of HUMTEN_PEA_(—)1_P5 (SEQ ID NO: 934), and a third amino acid sequence being at least 90% homologous to TEALPLLENLTISDINPYGFTVSWMASENAFDSFLVTVVDSGKLLDPQEFTLSGTQRKLE LRGLITGIGYEVMVSGFTQGHQTKPLRAEIVTEAEPEVDNLLVSDATPDGFRLSWTADE GVFDNFVLKIRDTKKQSEPLEITLLAPERTRDLTGLREATEYEIELYGISKGRRSQTVSAI ATTAMGSPKEVIFSDITENSATVSWRAPTAQVESFRITYVPITGGTPSMVTVDGTKTQTR LVKLIPGVEYLVSIIAMKGFEESEPVSGSFTTALDGPSGLVTANITDSEALARWQPAIATV DSYVISYTGEKVPEITRTVSGNTVEYALTDLEPATEYTLRIFAEKGPQKSSTITAKFTTDL DSPRDLTATEVQSETALLTWRPPRASVTGYLLVYESVDGTVKEVIVGPDTTSYSLADLS PSTHYTAKIQALNGPLRSNMIQTIFTTIGLLYPFPKDCSQAMLNGDTTSGLYTIYLNGDK AQALEVFCDMTSDGGGWIVFLRRKNGRENFYQNWKAYAAGFGDRREEFWLGLDNLN KITAQGQYELRVDLRDHGETAFAVYDKFSVGDAKTRYKLKVEGYSGTAGDSMAYHN GRSFSTFDKDTDSAITNCALSYKGAFWYRNCHRVNLMGRYGDNNHSQGVNWFHWKG HEHSIQFAEMKLRPSNFRNLEGRRKRA corresponding to amino acids 1526-2201 of TENA_HUMAN_V1, which also corresponds to amino acids 1618-2293 of HUMTEN_PEA_(—)1_P5 (SEQ ID NO: 934), wherein said first amino acid sequence, second amino acid sequence and third amino acid sequence are contiguous and in a sequential order.

2. An isolated polypeptide encoding for an edge portion of HUMTEN_PEA_(—)1_P5 (SEQ ID NO: 934), comprising an amino acid sequence being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence encoding for TEPKPQLGTLIFSNITPKSFNMSWTTQAGLFAKIVINVSDAHSLHESQQFTVSGDAKQAH ITGLVENTGYDVSVAGTTLAGDPTRPLTAFVI (SEQ ID NO: 1144), corresponding to HUMTEN_PEA_(—)1_P5 (SEQ ID NO: 934).

It should be noted that the known protein sequence (TENA_HUMAN; SEQ ID NO:933) has one or more changes than the sequence given at the end of the application and named as being the amino acid sequence for TENA_HUMAN_V1 (SEQ ID NO:934). These changes were previously known to occur and are listed in the table below.

TABLE 7 Changes to TENA_HUMAN_V1 SNP position(s) on amino acid sequence Type of change 371 conflict 540 conflict

The location of the variant protein was determined according to results from a number of different software programs and analyses, including analyses from SignalP and other specialized programs. The variant protein is believed to be located as follows with regard to the cell: secreted. The protein localization is believed to be secreted because of manual inspection of known protein localization and/or gene structure.

Variant protein HUMTEN_PEA_(—)1_P5 (SEQ ID NO: 934) also has the following non-silent SNPs (Single Nucleotide Polymorphisms) as listed in Table 8, (given according to their position(s) on the amino acid sequence, with the alternative amino acid(s) listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein HUMTEN_PEA_(—)1_P5 (SEQ ID NO: 934) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 8 Amino acid mutations SNP position(s) on amino acid Previously sequence Alternative amino acid(s) known SNP? 149 Q -> * No 213 G -> S Yes 370 V -> L Yes 539 R -> Q Yes 605 V -> I Yes 680 Q -> R Yes 842 V -> L No 850 D -> H Yes 851 L -> V Yes 1066 R -> H No 1534 T -> M Yes 1769 L -> I Yes 1873 A -> T Yes 2100 Q -> E Yes 2122 K -> No 2130 Q -> No 2159 Q -> No 2265 K -> No 2291 K -> No 2291 K -> Q No

Variant protein HUMTEN_PEA_(—)1_P5 (SEQ ID NO: 934) is encoded by the following transcript(s): HUMTEN_PEA_(—)1_T4 (SEQ ID NO: 857), for which the sequence(s) is/are given at the end of the application. The coding portion of transcript HUMTEN_PEA_(—)1_T4 (SEQ ID NO: 857) is shown in bold; this coding portion starts at position 348 and ends at position 7226. The transcript also has the following SNPs as listed in Table 9 (given according to their position on the nucleotide sequence, with the alternative nucleic acid listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein HUMTEN_PEA_(—)1_P5 (SEQ ID NO: 934) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 9 Nucleic acid SNPs SNP position on nucleotide Previously sequence Alternative nucleic acid known SNP? 115 T -> G Yes 123 A -> G Yes 315 C -> T Yes 434 C -> T Yes 503 C -> T Yes 542 G -> A Yes 623 A -> G Yes 792 C -> T No 984 G -> A Yes 1043 A -> G Yes 1455 G -> T Yes 1963 G -> A Yes 2156 A -> G Yes 2160 G -> A Yes 2386 A -> G Yes 2396 A -> G Yes 2654 G -> A No 2871 G -> T No 2895 G -> C Yes 2898 C -> G Yes 3005 A -> G No 3512 C -> T Yes 3544 G -> A No 3635 A -> G Yes 4922 G -> A No 4948 C -> T Yes 5652 T -> A Yes 5825 A -> G Yes 5964 G -> A Yes 6296 A -> G Yes 6368 C -> A Yes 6645 C -> G Yes 6712 A -> No 6736 A -> No 6824 G -> No 6872 C -> T Yes 7142 G -> No 7218 A -> No 7218 A -> C No 7233 C -> G Yes 7234 C -> G Yes 7236 G -> No 7344 G -> A Yes 7424 A -> G No 7632 A -> C No 7638 T -> C No 7659 -> T No 7828 -> T No 7839 A -> C No 8183 G -> C Yes 8745 G -> T Yes

Variant protein HUMTEN_PEA_(—)1_P6 (SEQ ID NO: 935) according to the present invention has an amino acid sequence as given at the end of the application; it is encoded by transcript(s) HUMTEN_PEA_(—)1_T5 (SEQ ID NO: 858) HUMTEN_PEA_(—)1_T5 (SEQ ID NO: 858) (SEQ ID NO: 859). An alignment is given to the known protein (Tenascin precursor) at the end of the application. One or more alignments to one or more previously published protein sequences are given at the end of the application. A brief description of the relationship of the variant protein according to the present invention to each such aligned protein is as follows:

Comparison report between HUMTEN_PEA_(—)1_P6 (SEQ ID NO: 935) and TENA_HUMAN_V1:

1. An isolated chimeric polypeptide encoding for HUMTEN_PEA_(—)1_P6 (SEQ ID NO: 935), comprising a first amino acid sequence being at least 90% homologous to MGAMTQLLAGVFLAFLALATEGGVLKKVIRHKRQSGVNATLPEENQPVVFNHVYNIK LPVGSQCSVDLESASGEKDLAPPSEPSESFQEHTVDGENQIVFTHRINIPRRACGCAAAP DVKELLSRLEELENLVSSLREQCTAGAGCCLQPATGRLDTRPFCSGRGNFSTEGCGCVC EPGWKGPNCSEPECPGNCHLRGRCIDGQCICDDGFTGEDCSQLACPSDCNDQGKCVNG VCICFEGYAGADCSREICPVPCSEEHGTCVDGLCVCHDGFAGDDCNKPLCLNNCYNRG RCVENECVCDEGFTGEDCSELICPNDCFDRGRCINGTCYCEEGFTGEDCGKPTCPHACH TQGRCEEGQCVCDEGFAGVDCSEKRCPADCHNRGRCVDGRCECDDGFTGADCGELKC PNGCSGHGRCVNGQCVCDEGYTGEDCSQLRCPNDCHSRGRCVEGKCVCEQGFKGYDC SDMSCPNDCHQHGRCVNGMCVCDDGYTGEDCRDRQCPRDCSNRGLCVDGQCVCEDG FTGPDCAELSCPNDCHGRGRCVNGQCVCHEGFMGKDCKEQRCPSDCHGQGRCVDGQ CICHEGFTGLDCGQHSCPSDCNNLGQCVSGRCICNEGYSGEDCSEVSPPKDLVVTEVTE ETVNLAWDNEMRVTEYLVVYTPTHEGGLEMQFRVPGDQTSTIIQELEPGVEYFIRVFAI LENKKSIPVSARVATYLPAPEGLKFKSIKETSVEVEWDPLDIAFETWEIIFRNMNKEDEG EITKSLRRPETSYRQTGLAPGQEYEISLHIVKNNTRGPGLKRVTTTRLDAPSQIEVKDVT DTTALITWFKPLAEIDGIELTYGIKDVPGDRTTIDLTEDENQYSIGNLKPDTEYEVSLISRR GDMSSNPAKETFTTGLDAPRNLRRVSQTDNSITLEWRNGKAAIDSYRIKYAPISGGDHA EVDVPKSQQATTKTTLTGLRPGTEYGIGVSAVKEDKESNPATINAATELDTPKDLQVSE TAETSLTLLWKTPLAKFDRYRLNYSLPTGQWVGVQLPRNTTSYVLRGLEPGQEYNVLL TAEKGRHKSKPARVKASTEQAPELENLTVTEVGWDGLRLNWTAADQAYEHFIIQVQE ANKVEAARNLTVPGSLRAVDIPGLKAATPYTVSIYGVIQGYRTPVLSAEASTGETPNLG EVVVAEVGWDALKLNWTAPEGAYEYFFIQVQEADTVEAAQNLTVPGGLRSTDLPGLK AATHYTITIRGVTQDFSTTPLSVEVLTEEVPDMGNLTVTEVSWDALRLNWTTPDGTYD QFTIQVQEADQVEEAHNLTVPGSLRSMEIPGLRAGTPYTVTLHGEVRGHSTRPLAVEVV TEDLPQLGDLAVSEVGWDGLRLNWTAADNAYEHFVIQVQEVNKVEAAQNLTLPGSLR AVDIPGLEAATPYRVSIYGVIRGYRTPVLSAEASTAKEPEIGNLNVSDITPESFNLSWMA TDGIFETFTIEIIDSNRLLETVEYNISGAERTAHISGLPPSTDFIVYLSGLAPSIRTKTISATA TTE corresponding to amino acids 1-1527 of TENA_HUMAN_V1, which also corresponds to amino acids 1-1527 of HUMTEN_PEA_(—)1_P6 (SEQ ID NO: 935), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence PKPQLGTLIFSNITPKSFNMSWTTQAGLFAKIVINVSDAHSLHESQQFTVSGDAKQAHIT GLVENTGYDVSVAGTTLAGDPTRPLTAFVITGTQSEVLTCLTQREKEISHLKGKFNKNTI FTANVYSLIFN (SEQ ID NO: 1098) corresponding to amino acids 1528-1658 of HUMTEN_PEA_(—)1_P6 (SEQ ID NO: 935), wherein said first amino acid sequence and second amino acid sequence are contiguous and in a sequential order.

2. An isolated polypeptide encoding for a tail of HUMTEN_PEA_(—)1_P6 (SEQ ID NO: 935), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence PKPQLGTLIFSNITPKSFNMSWTTQAGLFAKIVINVSDAHSLHESQQFTVSGDAKQAHIT GLVENTGYDVSVAGTTLAGDPTRPLTAFVITGTQSEVLTCLTQREKEISHLKGKFNKNTI FTANVYSLIFN (SEQ ID NO: 1098) in HUMTEN_PEA_(—)1_P6 (SEQ ID NO: 935).

It should be noted that the known protein sequence (TENA_HUMAN) has one or more changes than the sequence given at the end of the application and named as being the amino acid sequence for TENA_HUMAN_V1. These changes were previously known to occur and are listed in the table below.

TABLE 10 Changes to TENA_HUMAN_V1 SNP position(s) on amino acid sequence Type of change 371 conflict 540 conflict

The location of the variant protein was determined according to results from a number of different software programs and analyses, including analyses from SignalP and other specialized programs. The variant protein is believed to be located as follows with regard to the cell: secreted. The protein localization is believed to be secreted because of manual inspection of known protein localization and/or gene structure.

Variant protein HUMTEN_PEA_(—)1_P6 (SEQ ID NO: 935) also has the following non-silent SNPs (Single Nucleotide Polymorphisms) as listed in Table 11, (given according to their position(s) on the amino acid sequence, with the alternative amino acid(s) listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein HUMTEN_PEA_(—)1_P6 (SEQ ID NO: 935) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 11 Amino acid mutations SNP position(s) on amino acid Previously sequence Alternative amino acid(s) known SNP? 149 Q -> * No 213 G -> S Yes 370 V -> L Yes 539 R -> Q Yes 605 V -> I Yes 680 Q -> R Yes 842 V -> L No 850 D -> H Yes 851 L -> V Yes 1066 R -> H No 1534 T -> M Yes

Variant protein HUMTEN_PEA_(—)1_P6 (SEQ ID NO: 935) is encoded by the following transcript(s): HUMTEN_PEA_(—)1_T5 (SEQ ID NO: 858) HUMTEN_PEA_(—)1_T5 (SEQ ID NO: 858) (SEQ ID NO: 859), for which the sequence(s) is/are given at the end of the application. The coding portion of transcript HUMTEN_PEA_(—)1_T5 (SEQ ID NO: 858) HUMTEN_PEA_(—)1_T5 (SEQ ID NO: 858) (SEQ ID NO: 859) is shown in bold; this coding portion starts at position 348 and ends at position 5321. The transcript also has the following SNPs as listed in Table 12 (given according to their position on the nucleotide sequence, with the alternative nucleic acid listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein HUMTEN_PEA_(—)1_P6 (SEQ ID NO: 935) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 12 Nucleic acid SNPs SNP position on nucleotide Previously sequence Alternative nucleic acid known SNP? 115 T -> G Yes 123 A -> G Yes 315 C -> T Yes 434 C -> T Yes 503 C -> T Yes 542 G -> A Yes 623 A -> G Yes 792 C -> T No 984 G -> A Yes 1043 A -> G Yes 1455 G -> T Yes 1963 G -> A Yes 2156 A -> G Yes 2160 G -> A Yes 2386 A -> G Yes 2396 A -> G Yes 2654 G -> A No 2871 G -> T No 2895 G -> C Yes 2898 C -> G Yes 3005 A -> G No 3512 C -> T Yes 3544 G -> A No 3635 A -> G Yes 4922 G -> A No 4948 C -> T Yes 5210 A -> G Yes 6231 G -> A Yes 6247 A -> C Yes 6354 T -> C Yes 6391 A -> Yes 6394 A -> Yes 6630 C -> A Yes 6766 G -> A Yes 6857 C -> T Yes 6886 C -> T Yes 7330 G -> T Yes 7430 T -> C Yes 7444 A -> G Yes 7690 A -> G No 7821 C -> T Yes 7876 T -> C Yes 8109 T -> A Yes 8424 G -> A Yes 8776 T -> C No 8861 A -> No 8906 G -> C Yes 9016 A -> G Yes 9859 T -> A Yes 10032 A -> G Yes 10171 G -> A Yes 10503 A -> G Yes 10575 C -> A Yes 10852 C -> G Yes 10919 A -> No 10943 A -> No 11031 G -> No 11079 C -> T Yes 11349 G -> No 11425 A -> No 11425 A -> C No 11440 C -> G Yes 11441 C -> G Yes 11443 G -> No 11551 G -> A Yes 11631 A -> G No 11839 A -> C No 11845 T -> C No 11866 -> T No 12035 -> T No 12046 A -> C No 12390 G -> C Yes 12952 G -> T Yes

Variant protein HUMTEN_PEA_(—)1_P7 (SEQ ID NO: 936) according to the present invention has an amino acid sequence as given at the end of the application; it is encoded by transcript(s) HUMTEN_PEA_(—)1_T6 (SEQ ID NO: 859). An alignment is given to the known protein (Tenascin precursor) at the end of the application. One or more alignments to one or more previously published protein sequences are given at the end of the application. A brief description of the relationship of the variant protein according to the present invention to each such aligned protein is as follows:

Comparison report between HUMTEN_PEA_(—)1_P7 (SEQ ID NO: 936) and TENA_HUMAN_V1:

1. An isolated chimeric polypeptide encoding for HUMTEN_PEA_(—)1_P7 (SEQ ID NO: 936), comprising a first amino acid sequence being at least 90% homologous to MGAMTQLLAGVFLAFLALATEGGVLKKVIRHKRQSGVNATLPEENQPVVFNHVYNIK LPVGSQCSVDLESASGEKDLAPPSEPSESFQEHTVDGENQIVFTHRINIPRRACGCAAAP DVKELLSRLEELENLVSSLREQCTAGAGCCLQPATGRLDTRPFCSGRGNFSTEGCGCVC EPGWKGPNCSEPECPGNCHLRGRCIDGQCICDDGFTGEDCSQLACPSDCNDQGKCVNG VCICFEGYAGADCSREICPVPCSEEHGTCVDGLCVCHDGFAGDDCNKPLCLNNCYNRG RCVENECVCDEGFTGEDCSELICPNDCFDRGRCINGTCYCEEGFTGEDCGKPTCPHACH TQGRCEEGQCVCDEGFAGVDCSEKRCPADCHNRGRCVDGRCECDDGFTGADCGELKC PNGCSGHGRCVNGQCVCDEGYTGEDCSQLRCPNDCHSRGRCVEGKCVCEQGFKGYDC SDMSCPNDCHQHGRCVNGMCVCDDGYTGEDCRDRQCPRDCSNRGLCVDGQCVCEDG FTGPDCAELSCPNDCHGRGRCVNGQCVCHEGFMGKDCKEQRCPSDCHGQGRCVDGQ CICHEGFTGLDCGQHSCPSDCNNLGQCVSGRCICNEGYSGEDCSEVSPPKDLVVTEVTE ETVNLAWDNEMRVTEYLVVYTPTHEGGLEMQFRVPGDQTSTIIQELEPGVEYFIRVFAI LENKKSIPVSARVATYLPAPEGLKFKSIKETSVEVEWDPLDIAFETWEIIFRNMNKEDEG EITKSLRRPETSYRQTGLAPGQEYEISLHIVKNNTRGPGLKRVTTTRLDAPSQIEVKDVT DTTALITWFKPLAEIDGIELTYGIKDVPGDRTTIDLTEDENQYSIGNLKPDTEYEVSLISRR GDMSSNPAKETFTTGLDAPRNLRRVSQTDNSITLEWRNGKAAIDSYRIKYAPISGGDHA EVDVPKSQQATTKTTLTGLRPGTEYGIGVSAVKEDKESNPATINAATELDTPKDLQVSE TAETSLTLLWKTPLAKFDRYRLNYSLPTGQWVGVQLPRNTTSYVLRGLEPGQEYNVLL TAEKGRHKSKPARVKASTEQAPELENLTVTEVGWDGLRLNWTAADQAYEHFIIQVQE ANKVEAARNLTVPGSLRAVDIPGLKAATPYTVSIYGVIQGYRTPVLSAEASTGETPNLG EVVVAEVGWDALKLNWTAPEGAYEYFFIQVQEADTVEAAQNLTVPGGLRSTDLPGLK AATHYTITIRGVTQDFSTTPLSVEVLTEEVPDMGNLTVTEVSWDALRLNWTTPDGTYD QFTIQVQEADQVEEAHNLTVPGSLRSMEIPGLRAGTPYTVTLHGEVRGHSTRPLAVEVV TEDLPQLGDLAVSEVGWDGLRLNWTAADNAYEHFVIQVQEVNKVEAAQNLTLPGSLR AVDIPGLEAATPYRVSIYGVIRGYRTPVLSAEASTAKEPEIGNLNVSDITPESFNLSWMA TDGIFETFTIEIIDSNRLLETVEYNISGAERTAHISGLPPSTDFIVYLSGLAPSIRTKTISATA TTEALPLLENLTISDINPYGFTVSWMASENAFDSFLVTVVDSGKLLDPQEFTLSGTQRKL ELRGLITGIGYEVMVSGFTQGHQTKPLRAEIVT corresponding to amino acids 1-1617 of TENA_HUMAN_V1, which also corresponds to amino acids 1-1617 of HUMTEN_PEA_(—)1_P7 (SEQ ID NO: 936), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence GISNQVSHLFLFLVPFCVICLPDRHDFNIFVHIPYLIHKCSLLFHLLPTLPLVICT (SEQ ID NO: 1099) corresponding to amino acids 1618-1673 of HUMTEN_PEA_(—)1_P7 (SEQ ID NO: 936), wherein said first amino acid sequence and second amino acid sequence are contiguous and in a sequential order.

2. An isolated polypeptide encoding for a tail of HUMTEN_PEA_(—)1_P7 (SEQ ID NO: 936), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence GISNQVSHLFLFLVPFCVICLPDRHDFNIFVHIPYLIHKCSLLFHLLPTLPLVICT (SEQ ID NO: 1099) in HUMTEN_PEA_(—)1_P7 (SEQ ID NO: 936).

It should be noted that the known protein sequence (TENA_HUMAN) has one or more changes than the sequence given at the end of the application and named as being the amino acid sequence for TENA_HUMAN_V1. These changes were previously known to occur and are listed in the table below.

TABLE 13 Changes to TENA_HUMAN_V1 SNP position(s) on amino acid sequence Type of change 371 conflict 540 conflict

The location of the variant protein was determined according to results from a number of different software programs and analyses, including analyses from SignalP and other specialized programs. The variant protein is believed to be located as follows with regard to the cell: secreted. The protein localization is believed to be secreted because both signal-peptide prediction programs predict that this protein has a signal peptide, and neither trans-membrane region prediction program predicts that this protein has a trans-membrane region.

Variant protein HUMTEN_PEA_(—)1_P7 (SEQ ID NO: 936) also has the following non-silent SNPs (Single Nucleotide Polymorphisms) as listed in Table 14, (given according to their position(s) on the amino acid sequence, with the alternative amino acid(s) listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein HUMTEN_PEA_(—)1_P7 (SEQ ID NO: 936) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 14 Amino acid mutations SNP position(s) on amino acid Previously sequence Alternative amino acid(s) known SNP? 149 Q -> * No 213 G -> S Yes 370 V -> L Yes 539 R -> Q Yes 605 V -> I Yes 680 Q -> R Yes 842 V -> L No 850 D -> H Yes 851 L -> V Yes 1066 R -> H No

Variant protein HUMTEN_PEA_(—)1_P7 (SEQ ID NO: 936) is encoded by the following transcript(s): HUMTEN_PEA_(—)1_T6 (SEQ ID NO: 859), for which the sequence(s) is/are given at the end of the application. The coding portion of transcript HUMTEN_PEA_(—)1_T6 (SEQ ID NO: 859) is shown in bold; this coding portion starts at position 348 and ends at position 5366. The transcript also has the following SNPs as listed in Table 15 (given according to their position on the nucleotide sequence, with the alternative nucleic acid listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein HUMTEN_PEA_(—)1_P7 (SEQ ID NO: 936) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 15 Nucleic acid SNPs SNP position on nucleotide sequence Alternative nucleic acid Previously known SNP? 115 T -> G Yes 123 A -> G Yes 315 C -> T Yes 434 C -> T Yes 503 C -> T Yes 542 G -> A Yes 623 A -> G Yes 792 C -> T No 984 G -> A Yes 1043 A -> G Yes 1455 G -> T Yes 1963 G -> A Yes 2156 A -> G Yes 2160 G -> A Yes 2386 A -> G Yes 2396 A -> G Yes 2654 G -> A No 2871 G -> T No 2895 G -> C Yes 2898 C -> G Yes 3005 A -> G No 3512 C -> T Yes 3544 G -> A No 3635 A -> G Yes 4922 G -> A No 5488 C -> A Yes 5497 T -> A No 5580 C -> T Yes 5681 A -> G No 5702 G -> A Yes 6202 A -> Yes 6953 T -> A Yes 7126 A -> G Yes 7265 G -> A Yes 7597 A -> G Yes 7669 C -> A Yes 7946 C -> G Yes 8013 A -> No 8037 A -> No 8125 G -> No 8173 C -> T Yes 8443 G -> No 8519 A -> No 8519 A -> C No 8534 C -> G Yes 8535 C -> G Yes 8537 G -> No 8645 G -> A Yes 8725 A -> G No 8933 A -> C No 8939 T -> C No 8960 -> T No 9129 -> T No 9140 A -> C No 9484 G -> C Yes 10046 G -> T Yes

Variant protein HUMTEN_PEA_(—)1_P8 (SEQ ID NO: 937) according to the present invention has an amino acid sequence as given at the end of the application; it is encoded by transcript(s) HUMTEN_PEA_(—)1_T7 (SEQ ID NO: 860). An alignment is given to the known protein (Tenascin precursor) at the end of the application. One or more alignments to one or more previously published protein sequences are given at the end of the application. A brief description of the relationship of the variant protein according to the present invention to each such aligned protein is as follows:

Comparison report between HUMTEN_PEA_(—)1_P8 (SEQ ID NO: 937) and TENA_HUMAN_V1:

1. An isolated chimeric polypeptide encoding for HUMTEN_PEA_(—)1_P8 (SEQ ID NO: 937), comprising a first amino acid sequence being at least 90% homologous to MGAMTQLLAGVFLAFLALATEGGVLKKVIRHKRQSGVNATLPEENQPVVFNHVYNIK LPVGSQCSVDLESASGEKDLAPPSEPSESFQEHTVDGENQIVFTHRINIPRRACGCAAAP DVKELLSRLEELENLVSSLREQCTAGAGCCLQPATGRLDTRPFCSGRGNFSTEGCGCVC EPGWKGPNCSEPECPGNCHLRGRCIDGQCICDDGFTGEDCSQLACPSDCNDQGKCVNG VCICFEGYAGADCSREICPVPCSEEHGTCVDGLCVCHDGFAGDDCNKPLCLNNCYNRG RCVENECVCDEGFTGEDCSELICPNDCFDRGRCINGTCYCEEGFTGEDCGKPTCPHACH TQGRCEEGQCVCDEGFAGVDCSEKRCPADCHNRGRCVDGRCECDDGFTGADCGELKC PNGCSGHGRCVNGQCVCDEGYTGEDCSQLRCPNDCHSRGRCVEGKCVCEQGFKGYDC SDMSCPNDCHQHGRCVNGMCVCDDGYTGEDCRDRQCPRDCSNRGLCVDGQCVCEDG FTGPDCAELSCPNDCHGRGRCVNGQCVCHEGFMGKDCKEQRCPSDCHGQGRCVDGQ CICHEGFTGLDCGQHSCPSDCNNLGQCVSGRCICNEGYSGEDCSEVSPPKDLVVTEVTE ETVNLAWDNEMRVTEYLVVYTPTHEGGLEMQFRVPGDQTSTIIQELEPGVEYFIRVFAI LENKKSIPVSARVATYLPAPEGLKFKSIKETSVEVEWDPLDIAFETWEIIFRNMNKEDEG EITKSLRRPETSYRQTGLAPGQEYEISLHIVKNNTRGPGLKRVTTTRLDAPSQIEVKDVT DTTALITWFKPLAEIDGIELTYGIKDVPGDRTTIDLTEDENQYSIGNLKPDTEYEVSLISRR GDMSSNPAKETFTTGLDAPRNLRRVSQTDNSITLEWRNGKAAIDSYRIKYAPISGGDHA EVDVPKSQQATTKTTLTGLRPGTEYGIGVSAVKEDKESNPATINAATELDTPKDLQVSE TAETSLTLLWKTPLAKFDRYRLNYSLPTGQWVGVQLPRNTTSYVLRGLEPGQEYNVLL TAEKGRHKSKPARVKASTEQAPELENLTVTEVGWDGLRLNWTAADQAYEHFIIQVQE ANKVEAARNLTVPGSLRAVDIPGLKAATPYTVSIYGVIQGYRTPVLSAEASTGETPNLG EVVVAEVGWDALKLNWTAPEGAYEYFFIQVQEADTVEAAQNLTVPGGLRSTDLPGLK AATHYTITIRGVTQDFSTTPLSVEVLTEEVPDMGNLTVTEVSWDALRLNWTTPDGTYD QFTIQVQEADQVEEAHNLTVPGSLRSMEIPGLRAGTPYTVTLHGEVRGHSTRPLAVEVV TEDLPQLGDLAVSEVGWDGLRLNWTAADNAYEHFVIQVQEVNKVEAAQNLTLPGSLR AVDIPGLEAATPYRVSIYGVIRGYRTPVLSAEASTAKEPEIGNLNVSDITPESFNLSWMA TDGIFETFTIEIIDSNRLLETVEYNISGAERTAHISGLPPSTDFIVYLSGLAPSIRTKTISATA T corresponding to amino acids 1-1525 of TENA_HUMAN_V1, which also corresponds to amino acids 1-1525 of HUMTEN_PEA_(—)1_P8 (SEQ ID NO: 937), and a second amino acid sequence being at least 90% homologous to TEAEPEVDNLLVSDATPDGFRLSWTADEGVFDNFVLKIRDTKKQSEPLEITLLAPERTRD LTGLREATEYEIELYGISKGRRSQTVSAIATTAMGSPKEVIFSDITENSATVSWRAPTAQV ESFRITYVPITGGTPSMVTVDGTKTQTRLVKLIPGVEYLVSIIAMKGFEESEPVSGSFTTA LDGPSGLVTANITDSEALARWQPAIATVDSYVISYTGEKVPEITRTVSGNTVEYALTDLE PATEYTLRIFAEKGPQKSSTITAKFTTDLDSPRDLTATEVQSETALLTWRPPRASVTGYL LVYESVDGTVKEVIVGPDTTSYSLADLSPSTHYTAKIQALNGPLRSNMIQTIFTTIGLLYP FPKDCSQAMLNGDTTSGLYTIYLNGDKAQALEVFCDMTSDGGGWIVFLRRKNGRENF YQNWKAYAAGFGDRREEFWLGLDNLNKITAQGQYELRVDLRDHGETAFAVYDKFSV GDAKTRYKLKVEGYSGTAGDSMAYHNGRSFSTFDKDTDSAITNCALSYKGAFWYRNC HRVNLMGRYGDNNHSQGVNWFHWKGHEHSIQFAEMKLRPSNFRNLEGRRKRA corresponding to amino acids 1617-2201 of TENA_HUMAN_V1, which also corresponds to amino acids 1526-2110 of HUMTEN_PEA_(—)1_P8 (SEQ ID NO: 937), wherein said first amino acid sequence and second amino acid sequence are contiguous and in a sequential order.

2. An isolated chimeric polypeptide encoding for an edge portion of HUMTEN_PEA_(—)1_P8 (SEQ ID NO: 937), comprising a polypeptide having a length “n”, wherein n is at least about 10 amino acids in length, optionally at least about 20 amino acids in length, preferably at least about 30 amino acids in length, more preferably at least about 40 amino acids in length and most preferably at least about 50 amino acids in length, wherein at least two amino acids comprise TT, having a structure as follows: a sequence starting from any of amino acid numbers 1525-x to 1525; and ending at any of amino acid numbers 1526+((n−2)-x), in which x varies from 0 to n−2.

It should be noted that the known protein sequence (TENA_HUMAN) has one or more changes than the sequence given at the end of the application and named as being the amino acid sequence for TENA_HUMAN_V1. These changes were previously known to occur and are listed in the table below.

TABLE 16 Changes to TENA_HUMAN_V1 SNP position(s) on amino acid sequence Type of change 371 conflict 540 conflict

The location of the variant protein was determined according to results from a number of different software programs and analyses, including analyses from SignalP and other specialized programs. The variant protein is believed to be located as follows with regard to the cell: secreted. The protein localization is believed to be secreted because of manual inspection of known protein localization and/or gene structure.

Variant protein HUMTEN_PEA_(—)1_P8 (SEQ ID NO: 937) also has the following non-silent SNPs (Single Nucleotide Polymorphisms) as listed in Table 17, (given according to their position(s) on the amino acid sequence, with the alternative amino acid(s) listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein HUMTEN_PEA_(—)1_P8 (SEQ ID NO: 937) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 17 Amino acid mutations SNP position(s) on amino acid sequence Alternative amino acid(s) Previously known SNP? 149 Q -> * No 213 G -> S Yes 370 V -> L Yes 539 R -> Q Yes 605 V -> I Yes 680 Q -> R Yes 842 V -> L No 850 D -> H Yes 851 L -> V Yes 1066 R -> H No 1586 L -> I Yes 1690 A -> T Yes 1917 Q -> E Yes 1939 K -> No 1947 Q -> No 1976 Q -> No 2082 K -> No 2108 K -> No 2108 K -> Q No

Variant protein HUMTEN_PEA_(—)1_P8 (SEQ ID NO: 937) is encoded by the following transcript(s): HUMTEN_PEA_(—)1_T7 (SEQ ID NO: 860), for which the sequence(s) is/are given at the end of the application. The coding portion of transcript HUMTEN_PEA_(—)1_T7 (SEQ ID NO: 860) is shown in bold; this coding portion starts at position 348 and ends at position 6677. The transcript also has the following SNPs as listed in Table 18 (given according to their position on the nucleotide sequence, with the alternative nucleic acid listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein HUMTEN_PEA_(—)1_P8 (SEQ ID NO: 937) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 18 Nucleic acid SNPs SNP position on nucleotide sequence Alternative nucleic acid Previously known SNP? 115 T -> G Yes 123 A -> G Yes 315 C -> T Yes 434 C -> T Yes 503 C -> T Yes 542 G -> A Yes 623 A -> G Yes 792 C -> T No 984 G -> A Yes 1043 A -> G Yes 1455 G -> T Yes 1963 G -> A Yes 2156 A -> G Yes 2160 G -> A Yes 2386 A -> G Yes 2396 A -> G Yes 2654 G -> A No 2871 G -> T No 2895 G -> C Yes 2898 C -> G Yes 3005 A -> G No 3512 C -> T Yes 3544 G -> A No 3635 A -> G Yes 4922 G -> A No 5103 T -> A Yes 5276 A -> G Yes 5415 G -> A Yes 5747 A -> G Yes 5819 C -> A Yes 6096 C -> G Yes 6163 A -> No 6187 A -> No 6275 G -> No 6323 C -> T Yes 6593 G -> No 6669 A -> No 6669 A -> C No 6684 C -> G Yes 6685 C -> G Yes 6687 G -> No 6795 G -> A Yes 6875 A -> G No 7083 A -> C No 7089 T -> C No 7110 -> T No 7279 -> T No 7290 A -> C No 7634 G -> C Yes 8196 G -> T Yes

Variant protein HUMTEN_PEA_(—)1_P10 (SEQ ID NO: 938) according to the present invention has an amino acid sequence as given at the end of the application; it is encoded by transcript(s) HUMTEN_PEA_(—)1_T11 (SEQ ID NO: 861). An alignment is given to the known protein (Tenascin precursor) at the end of the application. One or more alignments to one or more previously published protein sequences are given at the end of the application. A brief description of the relationship of the variant protein according to the present invention to each such aligned protein is as follows:

Comparison report between HUMTEN_PEA_(—)1_P10 (SEQ ID NO: 938) and TENA_HUMAN_V1:

1. An isolated chimeric polypeptide encoding for HUMTEN_PEA_(—)1_P10 (SEQ ID NO: 938), comprising a first amino acid sequence being at least 90% homologous to MGAMTQLLAGVFLAFLALATEGGVLKKVIRHKRQSGVNATLPEENQPVVFNHVYNIK LPVGSQCSVDLESASGEKDLAPPSEPSESFQEHTVDGENQIVFTHRINIPRRACGCAAAP DVKELLSRLEELENLVSSLREQCTAGAGCCLQPATGRLDTRPFCSGRGNFSTEGCGCVC EPGWKGPNCSEPECPGNCHLRGRCIDGQCICDDGFTGEDCSQLACPSDCNDQGKCVNG VCICFEGYAGADCSREICPVPCSEEHGTCVDGLCVCHDGFAGDDCNKPLCLNNCYNRG RCVENECVCDEGFTGEDCSELICPNDCFDRGRCINGTCYCEEGFTGEDCGKPTCPHACH TQGRCEEGQCVCDEGFAGVDCSEKRCPADCHNRGRCVDGRCECDDGFTGADCGELKC PNGCSGHGRCVNGQCVCDEGYTGEDCSQLRCPNDCHSRGRCVEGKCVCEQGFKGYDC SDMSCPNDCHQHGRCVNGMCVCDDGYTGEDCRDRQCPRDCSNRGLCVDGQCVCEDG FTGPDCAELSCPNDCHGRGRCVNGQCVCHEGFMGKDCKEQRCPSDCHGQGRCVDGQ CICHEGFTGLDCGQHSCPSDCNNLGQCVSGRCICNEGYSGEDCSEVSPPKDLVVTEVTE ETVNLAWDNEMRVTEYLVVYTPTHEGGLEMQFRVPGDQTSTIIQELEPGVEYFIRVFAI LENKKSIPVSARVATYLPAPEGLKFKSIKETSVEVEWDPLDIAFETWEIIFRNMNKEDEG EITKSLRRPETSYRQTGLAPGQEYEISLHIVKNNTRGPGLKRVTTTRLDAPSQIEVKDVT DTTALITWFKPLAEIDGIELTYGIKDVPGDRTTIDLTEDENQYSIGNLKPDTEYEVSLISRR GDMSSNPAKETFTTGLDAPRNLRRVSQTDNSITLEWRNGKAAIDSYRIKYAPISGGDHA EVDVPKSQQATTKTTLTGLRPGTEYGIGVSAVKEDKESNPATINAATELDTPKDLQVSE TAETSLTLLWKTPLAKFDRYRLNYSLPTGQWVGVQLPRNTTSYVLRGLEPGQEYNVLL TAEKGRHKSKPARVKASTEQAPELENLTVTEVGWDGLRLNWTAADQAYEHFIIQVQE ANKVEAARNLTVPGSLRAVDIPGLKAATPYTVSIYGVIQGYRTPVLSAEASTGETPNLG EVVVAEVGWDALKLNWTAPEGAYEYFFIQVQEADTVEAAQNLTVPGGLRSTDLPGLK AATHYTITIRGVTQDFSTTPLSVEVL corresponding to amino acids 1-1252 of TENA_HUMAN_V1, which also corresponds to amino acids 1-1252 of HUMTEN_PEA_(—)1_P10 (SEQ ID NO: 938), and a second amino acid sequence being at least 90% homologous to TEDLPQLGDLAVSEVGWDGLRLNWTAADNAYEHFVIQVQEVNKVEAAQNLTLPGSLR AVDIPGLEAATPYRVSIYGVIRGYRTPVLSAEASTAKEPEIGNLNVSDITPESFNLSWMA TDGIFETFTIEIIDSNRLLETVEYNISGAERTAHISGLPPSTDFIVYLSGLAPSIRTKTISATA TTEALPLLENLTISDINPYGFTVSWMASENAFDSFLVTVVDSGKLLDPQEFTLSGTQRKL ELRGLITGIGYEVMVSGFTQGHQTKPLRAEIVTEAEPEVDNLLVSDATPDGFRLSWTAD EGVFDNFVLKIRDTKKQSEPLEITLLAPERTRDLTGLREATEYEIELYGISKGRRSQTVSA IATTAMGSPKEVIFSDITENSATVSWRAPTAQVESFRITYVPITGGTPSMVTVDGTKTQT RLVKLIPGVEYLVSIIAMKGFEESEPVSGSFTTALDGPSGLVTANITDSEALARWQPAIAT VDSYVISYTGEKVPEITRTVSGNTVEYALTDLEPATEYTLRIFAEKGPQKSSTITAKFTTD LDSPRDLTATEVQSETALLTWRPPRASVTGYLLVYESVDGTVKEVIVGPDTTSYSLADL SPSTHYTAKIQALNGPLRSNMIQTIFTTIGLLYPFPKDCSQAMLNGDTTSGLYTIYLNGD KAQALEVFCDMTSDGGGWIVFLRRKNGRENFYQNWKAYAAGFGDRREEFWLGLDNL NKITAQGQYELRVDLRDHGETAFAVYDKFSVGDAKTRYKLKVEGYSGTAGDSMAYH NGRSFSTFDKDTDSAITNCALSYKGAFWYRNCHRVNLMGRYGDNNHSQGVNWFHWK GHEHSIQFAEMKLRPSNFRNLEGRRKRA corresponding to amino acids 1344-2201 of TENA_HUMAN_V1, which also corresponds to amino acids 1253-2110 of HUMTEN_PEA_(—)1_P10 (SEQ ID NO: 938), wherein said first amino acid sequence and second amino acid sequence are contiguous and in a sequential order.

2. An isolated chimeric polypeptide encoding for an edge portion of HUMTEN_PEA_(—)1_P10 (SEQ ID NO: 938), comprising a polypeptide having a length “n”, wherein n is at least about 10 amino acids in length, optionally at least about 20 amino acids in length, preferably at least about 30 amino acids in length, more preferably at least about 40 amino acids in length and most preferably at least about 50 amino acids in length, wherein at least two amino acids comprise LT, having a structure as follows: a sequence starting from any of amino acid numbers 1252-x to 1252; and ending at any of amino acid numbers 1253+((n−2)-x), in which x varies from 0 to n−2.

It should be noted that the known protein sequence (TENA_HUMAN) has one or more changes than the sequence given at the end of the application and named as being the amino acid sequence for TENA_HUMAN_V1. These changes were previously known to occur and are listed in the table below.

TABLE 19 Changes to TENA_HUMAN_V1 SNP position(s) on amino acid sequence Type of change 371 conflict 540 conflict

The location of the variant protein was determined according to results from a number of different software programs and analyses, including analyses from SignalP and other specialized programs. The variant protein is believed to be located as follows with regard to the cell: secreted. The protein localization is believed to be secreted because of manual inspection of known protein localization and/or gene structure.

Variant protein HUMTEN_PEA_(—)1_P10 (SEQ ID NO: 938) also has the following non-silent SNPs (Single Nucleotide Polymorphisms) as listed in Table 20, (given according to their position(s) on the amino acid sequence, with the alternative amino acid(s) listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein HUMTEN_PEA_(—)1_P10 (SEQ ID NO: 938) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 20 Amino acid mutations SNP position(s) on amino acid sequence Alternative amino acid(s) Previously known SNP? 149 Q -> * No 213 G -> S Yes 370 V -> L Yes 539 R -> Q Yes 605 V -> I Yes 680 Q -> R Yes 842 V -> L No 850 D -> H Yes 851 L -> V Yes 1066 R -> H No 1586 L -> I Yes 1690 A -> T Yes 1917 Q -> E Yes 1939 K -> No 1947 Q -> No 1976 Q -> No 2082 K -> No 2108 K -> No 2108 K -> Q No

Variant protein HUMTEN_PEA_(—)1_P10 (SEQ ID NO: 938) is encoded by the following transcript(s): HUMTEN_PEA_(—)1_T11 (SEQ ID NO: 861), for which the sequence(s) is/are given at the end of the application. The coding portion of transcript HUMTEN_PEA_(—)1_T11 (SEQ ID NO: 861) is shown in bold; this coding portion starts at position 348 and ends at position 6677. The transcript also has the following SNPs as listed in Table 21 (given according to their position on the nucleotide sequence, with the alternative nucleic acid listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein HUMTEN_PEA_(—)1_P10 (SEQ ID NO: 938) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 21 Nucleic acid SNPs SNP position on nucleotide sequence Alternative nucleic acid Previously known SNP? 115 T -> G Yes 123 A -> G Yes 315 C -> T Yes 434 C -> T Yes 503 C -> T Yes 542 G -> A Yes 623 A -> G Yes 792 C -> T No 984 G -> A Yes 1043 A -> G Yes 1455 G -> T Yes 1963 G -> A Yes 2156 A -> G Yes 2160 G -> A Yes 2386 A -> G Yes 2396 A -> G Yes 2654 G -> A No 2871 G -> T No 2895 G -> C Yes 2898 C -> G Yes 3005 A -> G No 3512 C -> T Yes 3544 G -> A No 3635 A -> G Yes 4649 G -> A No 5103 T -> A Yes 5276 A -> G Yes 5415 G -> A Yes 5747 A -> G Yes 5819 C -> A Yes 6096 C -> G Yes 6163 A -> No 6187 A -> No 6275 G -> No 6323 C -> T Yes 6593 G -> No 6669 A -> No 6669 A -> C No 6684 C -> G Yes 6685 C -> G Yes 6687 G -> No 6795 G -> A Yes 6875 A -> G No 7083 A -> C No 7089 T -> C No 7110 -> T No 7279 -> T No 7290 A -> C No 7634 G -> C Yes 8196 G -> T Yes

Variant protein HUMTEN_PEA_(—)1_P11 (SEQ ID NO: 939) according to the present invention has an amino acid sequence as given at the end of the application; it is encoded by transcript(s) HUMTEN_PEA_(—)1_T14 (SEQ ID NO: 862). An alignment is given to the known protein (Tenascin precursor) at the end of the application. One or more alignments to one or more previously published protein sequences are given at the end of the application. A brief description of the relationship of the variant protein according to the present invention to each such aligned protein is as follows:

Comparison report between HUMTEN_PEA_(—)1_P11 (SEQ ID NO: 939) and TENA_HUMAN_V1:

1. An isolated chimeric polypeptide encoding for HUMTEN_PEA_(—)1_P11 (SEQ ID NO: 939), comprising a first amino acid sequence being at least 90% homologous to MGAMTQLLAGVFLAFLALATEGGVLKKVIRHKRQSGVNATLPEENQPVVFNHVYNIK LPVGSQCSVDLESASGEKDLAPPSEPSESFQEHTVDGENQIVFTHRINIPRRACGCAAAP DVKELLSRLEELENLVSSLREQCTAGAGCCLQPATGRLDTRPFCSGRGNFSTEGCGCVC EPGWKGPNCSEPECPGNCHLRGRCIDGQCICDDGFTGEDCSQLACPSDCNDQGKCVNG VCICFEGYAGADCSREICPVPCSEEHGTCVDGLCVCHDGFAGDDCNKPLCLNNCYNRG RCVENECVCDEGFTGEDCSELICPNDCFDRGRCINGTCYCEEGFTGEDCGKPTCPHACH TQGRCEEGQCVCDEGFAGVDCSEKRCPADCHNRGRCVDGRCECDDGFTGADCGELKC PNGCSGHGRCVNGQCVCDEGYTGEDCSQLRCPNDCHSRGRCVEGKCVCEQGFKGYDC SDMSCPNDCHQHGRCVNGMCVCDDGYTGEDCRDRQCPRDCSNRGLCVDGQCVCEDG FTGPDCAELSCPNDCHGRGRCVNGQCVCHEGFMGKDCKEQRCPSDCHGQGRCVDGQ CICHEGFTGLDCGQHSCPSDCNNLGQCVSGRCICNEGYSGEDCSEVSPPKDLVVTEVTE ETVNLAWDNEMRVTEYLVVYTPTHEGGLEMQFRVPGDQTSTIIQELEPGVEYFIRVFAI LENKKSIPVSARVATYLPAPEGLKFKSIKETSVEVEWDPLDIAFETWEIIFRNMNKEDEG EITKSLRRPETSYRQTGLAPGQEYEISLHIVKNNTRGPGLKRVTTTRLDAPSQIEVKDVT DTTALITWFKPLAEIDGIELTYGIKDVPGDRTTIDLTEDENQYSIGNLKPDTEYEVSLISRR GDMSSNPAKETFTTGLDAPRNLRRVSQTDNSITLEWRNGKAAIDSYRIKYAPISGGDHA EVDVPKSQQATTKTTLTGLRPGTEYGIGVSAVKEDKESNPATINAATELDTPKDLQVSE TAETSLTLLWKTPLAKFDRYRLNYSLPTGQWVGVQLPRNTTSYVLRGLEPGQEYNVLL TAEKGRHKSKPARVKASTEQAPELENLTVTEVGWDGLRLNWTAADQAYEHFIIQVQE ANKVEAARNLTVPGSLRAVDIPGLKAATPYTVSIYGVIQ corresponding to amino acids 1-1149 of TENA_HUMAN_V1, which also corresponds to amino acids 1-1149 of HUMTEN_PEA_(—)1_P11 (SEQ ID NO: 939), and a second amino acid sequence being at least 90% homologous to GYRTPVLSAEASTAKEPEIGNLNVSDITPESFNLSWMATDGIFETFTIEIIDSNRLLETVEY NISGAERTAHISGLPPSTDFIVYLSGLAPSIRTKTISATATTEALPLLENLTISDINPYGFTV SWMASENAFDSFLVTVVDSGKLLDPQEFTLSGTQRKLELRGLITGIGYEVMVSGFTQGH QTKPLRAEIVTEAEPEVDNLLVSDATPDGFRLSWTADEGVFDNFVLKIRDTKKQSEPLEI TLLAPERTRDLTGLREATEYEIELYGISKGRRSQTVSAIATTAMGSPKEVIFSDITENSAT VSWRAPTAQVESFRITYVPITGGTPSMVTVDGTKTQTRLVKLIPGVEYLVSIIAMKGFEE SEPVSGSFTTALDGPSGLVTANITDSEALARWQPAIATVDSYVISYTGEKVPEITRTVSG NTVEYALTDLEPATEYTLRIFAEKGPQKSSTITAKFTTDLDSPRDLTATEVQSETALLTW RPPRASVTGYLLVYESVDGTVKEVIVGPDTTSYSLADLSPSTHYTAKIQALNGPLRSNMI QTIFTTIGLLYPFPKDCSQAMLNGDTTSGLYTIYLNGDKAQALEVFCDMTSDGGGWIVF LRRKNGRENFYQNWKAYAAGFGDRREEFWLGLDNLNKITAQGQYELRVDLRDHGET AFAVYDKFSVGDAKTRYKLKVEGYSGTAGDSMAYHNGRSFSTFDKDTDSAITNCALS YKGAFWYRNCHRVNLMGRYGDNNHSQGVNWFHWKGHEHSIQFAEMKLRPSNFRNLE GRRKRA corresponding to amino acids 1423-2201 of TENA_HUMAN_V1, which also corresponds to amino acids 1150-1928 of HUMTEN_PEA_(—)1_P11 (SEQ ID NO: 939), wherein said first amino acid sequence and second amino acid sequence are contiguous and in a sequential order.

2. An isolated chimeric polypeptide encoding for an edge portion of HUMTEN_PEA_(—)1_P11 (SEQ ID NO: 939), comprising a polypeptide having a length “n”, wherein n is at least about 10 amino acids in length, optionally at least about 20 amino acids in length, preferably at least about 30 amino acids in length, more preferably at least about 40 amino acids in length and most preferably at least about 50 amino acids in length, wherein at least two amino acids comprise QG, having a structure as follows: a sequence starting from any of amino acid numbers 1149-x to 1149; and ending at any of amino acid numbers 1150+((n−2)-x), in which x varies from 0 to n−2.

It should be noted that the known protein sequence (TENA_HUMAN) has one or more changes than the sequence given at the end of the application and named as being the amino acid sequence for TENA_HUMAN_V1. These changes were previously known to occur and are listed in the table below.

TABLE 22 Changes to TENA_HUMAN_V1 SNP position(s) on amino acid sequence Type of change 371 conflict 540 conflict

The location of the variant protein was determined according to results from a number of different software programs and analyses, including analyses from SignalP and other specialized programs. The variant protein is believed to be located as follows with regard to the cell: secreted. The protein localization is believed to be secreted because of manual inspection of known protein localization and/or gene structure.

Variant protein HUMTEN_PEA_(—)1_P11 (SEQ ID NO: 939) also has the following non-silent SNPs (Single Nucleotide Polymorphisms) as listed in Table 23, (given according to their position(s) on the amino acid sequence, with the alternative amino acid(s) listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein HUMTEN_PEA_(—)1_P11 (SEQ ID NO: 939) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 23 Amino acid mutations SNP position(s) on amino acid sequence Alternative amino acid(s) Previously known SNP? 149 Q -> * No 213 G -> S Yes 370 V -> L Yes 539 R -> Q Yes 605 V -> I Yes 680 Q -> R Yes 842 V -> L No 850 D -> H Yes 851 L -> V Yes 1066 R -> H No 1404 L -> I Yes 1508 A -> T Yes 1735 Q -> E Yes 1757 K -> No 1765 Q -> No 1794 Q -> No 1900 K -> No 1926 K -> No 1926 K -> Q No

Variant protein HUMTEN_PEA_(—)1_P11 (SEQ ID NO: 939) is encoded by the following transcript(s): HUMTEN_PEA_(—)1_T14 (SEQ ID NO: 862), for which the sequence(s) is/are given at the end of the application. The coding portion of transcript HUMTEN_PEA_(—)1_T14 (SEQ ID NO: 862) is shown in bold; this coding portion starts at position 348 and ends at position 6131. The transcript also has the following SNPs as listed in Table 24 (given according to their position on the nucleotide sequence, with the alternative nucleic acid listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein HUMTEN_PEA_(—)1_P11 (SEQ ID NO: 939) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 24 Nucleic acid SNPs SNP position on nucleotide sequence Alternative nucleic acid Previously known SNP? 115 T -> G Yes 123 A -> G Yes 315 C -> T Yes 434 C -> T Yes 503 C -> T Yes 542 G -> A Yes 623 A -> G Yes 792 C -> T No 984 G -> A Yes 1043 A -> G Yes 1455 G -> T Yes 1963 G -> A Yes 2156 A -> G Yes 2160 G -> A Yes 2386 A -> G Yes 2396 A -> G Yes 2654 G -> A No 2871 G -> T No 2895 G -> C Yes 2898 C -> G Yes 3005 A -> G No 3512 C -> T Yes 3544 G -> A No 3635 A -> G Yes 4103 G -> A No 4557 T -> A Yes 4730 A -> G Yes 4869 G -> A Yes 5201 A -> G Yes 5273 C -> A Yes 5550 C -> G Yes 5617 A -> No 5641 A -> No 5729 G -> No 5777 C -> T Yes 6047 G -> No 6123 A -> No 6123 A -> C No 6138 C -> G Yes 6139 C -> G Yes 6141 G -> No 6249 G -> A Yes 6329 A -> G No 6537 A -> C No 6543 T -> C No 6564 -> T No 6733 -> T No 6744 A -> C No 7088 G -> C Yes 7650 G -> T Yes

Variant protein HUMTEN_PEA_(—)1_P13 (SEQ ID NO: 940) according to the present invention has an amino acid sequence as given at the end of the application; it is encoded by transcript(s) HUMTEN_PEA_(—)1_T16 (SEQ ID NO: 863). An alignment is given to the known protein (Tenascin precursor) at the end of the application. One or more alignments to one or more previously published protein sequences are given at the end of the application. A brief description of the relationship of the variant protein according to the present invention to each such aligned protein is as follows:

Comparison report between HUMTEN_PEA_(—)1_P13 (SEQ ID NO: 940) and TENA_HUMAN_V1:

1. An isolated chimeric polypeptide encoding for HUMTEN_PEA_(—)1_P13 (SEQ ID NO: 940), comprising a first amino acid sequence being at least 90% homologous to MGAMTQLLAGVFLAFLALATEGGVLKKVIRHKRQSGVNATLPEENQPVVFNHVYNIK LPVGSQCSVDLESASGEKDLAPPSEPSESFQEHTVDGENQIVFTHRINIPRRACGCAAAP DVKELLSRLEELENLVSSLREQCTAGAGCCLQPATGRLDTRPFCSGRGNFSTEGCGCVC EPGWKGPNCSEPECPGNCHLRGRCIDGQCICDDGFTGEDCSQLACPSDCNDQGKCVNG VCICFEGYAGADCSREICPVPCSEEHGTCVDGLCVCHDGFAGDDCNKPLCLNNCYNRG RCVENECVCDEGFTGEDCSELICPNDCFDRGRCINGTCYCEEGFTGEDCGKPTCPHACH TQGRCEEGQCVCDEGFAGVDCSEKRCPADCHNRGRCVDGRCECDDGFTGADCGELKC PNGCSGHGRCVNGQCVCDEGYTGEDCSQLRCPNDCHSRGRCVEGKCVCEQGFKGYDC SDMSCPNDCHQHGRCVNGMCVCDDGYTGEDCRDRQCPRDCSNRGLCVDGQCVCEDG FTGPDCAELSCPNDCHGRGRCVNGQCVCHEGFMGKDCKEQRCPSDCHGQGRCVDGQ CICHEGFTGLDCGQHSCPSDCNNLGQCVSGRCICNEGYSGEDCSEVSPPKDLVVTEVTE ETVNLAWDNEMRVTEYLVVYTPTHEGGLEMQFRVPGDQTSTIIQELEPGVEYFIRVFAI LENKKSIPVSARVATYLPAPEGLKFKSIKETSVEVEWDPLDIAFETWEIIFRNMNKEDEG EITKSLRRPETSYRQTGLAPGQEYEISLHIVKNNTRGPGLKRVTTTRLDAPSQIEVKDVT DTTALITWFKPLAEIDGIELTYGIKDVPGDRTTIDLTEDENQYSIGNLKPDTEYEVSLISRR GDMSSNPAKETFTTGLDAPRNLRRVSQTDNSITLEWRNGKAAIDSYRIKYAPISGGDHA EVDVPKSQQATTKTTLTGLRPGTEYGIGVSAVKEDKESNPATINAATELDTPKDLQVSE TAETSLTLLWKTPLAKFDRYRLNYSLPTGQWVGVQLPRNTTSYVLRGLEPGQEYNVLL TAEKGRHKSKPARVKASTEQAPELENLTVTEVGWDGLRLNWTAADQAYEHFIIQVQE ANKVEAARNLTVPGSLRAVDIPGLKAATPYTVSIYGVIQGYRTPVLSAEASTGETPNLG EVVVAEVGWDALKLNWTAPEGAYEYFFIQVQEADTVEAAQNLTVPGGLRSTDLPGLK AATHYTITIRGVTQDFSTTPLSVEVLTEEVPDMGNLTVTEVSWDALRLNWTTPDGTYD QFTIQVQEADQVEEAHNLTVPGSLRSMEIPGLRAGTPYTVTLHGEVRGHSTRPLAVEVV corresponding to amino acids 1-1343 of TENA_HUMAN_V1, which also corresponds to amino acids 1-1343 of HUMTEN_PEA_(—)1_P13 (SEQ ID NO: 940), and a second amino acid sequence being at least 90% homologous to TAMGSPKEVIFSDITENSATVSWRAPTAQVESFRITYVPITGGTPSMVTVDGTKTQTRLV KLIPGVEYLVSIIAMKGFEESEPVSGSFTTALDGPSGLVTANITDSEALARWQPAIATVDS YVISYTGEKVPEITRTVSGNTVEYALTDLEPATEYTLRIFAEKGPQKSSTITAKFTTDLDS PRDLTATEVQSETALLTWRPPRASVTGYLLVYESVDGTVKEVIVGPDTTSYSLADLSPS THYTAKIQALNGPLRSNMIQTIFTTIGLLYPFPKDCSQAMLNGDTTSGLYTIYLNGDKAQ ALEVFCDMTSDGGGWIVFLRRKNGRENFYQNWKAYAAGFGDRREEFWLGLDNLNKIT AQGQYELRVDLRDHGETAFAVYDKFSVGDAKTRYKLKVEGYSGTAGDSMAYHNGRS FSTFDKDTDSAITNCALSYKGAFWYRNCHRVNLMGRYGDNNHSQGVNWFHWKGHEH SIQFAEMKLRPSNFRNLEGRRKRA corresponding to amino acids 1708-2201 of TENA_HUMAN_V1, which also corresponds to amino acids 1344-1837 of HUMTEN_PEA_(—)1_P13 (SEQ ID NO: 940), wherein said first amino acid sequence and second amino acid sequence are contiguous and in a sequential order.

2. An isolated chimeric polypeptide encoding for an edge portion of HUMTEN_PEA_(—)1_P13 (SEQ ID NO: 940), comprising a polypeptide having a length “n”, wherein n is at least about 10 amino acids in length, optionally at least about 20 amino acids in length, preferably at least about 30 amino acids in length, more preferably at least about 40 amino acids in length and most preferably at least about 50 amino acids in length, wherein at least two amino acids comprise VT, having a structure as follows: a sequence starting from any of amino acid numbers 1343-x to 1343; and ending at any of amino acid numbers 1344+((n−2)-x), in which x varies from 0 to n−2.

It should be noted that the known protein sequence (TENA_HUMAN) has one or more changes than the sequence given at the end of the application and named as being the amino acid sequence for TENA_HUMAN_V1. These changes were previously known to occur and are listed in the table below.

TABLE 25 Changes to TENA_HUMAN_V1 SNP position(s) on amino acid sequence Type of change 371 conflict 540 conflict

The location of the variant protein was determined according to results from a number of different software programs and analyses, including analyses from SignalP and other specialized programs. The variant protein is believed to be located as follows with regard to the cell: secreted. The protein localization is believed to be secreted because of manual inspection of known protein localization and/or gene structure.

Variant protein HUMTEN_PEA_(—)1_P13 (SEQ ID NO: 940) also has the following non-silent SNPs (Single Nucleotide Polymorphisms) as listed in Table 26, (given according to their position(s) on the amino acid sequence, with the alternative amino acid(s) listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein HUMTEN_PEA_(—)1_P13 (SEQ ID NO: 940) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 26 Amino acid mutations SNP position(s) on amino acid sequence Alternative amino acid(s) Previously known SNP? 149 Q -> * No 213 G -> S Yes 370 V -> L Yes 539 R -> Q Yes 605 V -> I Yes 680 Q -> R Yes 842 V -> L No 850 D -> H Yes 851 L -> V Yes 1066 R -> H No 1417 A -> T Yes 1644 Q -> E Yes 1666 K -> No 1674 Q -> No 1703 Q -> No 1809 K -> No 1835 K -> No 1835 K -> Q No

Variant protein HUMTEN_PEA_(—)1_P13 (SEQ ID NO: 940) is encoded by the following transcript(s): HUMTEN_PEA_(—)1_T16 (SEQ ID NO: 863), for which the sequence(s) is/are given at the end of the application. The coding portion of transcript HUMTEN_PEA_(—)1_T16 (SEQ ID NO: 863) is shown in bold; this coding portion starts at position 348 and ends at position 5858. The transcript also has the following SNPs as listed in Table 27 (given according to their position on the nucleotide sequence, with the alternative nucleic acid listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein HUMTEN_PEA_(—)1_P13 (SEQ ID NO: 940) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 27 Nucleic acid SNPs SNP position on nucleotide sequence Alternative nucleic acid Previously known SNP? 115 T -> G Yes 123 A -> G Yes 315 C -> T Yes 434 C -> T Yes 503 C -> T Yes 542 G -> A Yes 623 A -> G Yes 792 C -> T No 984 G -> A Yes 1043 A -> G Yes 1455 G -> T Yes 1963 G -> A Yes 2156 A -> G Yes 2160 G -> A Yes 2386 A -> G Yes 2396 A -> G Yes 2654 G -> A No 2871 G -> T No 2895 G -> C Yes 2898 C -> G Yes 3005 A -> G No 3512 C -> T Yes 3544 G -> A No 3635 A -> G Yes 4457 A -> G Yes 4596 G -> A Yes 4928 A -> G Yes 5000 C -> A Yes 5277 C -> G Yes 5344 A -> No 5368 A -> No 5456 G -> No 5504 C -> T Yes 5774 G -> No 5850 A -> No 5850 A -> C No 5865 C -> G Yes 5866 C -> G Yes 5868 G -> No 5976 G -> A Yes 6056 A -> G No 6264 A -> C No 6270 T -> C No 6291 -> T No 6460 -> T No 6471 A -> C No 6815 G -> C Yes 7377 G -> T Yes

Variant protein HUMTEN_PEA_(—)1_P14 (SEQ ID NO: 941) according to the present invention has an amino acid sequence as given at the end of the application; it is encoded by transcript(s) HUMTEN_PEA_(—)1_T17 (SEQ ID NO: 864). An alignment is given to the known protein (Tenascin precursor) at the end of the application. One or more alignments to one or more previously published protein sequences are given at the end of the application. A brief description of the relationship of the variant protein according to the present invention to each such aligned protein is as follows:

Comparison report between HUMTEN_PEA_(—)1_P14 (SEQ ID NO: 941) and TENA_HUMAN_V1:

1. An isolated chimeric polypeptide encoding for HUMTEN_PEA_(—)1_P14 (SEQ ID NO: 941), comprising a first amino acid sequence being at least 90% homologous to MGAMTQLLAGVFLAFLALATEGGVLKKVIRHKRQSGVNATLPEENQPVVFNHVYNIK LPVGSQCSVDLESASGEKDLAPPSEPSESFQEHTVDGENQIVFTHRINIPRRACGCAAAP DVKELLSRLEELENLVSSLREQCTAGAGCCLQPATGRLDTRPFCSGRGNFSTEGCGCVC EPGWKGPNCSEPECPGNCHLRGRCIDGQCICDDGFTGEDCSQLACPSDCNDQGKCVNG VCICFEGYAGADCSREICPVPCSEEHGTCVDGLCVCHDGFAGDDCNKPLCLNNCYNRG RCVENECVCDEGFTGEDCSELICPNDCFDRGRCINGTCYCEEGFTGEDCGKPTCPHACH TQGRCEEGQCVCDEGFAGVDCSEKRCPADCHNRGRCVDGRCECDDGFTGADCGELKC PNGCSGHGRCVNGQCVCDEGYTGEDCSQLRCPNDCHSRGRCVEGKCVCEQGFKGYDC SDMSCPNDCHQHGRCVNGMCVCDDGYTGEDCRDRQCPRDCSNRGLCVDGQCVCEDG FTGPDCAELSCPNDCHGRGRCVNGQCVCHEGFMGKDCKEQRCPSDCHGQGRCVDGQ CICHEGFTGLDCGQHSCPSDCNNLGQCVSGRCICNEGYSGEDCSEVSPPKDLVVTEVTE ETVNLAWDNEMRVTEYLVVYTPTHEGGLEMQFRVPGDQTSTIIQELEPGVEYFIRVFAI LENKKSIPVSARVATYLPAPEGLKFKSIKETSVEVEWDPLDIAFETWEIIFRNMNKEDEG EITKSLRRPETSYRQTGLAPGQEYEISLHIVKNNTRGPGLKRVTTTRLDAPSQIEVKDVT DTTALITWFKPLAEIDGIELTYGIKDVPGDRTTIDLTEDENQYSIGNLKPDTEYEVSLISRR GDMSSNPAKETFTTGLDAPRNLRRVSQTDNSITLEWRNGKAAIDSYRIKYAPISGGDHA EVDVPKSQQATTKTTLTGLRPGTEYGIGVSAVKEDKESNPATINAATELDTPKDLQVSE TAETSLTLLWKTPLAKFDRYRLNYSLPTGQWVGVQLPRNTTSYVLRGLEPGQEYNVLL TAEKGRHKSKPARVKASTEQAPELENLTVTEVGWDGLRLNWTAADQAYEHFIIQVQE ANKVEAARNLTVPGSLRAVDIPGLKAATPYTVSIYGVIQGYRTPVLSAEASTGETPNLG EVVVAEVGWDALKLNWTAPEGAYEYFFIQVQEADTVEAAQNLTVPGGLRSTDLPGLK AATHYTITIRGVTQDFSTTPLSVEVLTEEVPDMGNLTVTEVSWDALRLNWTTPDGTYD QFTIQVQEADQVEEAHNLTVPGSLRSMEIPGLRAGTPYTVTLHGEVRGHSTRPLAVEVV TEDLPQLGDLAVSEVGWDGLRLNWTAADNAYEHFVIQVQEVNKVEAAQNLTLPGSLR AVDIPGLEAATPYRVSIYGVIRGYRTPVLSAEASTAKEPEIGNLNVSDITPESFNLSWMA TDGIFETFTIEIIDSNRLLETVEYNISGAERTAHISGLPPSTDFIVYLSGLAPSIRTKTISATA TTEALPLLENLTISDINPYGFTVSWMASENAFDSFLVTVVDSGKLLDPQEFTLSGTQRKL ELRGLITGIGYEVMVSGFTQGHQTKPLRAEIVTEAEPEVDNLLVSDATPDGFRLSWTAD EGVFDNFVLKIRDTKKQSEPLEITLLAPERTRDLTGLREATEYEIELYGISKGRRSQTVSA IATTAMGSPKEVIFSDITENSATVSWRAPTAQVESFRITYVPITGGTPSMVTVDGTKTQT RLVKLIPGVEYLVSIIAMKGFEESEPVSGSFTTALDGPSGLVTANITDSEALARWQPAIAT VDSYVISYTGEKVPEITRTVSGNTVEYALTDLEPATEYTLRIFAEKGPQKSSTITAKFTTD LDSPRDLTATEVQSETALLTWRPPRASVTGYLLVYESVDGTVKEVIVGPDTTSYSLADL SPSTHYTAKIQALNGPLRSNMIQTIFTTIGLLYPFPKDCSQAMLNGDTTSGLYTIYLNGD KAQALEVFCDMTSDGGGWIV corresponding to amino acids 1-2025 of TENA_HUMAN_V1, which also corresponds to amino acids 1-2025 of HUMTEN_PEA_(—)1_P14 (SEQ ID NO: 941), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence STTRDCRALRPRGRGRGQSRGGEEGDLLLMHSDTPMCEALQDSACHTEALRNSLLNKR MGNTLATF (SEQ ID NO: 1100) corresponding to amino acids 2026-2091 of HUMTEN_PEA_(—)1_P14 (SEQ ID NO: 941), wherein said first amino acid sequence and second amino acid sequence are contiguous and in a sequential order.

2. An isolated polypeptide encoding for a tail of HUMTEN_PEA_(—)1_P14 (SEQ ID NO: 941), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence STTRDCRALRPRGRGRGQSRGGEEGDLLLMHSDTPMCEALQDSACHTEALRNSLLNKR MGNTLATF (SEQ ID NO: 1100) in HUMTEN_PEA_(—)1_P14 (SEQ ID NO: 941).

It should be noted that the known protein sequence (TENA_HUMAN) has one or more changes than the sequence given at the end of the application and named as being the amino acid sequence for TENA_HUMAN_V1. These changes were previously known to occur and are listed in the table below.

TABLE 28 Changes to TENA_HUMAN_V1 SNP position(s) on amino acid sequence Type of change 371 conflict 540 conflict

The location of the variant protein was determined according to results from a number of different software programs and analyses, including analyses from SignalP and other specialized programs. The variant protein is believed to be located as follows with regard to the cell: secreted. The protein localization is believed to be secreted because of manual inspection of known protein localization and/or gene structure.

Variant protein HUMTEN_PEA_(—)1_P14 (SEQ ID NO: 941) also has the following non-silent SNPs (Single Nucleotide Polymorphisms) as listed in Table 29, (given according to their position(s) on the amino acid sequence, with the alternative amino acid(s) listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein HUMTEN_PEA_(—)1_P14 (SEQ ID NO: 941) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 29 Amino acid mutations SNP position(s) on amino acid sequence Alternative amino acid(s) Previously known SNP? 149 Q -> * No 213 G -> S Yes 370 V -> L Yes 539 R -> Q Yes 605 V -> I Yes 680 Q -> R Yes 842 V -> L No 850 D -> H Yes 851 L -> V Yes 1066 R -> H No 1677 L -> I Yes 1781 A -> T Yes 2008 Q -> E Yes 2060 P -> H Yes

Variant protein HUMTEN_PEA_(—)1_P14 (SEQ ID NO: 941) is encoded by the following transcript(s): HUMTEN_PEA_(—)1_T17 (SEQ ID NO: 864), for which the sequence(s) is/are given at the end of the application. The coding portion of transcript HUMTEN_PEA_(—)1_T17 (SEQ ID NO: 864) is shown in bold; this coding portion starts at position 348 and ends at position 6620. The transcript also has the following SNPs as listed in Table 30 (given according to their position on the nucleotide sequence, with the alternative nucleic acid listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein HUMTEN_PEA_(—)1_P14 (SEQ ID NO: 941) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 30 Nucleic acid SNPs SNP position on nucleotide sequence Alternative nucleic acid Previously known SNP? 115 T -> G Yes 123 A -> G Yes 315 C -> T Yes 434 C -> T Yes 503 C -> T Yes 542 G -> A Yes 623 A -> G Yes 792 C -> T No 984 G -> A Yes 1043 A -> G Yes 1455 G -> T Yes 1963 G -> A Yes 2156 A -> G Yes 2160 G -> A Yes 2386 A -> G Yes 2396 A -> G Yes 2654 G -> A No 2871 G -> T No 2895 G -> C Yes 2898 C -> G Yes 3005 A -> G No 3512 C -> T Yes 3544 G -> A No 3635 A -> G Yes 4922 G -> A No 5376 T -> A Yes 5549 A -> G Yes 5688 G -> A Yes 6020 A -> G Yes 6092 C -> A Yes 6369 C -> G Yes 6526 C -> A Yes 6678 C -> G Yes 7233 A -> No 7257 A -> No 7345 G -> No 7393 C -> T Yes 7663 G -> No 7739 A -> No 7739 A -> C No 7754 C -> G Yes 7755 C -> G Yes 7757 G -> No 7865 G -> A Yes 7945 A -> G No 8153 A -> C No 8159 T -> C No 8180 -> T No 8349 -> T No 8360 A -> C No 8704 G -> C Yes 9266 G -> T Yes

Variant protein HUMTEN_PEA_(—)1_P15 (SEQ ID NO: 942) according to the present invention has an amino acid sequence as given at the end of the application; it is encoded by transcript(s) HUMTEN_PEA_(—)1_T18 (SEQ ID NO: 865). An alignment is given to the known protein (Tenascin precursor) at the end of the application. One or more alignments to one or more previously published protein sequences are given at the end of the application. A brief description of the relationship of the variant protein according to the present invention to each such aligned protein is as follows:

Comparison report between HUMTEN_PEA_(—)1_P15 (SEQ ID NO: 942) and TENA_HUMAN_V1:

1. An isolated chimeric polypeptide encoding for HUMTEN_PEA_(—)1_P15 (SEQ ID NO: 942), comprising a first amino acid sequence being at least 90% homologous to MGAMTQLLAGVFLAFLALATEGGVLKKVIRHKRQSGVNATLPEENQPVVFNHVYNIK LPVGSQCSVDLESASGEKDLAPPSEPSESFQEHTVDGENQIVFTHRINIPRRACGCAAAP DVKELLSRLEELENLVSSLREQCTAGAGCCLQPATGRLDTRPFCSGRGNFSTEGCGCVC EPGWKGPNCSEPECPGNCHLRGRCIDGQCICDDGFTGEDCSQLACPSDCNDQGKCVNG VCICFEGYAGADCSREICPVPCSEEHGTCVDGLCVCHDGFAGDDCNKPLCLNNCYNRG RCVENECVCDEGFTGEDCSELICPNDCFDRGRCINGTCYCEEGFTGEDCGKPTCPHACH TQGRCEEGQCVCDEGFAGVDCSEKRCPADCHNRGRCVDGRCECDDGFTGADCGELKC PNGCSGHGRCVNGQCVCDEGYTGEDCSQLRCPNDCHSRGRCVEGKCVCEQGFKGYDC SDMSCPNDCHQHGRCVNGMCVCDDGYTGEDCRDRQCPRDCSNRGLCVDGQCVCEDG FTGPDCAELSCPNDCHGRGRCVNGQCVCHEGFMGKDCKEQRCPSDCHGQGRCVDGQ CICHEGFTGLDCGQHSCPSDCNNLGQCVSGRCICNEGYSGEDCSEVSPPKDLVVTEVTE ETVNLAWDNEMRVTEYLVVYTPTHEGGLEMQFRVPGDQTSTIIQELEPGVEYFIRVFAI LENKKSIPVSARVATYLPAPEGLKFKSIKETSVEVEWDPLDIAFETWEIIFRNMNKEDEG EITKSLRRPETSYRQTGLAPGQEYEISLHIVKNNTRGPGLKRVTTTRLDAPSQIEVKDVT DTTALITWFKPLAEIDGIELTYGIKDVPGDRTTIDLTEDENQYSIGNLKPDTEYEVSLISRR GDMSSNPAKETFTTGLDAPRNLRRVSQTDNSITLEWRNGKAAIDSYRIKYAPISGGDHA EVDVPKSQQATTKTTLTGLRPGTEYGIGVSAVKEDKESNPATINAATELDTPKDLQVSE TAETSLTLLWKTPLAKFDRYRLNYSLPTGQWVGVQLPRNTTSYVLRGLEPGQEYNVLL TAEKGRHKSKPARVKAS corresponding to amino acids 1-1070 of TENA_HUMAN_V1, which also corresponds to amino acids 1-1070 of HUMTEN_PEA_(—)1_P15 (SEQ ID NO: 942), and a second amino acid sequence being at least 90% homologous to TEAEPEVDNLLVSDATPDGFRLSWTADEGVFDNFVLKIRDTKKQSEPLEITLLAPERTRD LTGLREATEYEIELYGISKGRRSQTVSAIATTAMGSPKEVIFSDITENSATVSWRAPTAQV ESFRITYVPITGGTPSMVTVDGTKTQTRLVKLIPGVEYLVSIIAMKGFEESEPVSGSFTTA LDGPSGLVTANITDSEALARWQPAIATVDSYVISYTGEKVPEITRTVSGNTVEYALTDLE PATEYTLRIFAEKGPQKSSTITAKFTTDLDSPRDLTATEVQSETALLTWRPPRASVTGYL LVYESVDGTVKEVIVGPDTTSYSLADLSPSTHYTAKIQALNGPLRSNMIQTIFTTIGLLYP FPKDCSQAMLNGDTTSGLYTIYLNGDKAQALEVFCDMTSDGGGWIVFLRRKNGRENF YQNWKAYAAGFGDRREEFWLGLDNLNKITAQGQYELRVDLRDHGETAFAVYDKFSV GDAKTRYKLKVEGYSGTAGDSMAYHNGRSFSTFDKDTDSAITNCALSYKGAFWYRNC HRVNLMGRYGDNNHSQGVNWFHWKGHEHSIQFAEMKLRPSNFRNLEGRRKRA corresponding to amino acids 1617-2201 of TENA_HUMAN_V1, which also corresponds to amino acids 1071-1655 of HUMTEN_PEA_(—)1_P15 (SEQ ID NO: 942), wherein said first amino acid sequence and second amino acid sequence are contiguous and in a sequential order.

2. An isolated chimeric polypeptide encoding for an edge portion of HUMTEN_PEA_(—)1_P15 (SEQ ID NO: 942), comprising a polypeptide having a length “n”, wherein n is at least about 10 amino acids in length, optionally at least about 20 amino acids in length, preferably at least about 30 amino acids in length, more preferably at least about 40 amino acids in length and most preferably at least about 50 amino acids in length, wherein at least two amino acids comprise ST, having a structure as follows: a sequence starting from any of amino acid numbers 1070-x to 1070; and ending at any of amino acid numbers 1071+((n−2)-x), in which x varies from 0 to n−2.

It should be noted that the known protein sequence (TENA_HUMAN) has one or more changes than the sequence given at the end of the application and named as being the amino acid sequence for TENA_HUMAN_V1. These changes were previously known to occur and are listed in the table below.

TABLE 31 Changes to TENA_HUMAN_V1 SNP position(s) on amino acid sequence Type of change 371 conflict 540 conflict

The location of the variant protein was determined according to results from a number of different software programs and analyses, including analyses from SignalP and other specialized programs. The variant protein is believed to be located as follows with regard to the cell: secreted. The protein localization is believed to be secreted because both signal-peptide prediction programs predict that this protein has a signal peptide, and neither trans-membrane region prediction program predicts that this protein has a trans-membrane region.

Variant protein HUMTEN_PEA_(—)1_P15 (SEQ ID NO: 942) also has the following non-silent SNPs (Single Nucleotide Polymorphisms) as listed in Table 32, (given according to their position(s) on the amino acid sequence, with the alternative amino acid(s) listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein HUMTEN_PEA_(—)1_P15 (SEQ ID NO: 942) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 32 Amino acid mutations SNP position(s) on amino acid sequence Alternative amino acid(s) Previously known SNP? 149 Q -> * No 213 G -> S Yes 370 V -> L Yes 539 R -> Q Yes 605 V -> I Yes 680 Q -> R Yes 842 V -> L No 850 D -> H Yes 851 L -> V Yes 1066 R -> H No 1131 L -> I Yes 1235 A -> T Yes 1462 Q -> E Yes 1484 K -> No 1492 Q -> No 1521 Q -> No 1627 K -> No 1653 K -> No 1653 K -> Q No

Variant protein HUMTEN_PEA_(—)1_P15 (SEQ ID NO: 942) is encoded by the following transcript(s): HUMTEN_PEA_(—)1_T18 (SEQ ID NO: 865), for which the sequence(s) is/are given at the end of the application. The coding portion of transcript HUMTEN_PEA_(—)1_T18 (SEQ ID NO: 865) is shown in bold; this coding portion starts at position 348 and ends at position 5312. The transcript also has the following SNPs as listed in Table 33 (given according to their position on the nucleotide sequence, with the alternative nucleic acid listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein HUMTEN_PEA_(—)1_P15 (SEQ ID NO: 942) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 33 Nucleic acid SNPs SNP position on nucleotide sequence Alternative nucleic acid Previously known SNP? 115 T -> G Yes 123 A -> G Yes 315 C -> T Yes 434 C -> T Yes 503 C -> T Yes 542 G -> A Yes 623 A -> G Yes 792 C -> T No 984 G -> A Yes 1043 A -> G Yes 1455 G -> T Yes 1963 G -> A Yes 2156 A -> G Yes 2160 G -> A Yes 2386 A -> G Yes 2396 A -> G Yes 2654 G -> A No 2871 G -> T No 2895 G -> C Yes 2898 C -> G Yes 3005 A -> G No 3512 C -> T Yes 3544 G -> A No 3738 T -> A Yes 3911 A -> G Yes 4050 G -> A Yes 4382 A -> G Yes 4454 C -> A Yes 4731 C -> G Yes 4798 A -> No 4822 A -> No 4910 G -> No 4958 C -> T Yes 5228 G -> No 5304 A -> No 5304 A -> C No 5319 C -> G Yes 5320 C -> G Yes 5322 G -> No 5430 G -> A Yes 5510 A -> G No 5718 A -> C No 5724 T -> C No 5745 -> T No 5914 -> T No 5925 A -> C No 6269 G -> C Yes 6831 G -> T Yes

Variant protein HUMTEN_PEA_(—)1_P16 (SEQ ID NO: 943) according to the present invention has an amino acid sequence as given at the end of the application; it is encoded by transcript(s) HUMTEN_PEA_(—)1_T19 (SEQ ID NO: 866). An alignment is given to the known protein (Tenascin precursor) at the end of the application. One or more alignments to one or more previously published protein sequences are given at the end of the application. A brief description of the relationship of the variant protein according to the present invention to each such aligned protein is as follows:

Comparison report between HUMTEN_PEA_(—)1_P16 (SEQ ID NO: 943) and TENA_HUMAN_V1:

1. An isolated chimeric polypeptide encoding for HUMTEN_PEA_(—)1_P16 (SEQ ID NO: 943), comprising a first amino acid sequence being at least 90% homologous to MGAMTQLLAGVFLAFLALATEGGVLKKVIRHKRQSGVNATLPEENQPVVFNHVYNIK LPVGSQCSVDLESASGEKDLAPPSEPSESFQEHTVDGENQIVFTHRINIPRRACGCAAAP DVKELLSRLEELENLVSSLREQCTAGAGCCLQPATGRLDTRPFCSGRGNFSTEGCGCVC EPGWKGPNCSEPECPGNCHLRGRCIDGQCICDDGFTGEDCSQLACPSDCNDQGKCVNG VCICFEGYAGADCSREICPVPCSEEHGTCVDGLCVCHDGFAGDDCNKPLCLNNCYNRG RCVENECVCDEGFTGEDCSELICPNDCFDRGRCINGTCYCEEGFTGEDCGKPTCPHACH TQGRCEEGQCVCDEGFAGVDCSEKRCPADCHNRGRCVDGRCECDDGFTGADCGELKC PNGCSGHGRCVNGQCVCDEGYTGEDCSQLRCPNDCHSRGRCVEGKCVCEQGFKGYDC SDMSCPNDCHQHGRCVNGMCVCDDGYTGEDCRDRQCPRDCSNRGLCVDGQCVCEDG FTGPDCAELSCPNDCHGRGRCVNGQCVCHEGFMGKDCKEQRCPSDCHGQGRCVDGQ CICHEGFTGLDCGQHSCPSDCNNLGQCVSGRCICNEGYSGEDCSEVSPPKDLVVTEVTE ETVNLAWDNEMRVTEYLVVYTPTHEGGLEMQFRVPGDQTSTIIQELEPGVEYFIRVFAI LENKKSIPVSARVATYLPAPEGLKFKSIKETSVEVEWDPLDIAFETWEIIFRNMNKEDEG EITKSLRRPETSYRQTGLAPGQEYEISLHIVKNNTRGPGLKRVTTTRLDAPSQIEVKDVT DTTALITWFKPLAEIDGIELTYGIKDVPGDRTTIDLTEDENQYSIGNLKPDTEYEVSLISRR GDMSSNPAKETFTTGLDAPRNLRRVSQTDNSITLEWRNGKAAIDSYRIKYAPISGGDHA EVDVPKSQQATTKTTLTGLRPGTEYGIGVSAVKEDKESNPATINAATELDTPKDLQVSE TAETSLTLLWKTPLAKFDRYRLNYSLPTGQWVGVQLPRNTTSYVLRGLEPGQEYNVLL TAEKGRHKSKPARVKAS corresponding to amino acids 1-1070 of TENA_HUMAN_V1, which also corresponds to amino acids 1-1070 of HUMTEN_PEA_(—)1_P16 (SEQ ID NO: 943), and a second amino acid sequence being at least 90% homologous to TAMGSPKEVIFSDITENSATVSWRAPTAQVESFRITYVPITGGTPSMVTVDGTKTQTRLV KLIPGVEYLVSIIAMKGFEESEPVSGSFTTALDGPSGLVTANITDSEALARWQPAIATVDS YVISYTGEKVPEITRTVSGNTVEYALTDLEPATEYTLRIFAEKGPQKSSTITAKFTTDLDS PRDLTATEVQSETALLTWRPPRASVTGYLLVYESVDGTVKEVIVGPDTTSYSLADLSPS THYTAKIQALNGPLRSNMIQTIFTTIGLLYPFPKDCSQAMLNGDTTSGLYTIYLNGDKAQ ALEVFCDMTSDGGGWIVFLRRKNGRENFYQNWKAYAAGFGDRREEFWLGLDNLNKIT AQGQYELRVDLRDHGETAFAVYDKFSVGDAKTRYKLKVEGYSGTAGDSMAYHNGRS FSTFDKDTDSAITNCALSYKGAFWYRNCHRVNLMGRYGDNNHSQGVNWFHWKGHEH SIQFAEMKLRPSNFRNLEGRRKRA corresponding to amino acids 1708-2201 of TENA_HUMAN_V1, which also corresponds to amino acids 1071-1564 of HUMTEN_PEA_(—)1_P16 (SEQ ID NO: 943), wherein said first amino acid sequence and second amino acid sequence are contiguous and in a sequential order.

2. An isolated chimeric polypeptide encoding for an edge portion of HUMTEN_PEA_(—)1_P16 (SEQ ID NO: 943), comprising a polypeptide having a length “n”, wherein n is at least about 10 amino acids in length, optionally at least about 20 amino acids in length, preferably at least about 30 amino acids in length, more preferably at least about 40 amino acids in length and most preferably at least about 50 amino acids in length, wherein at least two amino acids comprise ST, having a structure as follows: a sequence starting from any of amino acid numbers 1070-x to 1070; and ending at any of amino acid numbers 1071+((n−2)-x), in which x varies from 0 to n−2.

It should be noted that the known protein sequence (TENA_HUMAN) has one or more changes than the sequence given at the end of the application and named as being the amino acid sequence for TENA_HUMAN_V1. These changes were previously known to occur and are listed in the table below.

TABLE 34 Changes to TENA_HUMAN_V1 SNP position(s) on amino acid sequence Type of change 371 conflict 540 conflict

The location of the variant protein was determined according to results from a number of different software programs and analyses, including analyses from SignalP and other specialized programs. The variant protein is believed to be located as follows with regard to the cell: secreted. The protein localization is believed to be secreted because of manual inspection of known protein localization and/or gene structure.

Variant protein HUMTEN_PEA_(—)1_P16 (SEQ ID NO: 943) also has the following non-silent SNPs (Single Nucleotide Polymorphisms) as listed in Table 35, (given according to their position(s) on the amino acid sequence, with the alternative amino acid(s) listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein HUMTEN_PEA_(—)1_P16 (SEQ ID NO: 943) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 35 Amino acid mutations SNP position(s) on amino acid sequence Alternative amino acid(s) Previously known SNP? 149 Q -> * No 213 G -> S Yes 370 V -> L Yes 539 R -> Q Yes 605 V -> I Yes 680 Q -> R Yes 842 V -> L No 850 D -> H Yes 851 L -> V Yes 1066 R -> H No 1144 A -> T Yes 1371 Q -> E Yes 1393 K -> No 1401 Q -> No 1430 Q -> No 1536 K -> No 1562 K -> No 1562 K -> Q No

Variant protein HUMTEN_PEA_(—)1_P16 (SEQ ID NO: 943) is encoded by the following transcript(s): HUMTEN_PEA_(—)1_T19 (SEQ ID NO: 866), for which the sequence(s) is/are given at the end of the application. The coding portion of transcript HUMTEN_PEA_(—)1_T19 (SEQ ID NO: 866) is shown in bold; this coding portion starts at position 348 and ends at position 5039. The transcript also has the following SNPs as listed in Table 36 (given according to their position on the nucleotide sequence, with the alternative nucleic acid listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein HUMTEN_PEA_(—)1_P16 (SEQ ID NO: 943) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 36 Nucleic acid SNPs SNP position on nucleotide sequence Alternative nucleic acid Previously known SNP? 115 T -> G Yes 123 A -> G Yes 315 C -> T Yes 434 C -> T Yes 503 C -> T Yes 542 G -> A Yes 623 A -> G Yes 792 C -> T No 984 G -> A Yes 1043 A -> G Yes 1455 G -> T Yes 1963 G -> A Yes 2156 A -> G Yes 2160 G -> A Yes 2386 A -> G Yes 2396 A -> G Yes 2654 G -> A No 2871 G -> T No 2895 G -> C Yes 2898 C -> G Yes 3005 A -> G No 3512 C -> T Yes 3544 G -> A No 3638 A -> G Yes 3777 G -> A Yes 4109 A -> G Yes 4181 C -> A Yes 4458 C -> G Yes 4525 A -> No 4549 A -> No 4637 G -> No 4685 C -> T Yes 4955 G -> No 5031 A -> No 5031 A -> C No 5046 C -> G Yes 5047 C -> G Yes 5049 G -> No 5157 G -> A Yes 5237 A -> G No 5445 A -> C No 5451 T -> C No 5472 -> T No 5641 -> T No 5652 A -> C No 5996 G -> C Yes 6558 G -> T Yes

Variant protein HUMTEN_PEA_(—)1_P17 (SEQ ID NO: 944) according to the present invention has an amino acid sequence as given at the end of the application; it is encoded by transcript(s) HUMTEN_PEA_(—)1_T20 (SEQ ID NO. 867). An alignment is given to the known protein (Tenascin precursor) at the end of the application. One or more alignments to one or more previously published protein sequences are given at the end of the application. A brief description of the relationship of the variant protein according to the present invention to each such aligned protein is as follows:

Comparison report between HUMTEN_PEA_(—)1_P17 (SEQ ID NO: 944) and TENA_HUMAN_V1:

1. An isolated chimeric polypeptide encoding for HUMTEN_PEA_(—)1_P17 (SEQ ID NO: 944), comprising a first amino acid sequence being at least 90% homologous to MGAMTQLLAGVFLAFLALATEGGVLKKVIRHKRQSGVNATLPEENQPVVFNHVYNIK LPVGSQCSVDLESASGEKDLAPPSEPSESFQEHTVDGENQIVFTHRINIPRRACGCAAAP DVKELLSRLEELENLVSSLREQCTAGAGCCLQPATGRLDTRPFCSGRGNFSTEGCGCVC EPGWKGPNCSEPECPGNCHLRGRCIDGQCICDDGFTGEDCSQLACPSDCNDQGKCVNG VCICFEGYAGADCSREICPVPCSEEHGTCVDGLCVCHDGFAGDDCNKPLCLNNCYNRG RCVENECVCDEGFTGEDCSELICPNDCFDRGRCINGTCYCEEGFTGEDCGKPTCPHACH TQGRCEEGQCVCDEGFAGVDCSEKRCPADCHNRGRCVDGRCECDDGFTGADCGELKC PNGCSGHGRCVNGQCVCDEGYTGEDCSQLRCPNDCHSRGRCVEGKCVCEQGFKGYDC SDMSCPNDCHQHGRCVNGMCVCDDGYTGEDCRDRQCPRDCSNRGLCVDGQCVCEDG FTGPDCAELSCPNDCHGRGRCVNGQCVCHEGFMGKDCKEQRCPSDCHGQGRCVDGQ CICHEGFTGLDCGQHSCPSDCNNLGQCVSGRCICNEGYSGEDCSEVSPPKDLVVTEVTE ETVNLAWDNEMRVTEYLVVYTPTHEGGLEMQFRVPGDQTSTIIQELEPGVEYFIRVFAI LENKKSIPVSARVATYLPAPEGLKFKSIKETSVEVEWDPLDIAFETWEIIFRNMNKEDEG EITKSLRRPETSYRQTGLAPGQEYEISLHIVKNNTRGPGLKRVTTTRLDAPSQIEVKDVT DTTALITWFKPLAEIDGIELTYGIKDVPGDRTTIDLTEDENQYSIGNLKPDTEYEVSLISRR GDMSSNPAKETFTTGLDAPRNLRRVSQTDNSITLEWRNGKAAIDSYRIKYAPISGGDHA EVDVPKSQQATTKTTLTGLRPGTEYGIGVSAVKEDKESNPATINAATELDTPKDLQVSE TAETSLTLLWKTPLAKFDRYRLNYSLPTGQWVGVQLPRNTTSYVLRGLEPGQEYNVLL TAEKGRHKSKPARVKASTEQAPELENLTVTEVGWDGLRLNWTAADQAYEHFIIQVQE ANKVEAARNLTVPGSLRAVDIPGLKAATPYTVSIYGVIQGYRTPVLSAEASTGETPNLG EVVVAEVGWDALKLNWTAPEGAYEYFFIQVQEADTVEAAQNLTVPGGLRSTDLPGLK AATHYTITIRGVTQDFSTTPLSVEVLTEEVPDMGNLTVTEVSWDALRLNWTTPDGTYD QFTIQVQEADQVEEAHNLTVPGSLRSMEIPGLRAGTPYTVTLHGEVRGHSTRPLAVEVV TEDLPQLGDLAVSEVGWDGLRLNWTAADNAYEHFVIQVQEVNKVEAAQNLTLPGSLR AVDIPGLEAATPYRVSIYGVIRGYRTPVLSAEASTAKEPEIGNLNVSDITPESFNLSWMA TDGIFETFTIEIIDSNRLLETVEYNISGAERTAHISGLPPSTDFIVYLSGLAPSIRTKTISATA TTEALPLLENLTISDINPYGFTVSWMASENAFDSFLVTVVDSGKLLDPQEFTLSGTQRKL ELRGLITGIGYEVMVSGFTQGHQTKPLRAEIVTEAEPEVDNLLVSDATPDGFRLSWTAD EGVFDNFVLKIRDTKKQSEPLEITLLAPERTRDLTGLREATEYEIELYGISKGRRSQTVSA IATTAMGSPKEVIFSDITENSATVSWRAPTAQVESFRITYVPITGGTPSMVTVDGTKTQT RLVKLIPGVEYLVSIIAMKGFEESEPVSGSFTTALDGPSGLVTANITDSEALARWQPAIAT VDSYVISYTGEKVPEITRTVSGNTVEYALTDLEPATEYTLRIFAEKGPQKSSTITAKFTTD LDSPRDLTATEVQSETALLTWRPPRASVTGYLLVYESVDGTVKEVIVGPDTTSYSLADL SPSTHYTAKIQALNGPLRSNMIQTIFTTIGLLYPFPKDCSQAMLNGDTTSGLYTIYLNGD KAQALEVFCDMTSDGGGWIV corresponding to amino acids 1-2025 of TENA_HUMAN_V1, which also corresponds to amino acids 1-2025 of HUMTEN_PEA_(—)1_P17 (SEQ ID NO: 944), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence TPWPTTMADPSPPLTRTQIQPSPTVLCPTKGLSGTGTVTVST (SEQ ID NO: 1101) corresponding to amino acids 2026-2067 of HUMTEN_PEA_(—)1_P17 (SEQ ID NO: 944), wherein said first amino acid sequence and second amino acid sequence are contiguous and in a sequential order.

2. An isolated polypeptide encoding for a tail of HUMTEN_PEA_(—)1_P17 (SEQ ID NO: 944), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence TPWPTTMADPSPPLTRTQIQPSPTVLCPTKGLSGTGTVTVST (SEQ ID NO: 1101) in HUMTEN_PEA_(—)1_T17 (SEQ ID NO: 944).

It should be noted that the known protein sequence (TENA_HUMAN) has one or more changes than the sequence given at the end of the application and named as being the amino acid sequence for TENA_HUMAN_V1. These changes were previously known to occur and are listed in the table below.

TABLE 37 Changes to TENA_HUMAN_V1 SNP positions(s) on amino acid sequence Type of change 371 conflict 540 conflict

The location of the variant protein was determined according to results from a number of different software programs and analyses, including analyses from SignalP and other specialized programs. The variant protein is believed to be located as follows with regard to the cell: secreted. The protein localization is believed to be secreted because of manual inspection of known protein localization and/or gene structure.

Variant protein HUMTEN_PEA_(—)1_P17 (SEQ ID NO: 944) also has the following non-silent SNPs (Single Nucleotide Polymorphisms) as listed in Table 38, (given according to their position(s) on the amino acid sequence, with the alternative amino acid(s) listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein HUMTEN_PEA_(—)1_P17 (SEQ ID NO: 944) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 38 Amino acid mutations SNP position(s) on amino acid sequence Alternatvie amino acid(s) Previously known SNP? 149 Q -> * No 213 G -> S Yes 370 V -> L Yes 539 R -> Q Yes 605 V -> I Yes 680 Q -> R Yes 842 V -> L No 850 D -> H Yes 851 L -> V Yes 1066 R -> H No 1677 L -> I Yes 1781 A -> T Yes 2008 Q -> E Yes

Variant protein HUMTEN_PEA_(—)1_P17 (SEQ ID NO: 944) is encoded by the following transcript(s): HUMTEN_PEA_(—)1_T20 (SEQ ID NO. 867), for which the sequence(s) is/are given at the end of the application. The coding portion of transcript HUMTEN_PEA_(—)1_T20 (SEQ ID NO. 867) is shown in bold; this coding portion starts at position 348 and ends at position 6548. The transcript also has the following SNPs as listed in Table 39 (given according to their position on the nucleotide sequence, with the alternative nucleic acid listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein HUMTEN_PEA_(—)1_P17 (SEQ ID NO: 944) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 39 Nucleic acid SNPs SNP position on nucleotide sequence Alternative nucleic acid Previously known SNP? 115 T -> G Yes 123 A -> G Yes 315 C -> T Yes 434 C -> T Yes 503 C -> T Yes 542 G -> A Yes 623 A -> G Yes 792 C -> T No 984 G -> A Yes 1043 A -> G Yes 1455 G -> T Yes 1963 G -> A Yes 2156 A -> G Yes 2160 G -> A Yes 2386 A -> G Yes 2396 A -> G Yes 2654 G -> A No 2871 G -> T No 2895 G -> C Yes 2898 C -> G Yes 3005 A -> G No 3512 C -> T Yes 3544 G -> A No 3635 A -> G Yes 4922 G -> A No 5376 T -> A Yes 5549 A -> G Yes 5688 G -> A Yes 6020 A -> G Yes 6092 C -> A Yes 6369 C -> G Yes 6607 G -> No 6683 A -> No 6683 A -> C No 6698 C -> G Yes 6699 C -> G Yes 6701 G -> No 6809 G -> A Yes 6889 A -> G No 7097 A -> C No 7103 T -> C No 7124 -> T No 7293 -> T No 7304 A -> C No 7648 G -> C Yes 8210 G -> T Yes

Variant protein HUMTEN_PEA_(—)1_P20 (SEQ ID NO: 945) according to the present invention has an amino acid sequence as given at the end of the application; it is encoded by transcript(s) HUMTEN_PEA_(—)1_T23 (SEQ ID NO: 868). An alignment is given to the known protein (Tenascin precursor) at the end of the application. One or more alignments to one or more previously published protein sequences are given at the end of the application. A brief description of the relationship of the variant protein according to the present invention to each such aligned protein is as follows:

Comparison report between HUMTEN_PEA_(—)1_P20 (SEQ ID NO: 945) and TENA_HUMAN_V1:

1. An isolated chimeric polypeptide encoding for HUMTEN_PEA_(—)1_P20 (SEQ ID NO: 945), comprising a first amino acid sequence being at least 90% homologous to MGAMTQLLAGVFLAFLALATEGGVLKKVIRHKRQSGVNATLPEENQPVVFNHVYNIK LPVGSQCSVDLESASGEKDLAPPSEPSESFQEHTVDGENQIVFTHRINIPRRACGCAAAP DVKELLSRLEELENLVSSLREQCTAGAGCCLQPATGRLDTRPFCSGRGNFSTEGCGCVC EPGWKGPNCSEPECPGNCHLRGRCIDGQCICDDGFTGEDCSQLACPSDCNDQGKCVNG VCICFEGYAGADCSREICPVPCSEEHGTCVDGLCVCHDGFAGDDCNKPLCLNNCYNRG RCVENECVCDEGFTGEDCSELICPNDCFDRGRCINGTCYCEEGFTGEDCGKPTCPHACH TQGRCEEGQCVCDEGFAGVDCSEKRCPADCHNRGRCVDGRCECDDGFTGADCGELKC PNGCSGHGRCVNGQCVCDEGYTGEDCSQLRCPNDCHSRGRCVEGKCVCEQGFKGYDC SDMSCPNDCHQHGRCVNGMCVCDDGYTGEDCRDRQCPRDCSNRGLCVDGQCVCEDG FTGPDCAELSCPNDCHGRGRCVNGQCVCHEGFMGKDCKEQRCPSDCHGQGRCVDGQ CICHEGFTGLDCGQHSCPSDCNNLGQCVSGRCICNEGYSGEDCSEVSPPKDLVVTEVTE ETVNLAWDNEMRVTEYLVVYTPTHEGGLEMQFRVPGDQTSTIIQELEPGVEYFIRVFAI LENKKSIPVSARVATYLPAPEGLKFKSIKETSVEVEWDPLDIAFETWEIIFRNMNKEDEG EITKSLRRPETSYRQTGLAPGQEYEISLHIVKNNTRGPGLKRVTTTRLDAPSQIEVKDVT DTTALITWFKPLAEIDGIELTYGIKDVPGDRTTIDLTEDENQYSIGNLKPDTEYEVSLISRR GDMSSNPAKETFTTGLDAPRNLRRVSQTDNSITLEWRNGKAAIDSYRIKYAPISGGDHA EVDVPKSQQATTKTTLTGLRPGTEYGIGVSAVKEDKESNPATINAATELDTPKDLQVSE TAETSLTLLWKTPLAKFDRYRLNYSLPTGQWVGVQLPRNTTSYVLRGLEPGQEYNVLL TAEKGRHKSKPARVKASTEQAPELENLTVTEVGWDGLRLNWTAADQAYEHFIIQVQE ANKVEAARNLTVPGSLRAVDIPGLKAATPYTVSIYGVIQGYRTPVLSAEASTGETPNLG EVVVAEVGWDALKLNWTAPEGAYEYFFIQVQEADTVEAAQNLTVPGGLRSTDLPGLK AATHYTITIRGVTQDFSTTPLSVEVLTEEVPDMGNLTVTEVSWDALRLNWTTPDGTYD QFTIQVQEADQVEEAHNLTVPGSLRSMEIPGLRAGTPYTVTLHGEVRGHSTRPLAVEVV TEDLPQLGDLAVSEVGWDGLRLNWTAADNAYEHFVIQVQEVNKVEAAQNLTLPGSLR AVDIPGLEAATPYRVSIYGVIRGYRTPVLSAEASTAKEPEIGNLNVSDITPESFNLSWMA TDGIFETFTIEIIDSNRLLETVEYNISGAERTAHISGLPPSTDFIVYLSGLAPSIRTKTISATA TTEALPLLENLTISDINPYGFTVSWMASENAFDSFLVTVVDSGKLLDPQEFTLSGTQRKL ELRGLITGIGYEVMVSGFTQGHQTKPLRAEIVTEAEPEVDNLLVSDATPDGFRLSWTAD EGVFDNFVLKIRDTKKQSEPLEITLLAPERTRDLTGLREATEYEIELYGISKGRRSQTVSA IATTAMGSPKEVIFSDITENSATVSWRAPTAQVESFRITYVPITGGTPSMVTVDGTKTQT RLVKLIPGVEYLVSIIAMKGFEESEPVSGSFTTALDGPSGLVTANITDSEALARWQPAIAT VDSYVISYTGEKVPEITRTVSGNTVEYALTDLEPATEYTLRIFAEKGPQKSSTITAKFTTD LDSPRDLTATEVQSETALLTWRPPRASVTGYLLVYESVDGTVKEVIVGPDTTSYSLADL SPSTHYTAKIQALNGPLRSNMIQTIFTTIGLLYPFPKDCSQAMLNGDTTSGLYTIYLNGD KAQALEVFCDMTSDGGGWIVFLRRKNGRENFYQNWKAYAAGFGDRREEFWLG corresponding to amino acids 1-2057 of TENA_HUMAN_V1, which also corresponds to amino acids 1-2057 of HUMTEN_PEA_(—)1_P20 (SEQ ID NO: 945), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence NAALHVYI (SEQ ID NO: 1102) corresponding to amino acids 2058-2065 of HUMTEN_PEA_(—)1_P20 (SEQ ID NO: 945), wherein said first amino acid sequence and second amino acid sequence are contiguous and in a sequential order.

2. An isolated polypeptide encoding for a tail of HUMTEN_PEA_(—)1_P20 (SEQ ID NO: 945), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence NAALHVYI (SEQ ID NO: 1102) in HUMTEN_PEA_(—)1_P20 (SEQ ID NO: 945).

It should be noted that the known protein sequence (TENA_HUMAN) has one or more changes than the sequence given at the end of the application and named as being the amino acid sequence for TENA_HUMAN_V1. These changes were previously known to occur and are listed in the table below.

TABLE 40 Changes to TENA_HUMAN_V1 SNP position(s) on amino acid sequence Type of change 371 conflict 540 conflict

The location of the variant protein was determined according to results from a number of different software programs and analyses, including analyses from SignalP and other specialized programs. The variant protein is believed to be located as follows with regard to the cell: secreted. The protein localization is believed to be secreted because both signal-peptide prediction programs predict that this protein has a signal peptide, and neither trans-membrane region prediction program predicts that this protein has a trans-membrane region.

Variant protein HUMTEN_PEA_(—)1_P20 (SEQ ID NO: 945) also has the following non-silent SNPs (Single Nucleotide Polymorphisms) as listed in Table 41, (given according to their position(s) on the amino acid sequence, with the alternative amino acid(s) listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein HUMTEN_PEA_(—)1_P20 (SEQ ID NO: 945) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 41 Amino acid mutations SNP position(s) on amino acid sequence Alternative amino acid(s) Previously known SNP? 149 Q -> * No 213 G -> S Yes 370 V -> L Yes 539 R -> Q Yes 605 V -> I Yes 680 Q -> R Yes 842 V -> L No 850 D -> H Yes 851 L -> V Yes 1066 R -> H No 1677 L -> I Yes 1781 A -> T Yes 2008 Q -> E Yes 2030 K -> No 2038 Q -> No 2064 Y -> C Yes

Variant protein HUMTEN_PEA_(—)1_P20 (SEQ ID NO: 945) is encoded by the following transcript(s): HUMTEN_PEA_(—)1_T23 (SEQ ID NO: 868), for which the sequence(s) is/are given at the end of the application. The coding portion of transcript HUMTEN_PEA_(—)1_T23 (SEQ ID NO: 868) is shown in bold; this coding portion starts at position 348 and ends at position 6542. The transcript also has the following SNPs as listed in Table 42 (given according to their position on the nucleotide sequence, with the alternative nucleic acid listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein HUMTEN_PEA_(—)1_P20 (SEQ ID NO: 945) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 42 Nucleic acid SNPs SNP position on nucleotide sequence Alternative nucleic acid Previously known SNP? 115 T -> G Yes 123 A -> G Yes 315 C -> T Yes 434 C -> T Yes 503 C -> T Yes 542 G -> A Yes 623 A -> G Yes 792 C -> T No 984 G -> A Yes 1043 A -> G Yes 1455 G -> T Yes 1963 G -> A Yes 2156 A -> G Yes 2160 G -> A Yes 2386 A -> G Yes 2396 A -> G Yes 2654 G -> A No 2871 G -> T No 2895 G -> C Yes 2898 C -> G Yes 3005 A -> G No 3512 C -> T Yes 3544 G -> A No 3635 A -> G Yes 4922 G -> A No 5376 T -> A Yes 5549 A -> G Yes 5688 G -> A Yes 6020 A -> G Yes 6092 C -> A Yes 6369 C -> G Yes 6436 A -> No 6460 A -> No 6538 A -> G Yes 6718 C -> T Yes 6837 T -> G Yes

Variant protein HUMTEN_PEA_(—)1_P26 (SEQ ID NO: 946) according to the present invention has an amino acid sequence as given at the end of the application; it is encoded by transcript(s) HUMTEN_PEA_(—)1_T32 (SEQ ID NO: 869). An alignment is given to the known protein (Tenascin precursor) at the end of the application. One or more alignments to one or more previously published protein sequences are given at the end of the application. A brief description of the relationship of the variant protein according to the present invention to each such aligned protein is as follows:

Comparison report between HUMTEN_PEA_(—)1_P26 (SEQ ID NO: 946) and TENA_HUMAN_V1:

1. An isolated chimeric polypeptide encoding for HUMTEN_PEA_(—)1_P26 (SEQ ID NO: 946), comprising a first amino acid sequence being at least 90% homologous to MGAMTQLLAGVFLAFLALATEGGVLKKVIRHKRQSGVNATLPEENQPVVFNHVYNIK LPVGSQCSVDLESASGEKDLAPPSEPSESFQEHTVDGENQIVFTHRINIPRRACGCAAAP DVKELLSRLEELENLVSSLREQCTAGAGCCLQPATGRLDTRPFCSGRGNFSTEGCGCVC EPGWKGPNCSEPECPGNCHLRGRCIDGQCICDDGFTGEDCSQLACPSDCNDQGKCVNG VCICFEGYAGADCSREICPVPCSEEHGTCVDGLCVCHDGFAGDDCNKPLCLNNCYNRG RCVENECVCDEGFTGEDCSELICPNDCFDRGRCINGTCYCEEGFTGEDCGKPTCPHACH TQGRCEEGQCVCDEGFAGVDCSEKRCPADCHNRGRCVDGRCECDDGFTGADCGELKC PNGCSGHGRCVNGQCVCDEGYTGEDCSQLRCPNDCHSRGRCVEGKCVCEQGFKGYDC SDMSCPNDCHQHGRCVNGMCVCDDGYTGEDCRDRQCPRDCSNRGLCVDGQCVCEDG FTGPDCAELSCPNDCHGRGRCVNGQCVCHEGFMGKDCKEQRCPSDCHGQGRCVDGQ CICHEGFTGLDCGQHSCPSDCNNLGQCVSGRCICNEGYSGEDCSEVSPPKDLVVTEVTE ETVNLAWDNEMRVTEYLVVYTPTHEGGLEMQFRVPGDQTSTIIQELEPGVEYFIRVFAI LENKKSIPVSARVATYLPAPEGLKFKSIKETSVEVEWDPLDIAFETWEIIFRNMNKEDEG EITKSLRRPETSYRQTGLAPGQEYEISLHIVKNNTRGPGLKRVTTTRLDAPSQIEVKDVT DTTALITWFKPLAEIDGIELTYGIKDVPGDRTTIDLTEDENQYSIGNLKPDTEYEVSLISRR GDMSSNPAKETFTTGLDAPRNLRRVSQTDNSITLEWRNGKAAIDSYRIKYAPISGGDHA EVDVPKSQQATTKTTLTGLRPGTEYGIGVSAVKEDKESNPATINAATELDTPKDLQVSE TAETSLTLLWKTPLAKFDRYRLNYSLPTGQWVGVQLPRNTTSYVLRGLEPGQEYNVLL TAEKGRHKSKPARVKASTEQAPELENLTVTEVGWDGLRLNWTAADQAYEHFIIQVQE ANKVEAARNLTVPGSLRAVDIPGLKAATPYTVSIYGVIQGYRTPVLSAEASTGETPNLG EVVVAEVGWDALKLNWTAPEGAYEYFFIQVQEADTVEAAQNLTVPGGLRSTDLPGLK AATHYTITIRGVTQDFSTTPLSVEVLTEEVPDMGNLTVTEVSWDALRLNWTTPDGTYD QFTIQVQEADQVEEAHNLTVPGSLRSMEIPGLRAGTPYTVTLHGEVRGHSTRPLAVEVV TEDLPQLGDLAVSEVGWDGLRLNWTAADNAYEHFVIQVQEVNKVEAAQNLTLPGSLR AVDIPGLEAATPYRVSIYGVIRGYRTPVLSAEASTAKEPEIGNLNVSDITPESFNLSWMA TDGIFETFTIEIIDSNRLLETVEYNISGAERTAHISGLPPSTDFIVYLSGLAPSIRTKTISATA TTEALPLLENLTISDINPYGFTVSWMASENAFDSFLVTVVDSGKLLDPQEFTLSGTQRKL ELRGLITGIGYEVMVSGFTQGHQTKPLRAEIVTEAEPEVDNLLVSDATPDGFRLSWTAD EGVFDNFVLKIRDTKKQSEPLEITLLAPERTRDLTGLREATEYEIELYGISKGRRSQTVSA IATT corresponding to amino acids 1-1708 of TENA_HUMAN_V1, which also corresponds to amino acids 1-1708 of HUMTEN_PEA_(—)1_P26 (SEQ ID NO: 946), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence GTVNKQERTEKSHDSGVFFSQG (SEQ ID NO: 1103) corresponding to amino acids 1709-1730 of HUMTEN_PEA_(—)1_P26 (SEQ ID NO: 946), wherein said first amino acid sequence and second amino acid sequence are contiguous and in a sequential order.

2. An isolated polypeptide encoding for a tail of HUMTEN_PEA_(—)1_P26 (SEQ ID NO: 946), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence GTVNKQERTEKSHDSGVFFSQG (SEQ ID NO: 1103) in HUMTEN_PEA_(—)1_P26 (SEQ ID NO: 946).

It should be noted that the known protein sequence (TENA_HUMAN) has one or more changes than the sequence given at the end of the application and named as being the amino acid sequence for TENA_HUMAN_V1. These changes were previously known to occur and are listed in the table below.

TABLE 43 Changes to TENA_HUMAN_V1 SNP position(s) on amino acid sequence Type of change 371 conflict 540 conflict

The location of the variant protein was determined according to results from a number of different software programs and analyses, including analyses from SignalP and other specialized programs. The variant protein is believed to be located as follows with regard to the cell: secreted. The protein localization is believed to be secreted because both signal-peptide prediction programs predict that this protein has a signal peptide, and neither trans-membrane region prediction program predicts that this protein has a trans-membrane region.

Variant protein HUMTEN_PEA_(—)1_P26 (SEQ ID NO: 946) also has the following non-silent SNPs (Single Nucleotide Polymorphisms) as listed in Table 44, (given according to their position(s) on the amino acid sequence, with the alternative amino acid(s) listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein HUMTEN_PEA_(—)1_P26 (SEQ ID NO: 946) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 44 Amino acid mutations SNP position(s) on amino acid sequence Alternative amino acid(s) Previously known SNP? 149 Q -> * No 213 G -> S Yes 370 V -> L Yes 539 R -> Q Yes 605 V -> I Yes 680 Q -> R Yes 842 V -> L No 850 D -> H Yes 851 L -> V Yes 1066 R -> H No 1677 L -> I Yes

Variant protein HUMTEN_PEA_(—)1_P26 (SEQ ID NO: 946) is encoded by the following transcript(s): HUMTEN_PEA_(—)1_T32 (SEQ ID NO: 869), for which the sequence(s) is/are given at the end of the application. The coding portion of transcript HUMTEN_PEA_(—)1_T32 (SEQ ID NO: 869) is shown in bold; this coding portion starts at position 348 and ends at position 5537. The transcript also has the following SNPs as listed in Table 45 (given according to their position on the nucleotide sequence, with the alternative nucleic acid listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein HUMTEN_PEA_(—)1_P26 (SEQ ID NO: 946) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 45 Nucleic acid SNPs SNP position on nucleotide sequence Alternative nucleic acid Previously known SNP? 115 T -> G Yes 123 A -> G Yes 315 C -> T Yes 434 C -> T Yes 503 C -> T Yes 542 G -> A Yes 623 A -> G Yes 792 C -> T No 984 G -> A Yes 1043 A -> G Yes 1455 G -> T Yes 1963 G -> A Yes 2156 A -> G Yes 2160 G -> A Yes 2386 A -> G Yes 2396 A -> G Yes 2654 G -> A No 2871 G -> T No 2895 G -> C Yes 2898 C -> G Yes 3005 A -> G No 3512 C -> T Yes 3544 G -> A No 3635 A -> G Yes 4922 G -> A No 5376 T -> A Yes 5673 G -> A Yes 6041 G -> C Yes 6081 C -> T Yes 6101 C -> T Yes 6318 G -> A Yes

Variant protein HUMTEN_PEA_(—)1_P27 (SEQ ID NO: 947) according to the present invention has an amino acid sequence as given at the end of the application; it is encoded by transcript(s) HUMTEN_PEA_(—)1_T35 (SEQ ID NO: 870). An alignment is given to the known protein (Tenascin precursor) at the end of the application. One or more alignments to one or more previously published protein sequences are given at the end of the application. A brief description of the relationship of the variant protein according to the present invention to each such aligned protein is as follows:

Comparison report between HUMTEN_PEA_(—)1_P27 (SEQ ID NO: 947) and TENA_HUMAN_V1:

1. An isolated chimeric polypeptide encoding for HUMTEN_PEA_(—)1_P27 (SEQ ID NO: 947), comprising a first amino acid sequence being at least 90% homologous to MGAMTQLLAGVFLAFLALATEGGVLKKVIRHKRQSGVNATLPEENQPVVFNHVYNIK LPVGSQCSVDLESASGEKDLAPPSEPSESFQEHTVDGENQIVFTHRNIPRRACGCAAAP DVKELLSRLEELENLVSSLREQCTAGAGCCLQPATGRLDTRPFCSGRGNFSTEGCGCVC EPGWKGPNCSEPECPGNCHLRGRCIDGQCICDDGFTGEDCSQLACPSDCNDQGKCVNG VCICFEGYAGADCSREICPVPCSEEHGTCVDGLCVCHDGFAGDDCNKPLCLNNCYNRG RCVENECVCDEGFTGEDCSELICPNDCFDRGRCINGTCYCEEGFTGEDCGKPTCPHACH TQGRCEEGQCVCDEGFAGVDCSEKRCPADCHNRGRCVDGRCECDDGFTGADCGELKC PNGCSGHGRCVNGQCVCDEGYTGEDCSQLRCPNDCHSRGRCVEGKCVCEQGFKGYDC SDMSCPNDCHQHGRCVNGMCVCDDGYTGEDCRDRQCPRDCSNRGLCVDGQCVCEDG FTGPDCAELSCPNDCHGRGRCVNGQCVCHEGFMGKDCKEQRCPSDCHGQGRCVDGQ CICHEGFTGLDCGQHSCPSDCNNLGQCVSGRCICNEGYSGEDCSEVSPPKDLVVTEVTE ETVNLAWDNEMRVTEYLVVYTPTHEGGLEMQFRVPGDQTSTIIQELEPGVEYFIRVFAI LENKKSIPVSARVATYLPAPEGLKFKSIKETSVEVEWDPLDIAFETWEIIFRNMNKEDEG EITKSLRRPETSYRQTGLAPGQEYEISLHIVKNNTRGPGLKRVTTTRLDAPSQIEVKDVT DTTALITWFKPLAEIDGIELTYGIKDVPGDRTTIDLTEDENQYSIGNLKPDTEYEVSLISRR GDMSSNPAKETFTTGLDAPRNLRRVSQTDNSITLEWRNGKAAIDSYRIKYAPISGGDHA EVDVPKSQQATTKTTLTGLRPGTEYGIGVSAVKEDKESNPATINAATELDTPKDLQVSE TAETSLTLLWKTPLAKFDRYRLNYSLPTGQWVGVQLPRNTTSYVLRGLEPGQEYNVLL TAEKGRHKSKPARVKASTEQAPELENLTVTEVGWDGLRLNWTAADQAYEHFIIQVQE ANKVEAARNLTVPGSLRAVDIPGLKAATPYTVSIYGVIQGYRTPVLSAEASTGETPNLG EVVVAEVGWDALKLNWTAPEGAYEYFFIQVQEADTVEAAQNLTVPGGLRSTDLPGLK AATHYTITIRGVTQDFSTTPLSVEVLTEEVPDMGNLTVTEVSWDALRLNWTTPDGTYD QFTIQVQEADQVEEAHNLTVPGSLRSMEIPGLRAGTPYTVTLHGEVRGHSTRPLAVEVV T corresponding to amino acids 1-1344 of TENA_HUMAN_V1, which also corresponds to amino acids 1-1344 of HUMTEN_PEA_(—)1_P27 (SEQ ID NO: 947), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence GI corresponding to amino acids 1345-1346 of HUMTEN_PEA_(—)1_P27 (SEQ ID NO: 947), wherein said first amino acid sequence and second amino acid sequence are contiguous and in a sequential order.

It should be noted that the known protein sequence (TENA_HUMAN) has one or more changes than the sequence given at the end of the application and named as being the amino acid sequence for TENA_HUMAN_V1. These changes were previously known to occur and are listed in the table below.

TABLE 46 Changes to TENA_HUMAN_V1 SNP position(s) on amino acid sequence Type of change 371 conflict 540 conflict

The location of the variant protein was determined according to results from a number of different software programs and analyses, including analyses from SignalP and other specialized programs. The variant protein is believed to be located as follows with regard to the cell: secreted. The protein localization is believed to be secreted because both signal-peptide prediction programs predict that this protein has a signal peptide, and neither trans-membrane region prediction program predicts that this protein has a trans-membrane region.

Variant protein HUMTEN_PEA_(—)1_P27 (SEQ ID NO: 947) also has the following non-silent SNPs (Single Nucleotide Polymorphisms) as listed in Table 47, (given according to their position(s) on the amino acid sequence, with the alternative amino acid(s) listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein HUMTEN_PEA_(—)1_P27 (SEQ ID NO: 947) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 47 Amino acid mutations SNP position(s) on amino acid Alternative sequence amino acid(s) Previously known SNP? 149 Q -> * No 213 G -> S Yes 370 V -> L Yes 539 R -> Q Yes 605 V -> I Yes 680 Q -> R Yes 842 V -> L No 850 D -> H Yes 851 L -> V Yes 1066 R -> H No

Variant protein HUMTEN_PEA_(—)1_P27 (SEQ ID NO: 947) is encoded by the following transcript(s): HUMTEN_PEA_(—)1_T35 (SEQ ID NO: 870), for which the sequence(s) is/are given at the end of the application. The coding portion of transcript HUMTEN_PEA_(—)1_T35 (SEQ ID NO: 870) is shown in bold; this coding portion starts at position 348 and ends at position 4385. The transcript also has the following SNPs as listed in Table 48 (given according to their position on the nucleotide sequence, with the alternative nucleic acid listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein HUMTEN_PEA_(—)1_P27 (SEQ ID NO: 947) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 48 Nucleic acid SNPs SNP position on nucleotide Alternative sequence nucleic acid Previously known SNP? 115 T -> G Yes 123 A -> G Yes 315 C -> T Yes 434 C -> T Yes 503 C -> T Yes 542 G -> A Yes 623 A -> G Yes 792 C -> T No 984 G -> A Yes 1043 A -> G Yes 1455 G -> T Yes 1963 G -> A Yes 2156 A -> G Yes 2160 G -> A Yes 2386 A -> G Yes 2396 A -> G Yes 2654 G -> A No 2871 G -> T No 2895 G -> C Yes 2898 C -> G Yes 3005 A -> G No 3512 C -> T Yes 3544 G -> A No 3635 A -> G Yes

Variant protein HUMTEN_PEA_(—)1_P28 (SEQ ID NO: 948) according to the present invention has an amino acid sequence as given at the end of the application; it is encoded by transcript(s) HUMTEN_PEA_(—)1_T36 (SEQ ID NO: 871). An alignment is given to the known protein (Tenascin precursor) at the end of the application. One or more alignments to one or more previously published protein sequences are given at the end of the application. A brief description of the relationship of the variant protein according to the present invention to each such aligned protein is as follows:

Comparison report between HUMTEN_PEA_(—)1_P28 (SEQ ID NO: 948) and TENA_HUMAN_V1:

1. An isolated chimeric polypeptide encoding for HUMTEN_PEA_(—)1_P28 (SEQ ID NO: 948), comprising a first amino acid sequence being at least 90% homologous to MGAMTQLLAGVFLAFLALATEGGVLKKVIRHKRQSGVNATLPEENQPVVFNHVYNIK LPVGSQCSVDLESASGEKDLAPPSEPSESFQEHTVDGENQIVFTHRINIPRRACGCAAAP DVKELLSRLEELENLVSSLREQCTAGAGCCLQPATGRLDTRPFCSGRGNFSTEGCGCVC EPGWKGPNCSEPECPGNCHLRGRCIDGQCICDDGFTGEDCSQLACPSDCNDQGKCVNG VCICFEGYAGADCSREICPVPCSEEHGTCVDGLCVCHDGFAGDDCNKPLCLNNCYNRG RCVENECVCDEGFTGEDCSELICPNDCFDRGRCINGTCYCEEGFTGEDCGKPTCPHACH TQGRCEEGQCVCDEGFAGVDCSEKRCPADCHNRGRCVDGRCECDDGFTGADCGELKC PNGCSGHGRCVNGQCVCDEGYTGEDCSQLRCPNDCHSRGRCVEGKCVCEQGFKGYDC SDMSCPNDCHQHGRCVNGMCVCDDGYTGEDCRDRQCPRDCSNRGLCVDGQCVCEDG FTGPDCAELSCPNDCHGRGRCVNGQCVCHEGFMGKDCKEQRCPSDCHGQGRCVDGQ CICHEGFTGLDCGQHSCPSDCNNLGQCVSGRCICNEGYSGEDCSEVSPPKDLVVTEVTE ETVNLAWDNEMRVTEYLVVYTPTHEGGLEMQFRVPGDQTSTIIQELEPGVEYFIRVFAI LENKKSIPVSARVATYLPAPEGLKFKSIKETSVEVEWDPLDIAFETWEIIFRNMNKEDEG EITKSLRRPETSYRQTGLAPGQEYEISLHIVKNNTRGPGLKRVTTTRLDAPSQIEVKDVT DTTALITWFKPLAEIDGIELTYGIKDVPGDRTTIDLTEDENQYSIGNLKPDTEYEVSLISRR GDMSSNPAKETFTTGLDAPRNLRRVSQTDNSITLEWRNGKAAIDSYRIKYAPISGGDHA EVDVPKSQQATTKTTLTGLRPGTEYGIGVSAVKEDKESNPATINAATELDTPKDLQVSE TAETSLTLLWKTPLAKFDRYRLNYSLPTGQWVGVQLPRNTTSYVLRGLEPGQEYNVLL TAEKGRHKSKPARVKASTEQAPELENLTVTEVGWDGLRLNWTAADQAYEHFIIQVQE ANKVEAARNLTVPGSLRAVDIPGLKAATPYTVSIYGVIQGYRTPVLSAEASTGETPNLG EVVVAEVGWDALKLNWTAPEGAYEYFFIQVQEADTVEAAQNLTVPGGLRSTDLPGLK AATHYTITIRGVTQDFSTTPLSVEVLT corresponding to amino acids 1-1253 of TENA_HUMAN_V1, which also corresponds to amino acids 1-1253 of HUMTEN_PEA_(—)1_P28 (SEQ ID NO: 948), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence GILDEFTNSLPPLCLCSGGIKALSCFKLGSAPTTLGKYQ (SEQ ID NO: 1104) corresponding to amino acids 1254-1292 of HUMTEN_PEA_(—)1_P28 (SEQ ID NO: 948), wherein said first amino acid sequence and second amino acid sequence are contiguous and in a sequential order.

2. An isolated polypeptide encoding for a tail of HUMTEN_PEA_(—)1_P28 (SEQ ID NO: 948), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence GILDEFTNSLPPLCLCSGGIKALSCFKLGSAPTTLGKYQ (SEQ ID NO: 1104) in HUMTEN_PEA_(—)1_P28 (SEQ ID NO: 948).

It should be noted that the known protein sequence (TENA_HUMAN) has one or more changes than the sequence given at the end of the application and named as being the amino acid sequence for TENA_HUMAN_V1. These changes were previously known to occur and are listed in the table below.

TABLE 49 Changes to TENA_HUMAN_V1 SNP position(s) on amino acid sequence Type of change 371 conflict 540 conflict

The location of the variant protein was determined according to results from a number of different software programs and analyses, including analyses from SignalP and other specialized programs. The variant protein is believed to be located as follows with regard to the cell: secreted. The protein localization is believed to be secreted because both signal-peptide prediction programs predict that this protein has a signal peptide, and neither trans-membrane region prediction program predicts that this protein has a trans-membrane region.

Variant protein HUMTEN_PEA_(—)1_P28 (SEQ ID NO: 948) also has the following non-silent SNPs (Single Nucleotide Polymorphisms) as listed in Table 50, (given according to their position(s) on the amino acid sequence, with the alternative amino acid(s) listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein HUMTEN_PEA_(—)1_P28 (SEQ ID NO: 948) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 50 Amino acid mutations SNP position(s) on amino acid Alternative amino Previously known sequence acid(s) SNP? 149 Q -> * No 213 G -> S Yes 370 V -> L Yes 539 R -> Q Yes 605 V -> I Yes 680 Q -> R Yes 842 V -> L No 850 D -> H Yes 851 L -> V Yes 1066 R -> H No

Variant protein HUMTEN_PEA_(—)1_P28 (SEQ ID NO: 948) is encoded by the following transcript(s): HUMTEN_PEA_(—)1_T36 (SEQ ID NO: 871), for which the sequence(s) is/are given at the end of the application. The coding portion of transcript HUMTEN_PEA_(—)1_T36 (SEQ ID NO: 871) is shown in bold; this coding portion starts at position 348 and ends at position 4223. The transcript also has the following SNPs as listed in Table 51 (given according to their position on the nucleotide sequence, with the alternative nucleic acid listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein HUMTEN_PEA_(—)1_P28 (SEQ ID NO: 948) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 51 Nucleic acid SNPs SNP position on nucleotide Alternative sequence nucleic acid Previously known SNP? 115 T -> G Yes 123 A -> G Yes 315 C -> T Yes 434 C -> T Yes 503 C -> T Yes 542 G -> A Yes 623 A -> G Yes 792 C -> T No 984 G -> A Yes 1043 A -> G Yes 1455 G -> T Yes 1963 G -> A Yes 2156 A -> G Yes 2160 G -> A Yes 2386 A -> G Yes 2396 A -> G Yes 2654 G -> A No 2871 G -> T No 2895 G -> C Yes 2898 C -> G Yes 3005 A -> G No 3512 C -> T Yes 3544 G -> A No 3635 A -> G Yes

Variant protein HUMTEN_PEA_(—)1_P29 (SEQ ID NO: 949) according to the present invention has an amino acid sequence as given at the end of the application; it is encoded by transcript(s) HUMTEN_PEA_(—)1_T37 (SEQ ID NO: 872). An alignment is given to the known protein (Tenascin precursor) at the end of the application. One or more alignments to one or more previously published protein sequences are given at the end of the application. A brief description of the relationship of the variant protein according to the present invention to each such aligned protein is as follows:

Comparison report between HUMTEN_PEA_(—)1_P29 (SEQ ID NO: 949) and TENA_HUMAN_V1:

1. An isolated chimeric polypeptide encoding for HUMTEN_PEA_(—)1_P29 (SEQ ID NO: 949), comprising a first amino acid sequence being at least 90% homologous to MGAMTQLLAGVFLAFLALATEGGVLKKVIRHKRQSGVNATLPEENQPVVFNHVYNIK LPVGSQCSVDLESASGEKDLAPPSEPSESFQEHTVDGENQIVFTHRINIPRRACGCAAAP DVKELLSRLEELENLVSSLREQCTAGAGCCLQPATGRLDTRPFCSGRGNFSTEGCGCVC EPGWKGPNCSEPECPGNCHLRGRCIDGQCICDDGFTGEDCSQLACPSDCNDQGKCVNG VCICFEGYAGADCSREICPVPCSEEHGTCVDGLCVCHDGFAGDDCNKPLCLNNCYNRG RCVENECVCDEGFTGEDCSELICPNDCFDRGRCINGTCYCEEGFTGEDCGKPTCPHACH TQGRCEEGQCVCDEGFAGVDCSEKRCPADCHNRGRCVDGRCECDDGFTGADCGELKC PNGCSGHGRCVNGQCVCDEGYTGEDCSQLRCPNDCHSRGRCVEGKCVCEQGFKGYDC SDMSCPNDCHQHGRCVNGMCVCDDGYTGEDCRDRQCPRDCSNRGLCVDGQCVCEDG FTGPDCAELSCPNDCHGRGRCVNGQCVCHEGFMGKDCKEQRCPSDCHGQGRCVDGQ CICHEGFTGLDCGQHSCPSDCNNLGQCVSGRCICNEGYSGEDCSEVSPPKDLVVTEVTE ETVNLAWDNEMRVTEYLVVYTPTHEGGLEMQFRVPGDQTSTIIQELEPGVEYFIRVFAI LENKKSIPVSARVATYLPAPEGLKFKSIKETSVEVEWDPLDIAFETWEIIFRNMNKEDEG EITKSLRRPETSYRQTGLAPGQEYEISLHIVKNNTRGPGLKRVTTTRLDAPSQIEVKDVT DTTALITWFKPLAEIDGIELTYGIKDVPGDRTTIDLTEDENQYSIGNLKPDTEYEVSLISRR GDMSSNPAKETFTTGLDAPRNLRRVSQTDNSITLEWRNGKAAIDSYRIKYAPISGQDHA EVDVPKSQQATTKTTLTGLRPGTEYGIGVSAVKEDKESNPATINAATELDTPKDLQVSE TAETSLTLLWKTPLAKFDRYRLNYSLPTGQWVGVQLPRNTTSYVLRGLEPGQEYNVLL TAEKGRHKSKPARVKAST corresponding to amino acids 1-1071 of TENA_HUMAN_V1, which also corresponds to amino acids 1-1071 of HUMTEN_PEA_(—)1_P29 (SEQ ID NO: 949), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence GESALSFLQTLG (SEQ ID NO: 1105) corresponding to amino acids 1072-1083 of HUMTEN_PEA_(—)1_P29 (SEQ ID NO: 949), wherein said first amino acid sequence and second amino acid sequence are contiguous and in a sequential order.

2. An isolated polypeptide encoding for a tail of HUMTEN_PEA_(—)1_P29 (SEQ ID NO: 949), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence GESALSFLQTLG (SEQ ID NO: 1105) in HUMTEN_PEA_(—)1_P29 (SEQ ID NO: 949).

It should be noted that the known protein sequence (TENA_HUMAN) has one or more changes than the sequence given at the end of the application and named as being the amino acid sequence for TENA_HUMAN_V1. These changes were previously known to occur and are listed in the table below.

TABLE 52 Changes to TENA_HUMAN_V1 SNP position(s) on amino acid sequence Type of change 371 conflict 540 conflict

The location of the variant protein was determined according to results from a number of different software programs and analyses, including analyses from SignalP and other specialized programs. The variant protein is believed to be located as follows with regard to the cell: secreted. The protein localization is believed to be secreted because both signal-peptide prediction programs predict that this protein has a signal peptide, and neither trans-membrane region prediction program predicts that this protein has a trans-membrane region.

Variant protein HUMTEN_PEA_(—)1_P29 (SEQ ID NO: 949) also has the following non-silent SNPs (Single Nucleotide Polymorphisms) as listed in Table 53, (given according to their position(s) on the amino acid sequence, with the alternative amino acid(s) listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein HUMTEN_PEA_(—)1_P29 (SEQ ID NO: 949) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 53 Amino acid mutations SNP position(s) on amino acid Alternative sequence amino acid(s) Previously known SNP? 149 Q -> * No 213 G -> S Yes 370 V -> L Yes 539 R -> Q Yes 605 V -> I Yes 680 Q -> R Yes 842 V -> L No 850 D -> H Yes 851 L -> V Yes 1066 R -> H No

Variant protein HUMTEN_PEA_(—)1_P29 (SEQ ID NO: 949) is encoded by the following transcript(s): HUMTEN_PEA_(—)1_T37 (SEQ ID NO: 872), for which the sequence(s) is/are given at the end of the application. The coding portion of transcript HUMTEN_PEA_(—)1_T37 (SEQ ID NO: 872) is shown in bold; this coding portion starts at position 348 and ends at position 3596. The transcript also has the following SNPs as listed in Table 54 (given according to their position on the nucleotide sequence, with the alternative nucleic acid listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein HUMTEN_PEA_(—)1_P29 (SEQ ID NO: 949) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 54 Nucleic acid SNPs SNP position on nucleotide Alternative sequence nucleic acid Previously known SNP? 115 T -> G Yes 123 A -> G Yes 315 C -> T Yes 434 C -> T Yes 503 C -> T Yes 542 G -> A Yes 623 A -> G Yes 792 C -> T No 984 G -> A Yes 1043 A -> G Yes 1455 G -> T Yes 1963 G -> A Yes 2156 A -> G Yes 2160 G -> A Yes 2386 A -> G Yes 2396 A -> G Yes 2654 G -> A No 2871 G -> T No 2895 G -> C Yes 2898 C -> G Yes 3005 A -> G No 3512 C -> T Yes 3544 G -> A No 3693 A -> C Yes

Variant protein HUMTEN_PEA_(—)1_P30 (SEQ ID NO: 950) according to the present invention has an amino acid sequence as given at the end of the application; it is encoded by transcript(s) HUMTEN_PEA_(—)1_T39 (SEQ ID NO: 873). An alignment is given to the known protein (Tenascin precursor) at the end of the application. One or more alignments to one or more previously published protein sequences are given at the end of the application. A brief description of the relationship of the variant protein according to the present invention to each such aligned protein is as follows:

Comparison report between HUMTEN_PEA_(—)1_P30 (SEQ ID NO: 950) and TENA_HUMAN_V1:

1. An isolated chimeric polypeptide encoding for HUMTEN_PEA_(—)1_P30 (SEQ ID NO: 950), comprising a first amino acid sequence being at least 90% homologous to MGAMTQLLAGVFLAFLALATEGGVLKKVIRHKRQSGVNATLPEENQPVVFNHVYNIK LPVGSQCSVDLESASGEKDLAPPSEPSESFQEHTVDGENQIVFTHRINIPRRACGCAAAP DVKELLSRLEELENLVSSLREQCTAGAGCCLQPATGRLDTRPFCSGRGNFSTEGCGCVC EPGWKGPNCSEPECPGNCHLRGRCIDGQCICDDGFTGEDCSQLACPSDCNDQGKCVNG VCICFEGYAGADCSREICPVPCSEEHGTCVDGLCVCHDGFAGDDCNKPLCLNNCYNRG RCVENECVCDEGFTGEDCSELICPNDCFDRGRCINGTCYCEEGFTGEDCGKPTCPHACH TQGRCEEGQCVCDEGFAGVDCSEKRCPADCHNRGRCVDGRCECDDGFTGADCGELKC PNGCSGHGRCVNGQCVCDEGYTGEDCSQLRCPNDCHSRGRCVEGKCVCEQGFKGYDC SDMSCPNDCHQHGRCVNGMCVCDDGYTGEDCRDRQCPRDCSNRGLCVDGQCVCEDG FTGPDCAELSCPNDCHGRGRCVNGQCVCHEGFMGKDCKEQRCPSDCHGQGRCVDGQ CICHEGFTGLDCGQHSCPSDCNNLGQCVSGRCICNEGYSGEDCSEVSPPKDLVVTEVTE ETVNLAWDNEMRVTEYLVVYTPTHEGGLEMQFRVPGDQTSTIIQELEPGVEYFIRVFAI LENKKSIPVSARVATYLPAPEGLKFKSIKETSVEVEWDPLDIAFETWEIIFRNMNKEDEG EITKSLRRPETSYRQTGLAPGQEYEISLHIVKNNTRGPGLKRVTTTRLDAPSQIEVKDVT DTTALITWFKPLAEIDGIELTYGIKDVPGDRTTIDLTEDENQYSIGNLKPDTEYEVSLISRR GDMSSNPAKETFTTGLDAPRNLRRVSQTDNSITLEWRNGKAAIDSYRIKYAPISGGDHA EVDVPKSQQATTKTTLTG corresponding to amino acids 1-954 of TENA_HUMAN_V1, which also corresponds to amino acids 1-954 of HUMTEN_PEA_(—)1_P30 (SEQ ID NO: 950), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence ELCISASLSQPALEGP (SEQ ID NO: 1106) corresponding to amino acids 955-970 of HUMTEN_PEA_(—)1_P30 (SEQ ID NO: 950), wherein said first amino acid sequence and second amino acid sequence are contiguous and in a sequential order.

2. An isolated polypeptide encoding for a tail of HUMTEN_PEA_(—)1_P30 (SEQ ID NO: 950), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence ELCISASLSQPALEGP (SEQ ID NO: 1106) in HUMTEN_PEA_(—)1_P30 (SEQ ID NO: 950).

It should be noted that the known protein sequence (TENA_HUMAN) has one or more changes than the sequence given at the end of the application and named as being the amino acid sequence for TENA_HUMAN_V1. These changes were previously known to occur and are listed in the table below.

TABLE 55 Changes to TENA_HUMAN_V1 SNP position(s) on amino acid sequence Type of change 371 conflict 540 conflict

The location of the variant protein was determined according to results from a number of different software programs and analyses, including analyses from SignalP and other specialized programs. The variant protein is believed to be located as follows with regard to the cell: secreted. The protein localization is believed to be secreted because of manual inspection of known protein localization and/or gene structure.

Variant protein HUMTEN_PEA_(—)1_P30 (SEQ ID NO: 950) also has the following non-silent SNPs (Single Nucleotide Polymorphisms) as listed in Table 56, (given according to their position(s) on the amino acid sequence, with the alternative amino acid(s) listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein HUMTEN_PEA_(—)1_P30 (SEQ ID NO: 950) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 56 Amino acid mutations SNP position(s) on amino acid Alternative sequence amino acid(s) Previously known SNP? 149 Q -> * No 213 G -> S Yes 370 V -> L Yes 539 R -> Q Yes 605 V -> I Yes 680 Q -> R Yes 842 V -> L No 850 D -> H Yes 851 L -> V Yes 969 G -> R Yes

Variant protein HUMTEN_PEA_(—)1_P30 (SEQ ID NO: 950) is encoded by the following transcript(s): HUMTEN_PEA_(—)1_T39 (SEQ ID NO: 873), for which the sequence(s) is/are given at the end of the application. The coding portion of transcript HUMTEN_PEA_(—)1_T39 (SEQ ID NO: 873) is shown in bold; this coding portion starts at position 348 and ends at position 3257. The transcript also has the following SNPs as listed in Table 57 (given according to their position on the nucleotide sequence, with the alternative nucleic acid listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein HUMTEN_PEA_(—)1_P30 (SEQ ID NO: 950) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 57 Nucleic acid SNPs SNP position on nucleotide Alternative sequence nucleic acid Previously known SNP? 115 T -> G Yes 123 A -> G Yes 315 C -> T Yes 434 C -> T Yes 503 C -> T Yes 542 G -> A Yes 623 A -> G Yes 792 C -> T No 984 G -> A Yes 1043 A -> G Yes 1455 G -> T Yes 1963 G -> A Yes 2156 A -> G Yes 2160 G -> A Yes 2386 A -> G Yes 2396 A -> G Yes 2654 G -> A No 2871 G -> T No 2895 G -> C Yes 2898 C -> G Yes 3005 A -> G No 3252 G -> C Yes

Variant protein HUMTEN_PEA_(—)1_P31 (SEQ ID NO: 951) according to the present invention has an amino acid sequence as given at the end of the application; it is encoded by transcript(s) HUMTEN_PEA_(—)1_T40 (SEQ ID NO: 874). An alignment is given to the known protein (Tenascin precursor) at the end of the application. One or more alignments to one or more previously published protein sequences are given at the end of the application. A brief description of the relationship of the variant protein according to the present invention to each such aligned protein is as follows:

Comparison report between HUMTEN_PEA_(—)1_P31 (SEQ ID NO: 951) and TENA_HUMAN_V1:

1. An isolated chimeric polypeptide encoding for HUMTEN_PEA_(—)1_P31 (SEQ ID NO: 951), comprising a first amino acid sequence being at least 90% homologous to MGAMTQLLAGVFLAFLALATEGGVLKKVIRHKRQSGVNATLPEENQPVVFNHVYNIK LPVGSQCSVDLESASGEKDLAPPSEPSESFQEHTVDGENQIVFTHRINIPRRACGCAAAP DVKELLSRLEELENLVSSLREQCTAGAGCCLQPATGRLDTRPFCSGRGNFSTEGCGCVC EPGWKGPNCSEPECPGNCHLRGRCIDGQCICDDGFTGEDCSQLACPSDCNDQGKCVNG VCICFEGYAGADCSREICPVPCSEEHGTCVDGLCVCHDGFAGDDCNKPLCLNNCYNRG RCVENECVCDEGFTGEDCSELICPNDCFDRGRCINGTCYCEEGFTGEDCGKPTCPHACH TQGRCEEGQCVCDEGFAGVDCSEKRCPADCHNRGRCVDGRCECDDGFTGADCGELKC PNGCSGHGRCVNGQCVCDEGYTGEDCSQLRCPNDCHSRGRCVEGKCVCEQGFKGYDC SDMSCPNDCHQHGRCVNGMCVCDDGYTGEDCRDRQCPRDCSNRGLCVDGQCVCEDG FTGPDCAELSCPNDCHGRGRCVNGQCVCHEGFMGKDCKEQRCPSDCHGQGRCVDGQ CICHEGFTGLDCGQHSCPSDCNNLGQCVSGRCICNEGYSGEDCSEVSPPKDLVVTEVTE ETVNLAWDNEMRVTEYLVVYTPTHEGGLEMQFRVPGDQTSTIIQELEPGVEYFIRVFAI LENKKSIPVSARVATYLPAPEGLKFKSIKETSVEVEWDPLDIAFETWEIIFRNMNKEDEG EITKSLRRPETSYRQTGLAPGQEYEISLHIVKNNTRGPGLKRVTTTR corresponding to amino acids 1-802 of TENA_HUMAN_V1, which also corresponds to amino acids 1-802 of HUMTEN_PEA_(—)1_P31 (SEQ ID NO: 951), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence EYHL (SEQ ID NO: 1107) corresponding to amino acids 803-806 of HUMTEN_PEA_(—)1_P31 (SEQ ID NO: 951), wherein said first amino acid sequence and second amino acid sequence are contiguous and in a sequential order.

2. An isolated polypeptide encoding for a tail of HUMTEN_PEA_(—)1_P31 (SEQ ID NO: 951), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence EYHL (SEQ ID NO: 1107) in HUMTEN_PEA_(—)1_P31 (SEQ ID NO: 951).

It should be noted that the known protein sequence (TENA_HUMAN) has one or more changes than the sequence given at the end of the application and named as being the amino acid sequence for TENA_HUMAN_V1. These changes were previously known to occur and are listed in the table below.

TABLE 58 Changes to TENA_HUMAN_V1 SNP position(s) on amino acid sequence Type of change 371 conflict 540 conflict

The location of the variant protein was determined according to results from a number of different software programs and analyses, including analyses from SignalP and other specialized programs. The variant protein is believed to be located as follows with regard to the cell: secreted. The protein localization is believed to be secreted because both signal-peptide prediction programs predict that this protein has a signal peptide, and neither trans-membrane region prediction program predicts that this protein has a trans-membrane region.

Variant protein HUMTEN_PEA_(—)1_P31 (SEQ ID NO: 951) also has the following non-silent SNPs (Single Nucleotide Polymorphisms) as listed in Table 59, (given according to their position(s) on the amino acid sequence, with the alternative amino acid(s) listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein HUMTEN_PEA_(—)1_P31 (SEQ ID NO: 951) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 59 Amino acid mutations SNP position(s) on amino acid Alternative sequence amino acid(s) Previously known SNP? 149 Q -> * No 213 G -> S Yes 370 V -> L Yes 539 R -> Q Yes 605 V -> I Yes 680 Q -> R Yes

Variant protein HUMTEN_PEA_(—)1_P31 (SEQ ID NO: 951) is encoded by the following transcript(s): HUMTEN_PEA_(—)1_T40 (SEQ ID NO: 874), for which the sequence(s) is/are given at the end of the application. The coding portion of transcript HUMTEN_PEA_(—)1_T40 (SEQ ID NO: 874) is shown in bold; this coding portion starts at position 348 and ends at position 2765. The transcript also has the following SNPs as listed in Table 60 (given according to their position on the nucleotide sequence, with the alternative nucleic acid listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein HUMTEN_PEA_(—)1_P31 (SEQ ID NO: 951) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 60 Nucleic acid SNPs SNP position on nucleotide Alternative sequence nucleic acid Previously known SNP? 115 T -> G Yes 123 A -> G Yes 315 C -> T Yes 434 C -> T Yes 503 C -> T Yes 542 G -> A Yes 623 A -> G Yes 792 C -> T No 984 G -> A Yes 1043 A -> G Yes 1455 G -> T Yes 1963 G -> A Yes 2156 A -> G Yes 2160 G -> A Yes 2386 A -> G Yes 2396 A -> G Yes 2654 G -> A No

Variant protein HUMTEN_PEA_(—)1_P32 (SEQ ID NO: 952) according to the present invention has an amino acid sequence as given at the end of the application; it is encoded by transcript(s) HUMTEN_PEA_(—)1_T41 (SEQ ID NO: 875). An alignment is given to the known protein (Tenascin precursor) at the end of the application. One or more alignments to one or more previously published protein sequences are given at the end of the application. A brief description of the relationship of the variant protein according to the present invention to each such aligned protein is as follows:

Comparison report between HUMTEN_PEA_(—)1_P32 (SEQ ID NO: 952) and TENA_HUMAN_V1:

1. An isolated chimeric polypeptide encoding for HUMTEN_PEA_(—)1_P32 (SEQ ID NO: 952), comprising a first amino acid sequence being at least 90% homologous to MGAMTQLLAGVFLAFLALATEGGVLKKVIRHKRQSGVNATLPEENQPVVFNHVYNIK LPVGSQCSVDLESASGEKDLAPPSEPSESFQEHTVDGENQIVFTHRINIPRRACGCAAAP DVKELLSRLEELENLVSSLREQCTAGAGCCLQPATGRLDTRPFCSGRGNFSTEGCGCVC EPGWKGPNCSEPECPGNCHLRGRCIDGQCICDDGFTGEDCSQLACPSDCNDQGKCVNG VCICFEGYAGADCSREICPVPCSEEHGTCVDGLCVCHDGFAGDDCNKPLCLNNCYNRG RCVENECVCDEGFTGEDCSELICPNDCFDRGRCINGTCYCEEGFTGEDCGKPTCPHACH TQGRCEEGQCVCDEGFAGVDCSEKRCPADCHNRGRCVDGRCECDDGFTGADCGELKC PNGCSGHGRCVNGQCVCDEGYTGEDCSQLRCPNDCHSRGRCVEGKCVCEQGFKGYDC SDMSCPNDCHQHGRCVNGMCVCDDGYTGEDCRDRQCPRDCSNRGLCVDGQCVCEDG FTGPDCAELSCPNDCHGRGRCVNGQCVCHEGFMGKDCKEQRCPSDCHGQGRCVDGQ CICHEGFTGLDCGQHSCPSDCNNLGQCVSGRCICNEGYSGEDCSEVSPPKDLVVTEVTE ETVNLAWDNEMRVTEYLVVYTPTHEGGLEMQFRVPGDQTSTIIQELEPGVEYFIRVFAI LENKKSIPVSARVAT corresponding to amino acids 1-710 of TENA_HUMAN_V1, which also corresponds to amino acids 1-710 of HUMTEN_PEA_(—)1_P32 (SEQ ID NO: 952), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence CE corresponding to amino acids 711-712 of HUMTEN_PEA_(—)1_P32 (SEQ ID NO: 952), wherein said first amino acid sequence and second amino acid sequence are contiguous and in a sequential order.

It should be noted that the known protein sequence (TENA_HUMAN) has one or more changes than the sequence given at the end of the application and named as being the amino acid sequence for TENA_HUMAN_V1. These changes were previously known to occur and are listed in the table below.

TABLE 61 Changes to TENA_HUMAN_V1 SNP position(s) on amino acid sequence Type of change 371 conflict 540 conflict

The location of the variant protein was determined according to results from a number of different software programs and analyses, including analyses from SignalP and other specialized programs. The variant protein is believed to be located as follows with regard to the cell: secreted. The protein localization is believed to be secreted because both signal-peptide prediction programs predict that this protein has a signal peptide, and neither trans-membrane region prediction program predicts that this protein has a trans-membrane region.

Variant protein HUMTEN_PEA_(—)1_P32 (SEQ ID NO: 952) also has the following non-silent SNPs (Single Nucleotide Polymorphisms) as listed in Table 62, (given according to their position(s) on the amino acid sequence, with the alternative amino acid(s) listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein HUMTEN_PEA_(—)1_P32 (SEQ ID NO: 952) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 62 Amino acid mutations SNP position(s) on amino acid Alternative sequence amino acid(s) Previously known SNP? 149 Q -> * No 213 G -> S Yes 370 V -> L Yes 539 R -> Q Yes 605 V -> I Yes 680 Q -> R Yes

Variant protein HUMTEN_PEA_(—)1_P32 (SEQ ID NO: 952) is encoded by the following transcript(s): HUMTEN_PEA_(—)1_T41 (SEQ ID NO: 875), for which the sequence(s) is/are given at the end of the application. The coding portion of transcript HUMTEN_PEA_(—)1_T41 (SEQ ID NO: 875) is shown in bold; this coding portion starts at position 348 and ends at position 2483. The transcript also has the following SNPs as listed in Table 63 (given according to their position on the nucleotide sequence, with the alternative nucleic acid listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein HUMTEN_PEA_(—)1_P32 (SEQ ID NO: 952) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 63 Nucleic acid SNPs SNP position on nucleotide Alternative sequence nucleic acid Previously known SNP? 115 T -> G Yes 123 A -> G Yes 315 C -> T Yes 434 C -> T Yes 503 C -> T Yes 542 G -> A Yes 623 A -> G Yes 792 C -> T No 984 G -> A Yes 1043 A -> G Yes 1455 G -> T Yes 1963 G -> A Yes 2156 A -> G Yes 2160 G -> A Yes 2386 A -> G Yes 2396 A -> G Yes 2516 T -> C Yes 2531 C -> T No 2850 G -> A Yes 2886 G -> A Yes

As noted above, cluster HUMTEN features 57 segment(s), which were listed in Table 2 above and for which the sequence(s) are given at the end of the application. These segment(s) are portions of nucleic acid sequence(s) which are described herein separately because they are of particular interest. A description of each segment according to the present invention is now provided.

Segment cluster HUMTEN_PEA_(—)1_node_(—)0 (SEQ ID NO: 876) according to the present invention is supported by 15 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HUMTEN_PEA_(—)1_T4 (SEQ ID NO: 857), HUMTEN_PEA_(—)1_T5 (SEQ ID NO: 858) HUMTEN_PEA_(—)1_T5 (SEQ ID NO: 858) (SEQ ID NO: 859), HUMTEN_PEA_(—)1_T6 (SEQ ID NO: 859), HUMTEN_PEA_(—)1_T7 (SEQ ID NO: 860), HUMTEN_PEA_(—)1_T11 (SEQ ID NO: 861), HUMTEN_PEA_(—)1_T14 (SEQ ID NO: 862), HUMTEN_PEA_(—)1_T16 (SEQ ID NO: 863), HUMTEN_PEA_(—)1_T17 (SEQ ID NO: 864), HUMTEN_PEA_(—)1_T18 (SEQ ID NO: 865), HUMTEN_PEA_(—)1_T19 (SEQ ID NO: 866), HUMTEN_PEA_(—)1_T20 (SEQ ID NO. 867), HUMTEN_PEA_(—)1_T23 (SEQ ID NO: 868), HUMTEN_PEA_(—)1_T32 (SEQ ID NO: 869), HUMTEN_PEA_(—)1_T35 (SEQ ID NO: 870), HUMTEN_PEA_(—)1_T36 (SEQ ID NO: 871), HUMTEN_PEA_(—)1_T37 (SEQ ID NO: 872), HUMTEN_PEA_(—)1_T39 (SEQ ID NO: 873), HUMTEN_PEA_(—)1_T40 (SEQ ID NO: 874) and HUMTEN_PEA_(—)1_T41 (SEQ ID NO: 875). Table 64 below describes the starting and ending position of this segment on each transcript.

TABLE 64 Segment location on transcripts Segment Segment Transcript name starting position ending position HUMTEN_PEA_1_T4 1 211 (SEQ ID NO: 857) HUMTEN_PEA_1_T5 1 211 (SEQ ID NO: 858) HUMTEN_PEA_1_T5 (SEQ ID NO: 858) (SEQ ID NO: 859) HUMTEN_PEA_1_T6 1 211 (SEQ ID NO: 859) HUMTEN_PEA_1_T7 1 211 (SEQ ID NO: 860) HUMTEN_PEA_1_T11 1 211 (SEQ ID NO: 861) HUMTEN_PEA_1_T14 1 211 (SEQ ID NO: 862) HUMTEN_PEA_1_T16 1 211 (SEQ ID NO: 863) HUMTEN_PEA_1_T17 1 211 (SEQ ID NO: 864) HUMTEN_PEA_1_T18 1 211 (SEQ ID NO: 865) HUMTEN_PEA_1_T19 1 211 (SEQ ID NO: 866) HUMTEN_PEA_I_T20 1 211 (SEQ ID NO. 867) HUMTEN_PEA_1_T23 1 211 (SEQ ID NO: 868) HUMTEN_PEA_1_T32 1 211 (SEQ ID NO: 869) HUMTEN_PEA_1_T35 1 211 (SEQ ID NO: 870) HUMTEN_PEA_1_T36 1 211 (SEQ ID NO: 871) HUMTEN_PEA_1_T37 1 211 (SEQ ID NO: 872) HUMTEN_PEA_1_T39 1 211 (SEQ ID NO: 873) HUMTEN_PEA_1_T40 1 211 (SEQ ID NO: 874) HUMTEN_PEA_1_T41 1 211 (SEQ ID NO: 875)

Segment cluster HUMTEN_PEA_(—)1_node_(—)2 (SEQ ID NO: 877) according to the present invention is supported by 25 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HUMTEN_PEA_(—)1_T4 (SEQ ID NO: 857), HUMTEN_PEA_(—)1_T5 (SEQ ID NO: 858) HUMTEN_PEA_(—)1_T5 (SEQ ID NO: 858) (SEQ ID NO: 859), HUMTEN_PEA_(—)1_T6 (SEQ ID NO: 859), HUMTEN_PEA_(—)1_T7 (SEQ ID NO: 860), HUMTEN_PEA_(—)1_T11 (SEQ ID NO: 861), HUMTEN_PEA_(—)1_T14 (SEQ ID NO: 862), HUMTEN_PEA_(—)1_T16 (SEQ ID NO: 863), HUMTEN_PEA_(—)1_T17 (SEQ ID NO: 864), HUMTEN_PEA_(—)1_T18 (SEQ ID NO: 865), HUMTEN_PEA_(—)1_T19 (SEQ ID NO: 866), HUMTEN_PEA_(—)1_T20 (SEQ ID NO. 867), HUMTEN_PEA_(—)1_T23 (SEQ ID NO: 868), HUMTEN_PEA_(—)1_T32 (SEQ ID NO: 869), HUMTEN_PEA_(—)1_T35 (SEQ ID NO: 870), HUMTEN_PEA_(—)1_T36 (SEQ ID NO: 871), HUMTEN_PEA_(—)1_T37 (SEQ ID NO: 872), HUMTEN_PEA_(—)1_T39 (SEQ ID NO: 873), HUMTEN_PEA_(—)1_T40 (SEQ ID NO: 874) and HUMTEN_PEA_(—)1_T41 (SEQ ID NO: 875). Table 65 below describes the starting and ending position of this segment on each transcript.

TABLE 65 Segment location on transcripts Segment Segment Transcript name starting position ending position HUMTEN_PEA_1_T4 212 804 (SEQ ID NO: 857) HUMTEN_PEA_1_T5 212 804 (SEQ ID NO: 858) HUMTEN_PEA_1_T5 (SEQ ID NO: 858) (SEQ ID NO: 859) HUMTEN_PEA_1_T6 212 804 (SEQ ID NO: 859) HUMTEN_PEA_1_T7 212 804 (SEQ ID NO: 860) HUMTEN_PEA_1_T11 212 804 (SEQ ID NO: 861) HUMTEN_PEA_1_T14 212 804 (SEQ ID NO: 862) HUMTEN_PEA_1_T16 212 804 (SEQ ID NO: 863) HUMTEN_PEA_1_T17 212 804 (SEQ ID NO: 864) HUMTEN_PEA_1_T18 212 804 (SEQ ID NO: 865) HUMTEN_PEA_1_T19 212 804 (SEQ ID NO: 866) HUMTEN_PEA_1_T20 212 804 (SEQ ID NO. 867) HUMTEN_PEA_1_T23 212 804 (SEQ ID NO: 868) HUMTEN_PEA_1_T32 212 804 (SEQ ID NO: 869) HUMTEN_PEA_1_T35 212 804 (SEQ ID NO: 870) HUMTEN_PEA_1_T36 212 804 (SEQ ID NO: 871) HUMTEN_PEA_1_T37 212 804 (SEQ ID NO: 872) HUMTEN_PEA_1_T39 212 804 (SEQ ID NO: 873) HUMTEN_PEA_1_T40 212 804 (SEQ ID NO: 874) HUMTEN_PEA_1_T41 212 804 (SEQ ID NO: 875)

Segment cluster HUMTEN_PEA_(—)1_node_(—)5 (SEQ ID NO: 878) according to the present invention is supported by 34 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HUMTEN_PEA_(—)1_T4 (SEQ ID NO: 857), HUMTEN_PEA_(—)1_T5 (SEQ ID NO: 858) HUMTEN_PEA_(—)1_T5 (SEQ ID NO: 858) (SEQ ID NO: 859), HUMTEN_PEA_(—)1_T6 (SEQ ID NO: 859), HUMTEN_PEA_(—)1_T7 (SEQ ID NO: 860), HUMTEN_PEA_(—)1_T11 (SEQ ID NO: 861), HUMTEN_PEA_(—)1_T14 (SEQ ID NO: 862), HUMTEN_PEA_(—)1_T16 (SEQ ID NO: 863), HUMTEN_PEA_(—)1_T17 (SEQ ID NO: 864), HUMTEN_PEA_(—)1_T18 (SEQ ID NO: 865), HUMTEN_PEA_(—)1_T19 (SEQ ID NO: 866), HUMTEN_PEA_(—)1_T20 (SEQ ID NO. 867), HUMTEN_PEA_(—)1_T23 (SEQ ID NO: 868), HUMTEN_PEA_(—)1_T32 (SEQ ID NO: 869), HUMTEN_PEA_(—)1_T35 (SEQ ID NO: 870), HUMTEN_PEA_(—)1_T36 (SEQ ID NO: 871), HUMTEN_PEA_(—)1_T37 (SEQ ID NO: 872), HUMTEN_PEA_(—)1_T39 (SEQ ID NO: 873), HUMTEN_PEA_(—)1_T40 (SEQ ID NO: 874) and HUMTEN_PEA_(—)1_T41 (SEQ ID NO: 875). Table 66 below describes the starting and ending position of this segment on each transcript.

TABLE 66 Segment location on transcripts Segment Segment Transcript name starting position ending position HUMTEN_PEA_1_T4 805 1672 (SEQ ID NO: 857) HUMTEN_PEA_1_T5 805 1672 (SEQ ID NO: 858) HUMTEN_PEA_1_T5 (SEQ ID NO: 858) (SEQ ID NO: 859) HUMTEN_PEA_1_T6 805 1672 (SEQ ID NO: 859) HUMTEN_PEA_1_T7 805 1672 (SEQ ID NO: 860) HUMTEN_PEA_1_T11 805 1672 (SEQ ID NO: 861) HUMTEN_PEA_1_T14 805 1672 (SEQ ID NO: 862) HUMTEN_PEA_1_T16 805 1672 (SEQ ID NO: 863) HUMTEN_PEA_1_T17 805 1672 (SEQ ID NO: 864) HUMTEN_PEA_1_T18 805 1672 (SEQ ID NO: 865) HUMTEN_PEA_1_T19 805 1672 (SEQ ID NO: 866) HUMTEN_PEA_1_T20 805 1672 (SEQ ID NO. 867) HUMTEN_PEA_1_T23 805 1672 (SEQ ID NO: 868) HUMTEN_PEA_1_T32 805 1672 (SEQ ID NO: 869) HUMTEN_PEA_1_T35 805 1672 (SEQ ID NO: 870) HUMTEN_PEA_1_T36 805 1672 (SEQ ID NO: 871) HUMTEN_PEA_1_T37 805 1672 (SEQ ID NO: 872) HUMTEN_PEA_1_T39 805 1672 (SEQ ID NO: 873) HUMTEN_PEA_1_T40 805 1672 (SEQ ID NO: 874) HUMTEN_PEA_1_T41 805 1672 (SEQ ID NO: 875)

Segment cluster HUMTEN_PEA_(—)1_node_(—)6 (SEQ ID NO: 879) according to the present invention is supported by 21 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HUMTEN_PEA_(—)1_T4 (SEQ ID NO: 857), HUMTEN_PEA_(—)1_T5 (SEQ ID NO: 858) HUMTEN_PEA_(—)1_T5 (SEQ ID NO: 858) (SEQ ID NO: 859), HUMTEN_PEA_(—)1_T6 (SEQ ID NO: 859), HUMTEN_PEA_(—)1_T7 (SEQ ID NO: 860), HUMTEN_PEA_(—)1_T11 (SEQ ID NO: 861), HUMTEN_PEA_(—)1_T14 (SEQ ID NO: 862), HUMTEN_PEA_(—)1_T16 (SEQ ID NO: 863), HUMTEN_PEA_(—)1_T17 (SEQ ID NO: 864), HUMTEN_PEA_(—)1_T18 (SEQ ID NO: 865), HUMTEN_PEA_(—)1_T19 (SEQ ID NO: 866), HUMTEN_PEA_(—)1_T20 (SEQ ID NO. 867), HUMTEN_PEA_(—)1_T23 (SEQ ID NO: 868), HUMTEN_PEA_(—)1_T32 (SEQ ID NO: 869), HUMTEN_PEA_(—)1_T35 (SEQ ID NO: 870), HUMTEN_PEA_(—)1_T36 (SEQ ID NO: 871), HUMTEN_PEA_(—)1_T37 (SEQ ID NO: 872), HUMTEN_PEA_(—)1_T39 (SEQ ID NO: 873), HUMTEN_PEA_(—)1_T40 (SEQ ID NO: 874) and HUMTEN_PEA_(—)1_T41 (SEQ ID NO: 875). Table 67 below describes the starting and ending position of this segment on each transcript.

TABLE 67 Segment location on transcripts Segment Segment Transcript name starting position ending position HUMTEN_PEA_1_T4 1673 1925 (SEQ ID NO: 857) HUMTEN_PEA_1_T5 1673 1925 (SEQ ID NO: 858) HUMTEN_PEA_1_T5 (SEQ ID NO: 858) (SEQ ID NO: 859) HUMTEN_PEA_1_T6 1673 1925 (SEQ ID NO: 859) HUMTEN_PEA_1_T7 1673 1925 (SEQ ID NO: 860) HUMTEN_PEA_1_T11 1673 1925 (SEQ ID NO: 861) HUMTEN_PEA_1_T14 1673 1925 (SEQ ID NO: 862) HUMTEN_PEA_1_T16 1673 1925 (SEQ ID NO: 863) HUMTEN_PEA_1_T17 1673 1925 (SEQ ID NO: 864) HUMTEN_PEA_1_T18 1673 1925 (SEQ ID NO: 865) HUMTEN_PEA_1_T19 1673 1925 (SEQ ID NO: 866) HUMTEN_PEA_1_T20 1673 1925 (SEQ ID NO. 867) HUMTEN_PEA_1_T23 1673 1925 (SEQ ID NO: 868) HUMTEN_PEA_1_T32 1673 1925 (SEQ ID NO: 869) HUMTEN_PEA_1_T35 1673 1925 (SEQ ID NO: 870) HUMTEN_PEA_1_T36 1673 1925 (SEQ ID NO: 871) HUMTEN_PEA_1_T37 1673 1925 (SEQ ID NO: 872) HUMTEN_PEA_1_T39 1673 1925 (SEQ ID NO: 873) HUMTEN_PEA_1_T40 1673 1925 (SEQ ID NO: 874) HUMTEN_PEA_1_T41 1673 1925 (SEQ ID NO: 875)

Segment cluster HUMTEN_PEA_(—)1_node_(—)11 (SEQ ID NO: 880) according to the present invention is supported by 34 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HUMTEN_PEA_(—)1_T4 (SEQ ID NO: 857), HUMTEN_PEA_(—)1_T5 (SEQ ID NO: 858) HUMTEN_PEA_(—)1_T5 (SEQ ID NO: 858) (SEQ ID NO: 859), HUMTEN_PEA_(—)1_T6 (SEQ ID NO: 859), HUMTEN_PEA_(—)1_T7 (SEQ ID NO: 860), HUMTEN_PEA_(—)1_T11 (SEQ ID NO: 861), HUMTEN_PEA_(—)1_T14 (SEQ ID NO: 862), HUMTEN_PEA_(—)1_T16 (SEQ ID NO: 863), HUMTEN_PEA_(—)1_T17 (SEQ ID NO: 864), HUMTEN_PEA_(—)1_T18 (SEQ ID NO: 865), HUMTEN_PEA_(—)1_T19 (SEQ ID NO: 866), HUMTEN_PEA_(—)1_T20 (SEQ ID NO. 867), HUMTEN_PEA_(—)1_T23 (SEQ ID NO: 868), HUMTEN_PEA_(—)1_T32 (SEQ ID NO: 869), HUMTEN_PEA_(—)1_T35 (SEQ ID NO: 870), HUMTEN_PEA_(—)1_T36 (SEQ ID NO: 871), HUMTEN_PEA_(—)1_T37 (SEQ ID NO: 872), HUMTEN_PEA_(—)1_T39 (SEQ ID NO: 873), HUMTEN_PEA_(—)1_T40 (SEQ ID NO: 874) and HUMTEN_PEA_(—)1_T41 (SEQ ID NO: 875). Table 68 below describes the starting and ending position of this segment on each transcript.

TABLE 68 Segment location on transcripts Segment Segment Transcript name starting position ending position HUMTEN_PEA_1_T4 2215 2478 (SEQ ID NO: 857) HUMTEN_PEA_1_T5 2215 2478 (SEQ ID NO: 858) HUMTEN_PEA_1_T5 (SEQ ID NO: 858) (SEQ ID NO: 859) HUMTEN_PEA_1_T6 2215 2478 (SEQ ID NO: 859) HUMTEN_PEA_1_T7 2215 2478 (SEQ ID NO: 860) HUMTEN_PEA_1_T11 2215 2478 (SEQ ID NO: 861) HUMTEN_PEA_1_T14 2215 2478 (SEQ ID NO: 862) HUMTEN_PEA_1_T16 2215 2478 (SEQ ID NO: 863) HUMTEN_PEA_1_T17 2215 2478 (SEQ ID NO: 864) HUMTEN_PEA_1_T18 2215 2478 (SEQ ID NO: 865) HUMTEN_PEA_1_T19 2215 2478 (SEQ ID NO: 866) HUMTEN_PEA_1_T20 2215 2478 (SEQ ID NO. 867) HUMTEN_PEA_1_T23 2215 2478 (SEQ ID NO: 868) HUMTEN_PEA_1_T32 2215 2478 (SEQ ID NO: 869) HUMTEN_PEA_1_T35 2215 2478 (SEQ ID NO: 870) HUMTEN_PEA_1_T36 2215 2478 (SEQ ID NO: 871) HUMTEN_PEA_1_T37 2215 2478 (SEQ ID NO: 872) HUMTEN_PEA_1_T39 2215 2478 (SEQ ID NO: 873) HUMTEN_PEA_1_T40 2215 2478 (SEQ ID NO: 874) HUMTEN_PEA_1_T41 2215 2478 (SEQ ID NO: 875)

Segment cluster HUMTEN_PEA_(—)1_node_(—)12 (SEQ ID NO: 881) according to the present invention is supported by 2 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HUMTEN_PEA_(—)1_T41 (SEQ ID NO: 875). Table 69 below describes the starting and ending position of this segment on each transcript.

TABLE 69 Segment location on transcripts Segment Segment Transcript name starting position ending position HUMTEN_PEA_1_T41 2479 3027 (SEQ ID NO: 875)

Segment cluster HUMTEN_PEA_(—)1_node_(—)16 (SEQ ID NO: 882) according to the present invention is supported by 27 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HUMTEN_PEA_(—)1_T4 (SEQ ID NO: 857), HUMTEN_PEA_(—)1_T5 (SEQ ID NO: 858) HUMTEN_PEA_(—)1_T5 (SEQ ID NO: 858) (SEQ ID NO: 859), HUMTEN_PEA_(—)1_T6 (SEQ ID NO: 859), HUMTEN_PEA_(—)1_T7 (SEQ ID NO: 860), HUMTEN_PEA_(—)1_T11 (SEQ ID NO: 861), HUMTEN_PEA_(—)1_T14 (SEQ ID NO: 862), HUMTEN_PEA_(—)1_T16 (SEQ ID NO: 863), HUMTEN_PEA_(—)1_T17 (SEQ ID NO: 864), HUMTEN_PEA_(—)1_T18 (SEQ ID NO: 865), HUMTEN_PEA_(—)1_T19 (SEQ ID NO: 866), HUMTEN_PEA_(—)1_T20 (SEQ ID NO. 867), HUMTEN_PEA_(—)1_T23 (SEQ ID NO: 868), HUMTEN_PEA_(—)1_T32 (SEQ ID NO: 869), HUMTEN_PEA_(—)1_T35 (SEQ ID NO: 870), HUMTEN_PEA_(—)1_T36 (SEQ ID NO: 871), HUMTEN_PEA_(—)1_T37 (SEQ ID NO: 872), HUMTEN_PEA_(—)1_T39 (SEQ ID NO: 873) and HUMTEN_PEA_(—)1_T40 (SEQ ID NO: 874). Table 70 below describes the starting and ending position of this segment on each transcript.

TABLE 70 Segment location on transcripts Segment Segment Transcript name starting position ending position HUMTEN_PEA_1_T4 2595 2751 (SEQ ID NO: 857) HUMTEN_PEA_1_T5 2595 2751 (SEQ ID NO: 858) HUMTEN_PEA_1_T5 (SEQ ID NO: 858) (SEQ ID NO: 859) HUMTEN_PEA_1_T6 2595 2751 (SEQ ID NO: 859) HUMTEN_PEA_1_T7 2595 2751 (SEQ ID NO: 860) HUMTEN_PEA_1_T11 2595 2751 (SEQ ID NO: 861) HUMTEN_PEA_1_T14 2595 2751 (SEQ ID NO: 862) HUMTEN_PEA_1_T16 2595 2751 (SEQ ID NO: 863) HUMTEN_PEA_1_T17 2595 2751 (SEQ ID NO: 864) HUMTEN_PEA_1_T18 2595 2751 (SEQ ID NO: 865) HUMTEN_PEA_1_T19 2595 2751 (SEQ ID NO: 866) HUMTEN_PEA_1_T20 2595 2751 (SEQ ID NO. 867) HUMTEN_PEA_1_T23 2595 2751 (SEQ ID NO: 868) HUMTEN_PEA_1_T32 2595 2751 (SEQ ID NO: 869) HUMTEN_PEA_1_T35 2595 2751 (SEQ ID NO: 870) HUMTEN_PEA_1_T36 2595 2751 (SEQ ID NO: 871) HUMTEN_PEA_1_T37 2595 2751 (SEQ ID NO: 872) HUMTEN_PEA_1_T39 2595 2751 (SEQ ID NO: 873) HUMTEN_PEA_1_T40 2595 2751 (SEQ ID NO: 874)

Segment cluster HUMTEN_PEA_(—)1_node_(—)19 (SEQ ID NO: 883) according to the present invention is supported by 29 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HUMTEN_PEA_(—)1_T4 (SEQ ID NO: 857), HUMTEN_PEA_(—)1_T5 (SEQ ID NO: 858) HUMTEN_PEA_(—)1_T5 (SEQ ID NO: 858) (SEQ ID NO: 859), HUMTEN_PEA_(—)1_T6 (SEQ ID NO: 859), HUMTEN_PEA_(—)1_T7 (SEQ ID NO: 860), HUMTEN_PEA_(—)1_T11 (SEQ ID NO: 861), HUMTEN_PEA_(—)1_T14 (SEQ ID NO: 862), HUMTEN_PEA_(—)1_T16 (SEQ ID NO: 863), HUMTEN_PEA_(—)1_T17 (SEQ ID NO: 864), HUMTEN_PEA_(—)1_T18 (SEQ ID NO: 865), HUMTEN_PEA_(—)1_T19 (SEQ ID NO: 866), HUMTEN_PEA_(—)1_T20 (SEQ ID NO. 867), HUMTEN_PEA_(—)1_T23 (SEQ ID NO: 868), HUMTEN_PEA_(—)1_T32 (SEQ ID NO: 869), HUMTEN_PEA_(—)1_T35 (SEQ ID NO: 870), HUMTEN_PEA_(—)1_T36 (SEQ ID NO: 871), HUMTEN_PEA_(—)1_T37 (SEQ ID NO: 872) and HUMTEN_PEA_(—)1_T39 (SEQ ID NO: 873). Table 71 below describes the starting and ending position of this segment on each transcript.

TABLE 71 Segment location on transcripts Segment Segment Transcript name starting position ending position HUMTEN_PEA_1_T4 2752 3021 (SEQ ID NO: 857) HUMTEN_PEA_1_T5 2752 3021 (SEQ ID NO: 858) HUMTEN_PEA_1_T5 (SEQ ID NO: 858) (SEQ ID NO: 859) HUMTEN_PEA_1_T6 2752 3021 (SEQ ID NO: 859) HUMTEN_PEA_1_T7 2752 3021 (SEQ ID NO: 860) HUMTEN_PEA_1_T11 2752 3021 (SEQ ID NO: 861) HUMTEN_PEA_1_T14 2752 3021 (SEQ ID NO: 862) HUMTEN_PEA_1_T16 2752 3021 (SEQ ID NO: 863) HUMTEN_PEA_1_T17 2752 3021 (SEQ ID NO: 864) HUMTEN_PEA_1_T18 2752 3021 (SEQ ID NO: 865) HUMTEN_PEA_1_T19 2752 3021 (SEQ ID NO: 866) HUMTEN_PEA_1_T20 2752 3021 (SEQ ID NO. 867) HUMTEN_PEA_1_T23 2752 3021 (SEQ ID NO: 868) HUMTEN_PEA_1_T32 2752 3021 (SEQ ID NO: 869) HUMTEN_PEA_1_T35 2752 3021 (SEQ ID NO: 870) HUMTEN_PEA_1_T36 2752 3021 (SEQ ID NO: 871) HUMTEN_PEA_1_T37 2752 3021 (SEQ ID NO: 872) HUMTEN_PEA_1_T39 2752 3021 (SEQ ID NO: 873)

Segment cluster HUMTEN_PEA_(—)1_node_(—)23 (SEQ ID NO: 884) according to the present invention is supported by 1 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HUMTEN_PEA_(—)1_T39 (SEQ ID NO: 873). Table 72 below describes the starting and ending position of this segment on each transcript.

TABLE 72 Segment location on transcripts Segment Segment Transcript name starting position ending position HUMTEN_PEA_1_T39 3208 3335 (SEQ ID NO: 873)

Segment cluster HUMTEN_PEA_(—)1_node_(—)27 (SEQ ID NO: 885) according to the present invention is supported by 43 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HUMTEN_PEA_(—)1_T4 (SEQ ID NO: 857), HUMTEN_PEA_(—)1_T5 (SEQ ID NO: 858) HUMTEN_PEA_(—)1_T5 (SEQ 11D NO: 858) (SEQ ID NO: 859), HUMTEN_PEA_(—)1_T6 (SEQ ID NO: 859), HUMTEN_PEA_(—)1_T7 (SEQ ID NO: 860), HUMTEN_PEA_(—)1_T11 (SEQ ID NO: 861), HUMTEN_PEA_(—)1_T14 (SEQ ID NO: 862), HUMTEN_PEA_(—)1_T16 (SEQ ID NO: 863), HUMTEN_PEA_(—)1_T17 (SEQ ID NO: 864), HUMTEN_PEA_(—)1_T18 (SEQ ID NO: 865), HUMTEN_PEA_(—)1_T19 (SEQ ID NO: 866), HUMTEN_PEA_(—)1_T20 (SEQ ID NO. 867), HUMTEN_PEA_(—)1_T23 (SEQ ID NO: 868), HUMTEN_PEA_(—)1_T32 (SEQ ID NO: 869), HUMTEN_PEA_(—)1_T35 (SEQ ID NO: 870), HUMTEN_PEA_(—)1_T36 (SEQ ID NO: 871) and HUMTEN_PEA_(—)1_T37 (SEQ ID NO: 872). Table 73 below describes the starting and ending position of this segment on each transcript.

TABLE 73 Segment location on transcripts Segment Segment Transcript name starting position ending position HUMTEN_PEA_1_T4 3298 3561 (SEQ ID NO: 857) HUMTEN_PEA_1_T5 3298 3561 (SEQ ID NO: 858) HUMTEN_PEA_1_T5 (SEQ ID NO: 858) (SEQ ID NO: 859) HUMTEN_PEA_1_T6 3298 3561 (SEQ ID NO: 859) HUMTEN_PEA_1_T7 3298 3561 (SEQ ID NO: 860) HUMTEN_PEA_1_T11 3298 3561 (SEQ ID NO: 861) HUMTEN_PEA_1_T14 3298 3561 (SEQ ID NO: 862) HUMTEN_PEA_1_T16 3298 3561 (SEQ ID NO: 863) HUMTEN_PEA_1_T17 3298 3561 (SEQ ID NO: 864) HUMTEN_PEA_1_T18 3298 3561 (SEQ ID NO: 865) HUMTEN_PEA_1_T19 3298 3561 (SEQ ID NO: 866) HUMTEN_PEA_1_T20 3298 3561 (SEQ ID NO. 867) HUMTEN_PEA_1_T23 3298 3561 (SEQ ID NO: 868) HUMTEN_PEA_1_T32 3298 3561 (SEQ ID NO: 869) HUMTEN_PEA_1_T35 3298 3561 (SEQ ID NO: 870) HUMTEN_PEA_1_T36 3298 3561 (SEQ ID NO: 871) HUMTEN_PEA_1_T37 3298 3561 (SEQ ID NO: 872)

Segment cluster HUMTEN_PEA_(—)1_node_(—)28 (SEQ ID NO: 886) according to the present invention is supported by 2 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HUMTEN_PEA_(—)1_T37 (SEQ ID NO: 872). Table 74 below describes the starting and ending position of this segment on each transcript.

TABLE 74 Segment location on transcripts Segment Segment Transcript name starting position ending position HUMTEN_PEA_1_T37 3562 3762 (SEQ ID NO: 872)

Segment cluster HUMTEN_PEA_(—)1_node_(—)30 (SEQ ID NO: 887) according to the present invention is supported by 19 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HUMTEN_PEA_(—)1_T4 (SEQ ID NO: 857), HUMTEN_PEA_(—)1_T5 (SEQ ID NO: 858), HUMTEN_PEA_(—)1_T6 (SEQ ID NO: 859), HUMTEN_PEA_(—)1_T7 (SEQ ID NO: 860), HUMTEN_PEA_(—)1_T11 (SEQ ID NO: 861), HUMTEN_PEA_(—)1_T14 (SEQ ID NO: 862), HUMTEN_PEA_(—)1_T16 (SEQ ID NO: 863), HUMTEN_PEA_(—)1_T17 (SEQ ID NO: 864), HUMTEN_PEA_(—)1_T20 (SEQ ID NO. 867), HUMTEN_PEA_(—)1_T23 (SEQ ID NO: 868), HUMTEN_PEA_(—)1_T32 (SEQ ID NO: 869), HUMTEN_PEA_(—)1_T35 (SEQ ID NO: 870) and HUMTEN_PEA_(—)1_T36 (SEQ ID NO: 871). Table 75 below describes the starting and ending position of this segment on each transcript.

TABLE 75 Segment location on transcripts Segment Segment Transcript starting ending name position position HUMTEN_PEA_1_T4 3562 3834 (SEQ ID NO: 857) HUMTEN_PEA_1_T5 3562 3834 (SEQ ID NO: 858) HUMTEN_PEA_1_T6 3562 3834 (SEQ ID NO: 859) HUMTEN_PEA_1_T7 3562 3834 (SEQ ID NO: 860) HUMTEN_PEA_1_T11 3562 3834 (SEQ ID NO: 861) HUMTEN_PEA_1_T14 3562 3834 (SEQ ID NO: 862) HUMTEN_PEA_1_T16 3562 3834 (SEQ ID NO: 863) HUMTEN_PEA_1_T17 3562 3834 (SEQ ID NO: 864) HUMTEN_PEA_1_T20 3562 3834 (SEQ ID NO. 867) HUMTEN_PEA_1_T23 3562 3834 (SEQ ID NO: 868) HUMTEN_PEA_1_T32 3562 3834 (SEQ ID NO: 869) HUMTEN_PEA_1_T35 3562 3834 (SEQ ID NO: 870) HUMTEN_PEA_1_T36 3562 3834 (SEQ ID NO: 871)

Segment cluster HUMTEN_PEA_(—)1_node_(—)32 (SEQ ID NO: 888) according to the present invention is supported by 22 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HUMTEN_PEA_(—)1_T4 (SEQ ID NO: 857), HUMTEN_PEA_(—)1_T5 (SEQ ID NO: 858), HUMTEN_PEA_(—)1_T6 (SEQ ID NO: 859), HUMTEN_PEA_(—)1_T7 (SEQ ID NO: 860), HUMTEN_PEA_(—)1_T11 (SEQ ID NO: 861), HUMTEN_PEA_(—)1_T16 (SEQ ID NO: 863), HUMTEN_PEA_(—)1_T17 (SEQ ID NO: 864), HUMTEN_PEA_(—)1_T20 (SEQ ID NO. 867), HUMTEN_PEA_(—)1_T23 (SEQ ID NO: 868), HUMTEN_PEA_(—)1_T32 (SEQ ID NO: 869), HUMTEN_PEA_(—)1_T35 (SEQ ID NO: 870) and HUMTEN_PEA_(—)1_T36 (SEQ ID NO: 871). Table 76 below describes the starting and ending position of this segment on each transcript.

TABLE 76 Segment location on transcripts Segment Segment Transcript starting ending name position position HUMTEN_PEA_1_T4 3835 4107 (SEQ ID NO: 857) HUMTEN_PEA_1_T5 3835 4107 (SEQ ID NO: 858) HUMTEN_PEA_1_T6 3835 4107 (SEQ ID NO: 859) HUMTEN_PEA_1_T7 3835 4107 (SEQ ID NO: 860) HUMTEN_PEA_1_T11 3835 4107 (SEQ ID NO: 861) HUMTEN_PEA_1_T16 3835 4107 (SEQ ID NO: 863) HUMTEN_PEA_1_T17 3835 4107 (SEQ ID NO: 864) HUMTEN_PEA_1_T20 3835 4107 (SEQ ID NO. 867) HUMTEN_PEA_1_T23 3835 4107 (SEQ ID NO: 868) HUMTEN_PEA_1_T32 3835 4107 (SEQ ID NO: 869) HUMTEN_PEA_1_T35 3835 4107 (SEQ ID NO: 870) HUMTEN_PEA_1_T36 3835 4107 (SEQ ID NO: 871)

Segment cluster HUMTEN_PEA_(—)1_node_(—)33 (SEQ ID NO: 889) according to the present invention is supported by 2 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HUMTEN_PEA_(—)1_T36 (SEQ ID NO: 871). Table 77 below describes the starting and ending position of this segment on each transcript.

TABLE 77 Segment location on transcripts Segment Segment Transcript starting ending name position position HUMTEN_PEA_1_T36 4108 4463 (SEQ ID NO: 871)

Segment cluster HUMTEN_PEA_(—)1_node_(—)35 (SEQ ID NO: 890) according to the present invention is supported by 21 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HUMTEN_PEA_(—)1_T4 (SEQ ID NO: 857), HUMTEN_PEA_(—)1_T5 (SEQ ID NO: 858), HUMTEN_PEA_(—)1_T6 (SEQ ID NO: 859), HUMTEN_PEA_(—)1_T7 (SEQ ID NO: 860), HUMTEN_PEA_(—)1_T16 (SEQ ID NO: 863), HUMTEN_PEA_(—)1_T17 (SEQ ID NO: 864), HUMTEN_PEA_(—)1_T20 (SEQ ID NO.

867), HUMTEN_PEA_(—)1_T23 (SEQ ID NO: 868), HUMTEN_PEA_(—)1_T32 (SEQ ID NO: 869) and HUMTEN_PEA_(—)1_T35 (SEQ ID NO: 870). Table 78 below describes the starting and ending position of this segment on each transcript.

TABLE 78 Segment location on transcripts Segment Segment Transcript starting ending name position position HUMTEN_PEA_1_T4 4108 4380 (SEQ ID NO: 857) HUMTEN_PEA_1_T5 4108 4380 (SEQ ID NO: 858) HUMTEN_PEA_1_T6 4108 4380 (SEQ ID NO: 859) HUMTEN_PEA_1_T7 4108 4380 (SEQ ID NO: 860) HUMTEN_PEA_1_T16 4108 4380 (SEQ ID NO: 863) HUMTEN_PEA_1_T17 4108 4380 (SEQ ID NO: 864) HUMTEN_PEA_1_T20 4108 4380 (SEQ ID NO. 867) HUMTEN_PEA_1_T23 4108 4380 (SEQ ID NO: 868) HUMTEN_PEA_1_T32 4108 4380 (SEQ ID NO: 869) HUMTEN_PEA_1_T35 4108 4380 (SEQ ID NO: 870)

Segment cluster HUMTEN_PEA_(—)1_node_(—)38 (SEQ ID NO: 891) according to the present invention is supported by 22 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HUMTEN_PEA_(—)1_T4 (SEQ ID NO: 857), HUMTEN_PEA_(—)1_T5 (SEQ ID NO: 858), HUMTEN_PEA_(—)1_T6 (SEQ ID NO: 859), HUMTEN_PEA_(—)1_T7 (SEQ ID NO: 860), HUMTEN_PEA_(—)1_T11 (SEQ ID NO: 861), HUMTEN_PEA_(—)1_T17 (SEQ ID NO: 864), HUMTEN_PEA_(—)1_T20 (SEQ ID NO. 867), HUMTEN_PEA_(—)1_T23 (SEQ ID NO: 868) and HUMTEN_PEA_(—)1_T32 (SEQ ID NO: 869). Table 79 below describes the starting and ending position of this segment on each transcript.

TABLE 79 Segment location on transcripts Segment Segment Transcript starting ending name position position HUMTEN_PEA_1_T4 4381 4653 (SEQ ID NO: 857) HUMTEN_PEA_1_T5 4381 4653 (SEQ ID NO: 858) HUMTEN_PEA_1_T6 4381 4653 (SEQ ID NO: 859) HUMTEN_PEA_1_T7 4381 4653 (SEQ ID NO: 860) HUMTEN_PEA_1_T11 4108 4380 (SEQ ID NO: 861) HUMTEN_PEA_1_T17 4381 4653 (SEQ ID NO: 864) HUMTEN_PEA_1_T20 4381 4653 (SEQ ID NO. 867) HUMTEN_PEA_1_T23 4381 4653 (SEQ ID NO: 868) HUMTEN_PEA_1_T32 4381 4653 (SEQ ID NO: 869)

Segment cluster HUMTEN_PEA_(—)1_node_(—)40 (SEQ ID NO: 892) according to the present invention is supported by 22 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HUMTEN_PEA_(—)1_T4 (SEQ ID NO: 857), HUMTEN_PEA_(—)1_T5 (SEQ ID NO: 858), HUMTEN_PEA_(—)1_T6 (SEQ ID NO: 859), HUMTEN_PEA_(—)1_T7 (SEQ ID NO: 860), HUMTEN_PEA_(—)1_T11 (SEQ ID NO: 861), HUMTEN_PEA_(—)1_T14 (SEQ ID NO: 862), HUMTEN_PEA_(—)1_T17 (SEQ ID NO: 864), HUMTEN_PEA_(—)1_T20 (SEQ ID NO. 867), HUMTEN_PEA_(—)1_T23 (SEQ ID NO: 868) and HUMTEN_PEA_(—)1_T32 (SEQ ID NO: 869). Table 80 below describes the starting and ending position of this segment on each transcript.

TABLE 80 Segment location on transcripts Segment Segment Transcript starting ending name position position HUMTEN_PEA_1_T4 4654 4926 (SEQ ID NO: 857) HUMTEN_PEA_1_T5 4654 4926 (SEQ ID NO: 858) HUMTEN_PEA_1_T6 4654 4926 (SEQ ID NO: 859) HUMTEN_PEA_1_T7 4654 4926 (SEQ ID NO: 860) HUMTEN_PEA_1_T11 4381 4653 (SEQ ID NO: 861) HUMTEN_PEA_1_T14 3835 4107 (SEQ ID NO: 862) HUMTEN_PEA_1_T17 4654 4926 (SEQ ID NO: 864) HUMTEN_PEA_1_T20 4654 4926 (SEQ ID NO. 867) HUMTEN_PEA_1_T23 4654 4926 (SEQ ID NO: 868) HUMTEN_PEA_1_T32 4654 4926 (SEQ ID NO: 869)

Segment cluster HUMTEN_PEA_(—)1_node_(—)42 (SEQ ID NO: 893) according to the present invention is supported by 4 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HUMTEN_PEA_(—)1_T4 (SEQ ID NO: 857) and HUMTEN_PEA_(—)1_T5 (SEQ ID NO: 858). Table 81 below describes the starting and ending position of this segment on each transcript.

TABLE 81 Segment location on transcripts Segment Segment Transcript starting ending name position position HUMTEN_PEA_1_T4 4927 5202 (SEQ ID NO: 857) HUMTEN_PEA_1_T5 4927 5202 (SEQ ID NO: 858)

Segment cluster HUMTEN_PEA_(—)1_node_(—)43 (SEQ ID NO: 894) according to the present invention is supported by 27 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HUMTEN_(—)1_PEA_(—)1_T5 (SEQ ID NO: 858). Table 82 below describes the starting and ending position of this segment on each transcript.

TABLE 82 Segment location on transcripts Segment Segment Transcript starting ending name position position HUMTEN_PEA_1_T5 5203 9409 (SEQ ID NO: 858)

Segment cluster HUMTEN_PEA_(—)1_node_(—)44 (SEQ ID NO: 895) according to the present invention is supported by 15 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HUMTEN_PEA_(—)1_T4 (SEQ ID NO: 857), HUMTEN_PEA_(—)1_T5 (SEQ ID NO: 858), HUMTEN_PEA_(—)1_T6 (SEQ ID NO: 859), HUMTEN_PEA_(—)1_T11 (SEQ ID NO: 861), HUMTEN_PEA_(—)1_T14 (SEQ ID NO: 862), HUMTEN_PEA_(—)1_T17 (SEQ ID NO: 864), HUMTEN_PEA_(—)1_T20 (SEQ ID NO. 867), HUMTEN_PEA_(—)1_T23 (SEQ ID NO: 868) and HUMTEN_PEA_(—)1_T32 (SEQ ID NO: 869). Table 83 below describes the starting and ending position of this segment on each transcript.

TABLE 83 Segment location on transcripts Segment Segment Transcript starting ending name position position HUMTEN_PEA_1_T4 5203 5475 (SEQ ID NO: 857) HUMTEN_PEA_1_T5 9410 9682 (SEQ ID NO: 858) HUMTEN_PEA_1_T6 4927 5199 (SEQ ID NO: 859) HUMTEN_PEA_1_T11 4654 4926 (SEQ ID NO: 861) HUMTEN_PEA_1_T14 4108 4380 (SEQ ID NO: 862) HUMTEN_PEA_1_T17 4927 5199 (SEQ ID NO: 864) HUMTEN_PEA_1_T20 4927 5199 (SEQ ID NO. 867) HUMTEN_PEA_1_T23 4927 5199 (SEQ ID NO: 868) HUMTEN_PEA_1_T32 4927 5199 (SEQ ID NO: 869)

Segment cluster HUMTEN_PEA_(—)1_node_(—)45 (SEQ ID NO: 896) according to the present invention is supported by 17 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HUMTEN_PEA_(—)1_T6 (SEQ ID NO: 859). Table 84 below describes the starting and ending position of this segment on each transcript.

TABLE 84 Segment location on transcripts Segment Segment Transcript starting ending name position position HUMTEN_PEA_1_T6 5200 6776 (SEQ ID NO: 859)

Segment cluster HUMTEN_PEA_(—)1_node_(—)46 (SEQ ID NO: 897) according to the present invention is supported by 40 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HUMTEN_PEA_(—)1_T4 (SEQ ID NO: 857), HUMTEN_PEA_(—)1_T5 (SEQ ID NO: 858), HUMTEN_PEA_(—)1_T6 (SEQ ID NO: 859), HUMTEN_PEA_(—)1_T7 (SEQ ID NO: 860), HUMTEN_PEA_(—)1_T11 (SEQ ID NO: 861), HUMTEN_PEA_(—)1_T14 (SEQ ID NO: 862), HUMTEN_PEA_(—)1_T17 (SEQ ID NO: 864), HUMTEN_PEA_(—)1_T18 (SEQ ID NO: 865), HUMTEN_PEA_(—)1_T20 (SEQ ID NO. 867), HUMTEN_PEA_(—)1_T23 (SEQ ID NO: 868) and HUMTEN_PEA_(—)1_T32 (SEQ ID NO: 869). Table 85 below describes the starting and ending position of this segment on each transcript.

TABLE 85 Segment location on transcripts Segment Segment Transcript starting ending name position position HUMTEN_PEA_1_T4 5476 5748 (SEQ ID NO: 857) HUMTEN_PEA_1_T5 9683 9955 (SEQ ID NO: 858) HUMTEN_PEA_1_T6 6777 7049 (SEQ ID NO: 859) HUMTEN_PEA_1_T7 4927 5199 (SEQ ID NO: 860) HUMTEN_PEA_1_T11 4927 5199 (SEQ ID NO: 861) HUMTEN_PEA_1_T14 4381 4653 (SEQ ID NO: 862) HUMTEN_PEA_1_T17 5200 5472 (SEQ ID NO: 864) HUMTEN_PEA_1_T18 3562 3834 (SEQ ID NO: 865) HUMTEN_PEA_1_T20 5200 5472 (SEQ ID NO. 867) HUMTEN_PEA_1_T23 5200 5472 (SEQ ID NO: 868) HUMTEN_PEA_1_T32 5200 5472 (SEQ ID NO: 869)

Segment cluster HUMTEN_PEA_(—)1_node_(—)47 (SEQ ID NO: 898) according to the present invention is supported by 8 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HUMTEN_PEA_(—)1_T32 (SEQ ID NO: 869). Table 86 below describes the starting and ending position of this segment on each transcript.

TABLE 86 Segment location on transcripts Segment Segment Transcript starting ending name position position HUMTEN_PEA_1_T32 5473 6320 (SEQ ID NO: 869)

Segment cluster HUMTEN_PEA_(—)1_node_(—)49 (SEQ ID NO: 899) according to the present invention is supported by 59 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HUMTEN_PEA_(—)1_T4 (SEQ ID NO: 857), HUMTEN_PEA_(—)1_T5 (SEQ ID NO: 858), HUMTEN_PEA_(—)1_T6 (SEQ ID NO: 859), HUMTEN_PEA_(—)1_T7 (SEQ ID NO: 860), HUMTEN_PEA_(—)1_T11 (SEQ ID NO: 861), HUMTEN_PEA_(—)1_T14 (SEQ ID NO: 862), HUMTEN_PEA_(—)1_T16 (SEQ ID NO: 863), HUMTEN_PEA_(—)1_T7 (SEQ ID NO: 864), HUMTEN_PEA_(—)1_T18 (SEQ ID NO: 865), HUMTEN_PEA_(—)1_T19 (SEQ ID NO: 866), HUMTEN_PEA_(—)1_T20 (SEQ ID NO. 867) and HUMTEN_PEA_(—)1_T23 (SEQ ID NO: 868). Table 87 below describes the starting and ending position of this segment on each transcript.

TABLE 87 Segment location on transcripts Segment Segment Transcript starting ending name position position HUMTEN_PEA_1_T4 5749 5871 (SEQ ID NO: 857) HUMTEN_PEA_1_T5 9956 10078 (SEQ ID NO: 858) HUMTEN_PEA_1_T6 7050 7172 (SEQ ID NO: 859) HUMTEN_PEA_1_T7 5200 5322 (SEQ ID NO: 860) HUMTEN_PEA_1_T11 5200 5322 (SEQ ID NO: 861) HUMTEN_PEA_1_T14 4654 4776 (SEQ ID NO: 862) HUMTEN_PEA_1_T16 4381 4503 (SEQ ID NO: 863) HUMTEN_PEA_1_T17 5473 5595 (SEQ ID NO: 864) HUMTEN_PEA_1_T18 3835 3957 (SEQ ID NO: 865) HUMTEN_PEA_1_T19 3562 3684 (SEQ ID NO: 866) HUMTEN_PEA_1_T20 5473 5595 (SEQ ID NO. 867) HUMTEN_PEA_1_T23 5473 5595 (SEQ ID NO: 868)

Segment cluster HUMTEN_PEA_(—)1_node_(—)51 (SEQ ID NO: 900) according to the present invention is supported by 74 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HUMTEN_PEA_(—)1_T4 (SEQ ID NO: 857), HUMTEN_PEA_(—)1_T5 (SEQ ID NO: 858), HUMTEN_PEA_(—)1_T6 (SEQ ID NO: 859), HUMTEN_PEA_(—)1_T7 (SEQ ID NO: 860), HUMTEN_PEA_(—)1_T11 (SEQ ID NO: 861), HUMTEN_PEA_(—)1_T14 (SEQ ID NO: 862), HUMTEN_PEA_(—)1_T16 (SEQ ID NO: 863), HUMTEN_PEA_(—)1_T7 (SEQ ID NO: 864), HUMTEN_PEA_(—)1_T18 (SEQ ID NO: 865), HUMTEN_PEA_(—)1_T19 (SEQ ID NO: 866), HUMTEN_PEA_(—)1_T20 (SEQ ID NO. 867) and HUMTEN_PEA_(—)1_T23 (SEQ ID NO: 868). Table 88 below describes the starting and ending position of this segment on each transcript.

TABLE 88 Segment location on transcripts Segment Segment Transcript starting ending name position position HUMTEN_PEA_1_T4 5872 6015 (SEQ ID NO: 857) HUMTEN_PEA_1_T5 10079 10222 (SEQ ID NO: 858) HUMTEN_PEA_1_T6 7173 7316 (SEQ ID NO: 859) HUMTEN_PEA_1_T7 5323 5466 (SEQ ID NO: 860) HUMTEN_PEA_1_T11 5323 5466 (SEQ ID NO: 861) HUMTEN_PEA_1_T14 4777 4920 (SEQ ID NO: 862) HUMTEN_PEA_1_T16 4504 4647 (SEQ ID NO: 863) HUMTEN_PEA_1_T17 5596 5739 (SEQ ID NO: 864) HUMTEN_PEA_1_T18 3958 4101 (SEQ ID NO: 865) HUMTEN_PEA_1_T19 3685 3828 (SEQ ID NO: 866) HUMTEN_PEA_1_T20 5596 5739 (SEQ ID NO. 867) HUMTEN_PEA_1_T23 5596 5739 (SEQ ID NO: 868)

Segment cluster HUMTEN_PEA_(—)1_node_(—)56 (SEQ ID NO: 901) according to the present invention is supported by 84 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HUMTEN_PEA_(—)1_T4 (SEQ ID NO: 857), HUMTEN_PEA_(—)1_T5 (SEQ ID NO: 858), HUMTEN_PEA_(—)1_T6 (SEQ ID NO: 859), HUMTEN_PEA_(—)1_T7 (SEQ ID NO: 860), HUMTEN_PEA_(—)1_T11 (SEQ ID NO: 861), HUMTEN_PEA_(—)1_T14 (SEQ ID NO: 862), HUMTEN_PEA_(—)1_T16 (SEQ ID NO: 863), HUMTEN_PEA_(—)1_T17 (SEQ ID NO: 864), HUMTEN_PEA_(—)1_T18 (SEQ ID NO: 865), HUMTEN_PEA_(—)1_T19 (SEQ ID NO: 866), HUMTEN_PEA_(—)1_T20 (SEQ ID NO. 867) and HUMTEN_PEA_(—)1_T23 (SEQ ID NO: 868). Table 89 below describes the starting and ending position of this segment on each transcript.

TABLE 89 Segment location on transcripts Segment Segment Transcript starting ending name position position HUMTEN_PEA_1_T4 6136 6261 (SEQ ID NO: 857) HUMTEN_PEA_1_T5 10343 10468 (SEQ ID NO: 858) HUMTEN_PEA_1_T6 7437 7562 (SEQ ID NO: 859) HUMTEN_PEA_1_T7 5587 5712 (SEQ ID NO: 860) HUMTEN_PEA_1_T11 5587 5712 (SEQ ID NO: 861) HUMTEN_PEA_1_T14 5041 5166 (SEQ ID NO: 862) HUMTEN_PEA_1_T16 4768 4893 (SEQ ID NO: 863) HUMTEN_PEA_1_T17 5860 5985 (SEQ ID NO: 864) HUMTEN_PEA_1_T18 4222 4347 (SEQ ID NO: 865) HUMTEN_PEA_1_T19 3949 4074 (SEQ ID NO: 866) HUMTEN_PEA_1_T20 5860 5985 (SEQ ID NO. 867) HUMTEN_PEA_1_T23 5860 5985 (SEQ ID NO: 868)

Segment cluster HUMTEN_PEA_(—)1_node_(—)65 (SEQ ID NO: 902) according to the present invention is supported by 103 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HUMTEN_PEA_(—)1_T4 (SEQ ID NO: 857), HUMTEN_PEA_(—)1_T5 (SEQ ID NO: 858), HUMTEN_PEA_(—)1_T6 (SEQ ID NO: 859), HUMTEN_PEA_(—)1_T7 (SEQ ID NO: 860), HUMTEN_PEA_(—)1_T11 (SEQ ID NO: 861), HUMTEN_PEA_(—)1_T14 (SEQ ID NO: 862), HUMTEN_PEA_(—)1_T6 (SEQ ID NO: 863), HUMTEN_PEA_(—)1_T17 (SEQ ID NO: 864), HUMTEN_PEA_(—)1_T18 (SEQ ID NO: 865), HUMTEN_PEA_(—)1_T19 (SEQ ID NO: 866), HUMTEN_PEA_(—)1_T20 (SEQ ID NO. 867) and HUMTEN_PEA_(—)1_T23 (SEQ ID NO: 868). Table 90 below describes the starting and ending position of this segment on each transcript.

TABLE 90 Segment location on transcripts Segment Segment Transcript starting ending name position position HUMTEN_PEA_1_T4 6411 6543 (SEQ ID NO: 857) HUMTEN_PEA_1_T5 10618 10750 (SEQ ID NO: 858) HUMTEN_PEA_1_T6 7712 7844 (SEQ ID NO: 859) HUMTEN_PEA_1_T7 5862 5994 (SEQ ID NO: 860) HUMTEN_PEA_1_T11 5862 5994 (SEQ ID NO: 861) HUMTEN_PEA_1_T14 5316 5448 (SEQ ID NO: 862) HUMTEN_PEA_1_T16 5043 5175 (SEQ ID NO: 863) HUMTEN_PEA_1_T17 6135 6267 (SEQ ID NO: 864) HUMTEN_PEA_1_T18 4497 4629 (SEQ ID NO: 865) HUMTEN_PEA_1_T19 4224 4356 (SEQ ID NO: 866) HUMTEN_PEA_1_T20 6135 6267 (SEQ ID NO. 867) HUMTEN_PEA_1_T23 6135 6267 (SEQ ID NO: 868)

Segment cluster HUMTEN_PEA_(—)1_node_(—)71 (SEQ ID NO: 903) according to the present invention is supported by 6 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HUMTEN_PEA_(—)1_T17 (SEQ ID NO: 864). Table 91 below describes the starting and ending position of this segment on each transcript.

TABLE 91 Segment location on transcripts Segment Segment Transcript starting ending name position position HUMTEN_PEA_1_T17 6420 7216 (SEQ ID NO: 864)

Segment cluster HUMTEN_PEA_(—)1_node_(—)73 (SEQ ID NO: 904) according to the present invention is supported by 4 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HUMTEN_PEA_(—)1_T23 (SEQ ID NO: 868). Table 92 below describes the starting and ending position of this segment on each transcript.

TABLE 92 Segment location on transcripts Segment Segment Transcript starting ending name position position HUMTEN_PEA_1_T23 6517 6843 (SEQ ID NO: 868)

Segment cluster HUMTEN_PEA_(—)1_node_(—)76 (SEQ ID NO: 905) according to the present invention is supported by 124 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HUMTEN_PEA_(—)1_T4 (SEQ ID NO: 857), HUMTEN_PEA_(—)1_T5 (SEQ ID NO: 858), HUMTEN_PEA_(—)1_T6 (SEQ ID NO: 859), HUMTEN_PEA_(—)1_T7 (SEQ ID NO: 860), HUMTEN_PEA_(—)1_T11 (SEQ ID NO: 861), HUMTEN_PEA_(—)1_T14 (SEQ ID NO: 862), HUMTEN_PEA_(—)1_T16 (SEQ ID NO: 863), HUMTEN_PEA_(—)1_T17 (SEQ ID NO: 864), HUMTEN_PEA_(—)1_T18 (SEQ ID NO: 865) and HUMTEN_PEA_(—)1_T19 (SEQ ID NO: 866). Table 93 below describes the starting and ending position of this segment on each transcript.

TABLE 93 Segment location on transcripts Segment Segment Transcript starting ending name position position HUMTEN_PEA_1_T4 6793 6954 (SEQ ID NO: 857) HUMTEN_PEA_1_T5 11000 11161 (SEQ ID NO: 858) HUMTEN_PEA_1_T6 8094 8255 (SEQ ID NO: 859) HUMTEN_PEA_1_T7 6244 6405 (SEQ ID NO: 860) HUMTEN_PEA_1_T11 6244 6405 (SEQ ID NO: 861) HUMTEN_PEA_1_T14 5698 5859 (SEQ ID NO: 862) HUMTEN_PEA_1_T16 5425 5586 (SEQ ID NO: 863) HUMTEN_PEA_1_T17 7314 7475 (SEQ ID NO: 864) HUMTEN_PEA_1_T18 4879 5040 (SEQ ID NO: 865) HUMTEN_PEA_1_T19 4606 4767 (SEQ ID NO: 866)

Segment cluster HUMTEN_PEA_(—)1_node_(—)79 (SEQ ID NO: 906) according to the present invention is supported by 139 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HUMTEN_PEA_(—)1_T4 (SEQ ID NO: 857), HUMTEN_PEA_(—)1_T5 (SEQ ID NO: 858), HUMTEN_PEA_(—)1_T6 (SEQ ID NO: 859), HUMTEN_PEA_(—)1_T7 (SEQ ID NO: 860), HUMTEN_PEA_(—)1_T11 (SEQ ID NO: 861), HUMTEN_PEA_(—)1_T14 (SEQ ID NO: 862), HUMTEN_PEA_(—)1_T16 (SEQ ID NO: 863), HUMTEN_PEA_(—)1_T17 (SEQ ID NO: 864), HUMTEN_PEA_(—)1_T18 (SEQ ID NO: 865), HUMTEN_PEA_(—)1_T19 (SEQ ID NO: 866) and HUMTEN_PEA_(—)1_T20 (SEQ ID NO. 867). Table 94 below describes the starting and ending position of this segment on each transcript.

TABLE 94 Segment location on transcripts Segment Segment Transcript name starting position ending position HUMTEN_PEA_1_T4 6955 7118 (SEQ ID NO: 857) HUMTEN_PEA_1_T5 11162 11325 (SEQ ID NO: 858) HUMTEN_PEA_1_T6 8256 8419 (SEQ ID NO: 859) HUMTEN_PEA_1_T7 6406 6569 (SEQ ID NO: 860) HUMTEN_PEA_1_T11 6406 6569 (SEQ ID NO: 861) HUMTEN_PEA_1_T14 5860 6023 (SEQ ID NO: 862) HUMTEN_PEA_1_T16 5587 5750 (SEQ ID NO: 863) HUMTEN_PEA_1_T17 7476 7639 (SEQ ID NO: 864) HUMTEN_PEA_1_T18 5041 5204 (SEQ ID NO: 865) HUMTEN_PEA_1_T19 4768 4931 (SEQ ID NO: 866) HUMTEN_PEA_1_T20 6420 6583 (SEQ ID NO. 867)

Segment cluster HUMTEN_PEA_(—)1_node_(—)83 (SEQ ID NO: 907) according to the present invention is supported by 150 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HUMTEN_PEA_(—)1_T4 (SEQ ID NO: 857), HUMTEN_PEA_(—)1_T5 (SEQ ID NO: 858), HUMTEN_PEA_(—)1_T6 (SEQ ID NO: 859), HUMTEN_PEA_(—)1_T7 (SEQ ID NO: 860), HUMTEN_PEA_(—)1_T11 (SEQ ID NO: 861), HUMTEN_PEA_(—)1_T14 (SEQ ID NO: 862), HUMTEN_PEA_(—)1_T16 (SEQ ID NO: 863), HUMTEN_PEA_(—)1_T17 (SEQ ID NO: 864), HUMTEN_PEA_(—)1_T18 (SEQ ID NO: 865), HUMTEN_PEA_(—)1_T19 (SEQ ID NO: 866) and HUMTEN_PEA_(—)1_T20 (SEQ ID NO. 867). Table 95 below describes the starting and ending position of this segment on each transcript.

TABLE 95 Segment location on transcripts Segment Segment Transcript name starting position ending position HUMTEN_PEA_1_T4 7119 7240 (SEQ ID NO: 857) HUMTEN_PEA_1_T5 11326 11447 (SEQ ID NO: 858) HUMTEN_PEA_1_T6 8420 8541 (SEQ ID NO: 859) HUMTEN_PEA_1_T7 6570 6691 (SEQ ID NO: 860) HUMTEN_PEA_1_T11 6570 6691 (SEQ ID NO: 861) HUMTEN_PEA_1_T14 6024 6145 (SEQ ID NO: 862) HUMTEN_PEA_1_T16 5751 5872 (SEQ ID NO: 863) HUMTEN_PEA_1_T17 7640 7761 (SEQ ID NO: 864) HUMTEN_PEA_1_T18 5205 5326 (SEQ ID NO: 865) HUMTEN_PEA_1_T19 4932 5053 (SEQ ID NO: 866) HUMTEN_PEA_1_T20 6584 6705 (SEQ ID NO. 867)

Segment cluster HUMTEN_PEA_(—)1_node_(—)89 (SEQ ID NO: 908) according to the present invention is supported by 196 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HUMTEN_PEA_(—)1_T4 (SEQ ID NO: 857), HUMTEN_PEA_(—)1_T5 (SEQ ID NO: 858), HUMTEN_PEA_(—)1_T6 (SEQ ID NO: 859), HUMTEN_PEA_(—)1_T7 (SEQ ID NO: 860), HUMTEN_PEA_(—)1_T11 (SEQ ID NO: 861), HUMTEN_PEA_(—)1_T14 (SEQ ID NO: 862), HUMTEN_PEA_(—)1_T16 (SEQ ID NO: 863), HUMTEN_PEA_(—)1_T17 (SEQ ID NO: 864), HUMTEN_PEA_(—)1_T18 (SEQ ID NO: 865), HUMTEN_PEA_(—)1_T19 (SEQ ID NO: 866) and HUMTEN_PEA_(—)1_T20 (SEQ ID NO. 867). Table 96 below describes the starting and ending position of this segment on each transcript.

TABLE 96 Segment location on transcripts Segment Segment Transcript name starting position ending position HUMTEN_PEA_1_T4 7559 8816 (SEQ ID NO: 857) HUMTEN_PEA_1_T5 11766 13023 (SEQ ID NO: 858) HUMTEN_PEA_1_T6 8860 10117 (SEQ ID NO: 859) HUMTEN_PEA_1_T7 7010 8267 (SEQ ID NO: 860) HUMTEN_PEA_1_T11 7010 8267 (SEQ ID NO: 861) HUMTEN_PEA_1_T14 6464 7721 (SEQ ID NO: 862) HUMTEN_PEA_1_T16 6191 7448 (SEQ ID NO: 863) HUMTEN_PEA_1_T17 8080 9337 (SEQ ID NO: 864) HUMTEN_PEA_1_T18 5645 6902 (SEQ ID NO: 865) HUMTEN_PEA_1_T19 5372 6629 (SEQ ID NO: 866) HUMTEN_PEA_1_T20 7024 8281 (SEQ ID NO. 867)

According to an optional embodiment of the present invention, short segments related to the above cluster are also provided. These segments are up to about 120 bp in length, and so are included in a separate description.

Segment cluster HUMTEN_PEA_(—)1_node_(—)7 (SEQ ID NO: 909) according to the present invention is supported by 25 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HUMTEN_PEA_(—)1_T4 (SEQ ID NO: 857), HUMTEN_PEA_(—)1_T5 (SEQ ID NO: 858), HUMTEN_PEA_(—)1_T6 (SEQ ID NO: 859), HUMTEN_PEA_(—)1_T7 (SEQ ID NO: 860), HUMTEN_PEA_(—)1_T11 (SEQ ID NO: 861), HUMTEN_PEA_(—)1_T14 (SEQ ID NO: 862), HUMTEN_PEA_(—)1_T16 (SEQ ID NO: 863), HUMTEN_PEA_(—)1_T17 (SEQ ID NO: 864), HUMTEN_PEA_(—)1_T18 (SEQ ID NO: 865), HUMTEN_PEA_(—)1_T19 (SEQ ID NO: 866), HUMTEN_PEA_(—)1_T20 (SEQ ID NO. 867), HUMTEN_PEA_(—)1_T23 (SEQ ID NO: 868), HUMTEN_PEA_(—)1_T32 (SEQ ID NO: 869), HUMTEN_PEA_(—)1_T35 (SEQ ID NO: 870), HUMTEN_PEA_(—)1_T36 (SEQ ID NO: 871), HUMTEN_PEA_(—)1_T37 (SEQ ID NO: 872), HUMTEN_PEA_(—)1_T39 (SEQ ID NO: 873), HUMTEN_PEA_(—)1_T40 (SEQ ID NO: 874) and HUMTEN_PEA_(—)1_T41 (SEQ ID NO: 875). Table 97 below describes the starting and ending position of this segment on each transcript.

TABLE 97 Segment location on transcripts Segment Segment Transcript name starting position ending position HUMTEN_PEA_1_T4 1926 2040 (SEQ ID NO: 857) HUMTEN_PEA_1_T5 1926 2040 (SEQ ID NO: 858) HUMTEN_PEA_1_T6 1926 2040 (SEQ ID NO: 859) HUMTEN_PEA_1_T7 1926 2040 (SEQ ID NO: 860) HUMTEN_PEA_1_T11 1926 2040 (SEQ ID NO: 861) HUMTEN_PEA_1_T14 1926 2040 (SEQ ID NO: 862) HUMTEN_PEA_1_T16 1926 2040 (SEQ ID NO: 863) HUMTEN_PEA_1_T17 1926 2040 (SEQ ID NO: 864) HUMTEN_PEA_1_T18 1926 2040 (SEQ ID NO: 865) HUMTEN_PEA_1_T19 1926 2040 (SEQ ID NO: 866) HUMTEN_PEA_1_T20 1926 2040 (SEQ ID NO. 867) HUMTEN_PEA_1_T23 1926 2040 (SEQ ID NO: 868) HUMTEN_PEA_1_T32 1926 2040 (SEQ ID NO: 869) HUMTEN_PEA_1_T35 1926 2040 (SEQ ID NO: 870) HUMTEN_PEA_1_T36 1926 2040 (SEQ ID NO: 871) HUMTEN_PEA_1_T37 1926 2040 (SEQ ID NO: 872) HUMTEN_PEA_1_T39 1926 2040 (SEQ ID NO: 873) HUMTEN_PEA_1_T40 1926 2040 (SEQ ID NO: 874) HUMTEN_PEA_1_T41 1926 2040 (SEQ ID NO: 875)

Segment cluster HUMTEN_PEA_(—)1_node_(—)8 according to the present invention is supported by 25 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HUMTEN_PEA_(—)1_T4 (SEQ ID NO: 857), HUMTEN_PEA_(—)1_T5 (SEQ ID NO: 858), HUMTEN_PEA_(—)1_T6 (SEQ ID NO: 859), HUMTEN_PEA_(—)1_T7 (SEQ ID NO: 860), HUMTEN_PEA_(—)1_T11 (SEQ ID NO: 861), HUMTEN_PEA_(—)1_T14 (SEQ ID NO: 862), HUMTEN_PEA_(—)1_T16 (SEQ ID NO: 863), HUMTEN_PEA_(—)1_T17 (SEQ ID NO: 864), HUMTEN_PEA_(—)1_T18 (SEQ ID NO: 865), HUMTEN_PEA_(—)1_T19 (SEQ ID NO: 866), HUMTEN_PEA_(—)1_T20 (SEQ ID NO. 867), HUMTEN_PEA_(—)1_T23 (SEQ ID NO: 868), HUMTEN_PEA_(—)1_T32 (SEQ ID NO: 869), HUMTEN_PEA_(—)1_T35 (SEQ ID NO: 870), HUMTEN_PEA_(—)1_T36 (SEQ ID NO: 871), HUMTEN_PEA_(—)1_T37 (SEQ ID NO: 872), HUMTEN_PEA_(—)1_T39 (SEQ ID NO: 873), HUMTEN_PEA_(—)1_T40 (SEQ ID NO: 874) and HUMTEN_PEA_(—)1_T41 (SEQ ID NO: 875). Table 98 below describes the starting and ending position of this segment on each transcript.

TABLE 98 Segment location on transcripts Segment Segment Transcript name starting position ending position HUMTEN_PEA_1_T4 2041 2134 (SEQ ID NO: 857) HUMTEN_PEA_1_T5 2041 2134 (SEQ ID NO: 858) HUMTEN_PEA_1_T6 2041 2134 (SEQ ID NO: 859) HUMTEN_PEA_1_T7 2041 2134 (SEQ ID NO: 860) HUMTEN_PEA_1_T11 2041 2134 (SEQ ID NO: 861) HUMTEN_PEA_1_T14 2041 2134 (SEQ ID NO: 862) HUMTEN_PEA_1_T16 2041 2134 (SEQ ID NO: 863) HUMTEN_PEA_1_T17 2041 2134 (SEQ ID NO: 864) HUMTEN_PEA_1_T18 2041 2134 (SEQ ID NO: 865) HUMTEN_PEA_1_T19 2041 2134 (SEQ ID NO: 866) HUMTEN_PEA_1_T20 2041 2134 (SEQ ID NO. 867) HUMTEN_PEA_1_T23 2041 2134 (SEQ ID NO: 868) HUMTEN_PEA_1_T32 2041 2134 (SEQ ID NO: 869) HUMTEN_PEA_1_T35 2041 2134 (SEQ ID NO: 870) HUMTEN_PEA_1_T36 2041 2134 (SEQ ID NO: 871) HUMTEN_PEA_1_T37 2041 2134 (SEQ ID NO: 872) HUMTEN_PEA_1_T39 2041 2134 (SEQ ID NO: 873) HUMTEN_PEA_1_T40 2041 2134 (SEQ ID NO: 874) HUMTEN_PEA_1_T41 2041 2134 (SEQ ID NO: 875)

Segment cluster HUMTEN_PEA_(—)1_node_(—)9 (SEQ ID NO: 911) according to the present invention is supported by 25 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HUMTEN_PEA_(—)1_T4 (SEQ ID NO: 857), HUMTEN_PEA_(—)1_T5 (SEQ ID NO: 858), HUMTEN_PEA_(—)1_T6 (SEQ ID NO: 859), HUMTEN_PEA_(—)1_T7 (SEQ ID NO: 860), HUMTEN_PEA_(—)1_T11 (SEQ ID NO: 861), HUMTEN_PEA_(—)1_T14 (SEQ ID NO: 862), HUMTEN_PEA_(—)1_T16 (SEQ ID NO: 863), HUMTEN_PEA_(—)1_T17 (SEQ ID NO: 864), HUMTEN_PEA_(—)1_T18 (SEQ ID NO: 865), HUMTEN_PEA_(—)1_T19 (SEQ ID NO: 866), HUMTEN_PEA_(—)1_T20 (SEQ ID NO. 867), HUMTEN_PEA_(—)1_T23 (SEQ ID NO: 868), HUMTEN_PEA_(—)1_T32 (SEQ ID NO: 869), HUMTEN_PEA_(—)1_T35 (SEQ ID NO: 870), HUMTEN_PEA_(—)1_T36 (SEQ ID NO: 871), HUMTEN_PEA_(—)1_T37 (SEQ ID NO: 872), HUMTEN_PEA_(—)1_T39 (SEQ ID NO: 873), HUMTEN_PEA_(—)1_T40 (SEQ ID NO: 874) and HUMTEN_PEA_(—)1_T41 (SEQ ID NO: 875). Table 99 below describes the starting and ending position of this segment on each transcript.

TABLE 99 Segment location on transcripts Segment Segment Transcript name starting position ending position HUMTEN_PEA_1_T4 2135 2214 (SEQ ID NO: 857) HUMTEN_PEA_1_T5 2135 2214 (SEQ ID NO: 858) HUMTEN_PEA_1_T6 2135 2214 (SEQ ID NO: 859) HUMTEN_PEA_1_T7 2135 2214 (SEQ ID NO: 860) HUMTEN_PEA_1_T11 2135 2214 (SEQ ID NO: 861) HUMTEN_PEA_1_T14 2135 2214 (SEQ ID NO: 862) HUMTEN_PEA_1_T16 2135 2214 (SEQ ID NO: 863) HUMTEN_PEA_1_T17 2135 2214 (SEQ ID NO: 864) HUMTEN_PEA_1_T18 2135 2214 (SEQ ID NO: 865) HUMTEN_PEA_1_T19 2135 2214 (SEQ ID NO: 866) HUMTEN_PEA_1_T20 2135 2214 (SEQ ID NO. 867) HUMTEN_PEA_1_T23 2135 2214 (SEQ ID NO: 868) HUMTEN_PEA_1_T32 2135 2214 (SEQ ID NO: 869) HUMTEN_PEA_1_T35 2135 2214 (SEQ ID NO: 870) HUMTEN_PEA_1_T36 2135 2214 (SEQ ID NO: 871) HUMTEN_PEA_1_T37 2135 2214 (SEQ ID NO: 872) HUMTEN_PEA_1_T39 2135 2214 (SEQ ID NO: 873) HUMTEN_PEA_1_T40 2135 2214 (SEQ ID NO: 874) HUMTEN_PEA_1_T41 2135 2214 (SEQ ID NO: 875)

Segment cluster HUMTEN_PEA_(—)1_node_(—)14 (SEQ ID NO: 912) according to the present invention is supported by 24 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HUMTEN_PEA_(—)1_T4 (SEQ ID NO: 857), HUMTEN_PEA_(—)1_T5 (SEQ ID NO: 858), HUMTEN_PEA_(—)1_T6 (SEQ ID NO: 859), HUMTEN_PEA_(—)1_T7 (SEQ ID NO: 860), HUMTEN_PEA_(—)1_T11 (SEQ ID NO: 861), HUMTEN_PEA_(—)1_T14 (SEQ ID NO: 862), HUMTEN_PEA_(—)1_T16 (SEQ ID NO: 863), HUMTEN_PEA_(—)1_T17 (SEQ ID NO: 864), HUMTEN_PEA_(—)1_T18 (SEQ ID NO: 865), HUMTEN_PEA_(—)1_T19 (SEQ ID NO: 866), HUMTEN_PEA_(—)1_T20 (SEQ ID NO. 867), HUMTEN_PEA_(—)1_T23 (SEQ ID NO: 868), HUMTEN_PEA_(—)1_T32 (SEQ ID NO: 869), HUMTEN_PEA_(—)1_T35 (SEQ ID NO: 870), HUMTEN_PEA_(—)1_T36 (SEQ ID NO: 871), HUMTEN_PEA_(—)1_T37 (SEQ ID NO: 872), HUMTEN_PEA_(—)1_T39 (SEQ ID NO: 873) and HUMTEN_PEA_(—)1_T40 (SEQ ID NO: 874). Table 100 below describes the starting and ending position of this segment on each transcript.

TABLE 100 Segment location on transcripts Segment Segment Transcript name starting position ending position HUMTEN_PEA_1_T4 2479 2594 (SEQ ID NO: 857) HUMTEN_PEA_1_T5 2479 2594 (SEQ ID NO: 858) HUMTEN_PEA_1_T6 2479 2594 (SEQ ID NO: 859) HUMTEN_PEA_1_T7 2479 2594 (SEQ ID NO: 860) HUMTEN_PEA_1_T11 2479 2594 (SEQ ID NO: 861) HUMTEN_PEA_1_T14 2479 2594 (SEQ ID NO: 862) HUMTEN_PEA_1_T16 2479 2594 (SEQ ID NO: 863) HUMTEN_PEA_1_T17 2479 2594 (SEQ ID NO: 864) HUMTEN_PEA_1_T18 2479 2594 (SEQ ID NO: 865) HUMTEN_PEA_1_T19 2479 2594 (SEQ ID NO: 866) HUMTEN_PEA_1_T20 2479 2594 (SEQ ID NO. 867) HUMTEN_PEA_1_T23 2479 2594 (SEQ ID NO: 868) HUMTEN_PEA_1_T32 2479 2594 (SEQ ID NO: 869) HUMTEN_PEA_1_T35 2479 2594 (SEQ ID NO: 870) HUMTEN_PEA_1_T36 2479 2594 (SEQ ID NO: 871) HUMTEN_PEA_1_T37 2479 2594 (SEQ ID NO: 872) HUMTEN_PEA_1_T39 2479 2594 (SEQ ID NO: 873) HUMTEN_PEA_1_T40 2479 2594 (SEQ ID NO: 874)

Segment cluster HUMTEN_PEA_(—)1_node_(—)17 (SEQ ID NO: 913) according to the present invention is supported by 1 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HUMTEN_PEA_(—)1_T40 (SEQ ID NO: 874). Table 101 below describes the starting and ending position of this segment on each transcript.

TABLE 101 Segment location on transcripts Segment Segment Transcript name starting position ending position HUMTEN_PEA_1_T40 2752 2817 (SEQ ID NO: 874)

Segment cluster HUMTEN_PEA_(—)1_node_(—)21 (SEQ ID NO: 914) according to the present invention is supported by 24 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HUMTEN_PEA_(—)1_T4 (SEQ ID NO: 857), HUMTEN_PEA_(—)1_T5 (SEQ ID NO: 858), HUMTEN_PEA_(—)1_T6 (SEQ ID NO: 859), HUMTEN_PEA_(—)1_T7 (SEQ ID NO: 860), HUMTEN_PEA_(—)1_T11 (SEQ ID NO: 861), HUMTEN_PEA_(—)1_T14 (SEQ ID NO: 862), HUMTEN_PEA_(—)1_T16 (SEQ ID NO: 863), HUMTEN_PEA_(—)1_T17 (SEQ ID NO: 864), HUMTEN_PEA_(—)1_T18 (SEQ ID NO: 865), HUMTEN_PEA_(—)1_T19 (SEQ ID NO: 866), HUMTEN_PEA_(—)1_T20 (SEQ ID NO. 867), HUMTEN_PEA_(—)1_T23 (SEQ ID NO: 868), HUMTEN_PEA_(—)1_T32 (SEQ ID NO: 869), HUMTEN_PEA_(—)1_T35 (SEQ ID NO: 870), HUMTEN_PEA_(—)1_T36 (SEQ ID NO: 871), HUMTEN_PEA_(—)1_T37 (SEQ ID NO: 872) and HUMTEN_PEA_(—)1_T39 (SEQ ID NO: 873). Table 102 below describes the starting and ending position of this segment on each transcript.

TABLE 102 Segment location on transcripts Segment Segment Transcript name starting position ending position HUMTEN_PEA_1_T4 3022 3111 (SEQ ID NO: 857) HUMTEN_PEA_1_T5 3022 3111 (SEQ ID NO: 858) HUMTEN_PEA_1_T6 3022 3111 (SEQ ID NO: 859) HUMTEN_PEA_1_T7 3022 3111 (SEQ ID NO: 860) HUMTEN_PEA_1_T11 3022 3111 (SEQ ID NO: 861) HUMTEN_PEA_1_T14 3022 3111 (SEQ ID NO: 862) HUMTEN_PEA_1_T16 3022 3111 (SEQ ID NO: 863) HUMTEN_PEA_1_T17 3022 3111 (SEQ ID NO: 864) HUMTEN_PEA_1_T18 3022 3111 (SEQ ID NO: 865) HUMTEN_PEA_1_T19 3022 3111 (SEQ ID NO: 866) HUMTEN_PEA_1_T20 3022 3111 (SEQ ID NO. 867) HUMTEN_PEA_1_T23 3022 3111 (SEQ ID NO: 868) HUMTEN_PEA_1_T32 3022 3111 (SEQ ID NO: 869) HUMTEN_PEA_1_T35 3022 3111 (SEQ ID NO: 870) HUMTEN_PEA_1_T36 3022 3111 (SEQ ID NO: 871) HUMTEN_PEA_1_T37 3022 3111 (SEQ ID NO: 872) HUMTEN_PEA_1_T39 3022 3111 (SEQ ID NO: 873)

Segment cluster HUMTEN_PEA_(—)1_node_(—)22 (SEQ ID NO: 915) according to the present invention is supported by 27 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HUMTEN_PEA_(—)1_T4 (SEQ ID NO: 857), HUMTEN_PEA_(—)1_T5 (SEQ ID NO: 858), HUMTEN_PEA_(—)1_T6 (SEQ ID NO: 859), HUMTEN_PEA_(—)1_T7 (SEQ ID NO: 860), HUMTEN_PEA_(—)1_T11 (SEQ ID NO: 861), HUMTEN_PEA_(—)1_T14 (SEQ ID NO: 862), HUMTEN_PEA_(—)1_T16 (SEQ ID NO: 863), HUMTEN_PEA_(—)1_T17 (SEQ ID NO: 864), HUMTEN_PEA_(—)1_T18 (SEQ ID NO: 865), HUMTEN_PEA_(—)1_T19 (SEQ ID NO: 866), HUMTEN_PEA_(—)1_T20 (SEQ ID NO. 867), HUMTEN_PEA_(—)1_T23 (SEQ ID NO: 868), HUMTEN_PEA_(—)1_T32 (SEQ ID NO: 869), HUMTEN_PEA_(—)1_T35 (SEQ ID NO: 870), HUMTEN_PEA_(—)1_T36 (SEQ ID NO: 871), HUMTEN_PEA_(—)1_T37 (SEQ ID NO: 872) and HUMTEN_PEA_(—)1_T39 (SEQ ID NO: 873). Table 103 below describes the starting and ending position of this segment on each transcript.

TABLE 103 Segment location on transcripts Segment Segment Transcript name starting position ending position HUMTEN_PEA_1_T4 3112 3207 (SEQ ID NO: 857) HUMTEN_PEA_1_T5 3112 3207 (SEQ ID NO: 858) HUMTEN_PEA_1_T6 3112 3207 (SEQ ID NO: 859) HUMTEN_PEA_1_T7 3112 3207 (SEQ ID NO: 860) HUMTEN_PEA_1_T11 3112 3207 (SEQ ID NO: 861) HUMTEN_PEA_1_T14 3112 3207 (SEQ ID NO: 862) HUMTEN_PEA_1_T16 3112 3207 (SEQ ID NO: 863) HUMTEN_PEA_1_T17 3112 3207 (SEQ ID NO: 864) HUMTEN_PEA_1_T18 3112 3207 (SEQ ID NO: 865) HUMTEN_PEA_1_T19 3112 3207 (SEQ ID NO: 866) HUMTEN_PEA_1_T20 3112 3207 (SEQ ID NO. 867) HUMTEN_PEA_1_T23 3112 3207 (SEQ ID NO: 868) HUMTEN_PEA_1_T32 3112 3207 (SEQ ID NO: 869) HUMTEN_PEA_1_T35 3112 3207 (SEQ ID NO: 870) HUMTEN_PEA_1_T36 3112 3207 (SEQ ID NO: 871) HUMTEN_PEA_1_T37 3112 3207 (SEQ ID NO: 872) HUMTEN_PEA_1_T39 3112 3207 (SEQ ID NO: 873)

Segment cluster HUMTEN_PEA_(—)1_node_(—)25 (SEQ ID NO: 916) according to the present invention is supported by 31 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HUMTEN_PEA_(—)1_T4 (SEQ ID NO: 857), HUMTEN_PEA_(—)1_T5 (SEQ ID NO: 858), HUMTEN_PEA_(—)1_T6 (SEQ ID NO: 859), HUMTEN_PEA_(—)1_T7 (SEQ ID NO: 860), HUMTEN_PEA_(—)1_T11 (SEQ ID NO: 861), HUMTEN_PEA_(—)1_T14 (SEQ ID NO: 862), HUMTEN_PEA_(—)1_T16 (SEQ ID NO: 863), HUMTEN_PEA_(—)1_T17 (SEQ ID NO: 864), HUMTEN_PEA_(—)1_T18 (SEQ ID NO: 865), HUMTEN_PEA_(—)1_T19 (SEQ ID NO: 866), HUMTEN_PEA_(—)1_T20 (SEQ ID NO. 867), HUMTEN_PEA_(—)1_T23 (SEQ ID NO: 868), HUMTEN_PEA_(—)1_T32 (SEQ ID NO: 869), HUMTEN_PEA_(—)1_T35 (SEQ ID NO: 870), HUMTEN_PEA_(—)1_T36 (SEQ ID NO: 871) and HUMTEN_PEA_(—)1_T37 (SEQ ID NO: 872). Table 104 below describes the starting and ending position of this segment on each transcript.

TABLE 104 Segment location on transcripts Segment Segment Transcript name starting position ending position HUMTEN_PEA_1_T4 3208 3297 (SEQ ID NO: 857) HUMTEN_PEA_1_T5 3208 3297 (SEQ ID NO: 858) HUMTEN_PEA_1_T6 3208 3297 (SEQ ID NO: 859) HUMTEN_PEA_1_T7 3208 3297 (SEQ ID NO: 860) HUMTEN_PEA_1_T11 3208 3297 (SEQ ID NO: 861) HUMTEN_PEA_1_T14 3208 3297 (SEQ ID NO: 862) HUMTEN_PEA_1_T16 3208 3297 (SEQ ID NO: 863) HUMTEN_PEA_1_T17 3208 3297 (SEQ ID NO: 864) HUMTEN_PEA_1_T18 3208 3297 (SEQ ID NO: 865) HUMTEN_PEA_1_T19 3208 3297 (SEQ ID NO: 866) HUMTEN_PEA_I_T20 3208 3297 (SEQ ID NO. 867) HUMTEN_PEA_1_T23 3208 3297 (SEQ ID NO: 868) HUMTEN_PEA_1_T32 3208 3297 (SEQ ID NO: 869) HUMTEN_PEA_1_T35 3208 3297 (SEQ ID NO: 870) HUMTEN_PEA_1_T36 3208 3297 (SEQ ID NO: 871) HUMTEN_PEA_1_T37 3208 3297 (SEQ ID NO: 872)

Segment cluster HUMTEN_PEA_(—)1_node_(—)36 (SEQ ID NO: 917) according to the present invention is supported by 2 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HUMTEN_PEA_(—)1_T35 (SEQ ID NO: 870). Table 105 below describes the starting and ending position of this segment on each transcript.

TABLE 105 Segment location on transcripts Segment Segment Transcript name starting position ending position HUMTEN_PEA_1_T35 4381 4446 (SEQ ID NO: 870)

Segment cluster HUMTEN_PEA_(—)1_node_(—)53 (SEQ ID NO: 918) according to the present invention is supported by 68 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HUMTEN_PEA_(—)1_T4 (SEQ ID NO: 857), HUMTEN_PEA_(—)1_T5 (SEQ ID NO: 858), HUMTEN_PEA_(—)1_T6 (SEQ ID NO: 859), HUMTEN_PEA_(—)1_T7 (SEQ ID NO: 860), HUMTEN_PEA_(—)1_T11 (SEQ ID NO: 861), HUMTEN_PEA_(—)1_T14 (SEQ ID NO: 862), HUMTEN_PEA_(—)1_T16 (SEQ ID NO: 863), HUMTEN_PEA_(—)1_T17 (SEQ ID NO: 864), HUMTEN_PEA_(—)1_T18 (SEQ ID NO: 865), HUMTEN_PEA_(—)1_T19 (SEQ ID NO: 866), HUMTEN_PEA_(—)1_T20 (SEQ ID NO. 867) and HUMTEN_PEA_(—)1_T23 (SEQ ID NO: 868). Table 106 below describes the starting and ending position of this segment on each transcript.

TABLE 106 Segment location on transcripts Segment Segment Transcript name starting position ending position HUMTEN_PEA_1_T4 6016 6050 (SEQ ID NO: 857) HUMTEN_PEA_1_T5 10223 10257 (SEQ ID NO: 858) HUMTEN_PEA_1_T6 7317 7351 (SEQ ID NO: 859) HUMTEN_PEA_1_T7 5467 5501 (SEQ ID NO: 860) HUMTEN_PEA_1_T11 5467 5501 (SEQ ID NO: 861) HUMTEN_PEA_1_T14 4921 4955 (SEQ ID NO: 862) HUMTEN_PEA_1_T16 4648 4682 (SEQ ID NO: 863) HUMTEN_PEA_1_T17 5740 5774 (SEQ ID NO: 864) HUMTEN_PEA_1_T18 4102 4136 (SEQ ID NO: 865) HUMTEN_PEA_1_T19 3829 3863 (SEQ ID NO: 866) HUMTEN_PEA_1_T20 5740 5774 (SEQ ID NO. 867) HUMTEN_PEA_1_T23 5740 5774 (SEQ ID NO: 868)

Segment cluster HUMTEN_PEA_(—)1_node_(—)54 (SEQ ID NO: 919)) according to the present invention is supported by 72 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HUMTEN_PEA_(—)1_T4 (SEQ ID NO: 857), HUMTEN_PEA_(—)1_T5 (SEQ ID NO: 858), HUMTEN_PEA_(—)1_T6 (SEQ ID NO: 859), HUMTEN_PEA_(—)1_T7 (SEQ ID NO: 860), HUMTEN_PEA_(—)1_T11 (SEQ ID NO: 861), HUMTEN_PEA_(—)1_T14 (SEQ ID NO: 862), HUMTEN_PEA_(—)1_T16 (SEQ ID NO: 863), HUMTEN_PEA_(—)1_T17 (SEQ ID NO: 864), HUMTEN_PEA_(—)1_T18 (SEQ ID NO: 865), HUMTEN_PEA_(—)1_T19 (SEQ ID NO: 866), HUMTEN_PEA_(—)1_T20 (SEQ ID NO. 867) and HUMTEN_PEA_(—)1_T23 (SEQ ID NO: 868). Table 107 below describes the starting and ending position of this segment on each transcript.

TABLE 107 Segment location on transcripts Segment Segment Transcript starting ending name position position HUMTEN_PEA_1_T4 6051 6135 (SEQ ID NO: 857) HUMTEN_PEA_1_T5 10258 10342 (SEQ ID NO: 858) HUMTEN_PEA_1_T6 7352 7436 (SEQ ID NO: 859) HUMTEN_PEA_1_T7 5502 5586 (SEQ ID NO: 860) HUMTEN_PEA_1_T11 5502 5586 (SEQ ID NO: 861) HUMTEN_PEA_1_T14 4956 5040 (SEQ ID NO: 862) HUMTEN_PEA_1_T16 4683 4767 (SEQ ID NO: 863) HUMTEN_PEA_1_T17 5775 5859 (SEQ ID NO: 864) HUMTEN_PEA_1_T18 4137 4221 (SEQ ID NO: 865) HUMTEN_PEA_1_T19 3864 3948 (SEQ ID NO: 866) HUMTEN_PEA_1_T20 5775 5859 (SEQ ID NO. 867) HUMTEN_PEA_1_T23 5775 5859 (SEQ ID NO: 868)

Segment cluster HUMTEN_PEA_(—)1_node_(—)57 (SEQ ID NO: 920) according to the present invention can be found in the following transcript(s): HUMTEN_PEA_(—)1_T4 (SEQ ID NO: 857), HUMTEN_PEA_(—)1_T5 (SEQ ID NO: 858), HUMTEN_PEA_(—)1_T6 (SEQ ID NO: 859), HUMTEN_PEA_(—)1_T7 (SEQ ID NO: 860), HUMTEN_PEA_(—)1_T11 (SEQ ID NO: 861), HUMTEN_PEA_(—)1_T14 (SEQ ID NO: 862), HUMTEN_PEA_(—)1_(—T)16 (SEQ ID NO: 863), HUMTEN_PEA_(—)1_T17 (SEQ ID NO: 864), HUMTEN_PEA_(—)1_T18 (SEQ ID NO: 865), HUMTEN_PEA_(—)1_T19 (SEQ ID NO: 866), HUMTEN_PEA_(—)1_T20 (SEQ ID NO. 867) and HUMTEN_PEA_(—)1_T23 (SEQ ID NO: 868). Table 108 below describes the starting and ending position of this segment on each transcript.

TABLE 108 Segment location on transcripts Segment Segment Transcript starting ending name position position HUMTEN_PEA_1_T4 6262 6279 (SEQ ID NO: 857) HUMTEN_PEA_1_T5 10469 10486 (SEQ ID NO: 858) HUMTEN_PEA_1_T6 7563 7580 (SEQ ID NO: 859) HUMTEN_PEA_1_T7 5713 5730 (SEQ ID NO: 860) HUMTEN_PEA_1_T11 5713 5730 (SEQ ID NO: 861) HUMTEN_PEA_1_T14 5167 5184 (SEQ ID NO: 862) HUMTEN_PEA_1_T16 4894 4911 (SEQ ID NO: 863) HUMTEN_PEA_1_T17 5986 6003 (SEQ ID NO: 864) HUMTEN_PEA_1_T18 4348 4365 (SEQ ID NO: 865) HUMTEN_PEA_1_T19 4075 4092 (SEQ ID NO: 866) HUMTEN_PEA_1_T20 5986 6003 (SEQ ID NO. 867) HUMTEN_PEA_1_T23 5986 6003 (SEQ ID NO: 868)

Segment cluster HUMTEN_PEA_(—)1_node_(—)61 (SEQ ID NO: 921) according to the present invention is supported by 75 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HUMTEN_PEA_(—)1_T4 (SEQ ID NO: 857), HUMTEN_PEA_(—)1_T5 (SEQ ID NO: 858), HUMTEN_PEA_(—)1_T6 (SEQ ID NO: 859), HUMTEN_PEA_(—)1_T7 (SEQ ID NO: 860), HUMTEN_PEA_(—)1_T11 (SEQ ID NO: 861), HUMTEN_PEA_(—)1_T14 (SEQ ID NO: 862), HUMTEN_PEA_(—)1_T16 (SEQ ID NO: 863), HUMTEN_PEA_(—)1_T7 (SEQ ID NO: 864), HUMTEN_PEA_(—)1_T18 (SEQ ID NO: 865), HUMTEN_PEA_(—)1_T19 (SEQ ID NO: 866), HUMTEN_PEA_(—)1_T20 (SEQ ID NO. 867) and HUMTEN_PEA_(—)1_T23 (SEQ ID NO: 868). Table 109 below describes the starting and ending position of this segment on each transcript.

TABLE 109 Segment location on transcripts Segment Segment Transcript starting ending name position position HUMTEN_PEA_1_T4 6280 6363 (SEQ ID NO: 857) HUMTEN_PEA_1_T5 10487 10570 (SEQ ID NO: 858) HUMTEN_PEA_1_T6 7581 7664 (SEQ ID NO: 859) HUMTEN_PEA_1_T7 5731 5814 (SEQ ID NO: 860) HUMTEN_PEA_1_T11 5731 5814 (SEQ ID NO: 861) HUMTEN_PEA_1_T14 5185 5268 (SEQ ID NO: 862) HUMTEN_PEA_1_T16 4912 4995 (SEQ ID NO: 863) HUMTEN_PEA_1_T17 6004 6087 (SEQ ID NO: 864) HUMTEN_PEA_1_T18 4366 4449 (SEQ ID NO: 865) HUMTEN_PEA_1_T19 4093 4176 (SEQ ID NO: 866) HUMTEN_PEA_1_T20 6004 6087 (SEQ ID NO. 867) HUMTEN_PEA_1_T23 6004 6087 (SEQ ID NO: 868)

Segment cluster HUMTEN_PEA_(—)1_node_(—)62 (SEQ ID NO: 922) according to the present invention is supported by 75 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HUMTEN_PEA_(—)1_T4 (SEQ ID NO: 857), HUMTEN_PEA_(—)1_T5 (SEQ ID NO: 858), HUMTEN_PEA_(—)1_T6 (SEQ ID NO: 859), HUMTEN_PEA_(—)1_T7 (SEQ ID NO: 860), HUMTEN_PEA_(—)1_T11 (SEQ ID NO: 861), HUMTEN_PEA_(—)1_T14 (SEQ ID NO: 862), HUMTEN_PEA_(—)1_T16 (SEQ ID NO: 863), HUMTEN_PEA_(—)1_T17 (SEQ ID NO: 864), HUMTEN_PEA_(—)1_T18 (SEQ ID NO: 865), HUMTEN_PEA_(—)1_T19 (SEQ ID NO: 866), HUMTEN_PEA_(—)1_T20 (SEQ ID NO. 867) and HUMTEN_PEA_(—)1_T23 (SEQ ID NO: 868). Table 110 below describes the starting and ending position of this segment on each transcript.

TABLE 110 Segment location on transcripts Segment Segment Transcript starting ending name position position HUMTEN_PEA_1_T4 6364 6410 (SEQ ID NO: 857) HUMTEN_PEA_1_T5 10571 10617 (SEQ ID NO: 858) HUMTEN_PEA_1_T6 7665 7711 (SEQ ID NO: 859) HUMTEN_PEA_1_T7 5815 5861 (SEQ ID NO: 860) HUMTEN_PEA_1_T11 5815 5861 (SEQ ID NO: 861) HUMTEN_PEA_1_T14 5269 5315 (SEQ ID NO: 862) HUMTEN_PEA_1_T16 4996 5042 (SEQ ID NO: 863) HUMTEN_PEA_1_T17 6088 6134 (SEQ ID NO: 864) HUMTEN_PEA_1_T18 4450 4496 (SEQ ID NO: 865) HUMTEN_PEA_1_T19 4177 4223 (SEQ ID NO: 866) HUMTEN_PEA_1_T20 6088 6134 (SEQ ID NO. 867) HUMTEN_PEA_1_T23 6088 6134 (SEQ ID NO: 868)

Segment cluster HUMTEN_PEA_(—)1_node_(—)67 (SEQ ID NO: 923) according to the present invention is supported by 92 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HUMTEN_PEA_(—)1_T4 (SEQ ID NO: 857), HUMTEN_PEA_(—)1_T5 (SEQ ID NO: 858), HUMTEN_PEA_(—)1_T6 (SEQ ID NO: 859), HUMTEN_PEA_(—)1_T7 (SEQ ID NO: 860), HUMTEN_PEA_(—)1_T11 (SEQ ID NO: 861), HUMTEN_PEA_(—)1_T14 (SEQ ID NO: 862), HUMTEN_PEA_(—)1_T16 (SEQ ID NO: 863), HUMTEN_PEA_(—)1_T17 (SEQ ID NO: 864), HUMTEN_PEA_(—)1_T18 (SEQ ID NO: 865), HUMTEN_PEA_(—)1_T19 (SEQ ID NO: 866), HUMTEN_PEA_(—)1_T20 (SEQ ID NO. 867) and HUMTEN_PEA_(—)1_T23 (SEQ ID NO: 868). Table 111 below describes the starting and ending position of this segment on each transcript.

TABLE 111 Segment location on transcripts Segment Segment Transcript starting ending name position position HUMTEN_PEA_1_T4 6544 6587 (SEQ ID NO: 857) HUMTEN_PEA_1_T5 10751 10794 (SEQ ID NO: 858) HUMTEN_PEA_1_T6 7845 7888 (SEQ ID NO: 859) HUMTEN_PEA_1_T7 5995 6038 (SEQ ID NO: 860) HUMTEN_PEA_1_T11 5995 6038 (SEQ ID NO: 861) HUMTEN_PEA_1_T14 5449 5492 (SEQ ID NO: 862) HUMTEN_PEA_1_T16 5176 5219 (SEQ ID NO: 863) HUMTEN_PEA_1_T17 6268 6311 (SEQ ID NO: 864) HUMTEN_PEA_1_T18 4630 4673 (SEQ ID NO: 865) HUMTEN_PEA_1_T19 4357 4400 (SEQ ID NO: 866) HUMTEN_PEA_1_T20 6268 6311 (SEQ ID NO. 867) HUMTEN_PEA_1_T23 6268 6311 (SEQ ID NO: 868)

Segment cluster HUMTEN_PEA_(—)1_node_(—)68 (SEQ ID NO: 924) according to the present invention is supported by 117 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HUMTEN_PEA_(—)1_T4 (SEQ ID NO: 857), HUMTEN_PEA_(—)1_T5 (SEQ ID NO: 858), HUMTEN_PEA_(—)1_T6 (SEQ ID NO: 859), HUMTEN_PEA_(—)1_T7 (SEQ ID NO: 860), HUMTEN_PEA_(—)1_T1 (SEQ ID NO: 861), HUMTEN_PEA_(—)1_T14 (SEQ ID NO: 862), HUMTEN_PEA_(—)1_T16 (SEQ ID NO: 863), HUMTEN_PEA_(—)1_T17 (SEQ ID NO: 864), HUMTEN_PEA_(—)1_T18 (SEQ ID NO: 865), HUMTEN_PEA_(—)1_T19 (SEQ ID NO: 866), HUMTEN_PEA_(—)1_T20 (SEQ ID NO. 867) and HUMTEN_PEA_(—)1_T23 (SEQ ID NO: 868). Table 112 below describes the starting and ending position of this segment on each transcript.

TABLE 112 Segment location on transcripts Segment Segment Transcript starting ending name position position HUMTEN_PEA_1_T4 6588 6668 (SEQ ID NO: 857) HUMTEN_PEA_1_T5 10795 10875 (SEQ ID NO: 858) HUMTEN_PEA_1_T6 7889 7969 (SEQ ID NO: 859) HUMTEN_PEA_1_T7 6039 6119 (SEQ ID NO: 860) HUMTEN_PEA_1_T11 6039 6119 (SEQ ID NO: 861) HUMTEN_PEA_1_T14 5493 5573 (SEQ ID NO: 862) HUMTEN_PEA_1_T16 5220 5300 (SEQ ID NO: 863) HUMTEN_PEA_1_T17 6312 6392 (SEQ ID NO: 864) HUMTEN_PEA_1_T18 4674 4754 (SEQ ID NO: 865) HUMTEN_PEA_1_T19 4401 4481 (SEQ ID NO: 866) HUMTEN_PEA_1_T20 6312 6392 (SEQ ID NO. 867) HUMTEN_PEA_1_T23 6312 6392 (SEQ ID NO: 868)

Segment cluster HUMTEN_PEA_(—)1_node_(—)69 (SEQ ID NO: 925) according to the present invention can be found in the following transcript(s): HUMTEN_PEA_(—)1_T4 (SEQ ID NO: 857), HUMTEN_PEA_(—)1_T5 (SEQ ID NO: 858), HUMTEN_PEA_(—)1_T6 (SEQ ID NO: 859), HUMTEN_PEA_(—)1_T7 (SEQ ID NO: 860), HUMTEN_PEA_(—)1_T11 (SEQ ID NO: 861), HUMTEN_PEA_(—)1_T14 (SEQ ID NO: 862), HUMTEN_PEA_(—)1_T16 (SEQ ID NO: 863), HUMTEN_PEA_(—)1_T17 (SEQ ID NO: 864), HUMTEN_PEA_(—)1_T18 (SEQ ID NO: 865), HUMTEN_PEA_(—)1_T19 (SEQ ID NO: 866), HUMTEN_PEA_(—)1_T20 (SEQ ID NO. 867) and HUMTEN_PEA_(—)1_T23 (SEQ ID NO: 868). Table 113 below describes the starting and ending position of this segment on each transcript.

TABLE 113 Segment location on transcripts Segment Segment Transcript starting ending name position position HUMTEN_PEA_1_T4 6669 6673 (SEQ ID NO: 857) HUMTEN_PEA_1_T5 10876 10880 (SEQ ID NO: 858) HUMTEN_PEA_1_T6 7970 7974 (SEQ ID NO: 859) HUMTEN_PEA_1_T7 6120 6124 (SEQ ID NO: 860) HUMTEN_PEA_1_T11 6120 6124 (SEQ ID NO: 861) HUMTEN_PEA_1_T14 5574 5578 (SEQ ID NO: 862) HUMTEN_PEA_1_T16 5301 5305 (SEQ ID NO: 863) HUMTEN_PEA_1_T17 6393 6397 (SEQ ID NO: 864) HUMTEN_PEA_1_T18 4755 4759 (SEQ ID NO: 865) HUMTEN_PEA_1_T19 4482 4486 (SEQ ID NO: 866) HUMTEN_PEA_1_T20 6393 6397 (SEQ ID NO. 867) HUMTEN_PEA_1_T23 6393 6397 (SEQ ID NO: 868)

Segment cluster HUMTEN_PEA_(—)1_node_(—)70 (SEQ ID NO: 926) according to the present invention can be found in the following transcript(s): HUMTEN_PEA_(—)1_T4 (SEQ ID NO: 857), HUMTEN_PEA_(—)1_T5 (SEQ ID NO: 858), HUMTEN_PEA_(—)1_T6 (SEQ ID NO: 859), HUMTEN_PEA_(—)1_T7 (SEQ ID NO: 860), HUMTEN_PEA_(—)1_T1 (SEQ ID NO: 861), HUMTEN_PEA_(—)1_T14 (SEQ ID NO: 862), HUMTEN_PEA_(—)1_T16 (SEQ ID NO: 863), HUMTEN_PEA_(—)1_T17 (SEQ ID NO: 864), HUMTEN_PEA_(—)1_T18 (SEQ ID NO: 865), HUMTEN_PEA_(—)1_T19 (SEQ ID NO: 866), HUMTEN_PEA_(—)1_T20 (SEQ ID NO. 867) and HUMTEN_PEA_(—)1_T23 (SEQ ID NO: 868). Table 114 below describes the starting and ending position of this segment on each transcript.

TABLE 114 Segment location on transcripts Segment Segment Transcript starting ending name position position HUMTEN_PEA_1_T4 6674 6695 (SEQ ID NO: 857) HUMTEN_PEA_1_T5 10881 10902 (SEQ ID NO: 858) HUMTEN_PEA_1_T6 7975 7996 (SEQ ID NO: 859) HUMTEN_PEA_1_T7 6125 6146 (SEQ ID NO: 860) HUMTEN_PEA_1_T11 6125 6146 (SEQ ID NO: 861) HUMTEN_PEA_1_T14 5579 5600 (SEQ ID NO: 862) HUMTEN_PEA_1_T16 5306 5327 (SEQ ID NO: 863) HUMTEN_PEA_1_T17 6398 6419 (SEQ ID NO: 864) HUMTEN_PEA_1_T18 4760 4781 (SEQ ID NO: 865) HUMTEN_PEA_1_T19 4487 4508 (SEQ ID NO: 866) HUMTEN_PEA_1_T20 6398 6419 (SEQ ID NO. 867) HUMTEN_PEA_1_T23 6398 6419 (SEQ ID NO: 868)

Segment cluster HUMTEN_PEA_(—)1_node_(—)72 (SEQ ID NO: 927) according to the present invention is supported by 121 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HUMTEN_PEA_(—)1_T4 (SEQ ID NO: 857), HUMTEN_PEA_(—)1_T5 (SEQ ID NO: 858), HUMTEN_PEA_(—)1_T6 (SEQ ID NO: 859), HUMTEN_PEA_(—)1_T7 (SEQ ID NO: 860), HUMTEN_PEA_(—)1_T11 (SEQ ID NO: 861), HUMTEN_PEA_(—)1_T14 (SEQ ID NO: 862), HUMTEN_PEA_(—)1_T16 (SEQ ID NO: 863), HUMTEN_PEA_(—)1_T7 (SEQ ID NO: 864), HUMTEN_PEA_(—)1_T18 (SEQ ID NO: 865), HUMTEN_PEA_(—)1_T19 (SEQ ID NO: 866) and HUMTEN_PEA_(—)1_T23 (SEQ ID NO: 868). Table 115 below describes the starting and ending position of this segment on each transcript.

TABLE 115 Segment location on transcripts Segment Segment Transcript starting ending name position position HUMTEN_PEA_1_T4 6696 6792 (SEQ ID NO: 857) HUMTEN_PEA_1_T5 10903 10999 (SEQ ID NO: 858) HUMTEN_PEA_1_T6 7997 8093 (SEQ ID NO: 859) HUMTEN_PEA_1_T7 6147 6243 (SEQ ID NO: 860) HUMTEN_PEA_1_T11 6147 6243 (SEQ ID NO: 861) HUMTEN_PEA_1_T14 5601 5697 (SEQ ID NO: 862) HUMTEN_PEA_1_T16 5328 5424 (SEQ ID NO: 863) HUMTEN_PEA_1_T17 7217 7313 (SEQ ID NO: 864) HUMTEN_PEA_1_T18 4782 4878 (SEQ ID NO: 865) HUMTEN_PEA_1_T19 4509 4605 (SEQ ID NO: 866) HUMTEN_PEA_1_T23 6420 6516 (SEQ ID NO: 868)

Segment cluster HUMTEN_PEA_(—)1_node_(—)84 (SEQ ID NO: 928) according to the present invention is supported by 153 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HUMTEN_PEA_(—)1_T4 (SEQ ID NO: 857), HUMTEN_PEA_(—)1_T5 (SEQ ID NO: 858), HUMTEN_PEA_(—)1_T6 (SEQ ID NO: 859), HUMTEN_PEA_(—)1_T7 (SEQ ID NO: 860), HUMTEN_PEA_(—)1_T11 (SEQ ID NO: 861), HUMTEN_PEA_(—)1_T14 (SEQ ID NO: 862), HUMTEN_PEA_(—)1_T16 (SEQ ID NO: 863), HUMTEN_PEA_(—)1_T17 (SEQ ID NO: 864), HUMTEN_PEA_(—)1_T18 (SEQ ID NO: 865), HUMTEN_PEA_(—)1_T19 (SEQ ID NO: 866) and HUMTEN_PEA_(—)1_T20 (SEQ ID NO. 867). Table 116 below describes the starting and ending position of this segment on each transcript.

TABLE 116 Segment location on transcripts Segment Segment Transcript starting ending name position position HUMTEN_PEA_1_T4 7241 7292 (SEQ ID NO: 857) HUMTEN_PEA_1_T5 11448 11499 (SEQ ID NO: 858) HUMTEN_PEA_1_T6 8542 8593 (SEQ ID NO: 859) HUMTEN_PEA_1_T7 6692 6743 (SEQ ID NO: 860) HUMTEN_PEA_1_T11 6692 6743 (SEQ ID NO: 861) HUMTEN_PEA_1_T14 6146 6197 (SEQ ID NO: 862) HUMTEN_PEA_1_T16 5873 5924 (SEQ ID NO: 863) HUMTEN_PEA_1_T17 7762 7813 (SEQ ID NO: 864) HUMTEN_PEA_1_T18 5327 5378 (SEQ ID NO: 865) HUMTEN_PEA_1_T19 5054 5105 (SEQ ID NO: 866) HUMTEN_PEA_1_T20 6706 6757 (SEQ ID NO. 867)

Segment cluster HUMTEN_PEA_(—)1_node_(—)85 (SEQ ID NO: 929) according to the present invention is supported by 168 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HUMTEN_PEA_(—)1_T4 (SEQ ID NO: 857), HUMTEN_PEA_(—)1_T5 (SEQ ID NO: 858), HUMTEN_PEA_(—)1_T6 (SEQ ID NO: 859), HUMTEN_PEA_(—)1_T7 (SEQ ID NO: 860), HUMTEN_PEA_(—)1_T11 (SEQ ID NO: 861), HUMTEN_PEA_(—)1_T14 (SEQ ID NO: 862), HUMTEN_PEA_(—)1_T16 (SEQ ID NO: 863), HUMTEN_PEA_(—)1_T17 (SEQ ID NO: 864), HUMTEN_PEA_(—)1_T18 (SEQ ID NO: 865), HUMTEN_PEA_(—)1_T19 (SEQ ID NO: 866) and HUMTEN_PEA_(—)1_T20 (SEQ ID NO. 867). Table 117 below describes the starting and ending position of this segment on each transcript.

TABLE 117 Segment location on transcripts Segment Segment Transcript starting ending name position position HUMTEN_PEA_1_T4 7293 7350 (SEQ ID NO: 857) HUMTEN_PEA_1_T5 11500 11557 (SEQ ID NO: 858) HUMTEN_PEA_1_T6 8594 8651 (SEQ ID NO: 859) HUMTEN_PEA_1_T7 6744 6801 (SEQ ID NO: 860) HUMTEN_PEA_1_T11 6744 6801 (SEQ ID NO: 861) HUMTEN_PEA_1_T14 6198 6255 (SEQ ID NO: 862) HUMTEN_PEA_1_T16 5925 5982 (SEQ ID NO: 863) HUMTEN_PEA_1_T17 7814 7871 (SEQ ID NO: 864) HUMTEN_PEA_1_T18 5379 5436 (SEQ ID NO: 865) HUMTEN_PEA_1_T19 5106 5163 (SEQ ID NO: 866) HUMTEN_PEA_1_T20 6758 6815 (SEQ ID NO. 867)

Segment cluster HUMTEN_PEA_(—)1_node_(—)86 (SEQ ID NO: 930) according to the present invention is supported by 179 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HUMTEN_PEA_(—)1_T4 (SEQ ID NO: 857), HUMTEN_PEA_(—)1_T5 (SEQ ID NO: 858), HUMTEN_PEA_(—)1_T6 (SEQ ID NO: 859), HUMTEN_PEA_(—)1_T7 (SEQ ID NO: 860), HUMTEN_PEA_(—)1_T11 (SEQ ID NO: 861), HUMTEN_PEA_(—)1_T14 (SEQ ID NO: 862), HUMTEN_PEA_(—)1_T16 (SEQ ID NO: 863), HUMTEN_PEA_(—)1_T17 (SEQ ID NO: 864), HUMTEN_PEA_(—)1_T18 (SEQ ID NO: 865), HUMTEN_PEA_(—)1_T19 (SEQ ID NO: 866) and HUMTEN_PEA_(—)1_T20 (SEQ ID NO. 867). Table 118 below describes the starting and ending position of this segment on each transcript.

TABLE 118 Segment location on transcripts Segment Segment Transcript starting ending name position position HUMTEN_PEA_1_T4 7351 7441 (SEQ ID NO: 857) HUMTEN_PEA_1_T5 11558 11648 (SEQ ID NO: 858) HUMTEN_PEA_1_T6 8652 8742 (SEQ ID NO: 859) HUMTEN_PEA_1_T7 6802 6892 (SEQ ID NO: 860) HUMTEN_PEA_1_T11 6802 6892 (SEQ ID NO: 861) HUMTEN_PEA_1_T14 6256 6346 (SEQ ID NO: 862) HUMTEN_PEA_1_T16 5983 6073 (SEQ ID NO: 863) HUMTEN_PEA_1_T17 7872 7962 (SEQ ID NO: 864) HUMTEN_PEA_1_T18 5437 5527 (SEQ ID NO: 865) HUMTEN_PEA_1_T19 5164 5254 (SEQ ID NO: 866) HUMTEN_PEA_1_T20 6816 6906 (SEQ ID NO. 867)

Segment cluster HUMTEN_PEA_(—)1_node_(—)87 (SEQ ID NO: 931) according to the present invention is supported by 167 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HUMTEN_PEA_(—)1_T4 (SEQ ID NO: 857), HUMTEN_PEA_(—)1_T5 (SEQ ID NO: 858), HUMTEN_PEA_(—)1_T6 (SEQ ID NO: 859), HUMTEN_PEA_(—)1_T7 (SEQ ID NO: 860), HUMTEN_PEA_(—)1_T11 (SEQ ID NO: 861), HUMTEN_PEA_(—)1_T14 (SEQ ID NO: 862), HUMTEN_PEA_(—)1_T16 (SEQ ID NO: 863), HUMTEN_PEA_(—)1_T17 (SEQ ID NO: 864), HUMTEN_PEA_(—)1_T18 (SEQ ID NO: 865), HUMTEN_PEA_(—)1_T19 (SEQ ID NO: 866) and HUMTEN_PEA_(—)1_T20 (SEQ ID NO. 867). Table 119 below describes the starting and ending position of this segment on each transcript.

TABLE 119 Segment location on transcripts Segment Segment Transcript starting ending name position position HUMTEN_PEA_1_T4 7442 7499 (SEQ ID NO: 857) HUMTEN_PEA_1_T5 11649 11706 (SEQ ID NO: 858) HUMTEN_PEA_1_T6 8743 8800 (SEQ ID NO: 859) HUMTEN_PEA_1_T7 6893 6950 (SEQ ID NO: 860) HUMTEN_PEA_1_T11 6893 6950 (SEQ ID NO: 861) HUMTEN_PEA_1_T14 6347 6404 (SEQ ID NO: 862) HUMTEN_PEA_1_T16 6074 6131 (SEQ ID NO: 863) HUMTEN_PEA_1_T17 7963 8020 (SEQ ID NO: 864) HUMTEN_PEA_1_T18 5528 5585 (SEQ ID NO: 865) HUMTEN_PEA_1_T19 5255 5312 (SEQ ID NO: 866) HUMTEN_PEA_1_T20 6907 6964 (SEQ ID NO. 867)

Segment cluster HUMTEN_PEA_(—)1_node_(—)88 (SEQ ID NO: 932) according to the present invention is supported by 164 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HUMTEN_PEA_(—)1_T4 (SEQ ID NO: 857), HUMTEN_PEA_(—)1_T5 (SEQ ID NO: 858), HUMTEN_PEA_(—)1_T6 (SEQ ID NO: 859), HUMTEN_PEA_(—)1_T7 (SEQ ID NO: 860), HUMTEN_PEA_(—)1_T11 (SEQ ID NO: 861), HUMTEN_PEA_(—)1_T14 (SEQ ID NO: 862), HUMTEN_PEA_(—)1_T16 (SEQ ID NO: 863), HUMTEN_PEA_(—)1_T17 (SEQ ID NO: 864), HUMTEN_PEA_(—)1_T18 (SEQ ID NO: 865), HUMTEN_PEA_(—)1_T19 (SEQ ID NO: 866) and HUMTEN_PEA_(—)1_T20 (SEQ ID NO. 867). Table 120 below describes the starting and ending position of this segment on each transcript.

TABLE 120 Segment location on transcripts Segment Segment Transcript starting ending name position position HUMTEN_PEA_1_T4 7500 7558 (SEQ ID NO: 857) HUMTEN_PEA_1_T5 11707 11765 (SEQ ID NO: 858) HUMTEN_PEA_1_T6 8801 8859 (SEQ ID NO: 859) HUMTEN_PEA_1_T7 6951 7009 (SEQ ID NO: 860) HUMTEN_PEA_1_T11 6951 7009 (SEQ ID NO: 861) HUMTEN_PEA_1_T14 6405 6463 (SEQ ID NO: 862) HUMTEN_PEA_1_T16 6132 6190 (SEQ ID NO: 863) HUMTEN_PEA_1_T17 8021 8079 (SEQ ID NO: 864) HUMTEN_PEA_1_T18 5586 5644 (SEQ ID NO: 865) HUMTEN_PEA_1_T19 5313 5371 (SEQ ID NO: 866) HUMTEN_PEA_1_T20 6965 7023 (SEQ ID NO. 867) Variant protein alignment to the previously known protein: Sequence name: TENA_HUMAN_V1 Sequence documentation:

Alignment of: HUMTEN_PEA_(—)1_P5 (SEQ ID NO: 934) x TENA_HUMAN_V1 ..

Alignment segment 1/1:

Quality: 21611.00 Escore: 0 Matching length: 2201 Total length: 2293 Matching Percent Similarity: 100.00 Matching Percent Identity: 100.00 Total Percent Similarity: 95.99 Total Percent Identity: 95.99 Gaps: 1

Alignment:

Sequence name: TENA_HUMAN_V1 Sequence documentation:

Alignment of: HUMTEN_PEA_(—)1_P6 (SEQ ID NO: 935) x TENA_HUMAN_V1 ..

Alignment segment 1/1:

Quality: 15349.00 Escore: 0 Matching length: 1603 Total length: 1603 Matching Percent Similarity: 97.75 Matching Percent Identity: 96.88 Total Percent Similarity: 97.75 Total Percent Identity: 96.88 Gaps: 0

Alignment:

Sequence name: TENA_HUMAN_V1 Sequence documentation:

Alignment of: HUMTEN_PEA_(—)1_P7 (SEQ ID NO: 936) x TENA_HUMAN_V1 ..

Alignment segment 1/1:

Quality: 16042.00 Escore: 0 Matching length: 1617 Total length: 1617 Matching Percent Similarity: 100.00 Matching Percent Identity: 100.00 Total Percent Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0

Alignment:

Sequence name: TENA_HUMAN_V1 Sequence documentation:

Alignment of: HUMTEN_PEA_(—)1_P8 (SEQ ID NO: 937) x TENA_HUMAN_V1 ..

Alignment segment 1/1:

Quality: 20743.00 Escore: 0 Matching length: 2110 Total length: 2201 Matching Percent Similarity: 100.00 Matching Percent Identity: 100.00 Total Percent Similarity: 95.87 Total Percent Identity: 95.87 Gaps: 1

Alignment:

Sequence name: TENA_HUMAN_V1 Sequence documentation:

Alignment of: HUMTEN_PEA_(—)1_P10 (SEQ ID NO: 938) x TENA_HUMAN_V1 ..

Alignment segment 1/1:

Quality: 20725.00 Escore: 0 Matching length: 2110 Total length: 2201 Matching Percent Similarity: 100.00 Matching Percent Identity: 100.00 Total Percent Similarity: 95.87 Total Percent Identity: 95.87 Gaps: 1

Alignment:

Sequence name: TENA_HUMAN_V1 Sequence documentation:

Alignment of: HUMTEN_PEA_(—)1_P11 (SEQ ID NO: 939) x TENA_HUMAN_V1 ..

Alignment segment 1/1:

Quality: 18990.00 Escore: 0 Matching length: 1928 Total length: 2201 Matching Percent Similarity: 100.00 Matching Percent Identity: 100.00 Total Percent Similarity: 87.60 Total Percent Identity: 87.60 Gaps: 1

Alignment:

Sequence name: TENA_HUMAN_V1 Sequence documentation:

Alignment of: HUMTEN_PEA_(—)1_P13 (SEQ ID NO: 940) x TENA_HUMAN_V1 ..

Alignment segment 1/1:

Quality: 18153.00 Escore: 0 Matching length: 1837 Total length: 2201 Matching Percent Similarity: 100.00 Matching Percent Identity: 100.00 Total Percent Similarity: 83.46 Total Percent Identity: 83.46 Gaps: 1

Alignment:

Sequence name: TENA_HUMAN_V1 Sequence documentation:

Alignment of: HUMTEN_PEA_(—)1_P14 (SEQ ID NO: 941) x TENA_HUMAN_V1 ..

Alignment segment 1/1:

Quality: 19930.00 Escore: 0 Matching length: 2025 Total length: 2025 Matching Percent Similarity: 100.00 Matching Percent Identity: 100.00 Total Percent Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0

Alignment:

Sequence name: TENA_HUMAN_V1 Sequence documentation:

Alignment of: HUMTEN_PEA_(—)1_P15 (SEQ ID NO: 942) x TENA_HUMAN_V1 ..

Alignment segment 1/1:

Quality: 16391.00 Escore: 0 Matching length: 1655 Total length: 2201 Matching Percent Similarity: 100.00 Matching Percent Identity: 100.00 Total Percent Similarity: 75.19 Total Percent Identity: 75.19 Gaps: 1

Alignment:

Sequence name: TENA_HUMAN_V1 Sequence documentation:

Alignment of: HUMTEN_PEA_(—)1_P16 (SEQ ID NO: 943) x TENA_HUMAN_V1 ..

Alignment segment 1/1:

Quality: 15530.00 Escore: 0 Matching length: 1564 Total length: 2201 Matching Percent Similarity: 100.00 Matching Percent Identity: 100.00 Total Percent Similarity: 71.06 Total Percent Identity: 71.06 Gaps: 1

Alignment:

Sequence name: TENA_HUMAN_V1 Sequence documentation:

Alignment of: HUMTEN_PEA_(—)1_P17 (SEQ ID NO: 944) x TENA_HUMAN_V1 ..

Alignment segment 1/1:

Quality: 19930.00 Escore: 0 Matching length: 2025 Total length: 2025 Matching Percent Similarity: 100.00 Matching Percent Identity: 100.00 Total Percent Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0

Alignment:

Sequence name: TENA_HUMAN_V1 Sequence documentation:

Alignment of: HUMTEN_PEA_(—)1_P20 (SEQ ID NO: 945) x TENA_HUMAN_V1 ..

Alignment segment 1/1:

Quality: 20262.00 Escore: 0 Matching length: 2057 Total length: 2057 Matching Percent Similarity: 100.00 Matching Percent Identity: 100.00 Total Percent Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0

Alignment:

Sequence name: TENA_HUMAN_V1 Sequence documentation:

Alignment of: HUMTEN_PEA_(—)1_P26 (SEQ ID NO: 946) x TENA_HUMAN_V1 ..

Alignment segment 1/1:

Quality: 16903.00 Escore: 0 Matching length: 1708 Total length: 1708 Matching Percent Similarity: 100.00 Matching Percent Identity: 100.00 Total Percent Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0

Alignment:

Sequence name: TENA_HUMAN_V1 Sequence documentation:

Alignment of: HUMTEN_PEA_(—)1_P27 (SEQ ID NO: 947) x TENA_HUMAN_V1 ..

Alignment segment 1/1:

Quality: 13445.00 Escore: 0 Matching length: 1344 Total length: 1344 Matching Percent Similarity: 100.00 Matching Percent Identity: 100.00 Total Percent Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0

Alignment:

Sequence name: TENA_HUMAN_V1 Sequence documentation:

Alignment of: HUMTEN_PEA_(—)1_P28 (SEQ ID NO: 948) x TENA_HUMAN_V1 ..

Alignment segment 1/1:

Quality: 12559.00 Escore: 0 Matching length: 1253 Total length: 1253 Matching Percent Similarity: 100.00 Matching Percent Identity: 100.00 Total Percent Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0

Alignment:

Sequence name: TENA_HUMAN_V1 Sequence documentation:

Alignment of: HUMTEN_PEA_(—)1_P29 (SEQ ID NO: 949) x TENA_HUMAN_V1 ..

Alignment segment 1/1:

Quality: 10822.00 Escore: 0 Matching length: 1071 Total length: 1071 Matching Percent Similarity: 100.00 Matching Percent Identity: 100.00 Total Percent Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0

Alignment:

Sequence name: TENA_HUMAN_V1 Sequence documentation:

Alignment of: HUMTEN_PEA_(—)1_P30 (SEQ ID NO: 950) x TENA_HUMAN_V1 ..

Alignment segment 1/1:

Quality: 9694.00 Escore: 0 Matching length: 954 Total length: 954 Matching Percent Similarity: 100.00 Matching Percent Identity: 100.00 Total Percent Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0

Alignment:

Sequence name: TENA_HUMAN_V1 Sequence documentation:

Alignment of: HUMTEN_PEA_(—)1_P31 (SEQ ID NO: 951) x TENA_HUMAN_V1 ..

Alignment segment 1/1:

Quality: 8236.00 Escore: 0 Matching length: 802 Total length: 802 Matching Percent Similarity: 100.00 Matching Percent Identity: 100.00 Total Percent Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0

Alignment:

Sequence name: TENA_HUMAN_V1 Sequence documentation:

Alignment of: HUMTEN_PEA_(—)1_P32 (SEQ ID NO: 952) x TENA_HUMAN_V1 ..

Alignment segment 1/1:

Quality: 7332.00 Escore: 0 Matching length: 710 Total length: 710 Matching Percent Similarity: 100.00 Matching Percent Identity: 100.00 Total Percent Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0

Alignment:

Description for Cluster HUMOSTRO

Cluster HUMOSTRO features 3 transcript(s) and 30 segment(s) of interest, the names for which are given in Tables 1 and 2, respectively, the sequences themselves are given at the end of the application. The selected protein variants are given in table 3.

TABLE 1 Transcripts of interest SEQ ID Transcript Name NO: HUMOSTRO_PEA_1_PEA_1_T14 277 HUMOSTRO_PEA_1_PEA_1_T16 278 HUMOSTRO_PEA_1_PEA_1_T30 279

TABLE 2 Segments of interest SEQ ID Segment Name NO: HUMOSTRO_PEA_1_PEA_1_node_0 280 HUMOSTRO_PEA_1_PEA_1_node_10 281 HUMOSTRO_PEA_1_PEA_1_node_16 282 HUMOSTRO_PEA_1_PEA_1_node_23 283 HUMOSTRO_PEA_1_PEA_1_node_31 284 HUMOSTRO_PEA_1_PEA_1_node_43 285 HUMOSTRO_PEA_1_PEA_1_node_3 286 HUMOSTRO_PEA_1_PEA_1_node_5 287 HUMOSTRO_PEA_1_PEA_1_node_7 288 HUMOSTRO_PEA_1_PEA_1_node_8 289 HUMOSTRO_PEA_1_PEA_1_node_15 290 HUMOSTRO_PEA_1_PEA_1_node_17 291 HUMOSTRO_PEA_1_PEA_1_node_20 292 HUMOSTRO_PEA_1_PEA_1_node_21 293 HUMOSTRO_PEA_1_PEA_1_node_22 294 HUMOSTRO_PEA_1_PEA_1_node_24 295 HUMOSTRO_PEA_1_PEA_1_node_26 296 HUMOSTRO_PEA_1_PEA_1_node_27 297 HUMOSTRO_PEA_1_PEA_1_node_28 298 HUMOSTRO_PEA_1_PEA_1_node_29 299 HUMOSTRO_PEA_1_PEA_1_node_30 300 HUMOSTRO_PEA_1_PEA_1_node_32 301 HUMOSTRO_PEA_1_PEA_1_node_34 302 HUMOSTRO_PEA_1_PEA_1_node_36 303 HUMOSTRO_PEA_1_PEA_1_node_37 304 HUMOSTRO_PEA_1_PEA_1_node_38 305 HUMOSTRO_PEA_1_PEA_1_node_39 306 HUMOSTRO_PEA_1_PEA_1_node_40 307 HUMOSTRO_PEA_1_PEA_1_node_41 308 HUMOSTRO_PEA_1_PEA_1_node_42 309

TABLE 3 Proteins of interest SEQ ID Corresponding Protein Name NO: Transcript(s) HUMOSTRO_PEA_1_PEA_1_ 311 HUMOSTRO_PEA_1_PEA_1_T14 P21 (SEQ ID NO:277) HUMOSTRO_PEA_1_PEA_1_ 312 HUMOSTRO_PEA_1_PEA_1_T16 P25 (SEQ ID NO:278) HUMOSTRO_PEA_1_PEA_1_ 313 HUMOSTRO_PEA_1_PEA_1_T30 P30. (SEQ ID NO:279)

These sequences are variants of the known protein Osteopontin precursor (SwissProt accession identifier OSTP_HUMAN; known also according to the synonyms Bone sialoprotein 1; Urinary stone protein; Secreted phosphoprotein 1; SPP-1; Nephropontin; Uropontin), SEQ ID NO: 310, referred to herein as the previously known protein.

Protein Osteopontin precursor is known or believed to have the following function(s): binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction. Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity (By similarity). The sequence for protein Osteopontin precursor is given at the end of the application, as “Osteopontin precursor amino acid sequence”. Known polymorphisms for this sequence are as shown in Table 4.

TABLE 4 Amino acid mutations for Known Protein SNP position(s) on amino acid sequence Comment 301 R -> H (in dbSNP: 4660). /FTId = VAR_014717. 188 D -> H 237 T -> A 275-278 SHEF -> GNSL

Protein Osteopontin precursor localization is believed to be Secreted.

The previously known protein also has the following indication(s) and/or potential therapeutic use(s): Regeneration, bone. It has been investigated for clinical/therapeutic use in humans, for example as a target for an antibody or small molecule, and/or as a direct therapeutic; available information related to these investigations is as follows. Potential pharmaceutically related or therapeutically related activity or activities of the previously known protein are as follows: Bone formation stimulant. A therapeutic role for a protein represented by the cluster has been predicted. The cluster was assigned this field because there was information in the drug database or the public databases (e.g., described herein above) that this protein, or part thereof, is used or can be used for a potential therapeutic indication: Musculoskeletal.

The following GO Annotation(s) apply to the previously known protein. The following annotation(s) were found: ossification; anti-apoptosis; inflammatory response; cell-matrix adhesion; cell-cell signaling, which are annotation(s) related to Biological Process; defense/immunity protein; cytokine; integrin ligand; protein binding; growth factor; apoptosis inhibitor, which are annotation(s) related to Molecular Function; and extracellular matrix, which are annotation(s) related to Cellular Component.

The GO assignment relies on information from one or more of the SwissProt/TremBl Protein knowledgebase, available from <http://www.expasy.ch/sprot/>; or Locuslink, available from <http://www.ncbi.nlm.nih.gov/projects/LocusLink/>.

Cluster HUMOSTRO can be used as a diagnostic marker according to overexpression of transcripts of this cluster in cancer. Expression of such transcripts in normal tissues is also given according to the previously described methods. The term “number” in the left hand column of the table and the numbers on the y-axis of FIG. 38 refer to weighted expression of ESTs in each category, as “parts per million” (ratio of the expression of ESTs for a particular cluster to the expression of all ESTs in that category, according to parts per million).

Overall, the following results were obtained as shown with regard to the histograms in FIG. 38 and Table 5. This cluster is overexpressed (at least at a minimum level) in the following pathological conditions: epithelial malignant tumors, a mixture of malignant tumors from different tissues, lung malignant tumors, breast malignant tumors, ovarian carcinoma and skin malignancies.

TABLE 5 Normal tissue distribution Name of Tissue Number adrenal 4 bladder 0 bone 897 brain 506 colon 69 epithelial 548 general 484 head and neck 50 kidney 5618 liver 4 lung 10 lymph nodes 75 breast 8 bone marrow 62 muscle 37 ovary 40 pancreas 845 prostate 48 skin 13 stomach 73 Thyroid 0 uterus 168

TABLE 6 P values and ratios for expression in cancerous tissue Name of Tissue P1 P2 SP1 R3 SP2 R4 adrenal 1.5e−01 2.1e−01 2.0e−02 4.6 4.4e−02 3.6 bladder 1.2e−01 9.2e−02 5.7e−02 4.1 2.1e−02 4.3 bone 4.9e−01 7.4e−01 4.1e−06 0.6 5.4e−01 0.4 brain 6.6e−01 7.0e−01 3.2e−01 0.6 1 0.4 colon 2.7e−01 4.0e−01 3.1e−01 1.5 5.2e−01 1.1 epithelial 2.0e−07 1.6e−03 9.8e−01 0.7 1 0.5 general 1.2e−06 1.2e−02 7.9e−01 0.8 1 0.6 head and neck 3.4e−01 5.0e−01 1 0.7 1 0.7 kidney 6.8e−01 7.4e−01 1 0.2 1 0.1 liver 3.3e−01 2.5e−01 1 1.8 2.3e−01 2.6 lung 4.3e−04 4.6e−03 2.1e−30 15.0 2.8e−27 23.5 lymph nodes 6.7e−01 8.7e−01 8.1e−01 0.7 9.9e−01 0.3 breast 2.3e−01 3.0e−01 1.9e−04 6.2 4.1e−03 4.3 bone marrow 7.5e−01 7.8e−01 1 0.3 2.0e−02 1.2 muscle 4.0e−02 7.5e−02 1.1e−01 4.6 5.1e−01 1.5 ovary 4.7e−02 8.4e−02 1.9e−05 5.4 8.3e−04 3.7 pancreas 5.0e−02 3.3e−01 1 0.3 1 0.2 prostate 8.5e−01 9.0e−01 8.9e−01 0.7 9.5e−01 0.6 skin 1.6e−01 1.6e−01 1.2e−10 12.6 5.2e−04 4.1 stomach 1.5e−01 6.3e−01 5.0e−01 1.2 9.4e−01 0.6 Thyroid 2.9e−01 2.9e−01 5.9e−02 2.0 5.9e−02 2.0 uterus 6.1e−02 5.7e−01 1.1e−01 1.3 7.0e−01 0.7

As noted above, cluster HUMOSTRO features 3 transcript(s), which were listed in Table 1 above. These transcript(s) encode for protein(s) which are variant(s) of protein Osteopontin precursor. A description of each variant protein according to the present invention is now provided.

Variant protein HUMOSTRO_PEA_(—)1_PEA_(—)1_P21 (SEQ ID NO: 311) according to the present invention has an amino acid sequence as given at the end of the application; it is encoded by transcript(s) HUMOSTRO_PEA_(—)1_PEA_(—)1_T14 (SEQ ID NO: 277). An alignment is given to the known protein (Osteopontin precursor) at the end of the application. One or more alignments to one or more previously published protein sequences are given at the end of the application. A brief description of the relationship of the variant protein according to the present invention to each such aligned protein is as follows:

Comparison report between HUMOSTRO_PEA_(—)1_PEA_(—)1_P21 (SEQ ID NO: 311) and OSTP_HUMAN:

1. An isolated chimeric polypeptide encoding for HUMOSTRO_PEA_(—)1_PEA_(—)1_P21 (SEQ ID NO: 311), comprising a first amino acid sequence being at least 90% homologous to MRIAVICFCLLGITCAIPVKQADSGSSEEKQLYNKYPDAVATWLNPDPSQKQNLLAPQ corresponding to amino acids 1-58 of OSTP_HUMAN, which also corresponds to amino acids 1-58 of HUMOSTRO_PEA_(—)1_PEA_(—)1_P21 (SEQ ID NO: 311), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence VFLNFS (SEQ ID NO: 1108) corresponding to amino acids 59-64 of HUMOSTRO_PEA_(—)1_PEA_(—)1_P21 (SEQ ID NO: 311), wherein said first amino acid sequence and second amino acid sequence are contiguous and in a sequential order.

2. An isolated polypeptide encoding for a tail of HUMOSTRO_PEA_(—)1_PEA_(—)1_P21 (SEQ ID NO: 311), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence VFLNFS (SEQ ID NO: 1108) in HUMOSTRO_PEA_(—)1_PEA_(—)1_P21 (SEQ ID NO: 311).

The location of the variant protein was determined according to results from a number of different software programs and analyses, including analyses from SignalP and other specialized programs. The variant protein is believed to be located as follows with regard to the cell: secreted. The protein localization is believed to be secreted because of manual inspection of known protein localization and/or gene structure.

Variant protein HUMOSTRO_PEA_(—)1_PEA_(—)1_P21 (SEQ ID NO: 311) also has the following non-silent SNPs (Single Nucleotide Polymorphisms) as listed in Table 7, (given according to their position(s) on the amino acid sequence, with the alternative amino acid(s) listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein HUMOSTRO_PEA_(—)1_PEA_(—)1_P21 (SEQ ID NO: 311) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 7 Amino acid mutations SNP position(s) on amino acid sequence Alternative amino acid(s) Previously known SNP? 7 C -> W No 31 Q -> R No 47 D -> V Yes 49 S -> P No

The glycosylation sites of variant protein HUMOSTRO_PEA_(—)1_PEA_(—)1_P21 (SEQ ID NO: 311), as compared to the known protein Osteopontin precursor, are described in Table 8 (given according to their position(s) on the amino acid sequence in the first column; the second column indicates whether the glycosylation site is present in the variant protein; and the last column indicates whether the position is different on the variant protein).

TABLE 8 Glycosylation site(s) Position(s) on known amino acid sequence Present in variant protein? 79 no 106 no

Variant protein HUMOSTRO_PEA_(—)1_PEA_(—)1_P21 (SEQ ID NO: 311) is encoded by the following transcript(s): HUMOSTRO_PEA_(—)1_PEA_(—)1_T14 (SEQ ID NO: 277), for which the sequence(s) is/are given at the end of the application. The coding portion of transcript HUMOSTRO_PEA_(—)1_PEA_(—)1_T14 (SEQ ID NO: 277) is shown in bold; this coding portion starts at position 199 and ends at position 390. The transcript also has the following SNPs as listed in Table 9 (given according to their position on the nucleotide sequence, with the alternative nucleic acid listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein HUMOSTRO_PEA_(—)1_PEA_(—)1_P21 (SEQ ID NO: 311) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 9 Nucleic acid SNPs SNP position on nucleotide sequence Alternative nucleic acid Previously known SNP? 136 A -> G Yes 154 T -> No 159 G -> T Yes 219 C -> G No 274 -> G No 290 A -> G No 338 A -> T Yes 343 T -> C No 413 G -> C Yes 707 C -> T Yes 708 C -> A Yes 715 A -> G Yes 730 A -> C No 730 A -> G No 746 T -> C Yes 767 C -> T No 779 G -> A Yes 866 -> G No 869 T -> No 889 -> A No 891 A -> C No 891 A -> G No 905 T -> C No 910 -> G No 910 -> T No 997 A -> G No 1026 G -> C No 1042 -> G No 1042 -> T No 1071 A -> No 1071 A -> C No 1098 A -> No 1105 C -> T No 1124 -> G No 1135 G -> A Yes 1136 T -> No 1136 T -> G No 1173 A -> C No 1173 A -> G No 1179 A -> G No 1214 C -> T Yes 1246 T -> No 1246 T -> A No 1359 A -> No 1359 A -> G No 1362 T -> No 1365 C -> T Yes 1366 G -> A Yes 1408 A -> C No 1418 A -> C No 1433 A -> C No 1456 A -> C No 1524 T -> A No 1524 T -> C No 1547 A -> G Yes 1553 T -> No 1574 -> G No 1654 A -> C Yes 1691 A -> G No 1703 A -> C Yes 1755 A -> C No 1764 T -> No

Variant protein HUMOSTRO_PEA_(—)1_PEA_(—)1_P25 (SEQ ID NO: 312) according to the present invention has an amino acid sequence as given at the end of the application; it is encoded by transcript(s) HUMOSTRO_PEA_(—)1_PEA_(—)1_T16 (SEQ ID NO: 278). An alignment is given to the known protein (Osteopontin precursor) at the end of the application. One or more alignments to one or more previously published protein sequences are given at the end of the application. A brief description of the relationship of the variant protein according to the present invention to each such aligned protein is as follows:

Comparison report between HUMOSTRO_PEA_(—)1_PEA_(—)1_P25 (SEQ ID NO: 312) and OSTP_HUMAN:

1. An isolated chimeric polypeptide encoding for HUMOSTRO_PEA_(—)1_PEA_(—)1_P25 (SEQ ID NO: 312), comprising a first amino acid sequence being at least 90% homologous to MRIAVICFCLLGITCAIPVKQADSGSSEEKQ corresponding to amino acids 1-31 of OSTP_HUMAN, which also corresponds to amino acids 1-31 of HUMOSTRO_PEA_(—)1_PEA_(—)1_P25 (SEQ ID NO: 312), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence H corresponding to amino acids 32-32 of HUMOSTRO_PEA_(—)1_PEA_(—)1_P25 (SEQ ID NO: 312), wherein said first amino acid sequence and second amino acid sequence are contiguous and in a sequential order.

The location of the variant protein was determined according to results from a number of different software programs and analyses, including analyses from SignalP and other specialized programs. The variant protein is believed to be located as follows with regard to the cell: secreted. The protein localization is believed to be secreted because both signal-peptide prediction programs predict that this protein has a signal peptide, and neither trans-membrane region prediction program predicts that this protein has a trans-membrane region.

Variant protein HUMOSTRO_PEA_(—)1_PEA_(—)1_P25 (SEQ ID NO: 312) also has the following non-silent SNPs (Single Nucleotide Polymorphisms) as listed in Table 10, (given according to their position(s) on the amino acid sequence, with the alternative amino acid(s) listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein HUMOSTRO_PEA_(—)1_PEA_(—)1_P25 (SEQ ID NO: 312) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 10 Amino acid mutations SNP position(s) on amino acid sequence Alternative amino acid(s) Previously known SNP? 7 C -> W No 31 Q -> R No

The glycosylation sites of variant protein HUMOSTRO_PEA_(—)1_PEA_(—)1_P25 (SEQ ID NO: 312), as compared to the known protein Osteopontin precursor, are described in Table 11 (given according to their position(s) on the amino acid sequence in the first column; the second column indicates whether the glycosylation site is present in the variant protein; and the last column indicates whether the position is different on the variant protein).

TABLE 11 Glycosylation site(s) Position(s) on known amino acid sequence Present in variant protein? 79 no 106 no

Variant protein HUMOSTRO_PEA_(—)1_PEA_(—)1_P25 (SEQ ID NO: 312) is encoded by the following transcript(s): HUMOSTRO_PEA_(—)1_PEA_(—)1_T16 (SEQ ID NO: 278), for which the sequence(s) is/are given at the end of the application. The coding portion of transcript HUMOSTRO_PEA_(—)1_PEA_(—)1_T16 (SEQ ID NO: 278) is shown in bold; this coding portion starts at position 199 and ends at position 294. The transcript also has the following SNPs as listed in Table 12 (given according to their position on the nucleotide sequence, with the alternative nucleic acid listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein HUMOSTRO_PEA_(—)1_PEA_(—)1_P25 (SEQ ID NO: 312) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 12 Nucleic acid SNPs SNP position on nucleotide sequence Alternative nucleic acid Previously known SNP? 136 A -> G Yes 154 T -> No 159 G -> T Yes 219 C -> G No 274 -> G No 290 A -> G No 419 C -> T Yes 454 G -> C Yes 527 A -> T Yes 532 T -> C No 630 C -> T Yes 631 C -> A Yes 638 A -> G Yes 653 A -> C No 653 A -> G No 669 T -> C Yes 690 C -> T No 702 G -> A Yes 789 -> G No 792 T -> No 812 -> A No 814 A -> C No 814 A -> G No 828 T -> C No 833 -> G No 833 -> T No 920 A -> G No 949 G -> C No 965 -> G No 965 -> T No 994 A -> No 994 A -> C No 1021 A -> No 1028 C -> T No 1047 -> G No 1058 G -> A Yes 1059 T -> No 1059 T -> G No 1096 A -> C No 1096 A -> G No 1102 A -> G No 1137 C -> T Yes 1169 T -> No 1169 T -> A No 1282 A -> No 1282 A -> G No 1285 T -> No 1288 C -> T Yes 1289 G -> A Yes 1331 A -> C No 1341 A -> C No 1356 A -> C No 1379 A -> C No 1447 T -> A No 1447 T -> C No 1470 A -> G Yes 1476 T -> No 1497 -> G No 1577 A -> C Yes 1614 A -> G No 1626 A -> C Yes 1678 A -> C No 1687 T -> No

Variant protein HUMOSTRO_PEA_(—)1_PEA_(—)1_P30 (SEQ ID NO: 313) according to the present invention has an amino acid sequence as given at the end of the application; it is encoded by transcript(s) HUMOSTRO_PEA_(—)1_PEA_(—)1_T30 (SEQ ID NO: 279). An alignment is given to the known protein (Osteopontin precursor) at the end of the application. One or more alignments to one or more previously published protein sequences are given at the end of the application. A brief description of the relationship of the variant protein according to the present invention to each such aligned protein is as follows:

Comparison report between HUMOSTRO_PEA_(—)1_PEA_(—)1_P30 (SEQ ID NO: 313) and OSTP_HUMAN:

1. An isolated chimeric polypeptide encoding for HUMOSTRO_PEA_(—)1_PEA_(—)1_P30 (SEQ ID NO: 313), comprising a first amino acid sequence being at least 90% homologous to MRIAVICFCLLGITCAIPVKQADSGSSEEKQ corresponding to amino acids 1-31 of OSTP_HUMAN, which also corresponds to amino acids 1-31 of HUMOSTRO_PEA_(—)1_PEA_(—)1_P30 (SEQ ID NO: 313), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence VSIFYVFI (SEQ ID NO: 1109) corresponding to amino acids 32-39 of HUMOSTRO_PEA_(—)1_PEA_(—)1_P30 (SEQ ID NO: 313), wherein said first amino acid sequence and second amino acid sequence are contiguous and in a sequential order.

2. An isolated polypeptide encoding for a tail of HUMOSTRO_PEA_(—)1_PEA_(—)1_P30 (SEQ ID NO: 313), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence VSIFYVFI (SEQ ID NO: 1109) in HUMOSTRO_PEA_(—)1_PEA_(—)1_P30 (SEQ ID NO: 313).

The location of the variant protein was determined according to results from a number of different software programs and analyses, including analyses from SignalP and other specialized programs. The variant protein is believed to be located as follows with regard to the cell: secreted. The protein localization is believed to be secreted because both signal-peptide prediction programs predict that this protein has a signal peptide, and neither trans-membrane region prediction program predicts that this protein has a trans-membrane region.

Variant protein HUMOSTRO_PEA_(—)1_PEA_(—)1_P30 (SEQ ID NO: 313) also has the following non-silent SNPs (Single Nucleotide Polymorphisms) as listed in Table 13, (given according to their position(s) on the amino acid sequence, with the alternative amino acid(s) listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein HUMOSTRO_PEA_(—)1_PEA_(—)1_P30 (SEQ ID NO: 313) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 13 Amino acid mutations SNP position(s) on amino acid sequence Alternative amino acid(s) Previously known SNP? 7 C -> W No 31 Q -> R No

The glycosylation sites of variant protein HUMOSTRO_PEA_(—)1_PEA_(—)1_P30 (SEQ ID NO: 313), as compared to the known protein Osteopontin precursor, are described in Table 14 (given according to their position(s) on the amino acid sequence in the first column; the second column indicates whether the glycosylation site is present in the variant protein; and the last column indicates whether the position is different on the variant protein).

TABLE 14 Glycosylation site(s) Position(s) on known amino acid sequence Present in variant protein? 79 no 106 no

Variant protein HUMOSTRO_PEA_(—)1_PEA_(—)1_P30 (SEQ ID NO: 313) is encoded by the following transcript(s): HUMOSTRO_PEA_(—)1_PEA_(—)1_T30 (SEQ ID NO: 279), for which the sequence(s) is/are given at the end of the application. The coding portion of transcript HUMOSTRO_PEA_(—)1_PEA_(—)1_T30 (SEQ ID NO: 279) is shown in bold; this coding portion starts at position 199 and ends at position 315. The transcript also has the following SNPs as listed in Table 15 (given according to their position on the nucleotide sequence, with the alternative nucleic acid listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein HUMOSTRO_PEA_(—)1_PEA_(—)1_P30 (SEQ ID NO: 313) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 15 Nucleic acid SNPs SNP position on nucleotide sequence Alternative nucleic acid Previously known SNP? 136 A -> G Yes 154 T -> No 159 G -> T Yes 219 C -> G No 274 -> G No 290 A -> G No

As noted above, cluster HUMOSTRO features 30 segment(s), which were listed in Table 2 above and for which the sequence(s) are given at the end of the application. These segment(s) are portions of nucleic acid sequence(s) which are described herein separately because they are of particular interest. A description of each segment according to the present invention is now provided.

Segment cluster HUMOSTRO_PEA_(—)1_PEA_(—)1_node_(—)0 (SEQ ID NO: 280) according to the present invention is supported by 333 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HUMOSTRO_PEA_(—)1_PEA_(—)1_T14 (SEQ ID NO: 277), HUMOSTRO_PEA_(—)1_PEA_(—)1_T16 (SEQ ID NO: 278) and HUMOSTRO_PEA_(—)1_PEA_(—)1_T30 (SEQ ID NO: 279). Table 16 below describes the starting and ending position of this segment on each transcript.

TABLE 16 Segment location on transcripts Segment Segment starting ending Transcript name position position HUMOSTRO_PEA_1_PEA_1_T14 1 184 (SEQ ID NO: 277) HUMOSTRO_PEA_1_PEA_1_T16 1 184 (SEQ ID NO: 278) HUMOSTRO_PEA_1_PEA_1_T30 1 184 (SEQ ID NO: 279)

Segment cluster HUMOSTRO_PEA_(—)1_PEA_(—)1_node_(—)10 (SEQ ID NO: 281) according to the present invention is supported by 4 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HUMOSTRO_PEA_(—)1_PEA_(—)1_T16 (SEQ ID NO: 278). Table 17 below describes the starting and ending position of this segment on each transcript.

TABLE 17 Segment location on transcripts Segment Segment starting ending Transcript name position position HUMOSTRO_PEA_1_PEA_1_T16 292 480 (SEQ ID NO: 278)

Segment cluster HUMOSTRO_PEA_(—)1_PEA_(—)1_node_(—)16 (SEQ ID NO: 282) according to the present invention is supported by 6 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HUMOSTRO_PEA_(—)1_PEA_(—)1_T14 (SEQ ID NO: 277). Table 18 below describes the starting and ending position of this segment on each transcript.

TABLE 18 Segment location on transcripts Segment Segment starting ending Transcript name position position HUMOSTRO_PEA_1_PEA_1_T14 373 638 (SEQ ID NO: 277)

Segment cluster HUMOSTRO_PEA_(—)1_PEA_(—)1_node_(—)23 (SEQ ID NO: 283) according to the present invention is supported by 334 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HUMOSTRO_PEA_(—)1_PEA_(—)1_T14 (SEQ ID NO: 277) and HUMOSTRO_PEA_(—)1_PEA_(—)1_T16 (SEQ ID NO: 278). Table 19 below describes the starting and ending position of this segment on each transcript.

TABLE 19 Segment location on transcripts Segment Segment starting ending Transcript name position position HUMOSTRO_PEA_1_PEA_1_T14 804 967 (SEQ ID NO: 277) HUMOSTRO_PEA_1_PEA_1_T16 727 890 (SEQ ID NO: 278)

Segment cluster HUMOSTRO_PEA_(—)1_PEA_(—)1_node_(—)31 (SEQ ID NO: 284) according to the present invention is supported by 350 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HUMOSTRO_PEA_(—)1_PEA_(—)1_T14 (SEQ ID NO: 277) and HUMOSTRO_PEA_(—)1_PEA_(—)1_T16 (SEQ ID NO: 278). Table 20 below describes the starting and ending position of this segment on each transcript.

TABLE 20 Segment location on transcripts Segment Segment starting ending Transcript name position position HUMOSTRO_PEA_1_PEA_1_T14 1164 1393 (SEQ ID NO: 277) HUMOSTRO_PEA_1_PEA_1_T16 1087 1316 (SEQ ID NO: 278)

Segment cluster HUMOSTRO_PEA_(—)1_PEA_(—)1_node_(—)43 (SEQ ID NO: 285) according to the present invention is supported by 192 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HUMOSTRO_PEA_(—)1_PEA_(—)1_T14 (SEQ ID NO: 277) and HUMOSTRO_PEA_(—)1_PEA_(—)1_T16 (SEQ ID NO: 278). Table 21 below describes the starting and ending position of this segment on each transcript.

TABLE 21 Segment location on transcripts Segment Segment starting ending Transcript name position position HUMOSTRO_PEA_1_PEA_1_T14 1810 1846 (SEQ ID NO: 277) HUMOSTRO_PEA_1_PEA_1_T16 1733 1769 (SEQ ID NO: 278)

According to an optional embodiment of the present invention, short segments related to the above cluster are also provided. These segments are up to about 120 bp in length, and so are included in a separate description.

Segment cluster HUMOSTRO_PEA_(—)1_PEA_(—)1_node_(—)3 according to the present invention is supported by 353 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HUMOSTRO_PEA_(—)1_PEA_(—)1_T14 (SEQ ID NO: 277), HUMOSTRO_PEA_(—)1_PEA_(—)1_T16 (SEQ ID NO: 278) and HUMOSTRO_PEA_(—)1_PEA_(—)1_T30 (SEQ ID NO: 279). Table 22 below describes the starting and ending position of this segment on each transcript.

TABLE 22 Segment location on transcripts Segment Segment starting ending Transcript name position position HUMOSTRO_PEA_1_PEA_1_T14 185 210 (SEQ ID NO: 277) HUMOSTRO_PEA_1_PEA_1_T16 185 210 (SEQ ID NO: 278) HUMOSTRO_PEA_1_PEA_1_T30 185 210 (SEQ ID NO: 279)

Segment cluster HUMOSTRO_PEA_(—)1_PEA_(—)1_node_(—)5 (SEQ ID NO: 287) according to the present invention is supported by 353 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HUMOSTRO_PEA_(—)1_PEA_(—)1_T14 (SEQ ID NO: 277), HUMOSTRO_PEA_(—)1_PEA_(—)1_T16 (SEQ ID NO: 278) and HUMOSTRO_PEA_(—)1_PEA_(—)1_T30 (SEQ ID NO: 279). Table 23 below describes the starting and ending position of this segment on each transcript.

TABLE 23 Segment location on transcripts Segment Segment starting ending Transcript name position position HUMOSTRO_PEA_1_PEA_1_T14 211 252 (SEQ ID NO:277) HUMOSTRO_PEA_1_PEA_1_T16 211 252 (SEQ ID NO:278) HUMOSTRO_PEA_1_PEA_1_T30 211 252 (SEQ ID NO:279)

Segment cluster HUMOSTRO_PEA_(—)1_PEA_(—)1_node_(—)7 (SEQ ID NO: 288) according to the present invention is supported by 357 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HUMOSTRO_PEA_(—)1_PEA_(—)1_T14 (SEQ ID NO: 277), HUMOSTRO_PEA_(—)1_PEA_(—)1_T16 (SEQ ID NO: 278) and HUMOSTRO_PEA_(—)1_PEA_(—)1_T30 (SEQ ID NO: 279). Table 24 below describes the starting and ending position of this segment on each transcript.

TABLE 24 Segment location on transcripts Segment Segment starting ending Transcript name position position HUMOSTRO_PEA_1_PEA_1_T14 253 291 (SEQ ID NO:277) HUMOSTRO_PEA_1_PEA_1_T16 253 291 (SEQ ID NO:278) HUMOSTRO_PEA_1_PEA_1_T30 253 291 (SEQ ID NO:279)

Segment cluster HUMOSTRO_PEA_(—)1_PEA_(—)1_node_(—)8 (SEQ ID NO: 289) according to the present invention is supported by 1 library. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HUMOSTRO_PEA_(—)1_PEA_(—)1_T30 (SEQ ID NO: 279). Table 25 below describes the starting and ending position of this segment on each transcript.

TABLE 25 Segment location on transcripts Segment Segment starting ending Transcript name position position HUMOSTRO_PEA_1_PEA_1_T30 292 378 (SEQ ID NO:279)

Segment cluster HUMOSTRO_PEA_(—)1_PEA_(—)1_node_(—)15 (SEQ ID NO: 290) according to the present invention is supported by 366 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HUMOSTRO_PEA_(—)1_PEA_(—)1_T14 (SEQ ID NO: 277) and

HUMOSTRO_PEA_(—)1_PEA_(—)1_T16 (SEQ ID NO: 278). Table 26 below describes the starting and ending position of this segment on each transcript.

TABLE 26 Segment location on transcripts Segment Segment starting ending Transcript name position position HUMOSTRO_PEA_1_PEA_1_T14 292 372 (SEQ ID NO:277) HUMOSTRO_PEA_1_PEA_1_T16 481 561 (SEQ ID NO:278)

Segment cluster HUMOSTRO_PEA_(—)1_PEA_(—)1_node_(—)17 (SEQ ID NO: 291) according to the present invention is supported by 261 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HUMOSTRO_PEA_(—)1_PEA_(—)1_T14 (SEQ ID NO: 277) and HUMOSTRO_PEA_(—)1_PEA_(—)1_T16 (SEQ ID NO: 278). Table 27 below describes the starting and ending position of this segment on each transcript.

TABLE 27 Segment location on transcripts Segment Segment starting ending Transcript name position position HUMOSTRO_PEA_1_PEA_1_T14 639 680 (SEQ ID NO:277) HUMOSTRO_PEA_1_PEA_1_T16 562 603 (SEQ ID NO:278)

Segment cluster HUMOSTRO_PEA_(—)1_PEA_(—)1_node_(—)20 (SEQ ID NO: 292) according to the present invention can be found in the following transcript(s): HUMOSTRO_PEA_(—)1_PEA_(—)1_T14 (SEQ ID NO: 277) and HUMOSTRO_PEA_(—)1_PEA_(—)1_T16 (SEQ ID NO: 278). Table 28 below describes the starting and ending position of this segment on each transcript.

TABLE 28 Segment location on transcripts Segment Segment starting ending Transcript name position position HUMOSTRO_PEA_1_PEA_1_T14 681 688 (SEQ ID NO:277) HUMOSTRO_PEA_1_PEA_1_T16 604 611 (SEQ ID NO:278)

Segment cluster HUMOSTRO_PEA_(—)1_PEA_(—)1_node_(—)21 (SEQ ID NO: 293) according to the present invention is supported by 315 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HUMOSTRO_PEA_(—)1_PEA_(—)1_T14 (SEQ ID NO: 277) and HUMOSTRO_PEA_(—)1_PEA_(—)1_T16 (SEQ ID NO: 278). Table 29 below describes the starting and ending position of this segment on each transcript.

TABLE 29 Segment location on transcripts Segment Segment starting ending Transcript name position position HUMOSTRO_PEA_1_PEA_1_T14 689 738 (SEQ ID NO:277) HUMOSTRO_PEA_1_PEA_1_T16 612 661 (SEQ ID NO:278)

Segment cluster HUMOSTRO_PEA_(—)1_PEA_(—)1_node_(—)22 (SEQ ID NO: 294) according to the present invention is supported by 322 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HUMOSTRO_PEA_(—)1_PEA_(—)1_T14 (SEQ ID NO: 277) and HUMOSTRO_PEA_(—)1_PEA_(—)1_T16 (SEQ ID NO: 278). Table 30 below describes the starting and ending position of this segment on each transcript.

TABLE 30 Segment location on transcripts Segment Segment starting ending Transcript name position position HUMOSTRO_PEA_1_PEA_1_T14 739 803 (SEQ ID NO:277) HUMOSTRO_PEA_1_PEA_1_T16 662 726 (SEQ ID NO:278)

Segment cluster HUMOSTRO_PEA_(—)1_PEA_(—)1_node_(—)24 (SEQ ID NO: 295) according to the present invention is supported by 270 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HUMOSTRO_PEA_(—)1_PEA_(—)1_T14 (SEQ ID NO: 277) and HUMOSTRO_PEA_(—)1_PEA_(—)1_T16 (SEQ ID NO: 278). Table 31 below describes the starting and ending position of this segment on each transcript.

TABLE 31 Segment location on transcripts Segment Segment starting ending Transcript name position position HUMOSTRO_PEA_1_PEA_1_T14 968 1004 (SEQ ID NO:277) HUMOSTRO_PEA_1_PEA_1_T16 891 927 (SEQ ID NO:278)

Segment cluster HUMOSTRO_PEA_(—)1_PEA_(—)1_node_(—)26 (SEQ ID NO: 296) according to the present invention can be found in the following transcript(s): HUMOSTRO_PEA_(—)1_PEA_(—)1_T14 (SEQ ID NO: 277) and HUMOSTRO_PEA_(—)1_PEA_(—)1_T16 (SEQ ID NO: 278). Table 32 below describes the starting and ending position of this segment on each transcript.

TABLE 32 Segment location on transcripts Segment Segment starting ending Transcript name position position HUMOSTRO_PEA_1_PEA_1_T14 1005 1022 (SEQ ID NO:277) HUMOSTRO_PEA_1_PEA_1_T16 928 945 (SEQ ID NO:278)

Segment cluster HUMOSTRO_PEA_(—)1_PEA_(—)1_node_(—)27 (SEQ ID NO: 297) according to the present invention is supported by 260 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HUMOSTRO_PEA_(—)1_PEA_(—)1_T14 (SEQ ID NO: 277) and HUMOSTRO_PEA_(—)1_PEA_(—)1_T16 (SEQ ID NO: 278). Table 33 below describes the starting and ending position of this segment on each transcript.

TABLE 33 Segment location on transcripts Segment Segment starting ending Transcript name position position HUMOSTRO_PEA_1_PEA_1_T14 1023 1048 (SEQ ID NO:277) HUMOSTRO_PEA_1_PEA_1_T16 946 971 (SEQ ID NO:278)

Segment cluster HUMOSTRO_PEA_(—)1_PEA_(—)1_node_(—)28 (SEQ ID NO: 298) according to the present invention is supported by 273 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HUMOSTRO_PEA_(—)1_PEA_(—)1_T14 (SEQ ID NO: 277) and HUMOSTRO_PEA_(—)1_PEA_(—)1_T16 (SEQ ID NO: 278). Table 34 below describes the starting and ending position of this segment on each transcript.

TABLE 34 Segment location on transcripts Segment Segment starting ending Transcript name position position HUMOSTRO_PEA_1_PEA_1_T14 1049 1100 (SEQ ID NO:277) HUMOSTRO_PEA_1_PEA_1_T16 972 1023 (SEQ ID NO:278)

Segment cluster HUMOSTRO_PEA_(—)1_PEA_(—)1_node_(—)29 (SEQ ID NO: 299) according to the present invention is supported by 272 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HUMOSTRO_PEA_(—)1_PEA_(—)1_T14 (SEQ ID NO: 277) and HUMOSTRO_PEA_(—)1_PEA_(—)1_T16 (SEQ ID NO: 278). Table 35 below describes the starting and ending position of this segment on each transcript.

TABLE 35 Segment location on transcripts Segment Segment starting ending Transcript name position position HUMOSTRO_PEA_1_PEA_1_T14 1101 1151 (SEQ ID NO:277) HUMOSTRO_PEA_1_PEA_1_T16 1024 1074 (SEQ ID NO:278)

Segment cluster HUMOSTRO_PEA_(—)1_PEA_(—)1_node_(—)30 (SEQ ID NO. 300) according to the present invention can be found in the following transcript(s): HUMOSTRO_PEA_(—)1_PEA_(—)1_T14 (SEQ ID NO: 277) and HUMOSTRO_PEA_(—)1_PEA_(—)1_T16 (SEQ ID NO: 278). Table 36 below describes the starting and ending position of this segment on each transcript.

TABLE 36 Segment location on transcripts Segment Segment starting ending Transcript name position position HUMOSTRO_PEA_1_PEA_1_T14 1152 1163 (SEQ ID NO:277) HUMOSTRO_PEA_1_PEA_1_T16 1075 1086 (SEQ ID NO:278)

Segment cluster HUMOSTRO_PEA_(—)1_PEA_(—)1_node_(—)32 (SEQ ID NO: 301) according to the present invention is supported by 293 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HUMOSTRO_PEA_(—)1_PEA_(—)1_T14 (SEQ ID NO: 277) and HUMOSTRO_PEA_(—)1_PEA_(—)1_T16 (SEQ ID NO: 278). Table 37 below describes the starting and ending position of this segment on each transcript.

TABLE 37 Segment location on transcripts Segment Segment starting ending Transcript name position position HUMOSTRO_PEA_1_PEA_1_T14 1394 1427 (SEQ ID NO:277) HUMOSTRO_PEA_1_PEA_1_T16 1317 1350 (SEQ ID NO:278)

Segment cluster HUMOSTRO_PEA_(—)1_PEA_(—)1_node_(—)34 (SEQ ID NO: 302) according to the present invention is supported by 301 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HUMOSTRO_PEA_(—)1_PEA_(—)1_T14 (SEQ ID NO: 277) and HUMOSTRO_PEA_(—)1_PEA_(—)1_T16 (SEQ ID NO: 278). Table 38 below describes the starting and ending position of this segment on each transcript.

TABLE 38 Segment location on transcripts Segment Segment starting ending Transcript name position position HUMOSTRO_PEA_1_PEA_1_T14 1428 1468 (SEQ ID NO:277) HUMOSTRO_PEA_1_PEA_1_T16 1351 1391 (SEQ ID NO:278)

Segment cluster HUMOSTRO_PEA_(—)1_PEA_(—)1_node_(—)36 according to the present invention is supported by 292 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HUMOSTRO_PEA_(—)1_PEA_(—)1_T14 (SEQ ID NO: 277) and HUMOSTRO_PEA_(—)1_PEA_(—)1_T16 (SEQ ID NO: 278). Table 39 below describes the starting and ending position of this segment on each transcript.

TABLE 39 Segment location on transcripts Segment Segment starting ending Transcript name position position HUMOSTRO_PEA_1_PEA_1_T14 1469 1504 (SEQ ID NO:277) HUMOSTRO_PEA_1_PEA_1_T16 1392 1427 (SEQ ID NO:278)

Segment cluster HUMOSTRO_PEA_(—)1_PEA_(—)1_node_(—)37 (SEQ ID NO: 304) according to the present invention is supported by 295 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HUMOSTRO_PEA_(—)1_PEA_(—)1_T14 (SEQ ID NO: 277) and HUMOSTRO_PEA_(—)1_PEA_(—)1_T16 (SEQ ID NO: 278). Table 40 below describes the starting and ending position of this segment on each transcript.

TABLE 40 Segment location on transcripts Segment Segment starting ending Transcript name position position HUMOSTRO_PEA_1_PEA_1_T14 1505 1623 (SEQ ID NO:277) HUMOSTRO_PEA_1_PEA_1_T16 1428 1546 (SEQ ID NO:278)

Segment cluster HUMOSTRO_PEA_(—)1_PEA_(—)1_node_(—)38 (SEQ ID NO: 305) according to the present invention can be found in the following transcript(s): HUMOSTRO_PEA_(—)1_PEA_(—)1_T14 (SEQ ID NO: 277) and HUMOSTRO_PEA_(—)1_PEA_(—)1_T16 (SEQ ID NO: 278). Table 41 below describes the starting and ending position of this segment on each transcript.

TABLE 41 Segment location on transcripts Segment Segment starting ending Transcript name position position HUMOSTRO_PEA_1_PEA_1_T14 1624 1634 (SEQ ID NO:277) HUMOSTRO_PEA_1_PEA_1_T16 1547 1557 (SEQ ID NO:278)

Segment cluster HUMOSTRO_PEA_(—)1_PEA_(—)1_node_(—)39 (SEQ ID NO: 306) according to the present invention is supported by 268 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HUMOSTRO_PEA_(—)1_PEA_(—)1_T14 (SEQ ID NO: 277) and HUMOSTRO_PEA_(—)1_PEA_(—)1_T16 (SEQ ID NO: 278). Table 42 below describes the starting and ending position of this segment on each transcript.

TABLE 42 Segment location on transcripts Segment Segment starting ending Transcript name position position HUMOSTRO_PEA_1_PEA_1_T14 1635 1725 (SEQ ID NO:277) HUMOSTRO_PEA_1_PEA_1_T16 1558 1648 (SEQ ID NO:278)

Segment cluster HUMOSTRO_PEA_(—)1_PEA_(—)1_node_(—)40 (SEQ ID NO: 307) according to the present invention can be found in the following transcript(s): HUMOSTRO_PEA_(—)1_PEA_(—)1_T14 (SEQ ID NO: 277) and HUMOSTRO_PEA_(—)1_PEA_(—)1_T16 (SEQ ID NO: 278). Table 43 below describes the starting and ending position of this segment on each transcript.

TABLE 43 Segment location on transcripts Segment Segment starting ending Transcript name position position HUMOSTRO_PEA_1_PEA_1_T14 1726 1743 (SEQ ID NO:277) HUMOSTRO_PEA_1_PEA_1_T16 1649 1666 (SEQ ID NO:278)

Segment cluster HUMOSTRO_PEA_(—)1_PEA_(—)1_node_(—)41 (SEQ ID NO: 308) according to the present invention can be found in the following transcript(s): HUMOSTRO_PEA_(—)1_PEA_(—)1_T14 (SEQ ID NO: 277) and HUMOSTRO_PEA_(—)1_PEA_(—)1_T16 (SEQ ID NO: 278). Table 44 below describes the starting and ending position of this segment on each transcript.

TABLE 44 Segment location on transcripts Segment Segment starting ending Transcript name position position HUMOSTRO_PEA_1_PEA_1_T14 1744 1749 (SEQ ID NO:277) HUMOSTRO_PEA_1_PEA_1_T16 1667 1672 (SEQ ID NO:278)

Segment cluster HUMOSTRO_PEA_(—)1_PEA_(—)1_node_(—)42 (SEQ ID NO: 309) according to the present invention is supported by 224 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HUMOSTRO_PEA_(—)1_PEA_(—)1_T14 (SEQ ID NO: 277) and HUMOSTRO_PEA_(—)1_PEA_(—)1_T16 (SEQ ID NO: 278). Table 45 below describes the starting and ending position of this segment on each transcript.

TABLE 45 Segment location on transcripts Segment Segment starting ending Transcript name position position HUMOSTRO_PEA_1_PEA_1_T14 1750 1809 (SEQ ID NO:277) HUMOSTRO_PEA_1_PEA_1_T16 1673 1732 (SEQ ID NO:278) Variant protein alignment to the previously known protein: Sequence name: OSTP_HUMAN Sequence documentation:

Alignment of: HUMOSTRO_PEA_(—)1_PEA_(—)1_P21 (SEQ ID NO: 311) x OSTP_HUMAN ..

Alignment segment 1/1:

Quality: 578.00 Escore: 0 Matching length: 58 Total length: 58 Matching Percent Similarity: 100.00 Matching Percent Identity: 100.00 Total Percent Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0

Alignment:

Sequence name: OSTP_HUMAN Sequence documentation:

Alignment of: HUMOSTRO_PEA_(—)1_PEA_(—)1_P25 (SEQ ID NO: 312) x OSTP_HUMAN ..

Alignment segment 1/1:

Quality: 301.00 Escore: 0 Matching length: 31 Total length: 31 Matching Percent Similarity: 100.00 Matching Percent Identity: 100.00 Total Percent Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0

Alignment:

Sequence name: OSTP_HUMAN Sequence documentation:

Alignment of: HUMOSTRO_PEA_(—)1_PEA_(—)1_P30 (SEQ ID NO: 313) x OSTP_HUMAN ..

Alignment segment 1/1:

Quality: 301.00 Escore: 0 Matching length: 31 Total length: 31 Matching Percent Similarity: 100.00 Matching Percent Identity: 100.00 Total Percent Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0

Alignment:

Description for Cluster T46984

Cluster T46984 features 21 transcript(s) and 49 segment(s) of interest, the names for which are given in Tables 1 and 2, respectively, the sequences themselves are given at the end of the application. The selected protein variants are given in table 3.

TABLE 1 Transcripts of interest SEQ ID Transcript Name NO: T46984_PEA_1_T2 314 T46984_PEA_1_T3 315 T46984_PEA_1_T12 316 T46984_PEA_1_T13 317 T46984_PEA_1_T14 318 T46984_PEA_1_T15 319 T46984_PEA_1_T19 320 T46984_PEA_1_T23 321 T46984_PEA_1_T27 322 T46984_PEA_1_T32 323 T46984_PEA_1_T34 324 T46984_PEA_1_T35 325 T46984_PEA_1_T40 326 T46984_PEA_1_T42 327 T46984_PEA_1_T43 328 T46984_PEA_1_T46 329 T46984_PEA_1_T47 330 T46984_PEA_1_T48 331 T46984_PEA_1_T51 332 (SEQ ID NO:332) T46984_PEA_1_T52 333 T46984_PEA_1_T54 334

TABLE 2 Segments of interest SEQ ID Segment Name NO: T46984_PEA_1_node_2 335 T46984_PEA_1_node_4 336 T46984_PEA_1_node_6 337 T46984_PEA_1_node_12 338 T46984_PEA_1_node_14 339 T46984_PEA_1_node_25 340 T46984_PEA_1_node_29 341 T46984_PEA_1_node_34 342 T46984_PEA_1_node_46 343 T46984_PEA_1_node_47 344 T46984_PEA_1_node_52 345 T46984_PEA_1_node_65 346 T46984_PEA_1_node_69 347 T46984_PEA_1_node_75 348 T46984_PEA_1_node_86 349 T46984_PEA_1_node_9 350 T46984_PEA_1_node_13 351 T46984_PEA_1_node_19 352 T46984_PEA_1_node_21 353 T46984_PEA_1_node_22 354 T46984_PEA_1_node_26 355 T46984_PEA_1_node_28 356 T46984_PEA_1_node_31 357 T46984_PEA_1_node_32 358 T46984_PEA_1_node_38 359 T46984_PEA_1_node_39 360 T46984_PEA_1_node_40 361 T46984_PEA_1_node_42 362 T46984_PEA_1_node_43 363 T46984_PEA_1_node_48 364 T46984_PEA_1_node_49 365 T46984_PEA_1_node_50 366 T46984_PEA_1_node_51 367 T46984_PEA_1_node_53 368 T46984_PEA_1_node_54 369 T46984_PEA_1_node_55 370 T46984_PEA_1_node_57 371 T46984_PEA_1_node_60 372 T46984_PEA_1_node_62 373 T46984_PEA_1_node_66 374 T46984_PEA_1_node_67 375 T46984_PEA_1_node_70 376 T46984_PEA_1_node_71 377 T46984_PEA_1_node_72 378 T46984_PEA_1_node_73 379 T46984_PEA_1_node_74 380 T46984_PEA_1_node_83 381 T46984_PEA_1_node_84 382 T46984_PEA_1_node_85 383

TABLE 3 Proteins of interest SEQ ID Corresponding Protein Name NO: Transcript(s) T46984_PEA_1_P2 385 T46984_PEA_1_T2 (SEQ ID NO:314) (SEQ ID NO:314); T46984_PEA_1_T12 (SEQ ID NO:316); T46984 PEA_1_T23 (SEQ ID NO:321) T46984_PEA_1_P3 386 T46984_PEA_1_T3 (SEQ ID NO:315); T46984_PEA_1_T19 (SEQ ID NO:320) T46984_PEA_1_P10 387 T46984_PEA_1_T13 (SEQ ID NO:317) T46984_PEA_1_P11 388 T46984 PEA_1_T14 (SEQ ID NO:318) T46984_PEA_1_P12 389 T46984_PEA_1_T15 (SEQ ID NO:319) T46984_PEA_1_P21 390 T46984_PEA_1_T27 (SEQ ID NO:322) T46984_PEA_1_P27 391 T46984_PEA_1_T34 (SEQ ID NO:324) T46984_PEA_1_P32 392 T46984_PEA_1_T40 (SEQ ID NO:326) T46984_PEA_1_P34 393 T46984_PEA_1_T42 (SEQ ID NO:327) T46984_PEA_1_P35 394 T46984_PEA_1_T43 (SEQ ID NO:328) T46984_PEA_1_P38 395 T46984_PEA_1_T47 (SEQ ID NO:330) T46984_PEA_1_P39 396 T46984_PEA_1_T48 (SEQ ID NO:331) T46984_PEA_1_P45 397 T46984_PEA_1_T32 (SEQ ID NO:323) T46984_PEA_1_P46 398 T46984_PEA_1_T35 (SEQ ID NO:325)

These sequences are variants of the known protein Dolichyl-diphosphooligosaccharide—protein glycosyltransferase 63 kDa subunit precursor (SwissProt accession identifier RIB2_HUMAN; known also according to the synonyms EC 2.4.1.119; Ribophorin II; RPN-II; RIBIIR), SEQ ID NO: 384, referred to herein as the previously known protein.

Protein Dolichyl-diphosphooligosaccharide—protein glycosyltransferase 63 kDa subunit precursor is known or believed to have the following function(s): Essential subunit of N-oligosaccharyl transferase enzyme which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains. The sequence for protein Dolichyl-diphosphooligosaccharide—protein glycosyltransferase 63 kDa subunit precursor is given at the end of the application, as “Dolichyl-diphosphooligosaccharide—protein glycosyltransferase 63 kDa subunit precursor amino acid sequence”. Known polymorphisms for this sequence are as shown in Table 4.

TABLE 4 Amino acid mutations for Known Protein SNP position(s) on amino acid sequence Comment 197 V -> L 201 F -> C 260 A -> S 423 V -> M

Protein Dolichyl-diphosphooligosaccharide—protein glycosyltransferase 63 kDa subunit precursor localization is believed to be Type I membrane protein. Endoplasmic reticulum.

The following GO Annotation(s) apply to the previously known protein. The following annotation(s) were found: protein modification, which are annotation(s) related to Biological Process; oligosaccharyl transferase; dolichyl-diphosphooligosaccharide-protein glycosyltransferase; transferase, which are annotation(s) related to Molecular Function; and oligosaccharyl transferase complex; integral membrane protein, which are annotation(s) related to Cellular Component.

The GO assignment relies on information from one or more of the SwissProt/TremBl Protein knowledgebase, available from <http://www.expasy.ch/sprot/>; or Locuslink, available from <http://www.ncbi.nlm.nih.gov/projects/LocusLink/>.

Cluster T46984 can be used as a diagnostic marker according to overexpression of transcripts of this cluster in cancer. Expression of such transcripts in normal tissues is also given according to the previously described methods. The term “number” in the left hand column of the table and the numbers on the y-axis of FIG. 39 refer to weighted expression of ESTs in each category, as “parts per million” (ratio of the expression of ESTs for a particular cluster to the expression of all ESTs in that category, according to parts per million).

Overall, the following results were obtained as shown with regard to the histograms in FIG. 39 and Table 5. This cluster is overexpressed (at least at a minimum level) in the following pathological conditions: epithelial malignant tumors, a mixture of malignant tumors from different tissues, breast malignant tumors, ovarian carcinoma and pancreas carcinoma.

TABLE 5 Normal tissue distribution Name of Tissue Number adrenal 240 bladder 287 Bone 592 Brain 145 Colon 157 epithelial 144 general 163 head and neck 50 Kidney 139 Liver 156 Lung 155 Lymph nodes 194 Breast 105 bone marrow 62 Muscle 62 Ovary 0 pancreas 72 prostate 201 Skin 91 stomach 219 T cells 0 Thyroid 0 Uterus 200

TABLE 6 P values and ratios for expression in cancerous tissue Name of Tissue P1 P2 SP1 R3 SP2 R4 adrenal 6.3e−01 5.4e−01 6.2e−01 0.8 2.5e−01 1.0 bladder 5.4e−01 5.9e−01 3.0e−01 1.0 6.5e−01 0.7 Bone 3.9e−01 3.7e−01 9.8e−01 0.4 9.9e−01 0.4 Brain 3.3e−01 2.9e−01 1.4e−01 1.2 2.0e−01 1.0 Colon 8.6e−02 5.9e−02 2.6e−01 1.3 2.1e−03 1.4 epithelial 5.3e−05 6.2e−07 2.8e−08 1.9 3.4e−21 2.4 general 1.0e−04 7.3e−08 9.3e−12 1.7 8.0e−33 2.0 head and neck 4.5e−01 5.4e−01 1 0.8 7.5e−01 0.9 Kidney 6.6e−01 6.5e−01 3.2e−01 1.2 5.3e−02 1.5 Liver 5.5e−01 5.6e−01 6.5e−01 1.0 1.2e−01 1.4 Lung 3.0e−01 1.7e−01 1.5e−01 1.4 6.0e−02 1.4 Lymph nodes 2.9e−01 5.5e−01 2.9e−01 0.8 4.3e−01 1.0 Breast 2.4e−02 5.8e−03 3.7e−02 2.2 1.7e−04 2.7 bone marrow 7.1e−01 7.5e−01 1 0.3 1.2e−02 1.8 Muscle 5.0e−01 3.7e−01 4.7e−01 1.5 2.1e−08 1.3 Ovary 1.6e−02 7.0e−03 1.5e−02 6.1 4.8e−06 7.1 pancreas 1.4e−01 5.4e−02 2.2e−05 2.9 2.4e−07 3.9 prostate 3.4e−01 1.9e−01 2.2e−01 1.2 1.4e−01 1.3 Skin 3.7e−01 1.5e−01 4.2e−02 2.4 1.1e−04 1.9 stomach 6.1e−01 1.4e−01 7.3e−01 0.4 6.1e−02 1.6 T cells 1 6.7e−01 1 1.0 5.2e−01 1.8 Thyroid 4.8e−02 4.8e−02 2.0e−01 3.4 2.0e−01 3.4 Uterus 2.3e−01 1.3e−01 2.2e−02 1.5 5.0e−02 1.4

As noted above, cluster T46984 features 21 transcript(s), which were listed in Table 1 above. These transcript(s) encode for protein(s) which are variant(s) of protein Dolichyl-diphosphooligosaccharide—protein glycosyltransferase 63 kDa subunit precursor. A description of each variant protein according to the present invention is now provided.

Variant protein T46984_PEA_(—)1_P2 (SEQ ID NO: 385) according to the present invention has an amino acid sequence as given at the end of the application; it is encoded by transcript(s) T46984_PEA_(—)1_T2 (SEQ ID NO: 314) (SEQ ID NO: 314). An alignment is given to the known protein (Dolichyl-diphosphooligosaccharide—protein glycosyltransferase 63 kDa subunit precursor) at the end of the application. One or more alignments to one or more previously published protein sequences are given at the end of the application. A brief description of the relationship of the variant protein according to the present invention to each such aligned protein is as follows:

Comparison report between T46984_PEA_(—)1_P2 (SEQ ID NO: 385) and RIB2_HUMAN:

1. An isolated chimeric polypeptide encoding for T46984_PEA_(—)1_P2 (SEQ ID NO: 385), comprising a first amino acid sequence being at least 90% homologous to MAPPGSSTVFLLALTIIASTWALTPTHYLTKHDVERLKASLDRPFTNLESAFYSIVGLSSL GAQVPDAKKACTYIRSNLDPSNVDSLFYAAQASQALSGCEISISNETKDLLLAAVSEDSS VTQIYHAVAALSGFGLPLASQEALSALTARLSKEETVLATVQALQTASHLSQQADLRSI VEEIEDLVARLDELGGVYLQFEEGLETTALFVAATYKLMDHVGTEPSIKEDQVIQLMNA IFSKKNFESLSEAFSVASAAAVLSHNRYHVPVVVVPEGSASDTHEQAILRLQVTNVLSQ PLTQATVKLEHAKSVASRATVLQKTSFTPVGDVFELNFMNVKFSSGYYDFLVEVEGDN RYIANTVELRVKISTEVGITNVDLSTVDKDQSIAPKTTRVTYPAKAKGTFIADSHQNFAL FFQLVDVNTGAELTPHQTFVRLHNQKTGQEVVFVAEPDNKNVYKFELDTSERKIEFDS ASGTYTLYLIIGDATLKNPILWNV corresponding to amino acids 1-498 of RIB2_HUMAN, which also corresponds to amino acids 1-498 of T46984_PEA_(—)1_P2 (SEQ ID NO: 385), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence VCA corresponding to amino acids 499-501 of T46984_PEA_(—)1_P2 (SEQ ID NO: 385), wherein said first amino acid sequence and second amino acid sequence are contiguous and in a sequential order.

The location of the variant protein was determined according to results from a number of different software programs and analyses, including analyses from SignalP and other specialized programs. The variant protein is believed to be located as follows with regard to the cell: secreted. The protein localization is believed to be secreted because both signal-peptide prediction programs predict that this protein has a signal peptide, and neither trans-membrane region prediction program predicts that this protein has a trans-membrane region.

The glycosylation sites of variant protein T46984_PEA_(—)1_P2 (SEQ ID NO: 385), as compared to the known protein Dolichyl-diphosphooligosaccharide—protein glycosyltransferase 63 kDa subunit precursor, are described in Table 7 (given according to their position(s) on the amino acid sequence in the first column; the second column indicates whether the glycosylation site is present in the variant protein; and the last column indicates whether the position is different on the variant protein).

TABLE 7 Glycosylation site(s) Position(s) on known Present in variant amino acid sequence protein? Position in variant protein? 106 yes 106

Variant protein T46984_PEA_(—)1_P2 (SEQ ID NO: 385) is encoded by the following transcript(s): T46984_PEA_(—)1_T2 (SEQ ID NO: 314) (SEQ ID NO: 314), for which the sequence(s) is/are given at the end of the application. The coding portion of transcript T46984_PEA_(—)1_T2 (SEQ ID NO: 314) (SEQ ID NO: 314) is shown in bold; this coding portion starts at position 316 and ends at position 1818. The transcript also has the following SNPs as listed in Table 8 (given according to their position on the nucleotide sequence, with the alternative nucleic acid listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein T46984_PEA_(—)1_P2 (SEQ ID NO: 385) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 8 Nucleic acid SNPs SNP position on nucleotide sequence Alternative nucleic acid Previously known SNP? 28 G -> C No 173 G -> C Yes 256 C -> T Yes 274 G -> C Yes 325 C -> No 389 C -> G Yes 610 G -> A Yes 718 T -> No 724 C -> No 844 C -> T Yes 857 -> G No 885 C -> No 897 -> G No 1002 G -> A No 1048 A -> No 1048 A -> G No 1068 A -> C No 1076 G -> A Yes 1187 A -> No 1187 A -> C No 1220 A -> G No 1220 A -> T No 1254 T -> G No 1291 A -> C No 1293 C -> G No 1303 G -> A No 1376 G -> T Yes 1588 A -> C No 1618 T -> No 1618 T -> C No 1660 T -> No 1693 A -> C No 1693 A -> T No 2099 G -> A Yes 2124 C -> G Yes 2124 C -> T Yes 2133 A -> G Yes 2501 C -> T Yes 2617 G -> T Yes 2683 C -> T Yes 2741 G -> A Yes 2940 T -> No 3024 G -> A Yes 3158 C -> No 3158 C -> A No 3165 C -> No 3169 G -> No 3354 C -> A No 3374 T -> C Yes 3468 C -> T No 3501 A -> C No 3513 A -> T No 3528 G -> A Yes 3534 -> A No 3543 A -> G No 3568 T -> G No 3582 T -> A No 3582 T -> G No 3682 -> C No 3691 T -> No 3750 A -> C No

Variant protein T46984_PEA_(—)1_P3 (SEQ ID NO: 386) according to the present invention has an amino acid sequence as given at the end of the application; it is encoded by transcript(s) T46984_PEA_(—)1_T3 (SEQ ID NO: 315). An alignment is given to the known protein (Dolichyl-diphosphooligosaccharide—protein glycosyltransferase 63 kDa subunit precursor) at the end of the application. One or more alignments to one or more previously published protein sequences are given at the end of the application. A brief description of the relationship of the variant protein according to the present invention to each such aligned protein is as follows:

Comparison report between T46984_PEA_(—)1_P3 (SEQ ID NO: 386) and RIB2_HUMAN:

1. An isolated chimeric polypeptide encoding for T46984_PEA_(—)1_P3 (SEQ ID NO: 386), comprising a first amino acid sequence being at least 90% homologous to MAPPGSSTVFLLALTIIASTWALTPTHYLTKHDVERLKASLDRPFTNLESAFYSIVGLSSL GAQVPDAKKACTYIRSNLDPSNVDSLFYAAQASQALSGCEISISNETKDLLLAAVSEDSS VTQIYHAVAALSGFGLPLASQEALSALTARLSKEETVLATVQALQTASHLSQQADLRSI VEEIEDLVARLDELGGVYLQFEEGLETTALFVAATYKLMDHVGTEPSIKEDQVIQLMNA IFSKKNFESLSEAFSVASAAAVLSHNRYHVPVVVVPEGSASDTHEQAILRLQVTNVLSQ PLTQATVKLEHAKSVASRATVLQKTSFTPVGDVFELNFMNVKFSSGYYDFLVEVEGDN RYIANTVELRVKISTEVGITNVDLSTVDKDQSIAPKTTRVTYPAKAKGTFIADSHQNFAL FFQLVDVNTGAELTPHQ corresponding to amino acids 1-433 of RIB2_HUMAN, which also corresponds to amino acids 1-433 of T46984_PEA_(—)1_P3 (SEQ ID NO: 386), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence ICHIWKLIFLP (SEQ ID NO: 1061) corresponding to amino acids 434-444 of T46984_PEA_(—)1_P3 (SEQ ID NO: 386), wherein said first amino acid sequence and second amino acid sequence are contiguous and in a sequential order.

2. An isolated polypeptide encoding for a tail of T46984_PEA_(—)1_P3 (SEQ ID NO: 386), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence ICHIWKLIFLP (SEQ ID NO: 1061) in T46984_PEA_(—)1_P3 (SEQ ID NO: 386).

The location of the variant protein was determined according to results from a number of different software programs and analyses, including analyses from SignalP and other specialized programs. The variant protein is believed to be located as follows with regard to the cell: secreted. The protein localization is believed to be secreted because both signal-peptide prediction programs predict that this protein has a signal peptide, and neither trans-membrane region prediction program predicts that this protein has a trans-membrane region.

Variant protein T46984_PEA_(—)1_P3 (SEQ ID NO: 386) also has the following non-silent SNPs (Single Nucleotide Polymorphisms) as listed in Table 9, (given according to their position(s) on the amino acid sequence, with the alternative amino acid(s) listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein T46984_PEA_(—)1_P3 (SEQ ID NO: 386) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 9 Amino acid mutations SNP position(s) on amino acid sequence Alternative amino acid(s) Previously known SNP? 4 P -> No 25 P -> R Yes 99 G -> R Yes 135 F -> No 137 L -> No 190 R -> No 245 N -> No 245 N -> D No 251 E -> D No 254 S -> N Yes 291 Q -> No 291 Q -> P No 302 Q -> R No 302 Q -> L No 326 T -> P No 330 D -> N No 354 G -> V Yes 425 T -> P No

The glycosylation sites of variant protein T46984_PEA_(—)1_P3 (SEQ ID NO: 386), as compared to the known protein Dolichyl-diphosphooligosaccharide—protein glycosyltransferase 63 kDa subunit precursor, are described in Table 10 (given according to their position(s) on the amino acid sequence in the first column; the second column indicates whether the glycosylation site is present in the variant protein; and the last column indicates whether the position is different on the variant protein).

TABLE 10 Glycosylation site(s) Position(s) on known Present Position in amino acid sequence in variant protein? variant protein? 106 yes 106

Variant protein T46984_PEA_(—)1_P3 (SEQ ID NO: 386) is encoded by the following transcript(s): T46984_PEA_(—)1_T3 (SEQ ID NO: 315), for which the sequence(s) is/are given at the end of the application. The coding portion of transcript T46984_PEA_(—)1_T3 (SEQ ID NO: 315) is shown in bold; this coding portion starts at position 316 and ends at position 1647. The transcript also has the following SNPs as listed in Table 11 (given according to their position on the nucleotide sequence, with the alternative nucleic acid listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein T46984_PEA_(—)1_P3 (SEQ ID NO: 386) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 11 Nucleic acid SNPs SNP position on nucleotide sequence Alternative nucleic acid Previously known SNP? 28 G -> C No 173 G -> C Yes 256 C -> T Yes 274 G -> C Yes 325 C -> No 389 C -> G Yes 610 G -> A Yes 718 T -> No 724 C -> No 844 C -> T Yes 857 -> G No 885 C -> No 897 -> G No 1002 G -> A No 1048 A -> No 1048 A -> G No 1068 A -> C No 1076 G -> A Yes 1187 A -> No 1187 A -> C No 1220 A -> G No 1220 A -> T No 1254 T -> G No 1291 A -> C No 1293 C -> G No 1303 G -> A No 1376 G -> T Yes 1588 A -> C No 1784 C -> T Yes 1959 G -> A Yes 2112 G -> A Yes 2137 C -> G Yes 2246 T -> No 2246 T -> C No 2288 T -> No 2321 A -> C No 2321 A -> T No 2552 C -> No 2552 C -> A No 2559 C -> No 2563 G -> No 2748 C -> A No 2768 T -> C Yes 2862 C -> T No 2895 A -> C No 2907 A -> T No 2922 G -> A Yes 2928 -> A No 2937 A -> G No 2962 T -> G No 2976 T -> A No 2976 T -> G No 3076 -> C No 3085 T -> No 3144 A -> C No

Variant protein T46984_PEA_(—)1_P10 (SEQ ID NO: 387) according to the present invention has an amino acid sequence as given at the end of the application; it is encoded by transcript(s) T46984_PEA_(—)1_T13 (SEQ ID NO: 317). An alignment is given to the known protein (Dolichyl-diphosphooligosaccharide—protein glycosyltransferase 63 kDa subunit precursor) at the end of the application. One or more alignments to one or more previously published protein sequences are given at the end of the application. A brief description of the relationship of the variant protein according to the present invention to each such aligned protein is as follows:

Comparison report between T46984_PEA_(—)1_P10 (SEQ ID NO: 387) and RIB2_HUMAN:

1. An isolated chimeric polypeptide encoding for T46984_PEA_(—)1_P10 (SEQ ID NO: 387), comprising a first amino acid sequence being at least 90% homologous to MAPPGSSTVFLLALTIIASTWALTPTHYLTKHDVERLKASLDRPFTNLESAFYSIVGLSSL GAQVPDAKKACTYIRSNLDPSNVDSLFYAAQASQALSGCEISISNETKDLLLAAVSEDSS VTQIYHAVAALSGFGLPLASQEALSALTARLSKEETVLATVQALQTASHLSQQADLRSI VEEIEDLVARLDELGGVYLQFEEGLETTALFVAATYKLMDHVGTEPSIKEDQVIQLMNA IFSKKNFESLSEAFSVASAAAVLSHNRYHVPVVVVPEGSASDTHEQAILRLQVTNVLSQ PLTQATVKLEHAKSVASRATVLQKTSFTPVGDVFELNFMNVKFSSGYYDFLVEVEGDN RYIANTVELRVKISTEVGITNVDLSTVDKDQSIAPKTTRVTYPAKAKGTFIADSHQNFAL FFQLVDVNTGAELTPHQTFVRLHNQKTGQEVVFVAEPDNKNVYKFELDTSERKIEFDS ASGTYTLYLIIGDATLKNPILWNV corresponding to amino acids 1-498 of RIB2_HUMAN, which also corresponds to amino acids 1-498 of T46984_PEA_(—)1_P10 (SEQ ID NO: 387), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence LMDQK (SEQ ID NO: 1062) corresponding to amino acids 499-503 of T46984_PEA_(—)1_P10 (SEQ ID NO: 387), wherein said first amino acid sequence and second amino acid sequence are contiguous and in a sequential order.

2. An isolated polypeptide encoding for a tail of T46984_PEA_(—)1_P10 (SEQ ID NO: 387), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence LMDQK (SEQ ID NO: 1062) in T46984_PEA_(—)1_P10 (SEQ ID NO: 387).

The location of the variant protein was determined according to results from a number of different software programs and analyses, including analyses from SignalP and other specialized programs. The variant protein is believed to be located as follows with regard to the cell: secreted. The protein localization is believed to be secreted because both signal-peptide prediction programs predict that this protein has a signal peptide, and neither trans-membrane region prediction program predicts that this protein has a trans-membrane region.

Variant protein T46984_PEA_(—)1_P10 (SEQ ID NO: 387) also has the following non-silent SNPs (Single Nucleotide Polymorphisms) as listed in Table 12, (given according to their position(s) on the amino acid sequence, with the alternative amino acid(s) listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein T46984_PEA_(—)1_P10 (SEQ ID NO: 387) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 12 Amino acid mutations SNP position(s) on amino acid Alternative Previously known sequence amino acid(s) SNP? 4 P -> No 25 P -> R Yes 99 G -> R Yes 135 F -> No 137 L -> No 190 R -> No 245 N -> No 245 N -> D No 251 E -> D No 254 S -> N Yes 291 Q -> No 291 Q -> P No 302 Q -> R No 302 Q -> L No 326 T -> P No 330 D -> N No 354 G -> V Yes 425 T -> P No 435 F -> No 435 F -> L No 449 F -> No 460 K -> * No 460 K -> Q No

The glycosylation sites of variant protein T46984_PEA_(—)1_P10 (SEQ ID NO: 387), as compared to the known protein Dolichyl-diphosphooligosaccharide—protein glycosyltransferase 63 kDa subunit precursor, are described in Table 13 (given according to their position(s) on the amino acid sequence in the first column; the second column indicates whether the glycosylation site is present in the variant protein; and the last column indicates whether the position is different on the variant protein).

TABLE 13 Glycosylation site(s) Position(s) on known Present Position in amino acid sequence in variant protein? variant protein? 106 yes 106

Variant protein T46984_PEA_(—)1_P10 (SEQ ID NO: 387) is encoded by the following transcript(s): T46984_PEA_(—)1_T13 (SEQ ID NO: 317), for which the sequence(s) is/are given at the end of the application. The coding portion of transcript T46984_PEA_(—)1_T13 (SEQ ID NO: 317) is shown in bold; this coding portion starts at position 316 and ends at position 1824. The transcript also has the following SNPs as listed in Table 14 (given according to their position on the nucleotide sequence, with the alternative nucleic acid listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein T46984_PEA_(—)1_P10 (SEQ ID NO: 387) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 14 Nucleic acid SNPs SNP position on nucleotide sequence Alternative nucleic acid Previously known SNP? 28 G -> C No 173 G -> C Yes 256 C -> T Yes 274 G -> C Yes 325 C -> No 389 C -> G Yes 610 G -> A Yes 718 T -> No 724 C -> No 844 C -> T Yes 857 -> G No 885 C -> No 897 -> G No 1002 G -> A No 1048 A -> No 1048 A -> G No 1068 A -> C No 1076 G -> A Yes 1187 A -> No 1187 A -> C No 1220 A -> G No 1220 A -> T No 1254 T -> G No 1291 A -> C No 1293 C -> G No 1303 G -> A No 1376 G -> T Yes 1588 A -> C No 1618 T -> No 1618 T -> C No 1660 T -> No 1693 A -> C No 1693 A -> T No 1845 T -> No 1983 C -> No 1983 C -> A No 1990 C -> No 1994 G -> No 2179 C -> A No 2199 T -> C Yes 2293 C -> T No 2326 A -> C No 2338 A -> T No 2353 G -> A Yes 2359 -> A No 2368 A -> G No 2393 T -> G No 2407 T -> A No 2407 T -> G No 2507 -> C No 2516 T -> No 2575 A -> C No

Variant protein T46984_PEA_(—)1_P11 (SEQ ID NO: 388) according to the present invention has an amino acid sequence as given at the end of the application; it is encoded by transcript(s) T46984_PEA_(—)1_T14 (SEQ ID NO: 318). An alignment is given to the known protein (Dolichyl-diphosphooligosaccharide—protein glycosyltransferase 63 kDa subunit precursor) at the end of the application. One or more alignments to one or more previously published protein sequences are given at the end of the application. A brief description of the relationship of the variant protein according to the present invention to each such aligned protein is as follows:

Comparison report between T46984_PEA_(—)1_P11 (SEQ ID NO: 388) and RIB2_HUMAN:

1. An isolated chimeric polypeptide encoding for T46984_PEA_(—)1_P11 (SEQ ID NO: 388), comprising a first amino acid sequence being at least 90% homologous to MAPPGSSTVFLLALTIIASTWALTPTHYLTKHDVERLKASLDRPFTNLESAFYSIVGLSSL GAQVPDAKKACTYIRSNLDPSNVDSLFYAAQASQALSGCEISISNETKDLLLAAVSEDSS VTQIYHAVAALSGFGLPLASQEALSALTARLSKEETVLATVQALQTASHLSQQADLRSI VEEIEDLVARLDELGGVYLQFEEGLETTALFVAATYKLMDHVGTEPSIKEDQVIQLMNA IFSKKNFESLSEAFSVASAAAVLSHNRYHVPVVVVPEGSASDTHEQAILRLQVTNVLSQ PLTQATVKLEHAKSVASRATVLQKTSFTPVGDVFELNFMNVKFSSGYYDFLVEVEGDN RYIANTVELRVKISTEVGITNVDLSTVDKDQSIAPKTTRVTYPAKAKGTFIADSHQNFAL FFQLVDVNTGAELTPHQTFVRLHNQKTGQEVVFVAEPDNKNVYKFELDTSERKIEFDS ASGTYTLYLIIGDATLKNPILWNVADVVIKFPEEEAPSTVLSQNLFTPKQEIQHLFREPEK RPPTVVSNTFTALILSPLLLLFALWIRIGANVSNFTFAPSTIIFHLGHAAMLGLMYVYWT QLNMFQTLKYLAILGSVTFLAGNRMLAQQAVKR corresponding to amino acids 1-628 of RIB2_HUMAN, which also corresponds to amino acids 1-628 of T46984_PEA_(—)1_P11 (SEQ ID NO: 388).

The location of the variant protein was determined according to results from a number of different software programs and analyses, including analyses from SignalP and other specialized programs. The variant protein is believed to be located as follows with regard to the cell: membrane. The protein localization is believed to be membrane because although both signal-peptide prediction programs agree that this protein has a signal peptide, both trans-membrane region prediction programs predict that this protein has a trans-membrane region downstream of this signal peptide.

Variant protein T46984_PEA_(—)1_P11 (SEQ ID NO: 388) also has the following non-silent SNPs (Single Nucleotide Polymorphisms) as listed in Table 15, (given according to their position(s) on the amino acid sequence, with the alternative amino acid(s) listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein T46984_PEA_(—)1_P11 (SEQ ID NO: 388) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 15 Amino acid mutations SNP position(s) on amino acid sequence Alternative amino acid(s) Previously known SNP? 4 P -> No 25 P -> R Yes 99 G -> R Yes 135 F -> No 137 L -> No 190 R -> No 245 N -> No 245 N -> D No 251 E -> D No 254 S -> N Yes 291 Q -> P No 291 Q -> No 302 Q -> L No 302 Q -> R No 326 T -> P No 330 D -> N No 354 G -> V Yes 425 T -> P No 435 F -> No 435 F -> L No 449 F -> No 460 K -> Q No 460 K -> * No 537 P -> T No 537 P -> No 539 T -> No 540 V -> No 602 T -> N No

The glycosylation sites of variant protein T46984_PEA_(—)1_P11 (SEQ ID NO: 388), as compared to the known protein Dolichyl-diphosphooligosaccharide—protein glycosyltransferase 63 kDa subunit precursor, are described in Table 16 (given according to their position(s) on the amino acid sequence in the first column; the second column indicates whether the glycosylation site is present in the variant protein; and the last column indicates whether the position is different on the variant protein).

TABLE 16 Glycosylation site(s) Position(s) on known amino Present in Position in acid sequence variant protein? variant protein? 106 yes 106

Variant protein T46984_PEA_(—)1_P11 (SEQ ID NO: 388) is encoded by the following transcript(s): T46984_PEA_(—)1_T14 (SEQ ID NO: 318), for which the sequence(s) is/are given at the end of the application. The coding portion of transcript T46984_PEA_(—)1_T14 (SEQ ID NO: 318) is shown in bold; this coding portion starts at position 316 and ends at position 2199. The transcript also has the following SNPs as listed in Table 17 (given according to their position on the nucleotide sequence, with the alternative nucleic acid listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein T46984_PEA_(—)1_P11 (SEQ ID NO: 388) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 17 Nucleic acid SNPs SNP position on nucleotide Alternative Previously sequence nucleic acid known SNP? 28 G -> C No 173 G -> C Yes 256 C -> T Yes 274 G -> C Yes 325 C -> No 389 C -> G Yes 610 G -> A Yes 718 T -> No 724 C -> No 844 C -> T Yes 857 -> G No 885 C -> No 897 -> G No 1002 G -> A No 1048 A -> No 1048 A -> G No 1068 A -> C No 1076 G -> A Yes 1187 A -> No 1187 A -> C No 1220 A -> G No 1220 A -> T No 1254 T -> G No 1291 A -> C No 1293 C -> G No 1303 G -> A No 1376 G -> T Yes 1588 A -> C No 1618 T -> No 1618 T -> C No 1660 T -> No 1693 A -> C No 1693 A -> T No 1924 C -> No 1924 C -> A No 1931 C -> No 1935 G -> No 2120 C -> A No 2140 T -> C Yes 2449 A -> Yes 2537 C -> T Yes 2614 C -> T Yes 2699 C -> T Yes 2857 G -> A Yes 2879 A -> G Yes 3078 A -> G Yes 3354 G -> A Yes

Variant protein T46984_PEA_(—)1_P12 (SEQ ID NO: 389) according to the present invention has an amino acid sequence as given at the end of the application; it is encoded by transcript(s) T46984_PEA_(—)1_T15 (SEQ ID NO: 319). An alignment is given to the known protein (Dolichyl-diphosphooligosaccharide—protein glycosyltransferase 63 kDa subunit precursor) at the end of the application. One or more alignments to one or more previously published protein sequences are given at the end of the application. A brief description of the relationship of the variant protein according to the present invention to each such aligned protein is as follows:

Comparison report between T46984_PEA_(—)1_P12 (SEQ ID NO: 389) and RIB2_HUMAN:

1. An isolated chimeric polypeptide encoding for T46984_PEA_(—)1_P12 (SEQ ID NO: 389), comprising a first amino acid sequence being at least 90% homologous to MAPPGSSTVFLLALTIIASTWALTPTHYLTKHDVERLKASLDRPFTNLESAFYSIVGLSSL GAQVPDAKKACTYIRSNLDPSNVDSLFYAAQASQALSGCEISISNETKDLLLAAVSEDSS VTQIYHAVAALSGFGLPLASQEALSALTARLSKEETVLATVQALQTASHLSQQADLRSI VEEIEDLVARLDELGGVYLQFEEGLETTALFVAATYKLMDHVGTEPSIKEDQVIQLMNA IFSKKNFESLSEAFSVASAAAVLSHNRYHVPVVVVPEGSASDTHEQAILRLQVTNVLSQ PLTQATVKLEHAKSVASRATVLQKTSFTPVGDVFELNFMN corresponding to amino acids 1-338 of RIB2_HUMAN, which also corresponds to amino acids 1-338 of T46984_PEA_(—)1_P12 (SEQ ID NO: 389), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence SQDLH (SEQ ID NO: 1063) corresponding to amino acids 339-343 of T46984_PEA_(—)1_P12 (SEQ ID NO: 389), wherein said first amino acid sequence and second amino acid sequence are contiguous and in a sequential order.

2. An isolated polypeptide encoding for a tail of T46984_PEA_(—)1_P12 (SEQ ID NO: 389), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence SQDLH (SEQ ID NO: 1063) in T46984_PEA_(—)1_P12 (SEQ ID NO: 389).

The location of the variant protein was determined according to results from a number of different software programs and analyses, including analyses from SignalP and other specialized programs. The variant protein is believed to be located as follows with regard to the cell: secreted. The protein localization is believed to be secreted because both signal-peptide prediction programs predict that this protein has a signal peptide, and neither trans-membrane region prediction program predicts that this protein has a trans-membrane region.

Variant protein T46984_PEA_(—)1_P12 (SEQ ID NO: 389) also has the following non-silent SNPs (Single Nucleotide Polymorphisms) as listed in Table 18, (given according to their position(s) on the amino acid sequence, with the alternative amino acid(s) listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein T46984_PEA_(—)1_P12 (SEQ ID NO: 389) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 18 Amino acid mutations SNP position(s) on amino acid Alternative Previously sequence amino acid(s) known SNP? 4 P -> No 25 P -> R Yes 99 G -> R Yes 135 F -> No 137 L -> No 190 R -> No 245 N -> No 245 N -> D No 251 E -> D No 254 S -> N Yes 291 Q -> No 291 Q -> P No 302 Q -> L No 302 Q -> R No 326 T -> P No 330 D -> N No

The glycosylation sites of variant protein T46984_PEA_(—)1_P12 (SEQ ID NO: 389), as compared to the known protein Dolichyl-diphosphooligosaccharide—protein glycosyltransferase 63 kDa subunit precursor, are described in Table 19 (given according to their position(s) on the amino acid sequence in the first column; the second column indicates whether the glycosylation site is present in the variant protein; and the last column indicates whether the position is different on the variant protein).

TABLE 19 Glycosylation site(s) Position(s) on known amino Present in Position in acid sequence variant protein? variant protein? 106 yes 106

Variant protein T46984_PEA_(—)1_P12 (SEQ ID NO: 389) is encoded by the following transcript(s): T46984_PEA_(—)1_T15 (SEQ ID NO: 319), for which the sequence(s) is/are given at the end of the application. The coding portion of transcript T46984_PEA_(—)1_T15 (SEQ ID NO: 319) is shown in bold; this coding portion starts at position 316 and ends at position 1344. The transcript also has the following SNPs as listed in Table 20 (given according to their position on the nucleotide sequence, with the alternative nucleic acid listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein T46984_PEA_(—)1_P12 (SEQ ID NO: 389) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 20 Nucleic acid SNPs SNP position on nucleotide Alternative Previously sequence nucleic acid known SNP? 28 G -> C No 173 G -> C Yes 256 C -> T Yes 274 G -> C Yes 325 C -> No 389 C -> G Yes 610 G -> A Yes 718 T -> No 724 C -> No 844 C -> T Yes 857 -> G No 885 C -> No 897 -> G No 1002 G -> A No 1048 A -> No 1048 A -> G No 1068 A -> C No 1076 G -> A Yes 1187 A -> No 1187 A -> C No 1220 A -> G No 1220 A -> T No 1254 T -> G No 1291 A -> C No 1293 C -> G No 1303 G -> A No 1505 A -> C No 1535 T -> No 1535 T -> C No 1577 T -> No 1610 A -> C No 1610 A -> T No 1841 C -> No 1841 C -> A No 1848 C -> No 1852 G -> No 2037 C -> A No 2057 T -> C Yes 2151 C -> T No 2184 A -> C No 2196 A -> T No 2211 G -> A Yes 2217 -> A No 2226 A -> G No 2251 T -> G No 2265 T -> A No 2265 T -> G No 2365 -> C No 2374 T -> No 2433 A -> C No

Variant protein T46984_PEA_(—)1_P21 (SEQ ID NO. 390 according to the present invention has an amino acid sequence as given at the end of the application; it is encoded by transcript(s) T46984_PEA_(—)1_T27 (SEQ ID NO: 322). An alignment is given to the known protein (Dolichyl-diphosphooligosaccharide—protein glycosyltransferase 63 kDa subunit precursor) at the end of the application. One or more alignments to one or more previously published protein sequences are given at the end of the application. A brief description of the relationship of the variant protein according to the present invention to each such aligned protein is as follows:

Comparison report between T46984_PEA_(—)1_P21 (SEQ ID NO. 390 and RIB2_HUMAN:

1. An isolated chimeric polypeptide encoding for T46984_PEA_(—)1_P21 (SEQ ID NO. 390, comprising a first amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence M corresponding to amino acids 1-1 of T46984_PEA_(—)1_P21 (SEQ ID NO. 390, and a second amino acid sequence being at least 90% homologous to KACTYIRSNLDPSNVDSLFYAAQASQALSGCEISISNETKDLLLAAVSEDSSVTQIYHAV AALSGFGLPLASQEALSALTARLSKEETVLATVQALQTASHLSQQADLRSIVEEIEDLVA RLDELGGVYLQFEEGLETTALFVAATYKLMDHVGTEPSIKEDQVIQLMNAIFSKKNFES LSEAFSVASAAAVLSHNRYHVPVVVVPEGSASDTHEQAILRLQVTNVLSQPLTQATVKL EHAKSVASRATVLQKTSFTPVGDVFELNFMNVKFSSGYYDFLVEVEGDNRYIANTVEL RVKISTEVGITNVDLSTVDKDQSIAPKTTRVTYPAKAKGTFIADSHQNFALFFQLVDVNT GAELTPHQTFVRLHNQKTGQEVVFVAEPDNKNVYKFELDTSERKIEFDSASGTYTLYLII GDATLKNPILWNVADVVIKFPEEEAPSTVLSQNLFTPKQEIQHLFREPEKRPPTVVSNTF TALILSPLLLLFALWIRIGANVSNFTFAPSTIIFHLGHAAMLGLMYVYWTQLNMFQTLKY LAILGSVTFLAGNRMLAQQAVKRTAH corresponding to amino acids 70-631 of RIB2_HUMAN, which also corresponds to amino acids 2-563 of T46984_PEA_(—)1_P21 (SEQ ID NO. 390, wherein said first amino acid sequence and second amino acid sequence are contiguous and in a sequential order.

The location of the variant protein was determined according to results from a number of different software programs and analyses, including analyses from SignalP and other specialized programs. The variant protein is believed to be located as follows with regard to the cell: membrane. The protein localization is believed to be membrane because both trans-membrane region prediction programs predicted a trans-membrane region for this protein. In addition both signal-peptide prediction programs predict that this protein is a non-secreted protein.

Variant protein T46984_PEA_(—)1_P21 (SEQ ID NO. 390 also has the following non-silent SNPs (Single Nucleotide Polymorphisms) as listed in Table 21, (given according to their position(s) on the amino acid sequence, with the alternative amino acid(s) listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein T46984_PEA_(—)1_P21 (SEQ ID NO. 390 sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 21 Amino acid mutations SNP position(s) on amino acid Alternative Previously sequence amino acid(s) known SNP? 31 G -> R Yes 67 F -> No 69 L -> No 122 R -> No 177 N -> No 177 N -> D No 183 E -> D No 186 S -> N Yes 223 Q -> P No 223 Q -> No 234 Q -> L No 234 Q -> R No 258 T -> P No 262 D -> N No 286 G -> V Yes 357 T -> P No 367 F -> L No 367 F -> No 381 F -> No 392 K -> * No 392 K -> Q No 469 P -> No 469 P -> T No 471 T -> No 472 V -> No 534 T -> N No

The glycosylation sites of variant protein T46984_PEA_(—)1_P21 (SEQ ID NO. 390, as compared to the known protein Dolichyl-diphosphooligosaccharide—protein glycosyltransferase 63 kDa subunit precursor, are described in Table 22 (given according to their position(s) on the amino acid sequence in the first column; the second column indicates whether the glycosylation site is present in the variant protein; and the last column indicates whether the position is different on the variant protein).

TABLE 22 Glycosylation site(s) Position(s) on known amino Present in Position in acid sequence variant protein? variant protein? 106 yes 38

Variant protein T46984_PEA_(—)1_P21 (SEQ ID NO. 390 is encoded by the following transcript(s): T46984_PEA_(—)1_T27 (SEQ ID NO: 322), for which the sequence(s) is/are given at the end of the application. The coding portion of transcript T46984_PEA_(—)1_T27 (SEQ ID NO: 322) is shown in bold; this coding portion starts at position 338 and ends at position 2026. The transcript also has the following SNPs as listed in Table 23 (given according to their position on the nucleotide sequence, with the alternative nucleic acid listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein T46984_PEA_(—)1_P21 (SEQ ID NO. 390 sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 23 Nucleic acid SNPs SNP position on nucleotide Alternative Previously sequence nucleic acid known SNP? 68 C -> T Yes 194 A -> G Yes 428 G -> A Yes 536 T -> No 542 C -> No 662 C -> T Yes 675 -> G No 703 C -> No 715 -> G No 820 G -> A No 866 A -> No 866 A -> G No 886 A -> C No 894 G -> A Yes 1005 A -> No 1005 A -> C No 1038 A -> G No 1038 A -> T No 1072 T -> G No 1109 A -> C No 1111 C -> G No 1121 G -> A No 1194 G -> T Yes 1406 A -> C No 1436 T -> No 1436 T -> C No 1478 T -> No 1511 A -> C No 1511 A -> T No 1742 C -> No 1742 C -> A No 1749 C -> No 1753 G -> No 1938 C -> A No 1958 T -> C Yes 2052 C -> T No 2085 A -> C No 2097 A -> T No 2112 G -> A Yes 2118 -> A No 2127 A -> G No 2152 T -> G No 2166 T -> A No 2166 T -> G No 2266 -> C No 2275 T -> No 2334 A -> C No

Variant protein T46984_PEA_(—)1_P27 (SEQ ID NO: 391) according to the present invention has an amino acid sequence as given at the end of the application; it is encoded by transcript(s) T46984_PEA_(—)1_T34 (SEQ ID NO: 324). An alignment is given to the known protein (Dolichyl-diphosphooligosaccharide—protein glycosyltransferase 63 kDa subunit precursor) at the end of the application. One or more alignments to one or more previously published protein sequences are given at the end of the application. A brief description of the relationship of the variant protein according to the present invention to each such aligned protein is as follows:

Comparison report between T46984_PEA_(—)1_P27 (SEQ ID NO: 391) and RIB2_HUMAN:

1. An isolated chimeric polypeptide encoding for T46984_PEA_(—)1_P27 (SEQ ID NO: 391), comprising a first amino acid sequence being at least 90% homologous to MAPPGSSTVFLLALTIIASTWALTPTHYLTKHDVERLKASLDRPFTNLESAFYSIVGLSSL GAQVPDAKKACTYIRSNLDPSNVDSLFYAAQASQALSGCEISISNETKDLLLAAVSEDSS VTQIYHAVAALSGFGLPLASQEALSALTARLSKEETVLATVQALQTASHLSQQADLRSI VEEIEDLVARLDELGGVYLQFEEGLETTALFVAATYKLMDHVGTEPSIKEDQVIQLMNA IFSKKNFESLSEAFSVASAAAVLSHNRYHVPVVVVPEGSASDTHEQAILRLQVTNVLSQ PLTQATVKLEHAKSVASRATVLQKTSFTPVGDVFELNFMNVKFSSGYYDFLVEVEGDN RYIANTVELRVKISTEVGITNVDLSTVDKDQSIAPKTTRVTYPAKAKGTFIADSHQNFA corresponding to amino acids 1-415 of RIB2_HUMAN, which also corresponds to amino acids 1-415 of T46984_PEA_(—)1_P27 (SEQ ID NO: 391), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence FGSGLVPMSPTSLLLLARLYFTWDMLLCWDSCMSTGLSSTCSRP (SEQ ID NO: 1064) corresponding to amino acids 416-459 of T46984_PEA_(—)1_P27 (SEQ ID NO: 391), wherein said first amino acid sequence and second amino acid sequence are contiguous and in a sequential order.

2. An isolated polypeptide encoding for a tail of T46984_PEA_(—)1_P27 (SEQ ID NO: 391), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence FGSGLVPMSPTSLLLLARLYFTWDMLLCWDSCMSTGLSSTCSRP (SEQ ID NO: 1064) in T46984_PEA_(—)1_P27 (SEQ ID NO: 391).

The location of the variant protein was determined according to results from a number of different software programs and analyses, including analyses from SignalP and other specialized programs. The variant protein is believed to be located as follows with regard to the cell: secreted. The protein localization is believed to be secreted because both signal-peptide prediction programs predict that this protein has a signal peptide, and neither trans-membrane region prediction program predicts that this protein has a trans-membrane region.

Variant protein T46984_PEA_(—)1_P27 (SEQ ID NO: 391) also has the following non-silent SNPs (Single Nucleotide Polymorphisms) as listed in Table 24, (given according to their position(s) on the amino acid sequence, with the alternative amino acid(s) listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein T46984_PEA_(—)1_P27 (SEQ ID NO: 391) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 24 Amino acid mutations SNP position(s) on amino acid Alternative Previously sequence amino acid(s) known SNP? 4 P -> No 25 P -> R Yes 99 G -> R Yes 135 F -> No 137 L -> No 190 R -> No 245 N -> No 245 N -> D No 251 E -> D No 254 S -> N Yes 291 Q -> No 291 Q -> P No 302 Q -> R No 302 Q -> L No 326 T -> P No 330 D -> N No 354 G -> V Yes 459 P -> T No

The glycosylation sites of variant protein T46984_PEA_(—)1_P27 (SEQ ID NO: 391), as compared to the known protein Dolichyl-diphosphooligosaccharide—protein glycosyltransferase 63 kDa subunit precursor, are described in Table 25 (given according to their position(s) on the amino acid sequence in the first column; the second column indicates whether the glycosylation site is present in the variant protein; and the last column indicates whether the position is different on the variant protein).

TABLE 25 Glycosylation site(s) Position(s) on known amino Present in Position in acid sequence variant protein? variant protein? 106 yes 106

Variant protein T46984_PEA_(—)1_P27 (SEQ ID NO: 391) is encoded by the following transcript(s): T46984_PEA_(—)1_T34 (SEQ ID NO: 324), for which the sequence(s) is/are given at the end of the application. The coding portion of transcript T46984_PEA_(—)1_T34 (SEQ ID NO: 324) is shown in bold; this coding portion starts at position 316 and ends at position 1692. The transcript also has the following SNPs as listed in Table 26 (given according to their position on the nucleotide sequence, with the alternative nucleic acid listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein T46984_PEA_(—)1_P27 (SEQ ID NO: 391) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 26 Nucleic acid SNPs SNP position on nucleotide Alternative Previously sequence nucleic acid known SNP? 28 G -> C No 173 G -> C Yes 256 C -> T Yes 274 G -> C Yes 325 C -> No 389 C -> G Yes 610 G -> A Yes 718 T -> No 724 C -> No 844 C -> T Yes 857 -> G No 885 C -> No 897 -> G No 1002 G -> A No 1048 A -> No 1048 A -> G No 1068 A -> C No 1076 G -> A Yes 1187 A -> No 1187 A -> C No 1220 A -> G No 1220 A -> T No 1254 T -> G No 1291 A -> C No 1293 C -> G No 1303 G -> A No 1376 G -> T Yes 1690 C -> A No 1710 T -> C Yes 1804 C -> T No 1837 A -> C No 1849 A -> T No 1864 G -> A Yes 1870 -> A No 1879 A -> G No 1904 T -> G No 1918 T -> A No 1918 T -> G No 2018 -> C No 2027 T -> No 2086 A -> C No

Variant protein T46984_PEA_(—)1_P32 (SEQ ID NO: 392) according to the present invention has an amino acid sequence as given at the end of the application; it is encoded by transcript(s) T46984_PEA_(—)1_T40 (SEQ ID NO: 326). An alignment is given to the known protein (Dolichyl-diphosphooligosaccharide—protein glycosyltransferase 63 kDa subunit precursor) at the end of the application. One or more alignments to one or more previously published protein sequences are given at the end of the application. A brief description of the relationship of the variant protein according to the present invention to each such aligned protein is as follows:

Comparison report between T46984_PEA_(—)1_P32 (SEQ ID NO: 392) and RIB2_HUMAN:

1. An isolated chimeric polypeptide encoding for T46984_PEA_(—)1_P32 (SEQ ID NO: 392), comprising a first amino acid sequence being at least 90% homologous to MAPPGSSTVFLLALTIIASTWALTPTHYLTKHDVERLKASLDRPFTNLESAFYSIVGLSSL GAQVPDAKKACTYIRSNLDPSNVDSLFYAAQASQALSGCEISISNETKDLLLAAVSEDSS VTQIYHAVAALSGFGLPLASQEALSALTARLSKEETVLATVQALQTASHLSQQADLRSI VEEIEDLVARLDELGGVYLQFEEGLETTALFVAATYKLMDHVGTEPSIKEDQVIQLMNA IFSKKNFESLSEAFSVASAAAVLSHNRYHVPVVVVPEGSASDTHEQAILRLQVTNVLSQ PLTQATVKLEHAKSVASRATVLQKTSFTPVGDVFELNFMNVKFSSGYYDFLVEVEGDN RYIANTVE corresponding to amino acids 1-364 of RIB2_HUMAN, which also corresponds to amino acids 1-364 of T46984_PEA_(—)1_P32 (SEQ ID NO: 392), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence GQVRWLTPVIPALWEAKAGGSPEVRSSILAWPT (SEQ ID NO: 1065) corresponding to amino acids 365-397 of T46984_PEA_(—)1_P32 (SEQ ID NO: 392), wherein said first amino acid sequence and second amino acid sequence are contiguous and in a sequential order.

2. An isolated polypeptide encoding for a tail of T46984_PEA_(—)1_P32 (SEQ ID NO: 392), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence GQVRWLTPVIPALWEAKAGGSPEVRSSILAWPT (SEQ ID NO: 1065) in T46984_PEA_(—)1_P32 (SEQ ID NO: 392).

The location of the variant protein was determined according to results from a number of different software programs and analyses, including analyses from SignalP and other specialized programs. The variant protein is believed to be located as follows with regard to the cell: secreted. The protein localization is believed to be secreted because both signal-peptide prediction programs predict that this protein has a signal peptide, and neither trans-membrane region prediction program predicts that this protein has a trans-membrane region.

Variant protein T46984_PEA_(—)1_P32 (SEQ ID NO: 392) also has the following non-silent SNPs (Single Nucleotide Polymorphisms) as listed in Table 27, (given according to their position(s) on the amino acid sequence, with the alternative amino acid(s) listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein T46984_PEA_(—)1_P32 (SEQ ID NO: 392) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 27 Amino acid mutations SNP position(s) on amino acid Alternative Previously sequence amino acid(s) known SNP? 4 P -> No 25 P -> R Yes 99 G -> R Yes 135 F -> No 137 L -> No 190 R -> No 245 N -> No 245 N -> D No 251 E -> D No 254 S -> N Yes 291 Q -> No 291 Q -> P No 302 Q -> R No 302 Q -> L No 326 T -> P No 330 D -> N No 354 G -> V Yes

The glycosylation sites of variant protein T46984_PEA_(—)1_P32 (SEQ ID NO: 392), as compared to the known protein Dolichyl-diphosphooligosaccharide—protein glycosyltransferase 63 kDa subunit precursor, are described in Table 28 (given according to their position(s) on the amino acid sequence in the first column; the second column indicates whether the glycosylation site is present in the variant protein; and the last column indicates whether the position is different on the variant protein).

TABLE 28 Glycosylation site(s) Position(s) on known amino Present in Position in acid sequence variant protein? variant protein? 106 yes 106

Variant protein T46984_PEA_(—)1_P32 (SEQ ID NO: 392) is encoded by the following transcript(s): T46984_PEA_(—)1_T40 (SEQ ID NO: 326), for which the sequence(s) is/are given at the end of the application. The coding portion of transcript T46984_PEA_(—)1_T40 (SEQ ID NO: 326) is shown in bold; this coding portion starts at position 316 and ends at position 1506. The transcript also has the following SNPs as listed in Table 29 (given according to their position on the nucleotide sequence, with the alternative nucleic acid listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein T46984_PEA_(—)1_P32 (SEQ ID NO: 392) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 29 Nucleic acid SNPs SNP position on nucleotide Alternative Previously sequence nucleic acid known SNP? 28 G -> C No 173 G -> C Yes 256 C -> T Yes 274 G -> C Yes 325 C -> No 389 C -> G Yes 610 G -> A Yes 718 T -> No 724 C -> No 844 C -> T Yes 857 -> G No 885 C -> No 897 -> G No 1002 G -> A No 1048 A -> No 1048 A -> G No 1068 A -> C No 1076 G -> A Yes 1187 A -> No 1187 A -> C No 1220 A -> G No 1220 A -> T No 1254 T -> G No 1291 A -> C No 1293 C -> G No 1303 G -> A No 1376 G -> T Yes

Variant protein T46984_PEA_(—)1_P34 (SEQ ID NO: 393) according to the present invention has an amino acid sequence as given at the end of the application; it is encoded by transcript(s) T46984_PEA_(—)1_T42 (SEQ ID NO: 327). An alignment is given to the known protein (Dolichyl-diphosphooligosaccharide—protein glycosyltransferase 63 kDa subunit precursor) at the end of the application. One or more alignments to one or more previously published protein sequences are given at the end of the application. A brief description of the relationship of the variant protein according to the present invention to each such aligned protein is as follows:

Comparison report between T46984_PEA_(—)1_P34 (SEQ ID NO: 393) and RIB2_HUMAN:

1. An isolated chimeric polypeptide encoding for T46984_PEA_(—)1_P34 (SEQ ID NO: 393), comprising a first amino acid sequence being at least 90% homologous to MAPPGSSTVFLLALTIIASTWALTPTHYLTKHDVERLKASLDRPFTNLESAFYSIVGLSSL GAQVPDAKKACTYIRSNLDPSNVDSLFYAAQASQALSGCEISISNETKDLLLAAVSEDSS VTQIYHAVAALSGFGLPLASQEALSALTARLSKEETVLATVQALQTASHLSQQADLRSI VEEIEDLVARLDELGGVYLQFEEGLETTALFVAATYKLMDHVGTEPSIKEDQVIQLMNA IFSKKNFESLSEAFSVASAAAVLSHNRYHVPVVVVPEGSASDTHEQAILRLQVTNVLSQ PLTQATVKLEHAKSVASRATVLQKTSFTPVG corresponding to amino acids 1-329 of RIB2_HUMAN, which also corresponds to amino acids 1-329 of T46984_PEA_(—)1_P34 (SEQ ID NO: 393).

The location of the variant protein was determined according to results from a number of different software programs and analyses, including analyses from SignalP and other specialized programs. The variant protein is believed to be located as follows with regard to the cell: secreted. The protein localization is believed to be secreted because both signal-peptide prediction programs predict that this protein has a signal peptide, and neither trans-membrane region prediction program predicts that this protein has a trans-membrane region.

Variant protein T46984_PEA_(—)1_P34 (SEQ ID NO: 393) also has the following non-silent SNPs (Single Nucleotide Polymorphisms) as listed in Table 30, (given according to their position(s) on the amino acid sequence, with the alternative amino acid(s) listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein T46984_PEA_(—)1_P34 (SEQ ID NO: 393) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 30 Amino acid mutations SNP position(s) on amino acid Alternative Previously sequence amino acid(s) known SNP? 4 P -> No 25 P -> R Yes 99 G -> R Yes 135 F -> No 137 L -> No 190 R -> No 245 N -> No 245 N -> D No 251 E -> D No 254 S -> N Yes 291 Q -> No 291 Q -> P No 302 Q -> L No 302 Q -> R No 326 T -> P No

The glycosylation sites of variant protein T46984_PEA_(—)1_P34 (SEQ ID NO: 393), as compared to the known protein Dolichyl-diphosphooligosaccharide—protein glycosyltransferase 63 kDa subunit precursor, are described in Table 31 (given according to their position(s) on the amino acid sequence in the first column; the second column indicates whether the glycosylation site is present in the variant protein; and the last column indicates whether the position is different on the variant protein).

TABLE 31 Glycosylation site(s) Position(s) on known amino Present in Position in acid sequence variant protein? variant protein? 106 yes 106

Variant protein T46984_PEA_(—)1_P34 (SEQ ID NO: 393) is encoded by the following transcript(s): T46984_PEA_(—)1_T42 (SEQ ID NO: 327), for which the sequence(s) is/are given at the end of the application. The coding portion of transcript T46984_PEA_(—)1_T42 (SEQ ID NO: 327) is shown in bold; this coding portion starts at position 316 and ends at position 1302. The transcript also has the following SNPs as listed in Table 32 (given according to their position on the nucleotide sequence, with the alternative nucleic acid listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein T46984_PEA_(—)1_P34 (SEQ ID NO: 393) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 32 Nucleic acid SNPs SNP position on nucleotide Alternative Previously sequence nucleic acid known SNP? 28 G -> C No 173 G -> C Yes 256 C -> T Yes 274 G -> C Yes 325 C -> No 389 C -> G Yes 610 G -> A Yes 718 T -> No 724 C -> No 844 C -> T Yes 857 -> G No 885 C -> No 897 -> G No 1002 G -> A No 1048 A -> No 1048 A -> G No 1068 A -> C No 1076 G -> A Yes 1187 A -> No 1187 A -> C No 1220 A -> G No 1220 A -> T No 1254 T -> G No 1291 A -> C No 1293 C -> G No 1324 T -> C Yes 1489 G -> A Yes

Variant protein T46984_PEA_(—)1_P35 (SEQ ID NO: 394) according to the present invention has an amino acid sequence as given at the end of the application; it is encoded by transcript(s) T46984_PEA_(—)1_T43 (SEQ ID NO: 328). An alignment is given to the known protein (Dolichyl-diphosphooligosaccharide—protein glycosyltransferase 63 kDa subunit precursor) at the end of the application. One or more alignments to one or more previously published protein sequences are given at the end of the application. A brief description of the relationship of the variant protein according to the present invention to each such aligned protein is as follows:

Comparison report between T46984_PEA_(—)1_P35 (SEQ ID NO: 394) and RIB2_HUMAN:

1. An isolated chimeric polypeptide encoding for T46984_PEA_(—)1_P35 (SEQ ID NO: 394), comprising a first amino acid sequence being at least 90% homologous to MAPPGSSTVFLLALTIIASTWALTPTHYLTKHDVERLKASLDRPFTNLESAFYSIVGLSSL GAQVPDAKKACTYIRSNLDPSNVDSLFYAAQASQALSGCEISISNETKDLLLAAVSEDSS VTQIYHAVAALSGFGLPLASQEALSALTARLSKEETVLATVQALQTASHLSQQADLRSI VEEIEDLVARLDELGGVYLQFEEGLETTALFVAATYKLMDHVGTEPSIKEDQVIQLMNA IFSKKNFESLSEAFSVASAAAVLSHNRYHVPVVVVPEGSASDTHEQAI corresponding to amino acids 1-287 of RIB2_HUMAN, which also corresponds to amino acids 1-287 of T46984_PEA_(—)1_P35 (SEQ ID NO: 394), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence GCWPSRQSREQHISSRRKMEILKTECQEKESRTIHSMRRKMEKKNFI (SEQ ID NO: 1066) corresponding to amino acids 288-334 of T46984_PEA_(—)1_P35 (SEQ ID NO: 394), wherein said first amino acid sequence and second amino acid sequence are contiguous and in a sequential order.

2. An isolated polypeptide encoding for a tail of T46984_PEA_(—)1_P35 (SEQ ID NO: 394), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence GCWPSRQSREQHISSRRKMEILKTECQEKESRTIHSMRRKMEKKNFI (SEQ ID NO: 1066) in T46984_PEA_(—)1_P35 (SEQ ID NO: 394).

The location of the variant protein was determined according to results from a number of different software programs and analyses, including analyses from SignalP and other specialized programs. The variant protein is believed to be located as follows with regard to the cell: secreted. The protein localization is believed to be secreted because both signal-peptide prediction programs predict that this protein has a signal peptide, and neither trans-membrane region prediction program predicts that this protein has a trans-membrane region.

Variant protein T46984_PEA_(—)1_P35 (SEQ ID NO: 394) also has the following non-silent SNPs (Single Nucleotide Polymorphisms) as listed in Table 33, (given according to their position(s) on the amino acid sequence, with the alternative amino acid(s) listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein T46984_PEA_(—)1_P35 (SEQ ID NO: 394) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 33 Amino acid mutations SNP position(s) on amino acid Previously sequence Alternative amino acid(s) known SNP? 4 P -> No 25 P -> R Yes 99 G -> R Yes 135 F -> No 137 L -> No 190 R -> No 245 N -> No 245 N -> D No 251 E -> D No 254 S -> N Yes 320 T -> P No 324 M -> L No 329 E -> K Yes 334 I -> V No

The glycosylation sites of variant protein T46984_PEA_(—)1_P35 (SEQ ID NO: 394), as compared to the known protein Dolichyl-diphosphooligosaccharide—protein glycosyltransferase 63 kDa subunit precursor, are described in Table 34 (given according to their position(s) on the amino acid sequence in the first column; the second column indicates whether the glycosylation site is present in the variant protein; and the last column indicates whether the position is different on the variant protein).

TABLE 34 Glycosylation site(s) Position(s) on known amino Present in acid sequence variant protein? Position in variant protein? 106 yes 106

Variant protein T46984_PEA_(—)1_P35 (SEQ ID NO: 394) is encoded by the following transcript(s): T46984_PEA_(—)1_T43 (SEQ ID NO: 328), for which the sequence(s) is/are given at the end of the application. The coding portion of transcript T46984_PEA_(—)1_T43 (SEQ ID NO: 328) is shown in bold; this coding portion starts at position 316 and ends at position 1317. The transcript also has the following SNPs as listed in Table 35 (given according to their position on the nucleotide sequence, with the alternative nucleic acid listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein T46984_PEA_(—)1_P35 (SEQ ID NO: 394) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 35 Nucleic acid SNPs SNP position on nucleotide Alternative sequence nucleic acid Previously known SNP? 28 G -> C No 173 G -> C Yes 256 C -> T Yes 274 G -> C Yes 325 C -> No 389 C -> G Yes 610 G -> A Yes 718 T -> No 724 C -> No 844 C -> T Yes 857 -> G No 885 C -> No 897 -> G No 1002 G -> A No 1048 A -> No 1048 A -> G No 1068 A -> C No 1076 G -> A Yes 1240 C -> T No 1273 A -> C No 1285 A -> T No 1300 G -> A Yes 1306 -> A No 1315 A -> G No 1340 T -> G No 1354 T -> A No 1354 T -> G No 1454 -> C No 1463 T -> No 1522 A -> C No

Variant protein T46984_PEA_(—)1_P38 (SEQ ID NO: 395) according to the present invention has an amino acid sequence as given at the end of the application; it is encoded by transcript(s) T46984_PEA_(—)1_T47 (SEQ ID NO: 330). An alignment is given to the known protein (Dolichyl-diphosphooligosaccharide—protein glycosyltransferase 63 kDa subunit precursor) at the end of the application. One or more alignments to one or more previously published protein sequences are given at the end of the application. A brief description of the relationship of the variant protein according to the present invention to each such aligned protein is as follows:

Comparison report between T46984_PEA_(—)1_P38 (SEQ ID NO: 395) and RIB2_HUMAN:

1. An isolated chimeric polypeptide encoding for T46984_PEA_(—)1_P38 (SEQ ID NO: 395), comprising a first amino acid sequence being at least 90% homologous to MAPPGSSTVFLLALTIIASTWALTPTHYLTKHDVERLKASLDRPFTNLESAFYSIVGLSSL GAQVPDAKKACTYIRSNLDPSNVDSLFYAAQASQALSGCEISISNETKDLLLAAVSEDSS VTQIYHAVAALSGFGLPLASQEAL corresponding to amino acids 1-145 of RIB2_HUMAN, which also corresponds to amino acids 1-145 of T46984_PEA_(—)1_P38 (SEQ ID NO: 395), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence MDPDWCQCLQLHFCS (SEQ ID NO: 1067) corresponding to amino acids 146-160 of T46984_PEA_(—)1_P38 (SEQ ID NO: 395), wherein said first amino acid sequence and second amino acid sequence are contiguous and in a sequential order.

2. An isolated polypeptide encoding for a tail of T46984_PEA_(—)1_P38 (SEQ ID NO: 395), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence MDPDWCQCLQLHFCS (SEQ ID NO: 1067) in T46984_PEA_(—)1_P38 (SEQ ID NO: 395).

The location of the variant protein was determined according to results from a number of different software programs and analyses, including analyses from SignalP and other specialized programs. The variant protein is believed to be located as follows with regard to the cell: secreted. The protein localization is believed to be secreted because both signal-peptide prediction programs predict that this protein has a signal peptide, and neither trans-membrane region prediction program predicts that this protein has a trans-membrane region.

Variant protein T46984_PEA_(—)1_P38 (SEQ ID NO: 395) also has the following non-silent SNPs (Single Nucleotide Polymorphisms) as listed in Table 36, (given according to their position(s) on the amino acid sequence, with the alternative amino acid(s) listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein T46984_PEA_(—)1_P38 (SEQ ID NO: 395) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 36 Amino acid mutations SNP position(s) on amino acid Alternative sequence amino acid(s) Previously known SNP? 4 P -> No 25 P -> R Yes 99 G -> R Yes 135 F -> No 137 L -> No

The glycosylation sites of variant protein T46984_PEA_(—)1_P38 (SEQ ID NO: 395), as compared to the known protein Dolichyl-diphosphooligosaccharide—protein glycosyltransferase 63 kDa subunit precursor, are described in Table 37 (given according to their position(s) on the amino acid sequence in the first column; the second column indicates whether the glycosylation site is present in the variant protein; and the last column indicates whether the position is different on the variant protein).

TABLE 37 Glycosylation site(s) Position(s) on known amino Present in acid sequence variant protein? Position in variant protein? 106 yes 106

Variant protein T46984_PEA_(—)1_P38 (SEQ ID NO: 395) is encoded by the following transcript(s): T46984_PEA_(—)1_T47 (SEQ ID NO: 330), for which the sequence(s) is/are given at the end of the application. The coding portion of transcript T46984_PEA_(—)1_T47 (SEQ ID NO: 330) is shown in bold; this coding portion starts at position 316 and ends at position 795. The transcript also has the following SNPs as listed in Table 38 (given according to their position on the nucleotide sequence, with the alternative nucleic acid listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein T46984_PEA_(—)1_P38 (SEQ ID NO: 395) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 38 Nucleic acid SNPs SNP position on nucleotide Alternative sequence nucleic acid Previously known SNP? 28 G -> C No 173 G -> C Yes 256 C -> T Yes 274 G -> C Yes 325 C -> No 389 C -> G Yes 610 G -> A Yes 718 T -> No 724 C -> No 879 C -> A No 899 T -> C Yes 993 C -> T No 1026 A -> C No 1038 A -> T No 1053 G -> A Yes 1059 -> A No 1068 A -> G No 1093 T -> G No 1107 T -> A No 1107 T -> G No 1207 -> C No 1216 T -> No 1275 A -> C No

Variant protein T46984_PEA_(—)1_P39 (SEQ ID NO: 396) according to the present invention has an amino acid sequence as given at the end of the application; it is encoded by transcript(s) T46984_PEA_(—)1_T48 (SEQ ID NO: 331). An alignment is given to the known protein (Dolichyl-diphosphooligosaccharide—protein glycosyltransferase 63 kDa subunit precursor) at the end of the application. One or more alignments to one or more previously published protein sequences are given at the end of the application. A brief description of the relationship of the variant protein according to the present invention to each such aligned protein is as follows:

Comparison report between T46984_PEA_(—)1_P39 (SEQ ID NO: 396) and RIB2_HUMAN:

1. An isolated chimeric polypeptide encoding for T46984_PEA_(—)1_P39 (SEQ ID NO: 396), comprising a first amino acid sequence being at least 90% homologous to MAPPGSSTVFLLALTIIASTWALTPTHYLTKHDVERLKASLDRPFTNLESAFYSIVGLSSL GAQVPDAKKACTYIRSNLDPSNVDSLFYAAQASQALSGCEISISNETKDLLLAAVSEDSS VTQIYHAVAALSGFGLPLASQEALSALTARLSKEETVLA corresponding to amino acids 1-160 of RIB2_HUMAN, which also corresponds to amino acids 1-160 of T46984_PEA_(—)1_P39 (SEQ ID NO: 396).

The location of the variant protein was determined according to results from a number of different software programs and analyses, including analyses from SignalP and other specialized programs. The variant protein is believed to be located as follows with regard to the cell: secreted. The protein localization is believed to be secreted because both signal-peptide prediction programs predict that this protein has a signal peptide, and neither trans-membrane region prediction program predicts that this protein has a trans-membrane region.

Variant protein T46984_PEA_(—)1_P39 (SEQ ID NO: 396) also has the following non-silent SNPs (Single Nucleotide Polymorphisms) as listed in Table 39, (given according to their position(s) on the amino acid sequence, with the alternative amino acid(s) listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein T46984_PEA_(—)1_P39 (SEQ ID NO: 396) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 39 Amino acid mutations SNP position(s) on amino acid Alternative sequence amino acid(s) Previously known SNP? 4 P -> No 25 P -> R Yes 99 G -> R Yes 135 F -> No 137 L -> No

The glycosylation sites of variant protein T46984_PEA_(—)1_P39 (SEQ ID NO: 396), as compared to the known protein Dolichyl-diphosphooligosaccharide—protein glycosyltransferase 63 kDa subunit precursor, are described in Table 40 (given according to their position(s) on the amino acid sequence in the first column; the second column indicates whether the glycosylation site is present in the variant protein; and the last column indicates whether the position is different on the variant protein).

TABLE 40 Glycosylation site(s) Position(s) on known amino Present in acid sequence variant protein? Position in variant protein? 106 yes 106

Variant protein T46984_PEA_(—)1_P39 (SEQ ID NO: 396) is encoded by the following transcript(s): T46984_PEA_(—)1_T48 (SEQ ID NO: 331), for which the sequence(s) is/are given at the end of the application. The coding portion of transcript T46984_PEA_(—)1_T48 (SEQ ID NO: 331) is shown in bold; this coding portion starts at position 316 and ends at position 795. The transcript also has the following SNPs as listed in Table 41 (given according to their position on the nucleotide sequence, with the alternative nucleic acid listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein T46984_PEA_(—)1_P39 (SEQ ID NO: 396) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 41 Nucleic acid SNPs SNP position on nucleotide Previously sequence Alternative nucleic acid known SNP? 28 G -> C No 173 G -> C Yes 256 C -> T Yes 274 G -> C Yes 325 C -> No 389 C -> G Yes 610 G -> A Yes 718 T -> No 724 C -> No 848 G -> T Yes 879 C -> G Yes 1008 A -> G Yes 1397 A -> G Yes

Variant protein T46984_PEA_(—)1_P45 (SEQ ID NO: 397) according to the present invention has an amino acid sequence as given at the end of the application; it is encoded by transcript(s) T46984_PEA_(—)1_T32 (SEQ ID NO: 323). An alignment is given to the known protein (Dolichyl-diphosphooligosaccharide—protein glycosyltransferase 63 kDa subunit precursor) at the end of the application. One or more alignments to one or more previously published protein sequences are given at the end of the application. A brief description of the relationship of the variant protein according to the present invention to each such aligned protein is as follows:

Comparison report between T46984_PEA_(—)1_P45 (SEQ ID NO: 397) and RIB2_HUMAN:

1. An isolated chimeric polypeptide encoding for T46984_PEA_(—)1_P45 (SEQ ID NO: 397), comprising a first amino acid sequence being at least 90% homologous to MAPPGSSTVFLLALTIIASTWALTPTHYLTKHDVERLKASLDRPFTNLESAFYSIVGLSSL GAQVPDAKKACTYIRSNLDPSNVDSLFYAAQASQALSGCE corresponding to amino acids 1-101 of RIB2_HUMAN, which also corresponds to amino acids 1-101 of T46984_PEA_(—)1_P45 (SEQ ID NO: 397), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence NSPGSADSIPPVPAG (SEQ ID NO: 1068) corresponding to amino acids 102-116 of T46984_PEA_(—)1_P45 (SEQ ID NO: 397), wherein said first amino acid sequence and second amino acid sequence are contiguous and in a sequential order.

2. An isolated polypeptide encoding for a tail of T46984_PEA_(—)1_P45 (SEQ ID NO: 397), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence NSPGSADSIPPVPAG (SEQ ID NO: 1068) in T46984_PEA_(—)1_P45 (SEQ ID NO: 397).

The location of the variant protein was determined according to results from a number of different software programs and analyses, including analyses from SignalP and other specialized programs. The variant protein is believed to be located as follows with regard to the cell: secreted. The protein localization is believed to be secreted because both signal-peptide prediction programs predict that this protein has a signal peptide, and neither trans-membrane region prediction program predicts that this protein has a trans-membrane region.

Variant protein T46984_PEA_(—)1_P45 (SEQ ID NO: 397) also has the following non-silent SNPs (Single Nucleotide Polymorphisms) as listed in Table 42, (given according to their position(s) on the amino acid sequence, with the alternative amino acid(s) listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein T46984_PEA_(—)1_P45 (SEQ ID NO: 397) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 42 Amino acid mutations SNP position(s) on amino acid Previously sequence Alternative amino acid(s) known SNP? 4 P -> No 25 P -> R Yes 99 G -> R Yes

The glycosylation sites of variant protein T46984_PEA_(—)1_P45 (SEQ ID NO: 397), as compared to the known protein Dolichyl-diphosphooligosaccharide—protein glycosyltransferase 63 kDa subunit precursor, are described in Table 43 (given according to their position(s) on the amino acid sequence in the first column; the second column indicates whether the glycosylation site is present in the variant protein; and the last column indicates whether the position is different on the variant protein).

TABLE 43 Glycosylation site(s) Position(s) on known amino acid sequence Present in variant protein? 106 no

Variant protein T46984_PEA_(—)1_P45 (SEQ ID NO: 397) is encoded by the following transcript(s): T46984_PEA_(—)1_T32 (SEQ ID NO: 323), for which the sequence(s) is/are given at the end of the application. The coding portion of transcript T46984_PEA_(—)1_T32 (SEQ ID NO: 323) is shown in bold; this coding portion starts at position 316 and ends at position 663. The transcript also has the following SNPs as listed in Table 44 (given according to their position on the nucleotide sequence, with the alternative nucleic acid listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein T46984_PEA_(—)1_P45 (SEQ ID NO: 397) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 44 Nucleic acid SNPs SNP position on nucleotide Previously known sequence Alternative nucleic acid SNP? 28 G -> C No 173 G -> C Yes 256 C -> T Yes 274 G -> C Yes 325 C -> No 389 C -> G Yes 610 G -> A Yes 668 C -> T Yes 681 -> G No 709 C -> No 721 -> G No 826 G -> A No 872 A -> No 872 A -> G No 892 A -> C No 900 G -> A Yes 1011 A -> No 1011 A -> C No 1044 A -> G No 1044 A -> T No 1078 T -> G No 1115 A -> C No 1117 C -> G No 1127 G -> A No 1200 G -> T Yes 1412 A -> C No 1442 T -> No 1442 T -> C No 1484 T -> No 1517 A -> C No 1517 A -> T No 1748 C -> No 1748 C -> A No 1755 C -> No 1759 G -> No 1944 C -> A No 1964 T -> C Yes 2058 C -> T No 2091 A -> C No 2103 A -> T No 2118 G -> A Yes 2124 -> A No 2133 A -> G No 2158 T -> G No 2172 T -> A No 2172 T -> G No 2272 -> C No 2281 T -> No 2340 A -> C No

Variant protein T46984_PEA_(—)1_P46 (SEQ ID NO: 398) according to the present invention has an amino acid sequence as given at the end of the application; it is encoded by transcript(s) T46984_PEA_(—)1_T35 (SEQ ID NO: 325). An alignment is given to the known protein (Dolichyl-diphosphooligosaccharide—protein glycosyltransferase 63 kDa subunit precursor) at the end of the application. One or more alignments to one or more previously published protein sequences are given at the end of the application. A brief description of the relationship of the variant protein according to the present invention to each such aligned protein is as follows:

Comparison report between T46984_PEA_(—)1_P46 (SEQ ID NO: 398) and RIB2_HUMAN:

1. An isolated chimeric polypeptide encoding for T46984_PEA_(—)1_P46 (SEQ ID NO: 398), comprising a first amino acid sequence being at least 90% homologous to MAPPGSSTVFLLALTIIASTWALTPTHYLTKHDVERLKASLDRPFTNLESAFYSIVGLSSL GAQVPDAK corresponding to amino acids 1-69 of RIB2_HUMAN, which also corresponds to amino acids 1-69 of T46984_PEA_(—)1_P46 (SEQ ID NO: 398), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence NSPGSADSIPPVPAG (SEQ ID NO: 1068) corresponding to amino acids 70-84 of T46984_PEA_(—)1_P46 (SEQ ID NO: 398), wherein said first amino acid sequence and second amino acid sequence are contiguous and in a sequential order.

2. An isolated polypeptide encoding for a tail of T46984_PEA_(—)1_P46 (SEQ ID NO: 398), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence NSPGSADSIPPVPAG (SEQ ID NO: 1068) in T46984_PEA_(—)1_P46 (SEQ ID NO: 398).

The location of the variant protein was determined according to results from a number of different software programs and analyses, including analyses from SignalP and other specialized programs. The variant protein is believed to be located as follows with regard to the cell: secreted. The protein localization is believed to be secreted because both signal-peptide prediction programs predict that this protein has a signal peptide, and neither trans-membrane region prediction program predicts that this protein has a trans-membrane region.

Variant protein T46984_PEA_(—)1_P46 (SEQ ID NO: 398) also has the following non-silent SNPs (Single Nucleotide Polymorphisms) as listed in Table 45, (given according to their position(s) on the amino acid sequence, with the alternative amino acid(s) listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein T46984_PEA_(—)1_P46 (SEQ ID NO: 398) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 45 Amino acid mutations SNP position(s) on amino acid Alternative Previously known sequence amino acid(s) SNP? 4 P -> No 25 P -> R Yes

The glycosylation sites of variant protein T46984_PEA_(—)1_P46 (SEQ ID NO: 398), as compared to the known protein Dolichyl-diphosphooligosaccharide—protein glycosyltransferase 63 kDa subunit precursor, are described in Table 46 (given according to their position(s) on the amino acid sequence in the first column; the second column indicates whether the glycosylation site is present in the variant protein; and the last column indicates whether the position is different on the variant protein).

TABLE 46 Glycosylation site(s) Position(s) on known amino acid sequence Present in variant protein? 106 no

Variant protein T46984_PEA_(—)1_P46 (SEQ ID NO: 398) is encoded by the following transcript(s): T46984_PEA_(—)1_T35 (SEQ ID NO: 325), for which the sequence(s) is/are given at the end of the application. The coding portion of transcript T46984_PEA_(—)1_T35 (SEQ ID NO: 325) is shown in bold; this coding portion starts at position 316 and ends at position 567. The transcript also has the following SNPs as listed in Table 47 (given according to their position on the nucleotide sequence, with the alternative nucleic acid listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein T46984_PEA_(—)1_P46 (SEQ ID NO: 398) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 47 Nucleic acid SNPs SNP position on nucleotide Previously known sequence Alternative nucleic acid SNP? 28 G -> C No 173 G -> C Yes 256 C -> T Yes 274 G -> C Yes 325 C -> No 389 C -> G Yes 572 C -> T Yes 585 -> G No 613 C -> No 625 -> G No 730 G -> A No 776 A -> No 776 A -> G No 796 A -> C No 804 G -> A Yes 915 A -> No 915 A -> C No 948 A -> G No 948 A -> T No 982 T -> G No 1019 A -> C No 1021 C -> G No 1031 G -> A No 1104 G -> T Yes 1316 A -> C No 1346 T -> No 1346 T -> C No 1388 T -> No 1421 A -> C No 1421 A -> T No 1652 C -> No 1652 C -> A No 1659 C -> No 1663 G -> No 1848 C -> A No 1868 T -> C Yes 1962 C -> T No 1995 A -> C No 2007 A -> T No 2022 G -> A Yes 2028 -> A No 2037 A -> G No 2062 T -> G No 2076 T -> A No 2076 T -> G No 2176 -> C No 2185 T -> No 2244 A -> C No

As noted above, cluster T46984 features 49 segment(s), which were listed in Table 2 above and for which the sequence(s) are given at the end of the application. These segment(s) are portions of nucleic acid sequence(s) which are described herein separately because they are of particular interest. A description of each segment according to the present invention is now provided.

Segment cluster T46984_PEA_(—)1_node_(—)2 (SEQ ID NO: 335) according to the present invention is supported by 240 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): T46984_PEA_(—)1_T2 (SEQ ID NO: 314) (SEQ ID NO: 314), T46984_PEA_(—)1_T3 (SEQ ID NO: 315), T46984_PEA_(—)1_T12 (SEQ ID NO: 316), T46984_PEA_(—)1_T13 (SEQ ID NO: 317), T46984_PEA_(—)1_T14 (SEQ ID NO: 318), T46984_PEA_(—)1_T15 (SEQ ID NO: 319), T46984_PEA_(—)1_T19 (SEQ ID NO: 320), T46984_PEA_(—)1_T23 (SEQ ID NO: 321), T46984_PEA_(—)1_T32 (SEQ ID NO: 323), T46984_PEA_(—)1_T34 (SEQ ID NO: 324), T46984_PEA_(—)1_T35 (SEQ ID NO: 325), T46984_PEA_(—)1_T40 (SEQ ID NO: 326), T46984_PEA_(—)1_T42 (SEQ ID NO: 327), T46984_PEA_(—)1_T43 (SEQ ID NO: 328), T46984_PEA_(—)1_T47 (SEQ ID NO: 330) and T46984_PEA_(—)1_T48 (SEQ ID NO: 331). Table 48 below describes the starting and ending position of this segment on each transcript.

TABLE 48 Segment location on transcripts Segment Segment starting ending Transcript name position position T46984_PEA_1_T2 1 328 (SEQ ID NO:314) (SEQ ID NO:314) T46984_PEA_1_T3 1 328 (SEQ ID NO:315) T46984_PEA_1_T12 1 328 (SEQ ID NO:316) T46984_PEA_1_T13 1 328 (SEQ ID NO:317) T46984_PEA_1_T14 1 328 (SEQ ID NO:318) T46984_PEA_1_T15 1 328 (SEQ ID NO:319) T46984_PEA_1_T19 1 328 (SEQ ID NO:320) T46984_PEA_1_T23 1 328 (SEQ ID NO:321) T46984_PEA_1_T32 1 328 (SEQ ID NO:323) T46984_PEA_1_T34 1 328 (SEQ ID NO:324) 146984_PEA_1_T35 1 328 (SEQ ID NO:325) T46984_PEA_1_T40 1 328 (SEQ ID NO:326) T46984_PEA_1_T42 1 328 (SEQ ID NO:327) T46984_PEA_1_T43 1 328 (SEQ ID NO:328) T46984_PEA_1_T47 1 328 (SEQ ID NO:330) T46984_PEA_1_T48 1 328 (SEQ ID NO:331)

Segment cluster T46984_PEA_(—)1_node_(—)4 (SEQ ID NO: 336) according to the present invention is supported by 321 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): T46984_PEA_(—)1_T2 (SEQ ID NO: 314) (SEQ ID NO: 314), T46984_PEA_(—)1_T3 (SEQ ID NO: 315), T46984_PEA_(—)1_T12 (SEQ ID NO: 316), T46984_PEA_(—)1_T13 (SEQ ID NO: 317), T46984_PEA_(—)1_T14 (SEQ ID NO: 318), T46984_PEA_(—)1_T15 (SEQ ID NO: 319), T46984_PEA_(—)1_T19 (SEQ ID NO: 320), T46984_PEA_(—)1_T23 (SEQ ID NO: 321), T46984_PEA_(—)1_T32 (SEQ ID NO: 323), T46984_PEA_(—)1_T34 (SEQ ID NO: 324), T46984_PEA_(—)1_T35 (SEQ ID NO: 325), T46984_PEA_(—)1_T40 (SEQ ID NO: 326), T46984_PEA_(—)1_T42 (SEQ ID NO: 327), T46984_PEA_(—)1_T43 (SEQ ID NO: 328), T46984_PEA_(—)1_T47 (SEQ ID NO: 330) and T46984_PEA_(—)1_T48 (SEQ ID NO: 331). Table 49 below describes the starting and ending position of this segment on each transcript.

TABLE 49 Segment location on transcripts Segment Segment Transcript name starting position ending position T46984_PEA_1_T2 329 522 (SEQ ID NO: 314) (SEQ ID NO: 314) T46984_PEA_1_T3 329 522 (SEQ ID NO: 315) T46984_PEA_1_T12 329 522 (SEQ ID NO: 316) T46984_PEA_1_T13 329 522 (SEQ ID NO: 317) T46984_PEA_1_T14 329 522 (SEQ ID NO: 318) T46984_PEA_1_T15 329 522 (SEQ ID NO: 319) T46984_PEA_1_T19 329 522 (SEQ ID NO: 320) T46984_PEA_1_T23 329 522 (SEQ ID NO: 321) T46984_PEA_1_T32 329 522 (SEQ ID NO: 323) T46984_PEA_1_T34 329 522 (SEQ ID NO: 324) T46984_PEA_1_T35 329 522 (SEQ ID NO: 325) T46984_PEA_1_T40 329 522 (SEQ ID NO: 326) T46984_PEA_1_T42 329 522 (SEQ ID NO: 327) T46984_PEA_1_T43 329 522 (SEQ ID NO: 328) T46984_PEA_1_T47 329 522 (SEQ ID NO: 330) T46984_PEA_1_T48 329 522 (SEQ ID NO: 331)

Segment cluster T46984_PEA_(—)1_node_(—)6 (SEQ ID NO: 337) according to the present invention is supported by 3 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): T46984_PEA_(—)1_T27 (SEQ ID NO: 322). Table 50 below describes the starting and ending position of this segment on each transcript.

TABLE 50 Segment location on transcripts Segment Segment starting ending Transcript name position position T46984_PEA_1_T27 1 340 (SEQ ID NO:322)

Segment cluster T46984_PEA_(—)1_node_(—)12 (SEQ ID NO: 338) according to the present invention is supported by 262 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): T46984_PEA_(—)1_T2 (SEQ ID NO: 314) (SEQ ID NO: 314), T46984_PEA_(—)1_T3 (SEQ ID NO: 315), T46984_PEA_(—)1_T12 (SEQ ID NO: 316), T46984_PEA_(—)1_T13 (SEQ ID NO: 317), T46984_PEA_(—)1_T14 (SEQ ID NO: 318), T46984_PEA_(—)1_T15 (SEQ ID NO: 319), T46984_PEA_(—)1_T19 (SEQ ID NO: 320), T46984_PEA_(—)1_T23 (SEQ ID NO: 321), T46984_PEA_(—)1_T27 (SEQ ID NO: 322), T46984_PEA_(—)1_T34 (SEQ ID NO: 324), T46984_PEA_(—)1_T40 (SEQ ID NO: 326), T46984_PEA_(—)1_T42 (SEQ ID NO: 327), T46984_PEA_(—)1_T43 (SEQ ID NO: 328), T46984_PEA_(—)1_T47 (SEQ ID NO: 330) and T46984_PEA_(—)1_T48 (SEQ ID NO: 331). Table 51 below describes the starting and ending position of this segment on each transcript.

TABLE 51 Segment location on transcripts Segment Segment starting ending Transcript name position position T46984_PEA_1_T2 619 751 (SEQ ID NO:314) T46984_PEA_1_T3 619 751 (SEQ ID NO:315) T46984_PEA_1_T12 619 751 (SEQ ID NO:316) T46984_PEA_1_T13 619 751 (SEQ ID NO:317) T46984_PEA_1_T14 619 751 (SEQ ID NO:318) T46984_PEA_1_T15 619 751 (SEQ ID NO:319) T46984_PEA_1_T19 619 751 (SEQ ID NO:320) T46984_PEA_1_T23 619 751 (SEQ ID NO:321) T46984_PEA_1_T27 437 569 (SEQ ID NO:322) T46984_PEA_1_T34 619 751 (SEQ ID NO:324) T46984_PEA_1_T40 619 751 (SEQ ID NO:326) T46984_PEA_1_T42 619 751 (SEQ ID NO:327) T46984_PEA_1_T43 619 751 (SEQ ID NO:328) T46984_PEA_1_T47 619 751 (SEQ ID NO:330) T46984_PEA_1_T48 619 751 (SEQ ID NO:331)

Segment cluster T46984_PEA_(—)1_node_(—)14 (SEQ ID NO: 339) according to the present invention is supported by 2 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): T46984_PEA_(—)1_T48 (SEQ ID NO: 331). Table 52 below describes the starting and ending position of this segment on each transcript.

TABLE 52 Segment location on transcripts Segment Segment starting ending Transcript name position position T46984_PEA_1_T48 795 1718 (SEQ ID NO:331)

Segment cluster T46984_PEA_(—)1_node_(—)25 (SEQ ID NO: 340) according to the present invention is supported by 257 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): T46984_PEA_(—)1_T2 (SEQ ID NO: 314), T46984_PEA_(—)1_T3 (SEQ ID NO: 315), T46984_PEA_(—)1_T12 (SEQ ID NO: 316), T46984_PEA_(—)1_T13 (SEQ ID NO: 317), T46984_PEA_(—)1_T14 (SEQ ID NO: 318), T46984_PEA_(—)1_T15 (SEQ ID NO: 319), T46984_PEA_(—)1_T19 (SEQ ID NO: 320), T46984_PEA_(—)1_T23 (SEQ ID NO: 321), T46984_PEA_(—)1_T27 (SEQ ID NO: 322), T46984_PEA_(—)1_T32 (SEQ ID NO: 323), T46984_PEA_(—)1_T34 (SEQ ID NO: 324), T46984_PEA_(—)1_T35 (SEQ ID NO: 325), T46984_PEA_(—)1_T40 (SEQ ID NO: 326), T46984_PEA_(—)1_T42 (SEQ ID NO: 327) and T46984_PEA_(—)1_T43 (SEQ ID NO: 328). Table 53 below describes the starting and ending position of this segment on each transcript.

TABLE 53 Segment location on transcripts Segment Segment Transcript name starting position ending position T46984_PEA 1_T2 1006 1171 (SEQ ID NO: 314) T46984_PEA_1_T3 1006 1171 (SEQ ID NO: 315) T46984_PEA_1_T12 1006 1171 (SEQ ID NO: 316) T46984_PEA_1_T13 1006 1171 (SEQ ID NO: 317) T46984_PEA_1_T14 1006 1171 (SEQ ID NO: 318) T46984_PEA_1_T15 1006 1171 (SEQ ID NO: 319) T46984_PEA_1_T19 1006 1171 (SEQ ID NO: 320) T46984_PEA_1_T23 1006 1171 (SEQ ID NO: 321) T46984_PEA_1_T27 824 989 (SEQ ID NO: 322) T46984_PEA_1_T32 830 995 (SEQ ID NO: 323) T46984_PEA_1_T34 1006 1171 (SEQ ID NO: 324) T46984_PEA_1_T35 734 899 (SEQ ID NO: 325) T46984_PEA_1_T40 1006 1171 (SEQ ID NO: 326) T46984_PEA_1_T42 1006 1171 (SEQ ID NO: 327) T46984_PEA_1_T43 1006 1171 (SEQ ID NO: 328)

Segment cluster T46984_PEA_(—)1_node_(—)29 (SEQ ID NO: 341) according to the present invention is supported by 1 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): T46984_PEA_(—)1_T42 (SEQ ID NO: 327). Table 54 below describes the starting and ending position of this segment on each transcript.

TABLE 54 Segment location on transcripts Segment Segment Transcript name starting position ending position T46984_PEA_1_T42 1302 1501 (SEQ ID NO: 327)

Segment cluster T46984_PEA_(—)1_node_(—)34 (SEQ ID NO: 342) according to the present invention is supported by 4 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): T46984_PEA_(—)1_T40 (SEQ ID NO: 326). Table 55 below describes the starting and ending position of this segment on each transcript.

TABLE 55 Segment location on transcripts Segment Segment Transcript name starting position ending position T46984_PEA_1_T40 1408 1717 (SEQ ID NO: 326)

Segment cluster T46984_PEA_(—)1_node_(—)46 (SEQ ID NO: 343) according to the present invention is supported by 1 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): T46984_PEA_(—)1_T46 (SEQ ID NO: 329). Table 56 below describes the starting and ending position of this segment on each transcript.

TABLE 56 Segment location on transcripts Segment Segment Transcript name starting position ending position T46984_PEA_1_T46 1 306 (SEQ ID NO: 329)

Segment cluster T46984_PEA_(—)1_node_(—)47 (SEQ ID NO: 344) according to the present invention is supported by 5 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): T46984_PEA_(—)1_T3 (SEQ ID NO: 315), T46984_PEA_(—)1_T19 (SEQ ID NO: 320) and T46984_PEA_(—)1_T46 (SEQ ID NO: 329). Table 57 below describes the starting and ending position of this segment on each transcript.

TABLE 57 Segment location on transcripts Segment Segment Transcript name starting position ending position T46984_PEA_1_T3 1615 2242 (SEQ ID NO: 315) T46984_PEA_1_T19 1615 2242 (SEQ ID NO: 320) T46984_PEA_1_T46 307 934 (SEQ ID NO: 329)

Segment cluster T46984_PEA_(—)1_node_(—)52 (SEQ ID NO: 345) according to the present invention is supported by 29 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): T46984_PEA_(—)1_T2 (SEQ ID NO: 314), T46984_PEA_(—)1_T19 (SEQ ID NO: 320) and T46984_PEA_(—)1_T23 (SEQ ID NO: 321). Table 58 below describes the starting and ending position of this segment on each transcript.

TABLE 58 Segment location on transcripts Segment Segment Transcript name starting position ending position T46984_PEA_1_T2 1838 2904 (SEQ ID NO: 314) T46984_PEA_1_T19 2466 3532 (SEQ ID NO: 320) T46984_PEA_1_T23 1838 2904 (SEQ ID NO: 321)

Segment cluster T46984_PEA_(—)1_node_(—)6 (SEQ ID NO: 337)5 according to the present invention is supported by 2 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): T46984_PEA_(—)1_T51 (SEQ ID NO: 332). Table 59 below describes the starting and ending position of this segment on each transcript.

TABLE 59 Segment location on transcripts Segment Segment Transcript name starting position ending position T46984_PEA_1_T51 1 348 (SEQ ID NO: 332)

Segment cluster T46984_PEA_(—)1_node_(—)69 (SEQ ID NO: 347) according to the present invention is supported by 8 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): T46984_PEA_(—)1_T52 (SEQ ID NO: 333) and T46984_PEA_(—)1_T54 (SEQ ID NO: 334). Table 60 below describes the starting and ending position of this segment on each transcript.

TABLE 60 Segment location on transcripts Segment Segment Transcript name starting position ending position T46984_PEA_1_T52 1 927 (SEQ ID NO: 333) T46984_PEA_1_T54 1 927 (SEQ ID NO: 334)

Segment cluster T46984_PEA_(—)1_node_(—)75 (SEQ ID NO: 348) according to the present invention is supported by 5 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): T46984_PEA_(—)1_T14 (SEQ ID NO: 318). Table 61 below describes the starting and ending position of this segment on each transcript.

TABLE 61 Segment location on transcripts Segment Segment Transcript name starting position ending position T46984_PEA_1_T14 2199 3529 (SEQ ID NO: 318)

Segment cluster T46984_PEA_(—)1_node_(—)86 (SEQ ID NO: 349) according to the present invention is supported by 314 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): T46984_PEA_(—)1_T2 (SEQ ID NO: 314), T46984_PEA_(—)1_T3 (SEQ ID NO: 315), T46984_PEA_(—)1_T12 (SEQ ID NO: 316), T46984_PEA_(—)1_T13 (SEQ ID NO: 317), T46984_PEA_(—)1_T15 (SEQ ID NO: 319), T46984_PEA_(—)1_T19 (SEQ ID NO: 320), T46984_PEA_(—)1_T23 (SEQ ID NO: 321), T46984_PEA_(—)1_T27 (SEQ ID NO: 322), T46984_PEA_(—)1_T32 (SEQ ID NO: 323), T46984_PEA_(—)1_T34 (SEQ ID NO: 324), T46984_PEA_(—)1_T35 (SEQ ID NO: 325), T46984_PEA_(—)1_T43 (SEQ ID NO: 328), T46984_PEA_(—)1_T46 (SEQ ID NO: 329), T46984_PEA_(—)1_T47 (SEQ ID NO: 330), T46984_PEA_(—)1_T51 (SEQ ID NO: 332), T46984_PEA_(—)1_T52 (SEQ ID NO: 333) and T46984_PEA_(—)1_T54 (SEQ ID NO: 334). Table 62 below describes the starting and ending position of this segment on each transcript.

TABLE 62 Segment location on transcripts Segment Segment Transcript name starting position ending position T46984_PEA_1_T2 3492 3750 (SEQ ID NO: 314) T46984_PEA_1_T3 2886 3144 (SEQ ID NO: 315) T46984_PEA_1_T12 2286 2544 (SEQ ID NO: 316) T46984_PEA_1_T13 2317 2575 (SEQ ID NO: 317) T46984_PEA_1_T15 2175 2433 (SEQ ID NO: 319) T46984_PEA_1_T19 4120 4378 (SEQ ID NO: 320) T46984_PEA_1_T23 3396 3654 (SEQ ID NO: 321) T46984_PEA_1_T27 2076 2334 (SEQ ID NO: 322) T46984_PEA_1_T32 2082 2340 (SEQ ID NO: 323) T46984_PEA_1_T34 1828 2086 (SEQ ID NO: 324) T46984_PEA_1_T35 1986 2244 (SEQ ID NO: 325) T46984_PEA_1_T43 1264 1522 (SEQ ID NO: 328) T46984_PEA_1_T46 1578 1836 (SEQ ID NO: 329) T46984_PEA_1_T47 1017 1275 (SEQ ID NO: 330) T46984_PEA_1_T51 614 872 (SEQ ID NO: 332) T46984_PEA_1_T52 1117 1375 (SEQ ID NO: 333) T46984_PEA_1_T54 1117 1602 (SEQ ID NO: 334)

According to an optional embodiment of the present invention, short segments related to the above cluster are also provided. These segments are up to about 120 bp in length, and so are included in a separate description.

Segment cluster T46984_PEA_(—)1_node_(—)9 (SEQ ID NO: 350) according to the present invention is supported by 304 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): T46984_PEA_(—)1_T2 (SEQ ID NO: 314), T46984_PEA_(—)1_T3 (SEQ ID NO: 315), T46984_PEA_(—)1_T12 (SEQ ID NO: 316), T46984_PEA_(—)1_T13 (SEQ ID NO: 317), T46984_PEA_(—)1_T14 (SEQ ID NO: 318), T46984_PEA_(—)1_T15 (SEQ ID NO: 319), T46984_PEA_(—)1_T19 (SEQ ID NO: 320), T46984_PEA_(—)1_T23 (SEQ ID NO: 321), T46984_PEA_(—)1_T27 (SEQ ID NO: 322), T46984_PEA_(—)1_T32 (SEQ ID NO: 323), T46984_PEA_(—)1_T34 (SEQ ID NO: 324), T46984_PEA_(—)1_T40 (SEQ ID NO: 326), T46984_PEA_(—)1_T42 (SEQ ID NO: 327), T46984_PEA_(—)1_T43 (SEQ ID NO: 328), T46984_PEA_(—)1_T47 (SEQ ID NO: 330) and T46984_PEA_(—)1_T48 (SEQ ID NO: 331). Table 63 below describes the starting and ending position of this segment on each transcript.

TABLE 63 Segment location on transcripts Segment Segment Transcript name starting position ending position T46984_PEA_1_T2 523 618 (SEQ ID NO: 314) T46984_PEA_1_T3 523 618 (SEQ ID NO: 315) T46984_PEA_1_T12 523 618 (SEQ ID NO: 316) T46984_PEA_1_T13 523 618 (SEQ ID NO: 317) T46984_PEA_1_T14 523 618 (SEQ ID NO: 318) T46984_PEA_1_T15 523 618 (SEQ ID NO: 319) T46984_PEA_1_T19 523 618 (SEQ ID NO: 320) T46984_PEA_1_T23 523 618 (SEQ ID NO: 321) T46984_PEA_1_T27 341 436 (SEQ ID NO: 322) T46984_PEA_1_T32 523 618 (SEQ ID NO: 323) T46984_PEA_1_T34 523 618 (SEQ ID NO: 324) T46984_PEA_1_T40 523 618 (SEQ ID NO: 326) T46984_PEA_1_T42 523 618 (SEQ ID NO: 327) T46984_PEA_1_T43 523 618 (SEQ ID NO: 328) T46984_PEA_1_T47 523 618 (SEQ ID NO: 330) T46984_PEA_1_T48 523 618 (SEQ ID NO: 331)

Segment cluster T46984_PEA_(—)1_node_(—)13 (SEQ ID NO: 351) according to the present invention is supported by 232 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): T46984_PEA_(—)1_T2 (SEQ ID NO: 314), T46984_PEA_(—)1_T3 (SEQ ID NO: 315), T46984_PEA_(—)1_T12 (SEQ ID NO: 316), T46984_PEA_(—)1_T13 (SEQ ID NO: 317), T46984_PEA_(—)1_T14 (SEQ ID NO: 318), T46984_PEA_(—)1_T15 (SEQ ID NO: 319), T46984_PEA_(—)1_T19 (SEQ ID NO: 320), T46984_PEA_(—)1_T23 (SEQ ID NO: 321), T46984_PEA_(—)1_T27 (SEQ ID NO: 322), T46984_PEA_(—)1_T34 (SEQ ID NO: 324), T46984_PEA_(—)1_T40 (SEQ ID NO: 326), T46984_PEA_(—)1_T42 (SEQ ID NO: 327), T46984_PEA_(—)1_T43 (SEQ ID NO: 328) and T46984_PEA_(—)1_T48 (SEQ ID NO: 331). Table 64 below describes the starting and ending position of this segment on each transcript.

TABLE 64 Segment location on transcripts Segment Segment Transcript name starting position ending position T46984_PEA_1_T2 752 794 (SEQ ID NO: 314) T46984_PEA_1_T3 752 794 (SEQ ID NO: 315) T46984_PEA_1_T12 752 794 (SEQ ID NO: 316) T46984_PEA_1_T13 752 794 (SEQ ID NO: 317) T46984_PEA_1_T14 752 794 (SEQ ID NO: 318) T46984_PEA_1_T15 752 794 (SEQ ID NO: 319) T46984_PEA_1_T19 752 794 (SEQ ID NO: 320) T46984_PEA_1_T23 752 794 (SEQ ID NO: 321) T46984_PEA_1_T27 570 612 (SEQ ID NO: 322) T46984_PEA_1_T34 752 794 (SEQ ID NO: 324) T46984_PEA_1_T40 752 794 (SEQ ID NO: 326) T46984_PEA_1_T42 752 794 (SEQ ID NO: 327) T46984_PEA_1_T43 752 794 (SEQ ID NO: 328) T46984_PEA_1_T48 752 794 (SEQ ID NO: 331)

Segment cluster T46984_PEA_(—)1_node_(—)19 (SEQ ID NO: 352) according to the present invention is supported by 237 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): T46984_PEA_(—)1_T2 (SEQ ID NO: 314), T46984_PEA_(—)1_T3 (SEQ ID NO: 315), T46984_PEA_(—)1_T12 (SEQ ID NO: 316), T46984_PEA_(—)1_T13 (SEQ ID NO: 317), T46984_PEA_(—)1_T14 (SEQ ID NO: 318), T46984_PEA_(—)1_T15 (SEQ ID NO: 319), T46984_PEA_(—)1_T19 (SEQ ID NO: 320), T46984_PEA_(—)1_T23 (SEQ ID NO: 321), T46984_PEA_(—)1_T27 (SEQ ID NO: 322), T46984_PEA_(—)1_T32 (SEQ ID NO: 323), T46984_PEA_(—)1_T34 (SEQ ID NO: 324), T46984_PEA_(—)1_T35 (SEQ ID NO: 325), T46984_PEA_(—)1_T40 (SEQ ID NO: 326), T46984_PEA_(—)1_T42 (SEQ ID NO: 327) and T46984_PEA_(—)1_T43 (SEQ ID NO: 328). Table 65 below describes the starting and ending position of this segment on each transcript.

TABLE 65 Segment location on transcripts Segment Segment Transcript name starting position ending position T46984_PEA_1_T2 795 870 (SEQ ID NO: 314) T46984_PEA_1_T3 795 870 (SEQ ID NO: 315) T46984_PEA_1_T12 795 870 (SEQ ID NO: 316) T46984_PEA_1_T13 795 870 (SEQ ID NO: 317) T46984_PEA_1_T14 795 870 (SEQ ID NO: 318) T46984_PEA_1_T15 795 870 (SEQ ID NO: 319) T46984_PEA_1_T19 795 870 (SEQ ID NO: 320) T46984_PEA_1_T23 795 870 (SEQ ID NO: 321) T46984_PEA_1_T27 613 688 (SEQ ID NO: 322) T46984_PEA_1_T32 619 694 (SEQ ID NO: 323) T46984_PEA_1_T34 795 870 (SEQ ID NO: 324) T46984_PEA_1_T35 523 598 (SEQ ID NO: 325) T46984_PEA_1_T40 795 870 (SEQ ID NO: 326) T46984_PEA_1_T42 795 870 (SEQ ID NO: 327) T46984_PEA_1_T43 795 870 (SEQ ID NO: 328)

Segment cluster T46984_PEA_(—)1_node_(—)21 (SEQ ID NO: 353) according to the present invention is supported by 242 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): T46984_PEA_(—)1_T2 (SEQ ID NO: 314), T46984_PEA_(—)1_T3 (SEQ ID NO: 315), T46984_PEA_(—)1_T12 (SEQ ID NO: 316), T46984_PEA_(—)1_T13 (SEQ ID NO: 317), T46984_PEA_(—)1_T14 (SEQ ID NO: 318), T46984_PEA_(—)1_T15 (SEQ ID NO: 319), T46984_PEA_(—)1_T19 (SEQ ID NO: 320), T46984_PEA_(—)1_T23 (SEQ ID NO: 321), T46984_PEA_(—)1_T27 (SEQ ID NO: 322), T46984_PEA_(—)1_T32 (SEQ ID NO: 323), T46984_PEA_(—)1_T34 (SEQ ID NO: 324), T46984_PEA_(—)1_T35 (SEQ ID NO: 325), T46984_PEA_(—)1_T40 (SEQ ID NO: 326), T46984_PEA_(—)1_T42 (SEQ ID NO: 327) and T46984_PEA_(—)1_T43 (SEQ ID NO: 328). Table 66 below describes the starting and ending position of this segment on each transcript.

TABLE 66 Segment location on transcripts Segment Segment Transcript name starting position ending position T46984_PEA_1_T2 871 975 (SEQ ID NO: 314) T46984_PEA_1_T3 871 975 (SEQ ID NO: 315) T46984_PEA_1_T12 871 975 (SEQ ID NO: 316) T46984_PEA_1_T13 871 975 (SEQ ID NO: 317) T46984_PEA_1_T14 871 975 (SEQ ID NO: 318) T46984_PEA_1_T15 871 975 (SEQ ID NO: 319) T46984_PEA_1_T19 871 975 (SEQ ID NO: 320) T46984_PEA_1_T23 871 975 (SEQ ID NO: 321) T46984_PEA_1_T27 689 793 (SEQ ID NO: 322) T46984_PEA_1_T32 695 799 (SEQ ID NO: 323) T46984_PEA_1_T34 871 975 (SEQ ID NO: 324) T46984_PEA_1_T35 599 703 (SEQ ID NO: 325) T46984_PEA_1_T40 871 975 (SEQ ID NO: 326) T46984_PEA_1_T42 871 975 (SEQ ID NO: 327) T46984_PEA_1_T43 871 975 (SEQ ID NO: 328)

Segment cluster T46984_PEA_(—)1_node_(—)22 (SEQ ID NO: 354) according to the present invention is supported by 205 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): T46984_PEA_(—)1_T2 (SEQ ID NO: 314), T46984_PEA_(—)1_T3 (SEQ ID NO: 315), T46984_PEA_(—)1_T12 (SEQ ID NO: 316), T46984_PEA_(—)1_T13 (SEQ ID NO: 317), T46984_PEA_(—)1_T14 (SEQ ID NO: 318), T46984_PEA_(—)1_T15 (SEQ ID NO: 319), T46984_PEA_(—)1_T19 (SEQ ID NO: 320), T46984_PEA_(—)1_T23 (SEQ ID NO: 321), T46984_PEA_(—)1_T27 (SEQ ID NO: 322), T46984_PEA_(—)1_T32 (SEQ ID NO: 323), T46984_PEA_(—)1_T34 (SEQ ID NO: 324), T46984_PEA_(—)1_T35 (SEQ ID NO: 325), T46984_PEA_(—)1_T40 (SEQ ID NO: 326), T46984_PEA_(—)1_T42 (SEQ ID NO: 327) and T46984_PEA_(—)1_T43 (SEQ ID NO: 328). Table 67 below describes the starting and ending position of this segment on each transcript.

TABLE 67 Segment location on transcripts Segment Segment Transcript name starting position ending position T46984_PEA_1_T2 (SEQ ID NO: 976 1005 314) T46984_PEA_1_T3 (SEQ ID NO: 976 1005 315) T46984_PEA_1_T12 (SEQ ID NO: 976 1005 316) T46984_PEA_1_T13 (SEQ ID NO: 976 1005 317) T46984_PEA_1_T14 (SEQ ID NO: 976 1005 318) T46984_PEA_1_T15 (SEQ ID NO: 976 1005 319) T46984_PEA_1_T19 (SEQ ID NO: 976 1005 320) T46984_PEA_1_T23 (SEQ ID NO: 976 1005 321) T46984_PEA_1_T27 (SEQ ID NO: 794 823 322) T46984_PEA_1_T32 (SEQ ID NO: 800 829 323) T46984_PEA_1_T34 (SEQ ID NO: 976 1005 324) T46984_PEA_1_T35 (SEQ ID NO: 704 733 325) T46984_PEA_1_T40 (SEQ ID NO: 976 1005 326) T46984_PEA_1_T42 (SEQ ID NO: 976 1005 327) T46984_PEA_1_T43 (SEQ ID NO: 976 1005 328)

Segment cluster T46984_PEA_(—)1_node_(—)26 (SEQ ID NO: 355) according to the present invention can be found in the following transcript(s): T46984_PEA_(—)1_T2 (SEQ ID NO: 314), T46984_PEA_(—)1_T3 (SEQ ID NO: 315), T46984_PEA_(—)1_T12 (SEQ ID NO: 316), T46984_PEA_(—)1_T13 (SEQ ID NO: 317), T46984_PEA_(—)1_T14 (SEQ ID NO: 318), T46984_PEA_(—)1_T15 (SEQ ID NO: 319), T46984_PEA_(—)1_T19 (SEQ ID NO: 320), T46984_PEA_(—)1_T23 (SEQ ID NO: 321), T46984_PEA_(—)1_T27 (SEQ ID NO: 322), T46984_PEA_(—)1_T32 (SEQ ID NO: 323), T46984_PEA_(—)1_T34 (SEQ ID NO: 324), T46984_PEA_(—)1_T35 (SEQ ID NO: 325), T46984_PEA_(—)1_T40 (SEQ ID NO: 326) and T46984_PEA_(—)1_T42 (SEQ ID NO: 327). Table 68 below describes the starting and ending position of this segment on each transcript.

TABLE 68 Segment location on transcripts Segment Segment Transcript name starting position ending position T46984_PEA_1_T2 1172 1182 (SEQ ID NO: 314) T46984_PEA_1_T3 1172 1182 (SEQ ID NO: 315) T46984_PEA_1_T12 1172 1182 (SEQ ID NO: 316) T46984_PEA_1_T13 1172 1182 (SEQ ID NO: 317) T46984_PEA_1_T14 1172 1182 (SEQ ID NO: 318) T46984_PEA_1_T15 1172 1182 (SEQ ID NO: 319) T46984_PEA_1_T19 1172 1182 (SEQ ID NO: 320) T46984_PEA_1_T23 1172 1182 (SEQ ID NO: 321) T46984_PEA_1_T27 990 1000 (SEQ ID NO: 322) T46984_PEA_1_T32 996 1006 (SEQ ID NO: 323) T46984_PEA_1_T34 1172 1182 (SEQ ID NO: 324) T46984_PEA_1_T35 900 910 (SEQ ID NO: 325) T46984_PEA_1_T40 1172 1182 (SEQ ID NO: 326) T46984_PEA_1_T42 1172 1182 (SEQ ID NO: 327)

Segment cluster T46984_PEA_(—)1_node_(—)28 (SEQ ID NO: 356) according to the present invention is supported by 242 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): T46984_PEA_(—)1_T2 (SEQ ID NO: 314), T46984_PEA_(—)1_T3 (SEQ ID NO: 315), T46984_PEA_(—)1_T12 (SEQ ID NO: 316), T46984_PEA_(—)1_T13 (SEQ ID NO: 317), T46984_PEA_(—)1_T14 (SEQ ID NO: 318), T46984_PEA_(—)1_T15 (SEQ ID NO: 319), T46984_PEA_(—)1_T19 (SEQ ID NO: 320), T46984_PEA_(—)1_T23 (SEQ ID NO: 321), T46984_PEA_(—)1_T27 (SEQ ID NO: 322), T46984_PEA_(—)1_T32 (SEQ ID NO: 323), T46984_PEA_(—)1_T34 (SEQ ID NO: 324), T46984_PEA_(—)1_T35 (SEQ ID NO: 325), T46984_PEA_(—)1_T40 (SEQ ID NO: 326) and T46984_PEA_(—)1_T42 (SEQ ID NO: 327). Table 69 below describes the starting and ending position of this segment on each transcript.

TABLE 69 Segment location on transcripts Segment Segment Transcript name starting position ending position T46984_PEA_1_T2 1183 1301 (SEQ ID NO: 314) T46984_PEA_1_T3 1183 1301 (SEQ ID NO: 315) T46984_PEA_1_T12 1183 1301 (SEQ ID NO: 316) T46984_PEA_1_T13 1183 1301 (SEQ ID NO: 317) T46984_PEA_1_T14 1183 1301 (SEQ ID NO: 318) T46984_PEA_1_T15 1183 1301 (SEQ ID NO: 319) T46984_PEA_1_T19 1183 1301 (SEQ ID NO: 320) T46984_PEA_1_T23 1183 1301 (SEQ ID NO: 321) T46984_PEA_1_T27 1001 1119 (SEQ ID NO: 322) T46984_PEA_1_T32 1007 1125 (SEQ ID NO: 323) 146984_PEA_1_T34 1183 1301 (SEQ ID NO: 324) T46984_PEA_1_T35 911 1029 (SEQ ID NO: 325) T46984_PEA_1_T40 1183 1301 (SEQ ID NO: 326) T46984_PEA_1_T42 1183 1301 (SEQ ID NO: 327)

Segment cluster T46984_PEA_(—)1_node_(—)31 (SEQ ID NO: 357) according to the present invention is supported by 207 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): T46984_PEA_(—)1_T2 (SEQ ID NO: 314), T46984_PEA_(—)1_T3 (SEQ ID NO: 315), T46984_PEA_(—)1_T12 (SEQ ID NO: 316), T46984_PEA_(—)1_T13 (SEQ ID NO: 317), T46984_PEA_(—)1_T14 (SEQ ID NO: 318), T46984_PEA_(—)1_T15 (SEQ ID NO: 319), T46984_PEA_(—)1_T19 (SEQ ID NO: 320), T46984_PEA_(—)1_T23 (SEQ ID NO: 321), T46984_PEA_(—)1_T27 (SEQ ID NO: 322), T46984_PEA_(—)1_T32 (SEQ ID NO: 323), T46984_PEA_(—)1_T34 (SEQ ID NO: 324), T46984_PEA_(—)1_T35 (SEQ ID NO: 325) and T46984_PEA_(—)1_T40 (SEQ ID NO: 326). Table 70 below describes the starting and ending position of this segment on each transcript.

TABLE 70 Segment location on transcripts Segment Segment Transcript name starting position ending position T46984_PEA_1_T2 1302 1329 (SEQ ID NO: 314) T46984_PEA_1_T3 1302 1329 (SEQ ID NO: 315) T46984_PEA_1_T12 1302 1329 (SEQ ID NO: 316) T46984_PEA_1_T13 1302 1329 (SEQ ID NO: 317) T46984_PEA_1_T14 1302 1329 (SEQ ID NO: 318) T46984_PEA_1_T15 1302 1329 (SEQ ID NO: 319) T46984_PEA_1_T19 1302 1329 (SEQ ID NO: 320) T46984_PEA_1_T23 1302 1329 (SEQ ID NO: 321) T46984_PEA_1_T27 1120 1147 (SEQ ID NO: 322) T46984_PEA_1_T32 1126 1153 (SEQ ID NO: 323) T46984_PEA_1_T34 1302 1329 (SEQ ID NO: 324) T46984_PEA_1_T35 1030 1057 (SEQ ID NO: 325) T46984_PEA_1_T40 1302 1329 (SEQ ID NO: 326)

Segment cluster T46984_PEA_(—)1_node_(—)32 (SEQ ID NO: 358) according to the present invention is supported by 226 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): T46984_PEA_(—)1_T2 (SEQ ID NO: 314), T46984_PEA_(—)1_T3 (SEQ ID NO: 315), T46984_PEA_(—)1_T12 (SEQ ID NO: 316), T46984_PEA_(—)1_T13 (SEQ ID NO: 317), T46984_PEA_(—)1_T14 (SEQ ID NO: 318), T46984_PEA_(—)1_T19 (SEQ ID NO: 320), T46984_PEA_(—)1_T23 (SEQ ID NO: 321), T46984_PEA_(—)1_T27 (SEQ ID NO: 322), T46984_PEA_(—)1_T32 (SEQ ID NO: 323), T46984_PEA_(—)1_T34 (SEQ ID NO: 324), T46984_PEA_(—)1_T35 (SEQ ID NO: 325) and T46984_PEA_(—)1_T40 (SEQ ID NO: 326). Table 71 below describes the starting and ending position of this segment on each transcript.

TABLE 71 Segment location on transcripts Segment Segment Transcript name starting position ending position T46984_PEA_1_T2 1330 1407 (SEQ ID NO: 314) T46984_PEA_1_T3 1330 1407 (SEQ ID NO: 315) T46984_PEA_1_T12 1330 1407 (SEQ ID NO: 316) T46984_PEA_1_T13 1330 1407 (SEQ ID NO: 317) T46984_PEA_1_T14 1330 1407 (SEQ ID NO: 318) T46984_PEA_1_T19 1330 1407 (SEQ ID NO: 320) T46984_PEA_1_T23 1330 1407 (SEQ ID NO: 321) T46984_PEA_1_T27 1148 1225 (SEQ ID NO: 322) T46984_PEA_1_T32 1154 1231 (SEQ ID NO: 323) T46984_PEA_1_T34 1330 1407 (SEQ ID NO: 324) T46984_PEA_1_T35 1058 1135 (SEQ ID NO: 325) T46984_PEA_1_T40 1330 1407 (SEQ ID NO: 326)

Segment cluster T46984_PEA_(—)1_node_(—)38 (SEQ ID NO: 359) according to the present invention can be found in the following transcript(s): T46984_PEA_(—)1_T2 (SEQ ID NO: 314), T46984_PEA_(—)1_T3 (SEQ ID NO: 315), T46984_PEA_(—)1_T12 (SEQ ID NO: 316), T46984_PEA_(—)1_T13 (SEQ ID NO: 317), T46984_PEA_(—)1_T14 (SEQ ID NO: 318), T46984_PEA_(—)1_T19 (SEQ ID NO: 320), T46984_PEA_(—)1_T23 (SEQ ID NO: 321), T46984_PEA_(—)1_T27 (SEQ ID NO: 322), T46984_PEA_(—)1_T32 (SEQ ID NO: 323), T46984_PEA_(—)1_T34 (SEQ ID NO: 324) and T46984_PEA_(—)1_T35 (SEQ ID NO: 325). Table 72 below describes the starting and ending position of this segment on each transcript.

TABLE 72 Segment location on transcripts Segment Segment Transcript name starting position ending position T46984_PEA_1_T2 1408 1412 (SEQ ID NO: 314) T46984_PEA_1_T3 1408 1412 (SEQ ID NO: 315) T46984_PEA_1_T12 1408 1412 (SEQ ID NO: 316) T46984_PEA_1_T13 1408 1412 (SEQ ID NO: 317) T46984_PEA_1_T14 1408 1412 (SEQ ID NO: 318) T46984_PEA_1_T19 1408 1412 (SEQ ID NO: 320) T46984_PEA_1_T23 1408 1412 (SEQ ID NO: 321) T46984_PEA_1_T27 1226 1230 (SEQ ID NO: 322) T46984_PEA_1_T32 1232 1236 (SEQ ID NO: 323) T46984_PEA_1_T34 1408 1412 (SEQ ID NO: 324) T46984_PEA_1_T35 1136 1140 (SEQ ID NO: 325)

Segment cluster T46984_PEA_(—)1_node_(—)39 (SEQ ID NO: 360) according to the present invention can be found in the following transcript(s): T46984_PEA_(—)1_T2 (SEQ ID NO: 314), T46984_PEA_(—)1_T3 (SEQ ID NO: 315), T46984_PEA_(—)1_T12 (SEQ ID NO: 316), T46984_PEA_(—)1_T13 (SEQ ID NO: 317), T46984_PEA_(—)1_T14 (SEQ ID NO: 318), T46984_PEA_(—)1_T15 (SEQ ID NO: 319), T46984_PEA_(—)1_T19 (SEQ ID NO: 320), T46984_PEA_(—)1_T23 (SEQ ID NO: 321), T46984_PEA_(—)1_T27 (SEQ ID NO: 322), T46984_PEA_(—)1_T32 (SEQ ID NO: 323), T46984_PEA_(—)1_T34 (SEQ ID NO: 324) and T46984_PEA_(—)1_T35 (SEQ ID NO: 325). Table 73 below describes the starting and ending position of this segment on each transcript.

TABLE 73 Segment location on transcripts Segment Segment Transcript name starting position ending position T46984_PEA_1_T2 1413 1435 (SEQ ID NO: 314) T46984_PEA_1_T3 1413 1435 (SEQ ID NO: 315) T46984_PEA_1_T12 1413 1435 (SEQ ID NO: 316) T46984_PEA_1_T13 1413 1435 (SEQ ID NO: 317) T46984_PEA_1_T14 1413 1435 (SEQ ID NO: 318) T46984_PEA_1_T15 1330 1352 (SEQ ID NO: 319) T46984_PEA_1_T19 1413 1435 (SEQ ID NO: 320) T46984_PEA_1_T23 1413 1435 (SEQ ID NO: 321) T46984_PEA_1_T27 1231 1253 (SEQ ID NO: 322) T46984_PEA_1_T32 1237 1259 (SEQ ID NO: 323) T46984_PEA_1_T34 1413 1435 (SEQ ID NO: 324) T46984_PEA_1_T35 1141 1163 (SEQ ID NO: 325)

Segment cluster T46984_PEA_(—)1_node_(—)40 (SEQ ID NO: 361) according to the present invention is supported by 227 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): T46984_PEA_(—)1_T2 (SEQ ID NO: 314), T46984_PEA_(—)1_T3 (SEQ ID NO: 315), T46984_PEA_(—)1_T12 (SEQ ID NO: 316), T46984_PEA_(—)1_T13 (SEQ ID NO: 317), T46984_PEA_(—)1_T14 (SEQ ID NO: 318), T46984_PEA_(—)1_T15 (SEQ ID NO: 319), T46984_PEA_(—)1_T19 (SEQ ID NO: 320), T46984_PEA_(—)1_T23 (SEQ ID NO: 321), T46984_PEA_(—)1_T27 (SEQ ID NO: 322), T46984_PEA_(—)1_T32 (SEQ ID NO: 323), T46984_PEA_(—)1_T34 (SEQ ID NO: 324) and T46984_PEA_(—)1_T35 (SEQ ID NO: 325). Table 74 below describes the starting and ending position of this segment on each transcript.

TABLE 74 Segment location on transcripts Segment Segment Transcript name starting position ending position T46984_PEA_1_T2 1436 1499 (SEQ ID NO: 314) T46984_PEA_1_T3 1436 1499 (SEQ ID NO: 315) T46984_PEA_1_T12 1436 1499 (SEQ ID NO: 316) T46984_PEA_1_T13 1436 1499 (SEQ ID NO: 317) T46984_PEA_1_T14 1436 1499 (SEQ ID NO: 318) T46984_PEA_1_T15 1353 1416 (SEQ ID NO: 319) T46984_PEA_1_T19 1436 1499 (SEQ ID NO: 320) T46984_PEA_1_T23 1436 1499 (SEQ ID NO: 321) T46984_PEA_1_T27 1254 1317 (SEQ ID NO: 322) T46984_PEA_1_T32 1260 1323 (SEQ ID NO: 323) T46984_PEA_1_T34 1436 1499 (SEQ ID NO: 324) T46984_PEA_1_T35 1164 1227 (SEQ ID NO: 325)

Segment cluster T46984_PEA_(—)1_node_(—)42 (SEQ ID NO: 362) according to the present invention is supported by 239 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): T46984_PEA_(—)1_T2 (SEQ ID NO: 314), T46984_PEA_(—)1_T3 (SEQ ID NO: 315), T46984_PEA_(—)1_T12 (SEQ ID NO: 316), T46984_PEA_(—)1_T13 (SEQ ID NO: 317), T46984_PEA_(—)1_T14 (SEQ ID NO: 318), T46984_PEA_(—)1_T15 (SEQ ID NO: 319), T46984_PEA_(—)1_T19 (SEQ ID NO: 320), T46984_PEA_(—)1_T23 (SEQ ID NO: 321), T46984_PEA_(—)1_T27 (SEQ ID NO: 322), T46984_PEA_(—)1_T32 (SEQ ID NO: 323), T46984_PEA_(—)1_T34 (SEQ ID NO: 324) and T46984_PEA_(—)1_T35 (SEQ ID NO: 325). Table 75 below describes the starting and ending position of this segment on each transcript.

TABLE 75 Segment location on transcripts Segment Segment Transcript name starting position ending position T46984_PEA_1_T2 1500 1562 (SEQ ID NO: 314) T46984_PEA_1_T3 1500 1562 (SEQ ID NO: 315) T46984_PEA_1_T12 1500 1562 (SEQ ID NO: 316) T46984_PEA_1_T13 1500 1562 (SEQ ID NO: 317) T46984_PEA_1_T14 1500 1562 (SEQ ID NO: 318) T46984_PEA_1_T15 1417 1479 (SEQ ID NO: 319) T46984_PEA_1_T19 1500 1562 (SEQ ID NO: 320) T46984_PEA_1_T23 1500 1562 (SEQ ID NO: 321) T46984_PEA_1_T27 1318 1380 (SEQ ID NO: 322) T46984_PEA_1_T32 1324 1386 (SEQ ID NO: 323) T46984_PEA_1_T34 1500 1562 (SEQ ID NO: 324) T46984_PEA_1_T35 1228 1290 (SEQ ID NO: 325)

Segment cluster T46984_PEA_(—)1_node_(—)43 (SEQ ID NO: 363) according to the present invention is supported by 235 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): T46984_PEA_(—)1_T2 (SEQ ID NO: 314), T46984_PEA_(—)1_T3 (SEQ ID NO: 315), T46984_PEA_(—)1_T12 (SEQ ID NO: 316), T46984_PEA_(—)1_T13 (SEQ ID NO: 317), T46984_PEA_(—)1_T14 (SEQ ID NO: 318), T46984_PEA_(—)1_T15 (SEQ ID NO: 319), T46984_PEA_(—)1_T19 (SEQ ID NO: 320), T46984_PEA_(—)1_T23 (SEQ ID NO: 321), T46984_PEA_(—)1_T27 (SEQ ID NO: 322), T46984_PEA_(—)1_T32 (SEQ ID NO: 323) and T46984_PEA_(—)1_T35 (SEQ ID NO: 325). Table 76 below describes the starting and ending position of this segment on each transcript.

TABLE 76 Segment location on transcripts Segment Segment Transcript name starting position ending position T46984_PEA_1_T2 1563 1614 (SEQ ID NO: 314) T46984_PEA_1_T3 1563 1614 (SEQ ID NO: 315) T46984_PEA_1_T12 1563 1614 (SEQ ID NO: 316) T46984_PEA_1_T13 1563 1614 (SEQ ID NO: 317) T46984_PEA_1_T14 1563 1614 (SEQ ID NO: 318) T46984_PEA_1_T15 1480 1531 (SEQ ID NO: 319) T46984_PEA_1_T19 1563 1614 (SEQ ID NO: 320) T46984_PEA_1_T23 1563 1614 (SEQ ID NO: 321) T46984_PEA_1_T27 1381 1432 (SEQ ID NO: 322) T46984_PEA_1_T32 1387 1438 (SEQ ID NO: 323) T46984_PEA_1_T35 1291 1342 (SEQ ID NO: 325)

Segment cluster T46984_PEA_(—)1_node_(—)48 (SEQ ID NO: 364) according to the present invention is supported by 282 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): T46984_PEA_(—)1_T2 (SEQ ID NO: 314), T46984_PEA_(—)1_T3 (SEQ ID NO: 315), T46984_PEA_(—)1_T12 (SEQ ID NO: 316), T46984_PEA_(—)1_T13 (SEQ ID NO: 317), T46984_PEA_(—)1_T14 (SEQ ID NO: 318), T46984_PEA_(—)1_T15 (SEQ ID NO: 319), T46984_PEA_(—)1_T19 (SEQ ID NO: 320), T46984_PEA_(—)1_T23 (SEQ ID NO: 321), T46984_PEA_(—)1_T27 (SEQ ID NO: 322), T46984_PEA_(—)1_T32 (SEQ ID NO: 323), T46984_PEA_(—)1_T35 (SEQ ID NO: 325) and T46984_PEA_(—)1_T46 (SEQ ID NO: 329). Table 77 below describes the starting and ending position of this segment on each transcript.

Table 77 Segment location on transcripts Segment Segment Transcript name starting position ending position T46984_PEA_1_T2 1615 1715 (SEQ ID NO: 314) T46984_PEA_1_T3 2243 2343 (SEQ ID NO: 315) T46984_PEA_1_T12 1615 1715 (SEQ ID NO: 316) T46984_PEA_1_T13 1615 1715 (SEQ ID NO: 317) T46984_PEA_1_T14 1615 1715 (SEQ ID NO: 318) T46984_PEA_1_T15 1532 1632 (SEQ ID NO: 319) T46984_PEA_1_T19 2243 2343 (SEQ ID NO: 320) T46984_PEA_1_T23 1615 1715 (SEQ ID NO: 321) T46984_PEA_1_T27 1433 1533 (SEQ ID NO: 322) T46984_PEA_1_T32 1439 1539 (SEQ ID NO: 323) T46984_PEA_1_T35 1343 1443 (SEQ ID NO: 325) T46984_PEA_1_T46 935 1035 (SEQ ID NO: 329)

Segment cluster T46984_PEA_(—)1_node_(—)49 (SEQ ID NO: 365) according to the present invention is supported by 262 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): T46984_PEA_(—)1_T2 (SEQ ID NO: 314), T46984_PEA_(—)1_T3 (SEQ ID NO: 315), T46984_PEA_(—)1_T12 (SEQ ID NO: 316), T46984_PEA_(—)1_T13 (SEQ ID NO: 317), T46984_PEA_(—)1_T14 (SEQ ID NO: 318), T46984_PEA_(—)1_T15 (SEQ ID NO: 319), T46984_PEA_(—)1_T19 (SEQ ID NO: 320), T46984_PEA_(—)1_T23 (SEQ ID NO: 321), T46984_PEA_(—)1_T27 (SEQ ID NO: 322), T46984_PEA_(—)1_T32 (SEQ ID NO: 323), T46984_PEA_(—)1_T35 (SEQ ID NO: 325) and T46984_PEA_(—)1_T46 (SEQ ID NO: 329). Table 78 below describes the starting and ending position of this segment on each transcript.

TABLE 78 Segment location on transcripts Segment Segment Transcript name starting position ending position T46984_PEA_1_T2 1716 1757 (SEQ ID NO: 314) T46984_PEA_1_T3 2344 2385 (SEQ ID NO: 315) T46984_PEA_1_T12 1716 1757 (SEQ ID NO: 316) T46984_PEA_1_T13 1716 1757 (SEQ ID NO: 317) T46984_PEA_1_T14 1716 1757 (SEQ ID NO: 318) T46984_PEA_1_T15 1633 1674 (SEQ ID NO: 319) T46984_PEA_1_T19 2344 2385 (SEQ ID NO: 320) T46984_PEA_1_T23 1716 1757 (SEQ ID NO: 321) T46984_PEA_1_T27 1534 1575 (SEQ ID NO: 322) T46984_PEA_1_T32 1540 1581 (SEQ ID NO: 323) T46984_PEA_1_T35 1444 1485 (SEQ ID NO: 325) T46984_PEA_1_T46 1036 1077 (SEQ ID NO: 329)

Segment cluster T46984_PEA_(—)1_node_(—)50 (SEQ ID NO: 366) according to the present invention is supported by 277 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): T46984_PEA_(—)1_T2 (SEQ ID NO: 314), T46984_PEA_(—)1_T3 (SEQ ID NO: 315), T46984_PEA_(—)1_T12 (SEQ ID NO: 316), T46984_PEA_(—)1_T13 (SEQ ID NO: 317), T46984_PEA_(—)1_T14 (SEQ ID NO: 318), T46984_PEA_(—)1_T15 (SEQ ID NO: 319), T46984_PEA_(—)1_T19 (SEQ ID NO: 320), T46984_PEA_(—)1_T23 (SEQ ID NO: 321), T46984_PEA_(—)1_T27 (SEQ ID NO: 322), T46984_PEA_(—)1_T32 (SEQ ID NO: 323), T46984_PEA_(—)1_T35 (SEQ ID NO: 325) and T46984_PEA_(—)1_T46 (SEQ ID NO: 329). Table 79 below describes the starting and ending position of this segment on each transcript.

TABLE 79 Segment location on transcripts Segment Segment Transcript name starting position ending position T46984_PEA_1_T2 1758 1809 (SEQ ID NO: 314) T46984_PEA_1_T3 2386 2437 (SEQ ID NO: 315) T46984_PEA_1_T12 1758 1809 (SEQ ID NO: 316) T46984_PEA_1_T13 1758 1809 (SEQ ID NO: 317) T46984_PEA_1_T14 1758 1809 (SEQ ID NO: 318) T46984_PEA_1_T15 1675 1726 (SEQ ID NO: 319) T46984_PEA_1_T19 2386 2437 (SEQ ID NO: 320) T46984_PEA_1_T23 1758 1809 (SEQ ID NO: 321) T46984_PEA_1_T27 1576 1627 (SEQ ID NO: 322) T46984_PEA_1_T32 1582 1633 (SEQ ID NO: 323) T46984_PEA_1_T35 1486 1537 (SEQ ID NO: 325) T46984_PEA_1_T46 1078 1129 (SEQ ID NO: 329)

Segment cluster T46984_PEA_(—)1_node_(—)51 (SEQ ID NO: 367) according to the present invention is supported by 6 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): T46984_PEA_(—)1_T2 (SEQ ID NO: 314), T46984_PEA_(—)1_T12 (SEQ ID NO: 316), T46984_PEA_(—)1_T19 (SEQ ID NO: 320) and T46984_PEA_(—)1_T23 (SEQ ID NO: 321). Table 80 below describes the starting and ending position of this segment on each transcript.

TABLE 80 Segment location on transcripts Segment Segment Transcript name starting position ending position T46984_PEA_1_T2 1810 1837 (SEQ ID NO: 314) T46984_PEA_1_T12 1810 1837 (SEQ ID NO: 316) T46984_PEA_1_T19 2438 2465 (SEQ ID NO: 320) T46984_PEA_1_T23 1810 1837 (SEQ ID NO: 321)

Segment cluster T46984_PEA_(—)1_node_(—)53 (SEQ ID NO: 368) according to the present invention is supported by 16 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): T46984_PEA_(—)1_T2 (SEQ ID NO: 314), T46984_PEA_(—)1_T13 (SEQ ID NO: 317), T46984_PEA_(—)1_T19 (SEQ ID NO: 320) and T46984_PEA_(—)1_T23 (SEQ ID NO: 321). Table 81 below describes the starting and ending position of this segment on each transcript.

TABLE 81 Segment location on transcripts Segment Segment Transcript name starting position ending position T46984_PEA_1_T2 2905 2963 (SEQ ID NO: 314) T46984_PEA_1_T13 1810 1868 (SEQ ID NO: 317) T46984_PEA_1_T19 3533 3591 (SEQ ID NO: 320) T46984_PEA_1_T23 2905 2963 (SEQ ID NO: 321)

Segment cluster T46984_PEA_(—)1_node_(—)54 (SEQ ID NO: 369) according to the present invention is supported by 18 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): T46984_PEA_(—)1_T2 (SEQ ID NO: 314), T46984_PEA_(—)1_T19 (SEQ ID NO: 320) and T46984_PEA_(—)1_T23 (SEQ ID NO: 321). Table 82 below describes the starting and ending position of this segment on each transcript.

TABLE 82 Segment location on transcripts Segment Segment Transcript name starting position ending position T46984_PEA_1_T2 2964 3043 T46984_PEA_1_T19 3592 3671 (SEQ ID NO: 320) T46984_PEA_1_T23 2964 3043 (SEQ ID NO: 321)

Segment cluster T46984_PEA_(—)1_node_(—)55 (SEQ ID NO: 370) according to the present invention is supported by 335 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): T46984_PEA_(—)1_T2, T46984_PEA_(—)1_T3 (SEQ ID NO: 315), T46984_PEA_(—)1_T12 (SEQ ID NO: 316), T46984_PEA_(—)1_T13 (SEQ ID NO: 317), T46984_PEA_(—)1_T14 (SEQ ID NO: 318), T46984_PEA_(—)1_T15 (SEQ ID NO: 319), T46984_PEA_(—)1_T19 (SEQ ID NO: 320), T46984_PEA_(—)1_T23 (SEQ ID NO: 321), T46984_PEA_(—)1_T27 (SEQ ID NO: 322), T46984_PEA_(—)1_T32 (SEQ ID NO: 323), T46984_PEA_(—)1_T35 (SEQ ID NO: 325) and T46984_PEA_(—)1_T46 (SEQ ID NO: 329). Table 83 below describes the starting and ending position of this segment on each transcript.

TABLE 83 Segment location on transcripts Segment Segment Transcript name starting position ending position T46984_PEA_1_T2 3044 3110 T46984_PEA_1_T3 2438 2504 (SEQ ID NO: 315) T46984_PEA_1_T12 1838 1904 (SEQ ID NO: 316) T46984_PEA_1_T13 1869 1935 (SEQ ID NO: 317) T46984_PEA_1_T14 1810 1876 (SEQ ID NO: 318) T46984_PEA_1_T15 1727 1793 (SEQ ID NO: 319) T46984_PEA_1_T19 3672 3738 (SEQ ID NO: 320) T46984_PEA_1_T23 3044 3110 (SEQ ID NO: 321) T46984_PEA_1_T27 1628 1694 (SEQ ID NO: 322) T46984_PEA_1_T32 1634 1700 (SEQ ID NO: 323) T46984_PEA_1_T35 1538 1604 (SEQ ID NO: 325) T46984_PEA_1_T46 1130 1196 (SEQ ID NO: 329)

Segment cluster T46984_PEA_(—)1_node_(—)57 (SEQ ID NO: 371) according to the present invention can be found in the following transcript(s): T46984_PEA_(—)1_T2, T46984_PEA_(—)1_T3 (SEQ ID NO: 315), T46984_PEA_(—)1_T12 (SEQ ID NO: 316), T46984_PEA_(—)1_T13 (SEQ ID NO: 317), T46984_PEA_(—)1_T14 (SEQ ID NO: 318), T46984_PEA_(—)1_T15 (SEQ ID NO: 319), T46984_PEA_(—)1_T19 (SEQ ID NO: 320), T46984_PEA_(—)1_T23 (SEQ ID NO: 321), T46984_PEA_(—)1_T27 (SEQ ID NO: 322), T46984_PEA_(—)1_T32 (SEQ ID NO: 323), T46984_PEA_(—)1_T35 (SEQ ID NO: 325) and T46984_PEA_(—)1_T46 (SEQ ID NO: 329). Table 84 below describes the starting and ending position of this segment on each transcript.

TABLE 84 Segment location on transcripts Segment Segment Transcript name starting position ending position T46984_PEA_1_T2 3111 3130 T46984_PEA_1_T3 2505 2524 (SEQ ID NO: 315) T46984_PEA_1_T12 1905 1924 (SEQ ID NO: 316) T46984_PEA_1_T13 1936 1955 (SEQ ID NO: 317) T46984_PEA_1_T14 1877 1896 (SEQ ID NO: 318) T46984_PEA_1_T15 1794 1813 (SEQ ID NO: 319) T46984_PEA_1_T19 3739 3758 (SEQ ID NO: 320) T46984_PEA_1_T23 3111 3130 (SEQ ID NO: 321) T46984_PEA_1_T27 1695 1714 (SEQ ID NO: 322) T46984_PEA_1_T32 1701 1720 (SEQ ID NO: 323) T46984_PEA_1_T35 1605 1624 (SEQ ID NO: 325) T46984_PEA_1_T46 1197 1216 (SEQ ID NO: 329)

Segment cluster T46984_PEA_(—)1_node_(—)6 (SEQ ID NO: 337)0 according to the present invention is supported by 326 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): T46984_PEA_(—)1_T2, T46984_PEA_(—)1_T3 (SEQ ID NO: 315), T46984_PEA_(—)1_T12 (SEQ ID NO: 316), T46984_PEA_(—)1_T13 (SEQ ID NO: 317), T46984_PEA_(—)1_T14 (SEQ ID NO: 318), T46984_PEA_(—)1_T15 (SEQ ID NO: 319), T46984_PEA_(—)1_T19 (SEQ ID NO: 320), T46984_PEA_(—)1_T27 (SEQ ID NO: 322), T46984_PEA_(—)1_T32 (SEQ ID NO: 323), T46984_PEA_(—)1_T35 (SEQ ID NO: 325) and T46984_PEA_(—)1_T46 (SEQ ID NO: 329). Table 85 below describes the starting and ending position of this segment on each transcript.

TABLE 85 Segment location on transcripts Segment Segment Transcript name starting position ending position T46984_PEA_1_T2 3131 3165 T46984_PEA_1_T3 2525 2559 (SEQ ID NO: 315) T46984_PEA_1_T12 1925 1959 (SEQ ID NO: 316) T46984_PEA_1_T13 1956 1990 (SEQ ID NO: 317) T46984_PEA_1_T14 1897 1931 (SEQ ID NO: 318) T46984_PEA_1_T15 1814 1848 (SEQ ID NO: 319) T46984_PEA_1_T19 3759 3793 (SEQ ID NO: 320) T46984_PEA_1_T27 1715 1749 (SEQ ID NO: 322) T46984_PEA_1_T32 1721 1755 (SEQ ID NO: 323) T46984_PEA_1_T35 1625 1659 (SEQ ID NO: 325) T46984_PEA_1_T46 1217 1251 (SEQ ID NO: 329)

Segment cluster T46984_PEA_(—)1_node_(—)62 (SEQ ID NO: 373) according to the present invention is supported by 335 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): T46984_PEA_(—)1_T2, T46984_PEA_(—)1_T3 (SEQ ID NO: 315), T46984_PEA_(—)1_T12 (SEQ ID NO: 316), T46984_PEA_(—)1_T13 (SEQ ID NO:317), T46984_PEA_(—)1_T14 (SEQ ID NO:318), T46984_PEA_(—)1_T15 (SEQ ID NO: 319), T46984_PEA_(—)1_T19 (SEQ ID NO: 320), T46984_PEA_(—)1_T27 (SEQ ID NO: 322), T46984_PEA_(—)1_T32 (SEQ ID NO: 323), T46984_PEA_(—)1_T35 (SEQ ID NO: 325) and T46984_PEA_(—)1_T46 (SEQ ID NO: 329). Table 86 below describes the starting and ending position of this segment on each transcript.

TABLE 86 Segment location on transcripts Segment Segment Transcript name starting position ending position T46984_PEA_1_T2 3166 3226 T46984_PEA_1_T3 2560 2620 (SEQ ID NO: 315) T46984_PEA_1_T12 1960 2020 (SEQ ID NO: 316) T46984_PEA_1_T13 1991 2051 (SEQ ID NO: 317) T46984_PEA_1_T14 1932 1992 (SEQ ID NO: 318) T46984_PEA_1_T15 1849 1909 (SEQ ID NO: 319) T46984_PEA_1_T19 3794 3854 (SEQ ID NO: 320) T46984_PEA_1_T27 1750 1810 (SEQ ID NO: 322) T46984_PEA_1_T32 1756 1816 (SEQ ID NO: 323) T46984_PEA_1_T35 1660 1720 (SEQ ID NO: 325) T46984_PEA_1_T46 1252 1312 (SEQ ID NO: 329)

Segment cluster T46984_PEA_(—)1_node_(—)6 (SEQ ID NO: 337)6 according to the present invention is supported by 336 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): T46984_PEA_(—)1_T2, T46984_PEA_(—)1_T3 (SEQ ID NO: 315), T46984_PEA_(—)1_T12 (SEQ ID NO: 316), T46984_PEA_(—)1_T13 (SEQ ID NO: 317), T46984_PEA_(—)1_T14 (SEQ ID NO: 318), T46984_PEA_(—)1_T15 (SEQ ID NO: 319), T46984_PEA_(—)1_T19 (SEQ ID NO: 320), T46984_PEA_(—)1_T23 (SEQ ID NO: 321), T46984_PEA_(—)1_T27 (SEQ ID NO: 322), T46984_PEA_(—)1_T32 (SEQ ID NO: 323), T46984_PEA_(—)1_T34 (SEQ ID NO: 324), T46984_PEA_(—)1_T35 (SEQ ID NO: 325), T46984_PEA_(—)1_T46 (SEQ ID NO: 329), T46984_PEA_(—)1_T47 (SEQ ID NO: 330) and T46984_PEA_(—)1_T51 (SEQ ID NO: 332). Table 87 below describes the starting and ending position of this segment on each transcript.

TABLE 87 Segment location on transcripts Segment Segment Transcript name starting position ending position T46984_PEA_1_T2 3227 3261 T46984_PEA_1_T3 2621 2655 (SEQ ID NO: 315) T46984_PEA_1_T12 2021 2055 (SEQ ID NO: 316) T46984_PEA_1_T13 2052 2086 (SEQ ID NO: 317) T46984_PEA_1_T14 1993 2027 (SEQ ID NO: 318) T46984_PEA_1_T15 1910 1944 (SEQ ID NO: 319) T46984_PEA_1_T19 3855 3889 (SEQ ID NO: 320) T46984_PEA_1_T23 3131 3165 (SEQ ID NO: 321) T46984_PEA_1_T27 1811 1845 (SEQ ID NO: 322) T46984_PEA_1_T32 1817 1851 (SEQ ID NO: 323) T46984_PEA_1_T34 1563 1597 (SEQ ID NO: 324) T46984_PEA_1_T35 1721 1755 (SEQ ID NO: 325) T46984_PEA_1_T46 1313 1347 (SEQ ID NO: 329) T46984_PEA_1_T47 752 786 (SEQ ID NO: 330) T46984_PEA_1_T51 349 383 (SEQ ID NO: 332)

Segment cluster T46984_PEA_(—)1_node_(—)6 (SEQ ID NO: 337)7 according to the present invention is supported by 323 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): T46984_PEA_(—)1_T2, T46984_PEA_(—)1_T3 (SEQ ID NO: 315), T46984_PEA_(—)1_T12 (SEQ ID NO: 316), T46984_PEA_(—)1_T13 (SEQ ID NO: 317), T46984_PEA_(—)1_T14 (SEQ ID NO: 318), T46984_PEA_(—)1_T15 (SEQ ID NO: 319), T46984_PEA_(—)1_T19 (SEQ ID NO: 320), T46984_PEA_(—)1_T23 (SEQ ID NO: 321), T46984_PEA_(—)1_T27 (SEQ ID NO: 322), T46984_PEA_(—)1_T32 (SEQ ID NO: 323), T46984_PEA_(—)1_T34 (SEQ ID NO: 324), T46984_PEA_(—)1_T35 (SEQ ID NO: 325), T46984_PEA_(—)1_T46 (SEQ ID NO: 329), T46984_PEA_(—)1_T47 (SEQ ID NO: 330) and T46984_PEA_(—)1_T51 (SEQ ID NO: 332). Table 88 below describes the starting and ending position of this segment on each transcript.

TABLE 88 Segment location on transcripts Segment Segment Transcript name starting position ending position T46984_PEA_1_T2 3262 3302 T46984_PEA_1_T3 2656 2696 (SEQ ID NO: 315) T46984_PEA_1_T12 2056 2096 (SEQ ID NO: 316) T46984_PEA_1_T13 2087 2127 (SEQ ID NO: 317) T46984_PEA_1_T14 2028 2068 (SEQ ID NO: 318) T46984_PEA_1_T15 1945 1985 (SEQ ID NO: 319) T46984_PEA_1_T19 3890 3930 (SEQ ID NO: 320) T46984_PEA_1_T23 3166 3206 (SEQ ID NO: 321) T46984_PEA_1_T27 1846 1886 (SEQ ID NO: 322) T46984_PEA_1_T32 1852 1892 (SEQ ID NO: 323) T46984_PEA_1_T34 1598 1638 (SEQ ID NO: 324) T46984_PEA_1_T35 1756 1796 (SEQ ID NO: 325) T46984_PEA_1_T46 1348 1388 (SEQ ID NO: 329) T46984_PEA_1_T47 787 827 (SEQ ID NO: 330) T46984_PEA_1_T51 384 424 (SEQ ID NO: 332)

Segment cluster T46984_PEA_(—)1_node_(—)70 (SEQ ID NO: 376) according to the present invention is supported by 337 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): T46984_PEA_(—)1_T2, T46984_PEA_(—)1_T3 (SEQ ID NO: 315), T46984_PEA_(—)1_T12 (SEQ ID NO: 316), T46984_PEA_(—)1_T13 (SEQ ID NO: 317), T46984_PEA_(—)1_T14 (SEQ ID NO: 318), T46984_PEA_(—)1_T15 (SEQ ID NO: 319), T46984_PEA_(—)1_T19 (SEQ ID NO: 320), T46984_PEA_(—)1_T23 (SEQ ID NO: 321), T46984_PEA_(—)1_T27 (SEQ ID NO: 322), T46984_PEA_(—)1_T32 (SEQ ID NO: 323), T46984_PEA_(—)1_T34 (SEQ ID NO: 324), T46984_PEA_(—)1_T35 (SEQ ID NO: 325), T46984_PEA_(—)1_T46 (SEQ ID NO: 329), T46984_PEA_(—)1_T47 (SEQ ID NO: 330), T46984_PEA_(—)1_T51 (SEQ ID NO: 332), T46984_PEA_(—)1_T52 (SEQ ID NO: 333) and T46984_PEA_(—)1_T54 (SEQ ID NO: 334). Table 89 below describes the starting and ending position of this segment on each transcript.

TABLE 89 Segment location on transcripts Segment Segment Transcript name starting position ending position T46984_PEA_1_T2 3303 3377 T46984_PEA_1_T3 2697 2771 (SEQ ID NO: 315) T46984_PEA_1_T12 2097 2171 (SEQ ID NO: 316) T46984_PEA_1_T13 2128 2202 (SEQ ID NO: 317) T46984_PEA_1_T14 2069 2143 (SEQ ID NO: 318) T46984_PEA_1_T15 1986 2060 (SEQ ID NO: 319) T46984_PEA_1_T19 3931 4005 (SEQ ID NO: 320) T46984_PEA_1_T23 3207 3281 (SEQ ID NO: 321) T46984_PEA_1_T27 1887 1961 (SEQ ID NO: 322) T46984_PEA_1_T32 1893 1967 (SEQ ID NO: 323) T46984_PEA_1_T34 1639 1713 (SEQ ID NO: 324) T46984_PEA_1_T35 1797 1871 (SEQ ID NO: 325) T46984_PEA_1_T46 1389 1463 (SEQ ID NO: 329) T46984_PEA_1_T47 828 902 (SEQ ID NO: 330) T46984_PEA_1_T51 425 499 (SEQ ID NO: 332) T46984_PEA_1_T52 928 1002 (SEQ ID NO: 333) T46984_PEA_1_T54 928 1002 (SEQ ID NO: 334)

Segment cluster T46984_PEA_(—)1_node_(—)71 (SEQ ID NO: 377) according to the present invention can be found in the following transcript(s): T46984_PEA_(—)1_T2, T46984_PEA_(—)1_T3 (SEQ ID NO: 315), T46984_PEA_(—)1_T12 (SEQ ID NO: 316), T46984_PEA_(—)1_T13 (SEQ ID NO: 317), T46984_PEA_(—)1_T14 (SEQ ID NO: 318), T46984_PEA_(—)1_T15 (SEQ ID NO: 319), T46984_PEA_(—)1_T19 (SEQ ID NO: 320), T46984_PEA_(—)1_T23 (SEQ ID NO: 321), T46984_PEA_(—)1_T27 (SEQ ID NO: 322), T46984_PEA_(—)1_T32 (SEQ ID NO: 323), T46984_PEA_(—)1_T34 (SEQ ID NO: 324), T46984_PEA_(—)1_T35 (SEQ ID NO: 325), T46984_PEA_(—)1_T46 (SEQ ID NO: 329), T46984_PEA_(—)1_T47 (SEQ ID NO: 330), T46984_PEA_(—)1_T51 (SEQ ID NO: 332), T46984_PEA_(—)1_T52 (SEQ ID NO: 333) and T46984_PEA_(—)1_T54 (SEQ ID NO: 334). Table 90 below describes the starting and ending position of this segment on each transcript.

TABLE 90 Segment location on transcripts Segment Segment Transcript name starting position ending position T46984_PEA_1_T2 3378 3399 T46984_PEA_1_T3 2772 2793 (SEQ ID NO: 315) T46984_PEA_1_T12 2172 2193 (SEQ ID NO: 316) T46984_PEA_1_T13 2203 2224 (SEQ ID NO: 317) T46984_PEA_1_T14 2144 2165 (SEQ ID NO: 318) T46984_PEA_1_T15 2061 2082 (SEQ ID NO: 319) T46984_PEA_1_T19 4006 4027 (SEQ ID NO: 320) 146984_PEA_1_T23 3282 3303 (SEQ ID NO: 321) 146984_PEA_1_T27 1962 1983 (SEQ ID NO: 322) T46984_PEA_1_T32 1968 1989 (SEQ ID NO: 323) T46984_PEA_1_T34 1714 1735 (SEQ ID NO: 324) T46984_PEA_1_T35 1872 1893 (SEQ ID NO: 325) 146984_PEA_1_T46 1464 1485 (SEQ ID NO: 329) 146984_PEA_1_T47 903 924 (SEQ ID NO: 330) T46984_PEA_1_T51 500 521 (SEQ ID NO: 332) T46984_PEA_1_T52 1003 1024 (SEQ ID NO: 333) T46984_PEA_1_T54 1003 1024 (SEQ ID NO: 334)

Segment cluster T46984_PEA_(—)1_node_(—)72 (SEQ ID NO: 378) according to the present invention can be found in the following transcript(s): T46984_PEA_(—)1_T2, T46984_PEA_(—)1_T3 (SEQ ID NO: 315), T46984_PEA_(—)1_T12 (SEQ ID NO: 316), T46984_PEA_(—)1_T13 (SEQ ID NO: 317), T46984_PEA_(—)1_T14 (SEQ ID NO: 318), T46984_PEA_(—)1_T15 (SEQ ID NO: 319), T46984_PEA_(—)1_T19 (SEQ ID NO: 320), T46984_PEA_(—)1_T23 (SEQ ID NO: 321), T46984_PEA_(—)1_T27 (SEQ ID NO: 322), T46984_PEA_(—)1_T32 (SEQ ID NO: 323), T46984_PEA_(—)1_T34 (SEQ ID NO: 324), T46984_PEA_(—)1_T35 (SEQ ID NO: 325), T46984_PEA_(—)1_T43 (SEQ ID NO: 328), T46984_PEA_(—)1_T46 (SEQ ID NO: 329), T46984_PEA_(—)1_T47 (SEQ ID NO: 330), T46984_PEA_(—)1_T51 (SEQ ID NO: 332), T46984_PEA_(—)1_T52 (SEQ ID NO: 333) and T46984_PEA_(—)1_T54 (SEQ ID NO: 334). Table 91 below describes the starting and ending position of this segment on each transcript.

TABLE 91 Segment location on transcripts Segment Segment Transcript name starting position ending position T46984_PEA_1_T2 3400 3421 T46984_PEA_1_T3 2794 2815 (SEQ ID NO: 315) T46984_PEA_1_T12 2194 2215 (SEQ ID NO: 316) T46984_PEA_1_T13 2225 2246 (SEQ ID NO: 317) T46984_PEA_1_T14 2166 2187 (SEQ ID NO: 318) T46984_PEA_1_T15 2083 2104 (SEQ ID NO: 319) T46984_PEA_1_T19 4028 4049 (SEQ ID NO: 320) T46984_PEA_1_T23 3304 3325 (SEQ ID NO: 321) T46984_PEA_1_T27 1984 2005 (SEQ ID NO: 322) T46984_PEA_1_T32 1990 2011 (SEQ ID NO: 323) T46984_PEA_1_T34 1736 1757 (SEQ ID NO: 324) T46984_PEA_1_T35 1894 1915 (SEQ ID NO: 325) T46984_PEA_1_T43 1172 1193 (SEQ ID NO: 328) T46984_PEA_1_T46 1486 1507 (SEQ ID NO: 329) T46984_PEA_1_T47 925 946 (SEQ ID NO: 330) T46984_PEA_1_T51 522 543 (SEQ ID NO: 332) T46984_PEA_1_T52 1025 1046 (SEQ ID NO: 333) T46984_PEA_1_T54 1025 1046 (SEQ ID NO: 334)

Segment cluster T46984_PEA_(—)1_node_(—)73 (SEQ ID NO: 379) according to the present invention can be found in the following transcript(s): T46984_PEA_(—)1_T2, T46984_PEA_(—)1_T3 (SEQ ID NO: 315), T46984_PEA_(—)1_T12 (SEQ ID NO: 316), T46984_PEA_(—)1_T13 (SEQ ID NO: 317), T46984_PEA_(—)1_T14 (SEQ ID NO: 318), T46984_PEA_(—)1_T15 (SEQ ID NO: 319), T46984_PEA_(—)1_T19 (SEQ ID NO: 320), T46984_PEA_(—)1_T23 (SEQ ID NO: 321), T46984_PEA_(—)1_T27 (SEQ ID NO: 322), T46984_PEA_(—)1_T32 (SEQ ID NO: 323), T46984_PEA_(—)1_T34 (SEQ ID NO: 324), T46984_PEA_(—)1_T35 (SEQ ID NO: 325), T46984_PEA_(—)1_T43 (SEQ ID NO: 328), T46984_PEA_(—)1_T46 (SEQ ID NO: 329), T46984_PEA_(—)1_T47 (SEQ ID NO: 330), T46984_PEA_(—)1_T51 (SEQ ID NO: 332), T46984_PEA_(—)1_T52 (SEQ ID NO: 333) and T46984_PEA_(—)1_T54 (SEQ ID NO: 334). Table 92 below describes the starting and ending position of this segment on each transcript.

TABLE 92 Segment location on transcripts Segment Segment Transcript name starting position ending position T46984_PEA_1_T2 3422 3428 T46984_PEA_1_T3 2816 2822 (SEQ ID NO: 315) T46984_PEA_1_T12 2216 2222 (SEQ ID NO: 316) T46984_PEA_1_T13 2247 2253 (SEQ ID NO: 317) T46984_PEA_1_T14 2188 2194 (SEQ ID NO: 318) T46984_PEA_1_T15 2105 2111 (SEQ ID NO: 319) T46984_PEA_1_T19 4050 4056 (SEQ ID NO: 320) T46984_PEA_1_T23 3326 3332 (SEQ ID NO: 321) T46984_PEA_1_T27 2006 2012 (SEQ ID NO: 322) T46984_PEA_1_T32 2012 2018 (SEQ ID NO: 323) T46984_PEA_1_T34 1758 1764 (SEQ ID NO: 324) T46984_PEA_1_T35 1916 1922 (SEQ ID NO: 325) T46984_PEA_1_T43 1194 1200 (SEQ ID NO: 328) T46984_PEA_1_T46 1508 1514 (SEQ ID NO: 329) T46984_PEA_1_T47 947 953 (SEQ ID NO: 330) T46984_PEA_1_T51 544 550 (SEQ ID NO: 332) T46984_PEA_1_T52 1047 1053 (SEQ ID NO: 333) T46984_PEA_1_T54 1047 1053 (SEQ ID NO: 334)

Segment cluster T46984_PEA_(—)1_node_(—)74 (SEQ ID NO: 380) according to the present invention can be found in the following transcript(s): T46984_PEA_(—)1_T2, T46984_PEA_(—)1_T3 (SEQ ID NO: 315), T46984_PEA_(—)1_T12 (SEQ ID NO: 316), T46984_PEA_(—)1_T13 (SEQ ID NO: 317), T46984_PEA_(—)1_T14 (SEQ ID NO: 318), T46984_PEA_(—)1_T15 (SEQ ID NO: 319), T46984_PEA_(—)1_T19 (SEQ ID NO: 320), T46984_PEA_(—)1_T23 (SEQ ID NO: 321), T46984_PEA_(—)1_T27 (SEQ ID NO: 322), T46984_PEA_(—)1_T32 (SEQ ID NO: 323), T46984_PEA_(—)1_T34 (SEQ ID NO: 324), T46984_PEA_(—)1_T35 (SEQ ID NO: 325), T46984_PEA_(—)1_T43 (SEQ ID NO: 328), T46984_PEA_(—)1_T46 (SEQ ID NO: 329), T46984_PEA_(—)1_T47 (SEQ ID NO: 330), T46984_PEA_(—)1_T51 (SEQ ID NO: 332), T46984_PEA_(—)1_T52 (SEQ ID NO: 333) and T46984_PEA_(—)1_T54 (SEQ ID NO: 334). Table 93 below describes the starting and ending position of this segment on each transcript.

TABLE 93 Segment location on transcripts Segment Segment Transcript name starting position ending position T46984_PEA_1_T2 3429 3432 T46984_PEA_1_T3 2823 2826 (SEQ ID NO: 315) T46984_PEA_1_T12 2223 2226 (SEQ ID NO: 316) T46984_PEA_1_T13 2254 2257 (SEQ ID NO: 317) T46984_PEA_1_T14 2195 2198 (SEQ ID NO: 318) T46984_PEA_1_T15 2112 2115 (SEQ ID NO: 319) T46984_PEA_1_T19 4057 4060 (SEQ ID NO: 320) T46984_PEA_1_T23 3333 3336 (SEQ ID NO: 321) T46984_PEA_1_T27 2013 2016 (SEQ ID NO: 322) T46984_PEA_1_T32 2019 2022 (SEQ ID NO: 323) T46984_PEA_1_T34 1765 1768 (SEQ ID NO: 324) T46984_PEA_1_T35 1923 1926 (SEQ ID NO: 325) T46984_PEA_1_T43 1201 1204 (SEQ ID NO: 328) T46984_PEA_1_T46 1515 1518 (SEQ ID NO: 329) T46984_PEA_1_T47 954 957 (SEQ ID NO: 330) T46984_PEA_1_T51 551 554 (SEQ ID NO: 332) T46984_PEA_1_T52 1054 1057 (SEQ ID NO: 333) T46984_PEA_1_T54 1054 1057 (SEQ ID NO: 334)

Segment cluster T46984_PEA_(—)1_node_(—)83 (SEQ ID NO: 381) according to the present invention can be found in the following transcript(s): T46984_PEA_(—)1_T2, T46984_PEA_(—)1_T3 (SEQ ID NO: 315), T46984_PEA_(—)1_T12 (SEQ ID NO: 316), T46984_PEA_(—)1_T13 (SEQ ID NO: 317), T46984_PEA_(—)1_T15 (SEQ ID NO: 319), T46984_PEA_(—)1_T19 (SEQ ID NO: 320), T46984_PEA_(—)1_T23 (SEQ ID NO: 321), T46984_PEA_(—)1_T27 (SEQ ID NO: 322), T46984_PEA_(—)1_T32 (SEQ ID NO: 323), T46984_PEA_(—)1_T34 (SEQ ID NO: 324), T46984_PEA_(—)1_T35 (SEQ ID NO: 325), T46984_PEA_(—)1_T43 (SEQ ID NO: 328), T46984_PEA_(—)1_T46 (SEQ ID NO: 329), T46984_PEA_(—)1_T47 (SEQ ID NO: 330), T46984_PEA_(—)1_T51 (SEQ ID NO: 332), T46984_PEA_(—)1_T52 (SEQ ID NO: 333) and T46984_PEA_(—)1_T54 (SEQ ID NO: 334). Table 94 below describes the starting and ending position of this segment on each transcript.

TABLE 94 Segment location on transcripts Segment Segment Transcript name starting position ending position T46984_PEA_1_T2 3433 3437 T46984_PEA_1_T3 2827 2831 (SEQ ID NO: 315) T46984_PEA_1_T12 2227 2231 (SEQ ID NO: 316) T46984_PEA_1_T13 2258 2262 (SEQ ID NO: 317) T46984_PEA_1_T15 2116 2120 (SEQ ID NO: 319) T46984_PEA_1_T19 4061 4065 (SEQ ID NO: 320) T46984_PEA_1_T23 3337 3341 (SEQ ID NO: 321) T46984_PEA_1_T27 2017 2021 (SEQ ID NO: 322) T46984_PEA_1_T32 2023 2027 (SEQ ID NO: 323) T46984_PEA_1_T34 1769 1773 (SEQ ID NO: 324) T46984_PEA_1_T35 1927 1931 (SEQ ID NO: 325) T46984_PEA_1_T43 1205 1209 (SEQ ID NO: 328) T46984_PEA_1_T46 1519 1523 (SEQ ID NO: 329) T46984_PEA_1_T47 958 962 (SEQ ID NO: 330) T46984_PEA_1_T51 555 559 (SEQ ID NO: 332) T46984_PEA_1_T52 1058 1062 (SEQ ID NO: 333) T46984_PEA_1_T54 1058 1062 (SEQ ID NO: 334)

Segment cluster T46984_PEA_(—)1_node_(—)84 (SEQ ID NO: 382) according to the present invention can be found in the following transcript(s): T46984_PEA_(—)1_T2, T46984_PEA_(—)1_T3 (SEQ ID NO: 315), T46984_PEA_(—)1_T12 (SEQ ID NO: 316), T46984_PEA_(—)1_T13 (SEQ ID NO: 317), T46984_PEA_(—)1_T15 (SEQ ID NO: 319), T46984_PEA_(—)1_T19 (SEQ ID NO: 320), T46984_PEA_(—)1_T23 (SEQ ID NO: 321), T46984_PEA_(—)1_T27 (SEQ ID NO: 322), T46984_PEA_(—)1_T32 (SEQ ID NO: 323), T46984_PEA_(—)1_T34 (SEQ ID NO: 324), T46984_PEA_(—)1_T35 (SEQ ID NO: 325), T46984_PEA_(—)1_T43 (SEQ ID NO: 328), T46984_PEA_(—)1_T46 (SEQ ID NO: 329), T46984_PEA_(—)1_T47 (SEQ ID NO: 330), T46984_PEA_(—)1_T51 (SEQ ID NO: 332), T46984_PEA_(—)1_T52 (SEQ ID NO: 333) and T46984_PEA_(—)1_T54 (SEQ ID NO: 334). Table 95 below describes the starting and ending position of this segment on each transcript.

TABLE 95 Segment location on transcripts Segment Segment Transcript name starting position ending position T46984_PEA_1_T2 3438 3451 T46984_PEA_1_T3 2832 2845 (SEQ ID NO: 315) T46984_PEA_1_T12 2232 2245 (SEQ ID NO: 316) T46984_PEA_1_T13 2263 2276 (SEQ ID NO: 317) T46984_PEA_1_T15 2121 2134 (SEQ ID NO: 319) T46984_PEA_1_T19 4066 4079 (SEQ ID NO: 320) T46984_PEA_1_T23 3342 3355 (SEQ ID NO: 321) T46984_PEA_1_T27 2022 2035 (SEQ ID NO: 322) T46984_PEA_1_T32 2028 2041 (SEQ ID NO: 323) T46984_PEA_1_T34 1774 1787 (SEQ ID NO: 324) T46984_PEA_1_T35 1932 1945 (SEQ ID NO: 325) T46984_PEA_1_T43 1210 1223 (SEQ ID NO: 328) T46984_PEA_1_T46 1524 1537 (SEQ ID NO: 329) T46984_PEA_1_T47 963 976 (SEQ ID NO: 330) T46984_PEA_1_T51 560 573 (SEQ ID NO: 332) T46984_PEA_1_T52 1063 1076 (SEQ ID NO: 333) T46984_PEA_1_T54 1063 1076 (SEQ ID NO: 334)

Segment cluster T46984_PEA_(—)1_node_(—)85 (SEQ ID NO: 383) according to the present invention is supported by 295 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): T46984_PEA_(—)1_T2, T46984_PEA_(—)1_T3 (SEQ ID NO: 315), T46984_PEA_(—)1_T12 (SEQ ID NO: 316), T46984_PEA_(—)1_T13 (SEQ ID NO: 317), T46984_PEA_(—)1_T15 (SEQ ID NO: 319), T46984_PEA_(—)1_T19 (SEQ ID NO: 320), T46984_PEA_(—)1_T23 (SEQ ID NO: 321), T46984_PEA_(—)1_T27 (SEQ ID NO: 322), T46984_PEA_(—)1_T32 (SEQ ID NO: 323), T46984_PEA_(—)1_T34 (SEQ ID NO: 324), T46984_PEA_(—)1_T35 (SEQ ID NO: 325), T46984_PEA_(—)1_T43 (SEQ ID NO: 328), T46984_PEA_(—)1_T46 (SEQ ID NO: 329), T46984_PEA_(—)1_T47 (SEQ ID NO: 330), T46984_PEA_(—)1_T51 (SEQ ID NO: 332), T46984_PEA_(—)1_T52 (SEQ ID NO: 333) and T46984_PEA_(—)1_T54 (SEQ ID NO: 334). Table 96 below describes the starting and ending position of this segment on each transcript.

TABLE 96 Segment location on transcripts Segment Segment Transcript name starting position ending position T46984_PEA_1_T2 3452 3491 T46984_PEA_1_T3 2846 2885 (SEQ ID NO: 315) T46984_PEA_1_T12 2246 2285 (SEQ ID NO: 316) T46984_PEA_1_T13 2277 2316 (SEQ ID NO: 317) T46984_PEA_1_T15 2135 2174 (SEQ ID NO: 319) T46984_PEA_1_T19 4080 4119 (SEQ ID NO: 320) T46984_PEA_1_T23 3356 3395 (SEQ ID NO: 321) T46984_PEA_1_T27 2036 2075 (SEQ ID NO: 322) T46984_PEA_1_T32 2042 2081 (SEQ ID NO: 323) T46984_PEA_1_T34 1788 1827 (SEQ ID NO: 324) T46984_PEA_1_T35 1946 1985 (SEQ ID NO: 325) T46984_PEA_1_T43 1224 1263 (SEQ ID NO: 328) T46984_PEA_1_T46 1538 1577 (SEQ ID NO: 329) T46984_PEA_1_T47 977 1016 (SEQ ID NO: 330) T46984_PEA_1_T51 574 613 (SEQ ID NO: 332) T46984_PEA_1_T52 1077 1116 (SEQ ID NO: 333) T46984_PEA_1_T54 1077 1116 (SEQ ID NO: 334) Variant protein alignment to the previously known protein: Sequence name: RIB2_HUMAN Sequence documentation:

Alignment of: T46984_PEA_(—)1_P2 x RIB2_HUMAN ..

Alignment segment 1/1:

Quality: 4716.00 Escore: 0 Matching length: 498 Total length: 498 Matching Percent Similarity: 100.00 Matching Percent Identity: 100.00 Total Percent Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0

Alignment:

Sequence name: RIB2_HUMAN Sequence documentation:

Alignment of: T46984_PEA_(—)1_P3 x RIB2_HUMAN ..

Alignment segment 1/1:

Quality: 4085.00 Escore: 0 Matching length: 433 Total length: 433 Matching Percent Similarity: 100.00 Matching Percent Identity: 100.00 Total Percent Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0

Alignment:

Sequence name: RIB2_HUMAN Sequence documentation:

Alignment of: T46984_PEA_(—)1_P10 (SEQ ID NO: 387) x RIB2_HUMAN ..

Alignment segment 1/1:

Quality: 4716.00 Escore: 0 Matching length: 498 Total length: 498 Matching Percent Similarity: 100.00 Matching Percent Identity: 100.00 Total Percent Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0

Alignment:

Sequence name: RIB2_HUMAN Sequence documentation:

Alignment of: T46984_PEA_(—)1_P11 (SEQ ID NO: 388) x RIB2_HUMAN ..

Alignment segment 1/1:

Quality: 5974.00 Escore: 0 Matching length: 628 Total length: 628 Matching Percent Similarity: 100.00 Matching Percent Identity: 100.00 Total Percent Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0

Alignment:

Sequence name: RIB2_HUMAN Sequence documentation:

Alignment of: T46984_PEA_(—)1_P12 (SEQ ID NO: 389) x RIB2_HUMAN ..

Alignment segment 1/1:

Quality: 3179.00 Escore: 0 Matching length: 338 Total length: 338 Matching Percent Similarity: 100.00 Matching Percent Identity: 100.00 Total Percent Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0

Alignment:

Sequence name: RIB2_HUMAN Sequence documentation:

Alignment of: T46984_PEA_(—)1_P21 (SEQ ID NO. 390 x RIB2_HUMAN ..

Alignment segment 1/1:

Quality: 5348.00 Escore: 0 Matching length: 562 Total length: 562 Matching Percent Similarity: 100.00 Matching Percent Identity: 100.00 Total Percent Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0

Alignment:

Sequence name: RIB2_HUMAN Sequence documentation:

Alignment of: T46984_PEA_(—)1_P27 (SEQ ID NO: 391) x RIB2_HUMAN ..

Alignment segment 1/1:

Quality: 3910.00 Escore: 0 Matching length: 415 Total length: 415 Matching Percent Similarity: 100.00 Matching Percent Identity: 100.00 Total Percent Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0

Alignment:

Sequence name: RIB2_HUMAN Sequence documentation:

Alignment of: T46984_PEA_(—)1_P32 (SEQ ID NO: 392) x RIB2_HUMAN ..

Alignment segment 1/1:

Quality: 3434.00 Escore: 0 Matching length: 373 Total length: 373 Matching Percent Similarity: 98.93 Matching Percent Identity: 98.39 Total Percent Similarity: 98.93 Total Percent Identity: 98.39 Gaps: 0

Alignment:

Sequence name: RIB2_HUMAN Sequence documentation:

Alignment of: T46984_PEA_(—)1_P34 (SEQ ID NO: 393) x RIB2_HUMAN ..

Alignment segment 1/1:

Quality: 3087.00 Escore: 0 Matching length: 329 Total length: 329 Matching Percent Similarity: 100.00 Matching Percent Identity: 100.00 Total Percent Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0

Alignment:

Sequence name: RIB2_HUMAN Sequence documentation:

Alignment of: T46984_PEA_(—)1_P35 (SEQ ID NO: 394) x RIB2_HUMAN ..

Alignment segment 1/1:

Quality: 2697.00 Escore: 0 Matching length: 287 Total length: 287 Matching Percent Similarity: 100.00 Matching Percent Identity: 100.00 Total Percent Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0

Alignment:

Sequence name: RIB2_HUMAN Sequence documentation:

Alignment of: T46984_PEA_(—)1_P38 (SEQ ID NO: 395) x RIB2_HUMAN ..

Alignment segment 1/1:

Quality: 1368.00 Escore: 0 Matching length: 145 Total length: 145 Matching Percent Similarity: 100.00 Matching Percent Identity: 100.00 Total Percent Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0

Alignment:

Sequence name: RIB2_HUMAN Sequence documentation:

Alignment of: T46984_PEA_(—)1_P39 (SEQ ID NO: 396) x RIB2_HUMAN ..

Alignment segment 1/1:

Quality: 1500.00 Escore: 0 Matching length: 160 Total length: 160 Matching Percent Similarity: 100.00 Matching Percent Identity: 100.00 Total Percent Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0

Alignment:

Sequence name: RIB2_HUMAN Sequence documentation:

Alignment of: T46984_PEA_(—)1_P45 (SEQ ID NO: 397) x RIB2_HUMAN ..

Alignment segment 1/1:

Quality: 970.00 Escore: 0 Matching length: 103 Total length: 103 Matching Percent Similarity: 99.03 Matching Percent Identity: 99.03 Total Percent Similarity: 99.03 Total Percent Identity: 99.03 Gaps: 0

Alignment:

Sequence name: RIB2_HUMAN Sequence documentation:

Alignment of: T46984_PEA_(—)1_P46 (SEQ ID NO: 398) x RIB2_HUMAN ..

Alignment segment 1/1:

Quality: 656.00 Escore: 0 Matching length: 69 Total length: 69 Matching Percent Similarity: 100.00 Matching Percent Identity: 100.00 Total Percent Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0

Alignment:

Description for Cluster M78530

Cluster M78530 features 3 transcript(s) and 21 segment(s) of interest, the names for which are given in Tables 1 and 2, respectively, the sequences themselves are given at the end of the application. The selected protein variants are given in table 3.

TABLE 1 Transcripts of interest SEQ ID Transcript Name NO: M78530_PEA_1_T11 399 M78530_PEA_1_T12 400 M78530_PEA_1_T13 401

TABLE 2 Segments of interest SEQ ID Segment Name NO: M78530_PEA_1_node_0 402 M78530_PEA_1_node_15 403 M78530_PEA_1_node_16 404 M78530_PEA_1_node_19 405 M78530_PEA_1_node_21 406 M78530_PEA_1_node_23 407 M78530_PEA_1_node_27 408 M78530_PEA_1_node_29 409 M78530_PEA_1_node_36 410 M78530_PEA_1_node_37 411 M78530_PEA_1_node_2 412 M78530_PEA_1_node_4 413 M78530_PEA_1_node_5 414 M78530_PEA_1_node_7 415 M78530_PEA_1_node_9 416 M78530_PEA_1_node_10 417 M78530_PEA_1_node_18 418 M78530_PEA_1_node_25 419 M78530_PEA_1_node_30 420 M78530_PEA_1_node_33 421 M78530_PEA_1_node_34 422

TABLE 3 Proteins of interest SEQ ID Corresponding Protein Name NO: Transcript(s) M78530_PEA_1_P15 426 M78530_PEA_1_T11 (SEQ ID NO:399) M78530_PEA_1_P16 427 M78530_PEA_1_T12 (SEQ ID NO.400) M78530_PEA_1_P17 428 M78530_PEA_1_T13 (SEQ ID NO:401)

Cluster M78530 can be used as a diagnostic marker according to overexpression of transcripts of this cluster in cancer. Expression of such transcripts in normal tissues is also given according to the previously described methods. The term “number” in the left hand column of the table and the numbers on the y-axis of FIG. 40 refer to weighted expression of ESTs in each category, as “parts per million” (ratio of the expression of ESTs for a particular cluster to the expression of all ESTs in that category, according to parts per million).

Overall, the following results were obtained as shown with regard to the histograms in FIG. 40 and Table 4. This cluster is overexpressed (at least at a minimum level) in the following pathological conditions: ovarian carcinoma.

TABLE 4 Normal tissue distribution Name of Tissue Number adrenal 40 bladder 41 brain 52 colon 126 epithelial 51 general 35 kidney 199 lung 63 breast 0 ovary 0 pancreas 20 prostate 28 stomach 0 uterus 113

TABLE 5 P values and ratios for expression in cancerous tissue Name of Tissue P1 P2 SP1 R3 SP2 R4 adrenal 6.4e−01 6.9e−01 7.1e−01 1.1 7.8e−01 0.9 bladder 3.3e−01 4.5e−01 2.8e−01 2.0 4.9e−01 1.4 brain 7.9e−01 8.1e−01 8.5e−01 0.6 9.8e−01 0.4 colon 4.7e−01 6.1e−01 9.7e−01 0.5 9.9e−01 0.4 epithelial 2.0e−01 8.2e−01 3.3e−03 1.6 2.5e−01 0.9 general 1.3e−01 8.5e−01 7.4e−10 2.2 6.0e−04 1.4 kidney 7.0e−01 7.6e−01 1 0.2 1 0.1 lung 8.6e−01 9.1e−01 1 0.3 1 0.3 breast 1.9e−01 2.8e−01 3.3e−01 2.4 5.6e−01 1.6 ovary 1.6e−02 1.3e−02 7.0e−05 10.3 6.3e−06 9.3 pancreas 2.6e−01 4.1e−01 3.5e−02 2.2 1.2e−01 1.5 prostate 7.9e−01 8.6e−01 4.7e−01 1.2 6.3e−01 1.0 stomach 1.1e−01 4.5e−01 5.0e−01 2.2 8.0e−01 1.3 uterus 5.3e−01 8.2e−01 2.4e−01 1.0 7.7e−01 0.6

For this cluster, at least one oligonucleotide was found to demonstrate overexpression of the cluster, although not of at least one transcript/segment as listed below. Microarray (chip) data is also available for this cluster as follows. Various oligonucleotides were tested for being differentially expressed in various disease conditions, particularly cancer, as previously described. The following oligonucleotides were found to hit this cluster but not other segments/transcripts below (in relation to ovarian cancer), shown in Table 6.

TABLE 6 Oligonucleotides related to this cluster Oligonucleotide name Overexpressed in cancers Chip reference M78530_0_6_0 (SEQ ID ovarian carcinoma OVA NO: 1027)

As noted above, cluster M78530 features 3 transcript(s), which were listed in Table 1 above. A description of each variant protein according to the present invention is now provided.

Variant protein M78530_PEA_(—)1_P15 (SEQ ID NO: 426) according to the present invention has an amino acid sequence as given at the end of the application; it is encoded by transcript(s) M78530_PEA_(—)1_T11 (SEQ ID NO: 399). One or more alignments to one or more previously published protein sequences are given at the end of the application. A brief description of the relationship of the variant protein according to the present invention to each such aligned protein is as follows:

Comparison report between M78530_PEA_(—)1_P15 (SEQ ID NO: 426) and Q9HCB6 (SEQ ID NO:424):

1. An isolated chimeric polypeptide encoding for M78530_PEA_(—)1_P15 (SEQ ID NO: 426), comprising a first amino acid sequence being at least 90% homologous to MRLSPAPLKLSRTPALLALALPLAAALAFSDETLDKVPKSEGYCSRILRAQGTRREGYT EFSLRVEGDPDFYKPGTSYRVTLSAAPPSYFRGFTLIALRENREGDKEEDHAGTFQIIDEE ETQFMSNCPVAVTESTPRRRTRIQVFWIAPPAGTGCVILKASIVQKRIIYFQDEGSLTKKL CEQDSTFDGVTDKPILDCCACGTAKYRLTFYGNWSEKTHPKDYPRRANHWSAIIGGSH SKNYVLWEYGGYASEGVKQVAELGSPVKMEEEIRQQSDEVLTVIKAKAQWPAWQPLN VRAAPSAEFSVDRTRHLMSFLTMMGPSPDWNVGLSAEDLCTKECGWVQKVVQDLIPW DAGTDSGVTYESPNKPTIPQEKIRPLTSLDHPQSPFYDPEGGSITQVARVVIERIARKGEQ CNIVPDNVDDIVADLAPEEKDEDDTPETCIYSNWSPWSACSSSTCDKGKRMRQRMLKA QLDLSVPCPDTQDFQPCMGPGCSDEDGSTCTMSEWITWSPCSISCGMGMRSRERYVKQ FPEDGSVCTLPTEE corresponding to amino acids 1-544 of Q9HCB6, which also corresponds to amino acids 1-544 of M78530_PEA_(—)1_P15 (SEQ ID NO: 426), a bridging amino acid T corresponding to amino acid 545 of M78530_PEA_(—)1_P15 (SEQ ID NO: 426), a second amino acid sequence being at least 90% homologous to EKCTVNEECSPSSCLMTEWGEWDECSATCGMGMKKRHRMIKMNPADGSMCKAETSQ AEKCMMPECHTIPCLLSPWSEWSDCSVTCGKGMRTRQRMLKSLAELGDCNEDLEQVE KCMLPEC corresponding to amino acids 546-665 of Q9HCB6, which also corresponds to amino acids 546-665 of M78530_PEA_(—)1_P15 (SEQ ID NO: 426), and a third amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence RKSWSSSRPITSMFLSPGSPEPASANTARS (SEQ ID NO: 1070) corresponding to amino acids 666-695 of M78530_PEA_(—)1_P15 (SEQ ID NO: 426), wherein said first amino acid sequence, bridging amino acid, second amino acid sequence and third amino acid sequence are contiguous and in a sequential order.

2. An isolated polypeptide encoding for a tail of M78530_PEA_(—)1_P15 (SEQ ID NO: 426), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence RKSWSSSRPITSMFLSPGSPEPASANTARS (SEQ ID NO: 1070) in M78530_PEA_(—)1_P15 (SEQ ID NO: 426).

Comparison report between M78530_PEA_(—)1_P15 (SEQ ID NO: 426) and O94862 (SEQ ID NO:425):

1. An isolated chimeric polypeptide encoding for M78530_PEA_(—)1_P15 (SEQ ID NO: 426), comprising a first amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence MRLSPAPLKLSRTPALLALALPLAAALAFSDETLDKVPKSEGYCSRILRAQGTRREGYT EFSLRVEGDPDFYKPGTSYRVTLS (SEQ ID NO: 1071) corresponding to amino acids 1-83 of M78530_PEA_(—)1_P15 (SEQ ID NO: 426), a second amino acid sequence being at least 90% homologous to AAPPSYFRGFTLIALRENREGDKEEDHAGTFQIIDEEETQFMSNCPVAVTESTPRRRTRIQ VFWIAPPAGTGCVILKASIVQKRIIYFQDEGSLTKKLCEQDSTFDGVTDKPILDCCACGT AKYRLTFYGNWSEKTHPKDYPRRANHWSAIIGGSHSKNYVLWEYGGYASEGVKQVAE LGSPVKMEEEIRQQSDEVLTVIKAKAQWPAWQPLNVRAAPSAEFSVDRTRHLMSFLTM MGPSPDWNVGLSAEDLCTKECGWVQKVVQDLIPWDAGTDSGVTYESPNKPTIPQEKIR PLTSLDHPQSPFYDPEGGSITQVARVVIERIARKGEQCNIVPDNVDDIVADLAPEEKDED DTPETCIYSNWSPWSACSSSTCDKGKRMRQRMLKAQLDLSVPCPDTQDFQPCMGPGCS DEDGSTCTMSEWITWSPCSISCGMGMRSRERYVKQFPEDGSVCTLPTEETEKCTVNEEC SPSSCLMTEWGEWDECSATCGMGMKKRHRMIKMNPADGSMCKAETSQAEKCMMPE CHTIPCLLSPWSEWSDCSVTCGKGMRTRQRMLKSLAELGDCNEDLEQVEKCMLPEC corresponding to amino acids 1-582 of O94862, which also corresponds to amino acids 84-665 of M78530_PEA_(—)1_P15 (SEQ ID NO: 426), and a third amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence RKSWSSSRPITSMFLSPGSPEPASANTARS (SEQ ID NO: 1070) corresponding to amino acids 666-695 of M78530_PEA_(—)1_P15 (SEQ ID NO: 426), wherein said first amino acid sequence, second amino acid sequence and third amino acid sequence are contiguous and in a sequential order.

2. An isolated polypeptide encoding for a head of M78530_PEA_(—)1_P15 (SEQ ID NO: 426), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence MRLSPAPLKLSRTPALLALALPLAAALAFSDETLDKVPKSEGYCSRILRAQGTRREGYT EFSLRVEGDPDFYKPGTSYRVTLS (SEQ ID NO: 1071) of M78530_PEA_(—)1_P15 (SEQ ID NO: 426).

3. An isolated polypeptide encoding for a tail of M78530_PEA_(—)1_P15 (SEQ ID NO: 426), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence RKSWSSSRPITSMFLSPGSPEPASANTARS (SEQ ID NO: 1070) in M78530_PEA_(—)1_P15 (SEQ ID NO: 426).

The location of the variant protein was determined according to results from a number of different software programs and analyses, including analyses from SignalP and other specialized programs. The variant protein is believed to be located as follows with regard to the cell: secreted. The protein localization is believed to be secreted because both signal-peptide prediction programs predict that this protein has a signal peptide, and neither trans-membrane region prediction program predicts that this protein has a trans-membrane region.

Variant protein M78530_PEA_(—)1_P15 (SEQ ID NO: 426) also has the following non-silent SNPs (Single Nucleotide Polymorphisms) as listed in Table 7, (given according to their position(s) on the amino acid sequence, with the alternative amino acid(s) listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein M78530_PEA_(—)1_P15 (SEQ ID NO: 426) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 7 Amino acid mutations SNP position(s) on amino acid sequence Alternative amino acid(s) Previously known SNP? 278 E -> D No 278 E -> V No

Variant protein M78530_PEA_(—)1_P15 (SEQ ID NO: 426) is encoded by the following transcript(s): M78530_PEA_(—)1_T11 (SEQ ID NO: 399), for which the sequence(s) is/are given at the end of the application. The coding portion of transcript M78530_PEA_(—)1_T11 (SEQ ID NO: 399) is shown in bold; this coding portion starts at position 629 and ends at position 2713. The transcript also has the following SNPs as listed in Table 8 (given according to their position on the nucleotide sequence, with the alternative nucleic acid listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein M78530_PEA_(—)1_P15 (SEQ ID NO: 426) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 8 Nucleic acid SNPs SNP position on nucleotide sequence Alternative nucleic acid Previously known SNP? 760 C -> T No 1461 A -> T No 1462 G -> T No 1492 A -> G No

Variant protein M78530_PEA_(—)1_P16 (SEQ ID NO: 427) according to the present invention has an amino acid sequence as given at the end of the application; it is encoded by transcript(s) M78530_PEA_(—)1_T12 (SEQ ID NO. 400). One or more alignments to one or more previously published protein sequences are given at the end of the application. A brief description of the relationship of the variant protein according to the present invention to each such aligned protein is as follows:

Comparison report between M78530_PEA_(—)1_P16 (SEQ ID NO: 427) and Q8NCD7 (SEQ ID NO: 423):

1. An isolated chimeric polypeptide encoding for M78530_PEA_(—)1_P16 (SEQ ID NO: 427), comprising a first amino acid sequence being at least 90% homologous to MRLSPAPLKLSRTPALLALALPLAAALAFSDETLDKVPKSEGYCSRILRAQGTRREGYT EFSLRVEGDPDFYKPGTSYRVTLSAAPPSYFRGFTLIALRENREGDKEEDHAGTFQIIDEE ETQFMSNCPVAVTESTPRRRTRIQVFWIAPPAGTGCVILKASIVQKRIIYFQDEGSLTKKL CEQDSTFDGVTDKPILDCCACGTAKYRLTFYGNWSEKTHPKDYPRRANHWSAIIGGSH SKNYVLWEYGGYASEGVKQVAELGSPVKMEEEIRQQSDEVLTVIKAKAQWPAWQPLN V corresponding to amino acids 1-297 of Q8NCD7, which also corresponds to amino acids 1-297 of M78530_PEA_(—)1_P16 (SEQ ID NO: 427).

Comparison report between M78530_PEA_(—)1_P16 (SEQ ID NO: 427) and Q9HCB6 (SEQ ID NO: 424):

1. An isolated chimeric polypeptide encoding for M78530_PEA_(—)1_P16 (SEQ ID NO: 427), comprising a first amino acid sequence being at least 90% homologous to MRLSPAPLKLSRTPALLALALPLAAALAFSDETLDKVPKSEGYCSRILRAQGTRREGYT EFSLRVEGDPDFYKPGTSYRVTLSAAPPSYFRGFTLIALRENREGDKEEDHAGTFQIIDEE ETQFMSNCPVAVTESTPRRRTRIQVFWIAPPAGTGCVILKASIVQKRIIYFQDEGSLTKKL CEQDSTFDGVTDKPILDCCACGTAKYRLTFYGNWSEKTHPKDYPRRANHWSAIIGGSH SKNYVLWEYGGYASEGVKQVAELGSPVKMEEEIRQQSDEVLTVIKAKAQWPAWQPLN V corresponding to amino acids 1-297 of Q9HCB6, which also corresponds to amino acids 1-297 of M78530_PEA_(—)1_P16 (SEQ ID NO: 427).

Comparison report between M78530_PEA_(—)1_P16 (SEQ ID NO: 427) and O94862 (SEQ ID NO: 425):

1. An isolated chimeric polypeptide encoding for M78530_PEA_(—)1_P16 (SEQ ID NO: 427), comprising a first amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence MRLSPAPLKLSRTPALLALALPLAAALAFSDETLDKVPKSEGYCSRILRAQGTRREGYT EFSLRVEGDPDFYKPGTSYRVTLS (SEQ ID NO: 1071) corresponding to amino acids 1-83 of M78530_PEA_(—)1_P16 (SEQ ID NO: 427), and a second amino acid sequence being at least 90% homologous to AAPPSYFRGFTLIALRENREGDKEEDHAGTFQIIDEEETQFMSNCPVAVTESTPRRRTRIQ VFWIAPPAGTGCVILKASIVQKRIIYFQDEGSLTKKLCEQDSTFDGVTDKPILDCCACGT AKYRLTFYGNWSEKTHPKDYPRRANHWSAIIGGSHSKNYVLWEYGGYASEGVKQVAE LGSPVKMEEEIRQQSDEVLTVIKAKAQWPAWQPLNV corresponding to amino acids 1-214 of O94862, which also corresponds to amino acids 84-297 of M78530_PEA_(—)1_P16 (SEQ ID NO: 427), wherein said first amino acid sequence and second amino acid sequence are contiguous and in a sequential order.

2. An isolated polypeptide encoding for a head of M78530_PEA_(—)1_P16 (SEQ ID NO: 427), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence MRLSPAPLKLSRTPALLALALPLAAALAFSDETLDKVPKSEGYCSRILRAQGTRREGYT EFSLRVEGDPDFYKPGTSYRVTLS (SEQ ID NO: 1071) of M78530_PEA_(—)1_P16 (SEQ ID NO: 427).

The location of the variant protein was determined according to results from a number of different software programs and analyses, including analyses from SignalP and other specialized programs. The variant protein is believed to be located as follows with regard to the cell: secreted. The protein localization is believed to be secreted because both signal-peptide prediction programs predict that this protein has a signal peptide, and neither trans-membrane region prediction program predicts that this protein has a trans-membrane region.

Variant protein M78530_PEA_(—)1_P16 (SEQ ID NO: 427) also has the following non-silent SNPs (Single Nucleotide Polymorphisms) as listed in Table 9, (given according to their position(s) on the amino acid sequence, with the alternative amino acid(s) listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein M78530_PEA_(—)1_P16 (SEQ ID NO: 427) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 9 Amino acid mutations SNP position(s) on amino acid sequence Alternative amino acid(s) Previously known SNP? 278 E -> D No 278 E -> V No

Variant protein M78530_PEA_(—)1_P16 (SEQ ID NO: 427) is encoded by the following transcript(s): M78530_PEA_(—)1_T12 (SEQ ID NO. 400), for which the sequence(s) is/are given at the end of the application. The coding portion of transcript M78530_PEA_(—)1_T12 (SEQ ID NO. 400) is shown in bold; this coding portion starts at position 629 and ends at position 1519. The transcript also has the following SNPs as listed in Table 10 (given according to their position on the nucleotide sequence, with the alternative nucleic acid listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein M78530_PEA_(—)1_P16 (SEQ ID NO: 427) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 10 Nucleic acid SNPs SNP position on nucleotide sequence Alternative nucleic acid Previously known SNP? 760 C -> T No 1461 A -> T No 1462 G -> T No 1492 A -> G No 1670 T -> C No 1957 T -> C No 2004 A -> C No 2005 A -> T No

Variant protein M78530_PEA_(—)1_P17 (SEQ ID NO: 428) according to the present invention has an amino acid sequence as given at the end of the application; it is encoded by transcript(s) M78530_PEA_(—)1_T13 (SEQ ID NO: 401). One or more alignments to one or more previously published protein sequences are given at the end of the application. A brief description of the relationship of the variant protein according to the present invention to each such aligned protein is as follows:

Comparison report between M78530_PEA_(—)1_P17 (SEQ ID NO: 428) and Q8NCD7:

1. An isolated chimeric polypeptide encoding for M78530_PEA_(—)1_P17 (SEQ ID NO: 428), comprising a first amino acid sequence being at least 90% homologous to MRLSPAPLKLSRTPALLALALPLAAALAFSDETLDKVPKSEGYCSRILRAQGTRREGYT EFSLRVEGDPDFYKPGTSYRVTLSAAPPSYFRGFTLIALRENREGDKEEDHAGTFQIIDEE ETQFMSNCPVAVTESTPRRRTRIQVFWIAPPAGTGCVILKASIVQKRIIYFQDEGSLTKKL CEQDSTFDGVTDKPILDCCACGTAKYRLTFYGNWSEKTHPKDYPRRANHWSAIIGGSH SKNYVLWEYGGYASEGVKQVAELGSPVKMEEEIRQQ corresponding to amino acids 1-275 of Q8NCD7, which also corresponds to amino acids 1-275 of M78530_PEA_(—)1_P17 (SEQ ID NO: 428), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence VRQKNHRMTK (SEQ ID NO: 1073) corresponding to amino acids 276-285 of M78530_PEA_(—)1_P17 (SEQ ID NO: 428), wherein said first amino acid sequence and second amino acid sequence are contiguous and in a sequential order.

2. An isolated polypeptide encoding for a tail of M78530_PEA_(—)1_P17 (SEQ ID NO: 428), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence VRQKNHRMTK (SEQ ID NO: 1073) in M78530_PEA_(—)1_P17 (SEQ ID NO: 428).

Comparison report between M78530_PEA_(—)1_P17 (SEQ ID NO: 428) and Q9HCB6:

1. An isolated chimeric polypeptide encoding for M78530_PEA_(—)1_P17 (SEQ ID NO: 428), comprising a first amino acid sequence being at least 90% homologous to MRLSPAPLKLSRTPALLALALPLAAALAFSDETLDKVPKSEGYCSRILRAQGTRREGYT EFSLRVEGDPDFYKPGTSYRVTLSAAPPSYFRGFTLIALRENREGDKEEDHAGTFQIIDEE ETQFMSNCPVAVTESTPRRRTRIQVFWIAPPAGTGCVILKASIVQKRIIYFQDEGSLTKKL CEQDSTFDGVTDKPILDCCACGTAKYRLTFYGNWSEKTHPKDYPRRANHWSAIIGGSH SKNYVLWEYGGYASEGVKQVAELGSPVKMEEEIRQQ corresponding to amino acids 1-275 of Q9HCB6, which also corresponds to amino acids 1-275 of M78530_PEA_(—)1_P17 (SEQ ID NO: 428), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence VRQKNHRMTK (SEQ ID NO: 1073) corresponding to amino acids 276-285 of M78530_PEA_(—)1_P17 (SEQ ID NO: 428), wherein said first amino acid sequence and second amino acid sequence are contiguous and in a sequential order.

2. An isolated polypeptide encoding for a tail of M78530_PEA_(—)1_P17 (SEQ ID NO: 428), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence VRQKNHRMTK (SEQ ID NO: 1073) in M78530_PEA_(—)1_P17 (SEQ ID NO: 428).

Comparison report between M78530_PEA_(—)1_P17 (SEQ ID NO: 428) and O94862:

1. An isolated chimeric polypeptide encoding for M78530_PEA_(—)1_P17 (SEQ ID NO: 428), comprising a first amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence MRLSPAPLKLSRTPALLALALPLAAALAFSDETLDKVPKSEGYCSRILRAQGTRREGYT EFSLRVEGDPDFYKPGTSYRVTLS (SEQ ID NO: 1071) corresponding to amino acids 1-83 of M78530_PEA_(—)1_P17 (SEQ ID NO: 428), a second amino acid sequence being at least 90% homologous to AAPPSYFRGFTLIALRENREGDKEEDHAGTFQIIDEEETQFMSNCPVAVTESTPRRRTRIQ VFWIAPPAGTGCVILKASIVQKRIIYFQDEGSLTKKLCEQDSTFDGVTDKPILDCCACGT AKYRLTFYGNWSEKTHPKDYPRRANHWSAIIGGSHSKNYVLWEYGGYASEGVKQVAE LGSPVKMEEEIRQQ corresponding to amino acids 1-192 of O94862, which also corresponds to amino acids 84-275 of M78530_PEA_(—)1_P17 (SEQ ID NO: 428), and a third amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence VRQKNHRMTK (SEQ ID NO: 1073) corresponding to amino acids 276-285 of M78530_PEA_(—)1_P17 (SEQ ID NO: 428), wherein said first amino acid sequence, second amino acid sequence and third amino acid sequence are contiguous and in a sequential order.

2. An isolated polypeptide encoding for a head of M78530_PEA_(—)1_P17 (SEQ ID NO: 428), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence MRLSPAPLKLSRTPALLALALPLAAALAFSDETLDKVPKSEGYCSRILRAQGTRREGYT EFSLRVEGDPDFYKPGTSYRVTLS (SEQ ID NO: 1071) of M78530_PEA_(—)1_P17 (SEQ ID NO: 428).

3. An isolated polypeptide encoding for a tail of M78530_PEA_(—)1_P17 (SEQ ID NO: 428), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence VRQKNHRMTK (SEQ ID NO: 1073) in M78530_PEA_(—)1_P17 (SEQ ID NO: 428).

The location of the variant protein was determined according to results from a number of different software programs and analyses, including analyses from SignalP and other specialized programs. The variant protein is believed to be located as follows with regard to the cell: secreted. The protein localization is believed to be secreted because both signal-peptide prediction programs predict that this protein has a signal peptide, and neither trans-membrane region prediction program predicts that this protein has a trans-membrane region.

Variant protein M78530_PEA_(—)1_P17 (SEQ ID NO: 428) is encoded by the following transcript(s): M78530_PEA_(—)1_T13 (SEQ ID NO: 401), for which the sequence(s) is/are given at the end of the application. The coding portion of transcript M78530_PEA_(—)1_T13 (SEQ ID NO: 401) is shown in bold; this coding portion starts at position 629 and ends at position 1483. The transcript also has the following SNPs as listed in Table 11 (given according to their position on the nucleotide sequence, with the alternative nucleic acid listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein M78530_PEA_(—)1_P17 (SEQ ID NO: 428) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 11 Nucleic acid SNPs SNP position on nucleotide sequence Alternative nucleic acid Previously known SNP? 760 C -> T No

As noted above, cluster M78530 features 21 segment(s), which were listed in Table 2 above and for which the sequence(s) are given at the end of the application. These segment(s) are portions of nucleic acid sequence(s) which are described herein separately because they are of particular interest. A description of each segment according to the present invention is now provided.

Segment cluster M78530_PEA_(—)1_node_(—)0 (SEQ ID NO: 402) according to the present invention is supported by 21 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): M78530_PEA_(—)1_T11 (SEQ ID NO: 399), M78530_PEA_(—)1_T12 (SEQ ID NO. 400) and M78530_PEA_(—)1_T13 (SEQ ID NO: 401). Table 12 below describes the starting and ending position of this segment on each transcript.

TABLE 12 Segment location on transcripts Segment Segment starting ending Transcript name position position M78530_PEA_1_T11 1 866 (SEQ ID NO:399) M78530_PEA_1_T12 1 866 (SEQ ID NO.400) M78530_PEA_1_T13 1 866 (SEQ ID NO:401)

Segment cluster M78530_PEA_(—)1_node_(—)15 (SEQ ID NO: 403) according to the present invention is supported by 27 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): M78530_PEA_(—)1_T11 (SEQ ID NO: 399), M78530_PEA_(—)1_T12 (SEQ ID NO. 400) and M78530_PEA_(—)1_T13 (SEQ ID NO: 401). Table 13 below describes the starting and ending position of this segment on each transcript.

TABLE 13 Segment location on transcripts Segment Segment starting ending Transcript name position position M78530_PEA_1_T11 1305 1453 (SEQ ID NO:399) M78530_PEA_1_T12 1305 1453 (SEQ ID NO.400) M78530_PEA_1_T13 1305 1453 (SEQ ID NO:401)

Segment cluster M78530_PEA_(—)1_node_(—)16 (SEQ ID NO: 404) according to the present invention is supported by 1 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): M78530_PEA_(—)1_T13 (SEQ ID NO: 401). Table 14 below describes the starting and ending position of this segment on each transcript.

TABLE 14 Segment location on transcripts Segment Segment starting ending Transcript name position position M78530_PEA_1_T13 1454 1593 (SEQ ID NO:401)

Segment cluster M78530_PEA_(—)1_node_(—)19 (SEQ ID NO: 405) according to the present invention is supported by 2 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): M78530_PEA_(—)1_T12 (SEQ ID NO. 400). Table 15 below describes the starting and ending position of this segment on each transcript.

TABLE 15 Segment location on transcripts Segment Segment starting ending Transcript name position position M78530_PEA_1_T12 1519 2461 (SEQ ID NO:400)

Segment cluster M78530_PEA_(—)1_node_(—)21 (SEQ ID NO: 406) according to the present invention is supported by 27 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): M78530_PEA_(—)1_T11 (SEQ ID NO: 399). Table 16 below describes the starting and ending position of this segment on each transcript.

TABLE 16 Segment location on transcripts Segment Segment starting ending Transcript name position position M78530_PEA_1_T11 1519 1720 (SEQ ID NO:399)

Segment cluster M78530_PEA_(—)1_node_(—)23 (SEQ ID NO: 407) according to the present invention is supported by 22 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): M78530_PEA_(—)1_T11 (SEQ ID NO: 399). Table 17 below describes the starting and ending position of this segment on each transcript.

TABLE 17 Segment location on transcripts Segment Segment starting ending Transcript name position position M78530_PEA_1_T11 1721 1861 (SEQ ID NO:399)

Segment cluster M78530_PEA_(—)1_node_(—)27 (SEQ ID NO: 408) according to the present invention is supported by 26 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): M78530_PEA_(—)1_T11 (SEQ ID NO: 399). Table 18 below describes the starting and ending position of this segment on each transcript.

TABLE 18 Segment location on transcripts Segment Segment starting ending Transcript name position position M78530_PEA_1_T11 1938 2120 (SEQ ID NO:399)

Segment cluster M78530_PEA_(—)1_node_(—)29 (SEQ ID NO: 409) according to the present invention is supported by 34 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): M78530_PEA_(—)1_T11 (SEQ ID NO: 399). Table 19 below describes the starting and ending position of this segment on each transcript.

TABLE 19 Segment location on transcripts Segment Segment starting ending Transcript name position position M78530_PEA_1_T11 2121 2278 (SEQ ID NO:399)

Segment cluster M78530_PEA_(—)1_node_(—)36 (SEQ ID NO: 410) according to the present invention is supported by 41 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): M78530_PEA_(—)1_T11 (SEQ ID NO: 399). Table 20 below describes the starting and ending position of this segment on each transcript.

TABLE 20 Segment location on transcripts Segment Segment starting ending Transcript name position position M78530_PEA_1_T11 2460 2624 (SEQ ID NO:399)

Segment cluster M78530_PEA_(—)1_node_(—)37 (SEQ ID NO: 411) according to the present invention is supported by 1 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): M78530_PEA_(—)1_T11 (SEQ ID NO: 399). Table 21 below describes the starting and ending position of this segment on each transcript.

TABLE 21 Segment location on transcripts Segment Segment starting ending Transcript name position position M78530_PEA_1_T11 2625 2816 (SEQ ID NO:399)

According to an optional embodiment of the present invention, short segments related to the above cluster are also provided. These segments are up to about 120 bp in length, and so are included in a separate description.

Segment cluster M78530_PEA_(—)1_node_(—)2 (SEQ ID NO: 412) according to the present invention is supported by 21 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): M78530_PEA_(—)1_T11 (SEQ ID NO: 399), M78530_PEA_(—)1_T12 (SEQ ID NO. 400) and M78530_PEA_(—)1_T13 (SEQ ID NO: 401). Table 22 below describes the starting and ending position of this segment on each transcript.

TABLE 22 Segment location on transcripts Segment Segment starting ending Transcript name position position M78530_PEA_1_T11 867 973 (SEQ ID NO:399) M78530_PEA_1_T12 867 973 (SEQ ID NO.400) M78530_PEA_1_T13 867 973 (SEQ ID NO:401)

Segment cluster M78530_PEA_(—)1_node_(—)4 (SEQ ID NO: 413) according to the present invention is supported by 19 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): M78530_PEA_(—)1_T11 (SEQ ID NO: 399), M78530_PEA_(—)1_T12 (SEQ ID NO. 400) and M78530_PEA_(—)1_T13 (SEQ ID NO: 401). Table 23 below describes the starting and ending position of this segment on each transcript.

TABLE 23 Segment location on transcripts Segment Segment starting ending Transcript name position position M78530_PEA_1_T11 974 1025 (SEQ ID NO:399) M78530_PEA_1_T12 974 1025 (SEQ ID NO.400) M78530_PEA_1_T13 974 1025 (SEQ ID NO:401)

Segment cluster M78530_PEA_(—)1_node_(—)5 (SEQ ID NO: 414) according to the present invention is supported by 21 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): M78530_PEA_(—)1_T11 (SEQ ID NO: 399), M78530_PEA_(—)1_T12 (SEQ ID NO. 400) and M78530_PEA_(—)1_T13 (SEQ ID NO: 401). Table 24 below describes the starting and ending position of this segment on each transcript.

TABLE 24 Segment location on transcripts Segment Segment starting ending Transcript name position position M78530_PEA_1_T11 1026 1107 (SEQ ID NO:399) M78530_PEA_1_T12 1026 1107 (SEQ ID NO.400) M78530_PEA_1_T13 1026 1107 (SEQ ID NO:401)

Segment cluster M78530_PEA_(—)1_node_(—)7 (SEQ ID NO: 415) according to the present invention is supported by 23 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): M78530_PEA_(—)1_T11 (SEQ ID NO: 399), M78530_PEA_(—)1_T12 (SEQ ID NO. 400) and M78530_PEA_(—)1_T13 (SEQ ID NO: 401). Table 25 below describes the starting and ending position of this segment on each transcript.

TABLE 25 Segment location on transcripts Segment Segment Transcript name starting position ending position M78530_PEA_1_T11 1108 1181 (SEQ ID NO: 399) M78530_PEA_1_T12 1108 1181 (SEQ ID NO. 400) M78530_PEA_1_T13 1108 1181 (SEQ ID NO: 401)

Segment cluster M78530_PEA_(—)1_node_(—)9 (SEQ ID NO: 416) according to the present invention is supported by 24 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): M78530_PEA_(—)1_T11 (SEQ ID NO: 399), M78530_PEA_(—)1_T12 (SEQ ID NO. 400) and M78530_PEA_(—)1_T13 (SEQ ID NO: 401). Table 26 below describes the starting and ending position of this segment on each transcript.

TABLE 26 Segment location on transcripts Segment Segment Transcript name starting position ending position M78530_PEA_1_T11 1182 1288 (SEQ ID NO: 399) M78530_PEA_1_T12 1182 1288 (SEQ ID NO. 400) M78530_PEA_1_T13 1182 1288 (SEQ ID NO: 401)

Segment cluster M78530_PEA_(—)1_node_(—)10 (SEQ ID NO: 417) according to the present invention can be found in the following transcript(s): M78530_PEA_(—)1_T11 (SEQ ID NO: 399), M78530_PEA_(—)1_T12 (SEQ ID NO. 400) and M78530_PEA_(—)1_T13 (SEQ ID NO: 401). Table 27 below describes the starting and ending position of this segment on each transcript.

TABLE 27 Segment location on transcripts Segment Segment Transcript name starting position ending position M78530_PEA_1_T11 1289 1304 (SEQ ID NO: 399) M78530_PEA_1_T12 1289 1304 (SEQ ID NO. 400) M78530_PEA_1_T13 1289 1304 (SEQ ID NO: 401)

Segment cluster M78530_PEA_(—)1_node_(—)18 (SEQ ID NO: 418) according to the present invention is supported by 27 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): M78530_PEA_(—)1_T11 (SEQ ID NO: 399) and M78530_PEA_(—)1_T12 (SEQ ID NO. 400). Table 28 below describes the starting and ending position of this segment on each transcript.

TABLE 28 Segment location on transcripts Segment Segment Transcript name starting position ending position M78530_PEA_1_T11 1454 1518 (SEQ ID NO: 399) M78530_PEA_1_T12 1454 1518 (SEQ ID NO. 400)

Segment cluster M78530_PEA_(—)1_node_(—)25 (SEQ ID NO: 419) according to the present invention is supported by 22 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): M78530_PEA_(—)1_T11 (SEQ ID NO: 399). Table 29 below describes the starting and ending position of this segment on each transcript.

TABLE 29 Segment location on transcripts Segment Segment Transcript name starting position ending position M78530_PEA_1_T11 1862 1937 (SEQ ID NO: 399)

Segment cluster M78530_PEA_(—)1_node_(—)30 (SEQ ID NO: 420) according to the present invention can be found in the following transcript(s): M78530_PEA_(—)1_T11 (SEQ ID NO: 399). Table 30 below describes the starting and ending position of this segment on each transcript.

TABLE 30 Segment location on transcripts Segment Segment Transcript name starting position ending position M78530_PEA_1_T11 2279 2291 (SEQ ID NO: 399)

Segment cluster M78530_PEA_(—)1_node_(—)33 (SEQ ID NO: 421) according to the present invention is supported by 32 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): M78530_PEA_(—)1_T11 (SEQ ID NO: 399). Table 31 below describes the starting and ending position of this segment on each transcript.

TABLE 31 Segment location on transcripts Segment Segment Transcript name starting position ending position M78530_PEA_1_T11 2292 2346 (SEQ ID NO: 399)

Segment cluster M78530_PEA_(—)1_node_(—)34 (SEQ ID NO: 422) according to the present invention is supported by 34 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): M78530_PEA_(—)1_T11 (SEQ ID NO: 399). Table 32 below describes the starting and ending position of this segment on each transcript.

TABLE 32 Segment location on transcripts Segment Segment Transcript name starting position ending position M78530_PEA_1_T11 2347 2459 (SEQ ID NO: 399) Variant protein alignment to the previously known protein: Sequence name: Q9HCB6 Sequence documentation:

Alignment of: M78530_PEA_(—)1_P15 (SEQ ID NO: 426) x Q9HCB6 ..

Alignment segment 1/1:

Quality: 6706.00 Escore: 0 Matching length: 665 Total length: 665 Matching Percent Similarity: 99.85 Matching Percent Identity: 99.85 Total Percent Similarity: 99.85 Total Percent Identity: 99.85 Gaps: 0

Alignment:

Sequence name: O94862 Sequence documentation:

Alignment of: M78530_PEA_(—)1_P15 (SEQ ID NO: 426) x O94862 ..

Alignment segment 1/1:

Quality: 5926.00 Escore: 0 Matching length: 582 Total length: 582 Matching Percent Similarity: 100.00 Matching Percent Identity: 100.00 Total Percent Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0

Alignment:

Sequence name: Q8NCD7 Sequence documentation:

Alignment of: M78530_PEA_(—)1_P16 (SEQ ID NO: 427) x Q8NCD7 ..

Alignment segment 1/1:

Quality: 2926.00 Escore: 0 Matching length: 297 Total length: 297 Matching Percent Similarity: 100.00 Matching Percent Identity: 100.00 Total Percent Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0

Alignment:

Sequence name: Q9HCB6 Sequence documentation:

Alignment of: M78530_PEA_(—)1_P16 (SEQ ID NO: 427) x Q9HCB6 ..

Alignment segment 1/1:

Quality: 2926.00 Escore: 0 Matching length: 297 Total length: 297 Matching Percent Similarity: 100.00 Matching Percent Identity: 100.00 Total Percent Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0

Alignment:

Sequence name: O94862 Sequence documentation:

Alignment of: M78530_PEA_(—)1_P16 (SEQ ID NO: 427) x O94862 ..

Alignment segment 1/1:

Quality: 2135.00 Escore: 0 Matching length: 214 Total length: 214 Matching Percent Similarity: 100.00 Matching Percent Identity: 100.00 Total Percent Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0

Alignment:

Sequence name: Q8NCD7 Sequence documentation:

Alignment of: M78530_PEA_(—)1_P17 (SEQ ID NO: 428) x Q8NCD7 ..

Alignment segment 1/1:

Quality: 2705.00 Escore: 0 Matching length: 275 Total length: 275 Matching Percent Similarity: 100.00 Matching Percent Identity: 100.00 Total Percent Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0

Alignment:

Sequence name: Q9HCB6 Sequence documentation:

Alignment of: M78530_PEA_(—)1_P17 (SEQ ID NO: 428) x Q9HCB6 ..

Alignment segment 1/1:

Quality: 2705.00 Escore: 0 Matching length: 275 Total length: 275 Matching Percent Similarity: 100.00 Matching Percent Identity: 100.00 Total Percent Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0

Alignment:

Sequence name: O94862 Sequence documentation:

Alignment of: M78530_PEA_(—)1_P17 (SEQ ID NO: 428) x O94862 ..

Alignment segment 1/1:

Quality: 1914.00 Escore: 0 Matching length: 192 Total length: 192 Matching Percent Similarity: 100.00 Matching Percent Identity: 100.00 Total Percent Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0

Alignment:

Description for Cluster T48119

Cluster T48119 features 1 transcript(s) and 19 segment(s) of interest, the names for which are given in Tables 1 and 2, respectively, the sequences themselves are given at the end of the application. The selected protein variants are given in table 3.

TABLE 1 Transcripts of interest Transcript Name SEQ ID NO: T48119_T2 429

TABLE 2 Segments of interest Segment Name SEQ ID NO: T48119_node_0 430 T48119_node_11 431 T48119_node_13 432 T48119_node_38 433 T48119_node_41 434 T48119_node_45 435 T48119_node_47 436 T48119_node_4 437 T48119_node_8 438 T48119_node_15 439 T48119_node_17 440 T48119_node_20 441 T48119_node_22 442 T48119_node_26 443 T48119_node_28 444 T48119_node_31 445 T48119_node_32 446 T48119_node_33 447 T48119_node_44 448

TABLE 3 Proteins of interest Protein Name SEQ ID NO: Corresponding Transcript(s) T48119_P2 450 T48119_T2 (SEQ ID NO: 429)

These sequences are variants of the known protein Programmed cell death protein 8, mitochondrial precursor (SwissProt accession identifier PCD8_HUMAN; known also according to the synonyms Apoptosis-inducing factor), SEQ ID NO: 449, referred to herein as the previously known protein.

Protein Programmed cell death protein 8, mitochondrial precursor is known or believed to have the following function(s): Probable oxidoreductase that acts as a caspase-independent mitochondrial effector of apoptotic cell death. Extramitochondrial aif induces nuclear chromatin condensation and large scale DNA fragmentation (in vitro). The sequence for protein Programmed cell death protein 8, mitochondrial precursor is given at the end of the application, as “Programmed cell death protein 8, mitochondrial precursor amino acid sequence”. Known polymorphisms for this sequence are as shown in Table 4.

TABLE 4 Amino acid mutations for Known Protein SNP position(s) on amino acid sequence Comment 36-57 GNLFQRWHVPLELQMTRQMASS -> VVQSHHLGSPSRSLA ST 62-70 GKIDNSVLV -> KDGSNLVYF 75-78 LSTV -> ATVT 82 A -> VY

Protein Programmed cell death protein 8, mitochondrial precursor localization is believed to be mitochondrial intermembrane space. Translocated to the nucleus upon induction of apoptosis.

The following GO Annotation(s) apply to the previously known protein. The following annotation(s) were found: electron transport; DNA fragmentation; apoptosis; induction of apoptosis by DNA damage, which are annotation(s) related to Biological Process; electron carrier; disulfide oxidoreductase, which are annotation(s) related to Molecular Function; and nucleus; mitochondrion, which are annotation(s) related to Cellular Component.

The GO assignment relies on information from one or more of the SwissProt/TremBl Protein knowledgebase, available from <http://www.expasy.ch/sprot/>; or Locuslink, available from <http://www.ncbi.nlm.nih.gov/projects/LocusLinkl>.

Cluster T48119 can be used as a diagnostic marker according to overexpression of transcripts of this cluster in cancer. Expression of such transcripts in normal tissues is also given according to the previously described methods. The term “number” in the left hand column of the table and the numbers on the y-axis of FIG. 41 refer to weighted expression of ESTs in each category, as “parts per million” (ratio of the expression of ESTs for a particular cluster to the expression of all ESTs in that category, according to parts per million).

Overall, the following results were obtained as shown with regard to the histograms in FIG. 41 and Table 5. This cluster is overexpressed (at least at a minimum level) in the following pathological conditions: epithelial malignant tumors and a mixture of malignant tumors from different tissues.

TABLE 5 Normal tissue distribution Name of Tissue Number adrenal 0 bladder 0 bone 0 brain 14 colon 37 epithelial 35 general 32 head and neck 10 kidney 139 liver 48 lung 15 lymph nodes 84 breast 43 bone marrow 31 ovary 0 pancreas 0 prostate 24 skin 26 stomach 0 Thyroid 128 uterus 68

TABLE 6 P values and ratios for expression in cancerous tissue Name of Tissue P1 P2 SP1 R3 SP2 R4 adrenal 4.2e−01 4.6e−01 4.6e−01 2.2 5.3e−01 1.9 bladder 1.5e−01 2.1e−01 3.2e−02 4.1 9.9e−02 2.9 bone 1 1.7e−01 1 1.0 3.4e−01 2.4 brain 7.8e−01 4.8e−01 7.1e−01 1.0 7.2e−02 2.4 colon 5.7e−01 4.5e−01 7.8e−01 1.0 3.0e−01 1.2 epithelial 2.1e−02 2.3e−03 7.3e−03 1.8 9.0e−07 2.3 general 2.4e−02 3.8e−04 6.1e−04 1.7 2.9e−11 2.2 head and neck 4.6e−01 6.2e−01 1 1.0 1 0.9 kidney 7.9e−01 8.3e−01 9.7e−01 0.4 9.9e−01 0.4 liver 3.3e−01 6.0e−01 1 1.3 6.4e−01 1.1 lung 8.5e−01 5.2e−01 3.7e−01 1.7 5.8e−02 2.5 lymph nodes 6.9e−01 5.2e−01 1 0.3 5.7e−01 0.7 breast 5.0e−01 3.9e−01 1 0.5 9.2e−01 0.7 bone marrow 8.6e−01 8.5e−01 1 0.5 7.8e−01 1.0 ovary 4.5e−02 2.0e−02 6.9e−02 3.8 7.0e−02 3.7 pancreas 1.2e−01 8.7e−02 7.6e−02 5.1 2.1e−02 5.5 prostate 8.6e−01 8.3e−01 3.6e−01 1.4 4.4e−01 1.3 skin 1.0e−01 3.1e−02 1.0e−01 4.4 2.6e−05 2.9 stomach 9.1e−01 2.1e−01 1 1.0 4.3e−02 4.0 Thyroid 4.6e−01 4.6e−01 1 0.7 1 0.7 uterus 1.2e−02 3.3e−02 7.3e−02 2.0 1.6e−01 1.6

As noted above, cluster T48119 features 1 transcript(s), which were listed in Table 1 above. These transcript(s) encode for protein(s) which are variant(s) of protein Programmed cell death protein 8, mitochondrial precursor. A description of each variant protein according to the present invention is now provided.

Variant protein T48119_P2 (SEQ ID NO: 450) according to the present invention has an amino acid sequence as given at the end of the application; it is encoded by transcript(s) T48119_T2 (SEQ ID NO: 429). An alignment is given to the known protein (Programmed cell death protein 8, mitochondrial precursor) at the end of the application. One or more alignments to one or more previously published protein sequences are given at the end of the application. A brief description of the relationship of the variant protein according to the present invention to each such aligned protein is as follows:

Comparison report between T48119_P2 (SEQ ID NO: 450) and PCD8_HUMAN:

1. An isolated chimeric polypeptide encoding for T48119_P2 (SEQ ID NO: 450), comprising a first amino acid sequence being at least 90% homologous to MTRQMASSGASGGKIDNSVLVLIVGLSTVGAGAYAYKTMKEDEKRYNERISGLGLTPE QKQKKAALSASEGEEVPQDKAPSHVPFLLIGGGTAAFAAARSIRARDPGARVLIVSEDP ELPYMRPPLSKELWFSDDPNVTKTLRFKQWNGKERSIYFQPPSFYVSAQDLPHIENGGV AVLTGKKVVQLDVRDNMVKLNDGSQITYEKCLIATGGTPRSLSAIDRAGAEVKSRTTL FRKIGDFRSLEKISREVKSITIIGGGFLGSELACALGRKARALGTEVIQLFPEKGNMGKILP EYLSNWTMEKVRREGVKVMPNAIVQSVGVSSGKLLIKLKDGRKVETDHIVAAVGLEP NVELAKTGGLEIDSDFGGFRVNAELQARSNIWVAGDAACFYDIKLGRRRVEHHDHAV VSGRLAGENMTGAAKPYWHQSMFWSDLGPDVGYEAIGLVDSSLPTVGVFAKATAQD NPKSATEQSGTGIRSESETESEASEITIPPSTPAVPQAPVQGEDYGKGVIFYLRDKVVVGI VLWNIFNRMPIARKIIKDGEQHEDLNEVAKLFNIHED corresponding to amino acids 50-613 of PCD8_HUMAN, which also corresponds to amino acids 1-564 of T48119_P2 (SEQ ID NO: 450).

The location of the variant protein was determined according to results from a number of different software programs and analyses, including analyses from SignalP and other specialized programs. The variant protein is believed to be located as follows with regard to the cell: secreted. The protein localization is believed to be secreted because one of the two signal-peptide prediction programs (HMM:Signal peptide,NN:NO) predicts that this protein has a signal peptide.

Variant protein T48119_P2 (SEQ ID NO: 450) also has the following non-silent SNPs (Single Nucleotide Polymorphisms) as listed in Table 7, (given according to their position(s) on the amino acid sequence, with the alternative amino acid(s) listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein T48119_P2 (SEQ ID NO: 450) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 7 Amino acid mutations SNP position(s) on amino acid Alternative amino Previously known sequence acid(s) SNP? 124 P -> No 124 P -> T No 308 R -> I No 507 Q -> No 545 D -> A No

Variant protein T48119_P2 (SEQ ID NO: 450) is encoded by the following transcript(s): T48119_T2 (SEQ ID NO: 429), for which the sequence(s) is/are given at the end of the application. The coding portion of transcript T48119_T2 (SEQ ID NO: 429) is shown in bold; this coding portion starts at position 227 and ends at position 1918. The transcript also has the following SNPs as listed in Table 8 (given according to their position on the nucleotide sequence, with the alternative nucleic acid listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein T48119_P2 (SEQ ID NO: 450) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 8 Nucleic acid SNPs SNP position on nucleotide Alternative Previously sequence nucleic acid known SNP? 352 C -> T Yes 596 C -> No 596 C -> A No 766 -> G No 997 C -> T Yes 1075 A -> G Yes 1149 G -> T No 1747 G -> No 1786 G -> A Yes 1860 A -> C No

As noted above, cluster T48119 features 19 segment(s), which were listed in Table 2 above and for which the sequence(s) are given at the end of the application. These segment(s) are portions of nucleic acid sequence(s) which are described herein separately because they are of particular interest. A description of each segment according to the present invention is now provided.

Segment cluster T48119_node_(—)0 (SEQ ID NO: 430) according to the present invention is supported by 81 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): T48119_T2 (SEQ ID NO: 429). Table 9 below describes the starting and ending position of this segment on each transcript.

TABLE 9 Segment location on transcripts Segment Segment Transcript name starting position ending position T48119_T2 1 226 (SEQ ID NO: 429)

Segment cluster T48119_node_(—)11 (SEQ ID NO: 431) according to the present invention is supported by 77 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): T48119_T2 (SEQ ID NO: 429). Table 10 below describes the starting and ending position of this segment on each transcript.

TABLE 10 Segment location on transcripts Segment Segment Transcript name starting position ending position T48119_T2 429 553 (SEQ ID NO: 429)

Segment cluster T48119_node_(—)13 (SEQ ID NO: 432) according to the present invention is supported by 74 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): T48119_T2 (SEQ ID NO: 429). Table 11 below describes the starting and ending position of this segment on each transcript.

TABLE 11 Segment location on transcripts Segment Segment Transcript name starting position ending position T48119_T2 554 684 (SEQ ID NO: 429)

Segment cluster T48119_node_(—)38 (SEQ ID NO: 433) according to the present invention is supported by 119 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): T48119_T2 (SEQ ID NO: 429). Table 12 below describes the starting and ending position of this segment on each transcript.

TABLE 12 Segment location on transcripts Segment Segment Transcript name starting position ending position T48119_T2 1385 1527 (SEQ ID NO: 429)

Segment cluster T48119_node_(—)41 (SEQ ID NO: 434) according to the present invention is supported by 128 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): T48119_T2 (SEQ ID NO: 429). Table 13 below describes the starting and ending position of this segment on each transcript.

TABLE 13 Segment location on transcripts Segment Segment Transcript name starting position ending position T48119_T2 1528 1652 (SEQ ID NO: 429)

Microarray (chip) data is also available for this segment as follows. As described above with regard to the cluster itself, various oligonucleotides were tested for being differentially expressed in various disease conditions, particularly cancer. The following oligonucleotides were found to hit this segment (in regard to ovarian cancer), shown in Table 14.

TABLE 14 Oligonucleotides related to this segment Overexpressed Oligonucleotide name in cancers Chip reference T99761_0_13_0 ovarian carcinoma OVA

Segment cluster T48119_node_(—)45 (SEQ ID NO: 435) according to the present invention is supported by 138 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): T48119_T2 (SEQ ID NO: 429). Table 15 below describes the starting and ending position of this segment on each transcript.

TABLE 15 Segment location on transcripts Segment Segment Transcript name starting position ending position T48119_T2 1717 1849 (SEQ ID NO: 429)

Segment cluster T48119_node_(—)47 (SEQ ID NO: 436) according to the present invention is supported by 129 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): T48119_T2 (SEQ ID NO: 429). Table 16 below describes the starting and ending position of this segment on each transcript.

TABLE 16 Segment location on transcripts Segment Segment Transcript name starting position ending position 148119_T2 1850 2113 (SEQ ID NO: 429)

According to an optional embodiment of the present invention, short segments related to the above cluster are also provided. These segments are up to about 120 bp in length, and so are included in a separate description.

Segment cluster T48119_node_(—)4 (SEQ ID NO: 437) according to the present invention is supported by 81 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): T48119_T2 (SEQ ID NO: 429). Table 17 below describes the starting and ending position of this segment on each transcript.

TABLE 17 Segment location on transcripts Segment Segment Transcript starting ending name position position T48119_T2 227 328 (SEQ ID NO:429)

Segment cluster T48119_node_(—)8 (SEQ ID NO: 438) according to the present invention is supported by 79 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): T48119_T2 (SEQ ID NO: 429). Table 18 below describes the starting and ending position of this segment on each transcript.

TABLE 18 Segment location on transcripts Segment Segment Transcript starting ending name position position T48119_T2 329 428 (SEQ ID NO:429)

Segment cluster T48119_node_(—)15 (SEQ ID NO: 439) according to the present invention is supported by 64 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): T48119_T2 (SEQ ID NO: 429). Table 19 below describes the starting and ending position of this segment on each transcript.

TABLE 19 Segment location on transcripts Segment Segment Transcript starting ending name position position T48119_T2 685 775 (SEQ ID NO:429)

Segment cluster T48119_node_(—)17 (SEQ ID NO: 440) according to the present invention is supported by 59 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): T48119_T2 (SEQ ID NO: 429). Table 20 below describes the starting and ending position of this segment on each transcript.

TABLE 20 Segment location on transcripts Segment Segment Transcript starting ending name position position T48119_T2 776 860 (SEQ ID NO:429)

Segment cluster T48119_node_(—)20 (SEQ ID NO: 441) according to the present invention is supported by 64 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): T48119_T2 (SEQ ID NO: 429). Table 21 below describes the starting and ending position of this segment on each transcript.

TABLE 21 Segment location on transcripts Segment Segment Transcript starting ending name position position T48119_T2 861 937 (SEQ ID NO:429)

Segment cluster T48119_node_(—)22 (SEQ ID NO: 442) according to the present invention is supported by 73 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): T48119_T2 (SEQ ID NO: 429). Table 22 below describes the starting and ending position of this segment on each transcript.

TABLE 22 Segment location on transcripts Segment Segment Transcript starting ending name position position T48119_T2 938 1046 (SEQ ID NO:429)

Segment cluster T48119_node_(—)26 (SEQ ID NO: 443) according to the present invention is supported by 86 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): T48119_T2 (SEQ ID NO: 429). Table 23 below describes the starting and ending position of this segment on each transcript.

TABLE 23 Segment location on transcripts Segment Segment Transcript starting ending name position position T48119_T2 1047 1154 (SEQ ID NO:429)

Segment cluster T48119_node_(—)28 (SEQ ID NO: 444) according to the present invention is supported by 83 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): T48119_T2 (SEQ ID NO: 429). Table 24 below describes the starting and ending position of this segment on each transcript.

TABLE 24 Segment location on transcripts Segment Segment Transcript starting ending name position position T48119_T2 1155 1243 (SEQ ID NO:429)

Segment cluster T48119_node_(—)31 (SEQ ID NO: 445) according to the present invention is supported by 83 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): T48119_T2 (SEQ ID NO: 429). Table 25 below describes the starting and ending position of this segment on each transcript.

TABLE 25 Segment location on transcripts Segment Segment Transcript starting ending name position position T48119_T2 1244 1304 (SEQ ID NO:429)

Segment cluster T48119_node_(—)32 (SEQ ID NO: 446) according to the present invention can be found in the following transcript(s): T48119_T2 (SEQ ID NO: 429). Table 26 below describes the starting and ending position of this segment on each transcript.

TABLE 26 Segment location on transcripts Segment Segment Transcript starting ending name position position T48119_T2 1305 1328 (SEQ ID NO:429)

Segment cluster T48119_node_(—)33 (SEQ ID NO: 447) according to the present invention is supported by 89 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): T48119_T2 (SEQ ID NO: 429). Table 27 below describes the starting and ending position of this segment on each transcript.

TABLE 27 Segment location on transcripts Segment Segment Transcript starting ending name position position T48119_T2 1329 1384 (SEQ ID NO:429)

Segment cluster T48119_node_(—)44 (SEQ ID NO: 448) according to the present invention is supported by 140 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): T48119_T2 (SEQ ID NO: 429). Table 28 below describes the starting and ending position of this segment on each transcript.

TABLE 28 Segment location on transcripts Segment Segment Transcript starting ending name position position T48119_T2 1653 1716 (SEQ ID NO:429) Variant protein alignment to the previously known protein: Sequence name: PCD8_HUMAN Sequence documentation:

Alignment of: T48119_P2 (SEQ ID NO: 450) x PCD8_HUMAN ..

Alignment segment 1/1:

Quality: 5416.00 Escore: 0 Matching length: 564 Total length: 564 Matching Percent Similarity: 100.00 Matching Percent Identity: 100.00 Total Percent Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0

Alignment:

Description for Cluster HSMUC1A

Cluster HSMUC1A features 14 transcript(s) and 22 segment(s) of interest, the names for which are given in Tables 1 and 2, respectively, the sequences themselves are given at the end of the application. The selected protein variants are given in table 3.

TABLE 1 Transcripts of interest Transcript Name SEQ ID NO: HSMUC1A_PEA_1_T12 451 HSMUC1A_PEA_1_T26 452 HSMUC1A_PEA_1_T28 453 HSMUC1A_PEA_1_T29 454 HSMUC1A_PEA_1_T30 455 HSMUC1A_PEA_1_T31 456 HSMUC1A_PEA_1_T33 457 HSMUC1A_PEA_1_T34 458 HSMUC1A_PEA_1_T35 459 HSMUC1A_PEA_1_T36 460 HSMUC1A_PEA_1_T40 461 HSMUC1A_PEA_1_T42 462 HSMUC1A_PEA_1_T43 463 HSMUC1A_PEA_1_T47 464

TABLE 2 Segments of interest Segment Name SEQ ID NO: HSMUC1A_PEA_1_node_0 465 HSMUC1A_PEA_1_node_14 466 HSMUC1A_PEA_1_node_24 467 HSMUC1A_PEA_1_node_29 468 HSMUC1A_PEA_1_node_35 469 HSMUC1A_PEA_1_node_38 470 HSMUC1A_PEA_1_node_3 471 HSMUC1A_PEA_1_node_4 472 HSMUC1A_PEA_1_node_5 473 HSMUC1A_PEA_1_node_6 474 HSMUC1A_PEA_1_node_7 475 HSMUC1A_PEA_1_node_17 476 HSMUC1A_PEA_1_node_18 477 HSMUC1A_PEA_1_node_20 478 HSMUC1A_PEA_1_node_21 479 HSMUC1A_PEA_1_node_23 480 HSMUC1A_PEA_1_node_26 481 HSMUC1A_PEA_1_node_27 482 HSMUC1A_PEA_1_node_31 483 HSMUC1A_PEA_1_node_34 484 HSMUC1A_PEA_1_node_36 485 HSMUC1A_PEA_1_node_37 486

TABLE 3 Proteins of interest Corresponding Protein Name SEQ ID NO: Transcript(s) HSMUC1A_PEA_1_P25 488 HSMUC1A_PEA_1_T26 (SEQ ID NO:452) HSMUC1A_PEA_1_P29 489 HSMUC1A_PEA_1_T33 (SEQ ID NO:457) HSMUC1A_PEA_1_P30 490 HSMUC1A_PEA_1_T34 (SEQ ID NO:458) HSMUC1A_PEA_1_P32 491 HSMUC1A_PEA_1_T36 (SEQ ID NO:460) HSMUC1A_PEA_1_P36 492 HSMUC1A_PEA_1_T40 (SEQ ID NO:461) HSMUC1A_PEA_1_P39 493 HSMUC1A_PEA_1_T43 (SEQ ID NO:463) HSMUC1A_PEA_1_P45 494 HSMUC1A_PEA_1_T29 (SEQ ID NO:454) HSMUC1A_PEA_1_P49 495 HSMUC1A_PEA_1_T12 (SEQ ID NO:451) HSMUC1A_PEA_1_P52 496 HSMUC1A_PEA_1_T30 (SEQ ID NO:455) HSMUC1A_PEA_1_P53 497 HSMUC1A_PEA_1_T31 (SEQ ID NO:456) HSMUC1A_PEA_1_P56 498 HSMUC1A_PEA_1_T42 (SEQ ID NO:462) HSMUC1A_PEA_1_P58 499 HSMUC1A_PEA_1_T35 (SEQ ID NO:459) HSMUC1A_PEA_1_P59 500 HSMUC1A_PEA_1_T28 (SEQ ID NO:453) HSMUC1A_PEA_1_P63 501 HSMUC1A_PEA_1_T47 (SEQ ID NO:464)

These sequences are variants of the known protein Mucin 1 precursor (SwissProt accession identifier MUC1_HUMAN; known also according to the synonyms MUC-1; Polymorphic epithelial mucin; PEM; PEMT; Episialin; Tumor-associated mucin; Carcinoma-associated mucin; Tumor-associated epithelial membrane antigen; EMA; H23AG; Peanut-reactive urinary mucin; PUM; Breast carcinoma-associated antigen DF3; CD227 antigen), SEQ ID NO: 487, referred to herein as the previously known protein.

Protein Mucin 1 precursor is known or believed to have the following function(s): May play a role in adhesive functions and in cell-cell interactions, metastasis and signaling. May provide a protective layer on epithelial surfaces. Direct or indirect interaction with actin cytoskeleton. Isoform 7 behaves as a receptor and binds the secreted isoform 5. The binding induces the phosphorylation of the isoform 7, alters cellular morphology and initiates cell signaling. Can bind to GRB2 adapter protein. The sequence for protein Mucin 1 precursor is given at the end of the application, as “Mucin 1 precursor amino acid sequence”. Known polymorphisms for this sequence are as shown in Table 4.

TABLE 4 Amino acid mutations for Known Protein SNP position(s) on amino acid sequence Comment 1116 D->E: NO EFFECT ON BINDING OF ISOFORM 7. 1116 D->A: DRASTICALLY REDUCED BINDING OF ISOFORM 7. 2 T -> A 134 P -> Q 154 P -> Q 1021 S -> T 1117 V -> M 1193 Q -> L 1231 K -> T 1251 A -> T

Protein Mucin 1 precursor localization is believed to be Type I membrane protein. Two secreted forms (5 and 9) are also produced.

The previously known protein also has the following indication(s) and/or potential therapeutic use(s): Cancer, breast; Cancer, lung, non-small cell; Cancer, ovarian; Cancer, prostate; Cancer. It has been investigated for clinical/therapeutic use in humans, for example as a target for an antibody or small molecule, and/or as a direct therapeutic; available information related to these investigations is as follows. Potential pharmaceutically related or therapeutically related activity or activities of the previously known protein are as follows: CD8 agonist; DNA antagonist; Immunostimulant; Interferon gamma agonist; MUC-1 inhibitor. A therapeutic role for a protein represented by the cluster has been predicted. The cluster was assigned this field because there was information in the drug database or the public databases (e.g., described herein above) that this protein, or part thereof, is used or can be used for a potential therapeutic indication: Anticancer; Monoclonal antibody, murine; Immunotoxin; Immunostimulant; Immunoconjugate.

The following GO Annotation(s) apply to the previously known protein. The following annotation(s) were found: actin binding, which are annotation(s) related to Molecular Function; and cytoskeleton; integral plasma membrane protein, which are annotation(s) related to Cellular Component.

The GO assignment relies on information from one or more of the SwissProt/TremBl Protein knowledgebase, available from <http://www.expasy.ch/sprot/>; or Locuslink, available from <http://www.ncbi.nlm.nih.gov/projects/LocusLink/>.

Cluster HSMUC1A can be used as a diagnostic marker according to overexpression of transcripts of this cluster in cancer. Expression of such transcripts in normal tissues is also given according to the previously described methods. The term “number” in the left hand column of the table and the numbers on the y-axis of FIG. 42 refer to weighted expression of ESTs in each category, as “parts per million” (ratio of the expression of ESTs for a particular cluster to the expression of all ESTs in that category, according to parts per million).

Overall, the following results were obtained as shown with regard to the histograms in FIG. 42 and Table 5. This cluster is overexpressed (at least at a minimum level) in the following pathological conditions: a mixture of malignant tumors from different tissues, breast malignant tumors, pancreas carcinoma and prostate cancer.

TABLE 5 Normal tissue distribution Name of Tissue Number bladder 41 brain 2 colon 66 epithelial 96 general 36 head and neck 314 kidney 282 lung 200 breast 61 ovary 0 pancreas 12 prostate 24 stomach 296 Thyroid 0 uterus 122

TABLE 6 P values and ratios for expression in cancerous tissue Name of Tissue P1 P2 SP1 R3 SP2 R4 bladder 3.3e−01 4.5e−01 1.8e−02 2.4 8.9e−02 1.7 brain 3.0e−02 2.6e−02 1.2e−01 4.6 1.1e−01 3.9 colon 1.2e−01 2.4e−01 3.8e−01 1.6 5.9e−01 1.2 epithelial 5.4e−02 6.0e−01 7.3e−06 1.8 6.2e−02 1.1 general 6.5e−07 2.6e−03 4.0e−23 3.6 1.7e−12 2.3 head and neck 6.4e−01 7.2e−01 1 0.3 1 0.3 kidney 7.8e−01 8.1e−01 1 0.3 1 0.2 lung 7.6e−01 7.9e−01 6.7e−01 0.8 1 0.4 breast 8.2e−02 1.3e−01 4.1e−03 3.6 7.7e−02 2.0 ovary 3.0e−02 4.3e−02 6.9e−02 4.4 1.6e−01 3.2 pancreas 7.2e−02 1.4e−01 9.6e−07 5.4 1.5e−05 4.5 prostate 7.0e−01 6.0e−01 1.5e−02 1.4 6.9e−04 3.2 stomach 3.1e−01 7.1e−01 1.5e−01 0.4 4.6e−01 0.8 Thyroid 2.9e−01 2.9e−01 4.4e−01 2.0 4.4e−01 2.0 uterus 2.4e−01 6.5e−01 1.6e−01 1.0 7.0e−01 0.6

For this cluster, at least one oligonucleotide was found to demonstrate overexpression of the cluster, although not of at least one transcript/segment as listed below. Microarray (chip) data is also available for this cluster as follows. Various oligonucleotides were tested for being differentially expressed in various disease conditions, particularly cancer, as previously described. The following oligonucleotides were found to hit this cluster but not other segments/transcripts below (in regard to ovarian cancer), shown in Table 7.

TABLE 7 Oligonucleotides related to this cluster Overexpressed Chip Oligonucleotide name in cancers reference HSMUC1A_0_0_11364 ovarian carcinoma OVA

As noted above, cluster HSMUC1A features 14 transcript(s), which were listed in Table 1 above. These transcript(s) encode for protein(s) which are variant(s) of protein Mucin 1 precursor. A description of each variant protein according to the present invention is now provided.

Variant protein HSMUC1A_PEA_(—)1_P25 (SEQ ID NO: 488) according to the present invention has an amino acid sequence as given at the end of the application; it is encoded by transcript(s) HSMUC1A_PEA_(—)1_T26 (SEQ ID NO: 452). The location of the variant protein was determined according to results from a number of different software programs and analyses, including analyses from SignalP and other specialized programs. The variant protein is believed to be located as follows with regard to the cell: secreted. The protein localization is believed to be secreted because both signal-peptide prediction programs predict that this protein has a signal peptide.

Variant protein HSMUC1A_PEA_(—)1_P25 (SEQ ID NO: 488) also has the following non-silent SNPs (Single Nucleotide Polymorphisms) as listed in Table 8, (given according to their position(s) on the amino acid sequence, with the alternative amino acid(s) listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein HSMUC1A_PEA_(—)1_P25 (SEQ ID NO: 488) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 8 Amino acid mutations SNP position(s) on amino acid Alternative Previously sequence amino acid(s) known SNP? 90 S -> N Yes 91 D -> N No 157 Y -> No 187 S -> G No

Variant protein HSMUC1A_PEA_(—)1_P25 (SEQ ID NO: 488) is encoded by the following transcript(s): HSMUC1A_PEA_(—)1_T26 (SEQ ID NO: 452), for which the sequence(s) is/are given at the end of the application. The coding portion of transcript HSMUC1A_PEA_(—)1_T26 (SEQ ID NO: 452) is shown in bold; this coding portion starts at position 507 and ends at position 1115. The transcript also has the following SNPs as listed in Table 9 (given according to their position on the nucleotide sequence, with the alternative nucleic acid listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein HSMUC1A_PEA_(—)1_P25 (SEQ ID NO: 488) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 9 Nucleic acid SNPs SNP position on nucleotide Alternative Previously sequence nucleic acid known SNP? 572 A -> G No 775 G -> A Yes 777 G -> A No 977 C -> No 1065 A -> G No 1073 C -> T No 1079 C -> T Yes 1124 C -> T Yes 1177 C -> T No 1197 C -> T Yes 1303 G -> No 1315 G -> A Yes 1316 C -> No 1316 C -> T No 1405 A -> T No

Variant protein HSMUC1A_PEA_(—)1_P29 (SEQ ID NO: 489) according to the present invention has an amino acid sequence as given at the end of the application; it is encoded by transcript(s) HSMUC1A_PEA_(—)1_T33 (SEQ ID NO: 457). The location of the variant protein was determined according to results from a number of different software programs and analyses, including analyses from SignalP and other specialized programs. The variant protein is believed to be located as follows with regard to the cell: secreted. The protein localization is believed to be secreted because both signal-peptide prediction programs predict that this protein has a signal peptide, and neither trans-membrane region prediction program predicts that this protein has a trans-membrane region.

Variant protein HSMUC1A_PEA_(—)1_P29 (SEQ ID NO: 489) is encoded by the following transcript(s): HSMUC1A_PEA_(—)1_T33 (SEQ ID NO: 457), for which the sequence(s) is/are given at the end of the application. The coding portion of transcript HSMUC1A_PEA_(—)1_T33 (SEQ ID NO: 457) is shown in bold; this coding portion starts at position 507 and ends at position 953. The transcript also has the following SNPs as listed in Table 10 (given according to their position on the nucleotide sequence, with the alternative nucleic acid listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein HSMUC1A_PEA_(—)1_P29 (SEQ ID NO: 489) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 10 Nucleic acid SNPs SNP position on nucleotide Alternative Previously sequence nucleic acid known SNP? 572 A -> G No 964 C -> No 1052 A -> G No 1060 C -> T No 1066 C -> T Yes 1111 C -> T Yes 1164 C -> T No 1184 C -> T Yes 1290 G -> No 1302 G -> A Yes 1303 C -> No 1303 C -> T No 1392 A -> T No

Variant protein HSMUC1A_PEA_(—)1_P30 (SEQ ID NO: 490) according to the present invention has an amino acid sequence as given at the end of the application; it is encoded by transcript(s) HSMUC1A_PEA_(—)1_T34 (SEQ ID NO: 458). The location of the variant protein was determined according to results from a number of different software programs and analyses, including analyses from SignalP and other specialized programs. The variant protein is believed to be located as follows with regard to the cell: secreted. The protein localization is believed to be secreted because both signal-peptide prediction programs predict that this protein has a signal peptide.

Variant protein HSMUC1A_PEA_(—)1_P30 (SEQ ID NO: 490) also has the following non-silent SNPs (Single Nucleotide Polymorphisms) as listed in Table 11, (given according to their position(s) on the amino acid sequence, with the alternative amino acid(s) listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein HSMUC1A_PEA_(—)1_P30 (SEQ ID NO: 490) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 11 Amino acid mutations SNP position(s) on amino acid Alternative Previously sequence amino acid(s) known SNP? 120 Y -> No 150 S -> G No

Variant protein HSMUC1A_PEA_(—)1_P30 (SEQ ID NO: 490) is encoded by the following transcript(s): HSMUC1A_PEA_(—)1_T34 (SEQ ID NO: 458), for which the sequence(s) is/are given at the end of the application. The coding portion of transcript HSMUC1A_PEA_(—)1_T34 (SEQ ID NO: 458) is shown in bold; this coding portion starts at position 507 and ends at position 1004. The transcript also has the following SNPs as listed in Table 12 (given according to their position on the nucleotide sequence, with the alternative nucleic acid listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein HSMUC1A_PEA_(—)1_P30 (SEQ ID NO: 490) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 12 Nucleic acid SNPs SNP position on nucleotide Alternative Previously sequence nucleic acid known SNP? 599 A -> G No 866 C -> No 954 A -> G No 962 C -> T No 968 C -> T Yes 1013 C -> T Yes 1066 C -> T No 1086 C -> T Yes 1192 G -> No 1204 G -> A Yes 1205 C -> No 1205 C -> T No 1294 A -> T No

Variant protein HSMUC1A_PEA_(—)1_P32 (SEQ ID NO: 491) according to the present invention has an amino acid sequence as given at the end of the application; it is encoded by transcript(s) HSMUC1A_PEA_(—)1_T36 (SEQ ID NO: 460). The location of the variant protein was determined according to results from a number of different software programs and analyses, including analyses from SignalP and other specialized programs. The variant protein is believed to be located as follows with regard to the cell: secreted. The protein localization is believed to be secreted because both signal-peptide prediction programs predict that this protein has a signal peptide.

Variant protein HSMUC1A_PEA_(—)1_P32 (SEQ ID NO: 491) also has the following non-silent SNPs (Single Nucleotide Polymorphisms) as listed in Table 13, (given according to their position(s) on the amino acid sequence, with the alternative amino acid(s) listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein HSMUC1A_PEA_(—)1_P32 (SEQ ID NO: 491) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 13 Amino acid mutations SNP position(s) on amino acid Alternative Previously sequence amino acid(s) known SNP? 111 Y -> No 141 S -> G No

Variant protein HSMUC1A_PEA_(—)1_P32 (SEQ ID NO: 491) is encoded by the following transcript(s): HSMUC1A_PEA_(—)1_T36 (SEQ ID NO: 460), for which the sequence(s) is/are given at the end of the application. The coding portion of transcript HSMUC1A_PEA_(—)1_T36 (SEQ ID NO: 460) is shown in bold; this coding portion starts at position 507 and ends at position 977. The transcript also has the following SNPs as listed in Table 14 (given according to their position on the nucleotide sequence, with the alternative nucleic acid listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein HSMUC1A_PEA_(—)1_P32 (SEQ ID NO: 491) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 14 Nucleic acid SNPs SNP position on nucleotide Alternative Previously sequence nucleic acid known SNP? 572 A -> G No 839 C -> No 927 A -> G No 935 C -> T No 941 C -> T Yes 986 C -> T Yes 1039 C -> T No 1059 C -> T Yes 1165 G -> No 1177 G -> A Yes 1178 C -> No 1178 C -> T No 1267 A -> T No

Variant protein HSMUC1A_PEA_(—)1_P36 (SEQ ID NO: 492) according to the present invention has an amino acid sequence as given at the end of the application; it is encoded by transcript(s) HSMUC1A_PEA_(—)1_T40 (SEQ ID NO: 461). The location of the variant protein was determined according to results from a number of different software programs and analyses, including analyses from SignalP and other specialized programs. The variant protein is believed to be located as follows with regard to the cell: secreted. The protein localization is believed to be secreted because both signal-peptide prediction programs predict that this protein has a signal peptide, and neither trans-membrane region prediction program predicts that this protein has a trans-membrane region.

Variant protein HSMUC1A_PEA_(—)1_P36 (SEQ ID NO: 492) also has the following non-silent SNPs (Single Nucleotide Polymorphisms) as listed in Table 15, (given according to their position(s) on the amino acid sequence, with the alternative amino acid(s) listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein HSMUC1A_PEA_(—)1_P36 (SEQ ID NO: 492) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 15 Amino acid mutations SNP position(s) on amino acid Previously sequence Alternative amino acid(s) known SNP? 113 Y -> No 143 S -> G No

Variant protein HSMUC1A_PEA_(—)1_P36 (SEQ ID NO: 492) is encoded by the following transcript(s): HSMUC1A_PEA_(—)1_T40 (SEQ ID NO: 461), for which the sequence(s) is/are given at the end of the application. The coding portion of transcript HSMUC1A_PEA_(—)1_T40 (SEQ ID NO: 461) is shown in bold; this coding portion starts at position 507 and ends at position 983. The transcript also has the following SNPs as listed in Table 16 (given according to their position on the nucleotide sequence, with the alternative nucleic acid listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein HSMUC1A_PEA_(—)1_P36 (SEQ ID NO: 492) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 16 Nucleic acid SNPs SNP position on nucleotide Alternative Previously sequence nucleic acid known SNP? 599 A -> G No 845 C -> No 933 A -> G No 941 C -> T No 947 C -> T Yes 992 C -> T Yes 1045 C -> T No 1065 C -> T Yes 1171 G -> No 1183 G -> A Yes 1184 C -> No 1184 C -> T No 1273 A -> T No

Variant protein HSMUC1A_PEA_(—)1_P39 (SEQ ID NO: 493) according to the present invention has an amino acid sequence as given at the end of the application; it is encoded by transcript(s) HSMUC1A_PEA_(—)1_T43 (SEQ ID NO: 463). The location of the variant protein was determined according to results from a number of different software programs and analyses, including analyses from SignalP and other specialized programs. The variant protein is believed to be located as follows with regard to the cell: secreted. The protein localization is believed to be secreted because both signal-peptide prediction programs predict that this protein has a signal peptide, and neither trans-membrane region prediction program predicts that this protein has a trans-membrane region.

Variant protein HSMUC1A_PEA_(—)1_P39 (SEQ ID NO: 493) also has the following non-silent SNPs (Single Nucleotide Polymorphisms) as listed in Table 17, (given according to their position(s) on the amino acid sequence, with the alternative amino acid(s) listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein HSMUC1A_PEA_(—)1_P39 (SEQ ID NO: 493) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 17 Amino acid mutations SNP position(s) on amino acid Alternative Previously sequence amino acid(s) known SNP? 90 Y -> No 120 S -> G No

Variant protein HSMUC1A_PEA_(—)1_P39 (SEQ ID NO: 493) is encoded by the following transcript(s): HSMUC1A_PEA_(—)1_T43 (SEQ ID NO: 463), for which the sequence(s) is/are given at the end of the application. The coding portion of transcript HSMUC1A_PEA_(—)1_T43 (SEQ ID NO: 463) is shown in bold; this coding portion starts at position 507 and ends at position 914. The transcript also has the following SNPs as listed in Table 18 (given according to their position on the nucleotide sequence, with the alternative nucleic acid listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein HSMUC1A_PEA_(—)1_P39 (SEQ ID NO: 493) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 18 Nucleic acid SNPs SNP position on nucleotide Alternative Previously sequence nucleic acid known SNP? 599 A -> G No 776 C -> No 864 A -> G No 872 C -> T No 878 C -> T Yes 923 C -> T Yes 976 C -> T No 996 C -> T Yes 1102 G -> No 1114 G -> A Yes 1115 C -> No 1115 C -> T No 1204 A -> T No

Variant protein HSMUC1A_PEA_(—)1_P45 (SEQ ID NO: 494) according to the present invention has an amino acid sequence as given at the end of the application; it is encoded by transcript(s) HSMUC1A_PEA_(—)1_T29 (SEQ ID NO: 454). The location of the variant protein was determined according to results from a number of different software programs and analyses, including analyses from SignalP and other specialized programs. The variant protein is believed to be located as follows with regard to the cell: secreted. The protein localization is believed to be secreted because both signal-peptide prediction programs predict that this protein has a signal peptide, and neither trans-membrane region prediction program predicts that this protein has a trans-membrane region.

Variant protein HSMUC1A_PEA_(—)1_P45 (SEQ ID NO: 494) is encoded by the following transcript(s): HSMUC1A_PEA_(—)1_T29 (SEQ ID NO: 454), for which the sequence(s) is/are given at the end of the application. The coding portion of transcript HSMUC1A_PEA_(—)1_T29 (SEQ ID NO: 454) is shown in bold; this coding portion starts at position 507 and ends at position 746. The transcript also has the following SNPs as listed in Table 19 (given according to their position on the nucleotide sequence, with the alternative nucleic acid listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein HSMUC1A_PEA_(—)1_P45 (SEQ ID NO: 494) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 19 Nucleic acid SNPs SNP position on nucleotide Alternative Previously sequence nucleic acid known SNP? 599 A -> G No 746 G -> A Yes 748 G -> A No 948 C -> No 1036 A -> G No 1044 C -> T No 1050 C -> T Yes 1095 C -> T Yes 1148 C -> T No 1168 C -> T Yes 1274 G -> No 1286 G -> A Yes 1287 C -> No 1287 C -> T No 1376 A -> T No

Variant protein HSMUC1A_PEA_(—)1_P49 (SEQ ID NO: 495) according to the present invention has an amino acid sequence as given at the end of the application; it is encoded by transcript(s) HSMUC1A_PEA_(—)1_T12 (SEQ ID NO: 451). The location of the variant protein was determined according to results from a number of different software programs and analyses, including analyses from SignalP and other specialized programs. The variant protein is believed to be located as follows with regard to the cell: secreted. The protein localization is believed to be secreted because both signal-peptide prediction programs predict that this protein has a signal peptide, and neither trans-membrane region prediction program predicts that this protein has a trans-membrane region.

Variant protein HSMUC1A_PEA_(—)1_P49 (SEQ ID NO: 495) is encoded by the following transcript(s): HSMUC1A_PEA_(—)1_T12 (SEQ ID NO: 451), for which the sequence(s) is/are given at the end of the application. The coding portion of transcript HSMUC1A_PEA_(—)1_T12 (SEQ ID NO: 451) is shown in bold; this coding portion starts at position 507 and ends at position 884. The transcript also has the following SNPs as listed in Table 20 (given according to their position on the nucleotide sequence, with the alternative nucleic acid listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein HSMUC1A_PEA_(—)1_P49 (SEQ ID NO: 495) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 20 Nucleic acid SNPs SNP position on nucleotide Alternative Previously sequence nucleic acid known SNP? 572 A -> G No 704 G -> A Yes 1012 G -> A Yes 1088 G -> A Yes 1090 G -> A No 1290 C -> No 1378 A -> G No 1386 C -> T No 1392 C -> T Yes 1437 C -> T Yes 1490 C -> T No 1510 C -> T Yes 1616 G -> No 1628 G -> A Yes 1629 C -> No 1629 C -> T No 1718 A -> T No

Variant protein HSMUC1A_PEA_(—)1_P52 (SEQ ID NO: 496) according to the present invention has an amino acid sequence as given at the end of the application; it is encoded by transcript(s) HSMUC1A_PEA_(—)1_T30 (SEQ ID NO: 455). The location of the variant protein was determined according to results from a number of different software programs and analyses, including analyses from SignalP and other specialized programs. The variant protein is believed to be located as follows with regard to the cell: secreted. The protein localization is believed to be secreted because both signal-peptide prediction programs predict that this protein has a signal peptide, and neither trans-membrane region prediction program predicts that this protein has a trans-membrane region.

Variant protein HSMUC1A_PEA_(—)1_P52 (SEQ ID NO: 496) is encoded by the following transcript(s): HSMUC1A_PEA_(—)1_T30 (SEQ ID NO: 455), for which the sequence(s) is/are given at the end of the application. The coding portion of transcript HSMUC1A_PEA_(—)1_T30 (SEQ ID NO: 455) is shown in bold; this coding portion starts at position 507 and ends at position 719. The transcript also has the following SNPs as listed in Table 21 (given according to their position on the nucleotide sequence, with the alternative nucleic acid listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein HSMUC1A_PEA_(—)1_P52 (SEQ ID NO: 496) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 21 Nucleic acid SNPs SNP position on nucleotide Alternative Previously sequence nucleic acid known SNP? 572 A -> G No 719 G -> A Yes 721 G -> A No 921 C -> No 1009 A -> G No 1017 C -> T No 1023 C -> T Yes 1068 C -> T Yes 1121 C -> T No 1141 C -> T Yes 1247 G -> No 1259 G -> A Yes 1260 C -> No 1260 C -> T No 1349 A -> T No

Variant protein HSMUC1A_PEA_(—)1_P53 (SEQ ID NO: 497) according to the present invention has an amino acid sequence as given at the end of the application; it is encoded by transcript(s) HSMUC1A_PEA_(—)1_T31 (SEQ ID NO: 456). The location of the variant protein was determined according to results from a number of different software programs and analyses, including analyses from SignalP and other specialized programs. The variant protein is believed to be located as follows with regard to the cell: secreted. The protein localization is believed to be secreted because both signal-peptide prediction programs predict that this protein has a signal peptide, and neither trans-membrane region prediction program predicts that this protein has a trans-membrane region.

Variant protein HSMUC1A_PEA_(—)1_P53 (SEQ ID NO: 497) is encoded by the following transcript(s): HSMUC1A_PEA_(—)1_T31 (SEQ ID NO: 456), for which the sequence(s) is/are given at the end of the application. The coding portion of transcript HSMUC1A_PEA_(—)1_T31 (SEQ ID NO: 456) is shown in bold; this coding portion starts at position 507 and ends at position 665. The transcript also has the following SNPs as listed in Table 22 (given according to their position on the nucleotide sequence, with the alternative nucleic acid listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein HSMUC1A_PEA_(—)1_P53 (SEQ ID NO: 497) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 22 Nucleic acid SNPs SNP position on nucleotide Alternative Previously sequence nucleic acid known SNP? 572 A -> G No 669 G -> A Yes 671 G -> A No 871 C -> No 959 A -> G No 967 C -> T No 973 C -> T Yes 1018 C -> T Yes 1071 C -> T No 1091 C -> T Yes 1197 G -> No 1209 G -> A Yes 1210 C -> No 1210 C -> T No 1299 A -> T No

Variant protein HSMUC1A_PEA_(—)1_P56 (SEQ ID NO: 498) according to the present invention has an amino acid sequence as given at the end of the application; it is encoded by transcript(s) HSMUC1A_PEA_(—)1_T42 (SEQ ID NO: 462). The location of the variant protein was determined according to results from a number of different software programs and analyses, including analyses from SignalP and other specialized programs. The variant protein is believed to be located as follows with regard to the cell: secreted. The protein localization is believed to be secreted because both signal-peptide prediction programs predict that this protein has a signal peptide, and neither trans-membrane region prediction program predicts that this protein has a trans-membrane region.

Variant protein HSMUC1A_PEA_(—)1_P56 (SEQ ID NO: 498) also has the following non-silent SNPs (Single Nucleotide Polymorphisms) as listed in Table 23, (given according to their position(s) on the amino acid sequence, with the alternative amino acid(s) listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein HSMUC1A_PEA_(—)1_P56 (SEQ ID NO: 498) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 23 Amino acid mutations SNP position(s) on amino acid Alternative Previously sequence amino acid(s) known SNP? 117 P -> No

Variant protein HSMUC1A_PEA_(—)1_P56 (SEQ ID NO: 498) is encoded by the following transcript(s): HSMUC1A_PEA_(—)1_T42 (SEQ ID NO: 462), for which the sequence(s) is/are given at the end of the application. The coding portion of transcript HSMUC1A_PEA_(—)1_T42 (SEQ ID NO: 462) is shown in bold; this coding portion starts at position 507 and ends at position 890. The transcript also has the following SNPs as listed in Table 24 (given according to their position on the nucleotide sequence, with the alternative nucleic acid listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein HSMUC1A_PEA_(—)1_P56 (SEQ ID NO: 498) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 24 Nucleic acid SNPs SNP position on nucleotide Alternative Previously sequence nucleic acid known SNP? 572 A -> G No 855 C -> No 943 A -> G No 951 C -> T No 957 C -> T Yes 1002 C -> T Yes 1055 C -> T No 1075 C -> T Yes 1181 G -> No 1193 G -> A Yes 1194 C -> No 1194 C -> T No 1283 A -> T No

Variant protein HSMUC1A_PEA_(—)1_P58 (SEQ ID NO: 499) according to the present invention has an amino acid sequence as given at the end of the application; it is encoded by transcript(s) HSMUC1A_PEA_(—)1_T35 (SEQ ID NO: 459). The location of the variant protein was determined according to results from a number of different software programs and analyses, including analyses from SignalP and other specialized programs. The variant protein is believed to be located as follows with regard to the cell: secreted. The protein localization is believed to be secreted because both signal-peptide prediction programs predict that this protein has a signal peptide, and neither trans-membrane region prediction program predicts that this protein has a trans-membrane region.

Variant protein HSMUC1A_PEA_(—)1_P58 (SEQ ID NO: 499) also has the following non-silent SNPs (Single Nucleotide Polymorphisms) as listed in Table 25, (given according to their position(s) on the amino acid sequence, with the alternative amino acid(s) listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein HSMUC1A_PEA_(—)1_P58 (SEQ ID NO: 499) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 25 Amino acid mutations SNP position(s) on amino acid Alternative Previously sequence amino acid(s) known SNP? 147 P -> No

Variant protein HSMUC1A_PEA_(—)1_P58 (SEQ ID NO: 499) is encoded by the following transcript(s): HSMUC1A_PEA_(—)1_T35 (SEQ ID NO: 459), for which the sequence(s) is/are given at the end of the application. The coding portion of transcript HSMUC1A_PEA_(—)1_T35 (SEQ ID NO: 459) is shown in bold; this coding portion starts at position 507 and ends at position 980. The transcript also has the following SNPs as listed in Table 26 (given according to their position on the nucleotide sequence, with the alternative nucleic acid listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein HSMUC1A_PEA_(—)1_P58 (SEQ ID NO: 499) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 26 Nucleic acid SNPs SNP position on nucleotide Alternative Previously sequence nucleic acid known SNP? 572 A -> G No 945 C -> No 1033 A -> G No 1041 C -> T No 1047 C -> T Yes 1092 C -> T Yes 1145 C -> T No 1165 C -> T Yes 1271 G -> No 1283 G -> A Yes 1284 C -> No 1284 C -> T No 1373 A -> T No

Variant protein HSMUC1A_PEA_(—)1_P59 (SEQ ID NO: 500) according to the present invention has an amino acid sequence as given at the end of the application; it is encoded by transcript(s) HSMUC1A_PEA_(—)1_T28 (SEQ ID NO: 453). The location of the variant protein was determined according to results from a number of different software programs and analyses, including analyses from SignalP and other specialized programs. The variant protein is believed to be located as follows with regard to the cell: secreted. The protein localization is believed to be secreted because both signal-peptide prediction programs predict that this protein has a signal peptide, and neither trans-membrane region prediction program predicts that this protein has a trans-membrane region.

Variant protein HSMUC1A_PEA_(—)1_P59 (SEQ ID NO: 500) is encoded by the following transcript(s): HSMUC1A_PEA_(—)1_T28 (SEQ ID NO: 453), for which the sequence(s) is/are given at the end of the application. The coding portion of transcript HSMUC1A_PEA_(—)1_T28 (SEQ ID NO: 453) is shown in bold; this coding portion starts at position 507 and ends at position 794. The transcript also has the following SNPs as listed in Table 27 (given according to their position on the nucleotide sequence, with the alternative nucleic acid listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein HSMUC1A_PEA_(—)1_P59 (SEQ ID NO: 500) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 27 Nucleic acid SNPs SNP position on nucleotide Alternative Previously sequence nucleic acid known SNP? 572 A -> G No 794 G -> A Yes 796 G -> A No 996 C -> No 1084 A -> G No 1092 C -> T No 1098 C -> T Yes 1143 C -> T Yes 1196 C -> T No 1216 C -> T Yes 1322 G -> No 1334 G -> A Yes 1335 C -> No 1335 C -> T No 1424 A -> T No

Variant protein HSMUC1A_PEA_(—)1_P63 (SEQ ID NO: 501) according to the present invention has an amino acid sequence as given at the end of the application; it is encoded by transcript(s) HSMUC1A_PEA_(—)1_T47 (SEQ ID NO: 464). An alignment is given to the known protein (Mucin 1 precursor) at the end of the application. One or more alignments to one or more previously published protein sequences are given at the end of the application. A brief description of the relationship of the variant protein according to the present invention to each such aligned protein is as follows:

Comparison report between HSMUC1A_PEA_(—)1_P63 (SEQ ID NO: 501) and MUC1_HUMAN:

1. An isolated chimeric polypeptide encoding for HSMUC1A_PEA_(—)1_P63 (SEQ ID NO: 501), comprising a first amino acid sequence being at least 90% homologous to MTPGTQSPFFLLLLLTVLTVVTGSGHASSTPGGEKETSATQRSSV corresponding to amino acids 1-45 of MUC1_HUMAN, which also corresponds to amino acids 1-45 of HSMUC1A_PEA_(—)1_P63 (SEQ ID NO: 501), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence EEEVSADQVSVGASGVLGSFKEARNAPSFLSWSFSMGPSK (SEQ ID NO: 1060) corresponding to amino acids 46-85 of HSMUC1A_PEA_(—)1_P63 (SEQ ID NO: 501), wherein said first amino acid sequence and second amino acid sequence are contiguous and in a sequential order.

2. An isolated polypeptide encoding for a tail of HSMUC1A_PEA_(—)1_P63 (SEQ ID NO: 501), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence EEEVSADQVSVGASGVLGSFKEARNAPSFLSWSFSMGPSK (SEQ ID NO: 1060) in HSMUC1A_PEA_(—)1_P63 (SEQ ID NO: 501).

The location of the variant protein was determined according to results from a number of different software programs and analyses, including analyses from SignalP and other specialized programs. The variant protein is believed to be located as follows with regard to the cell: secreted. The protein localization is believed to be secreted because both signal-peptide prediction programs predict that this protein has a signal peptide, and neither trans-membrane region prediction program predicts that this protein has a trans-membrane region.

The glycosylation sites of variant protein HSMUC1A_PEA_(—)1_P63 (SEQ ID NO: 501), as compared to the known protein Mucin 1 precursor, are described in Table 28 (given according to their position(s) on the amino acid sequence in the first column; the second column indicates whether the glycosylation site is present in the variant protein; and the last column indicates whether the position is different on the variant protein).

TABLE 28 Glycosylation site(s) Position(s) on known amino Present in variant Position in acid sequence protein? variant protein? 1055 no 957 no 975 no 1133 no 1029 no

Variant protein HSMUC1A_PEA_(—)1_P63 (SEQ ID NO: 501) is encoded by the following transcript(s): HSMUC1A_PEA_(—)1_T47 (SEQ ID NO: 464), for which the sequence(s) is/are given at the end of the application. The coding portion of transcript HSMUC1A_PEA_(—)1_T47 (SEQ ID NO: 464) is shown in bold; this coding portion starts at position 507 and ends at position 761. The transcript also has the following SNPs as listed in Table 29 (given according to their position on the nucleotide sequence, with the alternative nucleic acid listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein HSMUC1A_PEA_(—)1_P63 (SEQ ID NO: 501) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 29 Nucleic acid SNPs SNP position on nucleotide Alternative Previously sequence nucleic acid known SNP? 572 A -> G No 900 A -> No 904 C -> No 963 A -> C Yes 1211 A -> G No 1219 C -> T No 1225 C -> T Yes 1270 C -> T Yes 1323 C -> T No 1343 C -> T Yes 1449 G -> No 1461 G -> A Yes 1462 C -> No 1462 C -> T No 1551 A -> T No

As noted above, cluster HSMUC1A features 22 segment(s), which were listed in Table 2 above and for which the sequence(s) are given at the end of the application. These segment(s) are portions of nucleic acid sequence(s) which are described herein separately because they are of particular interest. A description of each segment according to the present invention is now provided.

Segment cluster HSMUC1A_PEA_(—)1_node_(—)0 (SEQ ID NO: 465) according to the present invention is supported by 31 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HSMUC1A_PEA_(—)1_T12 (SEQ ID NO: 451), HSMUC1A_PEA_(—)1_T26 (SEQ ID NO: 452), HSMUC1A_PEA_(—)1_T28 (SEQ ID NO: 453), HSMUC1A_PEA_(—)1_T29 (SEQ ID NO: 454), HSMUC1A_PEA_(—)1_T30 (SEQ ID NO: 455), HSMUC1A_PEA_(—)1_T31 (SEQ ID NO: 456), HSMUC1A_PEA_(—)1_T33 (SEQ ID NO: 457), HSMUC1A_PEA_(—)1_T34 (SEQ ID NO: 458), HSMUC1A_PEA_(—)1_T35 (SEQ ID NO: 459), HSMUC1A_PEA_(—)1_T36 (SEQ ID NO: 460), HSMUC1A_PEA_(—)1_T40 (SEQ ID NO: 461), HSMUC1A_PEA_(—)1_T42 (SEQ ID NO: 462), HSMUC1A_PEA_(—)1_T43 (SEQ ID NO: 463) and HSMUC1A_PEA_(—)1_T47 (SEQ ID NO: 464). Table 30 below describes the starting and ending position of this segment on each transcript.

TABLE 30 Segment location on transcripts Segment Segment Transcript starting ending name position position HSMUC1A_PEA_1_T12 1 564 (SEQ ID NO:451) HSMUC1A_PEA_1_T26 1 564 (SEQ ID NO:452) HSMUC1A_PEA_1_T28 1 564 (SEQ ID NO:453) HSMUC1A_PEA_1_T29 1 564 (SEQ ID NO:454) HSMUC1A_PEA_1_T30 1 564 (SEQ ID NO:455) HSMUC1A_PEA_1_T31 1 564 (SEQ ID NO:456) HSMUC1A_PEA_1_T33 1 564 (SEQ ID NO:457) HSMUC1A_PEA_1_T34 1 564 (SEQ ID NO:458) HSMUC1A_PEA_1_T35 1 564 (SEQ ID NO:459) HSMUC1A_PEA_1_T36 1 564 (SEQ ID NO:460) HSMUC1A_PEA_1_T40 1 564 (SEQ ID NO:461) HSMUC1A_PEA_1_T42 1 564 (SEQ ID NO:462) HSMUC1A_PEA_1_T43 1 564 (SEQ ID NO:463) HSMUC1A_PEA_1_T47 1 564 (SEQ ID NO:464)

Segment cluster HSMUC1A_PEA_(—)1_node_(—)14 (SEQ ID NO: 466) according to the present invention is supported by 55 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HSMUC1A_PEA_(—)1_T12 (SEQ ID NO: 451). Table 31 below describes the starting and ending position of this segment on each transcript.

TABLE 31 Segment location on transcripts Segment Segment Transcript starting ending name position position HSMUC1A_PEA_1_T12 666 841 (SEQ ID NO:451)

Segment cluster HSMUC1A_PEA_(—)1_node_(—)24 (SEQ ID NO: 467) according to the present invention is supported by 135 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HSMUC1A_PEA_(—)1_T12 (SEQ ID NO: 451). Table 32 below describes the starting and ending position of this segment on each transcript.

Table 32—Segment Location on Transcripts

TABLE 32 Segment location on transcripts Segment Segment Transcript starting ending name position position HSMUC1A_PEA_1_T12 953 1084 (SEQ ID NO:451)

Segment cluster HSMUC1A_PEA_(—)1_node_(—)29 (SEQ ID NO: 468) according to the present invention is supported by 156 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HSMUC1A_PEA_(—)1_T12 (SEQ ID NO: 451), HSMUC1A_PEA_(—)1_T26 (SEQ ID NO: 452), HSMUC1A_PEA_(—)1_T28 (SEQ ID NO: 453), HSMUC1A_PEA_(—)1_T29 (SEQ ID NO: 454), HSMUC1A_PEA_(—)1_T30 (SEQ ID NO: 455), HSMUC1A_PEA_(—)1_T31 (SEQ ID NO: 456), HSMUC1A_PEA_(—)1_T33 (SEQ ID NO: 457), HSMUC1A_PEA_(—)1_T34 (SEQ ID NO: 458), HSMUC1A_PEA_(—)1_T35 (SEQ ID NO: 459), HSMUC1A_PEA_(—)1_T36 (SEQ ID NO: 460), HSMUC1A_PEA_(—)1_T40 (SEQ ID NO: 461), HSMUC1A_PEA_(—)1_T42 (SEQ ID NO: 462) and HSMUC1A_PEA_(—)1_T43 (SEQ ID NO: 463). Table 33 below describes the starting and ending position of this segment on each transcript.

TABLE 33 Segment location on transcripts Segment Segment Transcript starting ending name position position HSMUC1A_PEA_1_T12 1207 1346 (SEQ ID NO:451) HSMUC1A_PEA_1_T26 894 1033 (SEQ ID NO:452) HSMUC1A_PEA_1_T28 913 1052 (SEQ ID NO:453) HSMUC1A_PEA_1_T29 865 1004 (SEQ ID NO:454) HSMUC1A_PEA_1_T30 838 977 (SEQ ID NO:455) HSMUC1A_PEA_1_T31 788 927 (SEQ ID NO:456) HSMUC1A_PEA_1_T33 881 1020 (SEQ ID NO:457) HSMUC1A_PEA_1_T34 783 922 (SEQ ID NO:458) HSMUC1A_PEA_1_T35 862 1001 (SEQ ID NO:459) HSMUC1A_PEA_1_T36 756 895 (SEQ ID NO:460) HSMUC1A_PEA_1_T40 762 901 (SEQ ID NO:461) HSMUC1A_PEA_1_T42 772 911 (SEQ ID NO:462) HSMUC1A_PEA_1_T43 693 832 (SEQ ID NO:463)

Segment cluster HSMUC1A_PEA_(—)1_node_(—)35 (SEQ ID NO: 469) according to the present invention is supported by 51 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HSMUC1A_PEA_(—)1_T47 (SEQ ID NO: 464). Table 34 below describes the starting and ending position of this segment on each transcript.

TABLE 34 Segment location on transcripts Segment Segment Transcript starting ending name position position HSMUC1A_PEA_1_T47 666 1189 (SEQ ID NO:451)

Microarray (chip) data is also available for this segment as follows. As described above with regard to the cluster itself, various oligonucleotides were tested for being differentially expressed in various disease conditions, particularly cancer. The following oligonucleotides were found to hit this segment (in regard to ovarian cancer), shown in Table 35.

TABLE 35 Oligonucleotides related to this segment Oligonucleotide Overexpressed Chip name in cancers reference HSMUC1A_0_0_11365 ovarian OVA (SEQ ID NO:1030) carcinoma

Segment cluster HSMUC1A_PEA_(—)1_node_(—)38 (SEQ ID NO: 470) according to the present invention is supported by 140 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HSMUC1A_PEA_(—)1_T12 (SEQ ID NO: 451), HSMUC1A_PEA_(—)1_T26 (SEQ ID NO: 452), HSMUC1A_PEA_(—)1_T28 (SEQ ID NO: 453), HSMUC1A_PEA_(—)1_T29 (SEQ ID NO: 454), HSMUC1A_PEA_(—)1_T30 (SEQ ID NO: 455), HSMUC1A_PEA_(—)1_T31 (SEQ ID NO: 456), HSMUC1A_PEA_(—)1_T33 (SEQ ID NO: 457), HSMUC1A_PEA_(—)1_T34 (SEQ ID NO: 458), HSMUC1A_PEA_(—)1_T35 (SEQ ID NO: 459), HSMUC1A_PEA_(—)1_T36 (SEQ ID NO: 460), HSMUC1A_PEA_(—)1_T40 (SEQ ID NO: 461), HSMUC1A_PEA_(—)1_T42 (SEQ ID NO: 462), HSMUC1A_PEA_(—)1_T43 (SEQ ID NO: 463) and HSMUC1A_PEA_(—)1_T47 (SEQ ID NO: 464). Table 36 below describes the starting and ending position of this segment on each transcript.

TABLE 36 Segment location on transcripts Segment Segment Transcript starting ending name position position HSMUC1A_PEA_1_T12 1488 1749 (SEQ ID NO:451) HSMUC1A_PEA_1_T26 1175 1436 (SEQ ID NO:452) HSMUC1A_PEA_1_T28 1194 1455 (SEQ ID NO:453) HSMUC1A_PEA_1_T29 1146 1407 (SEQ ID NO:454) HSMUC1A_PEA_1_T30 1119 1380 (SEQ ID NO:455) HSMUC1A_PEA_1_T31 1069 1330 (SEQ ID NO:456) HSMUC1A_PEA_1_T33 1162 1423 (SEQ ID NO:457) HSMUC1A_PEA_1_T34 1064 1325 (SEQ ID NO:458) HSMUC1A_PEA_1_T35 1143 1404 (SEQ ID NO:459) HSMUC1A_PEA_1_T36 1037 1298 (SEQ ID NO:460) HSMUC1A_PEA_1_T40 1043 1304 (SEQ ID NO:461) HSMUC1A_PEA_1_T42 1053 1314 (SEQ ID NO:462) HSMUC1A_PEA_1_T43 974 1235 (SEQ ID NO:463) HSMUC1A_PEA_1_T47 1321 1582 (SEQ ID NO:464)

According to an optional embodiment of the present invention, short segments related to the above cluster are also provided. These segments are up to about 120 bp in length, and so are included in a separate description.

Segment cluster HSMUC1A_PEA_(—)1_node_(—)3 (SEQ ID NO: 471) according to the present invention is supported by 17 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HSMUC1A_PEA_(—)1_T29 (SEQ ID NO: 454), HSMUC1A_PEA_(—)1_T34 (SEQ ID NO: 458), HSMUC1A_PEA_(—)1_T40 (SEQ ID NO: 461) and HSMUC1A_PEA_(—)1_T43 (SEQ ID NO: 463). Table 37 below describes the starting and ending position of this segment on each transcript.

TABLE 37 Segment location on transcripts Segment Segment Transcript starting ending name position position HSMUC1A_PEA_1_T29 565 591 (SEQ ID NO:454) HSMUC1A_PEA_1_T34 565 591 (SEQ ID NO:458) HSMUC1A_PEA_1_T40 565 591 (SEQ ID NO:461) HSMUC1A_PEA_1_T43 565 591 (SEQ ID NO:463)

Segment cluster HSMUC1A_PEA_(—)1_node_(—)4 (SEQ ID NO: 472) according to the present invention can be found in the following transcript(s): HSMUC1A_PEA_(—)1_T12 (SEQ ID NO: 451), HSMUC1A_PEA_(—)1_T26 (SEQ ID NO: 452), HSMUC1A_PEA_(—)1_T28 (SEQ ID NO: 453), HSMUC1A_PEA_(—)1_T29 (SEQ ID NO: 454), HSMUC1A_PEA_(—)1_T30 (SEQ ID NO: 455), HSMUC1A_PEA_(—)1_T31 (SEQ ID NO: 456), HSMUC1A_PEA_(—)1_T33 (SEQ ID NO: 457), HSMUC1A_PEA_(—)1_T34 (SEQ ID NO: 458), HSMUC1A_PEA_(—)1_T35 (SEQ ID NO: 459), HSMUC1A_PEA_(—)1_T36 (SEQ ID NO: 460), HSMUC1A_PEA_(—)1_T40 (SEQ ID NO: 461), HSMUC1A_PEA_(—)1_T42 (SEQ ID NO: 462), HSMUC1A_PEA_(—)1_T43 (SEQ ID NO: 463) and HSMUC1A_PEA_(—)1_T47 (SEQ ID NO: 464). Table 38 below describes the starting and ending position of this segment on each transcript.

TABLE 38 Segment location on transcripts Segment Segment Transcript starting ending name position position HSMUC1A_PEA_1_T12 565 573 (SEQ ID NO:451) HSMUC1A_PEA_1_T26 565 573 (SEQ ID NO:452) HSMUC1A_PEA_1_T28 565 573 (SEQ ID NO:453) HSMUC1A_PEA_1_T29 592 600 (SEQ ID NO:454) HSMUC1A_PEA_1_T30 565 573 (SEQ ID NO:455) HSMUC1A_PEA_1_T31 565 573 (SEQ ID NO:456) HSMUC1A_PEA_1_T33 565 573 (SEQ ID NO:457) HSMUC1A_PEA_1_T34 592 600 (SEQ ID NO:458) HSMUC1A_PEA_1_T35 565 573 (SEQ ID NO:459) HSMUC1A_PEA_1_T36 565 573 (SEQ ID NO:460) HSMUC1A_PEA_1_T40 592 600 (SEQ ID NO:461) HSMUC1A_PEA_1_T42 565 573 (SEQ ID NO:462) HSMUC1A_PEA_1_T43 592 600 (SEQ ID NO:463) HSMUC1A_PEA_1_T47 565 573 (SEQ ID NO:464)

Segment cluster HSMUC1A_PEA_(—)1_node_(—)5 (SEQ ID NO: 473) according to the present invention is supported by 34 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HSMUC1A_PEA_(—)1_T12 (SEQ ID NO: 451), HSMUC1A_PEA_(—)1_T26 (SEQ ID NO: 452), HSMUC1A_PEA_(—)1_T28 (SEQ ID NO: 453), HSMUC1A_PEA_(—)1_T29 (SEQ ID NO: 454), HSMUC1A_PEA_(—)1_T30 (SEQ ID NO: 455), HSMUC1A_PEA_(—)1_T31 (SEQ ID NO: 456), HSMUC1A_PEA_(—)1_T33 (SEQ ID NO: 457), HSMUC1A_PEA_(—)1_T34 (SEQ ID NO: 458), HSMUC1A_PEA_(—)1_T35 (SEQ ID NO: 459), HSMUC1A_PEA_(—)1_T36 (SEQ ID NO: 460), HSMUC1A_PEA_(—)1_T40 (SEQ ID NO: 461), HSMUC1A_PEA_(—)1_T42 (SEQ ID NO: 462), HSMUC1A_PEA_(—)1_T43 (SEQ ID NO: 463) and HSMUC1A_PEA_(—)1_T47 (SEQ ID NO: 464). Table 39 below describes the starting and ending position of this segment on each transcript.

TABLE 39 Segment location on transcripts Segment Segment Transcript starting ending name position position HSMUC1A_PEA_1_T12 574 600 (SEQ ID NO:451) HSMUC1A_PEA_1_T26 574 600 (SEQ ID NO:452) HSMUC1A_PEA_1_T28 574 600 (SEQ ID NO:453) HSMUC1A_PEA_1_T29 601 627 (SEQ ID NO:454) HSMUC1A_PEA_1_T30 574 600 (SEQ ID NO:455) HSMUC1A_PEA_1_T31 574 600 (SEQ ID NO:456) HSMUC1A_PEA_1_T33 574 600 (SEQ ID NO:457) HSMUC1A_PEA_1_T34 601 627 (SEQ ID NO:458) HSMUC1A_PEA_1_T35 574 600 (SEQ ID NO:459) HSMUC1A_PEA_1_T36 574 600 (SEQ ID NO:460) HSMUC1A_PEA_1_T40 601 627 (SEQ ID NO:461) HSMUC1A_PEA_1_T42 574 600 (SEQ ID NO:462) HSMUC1A_PEA_1_T43 601 627 (SEQ ID NO:463) HSMUC1A_PEA_1_T47 574 600 (SEQ ID NO:464)

Segment cluster HSMUC1A_PEA_(—)1_node_(—)6 (SEQ ID NO: 474) according to the present invention is supported by 35 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HSMUC1A_PEA_(—)1_T12 (SEQ ID NO: 451), HSMUC1A_PEA_(—)1_T26 (SEQ ID NO: 452), HSMUC1A_PEA_(—)1_T28 (SEQ ID NO: 453), HSMUC1A_PEA_(—)1_T29 (SEQ ID NO: 454), HSMUC1A_PEA_(—)1_T30 (SEQ ID NO: 455), HSMUC1A_PEA_(—)1_T31 (SEQ ID NO: 456), HSMUC1A_PEA_(—)1_T33 (SEQ ID NO: 457), HSMUC1A_PEA_(—)1_T34 (SEQ ID NO: 458), HSMUC1A_PEA_(—)1_T35 (SEQ ID NO: 459), HSMUC1A_PEA_(—)1_T36 (SEQ ID NO: 460), HSMUC1A_PEA_(—)1_T40 (SEQ ID NO: 461), HSMUC1A_PEA_(—)1_T42 (SEQ ID NO: 462), HSMUC1A_PEA_(—)1_T43 (SEQ ID NO: 463) and HSMUC1A_PEA_(—)1_T47 (SEQ ID NO: 464). Table 40 below describes the starting and ending position of this segment on each transcript.

TABLE 40 Segment location on transcripts Segment Segment starting ending Transcript name position position HSMUC1A_PEA_1_T12 601 638 (SEQ ID NO: 451) HSMUC1A_PEA_1_T26 601 638 (SEQ ID NO: 452) HSMUC1A_PEA_1_T28 601 638 (SEQ ID NO: 453) HSMUC1A_PEA_1_T29 628 665 (SEQ ID NO: 454) HSMUC1A_PEA_1_T30 601 638 (SEQ ID NO: 455) HSMUC1A_PEA_1_T31 601 638 (SEQ ID NO: 456) HSMUC1A_PEA_1_T33 601 638 (SEQ ID NO: 457) HSMUC1A_PEA_1_T34 628 665 (SEQ ID NO: 458) HSMUC1A_PEA_1_T35 601 638 (SEQ ID NO: 459) HSMUC1A_PEA_1_T36 601 638 (SEQ ID NO: 460) HSMUC1A_PEA_1_T40 628 665 (SEQ ID NO: 461) HSMUC1A_PEA_1_T42 601 638 (SEQ ID NO: 462) HSMUC1A_PEA_1_T43 628 665 (SEQ ID NO: 463) HSMUC1A_PEA_1_T47 601 638 (SEQ ID NO: 464)

Microarray (chip) data is also available for this segment as follows. As described above with regard to the cluster itself, various oligonucleotides were tested for being differentially expressed in various disease conditions, particularly cancer. The following oligonucleotides were found to hit this segment (in regard to ovarian cancer), shown in Table 41.

TABLE 41 Oligonucleotides related to this segment Oligonucleotide Overexpressed name in cancers Chip reference HSMUC1A_0_37_0 ovarian carcinoma OVA (SEQ ID NO: 1028)

Segment cluster HSMUC1A_PEA_(—)1_node_(—)7 (SEQ ID NO: 475) according to the present invention is supported by 32 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HSMUC1A_PEA_(—)1_T12 (SEQ ID NO: 451), HSMUC1A_PEA_(—)1_T26 (SEQ ID NO: 452), HSMUC1A_PEA_(—)1_T28 (SEQ ID NO: 453), HSMUC1A_PEA_(—)1_T29 (SEQ ID NO: 454), HSMUC1A_PEA_(—)1_T30 (SEQ ID NO: 455), HSMUC1A_PEA_(—)1_T31 (SEQ ID NO: 456), HSMUC1A_PEA_(—)1_T33 (SEQ ID NO: 457), HSMUC1A_PEA_(—)1_T34 (SEQ ID NO: 458), HSMUC1A_PEA_(—)1_T35 (SEQ ID NO: 459), HSMUC1A_PEA_(—)1_T36 (SEQ ID NO: 460), HSMUC1A_PEA_(—)1_T40 (SEQ ID NO: 461), HSMUC1A_PEA_(—)1_T42 (SEQ ID NO: 462) and HSMUC1A_PEA_(—)1_T43 (SEQ ID NO: 463). Table 42 below describes the starting and ending position of this segment on each transcript.

TABLE 42 Segment location on transcripts Segment Segment starting ending Transcript name position position HSMUC1A_PEA_1_T12 639 665 (SEQ ID NO: 451) HSMUC1A_PEA_1_T26 639 665 (SEQ ID NO: 452) HSMUC1A_PEA_1_T28 639 665 (SEQ ID NO: 453) HSMUC1A_PEA_1_T29 666 692 (SEQ ID NO: 454) HSMUC1A_PEA_1_T30 639 665 (SEQ ID NO: 455) HSMUC1A_PEA_1_T31 639 665 (SEQ ID NO: 456) HSMUC1A_PEA_1_T33 639 665 (SEQ ID NO: 457) HSMUC1A_PEA_1_T34 666 692 (SEQ ID NO: 458) HSMUC1A_PEA_1_T35 639 665 (SEQ ID NO: 459) HSMUC1A_PEA_1_T36 639 665 (SEQ ID NO: 460) HSMUC1A_PEA_1_T40 666 692 (SEQ ID NO: 461) HSMUC1A_PEA_1_T42 639 665 (SEQ ID NO: 462) HSMUC1A_PEA_1_T43 666 692 (SEQ ID NO: 463)

Microarray (chip) data is also available for this segment as follows. As described above with regard to the cluster itself, various oligonucleotides were tested for being differentially expressed in various disease conditions, particularly cancer. The following oligonucleotides were found to hit this segment (in regard to ovarian cancer), shown in Table 43.

TABLE 43 Oligonucleotides related to this segment Oligonucleotide Overexpressed name in cancers Chip reference HSMUC1A_0_37_0 ovarian carcinoma OVA (SEQ ID NO: 1028)

Segment cluster HSMUC1A_PEA_(—)1_node_(—)17 (SEQ ID NO: 476) according to the present invention can be found in the following transcript(s): HSMUC1A_PEA_(—)1_T28 (SEQ ID NO: 453), HSMUC1A_PEA_(—)1_T33 (SEQ ID NO: 457) and HSMUC1A_PEA_(—)1_T40 (SEQ ID NO: 461). Table 44 below describes the starting and ending position of this segment on each transcript.

TABLE 44 Segment location on transcripts Segment Segment starting ending Transcript name position position HSMUC1A_PEA_1_T28 666 684 (SEQ ID NO: 453) HSMUC1A_PEA_1_T33 666 684 (SEQ ID NO: 457) HSMUC1A_PEA_1_T40 693 711 (SEQ ID NO: 461)

Segment cluster HSMUC1A_PEA_(—)1_node_(—)18 (SEQ ID NO: 477) according to the present invention is supported by 90 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HSMUC1A_PEA_(—)1_T12 (SEQ ID NO: 451), HSMUC1A_PEA_(—)1_T26 (SEQ ID NO: 452), HSMUC1A_PEA_(—)1_T28 (SEQ ID NO: 453), HSMUC1A_PEA_(—)1_T29 (SEQ ID NO: 454), HSMUC1A_PEA_(—)1_T30 (SEQ ID NO: 455), HSMUC1A_PEA_(—)1_T33 (SEQ ID NO: 457), HSMUC1A_PEA_(—)1_T35 (SEQ ID NO: 459), HSMUC1A_PEA_(—)1_T40 (SEQ ID NO: 461) and HSMUC1A_PEA_(—)1_T42 (SEQ ID NO: 462). Table 45 below describes the starting and ending position of this segment on each transcript.

TABLE 45 Segment location on transcripts Segment Segment starting ending Transcript name position position HSMUC1A_PEA_1_T12 842 891 (SEQ ID NO: 451) HSMUC1A_PEA_1_T26 666 715 (SEQ ID NO: 452) HSMUC1A_PEA_1_T28 685 734 (SEQ ID NO: 453) HSMUC1A_PEA_1_T29 693 742 (SEQ ID NO: 454) HSMUC1A_PEA_1_T30 666 715 (SEQ ID NO: 455) HSMUC1A_PEA_1_T33 685 734 (SEQ ID NO: 457) HSMUC1A_PEA_1_T35 666 715 (SEQ ID NO: 459) HSMUC1A_PEA_1_T40 712 761 (SEQ ID NO: 461) HSMUC1A_PEA_1_T42 666 715 (SEQ ID NO: 462)

Segment cluster HSMUC1A_PEA_(—)1_node_(—)20 (SEQ ID NO: 478) according to the present invention can be found in the following transcript(s): HSMUC1A_PEA_(—)1_T12 (SEQ ID NO: 451), HSMUC1A_PEA_(—)1_T26 (SEQ ID NO: 452), HSMUC1A_PEA_(—)1_T28 (SEQ ID NO: 453), HSMUC1A_PEA_(—)1_T33 (SEQ ID NO: 457), HSMUC1A_PEA_(—)1_T35 (SEQ ID NO: 459) and HSMUC1A_PEA_(—)1_T42 (SEQ ID NO: 462). Table 46 below describes the starting and ending position of this segment on each transcript.

TABLE 46 Segment location on transcripts Segment Segment starting ending Transcript name position position HSMUC1A_PEA_1_T12 892 900 (SEQ ID NO: 451) HSMUC1A_PEA_1_T26 716 724 (SEQ ID NO: 452) HSMUC1A_PEA_1_T28 735 743 (SEQ ID NO: 453) HSMUC1A_PEA_1_T33 735 743 (SEQ ID NO: 457) HSMUC1A_PEA_1_T35 716 724 (SEQ ID NO: 459) HSMUC1A_PEA_1_T42 716 724 (SEQ ID NO: 462)

Segment cluster HSMUC1A_PEA_(—)1_node_(—)21 (SEQ ID NO: 479) according to the present invention is supported by 97 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HSMUC1A_PEA_(—)1_T12 (SEQ ID NO: 451), HSMUC1A_PEA_(—)1_T26 (SEQ ID NO: 452), HSMUC1A_PEA_(—)1_T28 (SEQ ID NO: 453), HSMUC1A_PEA_(—)1_T33 (SEQ ID NO: 457), HSMUC1A_PEA_(—)1_T35 (SEQ ID NO: 459) and HSMUC1A_PEA_(—)1_T42 (SEQ ID NO: 462). Table 47 below describes the starting and ending position of this segment on each transcript.

TABLE 47 Segment location on transcripts Segment Segment starting ending Transcript name position position HSMUC1A_PEA_1_T12 901 947 (SEQ ID NO: 451) HSMUC1A_PEA_1_T26 725 771 (SEQ ID NO: 452) HSMUC1A_PEA_1_T28 744 790 (SEQ ID NO: 453) HSMUC1A_PEA_1_T33 744 790 (SEQ ID NO: 457) HSMUC1A_PEA_1_T35 725 771 (SEQ ID NO: 459) HSMUC1A_PEA_1_T42 725 771 (SEQ ID NO: 462)

Segment cluster HSMUC1A_PEA_(—)1_node_(—)23 (SEQ ID NO: 480) according to the present invention can be found in the following transcript(s): HSMUC1A_PEA_(—)1_T12 (SEQ ID NO: 451). Table 48 below describes the starting and ending position of this segment on each transcript.

TABLE 48 Segment location on transcripts Segment Segment starting ending Transcript name position position HSMUC1A_PEA_1_T12 948 952 (SEQ ID NO: 451)

Segment cluster HSMUC1A_PEA_(—)1_node_(—)26 (SEQ ID NO: 481) according to the present invention is supported by 129 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HSMUC1A_PEA_(—)1_T12 (SEQ ID NO: 451), HSMUC1A_PEA_(—)1_T26 (SEQ ID NO: 452), HSMUC1A_PEA_(—)1_T28 (SEQ ID NO: 453), HSMUC1A_PEA_(—)1_T29 (SEQ ID NO: 454), HSMUC1A_PEA_(—)1_T30 (SEQ ID NO: 455) and HSMUC1A_PEA_(—)1_T31 (SEQ ID NO: 456). Table 49 below describes the starting and ending position of this segment on each transcript.

TABLE 49 Segment location on transcripts Segment Segment starting ending Transcript name position position HSMUC1A_PEA_1_T12 1085 1116 (SEQ ID NO: 451) HSMUC1A_PEA_1_T26 772 803 (SEQ ID NO: 452) HSMUC1A_PEA_1_T28 791 822 (SEQ ID NO: 453) HSMUC1A_PEA_1_T29 743 774 (SEQ ID NO: 454) HSMUC1A_PEA_1_T30 716 747 (SEQ ID NO: 455) HSMUC1A_PEA_1_T31 666 697 (SEQ ID NO: 456)

Segment cluster HSMUC1A_PEA_(—)1_node_(—)27 (SEQ ID NO: 482) according to the present invention is supported by 140 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HSMUC1A_PEA_(—)1_T12 (SEQ ID NO: 451), HSMUC1A_PEA_(—)1_T26 (SEQ ID NO: 452), HSMUC1A_PEA_(—)1_T28 (SEQ ID NO: 453), HSMUC1A_PEA_(—)1_T29 (SEQ ID NO: 454), HSMUC1A_PEA_(—)1_T30 (SEQ ID NO: 455), HSMUC1A_PEA_(—)1_T31 (SEQ ID NO: 456), HSMUC1A_PEA_(—)1_T33 (SEQ ID NO: 457), HSMUC1A_PEA_(—)1_T34 (SEQ ID NO: 458), HSMUC1A_PEA_(—)1_T35 (SEQ ID NO: 459) and HSMUC1A_PEA_(—)1_T36 (SEQ ID NO: 460). Table 50 below describes the starting and ending position of this segment on each transcript.

TABLE 50 Segment location on transcripts Segment Segment starting ending Transcript name position position HSMUC1A_PEA_1_T12 1117 1206 (SEQ ID NO: 451) HSMUC1A_PEA_1_T26 804 893 (SEQ ID NO: 452) HSMUC1A_PEA_1_T28 823 912 (SEQ ID NO: 453) HSMUC1A_PEA_1_T29 775 864 (SEQ ID NO: 454) HSMUC1A_PEA_1_T30 748 837 (SEQ ID NO: 455) HSMUC1A_PEA_1_T31 698 787 (SEQ ID NO: 456) HSMUC1A_PEA_1_T33 791 880 (SEQ ID NO: 457) HSMUC1A_PEA_1_T34 693 782 (SEQ ID NO: 458) HSMUC1A_PEA_1_T35 772 861 (SEQ ID NO: 459) HSMUC1A_PEA_1_T36 666 755 (SEQ ID NO: 460)

Segment cluster HSMUC1A_PEA_(—)1_node_(—)31 (SEQ ID NO: 483) according to the present invention can be found in the following transcript(s): HSMUC1A_PEA_(—)1_T12 (SEQ ID NO: 451), HSMUC1A_PEA_(—)1_T26 (SEQ ID NO: 452), HSMUC1A_PEA_(—)1_T28 (SEQ ID NO: 453), HSMUC1A_PEA_(—)1_T29 (SEQ ID NO: 454), HSMUC1A_PEA_(—)1_T30 (SEQ ID NO: 455), HSMUC1A_PEA_(—)1_T31 (SEQ ID NO: 456), HSMUC1A_PEA_(—)1_T33 (SEQ ID NO: 457), HSMUC1A_PEA_(—)1_T34 (SEQ ID NO: 458), HSMUC1A_PEA_(—)1_T35 (SEQ ID NO: 459), HSMUC1A_PEA_(—)1_T36 (SEQ ID NO: 460), HSMUC1A_PEA_(—)1_T40 (SEQ ID NO: 461), HSMUC1A_PEA_(—)1_T42 (SEQ ID NO: 462) and HSMUC1A_PEA_(—)1_T43 (SEQ ID NO: 463). Table 51 below describes the starting and ending position of this segment on each transcript.

TABLE 51 Segment location on transcripts Segment Segment starting ending Transcript name position position HSMUC1A_PEA_1_T12 1347 1356 (SEQ ID NO: 451) HSMUC1A_PEA_1_T26 1034 1043 (SEQ ID NO: 452) HSMUC1A_PEA_1_T28 1053 1062 (SEQ ID NO: 453) HSMUC1A_PEA_1_T29 1005 1014 (SEQ ID NO: 454) HSMUC1A_PEA_1_T30 978 987 (SEQ ID NO: 455) HSMUC1A_PEA_1_T31 928 937 (SEQ ID NO: 456) HSMUC1A_PEA_1_T33 1021 1030 (SEQ ID NO: 457) HSMUC1A_PEA_1_T34 923 932 (SEQ ID NO: 458) HSMUC1A_PEA_1_T35 1002 1011 (SEQ ID NO: 459) HSMUC1A_PEA_1_T36 896 905 (SEQ ID NO: 460) HSMUC1A_PEA_1_T40 902 911 (SEQ ID NO: 461) HSMUC1A_PEA_1_T42 912 921 (SEQ ID NO: 462) HSMUC1A_PEA_1_T43 833 842 (SEQ ID NO: 463)

Segment cluster HSMUC1A_PEA_(—)1_node_(—)34 (SEQ ID NO: 484) according to the present invention is supported by 24 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HSMUC1A_PEA_(—)1_T47 (SEQ ID NO: 464). Table 52 below describes the starting and ending position of this segment on each transcript.

TABLE 52 Segment location on transcripts Segment Segment starting ending Transcript name position position HSMUC1A_PEA_1_T47 639 665 (SEQ ID NO: 464)

Segment cluster HSMUC1A_PEA_(—)1_node_(—)36 (SEQ ID NO: 485) according to the present invention is supported by 135 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HSMUC1A_PEA_(—)1_T12 (SEQ ID NO: 451), HSMUC1A_PEA_(—)1_T26 (SEQ ID NO: 452), HSMUC1A_PEA_(—)1_T28 (SEQ ID NO: 453), HSMUC1A_PEA_(—)1_T29 (SEQ ID NO: 454), HSMUC1A_PEA_(—)1_T30 (SEQ ID NO: 455), HSMUC1A_PEA_(—)1_T31 (SEQ ID NO: 456), HSMUC1A_PEA_(—)1_T33 (SEQ ID NO: 457), HSMUC1A_PEA_(—)1_T34 (SEQ ID NO: 458), HSMUC1A_PEA_(—)1_T35 (SEQ ID NO: 459), HSMUC1A_PEA_(—)1_T36 (SEQ ID NO: 460), HSMUC1A_PEA_(—)1_T40 (SEQ ID NO: 461), HSMUC1A_PEA_(—)1_T42 (SEQ ID NO: 462), HSMUC1A_PEA_(—)1_T43 (SEQ ID NO: 463) and HSMUC1A_PEA_(—)1_T47 (SEQ ID NO: 464). Table 53 below describes the starting and ending position of this segment on each transcript.

TABLE 53 Segment location on transcripts Segment Segment starting ending Transcript name position position HSMUC1A_PEA_1_T12 1357 1388 (SEQ ID NO: 451) HSMUC1A_PEA_1_T26 1044 1075 (SEQ ID NO: 452) HSMUC1A_PEA_1_T28 1063 1094 (SEQ ID NO: 453) HSMUC1A_PEA_1_T29 1015 1046 (SEQ ID NO: 454) HSMUC1A_PEA_1_T30 988 1019 (SEQ ID NO: 455) HSMUC1A_PEA_1_T31 938 969 (SEQ ID NO: 456) HSMUC1A_PEA_1_T33 1031 1062 (SEQ ID NO: 457) HSMUC1A_PEA_1_T34 933 964 (SEQ ID NO: 458) HSMUC1A_PEA_1_T35 1012 1043 (SEQ ID NO: 459) HSMUC1A_PEA_1_T36 906 937 (SEQ ID NO: 460) HSMUC1A_PEA_1_T40 912 943 (SEQ ID NO: 461) HSMUC1A_PEA_1_T42 922 953 (SEQ ID NO: 462) HSMUC1A_PEA_1_T43 843 874 (SEQ ID NO: 463) HSMUC1A_PEA_1_T47 1190 1221 (SEQ ID NO: 464)

Segment cluster HSMUC1A_PEA_(—)1_node_(—)37 (SEQ ID NO: 486) according to the present invention is supported by 146 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HSMUC1A_PEA_(—)1_T12 (SEQ ID NO: 451), HSMUC1A_PEA_(—)1_T26 (SEQ ID NO: 452), HSMUC1A_PEA_(—)1_T28 (SEQ ID NO: 453), HSMUC1A_PEA_(—)1_T29 (SEQ ID NO: 454), HSMUC1A_PEA_(—)1_T30 (SEQ ID NO: 455), HSMUC1A_PEA_(—)1_T31 (SEQ ID NO: 456), HSMUC1A_PEA_(—)1_T33 (SEQ ID NO: 457), HSMUC1A_PEA_(—)1_T34 (SEQ ID NO: 458), HSMUC1A_PEA_(—)1_T35 (SEQ ID NO: 459), HSMUC1A_PEA_(—)1_T36 (SEQ ID NO: 460), HSMUC1A_PEA_(—)1_T40 (SEQ ID NO: 461), HSMUC1A_PEA_(—)1_T42 (SEQ ID NO: 462), HSMUC1A_PEA_(—)1_T43 (SEQ ID NO: 463) and HSMUC1A_PEA_(—)1_T47 (SEQ ID NO: 464). Table 54 below describes the starting and ending position of this segment on each transcript.

TABLE 54 Segment location on transcripts Segment Segment starting ending Transcript name position position HSMUC1A_PEA_1_T12 1389 1487 (SEQ ID NO: 451) HSMUC1A_PEA_1_T26 1076 1174 (SEQ ID NO: 452) HSMUC1A_PEA_1_T28 1095 1193 (SEQ ID NO: 453) HSMUCIA_PEA_1_T29 1047 1145 (SEQ ID NO: 454) HSMUC1A_PEA_1_T30 1020 1118 (SEQ ID NO: 455) HSMUC1A_PEA_1_T31 970 1068 (SEQ ID NO: 456) HSMUC1A_PEA_1_T33 1063 1161 (SEQ ID NO: 457) HSMUC1A_PEA_1_T34 965 1063 (SEQ ID NO: 458) HSMUC1A_PEA_1_T35 1044 1142 (SEQ ID NO: 459) HSMUC1A_PEA_1_T36 938 1036 (SEQ ID NO: 460) HSMUC1A_PEA_1_T40 944 1042 (SEQ ID NO: 461) HSMUC1A_PEA_1_T42 954 1052 (SEQ ID NO: 462) HSMUC1A_PEA_1_T43 875 973 (SEQ ID NO: 463) HSMUC1A_PEA_1_T47 1222 1320 (SEQ ID NO: 464) Variant protein alignment to the previously known protein: Sequence name: MUC1_HUMAN Sequence documentation:

Alignment of: HSMUC1A_PEA_(—)1_P63 (SEQ ID NO: 501) x MUC1_HUMAN ..

Alignment segment 1/1:

Quality: 429.00 Escore: 0 Matching length: 59 Total length: 59 Matching Percent Similarity: 86.44 Matching Percent Identity: 81.36 Total Percent Similarity: 86.44 Total Percent Identity: 81.36 Gaps: 0

Alignment:

Combined expression of 6 sequences (T10888-junc11-17 (SEQ ID NO: 962); R11723-seg13 (SEQ ID NO: 975); H61775-seg8-F2R2 (SEQ ID NO: 957); Z44808-junc8-11 (SEQ ID NO: 1006); Z25299-seg20 (SEQ ID NO: 996); Z25299-seg23 (SEQ ID NO: 999)) in normal and cancerous ovary tissues

Expression of CEA6_HUMAN Carcinoembryonic antigen-related cell adhesion molecule 6; R11723-hypothetical protein PSEC0181 (PSEC); immunoglobulin superfamily, member 9; SMO2_HUMAN SPARC related modular calcium-binding protein 2 precursor; Secretory leukocyte protease inhibitor Acid-stable proteinase inhibitor; transcripts detectable by or according to the amplicons: T10888-junc11-17 (SEQ ID NO: 962); R11723-seg13 (SEQ ID NO: 975); H61775-seg8-F2R2 (SEQ ID NO: 957); Z44808-junc8-11 (SEQ ID NO: 1006); Z25299-seg20 (SEQ ID NO: 996); Z25299-seg23 (SEQ ID NO: 999) amplicon(s) and the primers: T10888-junc11-17-F (SEQ ID NO: 960) and T10888-junc11-17-R (SEQ ID NO: 961); R11723-seg1-F (SEQ ID NO: 973) and R11723-seg13-R (SEQ ID NO: 974); H61775-seg8-F2 (SEQ ID NO: 955) and H61775-seg8-R2 (SEQ ID NO: 956); Z44808-junc8-11-F (SEQ ID NO: 1004) and Z44808-junc8-11-R (SEQ ID NO: 1005); Z25299-seg20-F (SEQ ID NO: 994) and Z25299-seg20-R (SEQ ID NO: 995); Z25299-seg23-F (SEQ ID NO: 997) and Z25299-seg23-R (SEQ ID NO: 998), was measured by real time PCR. In parallel the expression of four housekeeping genes —PBGD (GenBank Accession No. BC019323 (SEQ ID NO:1036); amplicon—PBGD-amplicon (SEQ ID NO:1039)), HPRT1 (GenBank Accession No. NM_(—)000194 (SEQ ID NO:1040); amplicon—HPRT1-amplicon (SEQ ID NO:1044) and SDHA (GenBank Accession No. NM_(—)004168 (SEQ ID NO: 1032); amplicon—SDHA-amplicon (SEQ ID NO:1035)), GAPDH (GenBank Accession No. BC026907; GAPDH amplicon (SEQ ID NO:1047)) was measured similarly. For each RT sample, the expression of the above amplicons was normalized to the geometric mean of the quantities of the housekeeping genes. The normalized quantity of each RT sample of each amplicon was then divided by the median of the quantities of the normal post-mortem (PM) samples detected for the same amplicon (Sample Nos. 45-48, 71 Table 1, “Tissue samples in testing sample”, above), to obtain a value of fold up-regulation for each sample relative to median of the normal PM samples. The reciprocal of this ratio was calculated for Z44808-junc8-11 (SEQ ID NO: 1006), to obtain a value of fold down-regulation for each sample relative to median of the normal PM samples.

FIG. 43 is a histogram showing differential expression of the above-indicated transcripts in cancerous ovary samples relative to the normal samples. The number and percentage of samples that exhibit at least 10 fold differential of at least one of the sequences, out of the total number of samples tested is indicated in the bottom.

As is evident from FIG. 43, differential expression of at least 10 fold in at least one of the sequences was found in 42 out of 43 cancerous samples.

Description for Cluster HUMCEA

Cluster HUMCEA features 5 transcript(s) and 42 segment(s) of interest, the names for which are given in Tables 1 and 2, respectively, the sequences themselves are given at the end of the application. The selected protein variants are given in table 3.

TABLE 1 Transcripts of interest SEQ ID Transcript Name NO: HUMCEA_PEA_1_T8 502 HUMCEA_PEA_1_T9 503 HUMCEA_PEA_1_T20 504 HUMCEA_PEA_1_T25 505 HUMCEA_PEA_1_T26 506

TABLE 2 Segments of interest SEQ ID Segment Name NO: HUMCEA_PEA_1_node_0 507 HUMCEA_PEA_1_node_2 508 HUMCEA_PEA_1_node_11 509 HUMCEA_PEA_1_node_12 510 HUMCEA_PEA_1_node_31 511 HUMCEA_PEA_1_node_36 512 HUMCEA_PEA_1_node_44 513 HUMCEA_PEA_1_node_46 514 HUMCEA_PEA_1_node_63 515 HUMCEA_PEA_1_node_65 516 HUMCEA_PEA_1_node_67 517 HUMCEA_PEA_1_node_3 518 HUMCEA_PEA_1_node_7 519 HUMCEA_PEA_1_node_8 520 HUMCEA_PEA_1_node_9 521 HUMCEA_PEA_1_node_10 522 HUMCEA_PEA_1_node_15 523 HUMCEA_PEA_1_node_16 524 HUMCEA_PEA_1_node_17 525 HUMCEA_PEA_1_node_18 526 HUMCEA_PEA_1_node_19 527 HUMCEA_PEA_1_node_20 528 HUMCEA_PEA_1_node_21 529 HUMCEA_PEA_1_node_22 530 HUMCEA_PEA_1_node_23 531 HUMCEA_PEA_1_node_24 532 HUMCEA_PEA_1_node_27 533 HUMCEA_PEA_1_node_29 534 HUMCEA_PEA_1_node_30 535 HUMCEA_PEA_1_node_33 536 HUMCEA_PEA_1_node_34 537 HUMCEA_PEA_1_node_35 538 HUMCEA_PEA_1_node_45 539 HUMCEA_PEA_1_node_50 540 HUMCEA_PEA_1_node_51 541 HUMCEA_PEA_1_node_56 542 HUMCEA_PEA_1_node_57 543 HUMCEA_PEA_1_node_58 544 HUMCEA_PEA_1_node_60 545 HUMCEA_PEA_1_node_61 546 HUMCEA_PEA_1_node_62 547 HUMCEA_PEA_1_node_64 548

TABLE 3 Proteins of interest SEQ ID Corresponding Protein Name NO: Transcript(s) HUMCEA_PEA_1_P4 550 HUMCEA_PEA_1_T8 (SEQ ID NO:502) HUMCEA_PEA_1_P5 551 HUMCEA_PEA_1_T9 (SEQ ID NO:503) HUMCEA_PEA_1_P14 552 HUMCEA_PEA_1_T20 (SEQ ID NO:504) HUMCEA_PEA_1_P19 553 HUMCEA_PEA_1_T25 (SEQ ID NO:505) HUMCEA_PEA_1_P20 554 HUMCEA_PEA_1_T26 (SEQ ID NO:506)

These sequences are variants of the known protein Carcinoembryonic antigen-related cell adhesion molecule 5 precursor (SwissProt accession identifier CEA5_HUMAN; known also according to the synonyms Carcinoembryonic antigen; CEA; Meconium antigen 100; CD66e antigen), SEQ ID NO: 549, referred to herein as the previously known protein.

The sequence for protein Carcinoembryonic antigen-related cell adhesion molecule 5 precursor is given at the end of the application, as “Carcinoembryonic antigen-related cell adhesion molecule 5 precursor amino acid sequence”. Known polymorphisms for this sequence are as shown in Table 4.

TABLE 4 Amino acid mutations for Known Protein SNP position(s) on amino acid sequence Comment 320 Missing

Protein Carcinoembryonic antigen-related cell adhesion molecule 5 precursor localization is believed to be Attached to the membrane by a GPI-anchor.

The previously known protein also has the following indication(s) and/or potential therapeutic use(s): Cancer. It has been investigated for clinical/therapeutic use in humans, for example as a target for an antibody or small molecule, and/or as a direct therapeutic; available information related to these investigations is as follows. Potential pharmaceutically related or therapeutically related activity or activities of the previously known protein are as follows: Immunostimulant. A therapeutic role for a protein represented by the cluster has been predicted. The cluster was assigned this field because there was information in the drug database or the public databases (e.g., described herein above) that this protein, or part thereof, is used or can be used for a potential therapeutic indication: Imaging agent; Anticancer; Immunostimulant; Immunoconjugate; Monoclonal antibody, murine; Antisense therapy; antibody.

The following GO Annotation(s) apply to the previously known protein. The following annotation(s) were found: integral plasma membrane protein; membrane, which are annotation(s) related to Cellular Component.

The GO assignment relies on information from one or more of the SwissProt/TremBl Protein knowledgebase, available from <http://www.expasy.ch/sprot/>; or Locuslink, available from <http://www.ncbi.nlm.nih.gov/projects/LocusLink/>.

Cluster HUMCEA can be used as a diagnostic marker according to overexpression of transcripts of this cluster in cancer. Expression of such transcripts in normal tissues is also given according to the previously described methods. The term “number” in the left hand column of the table and the numbers on the y-axis of FIG. 44 below refer to weighted expression of ESTs in each category, as “parts per million” (ratio of the expression of ESTs for a particular cluster to the expression of all ESTs in that category, according to parts per million).

Overall, the following results were obtained as shown with regard to the histograms in FIG. 44 and Table 5. This cluster is overexpressed (at least at a minimum level) in the following pathological conditions: epithelial malignant tumors, a mixture of malignant tumors from different tissues and pancreas carcinoma.

TABLE 5 Normal tissue distribution Name of Tissue Number colon 1175 epithelial 92 general 29 head and neck 81 kidney 0 lung 0 lymph nodes 0 breast 0 pancreas 0 prostate 0 stomach 256

TABLE 6 P values and ratios for expression in cancerous tissue Name of Tissue P1 P2 SP1 R3 SP2 R4 colon 2.0e−01 2.7e−01 9.8e−01 0.5 1 0.5 epithelial 2.1e−03 2.7e−02 6.4e−04 1.4 2.1e−01 1.0 general 3.9e−08 8.2e−06 9.2e−18 3.2 1.3e−10 2.2 head and neck 3.4e−01 5.0e−01 2.1e−01 1.8 5.6e−01 0.9 kidney 4.3e−01 5.3e−01 5.8e−01 2.1 7.0e−01 1.6 lung 1.3e−01 2.6e−01 1 1.1 1 1.1 lymph nodes 3.1e−01 5.7e−01 8.1e−02 6.0 3.3e−01 2.5 breast 3.8e−01 1.5e−01 1 1.0 6.8e−01 1.5 pancreas 2.2e−02 2.3e−02 1.4e−08 7.8 7.4e−07 6.4 prostate 5.3e−01 6.0e−01 3.0e−01 2.5 4.2e−01 2.0 stomach 1.5e−01 4.7e−01 8.9e−01 0.6 7.2e−01 0.4

As noted above, cluster HUMCEA features 5 transcript(s), which were listed in Table 1 above. These transcript(s) encode for protein(s) which are variant(s) of protein Carcinoembryonic antigen-related cell adhesion molecule 5 precursor. A description of each variant protein according to the present invention is now provided.

Variant protein HUMCEA_PEA_(—)1_P4 (SEQ ID NO: 550) according to the present invention has an amino acid sequence as given at the end of the application; it is encoded by transcript(s) HUMCEA_PEA_(—)1_T8 (SEQ ID NO: 502). An alignment is given to the known protein (Carcinoembryonic antigen-related cell adhesion molecule 5 precursor) at the end of the application. One or more alignments to one or more previously published protein sequences are given at the end of the application. A brief description of the relationship of the variant protein according to the present invention to each such aligned protein is as follows:

Comparison report between HUMCEA_PEA_(—)1_P4 (SEQ ID NO: 550) and CEA5_HUMAN:

1. An isolated chimeric polypeptide encoding for HUMCEA_PEA_(—)1_P4 (SEQ ID NO: 550), comprising a first amino acid sequence being at least 90% homologous to MESPSAPPHRWCIPWQRLLLTASLLTFWNPPTTAKLTIESTPFNVAEGKEVLLLVHNLPQ HLFGYSWYKGERVDGNRQIIGYVIGTQQATPGPAYSGREIIYPNASLLIQNIIQNDTGFYT LHVIKSDLVNEEATGQFRVYPELPKPSISSNNSKPVEDKDAVAFTCEPETQDATYLWWV NNQSLPVSPRLQLSNGNRTLTLFNVTRNDTASYKCETQNPVSARRSDSVILNVL corresponding to amino acids 1-234 of CEA5_HUMAN, which also corresponds to amino acids 1-234 of HUMCEA_PEA_(—)1_P4 (SEQ ID NO: 550), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence CEYICSSLAQAASPNPQGQRQDFSVPLRFKYTDPQPWTSRLSVTFCPRKTWADQVLTKN RRGGAASVLGGSGSTPYDGRNR (SEQ ID NO: 1145) corresponding to amino acids 235-315 of HUMCEA_PEA_(—)1_P4 (SEQ ID NO: 550), wherein said first amino acid sequence and second amino acid sequence are contiguous and in a sequential order.

2. An isolated polypeptide encoding for a tail of HUMCEA_PEA_(—)1_P4 (SEQ ID NO: 550), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence CEYICSSLAQAASPNPQGQRQDFSVPLRFKYTDPQPWTSRLSVTFCPRKTWADQVLTKN RRGGAASVLGGSGSTPYDGRNR (SEQ ID NO: 1145) in HUMCEA_PEA_(—)1_P4 (SEQ ID NO: 550).

The location of the variant protein was determined according to results from a number of different software programs and analyses, including analyses from SignalP and other specialized programs. The variant protein is believed to be located as follows with regard to the cell: secreted. The protein localization is believed to be secreted because both signal-peptide prediction programs predict that this protein has a signal peptide, and neither trans-membrane region prediction program predicts that this protein has a trans-membrane region.

Variant protein HUMCEA_PEA_(—)1_P4 (SEQ ID NO: 550) also has the following non-silent SNPs (Single Nucleotide Polymorphisms) as listed in Table 8, (given according to their position(s) on the amino acid sequence, with the alternative amino acid(s) listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein HUMCEA_PEA_(—)1_P4 (SEQ ID NO: 550) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 8 Amino acid mutations SNP position(s) on Alternative Previously amino acid sequence amino acid(s) known SNP? 63 F -> L No 80 I -> V Yes 83 V -> A Yes 137 Q -> P Yes 173 D -> N No

The glycosylation sites of variant protein HUMCEA_PEA_(—)1_P4 (SEQ ID NO: 550), as compared to the known protein Carcinoembryonic antigen-related cell adhesion molecule 5 precursor, are described in Table 9 (given according to their position(s) on the amino acid sequence in the first column; the second column indicates whether the glycosylation site is present in the variant protein; and the last column indicates whether the position is different on the variant protein).

TABLE 9 Glycosylation site(s) Position(s) on known Present in Position in amino acid sequence variant protein? variant protein? 197 yes 197 466 no 360 no 288 no 665 no 560 no 650 no 480 no 104 yes 104 580 no 204 yes 204 115 yes 115 208 yes 208 152 yes 152 309 no 432 no 351 no 246 no 182 yes 182 612 no 256 no 508 no 330 no 274 no 292 no 553 no 529 no 375 no

Variant protein HUMCEA_PEA_(—)1_P4 (SEQ ID NO: 550) is encoded by the following transcript(s): HUMCEA_PEA_(—)1_T8 (SEQ ID NO: 502), for which the sequence(s) is/are given at the end of the application. The coding portion of transcript HUMCEA_PEA_(—)1_T8 (SEQ ID NO: 502) is shown in bold; this coding portion starts at position 115 and ends at position 1059. The transcript also has the following SNPs as listed in Table 10 (given according to their position on the nucleotide sequence, with the alternative nucleic acid listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein HUMCEA_PEA_(—)1_P4 (SEQ ID NO: 550) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 10 Nucleic acid SNPs SNP position on Alternative Previously nucleotide sequence nucleic acid known SNP? 49 T -> No 273 A -> C Yes 303 T -> G No 324 T -> C Yes 352 A -> G Yes 362 T -> C Yes 524 A -> C Yes 631 G -> A No 1315 A -> G No 1380 T -> C No 1533 C -> A Yes 1706 G -> A Yes 2308 T -> C No 2362 C -> T No 2455 A -> No 2504 C -> A Yes 2558 G -> No 2623 G -> No 2639 T -> A No 2640 T -> A No 2832 G -> A Yes 2885 C -> T No 3396 A -> G Yes 3562 C -> T Yes 3753 C -> T Yes

Variant protein HUMCEA_PEA_(—)1_P5 (SEQ ID NO: 551) according to the present invention has an amino acid sequence as given at the end of the application; it is encoded by transcript(s) HUMCEA_PEA_(—)1_T9 (SEQ ID NO: 503). An alignment is given to the known protein (Carcinoembryonic antigen-related cell adhesion molecule 5 precursor) at the end of the application. One or more alignments to one or more previously published protein sequences are given at the end of the application. A brief description of the relationship of the variant protein according to the present invention to each such aligned protein is as follows:

Comparison report between HUMCEA_PEA_(—)1_P5 (SEQ ID NO: 551) and CEA5_HUMAN:

1. An isolated chimeric polypeptide encoding for HUMCEA_PEA_(—)1_P5 (SEQ ID NO: 551), comprising a first amino acid sequence being at least 90% homologous to MESPSAPPHRWCIPWQRLLLTASLLTFWNPPTTAKLTIESTPFNVAEGKEVLLLVHNLPQ HLFGYSWYKGERVDGNRQIIGYVIGTQQATPGPAYSGREIIYPNASLLIQNIIQNDTGFYT LHVIKSDLVNEEATGQFRVYPELPKPSISSNNSKPVEDKDAVAFTCEPETQDATYLWWV NNQSLPVSPRLQLSNGNRTLTLFNVTRNDTASYKCETQNPVSARRSDSVILNVLYGPDA PTISPLNTSYRSGENLNLSCHAASNPPAQYSWFVNGTFQQSTQELFIPNITVNNSGSYTC QAHNSDTGLNRTTVTTITVYAEPPKPFITSNNSNPVEDEDAVALTCEPEIQNTTYLWWV NNQSLPVSPRLQLSNDNRTLTLLSVTRNDVGPYECGIQNELSVDHSDPVILNVLYGPDD PTISPSYTYYRPGVNLSLSCHAASNPPAQYSWLIDGNIQQHTQELFISNITEKNSGLYTCQ ANNSASGHSRTTVKTITVSAELPKPSISSNNSKPVEDKDAVAFTCEPEAQNTTYLWWVN GQSLPVSPRLQLSNGNRTLTLFNVTRNDARAYVCGIQNSVSANRSDPVTLDVLYGPDTP IISPPDSSYLSGANLNLSCHSASNPSPQYSWRINGIPQQHTQVLFIAKITPNNNGTYACFV SNLATGRNNSIVKSITVS corresponding to amino acids 1-675 of CEA5_HUMAN, which also corresponds to amino acids 1-675 of HUMCEA_PEA_(—)1_P5 (SEQ ID NO: 551), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence GKWLPGASASYSGVESIWFSPKSQEDIFFPSLCSMGTRKSQILS (SEQ ID NO: 1146) corresponding to amino acids 676-719 of HUMCEA_PEA_(—)1_P5 (SEQ ID NO: 551), wherein said first amino acid sequence and second amino acid sequence are contiguous and in a sequential order.

2. An isolated polypeptide encoding for a tail of HUMCEA_PEA_(—)1_P5 (SEQ ID NO: 551), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence GKWLPGASASYSGVESIWFSPKSQEDIFFPSLCSMGTRKSQILS (SEQ ID NO: 1146) in HUMCEA_PEA_(—)1_P5 (SEQ ID NO: 551).

The location of the variant protein was determined according to results from a number of different software programs and analyses, including analyses from SignalP and other specialized programs. The variant protein is believed to be located as follows with regard to the cell: secreted. The protein localization is believed to be secreted because both signal-peptide prediction programs predict that this protein has a signal peptide, and neither trans-membrane region prediction program predicts that this protein has a trans-membrane region.

Variant protein HUMCEA_PEA_(—)1_P5 (SEQ ID NO: 551) also has the following non-silent SNPs (Single Nucleotide Polymorphisms) as listed in Table 11, (given according to their position(s) on the amino acid sequence, with the alternative amino acid(s) listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein HUMCEA_PEA_(—)1_P5 (SEQ ID NO: 551) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 11 Amino acid mutations SNP position(s) on amino acid Alternative Previously sequence amino acid(s) known SNP? 63 F -> L No 80 I -> V Yes 83 V -> A Yes 137 Q -> P Yes 173 D -> N No 289 I -> T No 340 A -> D Yes 398 E -> K Yes 647 P -> No 664 R -> S Yes

The glycosylation sites of variant protein HUMCEA_PEA_(—)1_P5 (SEQ ID NO: 551), as compared to the known protein Carcinoembryonic antigen-related cell adhesion molecule 5 precursor, are described in Table 12 (given according to their position(s) on the amino acid sequence in the first column; the second column indicates whether the glycosylation site is present in the variant protein; and the last column indicates whether the position is different on the variant protein).

TABLE 12 Glycosylation site(s) Position(s) on known amino Present in Position in acid sequence variant protein? variant protein? 197 yes 197 466 yes 466 360 yes 360 288 yes 288 665 yes 665 560 yes 560 650 yes 650 480 yes 480 104 yes 104 580 yes 580 204 yes 204 115 yes 115 208 yes 208 152 yes 152 309 yes 309 432 yes 432 351 yes 351 246 yes 246 182 yes 182 612 yes 612 256 yes 256 508 yes 508 330 yes 330 274 yes 274 292 yes 292 553 yes 553 529 yes 529 375 yes 375

Variant protein HUMCEA_PEA_(—)1_P5 (SEQ ID NO: 551) is encoded by the following transcript(s): HUMCEA_PEA_(—)1_T9 (SEQ ID NO: 503), for which the sequence(s) is/are given at the end of the application. The coding portion of transcript HUMCEA_PEA_(—)1_T9 (SEQ ID NO: 503) is shown in bold; this coding portion starts at position 115 and ends at position 2271. The transcript also has the following SNPs as listed in Table 13 (given according to their position on the nucleotide sequence, with the alternative nucleic acid listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein HUMCEA_PEA_(—)1_P5 (SEQ ID NO: 551) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 13 Nucleic acid SNPs SNP position on nucleotide Alternative Previously sequence nucleic acid known SNP? 49 T -> No 273 A -> C Yes 303 T -> G No 324 T -> C Yes 352 A -> G Yes 362 T -> C Yes 524 A -> C Yes 631 G -> A No 915 A -> G No 980 T -> C No 1133 C -> A Yes 1306 G -> A Yes 1908 T -> C No 1962 C -> T No 2055 A -> No 2104 C -> A Yes 3259 T -> C Yes

Variant protein HUMCEA_PEA_(—)1_P14 (SEQ ID NO: 552) according to the present invention has an amino acid sequence as given at the end of the application; it is encoded by transcript(s) HUMCEA_PEA_(—)1_T20 (SEQ ID NO: 504). The location of the variant protein was determined according to results from a number of different software programs and analyses, including analyses from SignalP and other specialized programs. The variant protein is believed to be located as follows with regard to the cell: secreted. The protein localization is believed to be secreted because both signal-peptide prediction programs predict that this protein has a signal peptide, and neither trans-membrane region prediction program predicts that this protein has a trans-membrane region.

Variant protein HUMCEA_PEA_(—)1_P14 (SEQ ID NO: 552) also has the following non-silent SNPs (Single Nucleotide Polymorphisms) as listed in Table 14, (given according to their position(s) on the amino acid sequence, with the alternative amino acid(s) listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein HUMCEA_PEA_(—)1_P14 (SEQ ID NO: 552) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 14 Amino acid mutations SNP position(s) on amino acid Alternative Previously sequence amino acid(s) known SNP? 63 F -> L No 80 I -> V Yes 83 V -> A Yes 137 Q -> P Yes 173 D -> N No 289 I -> T No 340 A -> D Yes 398 E -> K Yes

Variant protein HUMCEA_PEA_(—)1_P14 (SEQ ID NO: 552) is encoded by the following transcript(s): HUMCEA_PEA_(—)1_T20 (SEQ ID NO: 504), for which the sequence(s) is/are given at the end of the application. The coding portion of transcript HUMCEA_PEA_(—)1_T20 (SEQ ID NO: 504) is shown in bold; this coding portion starts at position 115 and ends at position 1821. The transcript also has the following SNPs as listed in Table 15 (given according to their position on the nucleotide sequence, with the alternative nucleic acid listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein HUMCEA_PEA_(—)1_P14 (SEQ ID NO: 552) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 15 Nucleic acid SNPs SNP position on nucleotide sequence Alternative nucleic acid Previously known SNP? 49 T -> No 273 A -> C Yes 303 T -> G No 324 T -> C Yes 352 A -> G Yes 362 T -> C Yes 524 A -> C Yes 631 G -> A No 915 A -> G No 980 T -> C No 1133 C -> A Yes 1306 G -> A Yes

Variant protein HUMCEA_PEA_(—)1_P19 (SEQ ID NO: 553) according to the present invention has an amino acid sequence as given at the end of the application; it is encoded by transcript(s) HUMCEA_PEA_(—)1_T25 (SEQ ID NO: 505). An alignment is given to the known protein (Carcinoembryonic antigen-related cell adhesion molecule 5 precursor) at the end of the application. One or more alignments to one or more previously published protein sequences are given at the end of the application. A brief description of the relationship of the variant protein according to the present invention to each such aligned protein is as follows:

Comparison report between HUMCEA_PEA_(—)1_P19 (SEQ ID NO: 553) and CEA5_HUMAN:

1. An isolated chimeric polypeptide encoding for HUMCEA_PEA_(—)1_P19 (SEQ ID NO: 553), comprising a first amino acid sequence being at least 90% homologous to MESPSAPPHRWCIPWQRLLLTASLLTFWNPPTTAKLTIESTPFNVAEGKEVLLLVHNLPQ HLFGYSWYKGERVDGNRQIIGYVIGTQQATPGPAYSGREIIYPNASLLIQNIIQNDTGFYT LHVIKSDLVNEEATGQFRVYPELPKPSISSNNSKPVEDKDAVAFTCEPETQDATYLWWV NNQSLPVSPRLQLSNGNRTLTLFNVTRNDTASYKCETQNPVSARRSDSVILN corresponding to amino acids 1-232 of CEA5_HUMAN, which also corresponds to amino acids 1-232 of HUMCEA_PEA_(—)1_P19 (SEQ ID NO: 553), and a second amino acid sequence being at least 90% homologous to VLYGPDTPIISPPDSSYLSGANLNLSCHSASNPSPQYSWRINGIPQQHTQVLFIAKITPNNN GTYACFVSNLATGRNNSIVKSITVSASGTSPGLSAGATVGIMIGVLVGVALI corresponding to amino acids 589-702 of CEA5_HUMAN, which also corresponds to amino acids 233-346 of HUMCEA_PEA_(—)1_P19 (SEQ ID NO: 553), wherein said first amino acid sequence and second amino acid sequence are contiguous and in a sequential order.

2. An isolated chimeric polypeptide encoding for an edge portion of HUMCEA_PEA_(—)1_P19 (SEQ ID NO: 553), comprising a polypeptide having a length “n”, wherein n is at least about 10 amino acids in length, optionally at least about 20 amino acids in length, preferably at least about 30 amino acids in length, more preferably at least about 40 amino acids in length and most preferably at least about 50 amino acids in length, wherein at least two amino acids comprise NV, having a structure as follows: a sequence starting from any of amino acid numbers 232-x to 232; and ending at any of amino acid numbers 233+((n−2)−x), in which x varies from 0 to n−2.

The location of the variant protein was determined according to results from a number of different software programs and analyses, including analyses from SignalP and other specialized programs. The variant protein is believed to be located as follows with regard to the cell: membrane. The protein localization is believed to be membrane because of manual inspection of known protein localization and/or gene structure.

Variant protein HUMCEA_PEA_(—)1_P19 (SEQ ID NO: 553) also has the following non-silent SNPs (Single Nucleotide Polymorphisms) as listed in Table 16, (given according to their position(s) on the amino acid sequence, with the alternative amino acid(s) listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein HUMCEA_PEA_(—)1_P19 (SEQ ID NO: 553) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 16 Amino acid mutations SNP position(s) on amino acid sequence Alternative amino acid(s) Previously known SNP? 63 F -> L No 80 I -> V Yes 83 V -> A Yes 137 Q -> P Yes 173 D -> N No 291 P -> No 308 R -> S Yes 326 G -> No

The glycosylation sites of variant protein HUMCEA_PEA_(—)1_P19 (SEQ ID NO: 553), as compared to the known protein Carcinoembryonic antigen-related cell adhesion molecule 5 precursor, are described in Table 17 (given according to their position(s) on the amino acid sequence in the first column; the second column indicates whether the glycosylation site is present in the variant protein; and the last column indicates whether the position is different on the variant protein).

TABLE 17 Glycosylation site(s) Position(s) on known Present in Position in amino acid sequence variant protein? variant protein? 197 yes 197 466 no 360 no 288 no 665 yes 309 560 no 650 yes 294 480 no 104 yes 104 580 no 204 yes 204 115 yes 115 208 yes 208 152 yes 152 309 no 432 no 351 no 246 no 182 yes 182 612 yes 256 256 no 508 no 330 no 274 no 292 no 553 no 529 no 375 no

Variant protein HUMCEA_PEA_(—)1_P19 (SEQ ID NO: 553) is encoded by the following transcript(s): HUMCEA_PEA_(—)1_T25 (SEQ ID NO: 505), for which the sequence(s) is/are given at the end of the application. The coding portion of transcript HUMCEA_PEA_(—)1_T25 (SEQ ID NO: 505) is shown in bold; this coding portion starts at position 115 and ends at position 1152. The transcript also has the following SNPs as listed in Table 18 (given according to their position on the nucleotide sequence, with the alternative nucleic acid listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein HUMCEA_PEA_(—)1_P19 (SEQ ID NO: 553) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 18 Nucleic acid SNPs SNP position on nucleotide sequence Alternative nucleic acid Previously known SNP? 49 T -> No 273 A -> C Yes 303 T -> G No 324 T -> C Yes 352 A -> G Yes 362 T -> C Yes 524 A -> C Yes 631 G -> A No 840 T -> C No 894 C -> T No 987 A -> No 1036 C -> A Yes 1090 G -> No 1155 G -> No 1171 T -> A No 1172 T -> A No 1364 G -> A Yes 1417 C -> T No 1928 A -> G Yes 2094 C -> T Yes 2285 C -> T Yes

Variant protein HUMCEA_PEA_(—)1_P20 (SEQ ID NO: 554) according to the present invention has an amino acid sequence as given at the end of the application; it is encoded by transcript(s) HUMCEA_PEA_(—)1_T26 (SEQ ID NO: 506). An alignment is given to the known protein (Carcinoembryonic antigen-related cell adhesion molecule 5 precursor) at the end of the application. One or more alignments to one or more previously published protein sequences are given at the end of the application. A brief description of the relationship of the variant protein according to the present invention to each such aligned protein is as follows:

Comparison report between HUMCEA_PEA_(—)1_P20 (SEQ ID NO: 554) and CEA5_HUMAN:

1. An isolated chimeric polypeptide encoding for HUMCEA_PEA_(—)1_P20 (SEQ ID NO: 554), comprising a first amino acid sequence being at least 90% homologous to MESPSAPPHRWCIPWQRLLLTASLLTFWNPPTTAKLTIESTPFNVAEGKEVLLLVHNLPQ HLFGYSWYKGERVDGNRQIIGYVIGTQQATPGPAYSGREIIYPNASLLIQNIIQNDTGFYT LHVIKSDLVNEEATGQFRVYP corresponding to amino acids 1-142 of CEA5_HUMAN, which also corresponds to amino acids 1-142 of HUMCEA_PEA_(—)1_P20 (SEQ ID NO: 554), and a second amino acid sequence being at least 90% homologous to ELPKPSISSNNSKPVEDKDAVAFTCEPEAQNTTYLWWVNGQSLPVSPRLQLSNGNRTLT LFNVTRNDARAYVCGIQNSVSANRSDPVTLDVLYGPDTPIISPPDSSYLSGANLNLSCHS ASNPSPQYSWRINGIPQQHTQVLFIAKITPNNNGTYACFVSNLATGRNNSIVKSITVSASG TSPGLSAGATVGIMIGVLVGVALI corresponding to amino acids 499-702 of CEA5_HUMAN, which also corresponds to amino acids 143-346 of HUMCEA_PEA_(—)1_P20 (SEQ ID NO: 554), wherein said first amino acid sequence and second amino acid sequence are contiguous and in a sequential order.

2. An isolated chimeric polypeptide encoding for an edge portion of HUMCEA_PEA_(—)1_P20 (SEQ ID NO: 554), comprising a polypeptide having a length “n”, wherein n is at least about 10 amino acids in length, optionally at least about 20 amino acids in length, preferably at least about 30 amino acids in length, more preferably at least about 40 amino acids in length and most preferably at least about 50 amino acids in length, wherein at least two amino acids comprise PE, having a structure as follows: a sequence starting from any of amino acid numbers 142-x to 142; and ending at any of amino acid numbers 143+((n−2)−x), in which x varies from 0 to n−2.

The location of the variant protein was determined according to results from a number of different software programs and analyses, including analyses from SignalP and other specialized programs. The variant protein is believed to be located as follows with regard to the cell: membrane. The protein localization is believed to be membrane because of manual inspection of known protein localization and/or gene structure.

Variant protein HUMCEA_PEA_(—)1_P20 (SEQ ID NO: 554) also has the following non-silent SNPs (Single Nucleotide Polymorphisms) as listed in Table 19, (given according to their position(s) on the amino acid sequence, with the alternative amino acid(s) listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein HUMCEA_PEA_(—)1_P20 (SEQ ID NO: 554) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 19 Amino acid mutations SNP position(s) on amino acid sequence Alternative amino acid(s) Previously known SNP? 63 F -> L No 80 I -> V Yes 83 V -> A Yes 137 Q -> P Yes 291 P -> No 308 R -> S Yes 326 G -> No

The glycosylation sites of variant protein HUMCEA_PEA_(—)1_P20 (SEQ ID NO: 554), as compared to the known protein Carcinoembryonic antigen-related cell adhesion molecule 5 precursor, are described in Table 20 (given according to their position(s) on the amino acid sequence in the first column; the second column indicates whether the glycosylation site is present in the variant protein; and the last column indicates whether the position is different on the variant protein).

TABLE 20 Glycosylation site(s) Position(s) on known Present in Position in amino acid sequence variant protein? variant protein? 197 no 466 no 360 no 288 no 665 yes 309 560 yes 204 650 yes 294 480 no 104 yes 104 580 yes 224 204 no 115 yes 115 208 no 152 no 309 no 432 no 351 no 246 no 182 no 612 yes 256 256 no 508 yes 152 330 no 274 no 292 no 553 yes 197 529 yes 173 375 no

Variant protein HUMCEA_PEA_(—)1_P20 (SEQ ID NO: 554) is encoded by the following transcript(s): HUMCEA_PEA_(—)1_T26 (SEQ ID NO: 506), for which the sequence(s) is/are given at the end of the application. The coding portion of transcript HUMCEA_PEA_(—)1_T26 (SEQ ID NO: 506) is shown in bold; this coding portion starts at position 115 and ends at position 1152. The transcript also has the following SNPs as listed in Table 21 (given according to their position on the nucleotide sequence, with the alternative nucleic acid listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein HUMCEA_PEA_(—)1_P20 (SEQ ID NO: 554) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 21 Nucleic acid SNPs SNP position on nucleotide sequence Alternative nucleic acid Previously known SNP? 49 T -> No 273 A -> C Yes 303 T -> G No 324 T -> C Yes 352 A -> G Yes 362 T -> C Yes 524 A -> C Yes 840 T -> C No 894 C -> T No 987 A -> No 1036 C -> A Yes 1090 G -> No 1155 G -> No 1171 T -> A No 1172 T -> A No 1364 G -> A Yes 1417 C -> T No 1928 A -> G Yes 2094 C -> T Yes 2285 C -> T Yes

As noted above, cluster HUMCEA features 42 segment(s), which were listed in Table 2 above and for which the sequence(s) are given at the end of the application. These segment(s) are portions of nucleic acid sequence(s) which are described herein separately because they are of particular interest. A description of each segment according to the present invention is now provided.

Segment cluster HUMCEA_PEA_(—)1_node_(—)0 (SEQ ID NO: 507) according to the present invention is supported by 56 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HUMCEA_PEA_(—)1_T8 (SEQ ID NO: 502), HUMCEA_PEA_(—)1_T9 (SEQ ID NO: 503), HUMCEA_PEA_(—)1_T20 (SEQ ID NO: 504), HUMCEA_PEA_(—)1_T25 (SEQ ID NO: 505) and HUMCEA_PEA_(—)1_T26 (SEQ ID NO: 506). Table 22 below describes the starting and ending position of this segment on each transcript.

TABLE 22 Segment location on transcripts Segment Segment starting ending Transcript name position position HUMCEA_PEA_1_T8 1 178 (SEQ ID NO:502) HUMCEA_PEA_1_T9 1 178 (SEQ ID NO:503) HUMCEA_PEA_1_T20 1 178 (SEQ ID NO:504) HUMCEA_PEA_1_T25 1 178 (SEQ ID NO:505) HUMCEA_PEA_1_T26 1 178 (SEQ ID NO:506)

Segment cluster HUMCEA_PEA_(—)1_node_(—)2 (SEQ ID NO: 508) according to the present invention is supported by 83 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HUMCEA_PEA_(—)1_T8 (SEQ ID NO: 502), HUMCEA_PEA_(—)1_T9 (SEQ ID NO: 503), HUMCEA_PEA_(—)1_T20 (SEQ ID NO: 504), HUMCEA_PEA_(—)1_T25 (SEQ ID NO: 505) and HUMCEA_PEA_(—)1_T26 (SEQ ID NO: 506). Table 23 below describes the starting and ending position of this segment on each transcript.

TABLE 23 Segment location on transcripts Segment Segment starting ending Transcript name position position HUMCEA_PEA_1_T8 179 456 (SEQ ID NO:502) HUMCEA_PEA_1_T9 179 456 (SEQ ID NO:503) HUMCEA_PEA_1_T20 179 456 (SEQ ID NO:504) HUMCEA_PEA_1_T25 179 456 (SEQ ID NO:505) HUMCEA_PEA_1_T26 179 456 (SEQ ID NO:506)

Segment cluster HUMCEA_PEA_(—)1_node_(—)11 (SEQ ID NO: 509) according to the present invention is supported by 6 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HUMCEA_PEA_(—)1_T8 (SEQ ID NO: 502). Table 24 below describes the starting and ending position of this segment on each transcript.

TABLE 24 Segment location on transcripts Segment Segment Transcript name starting position ending position HUMCEA_PEA_1_T8 818 1217 (SEQ ID NO: 502)

Segment cluster HUMCEA_PEA_(—)1_node_(—)12 (SEQ ID NO: 510) according to the present invention is supported by 83 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HUMCEA_PEA_(—)1_T8 (SEQ ID NO: 502), HUMCEA_PEA_(—)1_T9 (SEQ ID NO: 503) and HUMCEA_PEA_(—)1_T20 (SEQ ID NO: 504). Table 26 below describes the starting and ending position of this segment on each transcript.

TABLE 26 Segment location on transcripts Segment Segment Transcript name starting position ending position HUMCEA_PEA_1_T8 1218 1472 (SEQ ID NO: 502) HUMCEA_PEA_1_T9 818 1072 (SEQ ID NO: 503) HUMCEA_PEA_1_T20 818 1072 (SEQ ID NO: 504)

Segment cluster HUMCEA_PEA_(—)1_node_(—)31 (SEQ ID NO: 511) according to the present invention is supported by 87 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HUMCEA_PEA_(—)1_T8 (SEQ ID NO: 502), HUMCEA_PEA_(—)1_T9 (SEQ ID NO: 503) and HUMCEA_PEA_(—)1_T20 (SEQ ID NO: 504). Table 27 below describes the starting and ending position of this segment on each transcript.

TABLE 27 Segment location on transcripts Segment Segment Transcript name starting position ending position HUMCEA_PEA_1_T8 1817 2006 (SEQ ID NO: 502) HUMCEA_PEA_1_T9 1417 1606 (SEQ ID NO: 503) HUMCEA_PEA_1_T20 1417 1606 (SEQ ID NO: 504)

Segment cluster HUMCEA_PEA_(—)1_node_(—)36 (SEQ ID NO: 512) according to the present invention is supported by 94 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HUMCEA_PEA_(—)1_T8 (SEQ ID NO: 502), HUMCEA_PEA_(—)1_T9 (SEQ ID NO: 503) and HUMCEA_PEA_(—)1_T26 (SEQ ID NO: 506). Table 28 below describes the starting and ending position of this segment on each transcript.

TABLE 28 Segment location on transcripts Segment Segment starting ending Transcript name position position HUMCEA_PEA_1_T8 2159 2285 (SEQ ID NO:502) HUMCEA_PEA_1_T9 1759 1885 (SEQ ID NO:503) HUMCEA_PEA_1_T26 691 817 (SEQ ID NO:506)

Segment cluster HUMCEA_PEA_(—)1_node_(—)44 (SEQ ID NO: 513) according to the present invention is supported by 112 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HUMCEA_PEA_(—)1_T8 (SEQ ID NO: 502), HUMCEA_PEA_(—)1_T9 (SEQ ID NO: 503), HUMCEA_PEA_(—)1_T25 (SEQ ID NO: 505) and HUMCEA_PEA_(—)1_T26 (SEQ ID NO: 506). Table 29 below describes the starting and ending position of this segment on each transcript.

TABLE 29 Segment location on transcripts Segment Segment starting ending Transcript name position position HUMCEA_PEA_1_T8 2286 2540 (SEQ ID NO:502) HUMCEA_PEA_1_T9 1886 2140 (SEQ ID NO:503) HUMCEA_PEA_1_T25 818 1072 (SEQ ID NO:505) HUMCEA_PEA_1_T26 818 1072 (SEQ ID NO:506)

Segment cluster HUMCEA_PEA_(—)1_node_(—)46 (SEQ ID NO: 514) according to the present invention is supported by 15 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HUMCEA_PEA_(—)1_T9 (SEQ ID NO: 503). Table 30 below describes the starting and ending position of this segment on each transcript.

TABLE 30 Segment location on transcripts Segment Segment starting ending Transcript name position position HUMCEA_PEA_1_T9 2174 3347 (SEQ ID NO:503)

Segment cluster HUMCEA_PEA_(—)1_node_(—)63 (SEQ ID NO: 515) according to the present invention is supported by 68 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HUMCEA_PEA_(—)1_T8 (SEQ ID NO: 502), HUMCEA_PEA_(—)1_T25 (SEQ ID NO: 505) and HUMCEA_PEA_(—)1_T26 (SEQ ID NO: 506). Table 31 below describes the starting and ending position of this segment on each transcript.

TABLE 31 Segment location on transcripts Segment Segment starting ending Transcript name position position HUMCEA_PEA_1_T8 2957 3135 (SEQ ID NO:502) HUMCEA_PEA_1_T25 1489 1667 (SEQ ID NO:505) HUMCEA_PEA_1_T26 1489 1667 (SEQ ID NO:506)

Segment cluster HUMCEA_PEA_(—)1_node_(—)65 (SEQ ID NO: 516) according to the present invention is supported by 54 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HUMCEA_PEA_(—)1_T8 (SEQ ID NO: 502), HUMCEA_PEA_(—)1_T25 (SEQ ID NO: 505) and HUMCEA_PEA_(—)1_T26 (SEQ ID NO: 506). Table 32 below describes the starting and ending position of this segment on each transcript.

TABLE 32 Segment location on transcripts Segment Segment starting ending Transcript name position position HUMCEA_PEA_1_T8 3166 3897 (SEQ ID NO:502) HUMCEA_PEA_1_T25 1698 2429 (SEQ ID NO:505) HUMCEA_PEA_1_T26 1698 2429 (SEQ ID NO:506)

Segment cluster HUMCEA_PEA_(—)1_node_(—)67 (SEQ ID NO: 517) according to the present invention is supported by 2 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HUMCEA_PEA_(—)1_T20 (SEQ ID NO: 504). Table 33 below describes the starting and ending position of this segment on each transcript.

TABLE 33 Segment location on transcripts Segment Segment starting ending Transcript name position position HUMCEA_PEA_1_T20 1607 1886 (SEQ ID NO:504)

According to an optional embodiment of the present invention, short segments related to the above cluster are also provided. These segments are up to about 120 bp in length, and so are included in a separate description.

Segment cluster HUMCEA_PEA_(—)1_node_(—)3 (SEQ ID NO: 518) according to the present invention is supported by 67 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HUMCEA_PEA_(—)1_T8 (SEQ ID NO: 502), HUMCEA_PEA_(—)1_T9 (SEQ ID NO: 503), HUMCEA_PEA_(—)1_T20 (SEQ ID NO: 504), HUMCEA_PEA_(—)1_T25 (SEQ ID NO: 505) and HUMCEA_PEA_(—)1_T26 (SEQ ID NO: 506). Table 34 below describes the starting and ending position of this segment on each transcript.

TABLE 34 Segment location on transcripts Segment Segment starting ending Transcript name position position HUMCEA_PEA_1_T8 457 538 (SEQ ID NO:502) HUMCEA_PEA_1_T9 457 538 (SEQ ID NO:503) HUMCEA_PEA_1_T20 457 538 (SEQ ID NO:504) HUMCEA_PEA_1_T25 457 538 (SEQ ID NO:505) HUMCEA_PEA_1_T26 457 538 (SEQ ID NO:506)

Segment cluster HUMCEA_PEA_(—)1_node_(—)7 (SEQ ID NO: 519) according to the present invention is supported by 73 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HUMCEA_PEA_(—)1_T8 (SEQ ID NO: 502), HUMCEA_PEA_(—)1_T9 (SEQ ID NO: 503), HUMCEA_PEA_(—)1_T20 (SEQ ID NO: 504) and HUMCEA_PEA_(—)1_T25 (SEQ ID NO: 505). Table 35 below describes the starting and ending position of this segment on each transcript.

TABLE 35 Segment location on transcripts Segment Segment starting ending Transcript name position position HUMCEA_PEA_1_T8 539 642 (SEQ ID NO:502) HUMCEA_PEA_1_T9 539 642 (SEQ ID NO:503) HUMCEA_PEA_1_T20 539 642 (SEQ ID NO:504) HUMCEA_PEA_1_T25 539 642 (SEQ ID NO:505)

Segment cluster HUMCEA_PEA_(—)1_node_(—)8 (SEQ ID NO: 520) according to the present invention is supported by 67 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HUMCEA_PEA_(—)1_T8 (SEQ ID NO: 502), HUMCEA_PEA_(—)1_T9 (SEQ ID NO: 503), HUMCEA_PEA_(—)1_T20 (SEQ ID NO: 504) and HUMCEA_PEA_(—)1_T25 (SEQ ID NO: 505). Table 36 below describes the starting and ending position of this segment on each transcript.

TABLE 36 Segment location on transcripts Segment Segment starting ending Transcript name position position HUMCEA_PEA_1_T8 643 690 (SEQ ID NO:502) HUMCEA_PEA_1_T9 643 690 (SEQ ID NO:503) HUMCEA_PEA_1_T20 643 690 (SEQ ID NO:504) HUMCEA_PEA_1_T25 643 690 (SEQ ID NO:505)

Segment cluster HUMCEA_PEA_(—)1_node_(—)9 (SEQ ID NO: 521) according to the present invention is supported by 71 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HUMCEA_PEA_(—)1_T8 (SEQ ID NO: 502), HUMCEA_PEA_(—)1_T9 (SEQ ID NO: 503), HUMCEA_PEA_(—)1_T20 (SEQ ID NO: 504) and HUMCEA_PEA_(—)1_T25 (SEQ ID NO: 505). Table 37 below describes the starting and ending position of this segment on each transcript.

TABLE 37 Segment location on transcripts Segment Segment starting ending Transcript name position position HUMCEA_PEA_1_T8 691 738 (SEQ ID NO:502) HUMCEA_PEA_1_T9 691 738 (SEQ ID NO:503) HUMCEA_PEA_1_T20 691 738 (SEQ ID NO:504) HUMCEA_PEA_1_T25 691 738 (SEQ ID NO:505)

Segment cluster HUMCEA_PEA_(—)1_node_(—)10 (SEQ ID NO: 522) according to the present invention is supported by 67 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HUMCEA_PEA_(—)1_T8 (SEQ ID NO: 502), HUMCEA_PEA_(—)1_T9 (SEQ ID NO: 503), HUMCEA_PEA_(—)1_T20 (SEQ ID NO: 504) and HUMCEA_PEA_(—)1_T25 (SEQ ID NO: 505). Table 38 below describes the starting and ending position of this segment on each transcript.

TABLE 38 Segment location on transcripts Segment Segment starting ending Transcript name position position HUMCEA_PEA_1_T8 739 817 (SEQ ID NO:502) HUMCEA_PEA_1_T9 739 817 (SEQ ID NO:503) HUMCEA_PEA_1_T20 739 817 (SEQ ID NO:504) HUMCEA_PEA_1_T25 739 817 (SEQ ID NO:505)

Segment cluster HUMCEA_PEA_(—)1_node_(—)15 (SEQ ID NO: 523) according to the present invention can be found in the following transcript(s): HUMCEA_PEA_(—)1_T8 (SEQ ID NO: 502), HUMCEA_PEA_(—)1_T9 (SEQ ID NO: 503) and HUMCEA_PEA_(—)1_T20 (SEQ ID NO: 504). Table 39 below describes the starting and ending position of this segment on each transcript.

TABLE 39 Segment location on transcripts Segment Segment starting ending Transcript name position position HUMCEA_PEA_1_T8 1473 1475 (SEQ ID NO:502) HUMCEA_PEA_1_T9 1073 1075 (SEQ ID NO:503) HUMCEA_PEA_1_T20 1073 1075 (SEQ ID NO:504)

Segment cluster HUMCEA_PEA_(—)1_node_(—)16 (SEQ ID NO: 524) according to the present invention can be found in the following transcript(s): HUMCEA_PEA_(—)1_T8 (SEQ ID NO: 502), HUMCEA_PEA_(—)1_T9 (SEQ ID NO: 503) and HUMCEA_PEA_(—)1_T20 (SEQ ID NO: 504). Table 40 below describes the starting and ending position of this segment on each transcript.

TABLE 40 Segment location on transcripts Segment Segment starting ending Transcript name position position HUMCEA_PEA_1_T8 1476 1481 (SEQ ID NO:502) HUMCEA_PEA_1_T9 1076 1081 (SEQ ID NO:503) HUMCEA_PEA_1_T20 1076 1081 (SEQ ID NO:504)

Segment cluster HUMCEA_PEA_(—)1_node_(—)17 (SEQ ID NO: 525) according to the present invention can be found in the following transcript(s): HUMCEA_PEA_(—)1_T8 (SEQ ID NO: 502), HUMCEA_PEA_(—)1_T9 (SEQ ID NO: 503) and HUMCEA_PEA_(—)1_T20 (SEQ ID NO: 504). Table 41 below describes the starting and ending position of this segment on each transcript.

TABLE 41 Segment location on transcripts Segment Segment starting ending Transcript name position position HUMCEA_PEA_1_T8 1482 1488 (SEQ ID NO:502) HUMCEA_PEA_1_T9 1082 1088 (SEQ ID NO:503) HUMCEA_PEA_1_T20 1082 1088 (SEQ ID NO:504)

Segment cluster HUMCEA_PEA_(—)1_node_(—)18 (SEQ ID NO: 526) according to the present invention can be found in the following transcript(s): HUMCEA_PEA_(—)1_T8 (SEQ ID NO: 502), HUMCEA_PEA_(—)1_T9 (SEQ ID NO: 503) and HUMCEA_PEA_(—)1_T20 (SEQ ID NO: 504). Table 42 below describes the starting and ending position of this segment on each transcript.

TABLE 42 Segment location on transcripts Segment Segment starting ending Transcript name position position HUMCEA_PEA_1_T8 1489 1506 (SEQ ID NO:502) HUMCEA_PEA_1_T9 1089 1106 (SEQ ID NO:503) HUMCEA_PEA_1_T20 1089 1106 (SEQ ID NO:504)

Segment cluster HUMCEA_PEA_(—)1_node_(—)19 (SEQ ID NO: 527) according to the present invention is supported by 69 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HUMCEA_PEA_(—)1_T8 (SEQ ID NO: 502), HUMCEA_PEA_(—)1_T9 (SEQ ID NO: 503) and HUMCEA_PEA_(—)1_T20 (SEQ ID NO: 504). Table 43 below describes the starting and ending position of this segment on each transcript.

TABLE 43 Segment location on transcripts Segment Segment starting ending Transcript name position position HUMCEA_PEA_1_T8 1507 1576 (SEQ ID NO:502) HUMCEA_PEA_1_T9 1107 1176 (SEQ ID NO:503) HUMCEA_PEA_1_T20 1107 1176 (SEQ ID NO:504)

Segment cluster HUMCEA_PEA_(—)1_node_(—)20 (SEQ ID NO: 528) according to the present invention can be found in the following transcript(s): HUMCEA_PEA_(—)1_T8 (SEQ ID NO: 502), HUMCEA_PEA_(—)1_T9 (SEQ ID NO: 503) and HUMCEA_PEA_(—)1_T20 (SEQ ID NO: 504). Table 44 below describes the starting and ending position of this segment on each transcript.

TABLE 44 Segment location on transcripts Segment Segment starting ending Transcript name position position HUMCEA_PEA_1_T8 1577 1600 (SEQ ID NO:502) HUMCEA_PEA_1_T9 1177 1200 (SEQ ID NO:503) HUMCEA_PEA_1_T20 1177 1200 (SEQ ID NO:504)

Segment cluster HUMCEA_PEA_(—)1_node_(—)21 (SEQ ID NO: 529) according to the present invention can be found in the following transcript(s): HUMCEA_PEA_(—)1_T8 (SEQ ID NO: 502), HUMCEA_PEA_(—)1_T9 (SEQ ID NO: 503) and HUMCEA_PEA_(—)1_T20 (SEQ ID NO: 504). Table 45 below describes the starting and ending position of this segment on each transcript.

TABLE 45 Segment location on transcripts Segment Segment starting ending Transcript name position position HUMCEA_PEA_1_T8 1601 1624 (SEQ ID NO:502) HUMCEA_PEA_1_T9 1201 1224 (SEQ ID NO:503) HUMCEA_PEA_1_T20 1201 1224 (SEQ ID NO:504)

Segment cluster HUMCEA_PEA_(—)1_node_(—)22 (SEQ ID NO: 530) according to the present invention is supported by 77 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HUMCEA_PEA_(—)1_T8 (SEQ ID NO: 502), HUMCEA_PEA_(—)1_T9 (SEQ ID NO: 503) and HUMCEA_PEA_(—)1_T20 (SEQ ID NO: 504). Table 46 below describes the starting and ending position of this segment on each transcript.

TABLE 46 Segment location on transcripts Segment Segment starting ending Transcript name position position HUMCEA_PEA_1_T8 1625 1702 (SEQ ID NO:502) HUMCEA_PEA_1_T9 1225 1302 (SEQ ID NO:503) HUMCEA_PEA_1_T20 1225 1302 (SEQ ID NO:504)

Segment cluster HUMCEA_PEA_(—)1_node_(—)23 (SEQ ID NO: 531) according to the present invention is supported by 72 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HUMCEA_PEA_(—)1_T8 (SEQ ID NO: 502), HUMCEA_PEA_(—)1_T9 (SEQ ID NO: 503) and HUMCEA_PEA_(—)1_T20 (SEQ ID NO: 504). Table 47 below describes the starting and ending position of this segment on each transcript.

TABLE 47 Segment location on transcripts Segment Segment starting ending Transcript name position position HUMCEA_PEA_1_T8 1703 1732 (SEQ ID NO:502) HUMCEA_PEA_1_T9 1303 1332 (SEQ ID NO:503) HUMCEA_PEA_1_T20 1303 1332 (SEQ ID NO:504)

Segment cluster HUMCEA_PEA_(—)1_node_(—)24 (SEQ ID NO: 532) according to the present invention can be found in the following transcript(s): HUMCEA_PEA_(—)1_T8 (SEQ ID NO: 502), HUMCEA_PEA_(—)1_T9 (SEQ ID NO: 503) and HUMCEA_PEA_(—)1_T20 (SEQ ID NO: 504). Table 48 below describes the starting and ending position of this segment on each transcript.

TABLE 48 Segment location on transcripts Segment Segment Transcript starting ending name position position HUMCEA_PEA_1_T8 1733 1751 (SEQ ID NO:502) HUMCEA_PEA_1_T9 1333 1351 (SEQ ID NO:503) HUMCEA_PEA_1_T20 1333 1351 (SEQ ID NO:504)

Segment cluster HUMCEA_PEA_(—)1_node_(—)27 (SEQ ID NO: 533) according to the present invention can be found in the following transcript(s): HUMCEA_PEA_(—)1_T8 (SEQ ID NO: 502), HUMCEA_PEA_(—)1_T9 (SEQ ID NO: 503) and HUMCEA_PEA_(—)1_T20 (SEQ ID NO: 504). Table 49 below describes the starting and ending position of this segment on each transcript.

TABLE 49 Segment location on transcripts Segment Segment Transcript starting ending name position position HUMCEA_PEA_1_T8 1752 1770 (SEQ ID NO:502) HUMCEA_PEA_1_T9 1352 1370 (SEQ ID NO:503) HUMCEA_PEA_1_T20 1352 1370 (SEQ ID NO:504)

Segment cluster HUMCEA_PEA_(—)1_node_(—)29 (SEQ ID NO: 534) according to the present invention can be found in the following transcript(s): HUMCEA_PEA_(—)1_T8 (SEQ ID NO: 502), HUMCEA_PEA_(—)1_T9 (SEQ ID NO: 503) and HUMCEA_PEA_(—)1_T20 (SEQ ID NO: 504). Table 50 below describes the starting and ending position of this segment on each transcript.

TABLE 50 Segment location on transcripts Segment Segment Transcript starting ending name position position HUMCEA_PEA_1_T8 1771 1788 (SEQ ID NO:502) HUMCEA_PEA_1_T9 1371 1388 (SEQ ID NO:503) HUMCEA_PEA_1_T20 1371 1388 (SEQ ID NO:504)

Segment cluster HUMCEA_PEA_(—)1_node_(—)30 (SEQ ID NO: 535) according to the present invention is supported by 67 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HUMCEA_PEA_(—)1_T8 (SEQ ID NO: 502), HUMCEA_PEA_(—)1_T9 (SEQ ID NO: 503) and HUMCEA_PEA_(—)1_T20 (SEQ ID NO: 504). Table 51 below describes the starting and ending position of this segment on each transcript.

TABLE 51 Segment location on transcripts Segment Segment Transcript starting ending name position position HUMCEA_PEA_1_T8 1789 1816 (SEQ ID NO:502) HUMCEA_PEA_1_T9 1389 1416 (SEQ ID NO:503) HUMCEA_PEA_1_T20 1389 1416 (SEQ ID NO:504)

Segment cluster HUMCEA_PEA_(—)1_node_(—)33 (SEQ ID NO: 536) according to the present invention can be found in the following transcript(s): HUMCEA_PEA_(—)1_T8 (SEQ ID NO: 502), HUMCEA_PEA_(—)1_T9 (SEQ ID NO: 503) and HUMCEA_PEA_(—)1_T26 (SEQ ID NO: 506). Table 52 below describes the starting and ending position of this segment on each transcript.

TABLE 52 Segment location on transcripts Segment Segment Transcript starting ending name position position HUMCEA_PEA_1_T8 2007 2028 (SEQ ID NO:502) HUMCEA_PEA_1_T9 1607 1628 (SEQ ID NO:503) HUMCEA_PEA_1_T26 539 560 (SEQ ID NO:506)

Segment cluster HUMCEA_PEA_(—)1_node_(—)34 (SEQ ID NO: 537) according to the present invention is supported by 80 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HUMCEA_PEA_(—)1_T8 (SEQ ID NO: 502), HUMCEA_PEA_(—)1_T9 (SEQ ID NO: 503) and HUMCEA_PEA_(—)1_T26 (SEQ ID NO: 506). Table 53 below describes the starting and ending position of this segment on each transcript.

TABLE 53 Segment location on transcripts Segment Segment Transcript starting ending name position position HUMCEA_PEA_1_T8 2029 2110 (SEQ ID NO:502) HUMCEA_PEA_1_T9 1629 1710 (SEQ ID NO:503) HUMCEA_PEA_1_T26 561 642 (SEQ ID NO:506)

Segment cluster HUMCEA_PEA_(—)1_node_(—)35 (SEQ ID NO: 538) according to the present invention is supported by 75 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HUMCEA_PEA_(—)1_T8 (SEQ ID NO: 502), HUMCEA_PEA_(—)1_T9 (SEQ ID NO: 503) and HUMCEA_PEA_(—)1_T26 (SEQ ID NO: 506). Table 54 below describes the starting and ending position of this segment on each transcript.

TABLE 54 Segment location on transcripts Segment Segment Transcript starting ending name position position HUMCEA_PEA_1_T8 2111 2158 (SEQ ID NO:502) HUMCEA_PEA_1_T9 1711 1758 (SEQ ID NO:503) HUMCEA_PEA_1_T26 643 690 (SEQ ID NO:506)

Segment cluster HUMCEA_PEA_(—)1_node_(—)45 (SEQ ID NO: 539) according to the present invention is supported by 9 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HUMCEA_PEA_(—)1_T9 (SEQ ID NO: 503). Table 55 below describes the starting and ending position of this segment on each transcript.

TABLE 55 Segment location on transcripts Segment Segment Transcript starting ending name position position HUMCEA_PEA_1_T9 2141 2173 (SEQ ID NO:503)

Segment cluster HUMCEA_PEA_(—)1_node_(—)50 (SEQ ID NO: 540) according to the present invention is supported by 64 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HUMCEA_PEA_(—)1_T8 (SEQ ID NO: 502), HUMCEA_PEA_(—)1_T25 (SEQ ID NO: 505) and HUMCEA_PEA_(—)1_T26 (SEQ ID NO: 506). Table 56 below describes the starting and ending position of this segment on each transcript.

TABLE 56 Segment location on transcripts Segment Segment Transcript starting ending name position position HUMCEA_PEA_1_T8 2541 2567 (SEQ ID NO:502) HUMCEA_PEA_1_T25 1073 1099 (SEQ ID NO:505) HUMCEA_PEA_1_T26 1073 1099 (SEQ ID NO:506)

Segment cluster HUMCEA_PEA_(—)1_node_(—)51 (SEQ ID NO: 541) according to the present invention is supported by 88 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HUMCEA_PEA_(—)1_T8 (SEQ ID NO: 502), HUMCEA_PEA_(—)1_T25 (SEQ ID NO: 505) and HUMCEA_PEA_(—)1_T26 (SEQ ID NO: 506). Table 57 below describes the starting and ending position of this segment on each transcript.

TABLE 57 Segment location on transcripts Segment Segment Transcript starting ending name position position HUMCEA_PEA_1_T8 2568 2659 (SEQ ID NO:502) HUMCEA_PEA_1_T25 1100 1191 (SEQ ID NO:505) HUMCEA_PEA_1_T26 1100 1191 (SEQ ID NO:506)

Segment cluster HUMCEA_PEA_(—)1_node_(—)56 (SEQ ID NO: 542) according to the present invention is supported by 75 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HUMCEA_PEA_(—)1_T8 (SEQ ID NO: 502), HUMCEA_PEA_(—)1_T25 (SEQ ID NO: 505) and HUMCEA_PEA_(—)1_T26 (SEQ ID NO: 506). Table 58 below describes the starting and ending position of this segment on each transcript.

TABLE 58 Segment location on transcripts Segment Segment Transcript starting ending name position position HUMCEA_PEA_1_T8 2660 2685 (SEQ ID NO:502) HUMCEA_PEA_1_T25 1192 1217 (SEQ ID NO:505) HUMCEA_PEA_1_T26 1192 1217 (SEQ ID NO:506)

Segment cluster HUMCEA_PEA_(—)1_node_(—)57 (SEQ ID NO: 543) according to the present invention is supported by 82 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HUMCEA_PEA_(—)1_T8 (SEQ ID NO: 502), HUMCEA_PEA_(—)1_T25 (SEQ ID NO: 505) and HUMCEA_PEA_(—)1_T26 (SEQ ID NO: 506). Table 59 below describes the starting and ending position of this segment on each transcript.

TABLE 59 Segment location on transcripts Segment Segment Transcript starting ending name position position HUMCEA_PEA_1_T8 2686 2786 (SEQ ID NO:502) HUMCEA_PEA_1_T25 1218 1318 (SEQ ID NO:505) HUMCEA_PEA_1_T26 1218 1318 (SEQ ID NO:506)

Segment cluster HUMCEA_PEA_(—)1_node_(—)58 (SEQ ID NO: 544) according to the present invention is supported by 63 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HUMCEA_PEA_(—)1_T8 (SEQ ID NO: 502), HUMCEA_PEA_(—)1_T25 (SEQ ID NO: 505) and HUMCEA_PEA_(—)1_T26 (SEQ ID NO: 506). Table 60 below describes the starting and ending position of this segment on each transcript.

TABLE 60 Segment location on transcripts Segment Segment Transcript starting ending name position position HUMCEA_PEA_1_T8 2787 2820 (SEQ ID NO:502) HUMCEA_PEA_1_T25 1319 1352 (SEQ ID NO:505) HUMCEA_PEA_1_T26 1319 1352 (SEQ ID NO:506)

Segment cluster HUMCEA_PEA_(—)1_node_(—)60 (SEQ ID NO: 545) according to the present invention is supported by 55 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HUMCEA_PEA_(—)1_T8 (SEQ ID NO: 502), HUMCEA_PEA_(—)1_T25 (SEQ ID NO: 505) and HUMCEA_PEA_(—)1_T26 (SEQ ID NO: 506). Table 61 below describes the starting and ending position of this segment on each transcript.

TABLE 61 Segment location on transcripts Segment Segment Transcript starting ending name position position HUMCEA_PEA_1_T8 2821 2864 (SEQ ID NO:502) HUMCEA_PEA_1_T25 1353 1396 (SEQ ID NO:505) HUMCEA_PEA_1_T26 1353 1396 (SEQ ID NO:506)

Segment cluster HUMCEA_PEA_(—)1_node_(—)61 (SEQ ID NO: 546) according to the present invention can be found in the following transcript(s): HUMCEA_PEA_(—)1_T8 (SEQ ID NO: 502), HUMCEA_PEA_(—)1_T25 (SEQ ID NO: 505) and HUMCEA_PEA_(—)1_T26 (SEQ ID NO: 506). Table 62 below describes the starting and ending position of this segment on each transcript.

TABLE 62 Segment location on transcripts Segment Segment Transcript starting ending name position position HUMCEA_PEA_1_T8 2865 2868 (SEQ ID NO:502) HUMCEA_PEA_1_T25 1397 1400 (SEQ ID NO:505) HUMCEA_PEA_1_T26 1397 1400 (SEQ ID NO:506)

Segment cluster HUMCEA_PEA_(—)1_node_(—)62 (SEQ ID NO: 547) according to the present invention is supported by 60 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HUMCEA_PEA_(—)1_T8 (SEQ ID NO: 502), HUMCEA_PEA_(—)1_T25 (SEQ ID NO: 505) and HUMCEA_PEA_(—)1_T26 (SEQ ID NO: 506). Table 63 below describes the starting and ending position of this segment on each transcript.

TABLE 63 Segment location on transcripts Segment Segment Transcript starting ending name position position HUMCEA_PEA_1_T8 2869 2956 (SEQ ID NO:502) HUMCEA_PEA_1_T25 1401 1488 (SEQ ID NO:505) HUMCEA_PEA_1_T26 1401 1488 (SEQ ID NO:506)

Segment cluster HUMCEA_PEA_(—)1_node_(—)64 (SEQ ID NO: 548) according to the present invention is supported by 45 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HUMCEA_PEA_(—)1_T8 (SEQ ID NO: 502), HUMCEA_PEA_(—)1_T25 (SEQ ID NO: 505) and HUMCEA_PEA_(—)1_T26 (SEQ ID NO: 506). Table 64 below describes the starting and ending position of this segment on each transcript.

TABLE 64 Segment location on transcripts Segment Segment Transcript starting ending name position position HUMCEA_PEA_1_T8 3136 3165 (SEQ ID NO:502) HUMCEA_PEA_1_T25 1668 1697 (SEQ ID NO:505) HUMCEA_PEA_1_T26 1668 1697 (SEQ ID NO:506) Variant protein alignment to the previously known protein: Sequence name: CEA5_HUMAN Sequence documentation:

Alignment of: HUMCEA_PEA_(—)1_P4 (SEQ ID NO: 550) x CEA5_HUMAN ..

Alignment segment 1/1:

Quality: 2320.00 Escore: 0 Matching length: 234 Total length: 234 Matching Percent Similarity: 100.00 Matching Percent Identity: 100.00 Total Percent Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0

Alignment:

Sequence name: CEA5_HUMAN Sequence documentation:

Alignment of: HUMCEA_PEA_(—)1_P5 (SEQ ID NO: 551) x CEA5_HUMAN ..

Alignment segment 1/1:

Quality: 6692.00 Escore: 0 Matching length: 675 Total length: 675 Matching Percent Similarity: 100.00 Matching Percent Identity: 100.00 Total Percent Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0

Alignment:

Sequence name: CEA5_HUMAN Sequence documentation:

Alignment of: HUMCEA_PEA_(—)1_P19 (SEQ ID NO: 553) x CEA5_HUMAN ..

Alignment segment 1/1:

Quality: 3298.00 Escore: 0 Matching length: 346 Total length: 702 Matching Percent Similarity: 100.00 Matching Percent Identity: 100.00 Total Percent Similarity: 49.29 Total Percent Identity: 49.29 Gaps: 1

Alignment:

Sequence name: CEA5_HUMAN Sequence documentation:

Alignment of: HUMCEA_PEA_(—)1_P20 (SEQ ID NO: 554) x CEA5_HUMAN ..

Alignment segment 1/1:

Quality: 3294.00 Escore: 0 Matching length: 346 Total length: 702 Matching Percent Similarity: 100.00 Matching Percent Identity: 100.00 Total Percent Similarity: 49.29 Total Percent Identity: 49.29 Gaps: 1

Alignment:

Description for Cluster Humedf

Cluster HUMEDF features 3 transcript(s) and 8 segment(s) of interest, the names for which are given in Tables 1 and 2, respectively, the sequences themselves are given at the end of the application. The selected protein variants are given in table 3.

TABLE 1 Transcripts of interest SEQ ID Transcript Name NO: HUMEDF_PEA_2_T5 555 HUMEDF_PEA_2_T10 556 HUMEDF_PEA_2_T11 557

TABLE 2 Segments of interest SEQ ID Segment Name NO: HUMEDF_PEA_2_node_6 558 HUMEDF_PEA_2_node_11 559 HUMEDF_PEA_2_node_18 560 HUMEDF_PEA_2_node_19 561 HUMEDF_PEA_2_node_22 562 HUMEDF_PEA_2_node_2 563 HUMEDF_PEA_2_node_8 564 HUMEDF_PEA_2_node_20 565

TABLE 3 Proteins of interest SEQ ID Corresponding Protein Name NO: Transcript(s) HUMEDF_PEA_2_P5 567 HUMEDF_PEA_2_T10 (SEQ ID NO:556) HUMEDF_PEA_2_P6 568 HUMEDF_PEA_2_T11 (SEQ ID NO:557) HUMEDF_PEA_2_P8 569 HUMEDE_PEA_2_T5 (SEQ ID NO:555)

These sequences are variants of the known protein Inhibin beta A chain precursor (SwissProt accession identifier IHBA_HUMAN; known also according to the synonyms Activin beta-A chain; Erythroid differentiation protein; EDF), SEQ ID NO: 566, referred to herein as the previously known protein.

Protein Inhibin beta A chain precursor is known or believed to have the following function(s): inhibins and activins inhibit and activate, respectively, the secretion of follitropin by the pituitary gland. Inhibins/activins are involved in regulating a number of diverse functions such as hypothalamic and pituitary hormone secretion, gonadal hormone secretion, germ cell development and maturation, erythroid differentiation, insulin secretion, nerve cell survival, embryonic axial development or bone growth, depending on their subunit composition. Inhibins appear to oppose the functions of activins. The sequence for protein Inhibin beta A chain precursor is given at the end of the application, as “Inhibin beta A chain precursor amino acid sequence”. Known polymorphisms for this sequence are as shown in Table 4.

TABLE 4 Amino acid mutations for Known Protein SNP position(s) on amino acid sequence Comment 377-379 RMR -> AC

The previously known protein also has the following indication(s) and/or potential therapeutic use(s): Cancer; Osteoporosis; Contraceptive, female; Contraceptive, male; Diagnosis, cancer. It has been investigated for clinical/therapeutic use in humans, for example as a target for an antibody or small molecule, and/or as a direct therapeutic; available information related to these investigations is as follows. Potential pharmaceutically related or therapeutically related activity or activities of the previously known protein are as follows: Erythroid differentiation factor agonist; Follicle-stimulating hormone agonist; Growth factor agonist; Inhibin agonist; Interleukin 6 antagonist; Osteoblast stimulant. A therapeutic role for a protein represented by the cluster has been predicted. The cluster was assigned this field because there was information in the drug database or the public databases (e.g., described herein above) that this protein, or part thereof, is used or can be used for a potential therapeutic indication: Haematological; Female contraceptive; Male contraceptive; Antianaemic; Osteoporosis treatment; Fertility enhancer; Anticancer; Diagnostic; Antisickling; Neurological; Alimentary/Metabolic.

The following GO Annotation(s) apply to the previously known protein. The following annotation(s) were found: skeletal development; ovarian follicle development; induction of apoptosis; defense response; cell cycle arrest; cell surface receptor linked signal transduction; cell-cell signaling; neurogenesis; mesoderm development; cell growth and/or maintenance; response to external stimulus; cell differentiation; erythrocyte differentiation; growth, which are annotation(s) related to Biological Process; defense/immunity protein; cytokine; transforming growth factor beta receptor ligand; hormone; protein binding; growth factor; activin inhibitor, which are annotation(s) related to Molecular Function; and extracellular, which are annotation(s) related to Cellular Component.

The GO assignment relies on information from one or more of the SwissProt/TremBl Protein knowledgebase, available from <http://www.expasy.ch/sprot/>; or Locuslink, available from <http://www.ncbi.nlm.nih.gov/projects/LocusLink/>.

As noted above, cluster HUMEDF features 3 transcript(s), which were listed in Table 1 above. These transcript(s) encode for protein(s) which are variant(s) of protein Inhibin beta A chain precursor. A description of each variant protein according to the present invention is now provided.

Variant protein HUMEDF_PEA_(—)2_P5 (SEQ ID NO: 567) according to the present invention has an amino acid sequence as given at the end of the application; it is encoded by transcript(s) HUMEDF_PEA_(—)2_T10 (SEQ ID NO: 556). An alignment is given to the known protein (Inhibin beta A chain precursor) at the end of the application. One or more alignments to one or more previously published protein sequences are given at the end of the application. A brief description of the relationship of the variant protein according to the present invention to each such aligned protein is as follows:

Comparison report between HUMEDF_PEA_(—)2_P5 (SEQ ID NO: 567) and IHBA_HUMAN:

1. An isolated chimeric polypeptide encoding for HUMEDF_PEA_(—)2_P5 (SEQ ID NO: 567), comprising a first amino acid sequence being at least 90% homologous to MPLLWLRGFLLASCWIIVRSSPTPGSEGHSAAPDCPSCALAALPKDVPNSQPEMVEAVK KHILNMLHLKKRPDVTQPVPKAALLNAIRKLHVGKVGENGYVEIEDDIGRRAEMNELM EQTSEIITFAESGT corresponding to amino acids 1-131 of IHBA_HUMAN, which also corresponds to amino acids 1-131 of HUMEDF_PEA_(—)2_P5 (SEQ ID NO: 567), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence VKS (SEQ ID NO: 1147) corresponding to amino acids 132-134 of HUMEDF_PEA_(—)2_P5 (SEQ ID NO: 567), wherein said first amino acid sequence and second amino acid sequence are contiguous and in a sequential order.

2. An isolated polypeptide encoding for a tail of HUMEDF_PEA_(—)2_P5 (SEQ ID NO: 567), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence VKS (SEQ ID NO: 1147) in HUMEDF_PEA_(—)2_P5 (SEQ ID NO: 567).

The location of the variant protein was determined according to results from a number of different software programs and analyses, including analyses from SignalP and other specialized programs. The variant protein is believed to be located as follows with regard to the cell: secreted. The protein localization is believed to be secreted because both signal-peptide prediction programs predict that this protein has a signal peptide, and neither trans-membrane region prediction program predicts that this protein has a trans-membrane region.

The glycosylation sites of variant protein HUMEDF_PEA_(—)2_P5 (SEQ ID NO: 567), as compared to the known protein Inhibin beta A chain precursor, are described in Table 5 (given according to their position(s) on the amino acid sequence in the first column; the second column indicates whether the glycosylation site is present in the variant protein; and the last column indicates whether the position is different on the variant protein).

TABLE 5 Glycosylation site(s) Position(s) on known amino Present in acid sequence variant protein? 165 no

Variant protein HUMEDF_PEA_(—)2_P5 (SEQ ID NO: 567) is encoded by the following transcript(s): HUMEDF_PEA_(—)2_T10 (SEQ ID NO: 556), for which the sequence(s) is/are given at the end of the application. The coding portion of transcript HUMEDF_PEA_(—)2_T10 (SEQ ID NO: 556) is shown in bold; this coding portion starts at position 246 and ends at position 647. The transcript also has the following SNPs as listed in Table 6 (given according to their position on the nucleotide sequence, with the alternative nucleic acid listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein HUMEDF_PEA_(—)2_P5 (SEQ ID NO: 567) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 6 Nucleic acid SNPs SNP position on nucleotide sequence Alternative nucleic acid Previously known SNP? 139 -> A No 170 A -> No 347 C -> T No 362 G -> C No

Variant protein HUMEDF_PEA_(—)2_P6 (SEQ ID NO: 568) according to the present invention has an amino acid sequence as given at the end of the application; it is encoded by transcript(s) HUMEDF_PEA_(—)2_T11 (SEQ ID NO: 557). An alignment is given to the known protein (Inhibin beta A chain precursor) at the end of the application. One or more alignments to one or more previously published protein sequences are given at the end of the application. A brief description of the relationship of the variant protein according to the present invention to each such aligned protein is as follows:

Comparison report between HUMEDF_PEA_(—)2_P6 (SEQ ID NO: 568) and IHBA_HUMAN:

1. An isolated chimeric polypeptide encoding for HUMEDF_PEA_(—)2_P6 (SEQ ID NO: 568), comprising a first amino acid sequence being at least 90% homologous to MPLLWLRGFLLASCWIIVRSSPTPGSEGHSAAPDCPSCALAALPKDVPNSQPEMVEAVK KHILNMLHLKKRPDVTQPVPKAALLNAIRKLHVGKVGENGYVEIEDDIGRRAEMNELM EQTSEIITFAESG corresponding to amino acids 1-130 of IHBA_HUMAN, which also corresponds to amino acids 1-130 of HUMEDF_PEA_(—)2_P6 (SEQ ID NO: 568), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence HSEA (SEQ ID NO: 1148) corresponding to amino acids 131-134 of HUMEDF_PEA_(—)2_P6 (SEQ ID NO: 568), wherein said first amino acid sequence and second amino acid sequence are contiguous and in a sequential order.

2. An isolated polypeptide encoding for a tail of HUMEDF_PEA_(—)2_P6 (SEQ ID NO: 568), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence HSEA (SEQ ID NO: 1148) in HUMEDF_PEA_(—)2_P6 (SEQ ID NO: 568).

The location of the variant protein was determined according to results from a number of different software programs and analyses, including analyses from SignalP and other specialized programs. The variant protein is believed to be located as follows with regard to the cell: secreted. The protein localization is believed to be secreted because both signal-peptide prediction programs predict that this protein has a signal peptide, and neither trans-membrane region prediction program predicts that this protein has a trans-membrane region.

The glycosylation sites of variant protein HUMEDF_PEA_(—)2_P6 (SEQ ID NO: 568), as compared to the known protein Inhibin beta A chain precursor, are described in Table 7 (given according to their position(s) on the amino acid sequence in the first column; the second column indicates whether the glycosylation site is present in the variant protein; and the last column indicates whether the position is different on the variant protein).

TABLE 7 Glycosylation site(s) Position(s) on known Present in amino acid sequence variant protein? 165 no

Variant protein HUMEDF_PEA_(—)2_P6 (SEQ ID NO: 568) is encoded by the following transcript(s): HUMEDF_PEA_(—)2_T11 (SEQ ID NO: 557), for which the sequence(s) is/are given at the end of the application. The coding portion of transcript HUMEDF_PEA_(—)2_T11 (SEQ ID NO: 557) is shown in bold; this coding portion starts at position 246 and ends at position 647. The transcript also has the following SNPs as listed in Table 8 (given according to their position on the nucleotide sequence, with the alternative nucleic acid listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein HUMEDF_PEA_(—)2_P6 (SEQ ID NO: 568) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 8 Nucleic acid SNPs SNP position on nucleotide sequence Alternative nucleic acid Previously known SNP? 139 -> A No 170 A -> No 347 C -> T No 362 G -> C No

Variant protein HUMEDF_PEA_(—)2_P8 (SEQ ID NO: 569) according to the present invention has an amino acid sequence as given at the end of the application; it is encoded by transcript(s) HUMEDF_PEA_(—)2_T5 (SEQ ID NO: 555). An alignment is given to the known protein (Inhibin beta A chain precursor) at the end of the application. One or more alignments to one or more previously published protein sequences are given at the end of the application. A brief description of the relationship of the variant protein according to the present invention to each such aligned protein is as follows:

Comparison report between HUMEDF_PEA_(—)2_P8 (SEQ ID NO: 569) and IHBA_HUMAN:

1. An isolated chimeric polypeptide encoding for HUMEDF_PEA_(—)2_P8 (SEQ ID NO: 569), comprising a first amino acid sequence being at least 90% homologous to MPLLWLRGFLLASCWIIVRSSPTPGSEGHSAAPDCPSCALAALPKDVPNSQPEMVEAVK KHILNMLHLKKRPDVTQPVPKAALLNAIRKLHVGKVGENGYVEIEDDIGRRAEMNELM EQTSEIITFAESGT corresponding to amino acids 1-131 of IHBA_HUMAN, which also corresponds to amino acids 1-131 of HUMEDF_PEA_(—)2_P8 (SEQ ID NO: 569), and a second amino acid sequence being at least 70%, optionally at least 80%, preferably at least 85%, more preferably at least 90% and most preferably at least 95% homologous to a polypeptide having the sequence VKS (SEQ ID NO: 1147) corresponding to amino acids 132-134 of HUMEDF_PEA_(—)2_P8 (SEQ ID NO: 569), wherein said first amino acid sequence and second amino acid sequence are contiguous and in a sequential order.

2. An isolated polypeptide encoding for a tail of HUMEDF_PEA_(—)2_P8 (SEQ ID NO: 569), comprising a polypeptide being at least 70%, optionally at least about 80%, preferably at least about 85%, more preferably at least about 90% and most preferably at least about 95% homologous to the sequence VKS (SEQ ID NO: 1147) in HUMEDF_PEA_(—)2_P8 (SEQ ID NO: 569).

The location of the variant protein was determined according to results from a number of different software programs and analyses, including analyses from SignalP and other specialized programs. The variant protein is believed to be located as follows with regard to the cell: secreted. The protein localization is believed to be secreted because both signal-peptide prediction programs predict that this protein has a signal peptide, and neither trans-membrane region prediction program predicts that this protein has a trans-membrane region.

The glycosylation sites of variant protein HUMEDF_PEA_(—)2_P8 (SEQ ID NO: 569), as compared to the known protein Inhibin beta A chain precursor, are described in Table 9 (given according to their position(s) on the amino acid sequence in the first column; the second column indicates whether the glycosylation site is present in the variant protein; and the last column indicates whether the position is different on the variant protein).

TABLE 9 Glycosylation site(s) Position(s) on known Present in amino acid sequence variant protein? 165 no

Variant protein HUMEDF_PEA_(—)2_P8 (SEQ ID NO: 569) is encoded by the following transcript(s): HUMEDF_PEA_(—)2_T5 (SEQ ID NO: 555), for which the sequence(s) is/are given at the end of the application. The coding portion of transcript HUMEDF_PEA_(—)2_T5 (SEQ ID NO: 555) is shown in bold; this coding portion starts at position 246 and ends at position 647. The transcript also has the following SNPs as listed in Table 10 (given according to their position on the nucleotide sequence, with the alternative nucleic acid listed; the last column indicates whether the SNP is known or not; the presence of known SNPs in variant protein HUMEDF_PEA_(—)2_P8 (SEQ ID NO: 569) sequence provides support for the deduced sequence of this variant protein according to the present invention).

TABLE 10 Nucleic acid SNPs SNP position on nucleotide sequence Alternative nucleic acid Previously known SNP? 139 -> A No 170 A -> No 347 C -> T No 362 G -> C No 878 G -> No 1028 G -> No 1216 A -> G No 1552 A -> No 1627 G -> T No 1735 A -> No

As noted above, cluster HUMEDF features 8 segment(s), which were listed in Table 2 above and for which the sequence(s) are given at the end of the application. These segment(s) are portions of nucleic acid sequence(s) which are described herein separately because they are of particular interest. A description of each segment according to the present invention is now provided.

Segment cluster HUMEDF_PEA_(—)2_node_(—)6 (SEQ ID NO: 558) according to the present invention is supported by 65 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HUMEDF_PEA_(—)2_T5 (SEQ ID NO: 555), HUMEDF_PEA_(—)2_T10 (SEQ ID NO: 556) and HUMEDF_PEA_(—)2_T11 (SEQ ID NO: 557). Table 11 below describes the starting and ending position of this segment on each transcript.

TABLE 11 Segment location on transcripts Segment Segment Transcript starting ending name position position HUMEDF_PEA_2_T5 103 633 (SEQ ID NO:555) HUMEDF_PEA_2_T10 103 633 (SEQ ID NO:556) HUMEDF_PEA_2_T11 103 633 (SEQ ID NO:557)

Segment cluster HUMEDF_PEA_(—)2_node_(—)11 (SEQ ID NO: 559) according to the present invention is supported by 5 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HUMEDF_PEA_(—)2_T10 (SEQ ID NO: 556) and HUMEDF_PEA_(—)2_T11 (SEQ ID NO: 557). Table 12 below describes the starting and ending position of this segment on each transcript.

TABLE 12 Segment location on transcripts Segment Segment Transcript starting ending name position position HUMEDF_PEA_2_T10 718 1129 (SEQ ID NO:556) HUMEDF_PEA_2_T11 634 1045 (SEQ ID NO:557)

Segment cluster HUMEDF_PEA_(—)2_node_(—)18 (SEQ ID NO: 560) according to the present invention is supported by 90 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HUMEDF_PEA_(—)2_T5 (SEQ ID NO: 555). Table 13 below describes the starting and ending position of this segment on each transcript.

TABLE 13 Segment location on transcripts Segment Segment Transcript starting ending name position position HUMEDF_PEA_2_T5 718 1660 (SEQ ID NO:555)

Segment cluster HUMEDF_PEA_(—)2_node_(—)19 (SEQ ID NO: 561) according to the present invention is supported by 86 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HUMEDF_PEA_(—)2_T5 (SEQ ID NO: 555). Table 14 below describes the starting and ending position of this segment on each transcript.

TABLE 14 Segment location on transcripts Segment Segment Transcript starting ending name position position HUMEDF_PEA_2_T5 1661 4414 (SEQ ID NO:555)

Segment cluster HUMEDF_PEA_(—)2_node_(—)22 (SEQ ID NO: 562) according to the present invention is supported by 89 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HUMEDF_PEA_(—)2_T5 (SEQ ID NO: 555). Table 15 below describes the starting and ending position of this segment on each transcript.

TABLE 15 Segment location on transcripts Segment Segment Transcript starting ending name position position HUMEDF_PEA_2_T5 4474 6164 (SEQ ID NO:555)

According to an optional embodiment of the present invention, short segments related to the above cluster are also provided. These segments are up to about 120 bp in length, and so are included in a separate description.

Segment cluster HUMEDF_PEA_(—)2_node_(—)2 (SEQ ID NO: 563) according to the present invention is supported by 31 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HUMEDF_PEA_(—)2_T5 (SEQ ID NO: 555), HUMEDF_PEA_(—)2_T10(SEQ ID NO: 556) and HUMEDF_PEA_(—)2_T11 (SEQ ID NO: 557). Table 16 below describes the starting and ending position of this segment on each transcript.

TABLE 16 Segment location on transcripts Segment Segment Transcript starting ending name position position HUMEDF_PEA_2_T5 1 102 (SEQ ID NO:555) HUMEDF_PEA_2_T10 1 102 (SEQ ID NO:556) HUMEDF_PEA_2_T11 1 102 (SEQ ID NO:557)

Segment cluster HUMEDF_PEA_(—)2_node_(—)8 (SEQ ID NO: 564) according to the present invention is supported by 5 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HUMEDF_PEA_(—)2_T5 (SEQ ID NO: 555) and HUMEDF_PEA_(—)2_T10 (SEQ ID NO: 556). Table 17 below describes the starting and ending position of this segment on each transcript.

TABLE 17 Segment location on transcripts Segment Segment Transcript starting ending name position position HUMEDF_PEA_2_T5 634 717 (SEQ ID NO:555) HUMEDF_PEA_2_T10 634 717 (SEQ ID NO:556)

Segment cluster HUMEDF_PEA_(—)2_node_(—)20 (SEQ ID NO: 565) according to the present invention is supported by 18 libraries. The number of libraries was determined as previously described. This segment can be found in the following transcript(s): HUMEDF_PEA_(—)2_T5 (SEQ ID NO: 555). Table 18 below describes the starting and ending position of this segment on each transcript.

TABLE 18 Segment location on transcripts Segment Segment Transcript starting ending name position position HUMEDF_PEA_2_T5 4415 4473 (SEQ ID NO:555) Variant protein alignment to the previously known protein: Sequence name: IHBA_HUMAN Sequence documentation:

Alignment of: HUMEDF_PEA_(—)2_P5 (SEQ ID NO: 567) x IHBA_HUMAN ..

Alignment segment 1/1:

Quality: 1285.00 Escore: 0 Matching length: 133 Total length: 133 Matching Percent Similarity: 99.25 Matching Percent Identity: 98.50 Total Percent Similarity: 99.25 Total Percent Identity: 98.50 Gaps: 0

Alignment:

Sequence name: IHBA_HUMAN Sequence documentation:

Alignment of: HUMEDF_PEA_(—)2_P6 (SEQ ID NO: 568) x IHBA_HUMAN ..

Alignment segment 1/1:

Quality: 1275.00 Escore: 0 Matching length: 130 Total length: 130 Matching Percent Similarity: 100.00 Matching Percent Identity: 100.00 Total Percent Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0

Alignment:

Sequence name: IHBA_HUMAN Sequence documentation:

Alignment of: HUMEDF_PEA_(—)2_P8 (SEQ ID NO: 569) x IHBA_HUMAN ..

Alignment segment 1/1:

Quality: 1285.00 Escore: 0 Matching length: 133 Total length: 133 Matching Percent Similarity: 99.25 Matching Percent Identity: 98.50 Total Percent Similarity: 99.25 Total Percent Identity: 98.50 Gaps: 0

Alignment:

It is appreciated that certain features of the invention, which are, for clarity, described in the context of separate embodiments, may also be provided in combination in a single embodiment. Conversely, various features of the invention, which are, for brevity, described in the context of a single embodiment, may also be provided separately or in any suitable subcombination.

Although the invention has been described in conjunction with specific embodiments thereof, it is evident that many alternatives, modifications and variations will be apparent to those skilled in the art. Accordingly, it is intended to embrace all such alternatives, modifications and variations that fall within the spirit and broad scope of the appended claims. All publications, patents and patent applications mentioned in this specification are herein incorporated in their entirety by reference into the specification, to the same extent as if each individual publication, patent or patent application was specifically and individually indicated to be incorporated herein by reference. In addition, citation or identification of any reference in this application shall not be construed as an admission that such reference is available as prior art to the present invention. 

1. An isolated polynucleotide consisting of the sequence of H61775_T21 (SEQ ID NO:1).
 2. An isolated node consisting of the sequence of: H61775_node_(—)8 (SEQ ID NO:6).
 3. An amplicon consisting of the sequence of SEQ ID NO:957.
 4. A primer pair, comprising a pair of isolated oligonucleotides to amplify said amplicon of claim 3, said pair of isolated oligonucleotides consisting of the sequences of SEQ ID NOs. 955 and
 956. 5-30. (canceled) 